CN1861592A - Soybean extract containing lecithin and isoflavone and its preparation process - Google Patents

Soybean extract containing lecithin and isoflavone and its preparation process Download PDF

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Publication number
CN1861592A
CN1861592A CNA2005100109984A CN200510010998A CN1861592A CN 1861592 A CN1861592 A CN 1861592A CN A2005100109984 A CNA2005100109984 A CN A2005100109984A CN 200510010998 A CN200510010998 A CN 200510010998A CN 1861592 A CN1861592 A CN 1861592A
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China
Prior art keywords
extraction
soybean
extract
pressure
entrainment agent
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CNA2005100109984A
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Inventor
邱明华
李忠荣
张壮鑫
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Zhongke Bencao Sci & Tech Co Ltd Yunnan
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Zhongke Bencao Sci & Tech Co Ltd Yunnan
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Priority to CNA2005100109984A priority Critical patent/CN1861592A/en
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids

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  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Beans For Foods Or Fodder (AREA)

Abstract

A soybean extract containing lecithin (70% or more) and isoflavone (8% or more) is prepared from soybean through extracting by supercritical CO2. It can be used to prepare food or health-care food.

Description

Contain soybean extraction of Yelkin TTS and isoflavones and preparation method thereof
[invention field]
The present invention relates to a kind of plant milk extract, relate to the soybean extraction that contains Yelkin TTS and isoflavones particularly, and this preparation method of extract.
[background technology]
Soybean lecithin is one of nutrient substance that should replenish the every day of U.S. food and the nutrition council (being RDA) recommendation.Be referred to as by medical circle: the basic substance of life, the patron saint of cell, the street cleaner of blood vessel, the food of brain etc.Scientific research has been found that Yelkin TTS is the important component that constitutes cytolemma, and the keeping of existence, activation and organ function of human body cell played an important role.Yelkin TTS is hydrolyzed to glycerine, unsaturated fatty acids, choline and phosphoric acid at human body, and phosphate group wherein is hydrophilic, and fatty acid group is oil loving, these two kinds of groups make Yelkin TTS have emulsifying property, thereby can regulating blood fat, reduce blood cholesterol, the prevention cardiovascular and cerebrovascular diseases; Choline wherein enters that the back generates vagusstoff with intravital acetyl-CoA reaction in the body, and the vagusstoff important transmitter substance that is cerebral nerve, when this material increased in the brain, memory was strengthened, and the brain vigor also obviously improves.Phospholipids content is especially abundant in the soybean, and these phosphatide are compounding mixtures of multiple phosphatide such as Yelkin TTS, kephalin, lipositol, Val, serine phosphatide, sphingomyelin.Wherein the biology of Yelkin TTS phosphatidylcholine by name is called for short PC.The effect of soybean lecithin and function have obtained generally acknowledging of the whole world and have paid close attention to, and its function and preparation method's document and patent have had a large amount of reports and open.
Soybean is the abundant source of isoflavones (daidzein, tri hydroxy isoflavone).Soybean isoflavones is one of main plant class oestrogenic hormon, has weak estrogen-like effects.The estrogenic activity of soybean isoflavones only is 1/100 to 1/1000 of a natural estrogen, plays oestrogenic hormon and antiestrogenic dual regulation effect in vivo, and the women is had health-care effect preferably.Soybean isoflavones also has many biological activitys simultaneously, as: 1) antitumous effect: animal and in vitro tests prove, soybean isoflavones energy arrestin Tyrosylprotein kinase (cell signal changes different key enzyme), but anticancer growth; Dna replication dna be can suppress and necessary topoisomerase one type, two types transcribed; Can suppress vasculogenesis.The adjustable ganglion cell's of isoflavones cell cycle, differentiation and apoptosis.The antitumous effect of isoflavones is in some cancers relevant with hormone, and for example performances such as mammary cancer, the rectum cancer, prostate cancer are more outstanding.2) prevention coronary heart disease: isoflavones also is strong antioxidant, and it is oxidized that particularly the dihydroxy isoflavan can be protected low-density lipoprotein, helps anti-coronary heart disease; 3) preventing osteoporosis: decrease in estrogen, the absorption that influences calcium are that the postmenopausal women sends out major cause well osteoporotic, and the soybean flavonoid has estrogen-like effects, deficiency that can the added body inner estrogen; 4) alleviate or alleviate Woman climacteric symptom menopausal symptoms and also mainly descended by body inner estrogen level and cause, the soybean flavonoid can added body inner estrogen deficiency, improves menopausal symptoms.The soybean flavonoid is as one of the most famous protective foods, and its function and preparation method's document and patent have had a large amount of reports and open.
[summary of the invention]
This project provides a kind of application modern CO 2The mode of supercritical fluid extraction is extracted the extract that contains soybean lecithin and soybean flavonoid simultaneously, and this preparation method of extract is provided.This method has bigger improvement and simplification to the conventional art process route, and quality product improves a lot simultaneously.The present invention realizes above-mentioned purpose with following technical scheme.
Implementation method:
Present method is to utilize supercritical CO 2Abstraction technique directly extracts the novel method that contains Yelkin TTS and isoflavones two class active substances simultaneously in two steps from soyflour.The first step is at extracting pressure 10-25Mpa, under the extraction temperature 30-50 condition, extracts 2 hours, removes soybean oil and steroidal steroid material in the soyflour.Second step was the extraction slag of removing after the soybean oil, add 5% entrainment agent (food grade ethanol, Virahol etc.) circulation 30 minutes earlier, after making the fluid and extract thorough mixing that contains entrainment agent, entrainment agent is added to 10-25%, at extracting pressure 20-35Mpa, under the extraction temperature 40-60 condition, cycling extraction 4-6h, from separating still, emit extract, remove the solvent in the extract, promptly get the soybean extraction that contains Yelkin TTS and isoflavones simultaneously.
Compared with prior art, excellent benefit of the present invention is: do not see the report of producing the soybean extraction that contains two class active substance soybean lecithins and soybean flavonoid simultaneously with method of the present invention in the prior art.Prior art is mainly based on solvent-extraction process, the minority supercritical CO 2The grease in the soybean is also just removed in extraction, and does not directly extract phosphide and isoflavones, and complicated operation, industrial waste go thing many, the foreign matter content height, and final extract poor stability, and two class active substances are separately to prepare.And method service temperature of the present invention is low, can not make phosphatide heat damaging, good product quality; Because supercutical fluid has good mass-transfer performance, so the extraction efficiency height; Entire operation process safety is free from environmental pollution, and by single job, get final product the product soybean extraction, through efficient liquid phase chromatographic analysis, wherein Yelkin TTS (PC) content reaches more than 70%, and total isoflavone content reaches more than 8%, and color and luster is yellowish, no solvent residue, product impurity is few, and the activity substance content height does not contain cholesterol and triglyceride, meeting the demand of current green product development fully, is the good raw material of process for processing food, protective foods.The method for preparing product of the present invention is simple to operate, no trade effluent, waste, and safety, environmental protection have broad application prospects.
Further specify essentiality content of the present invention below in conjunction with test example of the present invention and embodiment, but content of the present invention is not limited thereto.
[test example 1]
One, [experimental technique]
1, raw material: soybean dry powder 8000 grams
2, equipment: 5L X 2 supercritical extraction flow processs are:
Replenish CO 2, add CO 2Basin gets product soybean extraction, CO then through liquefier, high-pressure pump, heat exchanger, extractor, heat exchanger, well heater, separator 2Enter CO through the buffering withdrawer again 2Basin forms circulation.
3, experiment condition: temperature (A) 30-50, (B) 40-60; Pressure: (A) 10-25Mpa, (B) 20-35Mpa; CO 2Flow 50-80L/h;
4, operation steps:
(1) takes by weighing a certain amount of dry soyflour, put into extraction kettle and will cover it and screw.The extraction kettle temperature is made as the 30-50 degree, and pressure is made as 10-25Mpa, and the separating still temperature is made as 50 degree, and pressure is made as 6-7Mpa.
(2) CO is opened in extraction beginning earlier 2Bottle valve makes CO 2Heat to extraction temperature after being frozen into liquid state by gaseous state, again through the high pressure plunger pump compressed fluid to the extracting pressure of setting, enter extraction kettle and contact with material, circulated 2 hours, dissolved the CO of soybean oil 2Fluid enters separating still after the reducing valve pressure release, the CO after the pressure release 2Gas recycles behind spinner-type flowmeter, takes out soybean oil from separator.
(3) remove extraction slag after the soybean oil, at extraction temperature 40-60 degree, pressure 20-35Mpa and separating still temperature 50 degree, under the pressure 6-7Mpa condition, add circulations such as 5% food grade ethanol, Virahol 30 minutes earlier with entrainment agent, after making the fluid and extract thorough mixing that contains entrainment agent, entrainment agent is added to 10-25% circulated 4-6 hour, extract is taken out on circulation limit, limit from separator, extract is spray-dried behind precipitation, can obtain product.
(4) after experiment finishes, close high pressure, with CO 2From separator, recharge in storage tank, standby.
Two, [experimental result]
This experiment has been carried out extraction experiments to six samples and the results are shown in Table 1.From the experimental data of table 1 as can be known: utilize supercritical CO 2Fluid directly extracts phosphatide from soyflour in two steps and isoflavones two class active substances are feasible, its productive rate reaches more than 20% of dried raw material, detect two class active substances in the product, wherein lecithin content up to 70% with, and total isoflavone content reaches more than 8%.It has the product of soybean phospholipid and soybean isoflavones two class active functions simultaneously, is the good raw material of process for processing food, medicine and protective foods.
Active component content table in the soybean extraction of table 1 the inventive method preparation
Experiment numbers Sample weighting amount (g) Productive rate (g) Productive rate (%) Lecithin content % in the product Total isoflavone content % in the product
1 1000 21.65 21.65 70.50 8.5
2 1200 24.06 20.05 71.24 8.9
3 1400 28.21 20.15 70.20 8.7
4 1000 22.05 22.05 69.78 8.8
5 1200 23.37 19.47 71.56 9.1
6 1400 27.45 19.61 70.82 9.3
Mean value＞ - - ＞20 ＞70 ＞8
[embodiment 1]
(1) takes by weighing the dry soyflour of 1000 grams, put into extraction kettle and will cover it and screw.The extraction kettle temperature is set at the 30-50 degree, and pressure is set at 10-25Mpa, and the separating still temperature is set at 50 degree, and pressure is set at 6-7Mpa.
(2) open CO earlier 2Bottle valve makes CO 2Heat to extraction temperature after being frozen into liquid state by gaseous state, again through the high pressure plunger pump compressed fluid to the extracting pressure of setting, enter extraction kettle and contact with material, circulated 2 hours, dissolved the CO of soybean oil 2Fluid enters separating still after the reducing valve pressure release, the CO after the pressure release 2Gas recycles behind spinner-type flowmeter, takes out soybean oil and get 120 grams from separator.
(3) remove extraction slag after the soybean oil, at extraction temperature 40-60 degree, pressure 20-35Mpa and separating still temperature 50 degree, under the pressure 6-7Mpa condition, add circulations such as 5% food grade ethanol, Virahol 30 minutes, after making the fluid and extract thorough mixing that contains entrainment agent, entrainment agent is added to 10-25% circulated 2-3 hour, extract is taken out on circulation limit, limit from separator, the extract film drying that reduces pressure behind precipitation obtains product 21.65 grams.
(4) after experiment finishes, close high pressure, with CO 2From separator, recharge in storage tank, standby.
[embodiment 2]
(1) takes by weighing the dry soyflour of 1200 grams, put into extraction kettle and will cover it and screw.The extraction kettle temperature is set at the 30-50 degree, and pressure is set at 30-25Mpa, and the separating still temperature is set at 50 degree, and pressure is set at 6-7Mpa.
(2) open CO earlier 2Bottle valve makes CO 2Heat to extraction temperature after being frozen into liquid state by gaseous state, again through the high pressure plunger pump compressed fluid to the extracting pressure of setting, enter extraction kettle and contact with material, circulated 2 hours, dissolved the CO of soybean oil 2Fluid enters separating still after the reducing valve pressure release, the CO after the pressure release 2Gas recycles behind spinner-type flowmeter, takes out soybean oil and get 140 grams from separator.
(3) remove extraction slag after the soybean oil, at extraction temperature 40-50 degree, pressure 20-35Mpa and separating still temperature 50 degree, under the pressure 6-7Mpa condition, add circulations such as 5% food grade ethanol, Virahol 30 minutes, after making the fluid and extract thorough mixing that contains entrainment agent, entrainment agent is added to 10-25% circulated 4-6 hour, extract is taken out on circulation limit, limit from separator, the extract film drying that reduces pressure behind precipitation obtains product 24.06 grams.
(4) after experiment finishes, close high pressure, with CO 2From separator, recharge in storage tank, standby.
[embodiment 3]
(1) takes by weighing the dry soyflour of 1400 grams, put into extraction kettle and will cover it and screw.The extraction kettle temperature is set at the 30-50 degree, and pressure is set at 10-25Mpa, and the separating still temperature is set at 50 degree, and pressure is set at 6-7Mpa.
(2) open CO earlier 2Bottle valve makes CO 2Heat to extraction temperature after being frozen into liquid state by gaseous state, again through the high pressure plunger pump compressed fluid to the extracting pressure of setting, enter extraction kettle and contact with material, circulated 2 hours, dissolved the CO of soybean oil 2Fluid enters separating still after the reducing valve pressure release, the CO after the pressure release 2Gas recycles behind spinner-type flowmeter, takes out soybean oil and get 168 grams from separator.
(3) remove extraction slag after the soybean oil, at extraction temperature 40-60 degree, pressure 20-35Mpa and separating still temperature 50 degree, under the pressure 6-7Mpa condition, add circulations such as 5% food grade ethanol, Virahol 30 minutes, after making the fluid and extract thorough mixing that contains entrainment agent, entrainment agent is added to 10-25% circulated 4-6 hour, extract is taken out on circulation limit, limit from separator, the extract film drying that reduces pressure behind precipitation obtains product 28.07 grams.
(4) after experiment finishes, close high pressure, with CO 2From separator, recharge in storage tank, standby.
[embodiment 4]
(1) takes by weighing the dry soyflour of 1000 grams, put into extraction kettle and will cover it and screw.The extraction kettle temperature is set at the 30-50 degree, and pressure is set at 10-25Mpa, and the separating still temperature is set at 50 degree, and pressure is set at 6-7Mpa.
(2) open CO earlier 2Bottle valve makes CO 2Heat to extraction temperature after being frozen into liquid state by gaseous state, again through the high pressure plunger pump compressed fluid to the extracting pressure of setting, enter extraction kettle and contact with material, circulated 2 hours, dissolved the CO of soybean oil 2Fluid enters separating still after the reducing valve pressure release, the CO after the pressure release 2Gas recycles behind spinner-type flowmeter, takes out soybean oil and get 123 grams from separator.
(3) remove extraction slag after the soybean oil, at extraction temperature 40-60 degree, pressure 20-35Mpa and separating still temperature 50 degree, under the pressure 6-7Mpa condition, add circulations such as 5% food grade ethanol, Virahol 30 minutes, after making the fluid and extract thorough mixing that contains entrainment agent, entrainment agent is added to 10-25% circulated 4-6 hour, extract is taken out on circulation limit, limit from separator, the film drying that behind precipitation, reduces pressure, product 22.05 grams.
(4) after experiment finishes, close high pressure, with CO 2From separator, recharge in storage tank, standby.
[embodiment 5]
(1) takes by weighing the dry soyflour of 1200 grams, put into extraction kettle and will cover it and screw.The extraction kettle temperature is set at the 30-50 degree, and pressure is set at 10-25Mpa, and the separating still temperature is set at 50 degree, and pressure is set at 6-7Mpa.
(2) open CO earlier 2Bottle valve makes CO 2Heat to extraction temperature after being frozen into liquid state by gaseous state, again through the high pressure plunger pump compressed fluid to the extracting pressure of setting, enter extraction kettle and contact with material, circulated 2 hours, dissolved the CO of soybean oil 2Fluid enters separating still after the reducing valve pressure release, the CO after the pressure release 2Gas recycles behind spinner-type flowmeter, takes out soybean oil and get 138 grams from separator.
(3) remove extraction slag after the soybean oil, at extraction temperature 40-50 degree, pressure 20-35Mpa and separating still temperature 50 degree, under the pressure 6-7Mpa condition, add circulations such as 5% food grade ethanol, Virahol 30 minutes, after making the fluid and extract thorough mixing that contains entrainment agent, entrainment agent is added to 10-25% circulated 4-6 hour, extract is taken out on circulation limit, limit from separator, the film drying that reduces pressure behind precipitation obtains product 23.37 grams.
(4) after experiment finishes, close high pressure, with CO 2From separator, recharge in storage tank, standby.
[embodiment 6]
(1) takes by weighing the dry soyflour of 1400 grams, put into extraction kettle and will cover it and screw.The extraction kettle temperature is set at the 30-50 degree, and pressure is set at 10-25Mpa, and the separating still temperature is set at 50 degree, and pressure is set at 6-7Mpa.
(2) open CO earlier 2Bottle valve makes CO 2Heat to extraction temperature after being frozen into liquid state by gaseous state, again through the high pressure plunger pump compressed fluid to the extracting pressure of setting, enter extraction kettle and contact with material, circulated 2 hours, dissolved the CO of soybean oil 2Fluid enters separating still after the reducing valve pressure release, the CO after the pressure release 2Gas recycles behind spinner-type flowmeter, takes out soybean oil and get 165 grams from separator.
(3) remove extraction slag after the soybean oil, at extraction temperature 40-50 degree, pressure 20-35Mpa and separating still temperature 50 degree, under the pressure 6-7Mpa condition, add circulations such as 5% food grade ethanol, Virahol 30 minutes, after making the fluid and extract thorough mixing that contains entrainment agent, entrainment agent is added to 10-25% circulated 4-6 hour, extract is taken out on circulation limit, limit from separator, the extract film drying that reduces pressure behind precipitation obtains product 27.45 grams.
(4) after experiment finishes, close high pressure, with CO 2From separator, recharge in storage tank, standby.

Claims (2)

1, the soybean extraction that contains Yelkin TTS and isoflavones is characterized in that with the soybean being the raw material supercritical CO 2Abstraction technique is made as follows: get soybean, at extracting pressure 10-25Mpa, under the extraction temperature 30-50 ℃ of condition, extracted 2 hours, remove soybean oil and steroidal steroid material in the soyflour; Remove the extraction slag after the soybean oil, add 5% food grade ethanol or Virahol entrainment agent circulation 30 minutes earlier, after making the fluid and extract thorough mixing that contains entrainment agent, entrainment agent is added to 10-25%, at extracting pressure 20-35Mpa, under the extraction temperature 40-60 ℃ of condition, cycling extraction 4-6h, from separating still, emit extract, remove the solvent in the extract, promptly get the soybean extraction that contains Yelkin TTS and isoflavones simultaneously.
2, the preparation method who contains the soybean extraction of Yelkin TTS and isoflavones is characterized in that with the soybean being the raw material supercritical CO 2Abstraction technique is undertaken by following step: get soybean, at extracting pressure 10-25Mpa, under the extraction temperature 30-50 ℃ of condition, extracted 2 hours, remove soybean oil and steroidal steroid material in the soyflour; Remove the extraction slag after the soybean oil, add 5% food grade ethanol or Virahol entrainment agent circulation 30 minutes earlier, after making the fluid and extract thorough mixing that contains entrainment agent, entrainment agent is added to 10-25%, at extracting pressure 20-35Mpa, under the extraction temperature 40-60 ℃ of condition, cycling extraction 4-6h, from separating still, emit extract, remove the solvent in the extract, contained the soybean extraction of Yelkin TTS and isoflavones simultaneously.
CNA2005100109984A 2005-08-31 2005-08-31 Soybean extract containing lecithin and isoflavone and its preparation process Pending CN1861592A (en)

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Application Number Priority Date Filing Date Title
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101016312B (en) * 2007-02-07 2011-09-14 崔澎 Method of preparing powder phosphatide and lecithin for medicine and injection from soybean oil residue
CN102977136A (en) * 2012-12-07 2013-03-20 江南大学 Technique for simultaneously extracting soybean lecithin and pigment from soybean oil
CN102972621A (en) * 2012-11-23 2013-03-20 安文娟 Method for preparing residue-free soybean lecithin
CN109254094A (en) * 2018-10-19 2019-01-22 中国检验检疫科学研究院 The method for detecting isoflavone compound

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101016312B (en) * 2007-02-07 2011-09-14 崔澎 Method of preparing powder phosphatide and lecithin for medicine and injection from soybean oil residue
CN102972621A (en) * 2012-11-23 2013-03-20 安文娟 Method for preparing residue-free soybean lecithin
CN102977136A (en) * 2012-12-07 2013-03-20 江南大学 Technique for simultaneously extracting soybean lecithin and pigment from soybean oil
CN109254094A (en) * 2018-10-19 2019-01-22 中国检验检疫科学研究院 The method for detecting isoflavone compound
CN109254094B (en) * 2018-10-19 2021-05-14 中国检验检疫科学研究院 Method for detecting isoflavone compounds

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