CN1830423A - Preparation method of garlicin injection - Google Patents
Preparation method of garlicin injection Download PDFInfo
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- CN1830423A CN1830423A CNA200510051474XA CN200510051474A CN1830423A CN 1830423 A CN1830423 A CN 1830423A CN A200510051474X A CNA200510051474X A CN A200510051474XA CN 200510051474 A CN200510051474 A CN 200510051474A CN 1830423 A CN1830423 A CN 1830423A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
A garlicin injection for preventing and treating acute and chronic bacillary dysentery and enteritis, pertussis, fungus infection of lung and digestive tract, etc is prepared through ultrasonic treating to garlicin and tween-80, mixing with alcohol, ultrafiltration, pouring it in containers containing N2 in dark condition, sterilizing, and lamp examining.
Description
Technical field
The present invention relates to a kind of preparation method of antibacterials garlicin injection, specifically, relating to a kind of is the small-volume injection of main composition with garlicin, garlicin is after the tween 80 supersound process, mix with ethanol, ultrafiltration obtains the garlicin ultrafiltrate again, under the lucifuge condition, prepare, after the supersound process, fill the nitrogen fill, sterilization at last, lamp inspection, packing, make small-volume injection, belong to the chemical preparation field.But this product intravenous drip has broad-spectrum antibacterial action, multiple coccus, bordetella pertussis, diphtheria corynebacterium, dysentery bacterium, typhoid fever and Salmonella paratyphi, escherichia coli, tubercule bacillus etc. is had suppress and lethal effect; Fungal infection there is inhibitory action; Ameba, trichomonas vaginitis, pinworm etc. also there is the inhibition killing action; Have the free radical of removing, antioxidation; Also can blood fat reducing, anticoagulant is prevented and treated atherosclerosis, stops thrombosis; Also have antiviral, the effect of enhancing human body immunity power.Mainly be applicable to deep fungal and bacterial infection, be used for anti-treating acute and chronic bacillary dysentery and enteritis, pertussis, pulmonary and gastral fungal infection, Candida albicans bacteremia, cryptococcal meningitis, pulmonary tuberculosis etc.
Background technology
Bulbus Allii has another name called giant garlic, is the crude drug health food, and is nutritious, contains aminoacid, peptide class, protein, saccharide, fat, inorganic salt, vitamin, Bulbus Allii oil etc., has anti-inflammation, blood pressure lowering, raising immune function of human body.Garlicin is from Bulbus Allii back warp distillation processing, highly concentrates the volatile essential oil that refine is purified, and its main component is an allicin.Have broad-spectrum antibacterial action, multiple coccus, bordetella pertussis, diphtheria corynebacterium, dysentery bacterium, typhoid fever and Salmonella paratyphi, escherichia coli, tubercule bacillus etc. are had suppress and lethal effect; Fungal infection there is inhibitory action; Ameba, trichomonas vaginitis, pinworm etc. also there is the inhibition killing action; Have the free radical of removing, antioxidation; Also can blood fat reducing, anticoagulant is prevented and treated atherosclerosis, stops thrombosis; Also have antiviral, the effect of enhancing human body immunity power.
At present, the garlicin injection on the domestic market has many enterprises to produce, and all adopts traditional handicraft formulated, and the constant product quality of its existence is poor, the medicinal liquid zest is strong, clarity disqualification rate height, and pH value descends, content descends, color burn, and problems such as curative effect instability can't solve all the time.Its main cause is that garlicin partial impurities such as protein, tannin, saccharide, inorganic salt etc. in preparation process do not remove fully, adds the water-fast characteristic of garlicin itself and causes.The breakthrough of this garlicin injection technology has fundamentally solved the problems referred to above.
Summary of the invention
The object of the present invention is to provide a kind of preparation method of garlicin injection.
The garlicin that preparation method of the present invention comprises the steps: to get in the prescription is put in the mortar, adds the tween 80 of recipe quantity, fully grind evenly, and supersound process, standby; Other gets in the prescription ethanol and puts preparation with in the container, and aforementioned mixed liquor is added to accelerate the mode of stirring slowly, stir, after the supersound process, ultrafiltration, ultrafiltrate; Above-mentioned ultrafiltrate is added in the water for injection to accelerate the mode of stirring slowly, after the supersound process, standardize solution, adjust pH, intermediate products through the terminal aseptic filtration, fill the nitrogen fill with medicinal liquid after the assay was approved under the lucifuge condition, sterilization, lamp inspection, packing promptly gets the garlicin injection finished product.
Preparation method detailed process of the present invention is as follows:
A) garlicin of getting in the prescription is put in the mortar, adds the tween 80 of recipe quantity while grinding, fully ground 0.5～2 hour, and supersound process 10～30 minutes, standby.
B) get in the prescription ethanol and put preparation with in the container, above-mentioned mixed liquor is added to accelerate the mode of stirring slowly, stirred 10～30 minutes, supersound process is after 10～30 minutes, is 10,000～30,000 ultrafilter membrane ultrafiltration with molecular cut off, must ultrafiltrate.
C) above-mentioned ultrafiltrate is added water for injection to 70～90% of amount of preparation to accelerate the mode of stirring slowly, after the supersound process 10～30 minutes, standardize solution, adjust pH 3.0～5.5, intermediate products are after the assay was approved, with medicinal liquid after terminal 0.22 μ m filter membrane aseptic filtration, fill the nitrogen fill under the lucifuge condition, sterilization, lamp inspection, packing promptly gets the garlicin injection finished product.
More preferred manufacturing procedure comprises the steps:
A) garlicin of getting in the prescription is put in the mortar, adds the tween 80 of recipe quantity while grinding, fully ground 1 hour, and supersound process 20 minutes, standby.
B) get in the prescription ethanol and put preparation with in the container, above-mentioned mixed liquor is added to accelerate the mode of stirring slowly, stirred 30 minutes, supersound process is after 20 minutes, is 10,000 ultrafilter membrane ultrafiltration with molecular cut off, must ultrafiltrate.
C) above-mentioned ultrafiltrate is added water for injection to 70% of amount of preparation to accelerate the mode of stirring slowly, after the supersound process 20 minutes, standardize solution, adjust pH 3.5～4.5, intermediate products are after the assay was approved, with medicinal liquid after terminal 0.22 μ m filter membrane aseptic filtration, fill the nitrogen fill under the lucifuge condition, sterilization, lamp inspection, packing promptly gets the garlicin injection finished product.
In the above-mentioned preparation method, used solubilizing agent is a tween 80; Used cosolvent is an ethanol.
In the above-mentioned preparation method, the addition sequence of garlicin and tween 80 adds tween 80 during formulated product after adding garlicin earlier; Milling time is 0.5～2 hour.
In the above-mentioned preparation method, adopting the molecular weight that dams is 10,000 ultrafilter membrane ultrafiltration, is the controlled step of invalid components in institute's preparating liquid, and used ultrafilter membrane is the commercially available prod.Its manufacturer should be the production unit of industry approval.
In the above-mentioned preparation method, lucifuge, logical condition of nitrogen gas are the committed steps of medicinal liquid preparation, pouring process.
In the above-mentioned preparation method, the employed filter membrane of terminal aseptic filtration aperture is 0.22 μ m.For making constant product quality, filter membrane should be as far as possible by fixedly manufacturer's supply.
In the above-mentioned preparation method, the time of supersound process is 10～30 minutes during formulated product, and this step is that garlicin dissolves uniform key technology in water for injection.
In the above-mentioned preparation method, for making constant product quality, used pH value regulator is 10% hydrochloric acid solution, and pH value is strict controlled in 3.5～4.5.
Intravenous drip during garlicin injection clinical practice of the present invention, a 60～120mg, the child is cut down according to the circumstance,
Be diluted in 5～10% glucoses or Dextrose and Sodium Chloride Inj. of 500～1000ml, slowly instil 1 time on the one.
Garlicin injection of the present invention has broad-spectrum antibacterial action, multiple coccus, bordetella pertussis, diphtheria corynebacterium, dysentery bacterium, typhoid fever and Salmonella paratyphi, escherichia coli, tubercule bacillus etc. is had suppress and lethal effect; Fungal infection there is inhibitory action; Ameba, trichomonas vaginitis, pinworm etc. also there is the inhibition killing action; Have the free radical of removing, antioxidation; Also can blood fat reducing, anticoagulant is prevented and treated atherosclerosis, stops thrombosis; Also have antiviral, the effect of enhancing human body immunity power.Mainly be applicable to deep fungal and bacterial infection, be used for anti-treating acute and chronic bacillary dysentery and enteritis, pertussis, pulmonary and gastral fungal infection, Candida albicans bacteremia, cryptococcal meningitis, pulmonary tuberculosis etc.
Garlicin is handled through ultrasonic, hyperfiltration technique among the present invention; and lucifuge, nitrogen filled protection arranged; guaranteed garlicin mix homogeneously in water; invalid element is removed fully, has avoided the degraded of garlicin, has reduced the related substance in the preparation; make the better stability of preparation of making; zest is little, and clarity is improved, stable curative effect.
The specific embodiment
Further describe the present invention with embodiment below, but described embodiment only is used to illustrate the present invention rather than restriction the present invention.
Embodiment 1
A) garlicin of getting in the prescription is put in the mortar, adds the tween 80 of recipe quantity while grinding, fully ground 2 hours, and supersound process 10 minutes, standby.
B) get in the prescription ethanol and put preparation with in the container, above-mentioned mixed liquor is added to accelerate the mode of stirring slowly, stirred 10 minutes, supersound process is after 30 minutes, is 30,000 ultrafilter membrane ultrafiltration with molecular cut off, must ultrafiltrate.
C) above-mentioned ultrafiltrate is added water for injection to 90% of amount of preparation to accelerate the mode of stirring slowly, after the supersound process 30 minutes, standardize solution, adjust pH 4.0, intermediate products are after the assay was approved, with medicinal liquid after terminal 0.22 μ m filter membrane aseptic filtration, fill the nitrogen fill under the lucifuge condition, sterilization, lamp inspection, packing promptly gets the garlicin injection finished product.
Embodiment 2
A) garlicin of getting in the prescription is put in the mortar, adds the tween 80 of recipe quantity while grinding, fully ground 0.5 hour, and supersound process 30 minutes, standby.
B) get in the prescription ethanol and put preparation with in the container, above-mentioned mixed liquor is added to accelerate the mode of stirring slowly, stirred 20 minutes, supersound process is after 10 minutes, is 20,000 ultrafilter membrane ultrafiltration with molecular cut off, must ultrafiltrate.
C) above-mentioned ultrafiltrate is added water for injection to 80% of amount of preparation to accelerate the mode of stirring slowly, after the supersound process 10 minutes, standardize solution, adjust pH 5.0, intermediate products are after the assay was approved, with medicinal liquid after terminal 0.22 μ m filter membrane aseptic filtration, fill the nitrogen fill under the lucifuge condition, sterilization, lamp inspection, packing promptly gets the garlicin injection finished product.
Embodiment 3
A) garlicin of getting in the prescription is put in the mortar, adds the tween 80 of recipe quantity while grinding, fully ground 1.5 hours, and supersound process 15 minutes, standby.
B) get in the prescription ethanol and put preparation with in the container, above-mentioned mixed liquor is added to accelerate the mode of stirring slowly, stirred 25 minutes, supersound process is after 15 minutes, is 10,000 ultrafilter membrane ultrafiltration with molecular cut off, must ultrafiltrate.
C) above-mentioned ultrafiltrate is added water for injection to 75% of amount of preparation to accelerate the mode of stirring slowly, after the supersound process 25 minutes, standardize solution, adjust pH 3.0, intermediate products are after the assay was approved, with medicinal liquid after terminal 0.22 μ m filter membrane aseptic filtration, fill the nitrogen fill under the lucifuge condition, sterilization, lamp inspection, packing promptly gets the garlicin injection finished product.
Embodiment 4
A) garlicin of getting in the prescription is put in the mortar, adds the tween 80 of recipe quantity while grinding, fully ground 0.75 hour, and supersound process 25 minutes, standby.
B) get in the prescription ethanol and put preparation with in the container, above-mentioned mixed liquor is added to accelerate the mode of stirring slowly, stirred 15 minutes, supersound process is after 25 minutes, is 30,000 ultrafilter membrane ultrafiltration with molecular cut off, must ultrafiltrate.
C) above-mentioned ultrafiltrate is added water for injection to 85% of amount of preparation to accelerate the mode of stirring slowly, after the supersound process 15 minutes, standardize solution, adjust pH 5.5, intermediate products are after the assay was approved, with medicinal liquid after terminal 0.22 μ m filter membrane aseptic filtration, fill the nitrogen fill under the lucifuge condition, sterilization, lamp inspection, packing promptly gets the garlicin injection finished product.
Embodiment 5
A) garlicin of getting in the prescription is put in the mortar, adds the tween 80 of recipe quantity while grinding, fully ground 2 hours, and supersound process 15 minutes, standby.
B) get in the prescription ethanol and put preparation with in the container, above-mentioned mixed liquor is added to accelerate the mode of stirring slowly, stirred 20 minutes, supersound process is after 30 minutes, is 20,000 ultrafilter membrane ultrafiltration with molecular cut off, must ultrafiltrate.
C) above-mentioned ultrafiltrate is added water for injection to 80% of amount of preparation to accelerate the mode of stirring slowly, after the supersound process 25 minutes, standardize solution, adjust pH 4.5, intermediate products are after the assay was approved, with medicinal liquid after terminal 0.22 μ m filter membrane aseptic filtration, fill the nitrogen fill under the lucifuge condition, sterilization, lamp inspection, packing promptly gets the garlicin injection finished product.
Experimental example 1
The detection of relevant every gainer under character, pH value and the injection item of this experimental example for the prepared garlicin injection of the present invention.
Character: this product is yellowish clear liquid, and the tool garlic odour is up to specification.
PH value: get this product, measure (two appendix VIH of Chinese Pharmacopoeia version in 2000) in accordance with the law, pH value is 3.0～5.5, and is up to specification.
Other: meet every regulation relevant under the injection item (two appendix I of Chinese Pharmacopoeia version in 2000 B).
Experimental example 2
This experimental example is the qualitative determination of the prepared garlicin injection of the present invention.
Differentiate: getting this product 1ml, to add dehydrated alcohol 1ml mixed, adds 2 of sodium sulfide test solutions, is yellow, up to specification.
Contain definite component in the prepared garlicin injection of above description of test the present invention.
Experimental example 3
This experimental example is the assay of the prepared garlicin injection of the present invention.
Assay: measure according to gas chromatography (two appendix VE of Chinese Pharmacopoeia version in 2000).Chromatographic condition and system suitability test are immobile phase with the ethylene glycol adipate, and coating concentration is 0.5%, and column temperature is 60 ℃, and detector is a flame ionization ditector, and the separating degree of garlicin peak and internal standard substance mass peak should meet the requirements.It is an amount of that correction factor mensuration is got 1-Phenylethanone..Add n-hexane dissolution and dilution, make the solution that contains 10mg among every 1ml, as inner mark solution.
Other gets the about 30mg of garlicin reference substance, and accurate the title decides, and puts in the 10ml volumetric flask, and the accurate inner mark solution 1ml that adds adds normal hexane and is diluted to scale, shakes up, and gets 1～2 μ l inject gas chromatograph, the calculation correction factor.The accurate this product an amount of (being equivalent to garlicin 30mg approximately) of drawing of algoscopy with normal hexane shaking out 3 times (each 2ml), is told the normal hexane layer, puts in the 10ml volumetric flask.The accurate inner mark solution 1ml that adds adds normal hexane and is diluted to scale, shakes up, and leaves standstill in 15 minutes, gets supernatant 1～2 μ l inject gas chromatograph, measures, and calculates.
Standard code: contain garlicin (C6H10S3) and should be less than 80.0% of labelled amount.
Assay by three batches the results are shown in following table:
| Lot number | Labelled amount (%) |
| 20040301 | 98.9 |
| 20040302 | 100.6 |
| 20040303 | 99.3 |
Experimental example 4
This experimental example is the security inspection of the prepared garlicin injection of the present invention.
Aseptic: get this product, check (two appendix XIH of Chinese Pharmacopoeia version in 2000) in accordance with the law, up to specification.
Bacterial endotoxin: get this product, check (two appendix XIE of Chinese Pharmacopoeia version in 2000) in accordance with the law, every 1ml contains the bacterial endotoxin amount less than 1EU, and is up to specification.
Long term toxicity is measured: successive administration was observed the hematuria inspection in three months, pathologic finding there is no unusually.
Safety by the prepared garlicin injection of above description of test the present invention meets the injection requirement, does not have any toxic action, uses human body safety.
Comparative example 1
The clarity of the garlicin injection that this comparative example explanation the present invention is prepared is better than producing with conventional preparation method.
The clarity criterion:
(1) finds no foreign body or only be with micro-white point person, the lattice theory of making a match.
(2) every bottle contains the hair that is shorter than 0.5cm and the white point of 0.1～0.2mm, white piece or color dot sum above 5 persons, the lattice theory of making a match.
(3) disqualification rate surpasses 5%, this batch product lattice theory of making a match.
Relevant notion:
White piece: mean with the inspection method of regulation, can see the whiteness that tangible plane or corner angle are arranged.
White point: can not differentiate plane or corner angle by white point.
Trace white point: in official hour, only see 3 or 3 following white point persons.
Foreign body: comprise chips of glass, fiber, color dot, color lump and other external foreign body.
Inspection method:
Get respectively and adopt prepared garlicin injection of the present invention and each 200 of the products that adopts the common process preparation,, the results are shown in following table by " clarity test detailed rules and regulations and criterion " and above standard inspection.
| Group | The trace white point | White point | White piece | Foreign body | Number of non-compliances | Disqualification rate | Qualification rate | Conclusion |
| The invention group | 7 | 1 surpasses 5 | 0 | 0 | 1 | 0.5％ | 99.5％ | Qualified |
| Conventional group | 70 | 8 surpass 5 | 2 surpass 5 | 0 | 10 | 5％ | 95％ | Qualified |
Clarity by the prepared garlicin injection of above description of test the present invention obviously is better than producing with conventional preparation method.
Comparative example 2
This comparative example illustrates that the prepared garlicin injection zest when clinical practice of the present invention is less.
| Group | The invention group | Conventional group |
| Usage and dosage | Intravenous drip, a 60～120mg, the child is cut down according to the circumstance, be diluted in 5～10% glucoses or Dextrose and Sodium Chloride Inj. of 500～1000ml, slowly instil 1 time on the one. | Intravenous drip, a 60～120mg, the child is cut down according to the circumstance, be diluted in 5～10% glucoses or Dextrose and Sodium Chloride Inj. of 500～1000ml, slowly instil 1 time on the one. |
| Clinical practice | Treat 56 routine fungus and bacterial infections | Treat 51 routine fungus and bacterial infections |
| Zest is observed | No pain and untoward reaction phenomenon | The injection site is all rubescent, and pain and burning sensation appear in 26 examples. |
Comparative example 3
The prepared garlicin injection of this comparative example explanation employing the present invention compares with the stability of the garlicin injection that adopts conventional preparation method to produce by six months accelerated tests, the results are shown in following table.
Standard code:
Content: this product contains garlicin (C
6H
10S
3) should be less than 80.0% of labelled amount.
PH value: should be 3.0～5.5
Clarity: qualification rate should be not less than 95%
Color: should be yellowish clear liquid
By above experiment as can be seen, the invention group was passed through accelerated tests after six months, significant change does not take place in its content, pH value, clarity, color, and the content pH value of conventional group all has obvious reduction, clarity, color are all against regulation, show that the prepared garlicin injection of the present invention obviously is better than adopting conventional preparation method to be produced.
Claims (10)
1. the preparation method of an antibacterials garlicin injection, described preparation method detailed process is as follows:
A) garlicin of getting in the prescription is put in the mortar, adds the tween 80 of recipe quantity, fully grind evenly, and supersound process, standby;
B) get in the prescription ethanol and put preparation, above-mentioned mixed liquor is added to accelerate the mode of stirring slowly, stir with in the container, after the supersound process, ultrafiltration, ultrafiltrate;
C) above-mentioned ultrafiltrate is added in the water for injection to accelerate the mode of stirring slowly, after the supersound process, standardize solution, adjust pH, intermediate products after the terminal aseptic filtration, fill the nitrogen fill with medicinal liquid after the assay was approved under the lucifuge condition, sterilization, lamp inspection, packing promptly gets the garlicin injection finished product.
2. according to claims 1 described preparation method, it is characterized in that comprising the steps:
A) garlicin of getting in the prescription is put in the mortar, adds the tween 80 of recipe quantity while grinding, fully ground 0.5～2 hour, and supersound process 10～30 minutes, standby;
B) get in the prescription ethanol and put preparation with in the container, above-mentioned mixed liquor is added to accelerate the mode of stirring slowly, stirred 10～30 minutes, supersound process is after 10～30 minutes, is 10,000～30,000 ultrafilter membrane ultrafiltration with molecular cut off, must ultrafiltrate;
C) above-mentioned ultrafiltrate is added water for injection to 70～90% of amount of preparation to accelerate the mode of stirring slowly, after the supersound process 10～30 minutes, standardize solution, adjust pH 3.0～5.5, intermediate products are after the assay was approved, with medicinal liquid after terminal 0.22 μ m filter membrane aseptic filtration, fill the nitrogen fill under the lucifuge condition, sterilization, lamp inspection, packing promptly gets the garlicin injection finished product.
3. according to claims 1 described preparation method, it is characterized in that comprising the steps:
A) garlicin of getting in the prescription is put in the mortar, adds the tween 80 of recipe quantity while grinding, fully ground 1 hour, and supersound process 20 minutes, standby;
B) get in the prescription ethanol and put preparation with in the container, above-mentioned mixed liquor is added to accelerate the mode of stirring slowly, stirred 30 minutes, supersound process is after 20 minutes, is 10,000 ultrafilter membrane ultrafiltration with molecular cut off, must ultrafiltrate;
C) above-mentioned ultrafiltrate is added water for injection to 70% of amount of preparation to accelerate the mode of stirring slowly, after the supersound process 20 minutes, standardize solution, adjust pH 4.0～5.0, intermediate products are after the assay was approved, with medicinal liquid after terminal 0.22 μ m filter membrane aseptic filtration, fill the nitrogen fill under the lucifuge condition, sterilization, lamp inspection, packing promptly gets the garlicin injection finished product.
4. according to claims 1,2 or 3 described preparation methoies, the addition sequence of garlicin and tween 80 adds tween 80 when it is characterized in that formulated product after adding garlicin earlier; Milling time is 0.5～2 hour.
5. according to claims 1,2 or 3 described preparation methoies, the time of supersound process is 10～30 minutes when it is characterized in that formulated product.
6. according to claims 1,2 or 3 described preparation methoies, it is characterized in that the employed ultrafilter membrane of ultrafiltration is that molecular cut off is 10,000～30,000 ultrafilter membrane.
7. according to claims 1,2 or 3 described preparation methoies, it is characterized in that preparing, the condition in the pouring process is logical nitrogen, lucifuge.
8. according to claims 1,2 or 3 described preparation methoies, it is characterized in that used pH value regulator is 10% hydrochloric acid solution, pH value is controlled at 3.5～4.5.
9. according to claims 1,2 or 3 described preparation methoies, it is characterized in that adding in the described step (C) water for injection to 70～90% of amount of preparation.
10. according to claims 1,2 or 3 described preparation methoies, it is characterized in that used solubilizing agent is a tween 80; Used cosolvent is an ethanol.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA200510051474XA CN1830423A (en) | 2005-03-08 | 2005-03-08 | Preparation method of garlicin injection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA200510051474XA CN1830423A (en) | 2005-03-08 | 2005-03-08 | Preparation method of garlicin injection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1830423A true CN1830423A (en) | 2006-09-13 |
Family
ID=36993025
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA200510051474XA Pending CN1830423A (en) | 2005-03-08 | 2005-03-08 | Preparation method of garlicin injection |
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| Country | Link |
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| CN (1) | CN1830423A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011160425A1 (en) * | 2010-06-22 | 2011-12-29 | 新疆埃乐欣药业有限公司 | Preparing method of allicin injection and low temperature continuous stirring ultrafiltration device thereof |
-
2005
- 2005-03-08 CN CNA200510051474XA patent/CN1830423A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011160425A1 (en) * | 2010-06-22 | 2011-12-29 | 新疆埃乐欣药业有限公司 | Preparing method of allicin injection and low temperature continuous stirring ultrafiltration device thereof |
| US9023623B2 (en) | 2010-06-22 | 2015-05-05 | Xinjiang Ailexin Pharmaceutical Co., Ltd. | Method for preparing of allicin injection and low-temperature continuous stirring ultrafiltration device thereof |
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