CN1732770A - Method for improving variety of hard clam - Google Patents

Method for improving variety of hard clam Download PDF

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Publication number
CN1732770A
CN1732770A CN 200510050583 CN200510050583A CN1732770A CN 1732770 A CN1732770 A CN 1732770A CN 200510050583 CN200510050583 CN 200510050583 CN 200510050583 A CN200510050583 A CN 200510050583A CN 1732770 A CN1732770 A CN 1732770A
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China
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clam
different
clams
ovum
shellfish
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CN 200510050583
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CN100418413C (en
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李太武
苏秀榕
张安国
刘保忠
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Ningbo University
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Ningbo University
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Abstract

The invention discloses a method of improving clam breed, charactering in the following steps: i.selecting calms from the same or different marine site, feeding temporally calms with the same or different pattern. ii.selecting male and female individual clams with mature gonad to hasten producing. iii.observing the situation of said individual discharging ovum and sperm, mixing right amount ovum and sperms fully to make impregnations after they discharging completely. iv.putting zygotes to seawater to proceed hatching feeding, then gaining improved young clams. It is characterized in making use of crossbreeding new technique to improve clam breed, culture new varietals of clams, making use of the feature that clams from different geo-groups are significant of difference to crossbreed clams from different marine site, also clams with different pattern, culturing excellent breeds with high speed of growth, high rate of surviving, great capability against contrary, uniform pattern, and the clams produced by crossbreeding grow 3-5 times faster than the ones by auto copulation.

Description

A kind of method that improves variety of hard clam
Technical field
The present invention relates to a kind of new technology in a kind of artificial breeding of seashells, especially relate to a kind of method that improves variety of hard clam.
Background technology
Clam (Meretrix meretrix Linnaeus) belongs to Mollusca, Bivalvia, Eulamellibranchia, Veneridae, Meretrix, is the coastal common a kind of important economic shellfish of China.Wherein near the Liaohe River mouth in Liaoning Province, the West Coast one band clam resource in the Yangjiang in the Bei Haiwan in coastal, the Guangxi of the Laizhou Wan of Shandong Province, South Jiangsu, Guangdong and Taiwan is particularly abundant.Clam fine and tender taste, nutritious, plain celebrated with " the first under heaven is bright ", and it is nutritious, every hectogram meat contains protein 13.9 grams, fat 0.8 gram, also containing the mineral matter of multiple needed by human such as easy each seed amino acid that absorbs of human body and vitamin and calcium, potassium, magnesium, phosphorus, iron, once was the precious tribute in imperial palace sea during the Tang Dynasty.Clam is delicious meat, nutritious not only, and has very high dietotherapy medical value, and Compendium of Material Medica claims it to control " sore, furuncle poison, the long-pending piece that disappears, relieving alcoholism " waits disease.Recent studies shows again, and clam has clearing heat and promoting diuresis, reduces phlegm, the effect of dissipating bind, and liver cancer is had the obvious suppression effect, and diseases such as asthma, chronic bronchitis, thyromegaly, scrofula are also had obvious curative effects.Edible clam has moisturizing viscera, only quenches one's thirst, and strengthening the spleen and stomach is controlled Akamu, increases emulsion and function such as antitumor.Except that eating raw, also can be processed into dry product and tinned food.The clam that lives all is the products of exporting goods and earning foreign currency with freezing bright clam meat.
As far back as fishing and hunting period, clam just begins to be adopted predation by the mankind and uses; According to the record of relevant document, the period Tang Dynasty as offerings; Along with the continuous growth of international market to the clam demand, the cultivating clam of China has already obtained developing rapidly.In recent years, the cultivating clam of the coastal each department of China already develops rapidly.Southern to the North Sea, Guangxi, north is to various places, Liaoning, and cultivating clam has become the pillar industry that rural area well-to-do level and modernization are quickened to realize in the coastal fishing zone of China.According to incompletely statistics, surplus national cultivating clam area has reached 14 ten thousand mu, about 60,000 tons of annual productions, export volume reaches 20,000 tons.Cultivating clam has greatly promoted the development of this industry with its less investment, instant effect, profitable, advantage such as technical management is convenient.But the required seed overwhelming majority of clam aquaculture catches wild seed from adopting.In the last few years, because factors such as overfishing and environmental deterioration cause clam natural resources amount seriously to reduce, clam nature seed resource is rich owed unstable, was seriously restricting the sound development of cultivating clam industry.For accelerating the recovery of natural resources, and the development of promotion cultivating clam industry, development " the extensive artificial breeding technique of clam " is imperative, but in the process of finishing the clam artificial breeding, the resistance that it is found that the clam juvenile mollusk is very poor, lethality is high, and having healthy and strong juvenile mollusk then is the prerequisite that cultivating clam already develops.So, how to utilize the modern biology technology to carry out breed improvement, the new varieties of cultivating strong stress resistance, fast growth have been the task of top priority.
Chinese patent application CN02133009.3 discloses a kind of clam and has grown seedlings and survive the winter and method is bred in the centre, this method to clam parent shellfish cultivate, clam kind shellfish lays eggs, hatch cultivation and juvenile mollusk is survived the winter, breed and proposed to bury the shellfish accelerating breeding method of dwelling, fundamentally solved the pond because of substrate pollution, the difficult problem of death by suffocation is caused in the latent husky back of bait feeding and juvenile mollusk because of a large amount of secreting mucus; This invention has also proposed with strong current the juvenile mollusk itself and the layer of sand purging method of depending on for existence in addition, thereby makes juvenile mollusk keep higher survival rate; This invention has also proposed to use during juvenile mollusk is cultured in the winter time winter that shrimp pond construction of platform is put net cage to survive the winter and has bred method, solved the northern area juvenile mollusk difficult problem of surviving the winter, make kind of the shellfish egg-laying period one month earlier, realized that clam lays eggs then to growing seedlings, the juvenile mollusk overwintering survival rate reaches 90%, and intermediate rearing reaches 69.6%, can reach 90 millimeters through cultivating into the clam diameter, weigh about 100 grams, this invention has been started new way for cultivating clam at north cold area.
But this method mainly is to improve the young shellfish survival rate of clam, still can not be from key issues such as basic solution clam juvenile mollusk growth rate and survival rates.
In addition, the surface of the clam of the coastal different waters of China difference is pattern, and the clam decorative pattern of Huang Bohai is more and beautiful, the clam color at the East Sea and the South Sea is single to have decorative pattern hardly, as shown in Figure 5, decorative pattern is just arranged, just do not have decorative pattern if take from Jiangsu if Zhejiang clam seedling is taken from Shandong.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of method that improves variety of hard clam at above-mentioned prior art present situation, by improvement to variety of hard clam, cultivate the juvenile mollusk fast growth, the clam new varieties that survival rate is high, and cultivate the beautiful and consistent clam new varieties of decorative pattern.
The present invention solves the problems of the technologies described above the technical scheme that is adopted: a kind of method that improves variety of hard clam is characterized in that it may further comprise the steps: 1. select the clam of zones of different to carry out supporting temporarily; 2. selecting the male and female parent shellfish individuality of the zones of different of gonadal maturation hastens parturition; 3. observe close shellfish and discharge the situation of smart ovum, treat that close shellfish has been arranged smart ovum after, an amount of sperm and the abundant mixing of ovum are made its fertilization; 4. fertilized egg is put into seawater and hatch cultivation, the juvenile mollusk after obtaining improveing.
In the zones of different of step in 1. can be different marine sites, also can be the different area in identical marine site.
In the concrete operations step of step in 2. be: at first the clam parent shellfish individuality of gonadal maturation is carried out dry in the shade stimulation at least hour, put it into then in the beaker that fills 30 ℃ of left and right sides seawater, adopt the abductive approach of heat up stimulation or different in nature product to impel the smart ovum of its output simultaneously.
2. can be genetic marker with the decorative pattern further in step in, selects the male and female parent shellfish individuality of different decorative patterns and hastens parturition.
Compared with prior art, the invention has the advantages that and be to utilize the crossbreeding new technology to improve variety of hard clam, utilize remarkable this feature of clam difference of different geographical populations, with the different geographical environments in each marine site clam hybridization, the juvenile mollusk fast growth of cultivating, survival rate height, strong stress resistance are the excellent strain of clam; As depicted in figs. 1 and 2, the clam offspring growth rate of the clam offspring's of hybridization growth rate and same place clam selfing improves 3-5 doubly; Selecting the clam hybridization of different decorative patterns, is the hereditary separation characteristic according to the clam offspring, is that genetic marker is carried out breed improvement with the decorative pattern, can further filter out decorative pattern on all four good the clam new varieties and the new lines of offspring's shell.As shown in Figure 3 and Figure 4, the decorative pattern of the clam offspring's of hybridization shell can be in full accord according to people's design, and the clam offspring's of selfing growth rate is relatively slow.
Description of drawings
Fig. 1 is the clam offspring's of the present invention's hybridization growing state figure;
Fig. 2 is the clam offspring's of selfing growing state figure;
The decorative pattern picture of clam parent that Fig. 3 is used to hybridize for the present invention and the shell of filial generation;
Fig. 4 is the decorative pattern picture of the clam parent of selfing and self progeny's shell;
Fig. 5 is the clam parent's of various places the decorative pattern picture of shell.
Embodiment
Below in conjunction with embodiment the present invention is described in further detail.
Embodiment one: a kind of method that improves variety of hard clam, and it may further comprise the steps: 1. select the dark dapple close again clam male and female parent shellfish individuality of the North Sea, Guangxi and Nantong to carry out supporting temporarily, shell is long on average greater than 25mm, and the shell height is on average greater than 23mm; 2. the clam parent shellfish individuality with gonadal maturation carried out dry in the shade stimulation 1 hour, put it into then in the beaker that fills greater than 27 ℃ of left and right sides seawater, and the abductive approach that adopting simultaneously heats up stimulates impels the smart ovum of its output; 3. observe close shellfish and discharge the situation of smart ovum, treat that close shellfish has been arranged smart ovum after, an amount of sperm and the abundant mixing of ovum are made its fertilization; 4. fertilized egg is put into seawater and hatch cultivation, the juvenile mollusk after obtaining improveing.
Embodiment two: a kind of method that improves variety of hard clam, and it may further comprise the steps: 1. select the light dapple close again clam male and female parent shellfish individuality in the Huanghai Sea and the East Sea to carry out supporting temporarily, shell is long on average greater than 25mm, and the shell height is on average greater than 20mm; 2. the clam parent shellfish individuality with gonadal maturation carried out dry in the shade stimulation 2 hours, put it into then in the beaker that fills greater than 26 ℃ of left and right sides seawater, adopted the abductive approach of different in nature product to impel the smart ovum of its output simultaneously; 3. observe close shellfish and discharge the situation of smart ovum, treat that close shellfish has been arranged smart ovum after, an amount of sperm and the abundant mixing of ovum are made its fertilization; 4. fertilized egg is put into seawater and hatch cultivation, the juvenile mollusk after obtaining improveing.
Embodiment three: a kind of method that improves variety of hard clam, and it may further comprise the steps: 1. select the dark dapple close again clam male and female parent shellfish individuality of Liaoning Panjin and Laizhou, Shandong to carry out supporting temporarily, shell is long on average greater than 40mm, and the shell height is on average greater than 30mm; 2. the clam parent shellfish individuality with gonadal maturation carried out dry in the shade stimulation 1 hour 30 minutes, put it into then in the beaker that fills greater than 25 ℃ of left and right sides seawater, and the abductive approach that adopting simultaneously heats up stimulates impels the smart ovum of its output; 3. observe close shellfish and discharge the situation of smart ovum, treat that close shellfish has been arranged smart ovum after, an amount of sperm and the abundant mixing of ovum are made its fertilization; 4. fertilized egg is put into seawater and hatch cultivation, the juvenile mollusk after obtaining improveing.
Embodiment four: a kind of method that improves variety of hard clam, and it may further comprise the steps: 1. select the dark decorative pattern clam male and female parent shellfish individuality of shallow decorative pattern in the South Sea and Bo Hai to carry out supporting temporarily, the long average out to of shell is greater than 32mm, the high average out to 30mm of shell; 2. the clam parent shellfish individuality with gonadal maturation carried out dry in the shade stimulation 1 hour 20 minutes, put it into then in the beaker that fills greater than 29 ℃ of left and right sides seawater, adopted the abductive approach of different in nature product to impel the smart ovum of its output simultaneously; 3. observe close shellfish and discharge the situation of smart ovum, treat that close shellfish has been arranged smart ovum after, an amount of sperm and the abundant mixing of ovum are made its fertilization; 4. fertilized egg is put into seawater and hatch cultivation, the juvenile mollusk after obtaining improveing.2. is genetic marker with the decorative pattern in step in, selects the male and female parent shellfish individuality of different decorative patterns and hastens parturition.
Embodiment five: a kind of method that improves variety of hard clam, and it may further comprise the steps: 1. select the Zhejiang clam male and female parent shellfish individuality of the clam of the shallow decorative pattern of the Huanghai Sea and no decorative pattern that seedling is taken from Jiangsu to carry out supporting temporarily, the long average out to of shell is greater than 40mm, the high average out to 30mm of shell; 2. the clam parent shellfish individuality with gonadal maturation carried out dry in the shade stimulation 2 hours 30 minutes, put it into then in the beaker that fills 30 ℃ of left and right sides seawater, and the abductive approach that adopting simultaneously heats up stimulates impels the smart ovum of its output; 3. observe close shellfish and discharge the situation of smart ovum, treat that close shellfish has been arranged smart ovum after, an amount of sperm and the abundant mixing of ovum are made its fertilization; 4. fertilized egg is put into seawater and hatch cultivation, the juvenile mollusk after obtaining improveing.
Embodiment six: a kind of method that improves variety of hard clam, and it may further comprise the steps: 1. select the shallow decorative pattern clam male and female parent shellfish individuality of the dark decorative pattern clam of Nantong and Laizhou, Shandong to carry out supporting temporarily, the long average out to of shell is greater than 34mm, the high average out to 31mm of shell; 2. the clam parent shellfish individuality with gonadal maturation carried out dry in the shade stimulation 3 hours, put it into then in the beaker that fills 29 ℃ of left and right sides seawater, adopted the abductive approach of different in nature product to impel the smart ovum of its output simultaneously; 3. observe close shellfish and discharge the situation of smart ovum, treat that close shellfish has been arranged smart ovum after, an amount of sperm and the abundant mixing of ovum are made its fertilization; 4. fertilized egg is put into seawater and hatch cultivation, the juvenile mollusk after obtaining improveing.

Claims (5)

1, a kind of method that improves variety of hard clam is characterized in that it may further comprise the steps: 1. select the clam of the clam of zones of different, different decorative patterns to carry out supporting temporarily; 2. selecting the male and female parent shellfish individuality of gonadal maturation hastens parturition; 3. observe close shellfish and discharge the situation of smart ovum, treat that close shellfish has been arranged smart ovum after, an amount of sperm and the abundant mixing of ovum are made its fertilization; 4. fertilized egg is put into seawater and hatch cultivation, the juvenile mollusk after obtaining improveing.
2, a kind of method that improves variety of hard clam as claimed in claim 1 is characterized in that in the zones of different of step in 1. be different marine sites.
3, a kind of method that improves variety of hard clam as claimed in claim 1 is characterized in that in the zones of different of step in 1. be the different area in identical marine site.
4, a kind of method that improves variety of hard clam as claimed in claim 1, it is characterized in that at first the clam of gonadal maturation parent shellfish individuality being carried out dry in the shade stimulation at least 1 hour in 2. in step, put it into then and fill greater than in 25 ℃ of left and right sides seawater, adopting simultaneously heats up stimulates or the abductive approach of different in nature product impels the smart ovum of its output.
5, a kind of method that improves variety of hard clam as claimed in claim 1 is characterized in that be genetic marker with the decorative pattern in step in 2., selects the male and female parent shellfish individuality of the identical or different decorative pattern of different regions, areal and hastens parturition.
CNB200510050583XA 2005-07-01 2005-07-01 Method for improving variety of hard clam Expired - Fee Related CN100418413C (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101926292B (en) * 2009-06-25 2013-01-09 浙江万里学院 Method for breeding tiger-stripe meretrix linnaeus strains
CN101926293B (en) * 2009-06-25 2013-09-18 浙江万里学院 Breeding method of clam fast growth strain
CN106804492A (en) * 2017-01-19 2017-06-09 中国海洋大学 The measuring method of one seed oyster parent's shellfish gonad maturity and application
CN114642191A (en) * 2021-12-08 2022-06-21 连云港市农业科学院 Method for hybridization between different bean hawkmoth geographic populations

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1180687C (en) * 2001-08-03 2004-12-22 中国科学院海洋研究所 Selective cross breeding method of shellfishes
CN1268202C (en) * 2002-08-14 2006-08-09 辽宁省海洋水产研究所 Hybridization technique between different kinds of sea urchin

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101926292B (en) * 2009-06-25 2013-01-09 浙江万里学院 Method for breeding tiger-stripe meretrix linnaeus strains
CN101926293B (en) * 2009-06-25 2013-09-18 浙江万里学院 Breeding method of clam fast growth strain
CN106804492A (en) * 2017-01-19 2017-06-09 中国海洋大学 The measuring method of one seed oyster parent's shellfish gonad maturity and application
CN106804492B (en) * 2017-01-19 2019-08-13 中国海洋大学 The measurement method and application of one seed oyster parent's shellfish gonad maturity
CN114642191A (en) * 2021-12-08 2022-06-21 连云港市农业科学院 Method for hybridization between different bean hawkmoth geographic populations

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