CN1636140A

CN1636140A - Optical disc analysis system including related methods for biological and medical imaging

Info

Publication number: CN1636140A
Application number: CN02817842.4A
Authority: CN
Inventors: 马克·沃斯恩登
Original assignee: Burstein Technologies Inc
Current assignee: Burstein Technologies Inc
Priority date: 2001-07-12
Filing date: 2002-07-12
Publication date: 2005-07-06
Also published as: EP1588355A2; WO2003007293A2; WO2003007293A8; WO2003007293A3

Abstract

An optical disc system includes a photo detector circuit of an optical disc drive and a signal processing system. The photo detector circuit is configured to generate at least one information-carrying signal from an optical disc assembly. The signal processing system is coupled to the photo detector circuit to obtain from the at least one information-carrying signal both operational information used to operate the optical disc system and data indicative of presence and/or characteristics of an investigational feature associated with the optical disc assembly. Methods and discs for imaging a biological or medical investigational feature are also provided.

Description

The optical disc analysis system that comprises biological and medical imaging method

Technical field

The present invention relates generally to CD and CD reader.The present invention is specifically related to utilize the standard optical disc driver and the driver of retrofiting slightly, allows can gather various dissimilar signals from CD with differentiating.The CD of this purposes comprises: the photo bio dish of coded message and investigation structure or feature is arranged, wherein investigate outside surface or inside surface that structure or feature are deposited on dish.

Background technology

The common unsettled U.S. Patent application No.09/183 that transfers the possession of, 842 and 09/311,329 describes the method and apparatus of operation and investigation structure on the one or more surfaces that are used to detect the CD assembly.By some treatment circuit in the reality change CD drive, certain methods of discussing in these applications and Equipment Inspection investigation structure.Another kind as these and similar approach is selected, and the present invention utilizes the major advantage of standard optical disc reader to carry out laser capture microdissection to detect.This advantage comprises the common distribution of this driver in current consumer's environment.So, it is desirable to, need not actually changing under the condition of treatment circuit, be provided for detecting the method and apparatus of operating and investigate structure on the CD assembly.

To thoroughly discuss backgrounds more of the present invention in order providing, to describe the correlated characteristic of conventional CD reader and CD in conjunction with Fig. 1-7 briefly.In order to do preliminary introduction, these accompanying drawings are described at first briefly.Fig. 1 is typical individual layer CD or the sectional view of class CD dish and the synoptic diagram of associated reader.Fig. 2 is the amplification lateral sectional view of CD shown in Figure 1.Fig. 3 is the CD-R panel surface skeleton view that swinging chute is arranged.Fig. 4 is the synoptic diagram of CD detecting device and interlock circuit, and this circuit utilizes three light beams to follow the tracks of, and focuses on and reads.Fig. 5 be in the typical three light beam pick-ups light-beam position with respect to the plan view of magnetic track on the CD.Fig. 6 is the block scheme of known optical disc reader.Fig. 7 is the functional-block diagram of conventional digital signal processing circuit.

More particularly, Fig. 1 describes light picker or the objective lens unit 10 and the conventional CD type CD 11 of reader, and wherein dotted line is represented light path.For simple and clear, Fig. 1 a spot of smooth pick-up part that draws.Fig. 2 provides the amplification lateral sectional view with the CD 11 of incident light same orientation.

With reference to Fig. 1 and 2, conventional light picker 10 comprises: light source 19,

lens

12,13 and 14, beam splitter 15, quarter wave plate 20 and detecting device 18.Light source 19 is positioned at the focus of the collimation lens 12 that long-focus is arranged usually.Collimation lens 12 makes divergent rays become directional light.Monitor diode (not shown) can be used for the output of stable laser.Light source 19 can be a laser instrument, and LED or laser diode are though the present invention also can implement in incoherent light system.

The conventional optical design of three light beam pick-ups utilizes two secondary beam to follow the tracks of usually.In order to produce these light beams, by diffraction grating 17, diffraction grating 17 is apertured panels from the light transmission of light source 19, the slit be spaced apart several optical maser wavelengths.When beam Propagation is passed through this grating, light generation diffraction; When the light beam that forms was focused on once more, it formed single bright central light beam, the light beam that has a series of intensity to weaken in succession around it.Actual light beam this diffraction pattern just that incides on the CD.

Three conventional light beam pick-ups utilize central light beam sense data and focusing, and two secondary beam only are used for following the tracks of.In this design, light beam is a space relationship, because they are from single diffracted laser beam.Contrast therewith, the single beam pick-up utilizes single light beam to finish all these tasks.

Polarising beam splitter 15 (PBS) direct light is to optical disc surface, and then direct reflected light is to photodiode sensor 18.PBS 15 comprises two prisms usually, and its 45 ° of common faces are as polarizing prism.Collimation lens 12 is preferably after PBS 15.Light transmission is by quarter wave plate 20, and quarter wave plate is the anisotropic material of rotary beam plane of polarization.Transmission is reflected the only linearly polarized light that returns again by quarter wave plate 20 by quarter wave plate 20 and from CD 11, and its plane of polarization and incident light polarization face meet at right angles.Because PBS 15 transmission is along the light (for example, the horizontal polarization face) of a plane of polarization, and reflection is along the light (for example, vertical polarization face) of another plane of polarization, and therefore, PBS 15 deflects into sensor 18 to folded light beam with the reading number data.

Last optical element in dish 11 light paths that deluster is object lens 13, and it is used for focused beam to the dish data surface, wherein considers the refractive index of the polycarbonate substrate 112 of dipped beam.Object lens 13 are focused into this light light cone or the hot spot that converges.Degree of focus is the function of lens numerical aperture.

Now, coded data is determined the result of laser on the CD 11.In normal CD, when hot spot hit the platform at smooth interval between two holes, light was almost by total reflection.When hot spot hits the degree of depth and is about the hole of 1/4 optical wavelength, with disappearing mutually a spot of light is reflected because of interfering the diffraction that produces.The light beam of all three intensity modulated returns by object lens 13, quarter wave plate 20, collimation lens 12 and PBS 15.At last, these beam Propagation are by simple lens 14 and astigmatism element 16, and astigmatism element 16 can be a cylindrical lens, and it introduces astigmatism in the folded light beam of electric diode 18 light paths of delustering.

As detail display among Fig. 2, CD type dish 11 comprises three layers from the dipped beam face to the distance light face.By convention, the numbering of dish layer is upward to the distance light face from the dipped beam face.These layers comprise: transparent substrate 112, reflection horizon 114 and protective seam 116.Measure along optical axis direction, transparent substrate 112 constitutes most of thickness of typical CD type dish, and provides the CD operation required optics and architectural feature.

Transparent substrate 112 normally utilizes the spiral path impression to make, and spiral path is the most inboard readable part from dish, outwards rotates to the readable part of outermost of dish then.In the dish (for example, pre-recorded disc) of non-record, this magnetic track is that the hole by a series of impressions constitutes, and the degree of depth in each hole is about 1/4 optical wavelength of reading this dish usually.Each cheats vicissitudinous length.Utilize the length and the mechanism of interval in hole as coded data.

Referring now to Fig. 3, the spiral slot in the CD-R comprises dyestuff rather than hole.Typical CD-R comprises the spiral slot that character shape is arranged along its length direction.Such groove is referred to as " swinging chute ", and the waveform sidewall is arranged at its bottom.Raised portion is separated groove adjacent in the spirality.This swinging chute can comprise projection shown in Figure 3 110 and groove part 118.The standard that is similar to projection 110 and groove part 118 can write down the swinging chute among the CD.

Referring now to Fig. 4, Fig. 4 describes typical detecting device 18 and relevant circuit thereof in more detail.Detecting device 18 generally includes center detector 25, and additional

side detector cell

26 and 27 is arranged around it.Center detector 25 can be divided into a plurality of detector cells (for example, 4), and they are expressed as A, B, C and D.Detector cell A, B, each detector cell provides an electric signal among C and the D (being referred to as " quaternary detecting device " sometimes), and it points out to incide the intensity of folded light beam on this unit.

From the signal sum of quaternary detecting device 25, for example, A+B+C+D provides radio frequency (RF) signal 50, also is referred to as high frequency (HF) signal, quaternary summation or summing signal.Mark used herein " A+B " expression is from the signal sum of detector cell A and B.HF signal 50 (that is, RF signal, quaternary summation or summing signal) is usually by the data of demodulation to recover to write down on the CD.

Also signal being made up, be provided for following the tracks of feedback signal with focus control among the detector cell A to F each.For example, (that is, E-F), can obtain tracking signal 52 (for example, tracking error or TE signal) according to the poor of E signal and F signal.Poor according to A+C signal and B+D signal can obtain focusing error (FE) signal 54.

Usually, this processing operation is finished by mimic channel and in conjunction with one or more integrated circuit (IC) chip.Mimic channel often adopts the form of special chip group or single ASIC (special IC) chip.

Circuit among Fig. 4 only is an example of this circuit, and it provides focus error signal and tracking error signal in CD player.Known have several different methods that these signals can be provided.For example, U.S. Patent No. 5,629, the critical angle method of describing in 514, or The CompactDisc Handbook, Pohlmann, A-R Editions, Inc. Foucault and astigmatism method of describing in (1992) utilizes these methods can obtain focus error signal, they is incorporated in full this is for reference.Similarly, i-beam push-pull or three beam method described among the The Compact Disc Handbook, or U.S. Patent No. 5,130, the differential phase method of describing in 963, this literary composition is incorporated in that this is for reference, or single beam swing in high frequency method, utilizes these methods can obtain tracking error signal.

Referring now to Fig. 5, the CD driver utilizes three light beam pick-ups usually, and wherein light beam is divided into three light beams, that is, and and a main beam 21 and two tracking light beams 23.Main beam 21 focuses on the surface of CD, so it is positioned at the center of tracking structure, and follows the tracks of the relative both sides that light beam 23 incides tracking structure.Main beam 21 be at the center of magnetic track 24 (as cheat 22 determine), follow the tracks of the relative both sides that light beams 23 incide magnetic track 24 for two.By design, three light beams are from CD reflection, and are directed into detecting device 18 (Fig. 4), and therefore, main beam 21 incides the quaternary detecting device, incide detector cell E and F and follow the tracks of light beam 23.

Fig. 6 is the schematic block diagram of typical light drive system chips group 30.Though CD has nothing in common with each other between the chipset of CD-R and DVD driver is mutual, should be understood that system shown in Figure 6 roughly represents all types of CD-ROM driver, the difference between each chipset of detail knowledge is optional for putting into practice the present invention.

To from detector cell A, B, the signal summation of C and D and the HF signal 50 that obtains is handled is so that extract all data that write down on the CD.At first, utilize the operation of normalization and equalization, simulation HF signal 50 is handled.Secondly, simulating signal 50 is converted to digitized signal, comprise the serial digital data stream that is referred to as channels bits.Then, according to reading the used modulation standard of the type CD, demodulated channel bit stream.For example, CD type dish utilizes 8-14 (be also referred to as 14 8) modulation (EFM) usually, and wherein data byte or 8 data bit are encoded into 14 channels bits.3 merge bits are arranged between every group of 14 channels bits.Therefore, when reading CD type CD, read 17 channels bits, abandon 3 merge bits from CD, remaining 14 decoded or demodulation, thus obtain original data byte.These data bytes are combined into piece, again piece are handled to reduce the effect of compact disc defect, for example, the cut on the optical disc surface.

Comparer 31 can convert the HF signal 50 (Fig. 4) that comes self-detector 18 to square-wave signal 51, and when HF signal 50 was higher than threshold value, it provided high output; And when HF signal 50 was lower than threshold value, it provided low output.Then, the square-wave signal 51 that obtains of digital signal processing circuit (DSP) 32 sampling is to determine the value of each channels bits.DSP 32 also demodulated channel position is combined into piece to these data bytes then and handles to proofread and correct contingent mistake to extract data byte.After data were handled by DSP 32 and are combined into piece, storer 33a provided the temporary transient storage of these data.

Servo block 34 is analyzed tracking error (TE) signal 52 (or seam tracking through weaving torch errors (WTE) in DVD or the CD-R system), and provides tracking control signal to keep correct tracking to follower to guarantee pick-up.Similarly, based on focusing error (FE) signal 54, provide focus control signal 53.DSP 32 provides the data rate indication of HF signal 50, and servo block 34 utilizes it that the spindle motor (not shown) of rate controlled signal 55 to CD drive is provided.

In audio CD player, after DSP 32 handles, send each data block unillustrated audio reproduction circuit in Fig. 6.Yet in some data-storage applications, each data block can comprise additional error-detecting code (EDC) and error-correcting code (ECC).By detecting and proofread and correct the mistake that DSP 32 does not have correction, EDC/ECC circuit 35 utilizes EDC and ECC sign indicating number to increase the integrality of data block usually.Storer 33b can make up with storer 33a, the data block that temporary transient storage is handled by EDC/ECC circuit 35.At last, by means of interface circuit 36, from the CD player transfer data blocks to main frame 37.Though what draw is the ATAPI interface, should be understood that the interface that also can utilize other, for example, and SCSI, Firewire, or USB (universal serial bus) (USB), or the like.

For example, by coordinating the transfer of data block between DSP 32 and the EDC/ECC circuit 35, controller 38 is coordinated the operation of each parts in the chipset 30.Controller 38 also monitors the data block that is being read out, and can monitor the various parameters of pointing out the CD reader running status.

Program storage 39 comprises the procedure code of controller 38 operations.In many CD reader chipsets, program storage 39 can comprise the programmed instruction of DSP 32 or EDC/ECC circuit 35.This is favourable for manufacturer, wherein by the procedure code in the reprogramming storer 39, can change the operation of disk drive.For example, by reprogramming storer 39, can be adapted to modulation or method of coding data on the CD newly developed.

Fig. 7 is the functional-block diagram that signal Processing takes place in the dsp chip 32 that disposes in a conventional manner.As shown in Figure 7, DSP 32 finishes a plurality of functions.For example, DSP 32 common normalization and/or balanced HF signal (square frame 40); Conversion is from the normalization HF signal (square frame 42) of analog to digital converter; The EFM signal (square frame 44) that the demodulation sign indicating number forms; Finish certain type error-checking process (for example, utilizing intersection to insert Reed-Solomon sign indicating number " CIRC ") (square frame 46); And the signal that formation is provided is to output interface (square frame 48), so that communicate with host data bus 37 (Fig. 6).The example of finishing the general dsp chip of some or all of these functions comprises: SAA 7210, SAA 7220 and SAA 7735, and they can be buied from the Philip electronics corporation of Dutch Eindhoven.

Though above description is enough to roughly understand content of the present invention,, the light picker that may use under linguistic context of the present invention and interlock circuit can have multiple different design and structure.More detailed content and design proposal are to describe in following file, and they are CompactDisc Technology, Nakajima and Ogawa, IOS Press, Inc. (1992); The Compact Disc Handbook, Digital Audio and Compact DiscTechnology, Baert et al. (eds.), Books Britain (1995); CD-RomProfessional ' s CD-Recordable Handbook:The Complete Guide toPractical Desktop CD, Starrett et al. (eds.), ISBN:0910965188 (1996); The full text of all these files is incorporated in that this is for reference.

Summary of the invention

An object of the present invention is to overcome limitation of the prior art.Another object of the present invention is to make existing optical disk system be suitable for reading the CD assembly also to extract the operation information that is used for the operational light disc system and point out the flag data that the investigation feature relevant with the CD assembly exists.

These and other objects and advantages of the present invention realize that in optical disk system this optical disk system comprises: the photo detector circuit of CD drive and signal processing system.The photo detector circuit of CD drive is configured to produce one at least from the CD assembly and carries information signal.Signal processing system is coupled to photo detector circuit, and at least one carries operation information and mark or the performance data of pointing out that the investigation feature relevant with the CD assembly exists that information signal obtains being used for the operational light disc system from this.

The present invention also provides a kind of method, and this method comprises: the deposition test sample book, the rotary CD assembly is inducted into irradiating light beam, detects Returning beam, and the Returning beam that processing detects is to obtain the investigation characteristic information relevant with test sample book.The step of deposition test sample book is placed on precalculated position on the CD assembly to sample.The step of rotary CD assembly is the CD assembly in the rotary CD driver.The step that is inducted into irradiating light beam directs the beam onto on the CD assembly.The step that detects Returning beam detects the Returning beam of incident beam and test sample book interaction formation.

Other advantages of the present invention obtain in another method, this method comprises: obtain a plurality of simulating signals, first subclass of a plurality of simulating signals of suing for peace, make up second subclass of a plurality of simulating signals, obtain being used for the information of operational light disk drive, and summing signal is converted to digitized signal.The step that obtains a plurality of simulating signals comprises: utilize a plurality of photoelectric detectors to receive back light from the CD assembly.The step of a plurality of simulating signal first subclass of suing for peace produces summing signal.The step that makes up a plurality of simulating signal second subclass produces tracking error signal.The step that obtains being used for operational light disk drive information comprises: handle tracking error signal.

Another method of the present invention comprises: transform the signal processing system of part, obtain a plurality of simulating signals, analog signal conversion is become digitized signal, and based on digitized signal, describe the investigation feature on the CD assembly.The step of transforming the part signal disposal system is to transform this system to move as analog to digital converter.The step that obtains a plurality of simulating signals is the photo detector circuit picked up signal from CD drive.A plurality of simulating signals comprise the information of indication investigation feature.It is to utilize the signal processing system switching signal that analog signal conversion is become the step of digitized signal.

The specific embodiments of this method comprises: receiving step and switch process.Receiving step comprises: the corresponding input end in signal processing circuit receives a simulating signal at least.This at least one simulating signal is provided by at least one corresponding photodetector unit, and this photodetector unit detects the light that returns from the CD assembly surface.Switch process comprises: each conversion of signals in this at least one simulating signal is become corresponding digitized signal.Each digitized signal is proportional to basically returns light intensity, and this back light is detected by a corresponding unit in this at least one photodetector unit.

More particularly, the invention provides a kind of optical disk system that comprises photo detector circuit in the CD drive, it is configured to produce one at least from the CD assembly and carries information signal.This optical disk system also is equipped with the signal processing system that is coupled to photo detector circuit, can at least one carry operation information and the flag data of pointing out that the investigation feature relevant with the CD assembly exists that information signal obtains being used for the operational light disc system from this.

In one embodiment, signal processing system comprises: PC and be coupling in this at least one carry analog to digital converter between information signal and the PC.Analog to digital converter can advantageously provide digitized signal, and PC can comprise first program module, is used for detecting and describing the peak value of digitized signal.In this embodiment, PC can also comprise second program module, is used for detecting the bimodal number with the compute digitized signal.In addition, signal processing system can also comprise: the analyzer that is coupled between analog to digital converter and the PC.In this embodiment, analog to digital converter provides digitized signal, and analyzer comprises the logic that is used for detecting and describing the digitized signal peak value.According to the aspect of this embodiment, analyzer also comprises the logic that is used for detecting and calculating the bimodal number of digitized signal.

Perhaps, optical disk system can comprise: signal processing system, this signal processing system have be coupling in this at least one carry audio processing modules between information signal and the analog to digital converter.In this another embodiment, optical disk system also comprises: predetermined sound that writes down on the CD assembly and the program module among the PC, when having the investigation feature, program module is used to detect this, and at least one carries the flag data of information signal and predetermined sound deviation.Predetermined sound can be that coding is quiet.

In another embodiment, signal processing system also comprises: this at least one carry the impact damper that is coupled between information signal and the analog to digital converter.Signal processing system can also advantageously comprise: be coupled to the detection trigger circuit of analog to digital converter, the detection trigger circuit can detect with this at least one and carry special time relevant when having flag data in the information signal.

In another embodiment of the invention, signal processing system comprises: programmable digital signal processor, be configured to selectively like this, at least one carries information signal and extracts operation information from this when first configuration, and moves so that flag data to be provided as analog to digital converter when second configuration.An aspect according to this embodiment of the present invention, signal processing system can comprise: PC, at least one carries the programmable digital signal processor of information signal to be coupled to this, and is coupling in the analyzer that flag data is provided between programmable digital signal processor and the PC.

In many specific embodiments of the present invention and embodiment, signal processing system can advantageously be equipped with the detection trigger circuit in cycle detection time, and in this time cycle, the investigation feature relevant with the CD assembly scanned by photo detector circuit; Or outfit detects the detection trigger circuit of the certain trigger time relevant with each duration, in this duration, flag data is present in this, and at least one carries in the information signal, and each duration is recurrent, has separately investigation feature and corresponding flag data group.

According to an aspect of audio frequency embodiment of the present invention, signal processing system comprises: PC and be coupling in PC and this at least one carry audio processing modules between the information signal.Audio processing modules can be selected from following a kind of: be independent of the external module of CD drive, and as the Drive Module of part CD drive, or as the remodeling Drive Module of part CD drive.PC can advantageously comprise: be coupled to saved software module in the processor of audio-frequency module and the storer, be used for processor controls and extract flag data from voice data.

In main embodiment of the present invention, photo detector circuit comprises the generation simulating signal, and at least one carries the circuit of information signal as this.Simulating signal comprises following a kind of: from the high-frequency signal of photoelectric detector, tracking error signal, focus error signal, the automatic gain control setting is recommended tracking signal, the CD tracking signal, the CDR tracking signal, focus signal, differential phase detection device signal, laser power monitor device signal, and voice signal.

According to other aspects of the present invention, optical disk system can comprise: the CD assembly is having the encoding operation information that relevant investigation feature is set and is used for the operational light disk drive in all the other dish sectors on the first dish sector.The CD assembly can comprise: reflection-type or transmission-type CD.The CD assembly can comprise the triggered mark that is provided with respect to the first dish precalculated position, sector.In this embodiment, signal processing system comprises: the detection trigger circuit that is used to detect triggered mark.

According to some feature of the present invention, detection trigger circuit period property ground detects triggered mark.Based on precalculated position triggered mark with respect to the first dish sector, (i) schedule time before photo detector circuit is read each investigation feature, when (ii) reading, or the schedule time after (iii) reading, the detection trigger circuit can detect triggered mark.

In other designs of a main embodiment of optical disk system, one or more additional photo detector circuits are configured to produce at least one and carry information signal from each CD assembly.The CD assembly can comprise one or more indicator, and this indicator has affinity to each investigation feature.One or more indicator can be selected from following one group respectively: plastic microsphere, colloid gold bead, silica bead, beaded glass, latex beads, polystyrene bead, magnetic bead, and fluorescent bead.

According to another aspect of the present invention, a kind of assay method is provided, this method may further comprise the steps: (1) precalculated position deposition test sample book on the CD assembly, (2) rotary CD assembly in CD drive, (3) be inducted into irradiating light beam to the CD assembly, (4) detect the Returning beam that incident beam and test sample book interact and form, handle the Returning beam of detection to obtain the investigation characteristic information relevant with test sample book with (5).In this method, the CD assembly can comprise one or more indicator, and this indicator has affinity to the investigation feature in the test sample book.These indicator can be selected from following one group respectively: plastic microsphere, colloid gold bead, silica bead, beaded glass, latex beads, polystyrene bead, magnetic bead, and fluorescent bead.

In this embodiment of this method, the step that detects Returning beam can form a plurality of simulating signals.Handling the Returning beam step that detects can advantageously comprise: first subclass of a plurality of simulating signals of (1) summation is to produce summing signal, (2) first subclass of a plurality of simulating signals of combination and a subclass in second subclass are to produce tracking error signal, (3) information and (4) that obtain being used for the operational light disk drive from tracking error signal convert summing signal to digitized signal.This method can randomly comprise additional step: detect the triggered mark relevant with the CD assembly.

Another kind of assay method according to some feature of the present invention may further comprise the steps: (1) precalculated position deposition test sample book on the CD assembly, (2) rotary CD assembly in CD drive, (3) be inducted into irradiating light beam to the CD assembly, (4) detect the interact transmitted light beam that forms and continuing of incident beam and test sample book, handle the transmitted light beam that detects with the acquisition investigation characteristic information relevant with test sample book with (5) by the CD assembly.This method can also comprise other steps: detect the folded light beam that incident beam and test sample book interact and form, and handle the folded light beam that detects with the acquisition investigation characteristic information relevant with test sample book.This method can also include the CD assembly of one or more indicator, and this indicator has affinity to the investigation feature in the test sample book.As method discussed above, in this method, one or more indicator can be selected from following one group respectively: plastic microsphere, colloid gold bead, silica bead, beaded glass, latex beads, polystyrene bead, magnetic bead, and fluorescent bead.

In addition, in this method, the step that detects transmitted light beam forms a plurality of simulating signals.Similarly, the step of handling transmitted light beam can comprise additional step: first subclass of a plurality of simulating signals of (1) summation is to produce summing signal, (2) first subclass of a plurality of simulating signals of combination and a subclass in second subclass are to produce tracking error signal, (3) information and (4) that obtain being used for the operational light disk drive from tracking error signal convert summing signal to digitized signal.This method can randomly comprise additional step: detect the triggered mark relevant with the CD assembly.

Another method of the present invention may further comprise the steps: (1) utilizes one or more photoelectric detectors, obtain a plurality of simulating signals from the CD assembly, (2) first subclass of a plurality of simulating signals of summation is to produce summing signal, (3) second subclass of a plurality of simulating signals of combination is to produce tracking error signal, (4) information and (5) that obtain being used for the operational light disk drive from tracking error signal convert summing signal to digitized signal.In this another method, obtain step and summation step and produce summing signal, summing signal comprises the disturbance of pointing out the investigation feature of locating on the CD module position.This method can also comprise step: describe the investigation feature based on digitized signal.

In this method, switch process can comprise: the CD drive chipset of configuration section moves as analog to digital converter.In one embodiment, this configuration step comprises: the program numbers signal processing chip moves as analog to digital converter in the CD drive chipset.Digital signal processing chip can advantageously be equipped with the normalization function, analog-digital conversion function, demodulate/decode function and output interface function.In this specific embodiment, configuration step can also comprise: by setting up a paths from analog-digital conversion function to the output interface function, the bypass summing signal is to avoid the demodulate/decode function.In addition, configuration step can also comprise: deactivation demodulate/decode function.

According to a main aspect of this method, switch process comprises: the allocating digital signal process chip, this chip comprises the normalization function, analog-digital conversion function, demodulate/decode function and output interface function.In addition, configuration step comprises: set up a paths from analog-digital conversion function to the output interface function, make summing signal not by the demodulate/decode function treatment.Herein, configuration step can comprise similarly: deactivation accent/decoding function.

According to an aspect of this method, obtaining step can comprise: one or more signals in a plurality of simulating signals of tap directly in one or more photoelectric detectors.In addition, switch process can comprise: guide these signals to enter analog to digital converter.In a specific embodiment, switch process also comprises: before analog to digital converter was handled, guiding entered buffer amplifier from the simulating signal of one or more photoelectric detectors.

Perhaps, obtaining step can comprise: after the CD drive chipset is handled, and the one or more signals in a plurality of simulating signals of tap, and switch process can comprise: guide these signals to enter analog to digital converter.In this another embodiment, switch process can comprise similarly: before the guiding simulating signal entered analog to digital converter, guiding entered buffer amplifier from the simulating signal of CD drive chipset.

According to another aspect of the present invention, another kind of method is provided, this method may further comprise the steps: (1) is transformed the signal processing system of part and is moved as analog to digital converter, (2) from the photo detector circuit of CD drive, obtain a plurality of simulating signals, a plurality of simulating signals comprise the information of pointing out investigation feature on the CD assembly, (3) utilize signal processing system, become analog signal conversion digitized signal and (4) to describe investigation feature based on digitized signal.In this embodiment, adaptation step can comprise: the digital signal processing chip in the programming signal disposal system moves as analog to digital converter.

May further comprise the steps according to another method of the present invention: (1) is at the corresponding input end of signal processing circuit, receive each signal at least one simulating signal, this at least one simulating signal is provided by at least one corresponding photodetector unit, this photodetector unit detects the light that returns from the CD assembly surface, (2) each conversion of signals in this at least one simulating signal is become corresponding digitized signal, each digitized signal is proportional to the back light that a corresponding unit detects in this at least one photodetector unit basically.In this method, switch process can advantageously comprise: the operation signal treatment circuit is with any demodulation of bypass first digitized signal.In this embodiment, switch process can also may further comprise the steps: (1) operation signal treatment circuit with any decoding of bypass first digitized signal and (2) operation signal treatment circuit with any bug check in bypass first digitized signal.

Perhaps, switch process can comprise: the operation signal treatment circuit is with any decoding of bypass first digitized signal.As another kind of method, switch process can comprise: the operation signal treatment circuit is with any bug check in bypass first digitized signal.

Each embodiment can also comprise step among the different embodiment of this method: when having two or more sort signal, make up at least two signals in this at least one simulating signal.In this embodiment, combination step is to be selected from one group of following operation: add, subtract, remove and take advantage of, and any combination.Combination step can be carried out before switch process, or carried out after switch process.

According to the method for claim 55, wherein receiving step comprises: at least one corresponding photodetector unit provides at least one simulating signal, and this photodetector unit detects the light of transmission by the CD assembly.

In general, in this method, receiving step can comprise: detect and point out the triggered mark of time cycle, in this time cycle, the investigation feature relevant with the CD assembly is by this at least one photoelectric detector scanning.In addition, these any embodiment can also comprise step: after switch process, provide first digitized signal of this at least one digitized signal at the output interface of signal processing circuit, it does not change switch process substantially and first digitized signal between the step is provided.In these embodiments, signal processing circuit comprises: digital signal processor or outside analog to digital converter.In the embodiment of A/D converter, signal processing circuit can also comprise: the buffer amplifier before the outside analog to digital converter.

According to other aspects of the present invention, the characteristic signal of Chan Shenging belongs to the present invention herein.Therefore, the invention still further relates to the characteristics of signals of investigation characteristic information on the relevant CD assembly, the process that produces this signal may further comprise the steps: (1) is in the precalculated position of CD assembly deposition test sample book, (2) rotary CD assembly in CD drive, (3) be inducted into irradiating light beam to the CD assembly, (4) detect the Returning beam that incident beam and test sample book interact and form, handle the Returning beam of detection to obtain the investigation characteristic information relevant with test sample book with (5).Returning beam can be formed by the interaction of incident beam and one or more indicator, and indicator has affinity to the investigation feature in the test sample book.The step that detects Returning beam can form a plurality of simulating signals.In this embodiment, the step of handling the Returning beam that detects can comprise: first subclass of a plurality of simulating signals of (1) summation is to produce summing signal, (2) first subclass of a plurality of simulating signals of combination and a subclass in second subclass are to produce tracking error signal, (3) information and (4) that obtain being used for the operational light disk drive from tracking error signal convert summing signal to digitized signal.This signal can comprise the different disturbances of pointing out investigation feature on the CD assembly.As mentioned above, switch process can comprise: the CD drive chipset of configuration section moves as analog to digital converter.In addition, configuration step can comprise: the digital signal processing chip in the programming CD drive chipset moves as analog to digital converter.

At a specific embodiment that is used for producing the desired signal process, digital signal processing chip comprises: normalization function, analog-digital conversion function, demodulate/decode function and output interface function.In this embodiment, configuration step can also comprise: by setting up a paths from analog-digital conversion function to the output interface function, the bypass summing signal is to avoid the demodulate/decode function.In addition, configuration step can also comprise: deactivation demodulate/decode function.

At another specific embodiment that is used for producing required signature signal process, switch process can comprise: the guiding summing signal enters outside analog to digital converter.In this embodiment, switch process can also comprise: the guiding summing signal enters the buffer amplifier before the outside analog to digital converter.

At another specific embodiment that is used for producing the desired signal signature process, switch process can comprise: the allocating digital signal process chip, it comprises the normalization function, analog-digital conversion function, the demodulate/decode function, with the output interface function, and configuration step comprises: set up a paths from analog-digital conversion function to the output interface function, make summing signal not by the demodulate/decode function treatment.

In these many signal production processes, detecting step can also comprise: detect the transmitted light beam that incident beam and test sample book interact and form, and transmit it by the CD assembly.

In addition, the step that detects Returning beam can form a plurality of simulating signals, and the step of handling the Returning beam that detects can comprise: first subclass of a plurality of simulating signals of (1) summation is to produce summing signal, (2) second subclass of a plurality of simulating signals of combination is to produce tracking error signal, (3) information and (4) that obtain being used for the operational light disk drive from the track error signal convert summing signal to digitized signal.In these embodiments, summing signal advantageously comprises the disturbance of pointing out investigation feature on the CD assembly.

Switch process can comprise: the CD drive chipset of configuration section moves as analog converter.In these embodiments, configuration section can comprise: the digital signal processing chip in the programming CD drive chipset moves as analog converter.In a more particular embodiment, digital signal processing chip comprises: normalization function, analog-digital conversion function, demodulate/decode function and output interface function.In these specific embodiments, configuration step can also comprise: by setting up a paths from the analog-converted function to the output interface function, the transmission summing signal is to avoid the demodulate/decode function.In addition, configuration step can also comprise: deactivation demodulate/decode function.

At some embodiment that is used for producing the desired signal signature process, switch process comprises: the allocating digital signal process chip, and this chip comprises: normalization function, analog-digital conversion function, demodulate/decode function and output interface function; And configuration step comprises: set up a paths from the analog-converted function to the output interface function, make summing signal not by the demodulate/decode function treatment.

Unique signal signature of the present invention also can be produced by such process, this process comprises step: (1) is transformed the signal processing system of part and is moved as analog converter, (2) from the photo detector circuit of CD drive, obtain a plurality of simulating signals, wherein a plurality of simulating signals comprise the information of pointing out investigation feature on the CD assembly, (3) utilize signal processing system, become analog signal conversion digitized signal and (4) to describe investigation feature based on digitized signal.In this process, adaptation step can comprise: the digital signal processing chip in the programming signal disposal system makes it move as analog converter.In addition, obtaining step can comprise: the simulating signal before the tap optical drive impact damper.In addition, obtain step and can comprise the triggered mark signal of pointing out the time cycle, in this time cycle, the investigation feature relevant with the CD assembly scanned by photo detector circuit.

According to other aspects of the present invention, a kind of method that detects signal in the optical disk system is provided, this method may further comprise the steps: (1) produces the incident beam of known wavelength, (2) guide this light beam to comprising on the CD of investigating feature and (3) receive the Returning beam that irradiating light beam and investigation characteristic interaction form.In this method, CD can comprise one or more the investigation feature being had the indicator of affinity, and this indicator can interact with incident beam.One or more indicator can be selected from following one group respectively: plastic microsphere, colloid gold bead, silica bead, beaded glass, latex beads, polystyrene bead, magnetic bead, and fluorescent bead.In addition, in this method, receiving step can also comprise: receive the transmitted light beam that irradiating light beam and investigation characteristic interaction form, and transmit it and pass through CD.In general, receiving step can advantageously relate to and utilizes one or more photoelectric detectors.Receiving step can form a plurality of simulating signals of being handled by signal processing system.In addition, signal processing system can comprise: the outside analog to digital converter that is with or without relevant buffer amplifier.In utilizing the embodiment of buffer amplifier, before inner disc driver buffer circuit is handled, can the tap simulating signal.In the present invention's some embodiment aspect these, signal processing system can comprise: programmable digital signal treatment circuit or audio frequency processing circuit.

According to other aspects of the present invention, a kind of method of imaging investigation feature is provided, this method may further comprise the steps: (1) is in the precalculated position of CD assembly deposition investigation feature, (2) the CD assembly in the rotary CD driver, (3) be inducted into irradiating light beam to the CD assembly, (4) detect the Returning beam that incident beam and investigation characteristic interaction form, (5) handle the Returning beam that detects with the information that obtains relevant investigation feature and (6) imaging investigation feature based on this information.In this method, the CD assembly can be equipped with one or more indicator that investigation feature in the test sample book had affinity.One or more indicator can be selected from following one group respectively: plastic microsphere, colloid gold bead, silica bead, beaded glass, latex beads, polystyrene bead, magnetic bead, and fluorescent bead.In addition, in this method, the step that detects Returning beam can form a plurality of simulating signals, and treatment step comprises: analog signal conversion is become digitized signal.In this specific embodiment, treatment step relates to signal processing system, and signal processing system can comprise the outside analog to digital converter that is with or without buffer amplifier.Perhaps, signal processing system can be equipped with programmable digital signal treatment circuit and/or relevant audio frequency processing circuit.

In many embodiments of this concrete grammar, the step of handling the Returning beam that detects can also may further comprise the steps: first subclass of a plurality of simulating signals of (1) summation is to produce summing signal, (2) first subclass of a plurality of simulating signals of combination and a subclass in second subclass are to produce tracking error signal, (3) from tracking error signal, obtain being used for the information of operational light disk drive, (4) summing signal is converted to digitized signal and (5) export this digitized signal.In these embodiments, the output step can relate on monitor the display digit signal or utilize micropkonic sound playing digitized signal.

According to other aspects of the present invention, provide a kind of external member that is used for detecting test sample book investigation feature.This external member comprises the branch rack that can place one or more CDs.This external member also comprises one or more containers, and this container has one or more following one group reagent that are selected from: the nucleic acid of isolation, antibody, protein, reaction reagent, and indicator.This external member can also be equipped with at least one according to photo bio dish of the present invention and/or optical disc apparatus.

This external member can also comprise buffer amplifier card, and buffer amplifier card is suitable for being encased in CD drive.This external member can also comprise the remodeling CD drive.

According to other aspects of the present invention, provide a kind of optical analysis disc that is used for the detection signal element.This optical analysis disc comprises: substrate layer, the operation layer relevant with substrate layer, operation layer have the operation information of coding and the signal element of placing with respect to operation layer; Signal element and operation layer have optics or the magnetism characteristic of choosing, and can form predetermined contrast between them, thereby different return signal between the characteristic of pointing out the relevant information of operation layer and signal element is provided.Optics or magnetism characteristic include, but not limited to the electric or magnetic polarization state or the irradiance of signal element and/or operation layer.

According to other aspects of the present invention, provide a kind of optical analysis disc that is used for imaging biology or medical science investigation feature.This optical analysis disc comprises: substrate, the operation layer relevant with substrate, operation layer has the encoding operation feature with respect to the particular track spacing, with investigation feature with respect to operation layer, the size of the investigation feature of choosing is greater than the operating characteristics of correspondence, and is track pitch half at least, therefore, when incident beam when operating characteristics is followed the tracks of, the single pass of investigation feature is provided at least.In this embodiment, rotating speed that can console panel, thus in the return signal digitizing that dish produces, provide higher quantization resolution.This dish can also advantageously comprise and be linked into the logic that dish is gone up pre-addressed location at random.

Description of drawings

According to following description of the preferred embodiment of the present invention, other purposes of the present invention and feature and advantage are conspicuous, and in the accompanying drawing of the preferred embodiment of the present invention, identical reference number is represented identical parts, wherein:

Fig. 1 is the sectional view and the relevant therewith reader synoptic diagram of typical individual layer CD or class CD dish;

Fig. 2 is that the Zoom Side of dish shown in Figure 1 is to cross sectional side view;

Fig. 3 is the skeleton view that the CD-R panel surface of swinging chute is arranged;

Fig. 4 is the synoptic diagram of CD detecting device and interlock circuit, and it utilizes three light beams to follow the tracks of, and focuses on and reads;

Fig. 5 is the planimetric map with respect to light-beam position in typical case's three light beam pick-ups of magnetic track on the CD;

Fig. 6 is the block scheme of known optical disc reader;

Fig. 7 is the functional-block diagram of conventional digital signal processing circuit;

Fig. 8 is the chipset block scheme according to the remodeling general optic disc reader of one aspect of the invention monitor signal, is used for determining to investigate on the optical analysis disc existence of feature or investigation structure;

Fig. 9 A is the material object and the block scheme of other embodiments of the invention, is used to handle the high frequency of CD drive, follows the tracks of, and focuses on audio frequency or other signals and demonstration or export relative result;

Fig. 9 B is an amplification detailed perspective view of pointing out part among Fig. 9 A, and it shows the coordinate reference system of 3 dimension orientations;

Figure 10 is the known procedure process flow diagram for preparing CD and read CD subsequently;

Figure 11 is the improvement path that is used for decoding CD according to the present invention;

Figure 12 is the view that is similar to Fig. 9 A, and its shows CD assembly and investigation feature and in conjunction with according to optics and Returning beam in the CD reader of first embodiment of the invention and the driver;

Figure 13 is a disc plane view of showing target area and hardware trigger;

Figure 14 is the overall drive system block scheme according to one embodiment of the invention;

Figure 15 is the circuit board top view that another aspect of the present invention comprises the detection trigger assembly;

Figure 16 is the circuit diagram of trigger circuit shown in Figure 15;

Figure 17 shows according to the CD of some feature enforcement of the present invention and the in kind and part block scheme of part of reading system;

Figure 18 comprises used trigger among each embodiment of the present invention, the feature board block scheme of amplifier and detector circuit;

Figure 19 is the top plan view that removes the CD drive assembly of outer cover, and it shows main shaft, carriage assembly, head assemblies, and and head assemblies between transmit the ribbon-style cable or the connector of signal;

Figure 20 is the bottom perspective view of CD drive assembly shown in Figure 19, and it illustrates chipset, interlock circuit, and the practical layout of banded connector in the head assemblies of throwing off with circuit;

Figure 21 is the block scheme that is connected to the known optical disc reader shown in Figure 6 of impact damper card according to different embodiments of the invention;

Figure 22 is suitable for from the top perspective of the external buffer amplifier card of the head assemblies received signal that drives impact damper according to first embodiment of the invention;

Figure 23 is the skeleton view of the external buffer amplifier card shown in Figure 22 of another embodiment;

Figure 24 is Figure 24 A, the diagrammatic representation that concerns between 24B and the 24C;

Figure 24 A.24B and 24C are according to the circuit diagram of amplifier stage in first embodiment impact damper card shown in Figure 22 and 23;

Figure 25 is the functional-block diagram that becomes the digital signal processing circuit of analog to digital converter according to another embodiment of the present invention principle programmable configuration shown in Figure 9;

Figure 26 is shown in Figure 25 according to relating to the flow chart of steps that detects the investigation element in the second embodiment of the invention;

Figure 27 is the biological decomposition diagram that coils of reflection-type that uses in conjunction with the present invention;

Figure 28 is the top plan view of dish shown in Figure 27;

Figure 29 is the skeleton view of dish shown in Figure 27, and it cuts out the different layers that part is showed CD;

Figure 30 is the biological decomposition diagram that coils of transmission-type that uses in conjunction with the present invention;

Figure 31 is the skeleton view of CD shown in Figure 30, and it cuts out the semi-reflective layer functional character of part show-plate;

Figure 32 is the curve map that concerns between the thickness of gold thin film and the transmissivity;

Figure 33 is the top plan view of CD shown in Figure 30;

Figure 34 is the skeleton view of CD shown in Figure 30, and it cuts out part and shows each layer that comprises semi-reflective layer dish-type among Figure 31;

Figure 35 gets perpendicular to Figure 27, the cut-away section view of the biological dish of reflection type optical shown in 28 and 29 radius, and it shows the flow passage that wherein forms;

Figure 36 gets perpendicular to Figure 30, the cut-away section view of the dish of transmission-type photo bio shown in 33 and 34 radius, and it shows flow passage and the top detector that wherein forms;

Figure 37 is Figure 27, the part longitudinal sectional drawing of the biological dish of reflection type optical shown in 28 and 29, and it shows the swinging chute that wherein forms;

Figure 38 is Figure 30, the part longitudinal sectional drawing of the dish of transmission-type photo bio shown in 33 and 34, and it shows swinging chute and the top detector that wherein forms;

Figure 39 is the sectional view that is similar to Figure 35, and it shows the whole thickness of reflection-type dish and initial refractive properties thereof;

Figure 40 is the sectional view that is similar to Figure 36, and it shows the whole thickness of transmission-type dish and initial refractive properties thereof;

Figure 41 is the cross sectional side view according to CD assembly of the present invention, and it comprises anaclasis top cover and investigation feature;

Figure 42 is the typical case's three beam system planimetric maps that project on three magnetic tracks of CD;

Figure 43 is according to three the beam plane figures of the present invention with respect to three magnetic tracks, and the investigation feature is arranged on one of them magnetic track;

Figure 44 is the signature tune line chart corresponding to operating characteristics, comprises that utilization of the present invention can discern the hole or the platform of variation;

Figure 45 and Figure 46 describe the signal change curve that operating characteristics produces on the CD;

Figure 47 is a biology dish sectional view of showing the microstream passage according to the present invention;

Figure 48 is the material reflectance utilized according to the present invention typical curve with variation in thickness;

Figure 49 is a pair of fluctuating enveloping curve according to the amplification analog readout signal that reacts in the microstream passage of the present invention;

Figure 50 is according to biological dish of the present invention and the corresponding planimetric map of reading the test sample book signal;

Figure 51 is the photo bio dish cross sectional side view that comprises the pearl indicator that utilizes in conjunction with the present invention;

Figure 52 A is placed on diagrammatic representation on the photo bio disk track according to the blue pearl of two 6.8 μ m of the present invention;

Figure 52 B is used to a series of signature tracks from the derivation of pearl shown in Figure 52 A from the AC of CD-ROM driver coupling and buffered HF signal according to the present invention;

Figure 53 A is placed on diagrammatic representation on the photo bio disk track according to two 6.42 red pearls of μ m of the present invention;

Figure 53 B is used to a series of signature tracks from the derivation of pearl shown in Figure 53 A from the AC of CD-ROM driver coupling and buffered HF signal according to the present invention;

Figure 54 A is placed on diagrammatic representation on the photo bio disk track according to two 6.33 μ m of the present invention polystyrene bead;

Figure 54 B is used to a series of signature tracks from the derivation of pearl shown in Figure 54 A from the AC of CD-ROM driver coupling and buffered HF signal according to the present invention;

Figure 55 A is placed on diagrammatic representation on the photo bio disk track according to one 5.5 μ m of the present invention beaded glass;

Figure 55 B is used to a series of signature tracks from the derivation of pearl shown in Figure 55 A from the AC of CD-ROM driver coupling and buffered HF signal according to the present invention;

Figure 56 A is placed on diagrammatic representation on the photo bio disk track according to one 4.5 μ m of the present invention magnetic bead;

Figure 56 B is used to a series of signature tracks from the derivation of pearl shown in Figure 56 A from the AC of CD-ROM driver coupling and buffered HF signal according to the present invention;

Figure 57 A is placed on diagrammatic representation on the photo bio disk track according to the blue pearl of two 4.0 μ m of the present invention;

Figure 57 B is used to a series of signature tracks from the derivation of pearl shown in Figure 57 A from the AC of CD-ROM driver coupling and buffered HF signal according to the present invention;

Figure 58 A is placed on diagrammatic representation on the photo bio disk track according to one 2.986 μ m of the present invention polystyrene bead;

Figure 58 B is used to a series of signature tracks from the derivation of pearl shown in Figure 58 A from the AC of CD-ROM driver coupling and buffered HF signal according to the present invention;

Figure 59 A is placed on diagrammatic representation on the biological disk track according to two 2.9 white pearls of μ m of the present invention;

Figure 59 B is used to a series of signature tracks from the derivation of pearl shown in Figure 59 A from the AC of CD-ROM driver coupling and buffered HF signal according to the present invention;

Figure 60 A is placed on diagrammatic representation on the photo bio disk track according to four 2.8 μ m of the present invention magnetic bead;

Figure 60 B is used to a series of signature tracks from the derivation of pearl shown in Figure 60 A from the AC of CD-ROM driver coupling and buffered HF signal according to the present invention;

Figure 61 A is according to the present invention includes 2.8 μ m magnetic beads, and the pearl of blue polystyrene bead of 4.0 μ m and 6.8 μ m and different size silica bead mixes the diagrammatic representation that is placed on the photo bio disk track;

Figure 61 B is used to a series of signature tracks from bunch derivation of pearl shown in Figure 61 A from the AC of CD-ROM driver coupling and buffered HF signal according to the present invention;

Figure 62 A is placed on diagrammatic representation on the photo bio disk track according to the white fluorescence polystyrene bead of two 2.9 μ m of the present invention;

Figure 62 B is used to a series of signature tracks from the derivation of pearl shown in Figure 62 A from the DC of CD-ROM driver coupling and buffered HF signal according to the present invention;

Figure 63 A is placed on diagrammatic representation on the photo bio disk track according to the white fluorescence polystyrene bead of two 2.9 μ m of the present invention;

Figure 63 B is used to a series of signature tracks of deriving from pearl shown in Figure 63 A from the DC of CD-ROM driver coupling and buffering " A " signal according to the present invention;

Figure 64 A and Figure 64 B are the cross sectional side views that is similar to Figure 51, and it shows that in more detail the biochemistry between biological dish and the indicator pearl interacts;

Figure 65 is the cross sectional side view according to photo bio dish of the present invention, and it comprises the investigation feature of the contiguous red blood cell of placing as reading optical beam inquiry in the CD drive assembly;

Figure 66 A is about 6.0 μ m red blood cells according to the contiguous diameter of placing of the present invention to be placed on diagrammatic representation on the photo bio disk track;

Figure 66 B is used to a series of signature tracks from the derivation of red blood cell shown in Figure 66 A from the AC of CD-ROM driver coupling and buffered HF signal according to the present invention;

Figure 67 A is about 6.0 μ m red blood cells according to the contiguous diameter of placing of the present invention to be placed on diagrammatic representation on the photo bio disk track;

Figure 67 B is used to a series of signature tracks from the derivation of red blood cell shown in Figure 67 A from the DC of CD-ROM driver coupling and buffered HF signal according to the present invention;

Figure 68 is the cross sectional side view according to photo bio dish of the present invention, and it comprises away from the investigation feature of the red blood cell of placing as reading optical beam inquiry in the CD drive assembly;

Figure 69 A is about 6.0 μ m red blood cells according to two of the present invention away from the diameters of placing to be placed on diagrammatic representation on the photo bio disk track;

Figure 69 B is used to a series of signature tracks from the derivation of red blood cell shown in Figure 69 A from the AC of CD-ROM driver coupling and buffered HF signal according to the present invention;

Figure 70 A is about 6.0 μ m red blood cells according to two of the present invention away from the diameters of placing to be placed on diagrammatic representation on the photo bio disk track;

Figure 70 B is used to a series of signature tracks from the derivation of red blood cell shown in Figure 70 A from the DC of CD-ROM driver coupling and buffered HF signal according to the present invention;

Figure 71 is the top perspective according to optical test dish of the present invention, and its header field that cuts explanation is placed on the insect wing of checking in the passage;

Figure 71 A is the amplification vertical view of part shown in Figure 71, shows insect wing in more detail according to this embodiment of the present invention, checks passage, and the information stores magnetic track of CD and light beam incide the focal beam spot on the optical test disk track;

Figure 72 is the cross sectional side view of getting perpendicular to optical test dish radius among Figure 71, and it comprises that insect wing is as the investigation feature according to reading optical beam inquiry in the CD drive assembly of the present invention;

Figure 73 A is the diagrammatic representation of insect wing transversal section in Figure 71 A and 72, and it is placed in the inspection passage according to optical test disk track of the present invention;

Figure 73 B is used to the single signature track from the derivation of insect wing transversal section shown in Figure 73 A from the AC of CD-ROM driver coupling and buffered HF signal according to the present invention;

Figure 74 A is similar to the diagrammatic representation shown in Figure 73 A;

Figure 74 B is used to a series of four the signature tracks in succession from the derivation of insect wing transversal section shown in Figure 74 A from the AC of CD-ROM driver coupling and buffered HF signal according to the present invention;

Figure 75 A is similar to the diagrammatic representation shown in Figure 73 A;

Figure 75 B is used to the track of signature in succession from a series of intermediate densities of the derivation of insect wing transversal section shown in Figure 75 A from the AC of CD-ROM driver coupling and buffered HF signal according to the present invention;

Figure 76 A is similar to the diagrammatic representation shown in Figure 73 A;

Figure 76 B is used to the track of signature in succession from a series of higher densities of the derivation of insect wing transversal section shown in Figure 76 A from the AC of CD-ROM driver coupling and buffered HF signal according to the present invention;

Figure 77 A, 77B and 77C are used to respectively from the AC of CD-ROM driver assembly coupling and buffered HF signal, DC coupling and buffering " A " signal, or DC coupling and the insect wing diagrammatic representation from Figure 71 A and 72 that provides according to the inventive method of buffered HF signal;

Figure 78 is the diagrammatic representation that concerns between Figure 78 A and the 78B;

Figure 78 A and 78B are the circuit diagrams of second embodiment amplifier stage, and it can be implemented in the impact damper card shown in Figure 22 and 23 according to the present invention;

Figure 79 A, 79B, 79C and 79D are the cross sectional side views of photo bio dish, it shows the method that detects investigation feature in the test sample book;

Figure 80 A, 80B, 80C and 80D are the cross sectional side views of used photo bio dish during mixed-phase is measured, and it detects the investigation feature in the test sample book;

Figure 81 A, 81B, 81C, 81D, 81E and 81F are the cross sectional side views of photo bio dish, it shows the method for utilizing investigation feature in the ELISA detection test sample book;

Figure 82 is the detailed cut-away section view of biological panel surface, and it shows that the indicator pearl has specific affinity for the antigen that adheres on the surface;

Figure 83 A, 83B, 83C and 83D are the cross sectional side views of photo bio dish, it shows the method for indicator pearl with investigation feature in the detection test sample book of utilizing;

Figure 84 is the detailed cut-away section view of biological panel surface, and its displaying utilizes the indicator pearl, catches probe and signal probe to detect the investigation feature in the test sample book;

Figure 85 is the view that is similar to Figure 84, and it shows that the investigation feature is to catching probe and signal probe's hybridization;

Figure 86 is the cross sectional side view of biological dish, and it shows that the trapping region that utilizes coating antibody is to detect the analyte in the test sample book;

Figure 87 is that the technology synoptic diagram with the detection of biological position is read in interpolation good channels so position from CD;

Figure 88 A is the side view of hybrid optical disc device for carrying out said;

Figure 88 B is the top view of hybrid optical disc device for carrying out said;

Figure 89 A represents the prior art embodiment of objective lens unit in the user CD-R player; With

Figure 89 B represents to have in the CD-R player the additional objective lens unit embodiment of adjusting lens.

Embodiment

Need not actual change treatment circuit and utilize conventional disk drive, the invention provides the method and apparatus that is used to detect operation and investigation structure or feature on the CD assembly.According to one embodiment of the present of invention, one or more signal processing circuits are configured to move as modulus (A/D) converter able to programmely in the conventional disk drive.A/D converter is used to detect the investigation feature that is provided with on the CD assembly surface and the electrical patterns of structurally associated.Electrical patterns can be used for determining relative size, composition and the position of detected structure.Many unlike signals that can be used to self-driven device reproduce required electrical patterns.Existing different electronic signals can cause the different electrical patterns of identical investigation feature, disturbance, or " signature " in the driver.Yet, should be understood that each this signature or signal disturbance are unique, therefore, it can be used as the independent and difference designator of each investigation feature of being considered.Then, Chang Gui disk drive turns back to its primitive operation configuration able to programmely.We discuss internal drive and outside processing and the imaging software features relevant as this embodiment of the present invention.

According to an alternative embodiment of the invention, and, utilize the outside A/D converter that is with or without the external buffer card as another scheme of the one or more signal processing circuits of programmable configuration in disk drive as the operation of modulus (A/D) converter.In these embodiment of the present invention, a plurality of unlike signals that can also be used to self-driven device reproduce required electrical patterns.As above embodiment, existing different electronic signals can cause the different electrical patterns of identical investigation feature, signal disturbance, or " signature " in the driver.Be to be further appreciated that with respect to this embodiment of the present invention, each this signature is unique, therefore, it can be used as consider each investigation feature or its attribute separately and the difference designator.Processing and imaging software also are the features relevant of this embodiment of the present invention.

Electrical characteristics in the time of can utilizing light to enter objective lens unit in the CD drive are set up the multidimensional image of investigation feature on CD.The photo bio dish can be designed to be convenient to the collection of data and the foundation of image.

Can utilize CD drive as the optical disk image forming device, the signal element from the CD assembly (that is, by the sample of imaging) produces multidimensional image.Be collected into photoelectric detector the objective lens unit from the energy of CD or CD assembly surface reflection, and utilize its to reproduce multidimensional image.Can optimize properties of optical disks (machinery, logical OR optical property) and optical devices character to produce high-resolution multidimensional image, it describes signal element in some aspects discriminatively.

Can adjust the objective lens unit and the photoelectric detector of CD drive, be convenient to collect the high-definition picture of investigation feature on the optical disc surface.The quaternary detecting device of object lens inside (or in light path of object lens) often is combined into form shown in 18 among Fig. 4.

Referring now to Fig. 8,, can in its original configuration, replenish the chipset 30 of Fig. 4 by adding tap impact damper 57,58 and 59.These tap impact dampers provide access for respectively untreated simulating signal, for example, the HF signal 50 that detecting device 18 produces, TE signal 52 and FE signal 54, thus allow external unit under the condition of not disturbing the operation of driven device, to receive these signals.

Another kind of improvement project is to add the tap impact damper, thereby allows the signal A to F that is untreated of self-detector 18 to be handled by external unit or additional circuit.According to these signals, can form HF, TE, FE, or any other combination.In addition, any additional detecting device can provide the signal (for example, G and the H detecting device in the current state-of-the-art technology driver) of usefulness according to identical mode.Do not need the tap impact damper, some drive circuit design and detecting device/amplifier installation allow equipment or adjunct circuit to be directly connected to detecting device.

Secondly with reference to Fig. 9, Fig. 9 represents according to CD drive 140 of the present invention.CD drive 140 has pan arrest 168, and pan arrest 168 is suitable for holding tray 130, and this type disc is designed to hold various investigation features.Dish 130 can be the photo bio dish, for example, the common U.S. Provisional Application No.60/252 that transfers the possession of, 726, its title is " Bioactive Solid Phase for SpecificCell Capture and Optical Bio-Disc Including Same "; No.60/249,391, its title is " Optical Disc Assembly for Performing Microscope andSpectroscopy Using Optical Disc Drive "; And No.60/257,705, its title is " Surface Assembly for Immobilizing DNA Capture Probes andBead-Based Assay Including Optical Bio-Discs and Methods RelatingThereto " disclosed photo bio dish.

Known CD drive often is encapsulated in the casing that comprises CD drive 140 and one or more treatment circuit plates discussed herein.Basic CD drive (for example, CD drive 140) provides various signals of deriving from CD.For example, most of CD drive provide HF signal 50 as basic information signal.This driver also provides tracking error signal (TE signal) 52, focus error signal (FE signal) 54 and come the baseband signal of self-sensing or laser photoelectricity detecting device, these signals often are referred to as a-signal, B signal, C signal simply, D signal, E signal and F signal.In the CD drive of known type, these signals are to handle on the treatment circuit plate so that any correction signal to be provided, and may need correction signal during at the operational light disk drive or from CD derived information signal.This treatment circuit plate often is encapsulated in the CD drive, and can comprise programmable signal processor discussed herein.

Fig. 9 A represents five kinds of embodiments of the present invention.These embodiments are designated embodiment I respectively, II, III, IV and V.Should be understood that every kind of embodiment can have several embodiment, all these embodiment realize purpose of the present invention.

In the embodiment of embodiment of the present invention V, untreated HF signal 50 is to extract out from CD drive 140, and is directed into remodeling personal computer or PC 142.Those skilled in the art will appreciate that remodeling PC 142 can be the processor of any appropriate reconstruction and programming, microprocessor, special IC (ASIC), or the like.Remodeling PC 142 comprises and is used for handling the software and hardware that produces the HF signal from CD drive 140 reading optical beams, some different layers in this beam modulation one-tenth dish 130, any one or a plurality of function of one or more investigation features in substrate or the surface.Running into or reading on the dish 130 during operating characteristics, identical reading optical beam also is modulated in a conventional manner.This operating characteristics generally includes hole and the platform in the prerecorded class CD dish, or dyes in such as the recordable disc of CD-R and be unstained and distinguish mark and the space that forms.According to any one scheme in a plurality of schemes of this information of coding, hole and platform, or mark and space comprise data, program, the coded message of video and/or audio character.

In remodeling PC 142, CD drive 140 preferably includes buffer amplifier (for the sake of clarity, not drawing) so that the en-largement print of HF signal to be provided, and therefore, actual HF signal can not distort because of excessive load.The en-largement print of HF signal (if desired, or actual HF signal itself) is coupled to the PC 142 that retrofits by cable.The software and hardware of handling the HF signal among the remodeling PC 142 comprises: analog to digital converter (ADC) is as the remodeling PC 142 of part, preferably, comprises data acquisition board or the module of suitable ADC (for example, from 8MHz to 40MHz sampling rate).ADC digitizing data from the sample survey stores among the RAM of PC, is handled by PC under the software control of PC.

Remodeling PC 142 can advantageously comprise: keyboard 144, monitor 146 and loudspeaker 148.After remodeling PC 142 handled original HF signal according to required mode, the characteristic aspect of investigation feature (following discussion) can show on monitor 146.Also can utilize the ordinary video or the sound signal of coding on monitor 146 and loudspeaker 148 display panels 130.For simple and clear, be " remodeling PC embodiment " to call embodiment of the present invention V in the following text.

In the embodiment of embodiment of the present invention III shown in Fig. 9 A, CD drive 140 is to be packaged together with the treatment circuit plate, this circuit board comprises: Programmable DSPs 32 (being similar to the DSP among Fig. 8) and analyzer 154, analyzer 154 combines operation with PC 158.PC 158 comprises software and is subjected to the hardware of software control, is suitable for the investigation feature is analyzed.Analyzer 154 can comprise: another Programmable DSPs, and programmable microprocessor, special IC (ASIC), or the like, be used to finish biological support, the function of chemistry or Biochemical Research.Programmable DSPs 32 comprises: the ADC of digitizing HF signal (or other signals).Analyzer 154 can only provide magnitude of voltage to surpass the counting of threshold value.Perhaps, analyzer 154 other waveforms that can discern change in voltage (for example, bimodal, or the like) or have the investigation feature.Under any circumstance, Programmable DSPs 32 is finished high bandwidth function, and PC 158 finishes the lowest-bandwidth function, and analyzer 154 finish in the middle of bandwidth capability.This area professional can understand that the function of analyzer 154 can be included in Programmable DSPs 32, in PC 158 or the limit of power of the two.This area professional can also understand that PC 158 can be the processor of any appropriate reconstruction and programming, microprocessor, and special IC (ASIC), or the like.The feature of this embodiment is below described in more detail.For simple and clear, be " DSP embodiment " to call embodiment of the present invention III in the following text.

According to the embodiment of embodiment of the present invention I, from the tap HF signal of driver 140, the signal that cushioned of tap impact damper 57 may be directed to the outside analog to digital converter 150 shown in Fig. 9 A (ADC 150) as shown in Figure 8.As the embodiment of remodeling PC, CD drive 140 preferably includes: buffer amplifier 152, be used to provide the en-largement print of actual HF signal, and therefore, actual HF signal can not distort because of excessive load.The en-largement print of actual HF signal (if desired, or HF signal itself) is coupled to ADC150.Perhaps, any one signal in various unlike signals or the signal combination (for example, TE signal and FE signal, or a-signal, B signal, C signal, D signal, E signal and F signal) can from shown in driver 140 extract out.Each feature of this embodiment is below described in more detail.For simple and clear, to call " A to the D embodiment " that this embodiment of the present invention is embodiment I in the following text.A to D embodiment can change over and comprise external buffer amplifier card 152, as the embodiment II among Fig. 9 A.

Refer again to Fig. 9 A, embodiment of the present invention IV explanation when the interrogating signal of driver runs into investigation feature or attribute, can utilize, and the audio frequency output (that is sound signal) that changes and expand CD drive 140 is to produce sound.For example, when the investigation feature " is read " or detected to reading optical beam, can utilize sonorific digital silence pre-recorded optical disc.In this manner, different investigation features can produce discernible alternative sounds or tone.Perhaps, CD can comprise soundtrack, it can get clogged encode sound information the investigation feature formation or exist interrupt.These embodiment of the present invention generally can be combined into three kinds of different classifications, method or technology.First method comprises utilizes existing audio cards, and it often is encapsulated in many actuator assemblies, for example, and audio CD player.This audio cards generally produces analoging sound signal, but the digital signal that also can be linked into sound is represented.Second method is to improve the voicefrequency circuit that exists in this current driver assembly internally, and the analog or digital signal can be provided.Provide external sound module (as the audio processing modules among Fig. 9 A 156) according to the third method of the present invention or technology, it is connected to CD drive assembly 140, process software, and audio output device, for example, a pair of loudspeaker 148.Embodiment of the present invention IV generally can be referred to as " audio frequency " embodiment.

All different embodiments among Fig. 9 A except remodeling PC embodiment V, generally include conventional PC 158, below for a more detailed description to its function.Remodeling PC embodiment comprises PC inherently, and changes its input.Yet, it will be understood by those skilled in the art that PC 158 can be the processor of any appropriate reconstruction and programming, microprocessor, special IC (ASIC), or the like.

The common U.S. Patent application No.09/421 that transfers the possession of, 870, its title is " TrackableOptical Discs with Concurrently Readable Analytic Material " (below be referred to as ' 870 application), the method of oscillograph to HF or RF signal openly is coupled, when obtaining the required coded message of operational light disk drive, detect bimodal figure with the investigation structurally associated.The appearance of these peak values is results that light beam passes reflectance varies when investigating structure or indicator on the optical disc surface.This electrical patterns can be advantageously used in and detect and differentiate the various structures of being studied.

The embodiment of embodiment II illustrates that modulus (A/D) converter 150 (ADC) are connected to the HF signal by impact damper 152.For example, embodiment II can determine bimodal number on the CD any part (and number of investigation structure or indicator).ADC 150 forms the digitized samples of simulation HF signals (or other appropriate signals), and forms sample with the high sampling rate that is enough to catch the peak value graphics feature, and these peak value graphics features are and investigate structurally associated.Amplitude and/or duration that relevant application program can be explained the digitizing peak signal, be used for determining relative size, composition and the position of detected structure.

Operating function

Operate feature collection from coiling 130 (Fig. 9 A and 12), reflection or the light that produces and handled by element in the objective lens unit 10 (Fig. 1) project on the detecting device 18, and the electric signal that generation servo circuit 34 (Fig. 6) is utilized is to provide operating function to driver.These patterns provide the tracking operating characteristics (for example, hole, groove, platform) that allows objective lens unit 10 to focus on the information on dish 130 focal planes and allow objective lens unit 10 to move along the code track surperficial relevant with the CD assembly operation.

Can utilize the operating function of driver and signal that bootstrap driver is finished these operating functions to reproduce multidimensional image.These operating functions include, but not limited to focus on, and follow the tracks of with synchronously.Interact and reflection and/or all energy sums of producing are referred to as HF (high frequency) signal or RF signal usually from the light of signal element and objective lens unit emission.If the photoelectric detector in the objective lens unit is combined into four-tuple (shown in 18 among Fig. 4), then this signal is referred to as the quaternary summing signal.This summing signal comprises by the required most information of algorithm process reproduced image.Other elements in the photoelectric detector produce can independent measurement signal to form additional information or operation signal.For example, the energy of collecting in can the quaternary detecting device of combination optical disc driver is to produce following operating function:

A+B+C+D=HF or quaternary summation (what be used to cheat is synchronous)

A+D-(B+C)=tracking (push-pull technique) (being used for the synchronous of groove)

A+C-(B+D)=focusing (astigmatism technology)

E-F=follows the tracks of (suspension technology)

Can independence or combination in any utilize these photodetector unit signals to produce the characteristic information of relevant signal element.In most of embodiment, need algorithm to interact in the software with the reproducing characteristic imaging.The quaternary detecting device is prevailing on current market, yet, can also utilize the detecting device that comprises more than 4 to 6 elements to distribute to strengthen characterization.In addition, the light path in this device can be configured to provide and have coherent light, the signal characteristic of partially coherent light or incoherent light.

Can from the operation signal of CD drive, collect signal response directly or indirectly.In an embodiment of imaging features of the present invention, operation signal is directly amplified, and digitizing or sampling (bit resolution, sampling rate) are carried out the algorithm adjustment to produce the characteristic image of signal element by software then.Before or after the digitizing operation signal, can also use these operation signals of electric treatment.Before digitizing, can utilize another signal element that operation signal is carried out filtering, amplification or summation are to produce characterization, and be nonrandom, or relevant response.For example, utilize the external electric operation,, can measure or the characterization signal as signal analyzer.Filtered when the response of signal, amplify, or with other signals (for example, asymmetry is recommended, cross-talk, noise radially, or the like) when carrying out mathematical combination, this signal can be showed nonrandom dependent event.

Can also independence or utilized in combination use the signal that produces from investigation feature NOR operation feature to produce the characteristic image of the investigation feature of being considered.Signal that CD drive produces and logical response also can be used for the characteristic of reproducing signal element or investigation feature.This is with to utilize signal to finish the action required characteristic different slightly.Can collect digital signal from CD drive, simulating signal, logical response, photoresponse, or mechanical response is to describe signal element or investigation feature.For example, signal element and light with particular optical or physical property interact, and set up characteristic energy figure or energy distribution.Can monitor with measuring photodetector on this characteristic distribution or figure in response, it can not produce adverse influence to the operating function of driver.

In a typical embodiment, the operating characteristics on objective lens unit in the CD drive and the CD assembly surface interacts.This interaction can comprise supports all operating functions, or it can be included in one or more operating functions of predetermined " subregion " last time-out of dish.At the HF signal, produce signal response in tracking signal and the focus signal from the interaction of the incident beam of objective lens unit and operating surface and investigation feature.Comprise operation information and can be used in combination with electric servo circuit, to support on certain direction that to the response of objective lens unit motion, we claim that this direction is " Z " direction, shown in Fig. 9 B with the focus signal of operating characteristics relevant information.Tracking signal or push-pull signal can be used in combination with electric servo circuit, and with the response of supporting on certain direction objective lens unit to be moved, we claim that this direction is " X " direction, shown in Fig. 9 B.HF signal, DPD signal or quaternary summing signal can be used for supporting on certain direction that to the response of objective lens unit motion, we claim that this direction is " Y " direction.According to the method, utilize the standard operation response of objective lens unit, we can collect 3 dimension (XYZ) responses from the interaction of incident beam and signal element or investigation feature.

The zone that signal element or investigation feature 136 cover in the CD, it interacts with the operating characteristics that tracking signal is provided directly or indirectly.Signal element can be away from operating characteristics, for example, and in the transmission-type or semi-reflection-type CD of following detailed description.Perhaps, signal element can remove from the zone that the complete operation function is arranged, and for example, in CD or independent " subregion " or " the catoptron band " that form on the CD, has wherein removed the certain operations function at least.Signal element can be on the focal plane, and it is actual removing from the conoscope image that produces other operation signals (focusing on and/or tracking and/or synchronous).Signal element should provide measurable contrast or energy distribution.This contrast can be determined by the difference of the reflectivity properties of the reflectivity properties of operating structure (focal plane) and signal element.

The reflectivity of signal element or investigation feature can be less than the reflectivity of operation planar, thus to photoelectric detector provide the energy that reduces and.Signal element can have and the identical or similar reflectivity properties of operation planar (or focal plane), but provides diffraction or phase cancellation or phase place to strengthen response, and it provides the signal level that reduces in photoelectric detector.Therefore the reflectivity of signal element can provide the signal level of increase greater than the reflectivity of operation planar in the photoelectric detector summing signal.The design that can strengthen the design of CD drive and CD assembly operation feature is to optimize imaging capability.

The interaction that provides the operation planar of focal plane can be designed to provide maximum-contrast or strengthen laser/signal element.This area professional can understand, any special time during laser focusing, and the focal plane comprises maximum reflectivity point.If the reflectivity of focal plane is higher than signal element or investigation feature, then need to strengthen the interference or the phase contrast characteristic of design.If the reflectivity of focal plane is lower than signal element, then need the reflectivity and the linear signal response of enhancing signal.Focus resolution depends on the optical maser wavelength in the objective lens unit, the numerical aperture of condenser lens, the optical property of the bandwidth of focus servo loop and CD assembly.

In according to optical disk system design of the present invention, importantly set up strong signal recognition mode, it can distinguish operating function and signal element characteristic.By CD reflection, absorb or the light of transmission should be as far as possible in detail and how to describe signal element.

Signal element by many methods can influence the reflection or transmitted light in response.Signal element itself also can be designed to have the operating characteristic of dish, thereby high contrast is provided in transmission or reflected light.The energy level of light, energy distribution and polarization state can be subjected to the influence of focal plane optical property relation in the design of signal element and it and the CD.

An embodiment who relates to signal element selection and design sets up such signal element, and the reflectivity of its reflectivity and CD focal plane has a great difference.Therefore, the reflectivity of signal element is designed to be higher or lower than the reflectivity of CD far away.This just produces high signal contrast and strong characteristic signal.

Can produce reflection differences between focal plane and the signal element according to several different methods.These methods include, but not limited to following content:

1. the material of signal element can have lower or higher reflectivity, and therefore, it produces higher or lower signal level on every side than disc plane.

2. if signal element less than beam sizes, then produces conoscope image, and the size of signal element can be set up to signal and disappears mutually or the component of addition.

3. the energy source of setting up status condition can the activation signal element, and this status condition causes than the lower or higher reflectivity in peripheral region (phase-change material).

Relate to that signal element is selected and another embodiment of design relates to the utilization of material and state, it interferes with polarization state by CD transmission or reflection.Signal element can be designed to optics and magnetic property, and it is for the interactional polarization state of CD assembly the contrast effect being arranged.In CD, set up this embodiment many kinds of methods are arranged.These methods include, but not limited to following content:

1. signal element can be designed to transparent rather than birefringence or bitintability.Optical element reflects or absorbs the signal that forms based on its state transmission in the detecting device.

2. signal element can be designed to produce magnetic orientation, and it provides the contrast polarization state of light.

Relate to that signal element is selected and the 3rd group of embodiment of design relates to the utilization of optics or interventionist nature, it interferes with transmission or the light energy distribution that reflects by CD.Signal element can be designed to according to this sample loading mode diffraction light, and it sets up higher energy response from extrinsic detecting device.In the formation signal in detector surface glazing zone, provide and to measure or detectable contrast.

Relate to that signal element is selected and the 4th group of embodiment of design relates to and utilize chemiluminescence.Signal element or optical disc surface can be designed to launch secondary activating attitude energy.For example, it relates to utilizing and is added to fluorescence or phosphorescence mark or dyestuff on signal element and/or the appropriate light panel surface.The appropriate signals element of this group embodiment includes, but not limited to pearl or cell, as haemocyte.State is activated by the projectile energy on the CD assembly.Thereby set up specific energy, it is present in the back light of transmission or reflection.

Therefore, the objective of the invention is a kind of optical analysis disc that is used for the detection signal element.Optical analysis disc preferably includes: substrate layer and the operation layer relevant with substrate layer.Operation layer has the operation information of coding.According to this aspect of the present invention, optical analysis disc also comprises: with respect to the signal element of operation layer placement.Signal element and operation layer have optics and the magnetism characteristic of choosing, and the degree of the predetermined comparison between them can be provided, thereby the return signal of distinguishing between the characteristic of pointing out information that operation layer is relevant and signal element is provided.

Incident light

The optical wavelength of utilizing laser instrument (that is the light source 19 among Fig. 1 and 12) to launch in the objective lens unit 10 of CD drive shown in Figure 1 influences the resolution of optical disk system.In theory, utilizing wavelength is λ/2NA for minimum signal element or the investigation feature that the objective lens unit (at element/laser interface) of λ can detect, and wherein NA is the numerical aperture of object lens.At any time, investigation feature or the signal element at laser/planar interface place can be greater than or less than near the scope that light beam covers focus.With less than the signal element of laser coverage relatively, greater than laser/signal element at the interface the signal element that covers of laser beam produce more characteristic signal.

Wavelength of Laser should be lacked as much as possible to improve the resolution of signal response.This can cause less signal element and investigation feature to have more detailed and feature capabilities.Short wavelength produces less focal beam spot on the focal plane of CD assembly.So short wavelength produces higher spatial resolution in imaging device.Objective lens unit with high-NA provides less focal length and bigger diffracting power.

Driver should have very little machinery or film to ring to the signal response or the signal conformance at laser/signal element interface.In some cases, the driver operation can exert an influence, if it is design like this (for example, electric coupling strengthens signal gain control setting AGC, signal power level setting synchronously).

From the CD information extraction

Referring now to Figure 10, Figure 10 explanation encoded video on CD, data or audio-frequency information, for example, music, then from the CD decoded signal to recover audio frequency, video, or the conventional process of data.At square frame 70 sampling audio frequency, video, or data-signal quantize at square frame 71, are encoded into standard format at square frame 72, and are modulated on the premaster at square frame 73.Then, make replicating disk in square frame 74 amounts of draping over one's shoulders.At square frame 75, the signal that in disc reader, obtains from the manufacturing dish by signal processor processes.In square frame 76 demodulation, decode to recover original audio frequency, video, and/or data at square frame 77 then.This output is presented on the monitor and/or on a pair of loudspeaker and plays, and uses and appreciates for the user.

Secondly with reference to Figure 11, Figure 11 shows the CD decode system after the variation, wherein gets rid of some functions in the system.Change the path with removal demodulation code operations (

square frame

76 and 77 among Figure 10), and provide original digitized signal, can describe one group of signal element on the CD assembly surface effectively or investigate feature to computing machine.

Except prerecorded CD, current also have various recordable CDs.Recordable premaster comprises various operating characteristicses, and these operating characteristicses are designed for during the recording operation, rather than are used for during the read operation.Following table 1 is summed up the difference of these operations.

Table 1

Disc-type	Function	Record character	Read or play character
Disc-type	Function	Record character	Read or play character	????CD-R ????CD-RW	Focus on	Surface nature	Surface nature
Follow the tracks of	22.05kHz swinging chute	Hole or mark			Focus on	Surface nature	Surface nature
Follow the tracks of	22.05kHz swinging chute	Hole or mark	(speed control) synchronously		22.05kHz swinging chute	Hole figure (mark)
????DVD-R， ????DVD-RW ????DVD-RAM ????DVD+RW	Focus on	Surface nature	(speed control) synchronously		22.05kHz swinging chute	Hole figure (mark)	Surface nature
	Focus on	Surface nature	Follow the tracks of	The 140/160kHz swinging chute	Hole or mark		Surface nature
	(speed control) synchronously	The 140/160kHz swinging chute	Follow the tracks of	The 140/160kHz swinging chute	Hole or mark	Hole figure (mark)

As already pointed out, CD, the demoder of CD-R or DVD player or servo-drive system can be used for providing the counting of chemistry response on the focal plane of dish or the operating characteristics plane, and be relevant, or feature.Other responses or original signal from the driver chip group, this chipset quantizes the signal amplitude of investigation feature or signal generation element, comprise: high-frequency signal (HF) (AC or DC coupling), tracking error signal (TE), focus error signal (FE), automatic gain control setting (AGC), recommend tracking signal ((B+C)-(A+D)), CD tracking signal (E-F), CD-R tracking signal ((A+D)-(B+C)), focus signal ((A+C)-(B+D)), differential phase detection device signal (DPD) ((A+B)-(C+D)), from the power monitoring signal at laser instrument rear portion, and sound signal.Utilizable other signals of the present invention comprise: from quaternary detecting device A, and B, C and D, or each signal of side detecting device E and F.

The trend of current conventional driver is to utilize the high density photoelectric detector to replace typical quaternary detecting device.Method of the present invention also can be used in combination this array detector that can buy in the market.For example, these arrays comprise each signal A, B, and C, D, E, F, G ... Z.According to different embodiments of the invention, can advantageously utilize each signal in these signals or its combination obtaining required electrical patterns, signature, or signal disturbance, they describe interested signal element uniquely, investigate feature or attribute.

Imaging technique and operating characteristics

The operating characteristics and the layout of photo bio dish assembly can be designed to optimize imaging operation.The CD assembly can also design in specific regional or " subregion " detection and the characteristic with enhancing signal element or investigation feature, and the resolution of the higher signal with specific laser interfacial property is provided.

Operating characteristics often provides such tracking signal, and it allows objective lens unit to move to outskirt from the inner region of CD assembly.Track pitch or interval less between the operating characteristics produce higher resolution, owing to correspondingly increased and the relevant number of responses of investigation feature.By the operating characteristics less than signal element and the track pitch suitable with signal element is provided, can from interacting, laser/signal element collect repeatedly electric scanning.When track pitch reduces, can increase near the potential scanning times of last or place, the fixed position signal element of operation planar (focal plane).When operating characteristics during, just increase investigation feature or the distinguishable degree of accuracy in signal element position on the CD less than given fixed signal element size.This position precision generally is referred to as " position resolution ".This is equally applicable to consistent object lens/operating characteristics interface.The typical track pitch of CD dish is 1.5-1.7 μ m, and the track pitch of DVD dish is 0.73-0.75 μ m, and the track pitch of DVD-RAM dish is 0.35 μ m.

Operating characteristics also provides influences disc spin speed and laser and the synchronizing information of signal element interaction duration.In A/D converter signal digitalized, produce higher quantization resolution than low velocity.This increases the sampling resolution of system.When rotational speed increases, must increase sampling frequency and bit resolution to obtain consistent sampling resolution according to corresponding manner.The typical magnetic track speed of CD dish is 1.2m/sec, and the magnetic track speed of DVD dish is 3.49m/sec.

Operating characteristics also provides influences CD speed and laser and the synchronizing information of signal element interaction duration.Lower rotating speed produces higher quantization resolution in A/D converter signal digitalized.This increases the sampling resolution of system.When rotating speed increases, must increase sampling frequency and bit resolution according to the mode of correspondence to obtain consistent sampling resolution.The typical magnetic track speed of CD dish is 1.2m/sec, and the magnetic track speed of DVD dish is 3.49m/sec.

The CD assembly of CD assembly or part can specific design become to be exclusively used in imaging operation.Have low-rotate speed, the CD of close magnetic track pitch and optimization focal plane is provided for the exception response of imaging.The design of optimization dish or disc elements also comprises logical circuit, can be linked into the investigation position that dish is gone up pre-addressing at random.Logical circuit can be coded in the operating characteristics of dish, or is formed by the physical markings on the dish assembly.Logical circuit can be designed to be adapted to be assigned to the concrete mensuration investigation agreement that dish is gone up different subregions.This investigation agreement includes, but are not limited to, sampling rate, and bit resolution, rotating speed focuses on and follows the tracks of skew, laser power, optical maser wavelength, sense of rotation, acceleration, retarded velocity and concrete mensuration require any other system interaction.

Can set up such optical disk system, have (1) operating characteristics or tracking characteristics, it has track pitch very closely to realize collecting the tangent resolution (for example, DVD-0.74 μ m) of data; (2) logical circuit and hardware, it provides platform/groove or platform/hole to follow the tracks of, and strengthens tangent resolution (for example, DVD-RAM, or MO); (3) logical circuit or operating characteristics, it provides slower dish rotating speed (short hole or swinging chute) to obtain the higher signal details; (4) dish or lens element thickness, it can have less or bigger hot spot (can be departed from correct focal plane slightly by the element of imaging, or they can provide the new focal position of objective lens unit) on the focal plane of dish assembly; (5) hardware, it provides the laser of shorter wavelength to improve resolution (for example, DVD 635-650nm, the about 400nm of HD-DVD); (6) sampling system, it provides higher sampling frequency or higher sample bit resolution to operate with Enhanced Imaging; (7) software, it provides the processing capacity exclusive disjunction function of mathematic(al) manipulation approximate to export to picture; (8) dish or lens element make laser and feature vicinity, can strengthen the interaction (for example, being used to control the SIL type element of evanscent field) of light and feature.

On the photo bio dish, in the imaging operation of investigation feature, need between investigation feature or signal element and focal plane, produce measurable contrast.Utilize signal element or investigation feature and the focal plane of antiradar reflectivity or the combination of operation planar of reflection, can obtain this required contrast.Perhaps, utilize signal element or investigation feature and the focal plane of high reflectance or the combination of operation planar of non-reflection, also can obtain this required contrast.

CD drive and relevant dish form

Comprising such as CD, CD-R, or can realize the photo bio dish on the CD of DVD or its remodeling form.The photo bio dish can comprise and be used to finish, control and aftertreatment test or the coded message of measuring.For example, can guide the rotating speed of this coded message with console panel.With test, measure or the investigation agreement relevant, rotating speed can be with acceleration, the intervention of constant speed and retarded velocity or session and changing continuously.Can control the speed of these sessions tightly, direction and rotation time so that the predetermined mix of fluid to be provided, stir or separation, and reagent, the interruption of reaction reagent or antibody.Disk drive component is used for rotating disc, reads and handle any coded message of storing on the CD, and liquid in any mensuration subregion of optical analysis discs, chemistry, biology, or biochemical component.Disk drive component also can be used for writing information to biological dish.Record carries out before or after can occurring in and finishing mensuration or test.

According to an alternative embodiment of the invention, with the sampling reading optical beam, after this identification investigation feature and structure are described this feature and structure as unique signal disturbance or electronic signature to the CD drive chipset again as A/D converter.Can change the CD decode system according to this sample loading mode, remove some function in this system.From the decoding path of CD demoder, remove concrete feature so that original digital signal to be provided, it can be described effectively and discern on the photo bio panel surface uniquely, the investigation feature of placing on the substrate in the CD, or reside in the investigation feature that is formed in container or the passage fluid element as the dish assembly.

According to an alternative embodiment of the invention, conventional disk drive is used for identification investigation feature and structure.In the case, the change of firmware can make the known signal in user's monitor disk driver, need not change circuit or hardware.The value of AGC signal can be used as survey instrument.The AGC function attempts to guarantee that analog output signal has consistent scope.If disk drive is used to read binary data, then only need high value and low value.Yet, under the situation of investigation feature, may need the numerical value in the successive range.When signal level was low level, AGC was high value, and vice versa.Therefore, AGC can be used as such signal, and the light that on behalf of detecting device, it receive is so AGC can be used for measuring and detecting the variation of investigation feature.

The CD-R player/recorder of this embodiment is added the objective lens unit of adjustment lens to the commercialization CD player.Commercialization player/recorder meter becomes to be used for to write down the record of CD dish, utilizes it to detect microstructure now.Outgoing position at objective lens unit changes driver.Little refractive optical lens adds on the light path that CD can write down driver.Figure 89 A and 89B draw and add this adjustment lens.Figure 89 A represents to have the commercialization CD player that can write down CD dish 1112.The layer of polycarbonate that 1.2mm is thick is represented in the shadow region, and it helps to focus on and the polarization light beam.In Figure 89 B, adjust the outgoing position that lens 1114 are added on objective lens unit 1100.This light is adjusted lens required focusing and polarization characteristic is provided, be used for CD can write down driver standard operation.Adjusting lens adjusts focal path and provides required reflection to diode laser, so that form desirable spot size and energy distribution on optical disc surface.Adjust lens the polarization phase-shifting that is similar to the thick layer of polycarbonate formation of 1.2mm polarization phase-shifting in the compact disk structure is provided.There is not this layer of polycarbonate in the used CD 1116 of present embodiment.The substitute is, the air packed layer is used to deposit the biological specimen that detects and analyze.Can change adjust lens characteristic to be provided for the optimal cases of detection of biological position, for example, pearl, cell, aurosol, carbon, or other micro-mark and the indicator relevant with CD.The operating characteristic of lens with each element on the light path of optimizing CD player/register adjusted in design in such a manner.

Sampling system designs and is optimized to and detect and describe electroresponse from lip-deep investigation structure of optical analysis disc and signal element.The information from servo-control signal discussed above is monitored and transmitted to sampling system.The information of transmitting comprises qualitative and quantitative information.

The objective lens unit of CD player or register is sent out from the modulation of laser diode or continuous wave laser pulse.The lip-deep reflective information of its recording disk is to the combination photoelectric detecting device, and generation provides four servosignals (that is, follow the tracks of, focus on, synchronous and power control) of operation requirement.Can detect and describe microstructure each electric signal that produces from electric servo or its combination.This includes, but not limited to utilize in three light beam suspension (outrigger) systems all to follow the tracks of hot spots to detect and to describe feature.

At least utilize three kinds of focusing technologies can produce focus servo signal: critical angle focuses on, and the Focault or the edge of a knife focus on, or astigmatic focusing.At least utilize four types of tracking techniques can produce tracking servo signal: i-beam push-pull is followed the tracks of, and three light beam suspensions are followed the tracks of, differential phase detection (DVD), or the single beam swing in high frequency is followed the tracks of.At least utilize three kinds of diverse ways to produce synchronously: bit clock is synchronous or bit pattern is synchronous, subregion timing method (DVD-RAM), or swinging chute is synchronous.Utilization produces power control more than four kinds of methods: the power monitoring signal during PCA or CD-R coil, and the realtime power control of operate power control method or pulse diode, power or strategy are adjusted, or the best power of encoding in the swinging chute information.From more than producing logic 40 known optical disc forms.Logic can detect the completing place, and power control radially or is tangentially located, and layer detects, Density Detection, many conversation applications and various other functions.

The disc drives servo control mechanism can be used for detecting investigation feature, signal element, or the size of its various structures.The motion of laser on biological position causes the signal deflection in CD tracker servosignal and/or the CD focusing system servosignal.Recommend the relevant electric signal deflection of tracker or three light beam suspension trackers with the closed signal light beam and be used to describe biological position, and can be processed into quantitative numeral or analog information.The electric signal deflection relevant with closed loop three light beam astigmatisms or closed loop single beam Focault focusing system can be used for describing biological position, and can be processed into quantitative analog or digital information.

When recommending the interaction of tracker and spherical biological position, optical assembly is along level and perpendicular planar movement.Optical assembly is towards outside or radially inside along moving of horizontal plane.This causes being added to the positive deflection or the negative bias of recommending the tracking servo circuit power on signal changes.Low pass or band energising wave filter can be used for determining the existence and the characteristic of biological position.

When three light beam suspension trackers and the interaction of spherical biological position, optical assembly is along level and perpendicular planar movement.Optical assembly is towards outside or radially inside along moving of horizontal plane.This positive deflection or negative bias that causes being added to differential tracking servo circuit power on signal changes.Low pass or band energising wave filter can be used for determining the existence and the characteristic of biological position.

The circuit of isolating with tracking servo loop electricity can be determined the difference of biological position characteristic.This circuit can be a series of low passes or band energising wave filter, and they are used for isolating and describing the frequency and the amplitude of closed loop tracking system deflection, and deflection is because the motion of laser on biological position causes.The size of biological position can be described with the deflection amplitude of recommending electric tracing signal in tracker or the suspension differential tracker.The size of biological position and shape also can with recommend or the differential tracker in the deflection frequency of electric tracing signal describe.

In other embodiments of the invention, the CD assembly of multiple beam is read from multilayer disc.One deck/light beam is to providing such as the operation element of following the tracks of.Meanwhile, remaining layer/light beam is to being used for the detection of biological position.

The Control Driver function

For optical disk system is correctly moved, it is necessary: (1) focuses on the operation planar of CD assembly exactly; (2) follow the tracks of spiral fashion disk track or on panel surface, utilize the even radial motion of certain form exactly; (3) information that recovery is enough is to realize the form (CAV, CLV, or VBR) of speed control; (4) utilize logical message of from dish, collecting or the signal graph that detects at the dish operation planar, keep correct power control; (5) in response to the position of controlling objective lens unit, rotating speed, or the logical message of laser focus position, this information is responsible for providing the operation requirement.

Utilize circuit and logic servo control mechanism, the CD objective lens unit is finished three main operation requirements.Therefore, objective lens unit provides electric signal to (1) focus servo circuit, (2) tracking servo circuit and (3) information-processing circuit.But under the situation of CD register system, it is essential that the 4th requirement of power control is provided.In these systems, objective lens unit also provides electric signal to laser power control circuit (" signal monitor ").

When CD can write down (CD-R) but dish when in CD recording played machine, playing, its utilizes " swinging chute continuously " and reflection-type panel surface providing information to focus servo mechanism, tracking servo and power-control servo.Before writing recordable disc and the contrast mark is provided, on optical disc surface, detect less than feature.Quaternary summation detecting device detects optical contrast ratio structure on the air plane of incidence that is placed on inverse imaging CD recordable disc.The characteristic signal that these structures provide the pyroelectric monitor operation of quaternary summation detecting device to detect.

Swinging signal is used for satisfying above operation and requires #2 and #3.The swinging signal component that is referred to as secondary swing (two-phase mark) is responsible for operation and is required #5.

In the tracking of light objective lens unit and HF signal, can detect hole or marker graphic.On the sagittal plane, do not produce signal.But utilize the CD register system, the tracer request of the electrical effect of biological position and system is isolated.Produce the recordable swinging signal of CD on the sagittal plane, it can not be detected in the HF servosignal.The main sagittal plane of detecting in tracking signal.In the HF signal, can detect little information from radial component.This can make us that tracking signal and HF signal or quaternary summing signal are isolated.The application that sort signal is isolated for the detection of biological position is favourable.

Utilize following mathematical relation, can describe the biological position effect on the swinging signal.But in current CD register system, utilize the swinging signal of the locking frequency of 22.05kHz or 45.35msecs.On-line velocity is under the situation of 1.2meters/sec, and we relatively swing the single ripple of figure and 54.42 microns physical components.In other words, we can detect very large physical features on the sagittal plane.

Utilize this isolation technology, we can not lose the very large feature of detection under the tracking condition.By monitoring HF signal and tracking signal simultaneously, can determine the successful physical measurement of biological position.If biological position is sufficiently little, then can finds electric deflection, and not have relevant electric deflection at the output terminal of tracking signal at the output terminal of quaternary summing signal or HF signal.Certainly, on tracking signal, can find some electric pulses, but comparatively speaking it is in the extreme little.Similar relation is also arranged between HF signal and focus servo signal.

Referring now to Figure 12, Figure 12 is the zoomed-in view of embodiment II (Fig. 9 A), and it shows to have biological position, the CD assembly 130 of signal element or investigation feature 136 and combination be CD drive 140 implemented according to the invention, buffer amplifier card 152, ADC 150, PC 158 and display 146.In one embodiment, extract original detection signal (A, B, C, D, E and F) out, and they directly are fed into external buffer amplifier card 152.In another embodiment, before entering into external buffer amplifier card 152, the signal A of detection, B, C, D, E and F handle in the driving impact damper 151 of CD drive.In another embodiment, the signal of the original signal of extraction and 151 processing of driving impact damper all is fed into external buffer amplifier card 152.According to embodiment of the present invention II (Fig. 9), the signal of external buffer amplifier card 152 outputs enters into ADC 150 and further processes.

Continuation is provided for drive motor 95 and controller 96 that console panel 130 rotates with reference to Figure 12.Can utilize hardware trigger sensor 141.Triggering sensor 141 provides signal to ADC150, only is collections of permission data on target area 135 time when incident beam 137.Photo bio dish 130 comprises by triggering the triggered mark 166 that sensor 141 is read, and triggers sensor 141 and presents trigger pip to capture trigger card 167.Capture trigger card 167 preferably but needs not to be, and implements on impact damper card 152.Triggering sensor 141 can be positioned at the bottom of CD assembly 130.This system can also comprise the top detector 160 that is used to detect transmitted light 162.This transmitted light can transmit by the semi-reflection-type dish, or by removing the subregion of CD reflection layer.Below discuss other features that are applicable to various types of disc of the present invention in more detail.

Figure 13 represents to have dish 130 plan views of target area 135 and triggered mark 166.Hardware trigger mark 166 is preferably disposed on the outward flange of dish, and preferably on the radial alignment that target area 135 is arranged.

Capture trigger card 167 provides points out when triggered mark 166 has arrived the signal with respect to investigation feature 136 precalculated positions.PC 158 is handled and entered to this signal by ADC 150, is used for occurring in synchronously the processing of PC 158 and the position of triggered mark 166.For example, triggered mark 166 just in time is placed on and comprises the position before the sector in the biology dish 130 of investigating structure.When PC 158 detected triggered mark 166, PC 158 waited for the schedule time of a weak point, and the signal conduct that begins then to handle from HF signal extraction points out to exist the data of investigating feature.Meanwhile, when triggered mark 166 was detected by PC 158, PC 158 set the timer of the longer schedule time, and PC 158 handles the operation information that is used for the operational light disk drive from the signal conduct of HF signal extraction more after this.

Figure 14 is the block scheme of relation between PC 158 and the CD drive 140.Embodiment according to embodiment of the present invention II (Fig. 9 A) comprises top detector 160, and the additional drives functional block that triggers sensor 141 and treatment circuit is preferably on single printed circuit board (TAD) 82.Therefore, this functional block detects transmitted light and triggered mark, and based on the transmitted light that detects, amplifies analog data signal.These additional functions are preferably made such, and it need not change existing CD drive circuit.So, initially transport or utilize additional function remodeling before need not change existing hardware, can change conventional CD drive.

According to an alternative embodiment of the invention, and select, can in service data, provide trigger pip 83, the position that makes the coded message on the dish point out to investigate feature as the another kind of triggered mark 166.In another embodiment, read whole dish, but only keep the data after the tentation data group.According to the method, at first all data on the dish are read in the storer, abandon software trigger data before then.Randomly, can provide second triggered mark.This second triggered mark can be used for distinguishing a plurality of target areas, but can make the user check interested specific region.Have a plurality of triggered mark of corresponding detection trigger device if utilize, then each triggered mark must be on different radiuses.

ADC 150 can also receive the analog driver signal through buffer amplifier card 152, and buffer amplifier card 152 receives its input signal from CD-ROM driver 140.In PC 158, cpu motherboard 87 is communicated by letter with CD drive 140 by small computer system interface (SCSI) 88, and receives data by expansion bus from ADC 150.Cpu motherboard 87 has ethernet connector 88, and it allows this data unloading to be for further processing.The input of power source 89 received powers also provides power to other elements among cpu motherboard 87 and CD drive shell and the PC 158.

Can handle the data of collecting according to real-time mode, or data can store and do aftertreatment by other computing machine, it can reduce the complicacy of system.

Trigger, amplifier, detecting device card TAD 82 preferably has such structure, and it can be installed in the conventional CD drive, can be used in the drive floor in the computing machine.A kind of driver that is particularly suitable for developing purpose is Plextor model 8220 CD-R drivers.Though can utilize CD or DVD driver, the CD-R driver has several useful features.Because the CD-R driver can be read and write-in functions, laser instrument can be operated in higher power level range.This function of utilizing higher-wattage is useful for the investigation feature of some type.Another useful feature of CD-R is that it has the ability that is written to dish, gets back on the dish so can be used for writing the result.This just allows the result is kept at and is provided with the back on the dish and uses and preserve with dish.

Figure 15 is TAD 82 top views that comprise the detection trigger assembly according to another aspect of the present invention.This circuit board comprises a perforate or through hole 80, when utilizing the top detector drive unit of transmission-type dish, need this through hole 80, for example, in the common U.S. Patent No. of transferring the possession of 5,892, in the 577 disclosed devices, its title is " Apparatus and Methods forCarrying Out Analysis of Samples ", it is for reference to be incorporated in this, and at U.S. Provisional Application No.60/247, in the 465 disclosed devices, its title is " Disc Drive for OpticalBio-Disc ".When the conventional driver that utilizes the reflection-type dish and vicinity that is provided with usually or floor detection device, just do not need through hole 80.As discussing in conjunction with Figure 12, TAD 82 comprises: detection trigger device 141 and detecting device 160.In this specific embodiment, use three detecting devices 160.

Figure 16 is the circuit diagram of trigger circuit shown in Figure 15.In order to obtain the information of relevant investigation structure, be equipped with suitable trigger circuit according to CD drive of the present invention, it is to implement trigger action when needs detect untreated HF signal 50.This is essential, because the signal Processing that DSP32 (Fig. 8) finishes, it generally includes demodulation, and decoding and error checking are attempted the EFM coded message on the HF signal 50 is converted to specific digital format.Though provide the CD of operation information partly to produce digital format information, investigation feature of the present invention does not produce the EFM coded message.The HF signal of handling according to decoding EFM coded message mode can not easily be used to detect and investigate the bimodal of structurally associated.Therefore, before the DSP that arrives CD-ROM driver, extract interested signal out, and triggered mark 166 and the trigger circuit shown in Figure 14 and 26 are implemented according to the method described above.

Figure 17 is the block scheme that illustrates in greater detail mutual relationship between TAD 82 and the CD driving mechanism.As shown in figure 17, optical element 92 is installed on the carriage assembly 172 that is driven by tray motor 94, and CD is to be driven by cd-rom motor 95.Carriage assembly 172 comprises: optical pick-up unit (OPU).Drive two motors from the controller 96 of CPU 87 received signals.From the data of optical element 92, detection trigger device signal 83 and all offer TAD 82 from the signal of transmission-type (top) detecting device 160 or detector array.The detecting device that is used to handle transmission or reflected light beam signal can be single detector unit or radially or edge a plurality of cell arrays of arranging, and also can be placed on the dish relative with laser one side, and can directly be installed to TAD or install dividually.

ADC 150 can randomly be placed on the sampling card of unusual high-speed transitions.A kind of spendable card is Ultrad 1280 DX, and it has two 12 A/D converters, and its sampling rate is up to 4,000 ten thousand samples of per second.

It is favourable that disk drive is done to change, and minimum interruption is provided can for conventional driver.Therefore, it is desirable to use the transmission-type dish.In other words, the service data seen for active circuit of this dish and the driven function of generation are enough reflections.But it remains the part transmission, allows some incident lights transmission to arrive top detector by dish.In this manner, the investigation feature can be detected and catoptrical detector circuit need not be changed.Reflected light still can be used for reading coded data.

Refer again to Figure 18, the TAD 82 shown in the functional block can comprise transmission-type or top detector 160, and it is positioned at observes on the district or through hole 80 shown in passing.This detecting device can be a single detector, and the array of the different segmentations of radial array is arranged, or the array of a plurality of segmentations of arranging along the edge, and wherein a plurality of detecting devices are arranged along different radiuses.Detector cell 160 received signals, and they are offered prime amplifier 120, automatic gain control 122, switch 124 and amplifier 126 are to produce the signal of 3 volts of magnitudes.

Triggering light source and detecting device 141 can be configured on the TAD 82.This hardware comprises light source and is used to detect the detecting device of triggered mark, is preferably disposed on the edge of dish 130.In this specific embodiment, configuration second triggers light source and detecting device 128 helps to distinguish a plurality of triggered mark.In the case, these two trigger pips are all on trigger control circuit.Trigger control circuit transmission trigger pip is to collect and to keep from required sample area to ADC 150 data.

If data detector is to have a plurality of cellular arraies, then can utilize analog switch 124.A plurality of detector cells can be arranged, and they finish the refract light combination of some type.For example, can utilize and value and difference.If desired, switch can be coupled to the detector cell that is used for detection of reflected light under the CD.This can make system obtain that the top is detected and floor detection between difference.

On TAD 82, can dispose additional processing and tally function,, or replace ADC 150 and PC 158 (Fig. 9 A) effectively, allow on TAD 82, to take place more processing operation in order that remove the processing operation from ADC150.For example, under the situation of test CD4/CD8, a kind of method is to calculate the number of white blood corpuscle in the target area.At the common U.S. Patent application sequence No.09/988 that transfers the possession of, this method is disclosed in 728, its title is " Methods andApparatus for Detecting and Quantifying Lymphocytes with OpticalBiodiscs ", application on November 16 calendar year 2001.When laser is being measured when scanning in the district, detecting device less than light, and detects complete light in the heart the time in the haemocyte rim detection in haemocyte.Because light beam scans, so it produces a series of height and low signals of pointing out detected cell.Can add processing capacity goes up to comprise threshold crossings circuit sum counter to card.This processing operation is more simple to operate than the processing that may use in other tests.Every kind of circuit in these type circuit is well-known.The test-types (CD4/CD8 is an example) that depends on use, disposal system may need on analysis of accounts district or the target area hundreds of or up to several ten thousand features.In addition, can also add microprocessor to card.

By providing additional processing and/or tally function to card, can be directly through USB port or by ethernet port from blocking the result that scanned samples is provided.Utilize Ethernet, can provide data, make the family can utilize the web browser to insert data from the web server.

TAD 82 can also comprise temperature sensor (not shown) and other sensors that can be used for testing.Under the situation of temperature sensor, test can utilize relative temperature to point out existing of some material.The another kind of detecting device that can provide is simple bar code reader, if on the dish bar code is arranged, then bar code reader can be discerned test.

Automatic gain control (AGC) 122 and automatic electric-level control (not shown) guarantee to utilize whole dynamic range, and for example, signal can be in 0 volt to 3 volts scope.Automatic electric-level control (ALC) is used for determining the center of signal, for example, 1.5 volts, if the scope of signal is 0 volt to 3 volts.The amplification result of AGC and ALC is the passing threshold processing of circuit to export and to provide consistent result.

As an object lesson of Fig. 9 A and 12 illustrated embodiments, driver of the present invention comprises known CD drive, and it has been retrofit into and has comprised capture trigger card 164, impact damper 152 and ADC 150.For example, driver of the present invention and optical disc apparatus are sold to the research laboratory.Optical disc apparatus comprises drive software, and is a kind of CD of known type.The research laboratory connects driver of the present invention and arrives known PC (for example, PC 158) and move optical disc apparatus so that drive control software to be installed, and it can make PC 158 and driver of the present invention move as research equipment.So all kinds photo bio dish comprises triggered mark 166 and target area 135, is sold to the research laboratory that can carry out various researchs and mensuration.Biological dish 130 can comprise miniflow in target area 135 and the sector and the coded message in another sector.Coded message comprises operation information that is used for the operational light disk drive and the data that comprise relevant test-types, and it can be finished by specific optical analysis disc.Different tests may require different dishes, and the coded message on the dish provides and the relevant information of concrete test for PC 158.

Figure 19 is a top plan view of removing the CD drive assembly of outer cover, is used to show pan arrest 168, main shaft 170, and carriage assembly 172, head assemblies 174 and ribbon-style cable 178, it transmits the signal that commutes head assemblies.Carriage assembly 172 provides along the rectilinear motion of track 173 for head assemblies 174.Head assemblies 174 comprises the lens subassembly 176 that is used for incident light and reflected light focusing adjustment.

Figure 20 is the bottom perspective view of CD drive assembly shown in Figure 19, and it shows the practical layout of chipset, the ribbon-style cable 178 when ribbon-style cable connector 179 breaks away from head assemblies 174 (Figure 19) on relevant circuit and the circuit board 177.Can obtain to commute the signal that head assemblies 174 sends, or, connect the lead end of banded cable connector 179 to circuit board 177 directly from ribbon-style cable 178, or the specific weld spot on circuit board 177 180.

Figure 21 is the block scheme according to the relation of linking to each other between the prior art CD reader 140 of embodiment of the present invention II (Fig. 9 A) and the buffer amplifier card 152.Comprise to come A in the self-detector 18 according to chipset 30 of the present invention (Fig. 8), B, the tap of C and D output terminal.Figure 21 also illustrates, also can extract F-out from the HF matrix amplifier 18A of CD drive 140, F+, T-, T+, HF-AC coupled signal and HF-DC coupled signal.These are extracted out, and signal can be linked into detecting device 18 and HF matrix amplifier 18A produces the original simulating signal that is untreated.This allows external device (ED) received signal under the condition of not interfering the operation of driven device.This external device (ED) can comprise: remodeling PC 142, audio processing modules 156, outside ADC 150, or external buffer amplifier card 152 and outside ADC 150 shown in Figure 9.As already pointed out, Figure 21 is the embodiment of the present invention II shown in Fig. 9 A.

Figure 22 is the top perspective according to the external buffer amplifier card 152 (Fig. 9 A and 21) of a practical embodiments among A to the D embodiment of the present invention, and it is suitable for from head assemblies 174 (Figure 19) and drives impact damper 151 received signals.The gentle operation signal of washing disk drive off of this circuit arrangement output.Signal from head assemblies 174 enters into buffer amplifier card 152 at the contact pin of connector 155.This signal is through resistor independently, and capacitor and operational amplifier group are exaggerated and cushion, and are directed to output 157 then.The buffer amplifier card 152 of this embodiment provides 9 to 11 output signals, comprising: Quadrant A, Quadrant B, Quadrant C, Quadrant D, detecting device E, detecting device F, lack of balance HF, balanced HF, the AC HF that be coupled, tracking servo response and focus servo respond.

Figure 23 is the skeleton view of the external buffer amplifier card 152 shown in Figure 22 of another embodiment.Input signal from head assemblies enters at connector 155.Output 157 is passed through in the signal output that is directed into the internal drive impact damper 151 (Figure 12) of CD drive, and the signal after handling is conducted through connector 159 arrival external buffer amplifier cards 152 (Figure 12).

Figure 24 is Figure 24 A, the diagrammatic representation that concerns between 24B and the 24C.Figure 24 A, 24B and 24C are the amplifier stage circuit diagrams according to first embodiment impact damper card shown in Figure 22 and 23.

Figure 24 A is the partial circuit synoptic diagram of buffer amplifier.From the simulation HF signal 50 (Fig. 4) of head assemblies 174 (Figure 19) is that

contact pin

1 and 2 from connector 155 (Figure 22 and 23) takes out.The two ends that input signal is transferred to input load resistor and voltage regulation capacitor just going between with equilibrium and negative lead-in wire between ground unrest.Then, positive signal is fed into the operational amplifier that is cushioned by the variable feedback loop.Before the HF1 signal of connector J5 output, amplifying signal is directed into the two ends of output load resistor and voltage regulation capacitor in becoming output 157 (Figure 22).

From the simulation F+ signal of head assemblies 174 is that

contact pin

3 and 4 from connector 155 takes out.The two ends that input signal is transferred to input load resistor and voltage regulation capacitor just going between with equilibrium and negative lead-in wire between ground unrest.Then, positive signal is fed into the operational amplifier of the feedback control loop buffering that is fixed.Before becoming the FC+ signal, amplifying signal is directed into the two ends of output load resistor and voltage regulation capacitor.

From the simulation F-signal of head assemblies 174 is that

contact pin

5 and 6 from connector 155 takes out.The two ends that input signal is transferred to input load resistor and voltage regulation capacitor just going between with equilibrium and negative lead-in wire between ground unrest.Then, positive signal is fed into the operational amplifier of the feedback control loop buffering that is fixed.Before becoming the FC-signal, amplifying signal is directed into the two ends of output load resistor and voltage regulation capacitor.

From the simulation T+ signal of head assemblies 174 is that contact pin 7 and 8 from connector 155 takes out.The two ends that input signal is transferred to input load resistor and voltage regulation capacitor just going between with equilibrium and negative lead-in wire between ground unrest.Then, positive signal is fed into the operational amplifier of the feedback control loop buffering that is fixed.Before becoming the TC+ signal, amplifying signal is directed into the two ends of output load resistor and voltage regulation capacitor.

From the simulation T-signal of head assemblies 174 is that

contact pin

9 and 10 from connector 155 takes out.The two ends that input signal is transferred to input load resistor and voltage regulation capacitor just going between with equilibrium and negative lead-in wire between ground unrest.Then, positive signal is fed into the operational amplifier of the feedback control loop buffering that is fixed.Before becoming the TC-signal, amplifying signal is directed into the two ends of output load resistor and voltage regulation capacitor.

Figure 24 B is another part circuit diagram of buffer amplifier.From the simulation HF-AC signal of head assemblies 174 (Figure 19) is that

contact pin

11 and 12 from connector 155 (Figure 22 and 23) takes out.Input signal transmission is by the input load capacitor, the two ends that arrive input load resistor and voltage regulation capacitor more just going between with equilibrium with negative the lead-in wire between ground unrest.Then, positive signal is fed into the operational amplifier that is cushioned by the variable feedback loop.Before the HF-AC signal of connector J3 output, amplifying signal is directed into the two ends of output load resistor and voltage regulation capacitor in becoming output 157 (Figure 22).

From the simulation HF-A signal of head assemblies 174 is that

contact pin

19 and 14 from connector 155 takes out.The two ends that input signal is transferred to input load capacitor and voltage regulation capacitor just going between with equilibrium and negative lead-in wire between ground unrest.Then, positive signal is fed into the operational amplifier of the feedback control loop buffering that is fixed.Before the HF-A signal of connector J8 output, amplifying signal is directed into the two ends of output load resistor and voltage regulation capacitor in becoming output 157.This signal also output lead before voltage regulation capacitor is drawn out of, and presents A to the D circuit of HF-A signal to middle HF2 (DC) output of connector J7 (Figure 24 C).

From the simulation HF-B signal of head assemblies 174 is that

contact pin

17 and 16 from connector 155 takes out.The two ends that input signal is transferred to input load capacitor and voltage regulation capacitor just going between with equilibrium and negative lead-in wire between ground unrest.Then, positive signal is fed into the operational amplifier of the feedback control loop buffering that is fixed.Before the HF-B signal of connector J9 output, amplifying signal is directed into the two ends of output load resistor and voltage regulation capacitor in becoming output 157 (Figure 22).This signal also output lead before voltage regulation capacitor is drawn out of, and presents A to the D circuit of HF-B signal to middle HF2 (DC) output of connector J7 (seeing Figure 24 C).

From the simulation HF-C signal of head assemblies 174 is that

contact pin

15 and 18 from connector 155 takes out.The two ends that input signal is transferred to input load capacitor and voltage regulation capacitor just going between with equilibrium and negative lead-in wire between ground unrest.Then, positive signal is fed into the operational amplifier of the feedback control loop buffering that is fixed.Before the HF-C signal of connector J10 output, amplifying signal is directed into the two ends of output load resistor and voltage regulation capacitor in becoming output 157 (Figure 22).This signal also output lead before voltage regulation capacitor is drawn out of, and presents A to the D circuit of HF-C signal to middle HF2 (DC) output of connector J7 (seeing Figure 24 C).

From the simulation HF-D signal of head assemblies 174 is that

contact pin

13 and 20 from connector 155 takes out.The two ends that input signal is transferred to input load capacitor and voltage regulation capacitor just going between with equilibrium and negative lead-in wire between ground unrest.Then, positive signal is fed into the operational amplifier of the feedback control loop buffering that is fixed.Before the HF-D signal of connector J11 output, amplifying signal is directed into the two ends of output load resistor and voltage regulation capacitor in becoming output 157 (Figure 22).This signal also output lead before voltage regulation capacitor is drawn out of, and presents A to the D circuit of HF-D signal to middle HF2 (DC) output of connector J7 (seeing Figure 24 C).

Figure 24 C is another part circuit diagram of buffer amplifier.Be conducted through independently input electricity group device and make up from the FC+ of Figure 24 A and FC-signal.The signal of combination is fed into the negative input end of operational amplifier, and variable positive voltage is fed into positive input terminal.Be fixed feedback control loop buffering of amplifying signal, and be conducted through the negative input end that variohm enters into second operational amplifier.By the second fixing feedback control loop buffering, and before the FOCUS of connector J2 exports during becoming output 157 (Figure 22), be directed into the two ends of output resistor and voltage regulation capacitor from the amplifying signal of second operational amplifier.

Be conducted through independently input electricity group device and make up from the TC+ of Figure 24 A and TC-signal.The signal of combination is fed into the negative input end of operational amplifier, and variable positive voltage is fed into positive input terminal.Be fixed feedback control loop buffering of amplifying signal, and be conducted through the negative input end that variohm enters into second operational amplifier.By the second fixing feedback control loop buffering, and before the TRACKING of connector J6 exports during becoming output 157 (Figure 22), be directed into the two ends of output resistor and voltage regulation capacitor from the amplifying signal of second operational amplifier.

From the tap HF-A of Figure 24 B, HF-B, HF-C and HF-D are guided respectively by importing electricity group device and making up.The signal of combination is fed into the negative input end of operational amplifier, and variable positive voltage is fed into positive input terminal.Be fixed feedback control loop buffering of amplifying signal, and be conducted through the negative input end that variohm enters into second operational amplifier.By the second fixing feedback control loop buffering, and before the HF2 (DC) of connector J7 exports during becoming output 157 (Figure 22), be directed into the two ends of output resistor and voltage regulation capacitor from the amplifying signal of second operational amplifier.

Change CD drive-software

According to other principles of the present invention, can able to programmely reconfigure chipset 30 (Fig. 8), therefore, it is unwanted that physics changes CD drive.A kind of method is by programming DSP 32 (Fig. 8) it to be moved as A/D converter, rather than as demodulator/decoder.In this configuration, dsp chip replaces outside ADC 150, and directly provides digitizing HF signal to host data bus.By analyzing the digitizing HF signal that forms, can detect the investigation structure.Perhaps, the untreated HF signal of route connects this signal to personal computer (for example, PC 158) by the outlet terminal (Fig. 9 A) of chipset 30 to CD drive 140, with utilize hardware in the personal computer and/or software to finish the A/D conversion, can detect the investigation structure.

Can programmable configuration DSP 32 as A/D converter, and do not need the additional modulation and the error recovery of several different methods.For example, in the program storage 39 (Fig. 8) configuration routine of storage can via controller 38 to reconfigure DSP 32.Perhaps, application program can reconfigure DSP 32 by interface circuit 36 (Fig. 8) as required selectively.When DSP 32 received certain type HF signal, DSP 32 also can be configured to A/D converter by itself.These methods only are illustrative, if desired, can utilize any other suitable software or firmware based on relocation method or path.

Figure 25 is the functional-block diagram that the digital signal processing circuit programmable configuration is become analog to digital converter according to the embodiment principle of embodiment of the present invention III shown in Figure 9.Figure 25 illustrates that according to the present invention the processing resource in the DSP 32 can reconfigure to form the certain methods of suitable A/D converter.For example, in a kind of possible arrangement, A/D assembly 42 can break away from path 45, is directly connected to output interface 48 by path 43.In this case, the complete bypass functionality piece 44 of digitizing HF signal and 46, and be transferred to output interface 48.In another kind is arranged, transmit on path 45 from the digitized signal of A/D functional block 42, and

functional block

44 and 46 and not processed are passed through in transmission.In certain embodiments, may need temporarily to make

functional block

44 and 46 and power supply disconnects or place low power operational mode to reduce power consumption (for example, in battery-driven disk drive) to them.Though several possible A/D converter arrangements of above description if desired, can utilize any other adequate resources arrangement in the DSP 32.

If can the unwanted function of bypass by programming, then do not need to change existing hardware, change with the driving firmware but need to do some.

Figure 26 is some flow chart of steps that detect the investigation element according to relating among the DSP embodiment of the present invention shown in Figure 25.As shown in figure 26, when needs enter detecting pattern (step 100), the signal processing system that internal drive is divided is configured to as analog to digital converter operation (step 101).This can comprise the one or more chips that reconfigure chipset 30 (for example, DSP 32) able to programme, and it is the routine that adopts remote application or utilize storage in the local program storer 39 (Fig. 6).This conversion operations does not just need actual changes disk drive circuit, and its permission the present invention utilizes the interior configurable processing resource of chipset 30.

In step 102, utilize objective lens unit 10 (Fig. 1), from the CD 130 (Figure 12) that comprises investigation feature 136 (Figure 12), obtain a plurality of analog data signals.Secondly, in step 103, the combine analog data-signal is to produce summation (HF) signal 50 (Fig. 4) and tracking error (TE) signal 52 (Fig. 4).In step 104, these two signals are offered signal processing system.In step 105, from TE signal 52, extract the desired essential information of operational light disk drive (for example, following the tracks of focusing, and speed control).Meanwhile, signal processing system converts HF signal 50 to digitized signal and in step 105, digitized signal is offered output interface 36 (Fig. 8).Subsequently, the digitizing summing signal is used to describe investigation feature or the structure 136 that exists on the CD 130.In case the scanning process of finishing can the boot disk driver 140 withdraws from (step 106) from detecting pattern.At this moment, be configured to the original configuration that the segment chip group (for example, DSP 32) of A/D converter can be got back to it in the past, and can recover normal CD, CD-R, or DVD operation.

In a variation scheme of triggered mark 166 (Figure 13) and trigger circuit 167 devices, can encode and recording disk assembly 130 on monomark.Some digital binary auto-correlation sign indicating number (that is the sequence of binary digit) can be used for the monomark of encoding.For example, known Barker code is a series of position (number of variation is up to 13), it peak value equal bits number (in registration or when relevant) and side lobe (do not register or when relevant) equal that precipitous autocorrelation function was arranged at 1 o'clock.See Barker, R.H., " Group Synchronization of Binary DigitalSystems ", it is published in Jackon, W. Bian Ji Communications Theory, Academic Press, New York, 1953, pp.273-287 is incorporated in that this is for reference.

In an object lesson, known 13 Barker code is 1111100110101.When the digital bit stream that comprises these 13 long codes was relevant with these 13 long codes of search receiver as a reference, registration generation completely was relevant with 13 identical bits.Yet if bit stream slides forward or backward up to 12, the relevant side lobe of peak value only has 1.

Other autocorrelation function is known, and its low side lobe is outside correlation, and high side lobe is within correlation.See also Lindner, J., " Binary Sequences Up ToLength 40 With Best Possible Autocorrelation Function ", and ElectronLetters, vol.11, p.507, October 1975.For example, it is 25 known code that two length are arranged, and wherein being expressed as octadic one is 163402511.Octal digit is to change to 7 from 0, its representative ordering binary number be 000,001,010,011,100,101,110 and 111.The peak-peak side lobe of this yard is 2, and its correlation peak is 25.It is 40 known code that 114 length are arranged, and one of them is expressed as octadic is 14727057244044.The peak-peak side lobe of this yard is 3, and its correlation peak is 40.

High correlation when long auto-correlation sign indicating number also can detect and register accurate yard with the low correlation with random order that receives in the sequence with in bit stream is described.The bit stream that writes down on the CD assembly can be configured to comprise the auto-correlation sign indicating number in this data bit stream.So the signal processor of analyzing bit stream is relevant the auto-correlation sign indicating number of seeking in bit stream and the incoming bit stream.When associative processor ran into auto-correlation sign indicating number in the bit stream, related function is the very high spike of appearance when normal correlation is relevant with the stream of random bits data.This provides a monomark, and its identical meaning is that triggered mark 166 provides a monomark.

Refer again to Figure 12, CD assembly 130 is divided into sector that investigation feature or signal element 136 are arranged in the target area 135 and the sector 133 that comprises the operation information that is used for the operational light disc system.In this variation scheme, operation information preferably comprises data, and one of them a little data comprises the auto-correlation sign indicating number.These data be stored in according to known method on the sector of CD assembly 130 (for example, CD ROM, DVD, or the like).In the data stream of CD assembly, detect and during registration auto-correlation sign indicating number, two timers (or circuit of equal value) are set in PC 158.Comprising that the sector of investigating feature begins is to stop marking with first timer, is to mark with the second timer termination and comprise that the sector of investigate feature finishes.The duration of these two timers preferably includes in the data of storage on CD assembly 130, and therefore, common optical disk system can be used for having the dissimilar biological dish of different size sector, storage investigation feature in the biology dish.

Conventional CD reader generally allows the user to play CD, and the ability that provides control to read parameter to the user is very little.As a rule, the user of commercialization CD and DVD player does not need this ability.Utilize software after sale, can finish the improvement of these firmware bases usually.In other words, programming operation can provide maybe on dish and can pass through the Internet download.

It is the technology shown in Figure 87 that the another kind of conventional CD reader improves.Channels bits generator 1000 is used to produce known good data position, then, they is added to the data bit of actual reception from the CD that comprises biological position is read, pick-up 1002 as shown in the figure.The position send to block decoder 1004 with value, can obtain the piece error message.

Be applicable to that added improvement of the present invention includes, but not limited to following one or more abilities:

1. swinging chute is play and is linked into swinging chute at random, and does not need the startup that begins from dish.This allows driver to enter into LBA (or address of some other patterns) and plays forward from this.

2. inquire about the laser detection value, it allows to read the laser power value that the laser power monitor detecting device detects in the optical pickup units.

3. inquiry and setting laser power are read/the broadcast value, and it allows user's monitoring and sets the power command value of giving laser instrument.

4. inquiry automatic gain control (AGC) is to obtain the AGC value.Ride gain has consistent amplitude with the signal of guaranteeing to detect.So amount of gain is the reverse indication of signal intensity.Therefore, this signal can be used for detecting and measuring.

5. inquire about the automatic gain control value of following the tracks of.

6. the C1 at monitoring port place and/or the activity of P1 demoder are with monitoring type of error and acquisition error count.This is useful, because mistake can be to detect the useful information of investigation feature locations.Gap in the conventional driver detection coded data is as mistake.

7. the C2 at monitoring port place and/or the activity of PO demoder.See above the 6th.

8. initialization and follow the tracks of operating characteristics on the analysis disc be independent of codimg logic.This is meant the ability of control laser position and console panel speed, it and data independence.This function allows the user to send order with the rotation of maintenance driver motor, and does not have its focusing, follows the tracks of and synchronous operation function.

9. with specific speed and laser read-out power initialization driver.Driver has fixing toggle speed and laser power usually.It is to be set by the user and to change that this variation allows these values.Yet in typical disk driver system, CD gets started rotation to obtain focus point, obtains synchronizing information and finds catalogue listing.If do not find information, then CD drive opens and closes.In some cases, when dish is inserted into CD drive, may need its not rotary CD.For example, when liquid sample adds on the dish, need prevent to coil automatic rotation.This variation also relates to the variation of following the 15th setting.

10. comprising All Ranges on the dish of introducing and drawing, stream transmission main channel and sub-channel data, it can make the more parts of dish that data are arranged.

Be worth port or secondary port 11. push original EFM (8-14 modulation), it allows the user to see 14 bit data before converting 8 place values to.This function can make the user more clearly accurately know on the dish what is arranged.Be similar to above the 10th, this variation allows more zone on the utilization dish.

12. the DC coupled signal that pushes buffering is to outside port, for example, and TE, FE and HF signal.Ability that provides these signals to be for further processing to outside port is provided for this, and these signals generally are (the seeing Fig. 4 and Fig. 8) that uses for inner.

13. the value of collecting from power calibration district (PCA) and program storage district (PMA) when decoding and inquiry initialization.This can collect more information.

14. the broadcast of time-out dish is also opened tracking servo with monitoring open-loop tracking signal, it allows the excentricity of user's monitor disk.CD has some excentricities usually, so when CD rotated, tracking signal had periodic form.The excentricity of dish derives from the imperfect processing of dish.Therefore, tracking signal is the excentricity reflection that produces cyclical signal, and cyclical signal is the reflection of excentricity.Change as if the reflectivity in the zone, for example, have the investigation feature, then tracking signal reflects the variation of this reflectivity.

15. set virtual initialization logic.As above the 9th pointed, when CD is put in the disk drive, it begin usually the rotation.An Elementary Function is to find catalogue listing.Therefore, this change to allow user to provide catalogue listing to disc drive controller, can cheat disk drive effectively and expect that it reads catalogue listing from dish.

16. the interactive following function of turn-offing.

17. control and monitoring are with or without the focus bias of following function.Focus bias changes the size of laser facula, thereby changes the energy that incides on the CD.In some cases, may need to provide heat to optimize condition determination to CD or panel.So the ability of control focus bias can make the user control heat distribution.

18. each layer on the exchange DVD dish.

19. monitor value changes at switching port.

Read CD or CD-RW 20. utilize DVD laser.DVD laser is on shorter wavelength, and it can be used for imaging and fluoroscopic examination.Have the device of reading CD or DVD ability and be equipped with two laser instruments usually, every kind of pattern has a laser instrument.

21. utilize DVD laser with any frequency-tracking swinging chute (1.2mm).

22. the value of differential phase detection (DPD) signal of monitoring buffering.The DPD signal is the DVD signal that is used to follow the tracks of, and therefore, it is corresponding to the ability of monitoring tracking signal discussed above.

23. utilize the near field of light assembly to detect the structure of destroying the laser diode diffraction limit.

24. utilize servo control mechanism to detect defective or structure.When defective or structure when being relevant with the surface, servo control mechanism pushes signal according to a kind of mode.When defective or structure are on the surface when following, servo control mechanism is worked according to another kind of mode.

25. utilize the phase delay in the DVD optical assembly to detect existing with structure on the detection CD.

26. utilize the DPD focus servo circuit to have the structure that produces digital biotic component with detection.

27. set up the second surface making optical disk system that seam tracking through weaving torch utilizes.The DVDRAM driver is set up the DVD premaster as basic unit and utilize swinging chute to follow the tracks of.Utilize ablation technology to form the hole.

28. utilize the secondary focusing position and second laser that tracker and biological position detection plane and system are isolated.This design is subjected to the influence of biological position ball distribution character.

Utilize the EEPROM existing CD-ROM driver of upgrading

Some softwares of describing in the prosthomere improve and can finish to the EEPROM (Electrically Erasable Programmable Read Only Memo) (EEPROM) in the existing CD-ROM driver by software upgrading.When utilizing user's CD reader,, can upgrade or dispose the operation of reader by the EEPROM in the cds circuit that upwards loads program.This program replaces or the locational information of interpolation EEPROM.Write this program is used to measure biological substance or biological position with optimization driver operation.This program also changes the information characteristics with drive communication.This program also changes during measurement or detecting operation or the information of collecting from driver after the operation.

On the CD of this program can be included in bootable (EL Torito technical stipulation) form.Byte is placed on known position or sector (CD or DVD sector), and it tells information on qualified operating system (guidable BIOS or firmware) the utilization dish as operating system.This ability for other I/O systems on most of BIOS or the market (that is, and Adaptec, Award, Phoenix, or the like) be very common.In fact, the byte of identification can tell calculation element to reinstall the operating system that is included on the dish.Then, operating system is included in loading program on the dish.

Load program and be written to the form of manufacturer's technical stipulation.Load program and be written to the content of application technology regulation.Utilize the communication protocol of port, move to the EEPROM in the drive circuit loading program.These agreements comprise ATAPI 1﹠amp; The SCSI device form (iso standard) of 2 (Small Form FactorCommittee 80201-80901) and

SCSI

1,2 and 3 industrial technology regulation described in byte command.The physics summary of communicating by letter in SCSI and the ATAPI device is actually different.Allowing some manufacturers to utilize does not have the private byte communication format of covering in this standard.The present invention can utilize these special technology regulations.The chipset of drive manufacturers utilization also has some agreements, and these agreements are written as program form and contents of program.

Collect from driver by port (SCSI or ATAPI) in the information that run duration is collected from driver, and make an explanation by the software program that is referred to as driver.Driver can partly be included in operating system (for example, Microsoft Windows ^TM) or the specific drivers of part drive manufacturers in.Driver can also partly be included in the chipset of manufacturer, and this chipset is used for the circuit operation communication port.Driver can also be replaced or modification by specific program, and is encased in operating system by loading program on the dish.

From the data of adjusting the driver collection is to make an explanation from the physical port collection and by driver.The data of collecting also with ATAPI and technical stipulation form that the SCSI device is communicated by letter in narrate.These data can also be collected by the professional format of packing on the driver.This professional format can comprise the chipset communication protocol of adjustment, the port communication agreement of adjustment, or adjustment or off-gauge driver.

The exclusive data zone relates to Reed-Soloman demoder collection data in service from CD reader.This comprises the C1 and the C2 activity of CIRC in the dvd playback unit (the Reed Soloman of intersection) sign indicating number, interior and the outer parity checking activity of RSPC in the DVD broadcast unit (Reed-Soloman product) sign indicating number, or be included in the disk format CCRC/CRC (cyclic redundancy check (CRC) code) information of any or sector strucre.These data offer the port or the driver of packet format, and it is relevant with the bio-measurement of the present invention's design.

The DVD technology

Utilize the DVD technology to make shorter wavelength in the laser diode and be included in the operation boundary that the huge increase of density and operation information provides in the dish form very big increase has been arranged.Comprise pearl, cell, aurosol, the biological position or the signal element of carbon, or other micro-mark and the indicator relevant with CD can be positioned at DVD and coil the 0th layer or the 1st layer of assembly.The CD design relevant with this respect of the present invention is at the common unsettled U.S. Patent application sequence No.10/006 that transfers the possession of, have in 620 more fully and describe, its title is " Multiple Data LayerOptical Discs for Detecting Analytes ", application in Dec 10 calendar year 2001 is incorporated in it that this is for reference.

The 0th layer of the DVD dish is made into the second surface dish.This dish can be made the information that has on the internal diameter seldom, therefore, it not can with the detection generation interference of light of biological position on the 1st layer of external diameter.Can utilize the 0th layer as adaptive light clapboard, it is placed on the biological position of processing and uses on afterwards the 1st layer.The 1st layer is made into the second surface dish.Usually, utilize special glue that it is pasted on the 0th layer, this glue and moulded plastic layer have similar refractive properties (for example, between 1.54 and 1.58).This can keep optical efficiency and in reflection or return very little loss of signal is arranged in the light path.The specific thickness of this layer is the 40-60 micron.Transmission property preferred design outer or the 0th layer becomes like this, the significant loss that it can compensating signal causes because of small air interface.

Dvd system is designed to provide automatic signal gain and surface reflectivity to be low to moderate the recovering information of 30% (only being defined in dual layer format).In this method, may need to tilt automatically control.In using, this technology utilizes a plurality of laser instruments.The 0th layer can become operation layer, and it comprises the information that is used to provide systems operational requirement.The second layer is on the 1st layer, is used for the detection of biological position, signal element or investigation feature.The 1st layer can or can not comprise the hole, platform, or groove.In a specific embodiment, biological position, signal element or investigation feature are added to the DVD-R with subregion synchronous clock, DVD-RW, or DVD-RAM uses or the swinging chute in band hole is used." mixing " dish in the employing dvd system is as the basis of this biological position detection technique.The meaning of word " mixing " is to use multiple density.For example, the 0th layer is DVD density, and the 1st layer is CD density.

This thought is to set up the mixed type dish shown in Figure 88 A and the 88B.Figure 88 A is the side view of mixed type dish, and Figure 88 B is the top view of mixed type dish.Make the CD part of 8cm, and it is pasted the center of single laser DVD-RAM or DVD-RW dish.The CD part is represented the 1st layer of DVD dish basically, and comprises the information relevant with finishing biologicall test, for example, and drive circuit, the program of dish speed etc.Shown in Figure 88 A, the 1st layer 1008 (8cm CD player) is on the 0th layer 1006.At the 0th layer is substrate layer 1016 below (1006).At the end of CD part, the instruction driver jumps to the 0th layer 1006, and the exterior portion of reading dish.Structure to be measured is placed in the recess 1014 below the top cover 1012.Several recesses are distributed in the different sectors of dish, and they are the exterior portion in the 0th layer (1006) shown in Figure 88 B.In another embodiment, can utilize kapillary to replace recess.Because the space between layer L0 and the layer L1 is the magnitude of 40 μ m, can place big biological structure.

In this embodiment, top cover comprises biologicall test, and needs to replace after each mensuration.The dish remainder can reuse in the mensuration afterwards, as long as it be the cleaning to prevent cross pollution.

A kind of exploitation the in the DVD technology can make the DVD driver according to being included in the temporary transient reprogramming of the information of reading on the dish.This just provides a kind of means to DVD drive circuit reprogramming, can finish the existence of detection of biological position and describe the desired any special processing in any this detection of biological position.For example, biological position can be deposited as the special graph on the dish.This dish also comprises and is used for the procedure code whether test pattern exists, thereby can the detection of biological position.This just relates to the code and the DVD circuit of correspondingly programming of exploitation such as RLL (2,10) sign indicating number.

A difference in the DVD of detection of biological position technology implementation scheme relates to utilizes swinging signal.But between the swinging signal that utilizes in CD register system and the dvd system material difference is arranged.But in the CD register system, the present invention utilizes swinging signal can compare to keep the tracking signal of speed control with reference pulse to provide.The secondary swinging signal is absorbing.It utilizes two-phase label coding technology and several other information is provided.The two-phase labeling method is a Ditital modulation method.This digital waveform is added on the swinging signal, but and the two-phase demoder is included in the CD record cell with decoded information.The numerical information of encoding in the swinging signal comprises: logical place, power control information and specific information.The manufacturing technology of two-phase mark and swinging signal is very unique.Make CD and can write down the very special-purpose manufacturing premaster technology of premaster requirement.The system that makes premaster utilizes two laser of making premaster.A laser is used to make swinging chute, and secondary laser is modulated into sets up the two-phase label information in swinging signal.

But in the DVD register system, the element of use is to be similar to very much element described above.The technology of making premaster also is a similar techniques roughly.The locking frequency of using in this technical specification is 140kHz or 7.143 microseconds.Under the rotating speed of 3.49 meter per seconds, the physics of a swing figure approximates 25 microns.Most of biotic components that this still attempts to measure greater than the present invention.

But our exactissima diligentia is the design of DVD rewriteable standard.This standard utilization tracking and simultaneous techniques, the platform that it is referred to as to swing and groove form.The swinging signal frequency increases to 160kHz or 6.25 microseconds.Under the rotating speed of 3.49 meter per seconds, the physics that can measure is about 21.8 microns.

But in the DVD rewriteable standard, but the address information that the two-phase marker graphic provides in the CD record standard no longer digital modulation to swinging signal.Embossing formula hole structure is placed on the ad-hoc location around the dish.These " subregions " comprise address information and some timing informations.This record scheme is referred to as ZCLV or subregion constant linear velocity technology.Zone between these " subregions " comprises groove and swinging signal.Unique feature of utilizing in the DVD-RAM technology is that platform/groove exchange is followed the tracks of.In this technology, we exchange to the groove that next time rotates from the platform that once rotates.This is very uncommon, but high-caliber control is provided.The embossing formula hole structure that platform has control to use, and groove comprises the zone of phase-change recording.

In the present invention, the increase of the biological bit pattern density of capabilities limits of decoding two-phase label information.These subregions provide the accurate zone of detection of biological bit pattern.This technology is utilized the space on the CD, but compromise proposal is very big gain in setting accuracy.

Improve resolution

The power monitoring signal provides and is similar to spectrophotometric response in CD or the DVD register system.By the logical message on the dish or by software, power control signal or monitor signal that can the controlling recording laser diode.When being focused into irradiating light beam and moving in the CD district, it can also comprise the analysis of monitor signal.

The birefringence in another examples measure CD district.This relates to the remodeling player with second additional optical distance, does not also have this player on the current market.This light path relates to and utilizes prism to replace rotatory polarization.

In the employed art of mathematics, can use biological position in the decoding processor of player, the optimization of signal element or investigation feature detects shows to replace look-up table (being used to finish modulation or the information movement from the data bit to the channels bits).The mathematics of adjusting to stroke (RLL 2,10) in the Reed Soloman Code And Decode scheme can be optimized to does the detection of biological position.These adjustment are provided for detecting or the information of the biological position of statistical appraisal.Utilize the standard software of C1 on the CD interface decoder or C2 mistake to detect and to obtain this information.Can also utilize the standard software of PI on the DVD interface decoder or PO mistake to detect and also can obtain this information.PI/PO data from the DVD demoder can be used for describing biological position, the size of signal element and investigation feature.In a specific embodiment, EFM or ESM figure that look-up table produces are replaced by simple 8 bit pattern, and it describes the stroke of institute's research project.By being included in the program in the canned data on the CD, may be completed to the variation of decode system in the player.This information is encased in " fast " EPROM or similar techniques.

Another embodiment utilizes solid immersion lens (SIL) in the technology of detection of biological position.The SIL player improves operation resolution greatly.

But another embodiment utilization is optimized to the microstructural CD recording played machine on the optical disc surface of reading.Optics is adjusted this player to detect the structure of air interface on the optical disc surface.But but CD is designed to utilize the CD register system to provide quantitative measurment microstructural workbench.

The photo bio dish

Figure 27 is the profile perspective of primary structure unit in the embodiment photo bio dish 410.Figure 27 is the example of operable echo area photo bio dish 410 among the present invention (below be referred to as " reflection-type dish ").These primary structure unit comprise: top layer part 416, adhesive 418 and substrate 420.Top layer part 416 comprises access road 422 and exit passageway 424.Top layer part 416 can utilize polycarbonate to make, and preferably from the bottom of a fluoroscopic observation shown in Figure 27 coating reflecting surface 446 (seeing Figure 29).In this preferred embodiment, triggered mark 166 (Figure 12) is included on the surface of reflection horizon 442 (seeing Figure 29).Triggered mark 166 can comprise transparent window in all three layers of biology dish, the opacity, or reflection or half reflection district, last face code (for example sends to processor, ADC 150 shown in Figure 12) information of data, the operating function of this processor and inquiry or incident beam 137 (Figure 12) interacts.Second unit among Figure 27 is bonding coat or channel layer member 418, and it has stream 428 or forms the U-shaped passage therein.Stream 428 is to make by punching press or cutting film, removes the shape shown in plastic foil and the formation.Each stream 428 can comprise flow passage 430 and backward channel 432.Some streams 428 shown in Figure 27 comprise mixing chamber 434.Two dissimilar mixing chambers 434 draw among the figure.First mixing chamber is symmetrical mixing chamber 436, and it is symmetrically formed with respect to flow passage 430.Second mixing chamber is biasing mixing chamber 438.A side of flow passage 430 shown in biasing mixing chamber 438 is formed on.The 3rd unit is the substrate 420 that comprises target area or trapping region 135 shown in Figure 27.Substrate 420 is preferably made by polycarbonate, and the reflection horizon 442 (seeing Figure 29) that is deposited on its top is arranged.Target area 135 is to make by the reflection horizon 442 of shape shown in removing or any required form.Perhaps, can utilize mask technique to make target area 135, mask technique is included in the zone that adds the district 135 that covers over the object before the reflection horizon 442.Can utilize metal to make reflection horizon 442, for example, aluminium or gold.

Figure 28 is the top plan view of photo bio dish 410 shown in Figure 27, and reflection horizon 442 is on transparent top layer part 416, can manifest stream 428, target area 135, access road 422, exit passageway 424 and the triggered mark 166 that is positioned at dish.

Secondly, with reference to Figure 29, Figure 29 represents the enlarged perspective according to the echo area type photo bio dish 410 of one embodiment of the invention.This skeleton view comprises a part that is cut in each layer, is used to show each main layer, substrate, the fragmentary cross-sectional view of coating or film.Figure 29 represents to apply the substrate 420 in reflection horizon 442.Active coating 444 is added on the reflection horizon 442.In a preferred embodiment, active coating 444 can utilize polystyrene to make.Perhaps, can utilize polycarbonate, gold, activation glass, remodeling glass, or remodeling polystyrene, for example, polystyrene-co-maleic anhydride.In addition, can utilize hydrogel.Perhaps, shown in this embodiment, bonding coat 418 is added on the active coating 444.The expose portion of bonding coat 418 is showed cutting or the punching press U-shaped shape of setting up stream 428.Main structural sheet is a top layer part 416 in this biological dish echo area embodiment.Top layer part 416 is included in the reflecting surface 446 on its bottom.Reflecting surface 446 can be made with metal, for example, and aluminium or gold.At the common unsettled U.S. Patent application of transferring the possession of 10/035, in 836, the application that openly has the type disc shown in Figure 29 of genetic test, its title is " Surface Assembly for Immobilizing DNA CaptureProbes and Bead-Based Assay Including Optical Bio-Discs andMethods Relating Thereto ", Dec 21 calendar year 2001 application is incorporated in it that this is for reference.

Referring now to Figure 30, Figure 30 represents the primary structure unit section skeleton view according to transmission-type photo bio dish 410 of the present invention.Similarly, the primary structure unit of transmission-type photo bio dish 410 comprises: top layer part 416, bonding coat 418 and substrate 420.Top layer part 416 comprises access road 422 and exit passageway 424.Top layer part 416 can be made of layer of polycarbonate.Optional triggered mark 166 can be included on the surface of thin semi-reflective layer 443, as shown in figure 33.Triggered mark 166 can comprise transparent window in all three layers of biology dish, opacity, or reflection or half reflection district, last face code (for example sends to processor, ADC 150 shown in Figure 12) information, this processor interacts with the operating function of inquiry light beam 137 (Figure 12).

Unit second shown in Figure 30 is bonding coat or the channel layer member 418 that stream 428 is arranged or wherein form the U-shaped passage.Stream 428 is to make by punching press or cutting film, removes the shape shown in plastic foil and the formation.Each stream 428 can comprise flow passage 430 and backward channel 432.Some streams 428 shown in Figure 30 comprise mixing chamber 434.Two dissimilar mixing chambers 434 draw among the figure.First mixing chamber is symmetrical mixing chamber 436, and it is symmetrically formed with respect to flow passage 430.Second mixing chamber is biasing mixing chamber 438.A side of flow passage 430 shown in biasing mixing chamber 438 is formed on.

The 3rd unit is the substrate 420 that comprises target area or trapping region 135 shown in Figure 30.Substrate 420 is preferably made by polycarbonate, and the semi-reflective layer 443 (shown in Figure 34) that is deposited on its top thin is arranged.Semi-reflective layer 443 thickness relevant with 410 substrates 420 of dish shown in Figure 31 and 34 are than Figure 27, and reflection horizon 442 thin thickness on 410 substrates 420 of reflection-type dish shown in 28 and 29 are a lot.Thin semi-reflective layer 443 allows part to inquire the structural sheet of light beam 137 transmission by transmission-type dish shown in Figure 12.Utilize metal can make thin semi-reflective layer 443, for example, aluminium or gold.

Figure 31 is the enlarged perspective of substrate 420 and semi-reflective layer 443 in the transmission-type embodiment photo bio dish 410 shown in Figure 30.In a preferred embodiment, Figure 30, thin semi-reflective layer 443 thickness of transmission-type dish shown in 33 and 34 are about 100-300 , and are no more than 400 .This thin semi-reflective layer 443 allows a part of incident beams or inquiry light beam 137 (Figure 12) to penetrate by semi-reflective layer 443 to be detected by top detector 160 (Figure 12), and some light are reflected and return along incident path.As shown below, table 2 provides the reflection characteristic of golden film and the relation between transmissison characteristic and the film thickness.Under the situation of thickness greater than 800 , golden membranous layer reflects fully.Light transmission is about 400 by the threshold densities of golden film.

Table 2

The reflectivity and the transmissivity (absolute value) of gold film
				Thickness ()	Thickness (nm)	Reflectivity	Transmissivity
????0	????0	????0.0505	????0.9495	Thickness ()	Thickness (nm)	Reflectivity	Transmissivity
????0	????0	????0.0505	????0.9495	????50	????5	????0.1683	????0.7709
????100	????10	????0.3981	????0.5169	????50	????5	????0.1683	????0.7709
????100	????10	????0.3981	????0.5169	????150	????15	????0.5873	????0.3264
????200	????20	????0.7142	????0.2057	????150	????15	????0.5873	????0.3264
????200	????20	????0.7142	????0.2057	????250	????25	????0.7959	????0.1314
????300	????30	????0.8488	????0.0851	????250	????25	????0.7959	????0.1314
????300	????30	????0.8488	????0.0851	????350	????35	????0.8836	????0.0557
????400	????40	????0.9067	????0.0368	????350	????35	????0.8836	????0.0557
????400	????40	????0.9067	????0.0368	????450	????45	????0.9222	????0.0244
????500	????50	????0.9328	????0.0163	????450	????45	????0.9222	????0.0244
????500	????50	????0.9328	????0.0163	????550	????55	????0.9399	????0.0109
????600	????60	????0.9448	????0.0073	????550	????55	????0.9399	????0.0109
????600	????60	????0.9448	????0.0073	????650	????65	????0.9482	????0.0049
????700	????70	????0.9505	????0.0033	????650	????65	????0.9482	????0.0049
????700	????70	????0.9505	????0.0033	????750	????75	????0.9520	????0.0022
????800	????80	????0.9531	????0.0015	????750	????75	????0.9520	????0.0022

Except the table 2, Figure 32 provides the inverse ratio curve figure based on reflectivity properties and transmission property in the thin semi-reflective layer 443 of golden thickness.The reflectivity of curve shown in Figure 32 and transmittance values are absolute values.

Figure 33 is the top plan view of the transmission-type photo bio dish 410 shown in Figure 30 and 31, has represented the fluid passage 428 that is positioned at dish, access road 422, exit passageway 424, triggered mark 166, and target area 135.

Figure 34 is photo bio dish 410 enlarged perspectives according to transmission-type dish embodiment of the present invention.Cut in the dish 410 each layer part and can show each main layer, substrate, the fragmentary cross-sectional view of coating or film.Figure 34 represents the transmission-type disk-form, and it has transparent top layer part 416, the semi-reflective layer 443 and the triggered mark 166 that approach on the substrate 420.Triggered mark 166 comprises the opaque material that top layer is partly upward placed.Perhaps, triggered mark 166 can be made of transparent non-reflection windows or any mark of etching on the thin reflection horizon 443 of dish, and this mark absorbs or do not reflect the signal from detection trigger device 160 (Figure 12).Figure 34 also shows the target area 135 of formation, and it is in shape or any required shape shown in the formation of appointed area.Mark on the target area 135 can be formed on the thin semi-reflective layer 443, on the substrate 420, or the bottom of substrate 420 (below dish).Perhaps, can utilize mask technique to form target area 135, this technology comprises whole thin semi-reflective layer 443 outside the district 135 that covers over the object.In this embodiment, utilize screen printing ink to be added on the thin semi-reflective layer 443, can set up target area 135.Active coating 444 is added on the thin semi-reflective layer 443.In a preferred embodiment, active coating 444 is thick-layers of 2% polystyrene.Perhaps, can utilize polycarbonate, gold, activation glass, remodeling glass, or remodeling polystyrene, for example, polystyrene-co-maleic anhydride.In addition, can utilize hydrogel.Perhaps, shown in this embodiment, bonding coat or channel layer 418 are added on the active coating 444.The expose portion of bonding coat 418 is showed the U-shaped shape of the cutting or the punching press of setting up stream 428.Last primary structure layer is transparent non-reflective top layer part 416 among the biological dish of this transmission-type 410 embodiment, and it comprises access road 422 and exit passageway 424.

Refer again to Figure 12, under the situation of the biological dish of reflection-type shown in Figure 29, Returning beam 139 is top layer part 416 reflectings surface 446 (the seeing Figure 35) reflections from photo bio dish 410.In this reflection-type embodiment of photo bio dish 410, utilize floor detection device shown in Figure 1 18 to detect and analyze Returning beam to determine existing of signal element or reagent.The Returning beam of this reflection alternately or simultaneously carries operation information and biological position, the characteristic information of signal element or investigation feature.On the other hand, under the situation of the biological dish of transmission-type, top detector 160 detects and analyzes transmitted light beam 162 to determine existing of signal reagent.In transmission-type embodiment, can utilize photoelectric detector as top detector 160.Hardware trigger mechanism can be used for biological dish of reflection-type (Figure 29) and the biological dish of transmission-type (Figure 34).

Figure 35 is the fragmentary cross-sectional view according to photo bio dish 410 among the reflection-type dish embodiment of the present invention.Figure 35 illustrates substrate 420 and reflection horizon 442.As mentioned above, reflection horizon 442 can be by such as aluminium, and gold copper-base alloy or other suitable reflecting materials are made.In this embodiment, the upper surface of substrate 420 is smooth.Figure 35 also shows the active coating 444 that is added on the reflection horizon 442.The formation of target area 135 is the zones that need by shelter before removing reflection horizon 442 partly on the desired location or adding reflection horizon 442.As shown in Figure 35, bonding coat 418 is added on the active coating 444.Figure 35 also shows top layer part 416 and relevant reflecting surface 446 thereof.Therefore, when top layer part 416 is added to when comprising on the required bonding coat 418 that cuts shape, just form flow passage 430 therein.Incident beam 137 is to be drawn towards substrate 420 from coiling 410 belows at first, focuses on the point with reflection horizon 442 vicinities then.Because this focusing occurs in the target area 135 that does not have reflection horizon 442 parts, incident beam 137 continues along the path by active coating 444 and enters flow passage 430.Then, incident beam 137 continues upward through flow passage 430, and finally incides reflecting surface 446.At this moment, incident beam 137 returns along incident path, and forms Returning beam 139.

Figure 36 is the fragmentary cross-sectional view according to biological dish 410 among the transmission-type dish embodiment of the present invention.Figure 36 explanation has the transmission-type disk-form of semi-reflective layer 443 thin on transparent top-layer part 416 and the substrate 420.Figure 36 also shows the active coating 444 that is added on the thin semi-reflective layer 443.In a preferred embodiment, the transmission-type dish has metal to make thin semi-reflective layer 443, for example, aluminium or gold, its thickness is about 100-300 , and is no more than 400 .This thin semi-reflective layer 443 allows from the part incident beam of light source 19 (Fig. 1) or inquires the dish that light beam 137 penetrates and upwards pass through to be detected by top detector (Figure 12), and some only are reflected along the reverse direction with the incident beam same paths.In this device, Returning beam or folded light beam 139 are from semi-reflective layer 443 reflections.Therefore, in this manner, Returning beam 139 does not enter flow passage 430.Reflected light or Returning beam 139 can be used for following the tracks of the incident beam 137 on the pre-recorded information magnetic track, and the pre-recorded information magnetic track is formed in the semi-reflective layer 443 or on the semi-reflective layer 443, will describes in more detail below in conjunction with Figure 37 and 38.In dish embodiment shown in Figure 36, can be with or without definite target area 135.By directly on thin reflection horizon 443 or substrate 420, forming mark, can set up target area 135.Utilize silk screen printing or any suitable method, can set up these marks.Do not adopting physical token to determine among another embodiment of target area, utilizing flow passage 430 as the target area that limits, the inspection of the feature of investigating in this target area.

Figure 37 gets perpendicular to the biological sectional view that coils 410 magnetic tracks among the reflection-type dish embodiment according to the present invention.This sectional view also is the vertical of flow passage 430 radiuses in the dish of edge.Figure 37 comprises substrate 420 and reflection horizon 442.In this embodiment, substrate 420 comprises the series of grooves 118 shown in Fig. 3.Groove 118 is spiral fashion forms, extends to outward flange near the center of dish.Make such groove 118, make inquiry light beam 137 go up spiral slot 118 and follow the tracks of along dish.Rising or bossing 110 (Fig. 3) are separated adjacent groove 170 in the spiral slot.In this embodiment, the reflection horizon 442 that is added on the groove 118 is conformals.Figure 37 also shows the active coating 444 that is added on the reflection horizon 442.The formation of target area 135 is the zones that need by shelter before removing reflection horizon 442 partly on the desired location or adding reflection horizon 442.As shown in figure 37, bonding coat 418 is added on the active coating 444.Figure 37 also shows top layer part 416 and relevant reflecting surface 446 thereof.Therefore, when top layer part 416 is added to when comprising on the required bonding coat 418 that cuts shape, just form flow passage 430 therein.

Figure 38 gets perpendicular to the biological sectional view that coils 410 magnetic tracks among the transmission-type dish embodiment according to the present invention.This sectional view also is to go up the vertical of flow passage 430 radiuses along dish.Figure 38 shows substrate 420 and thin reflection horizon 443.Thin semi-reflective layer 443 allows from the part incident beam of light source 19 (Fig. 1 and 12) or inquires that light beam 137 penetrates and pass through the dish that top detector 160 detects, and some light are reflected into the form of Returning beam 139.Determine that thin semi-reflective layer 443 thickness are the desired minimal reflection light quantities of tracking power that keep it according to CD reader.In this embodiment, substrate 420 comprises series of grooves 118, as what discussed among Figure 37.Groove 118 among this embodiment preferably also is the spiral fashion form, extends to outward flange near the center of dish.Make such groove 118, inquiry light beam 137 can be followed the tracks of along spiral slot.Figure 38 also shows the active coating 444 that is added on the thin semi-reflective layer 443, and bonding coat 418 is on active coating 444.Figure 38 also shows does not have the top layer of reflecting surface 446 part 416.Therefore,, just form flow passage 430 therein when top layer part 416 is added to when comprising on the required bonding coat 418 that cuts shape, and allow part incident beam 137 basically reflectingly transmission pass through.

Figure 39 is the view that is similar to Figure 35, and it shows the whole thickness of reflection-type dish and initial refractive properties thereof.Figure 40 is the view that is similar to Figure 36, and it shows the whole thickness of transmission-type dish and initial refractive properties thereof.The groove 118 that do not draw among Figure 39 and 40 is because sectional view is along groove 118 cuttings.Figure 39 and 40 shows among these embodiment the narrow flow passage 430 perpendicular to groove 118.Figure 37,38,39 and 40 show the whole thickness of reflection-type dish and transmission-type dish.In these accompanying drawings, incident beam is initial to interact with the substrate 420 with refractive properties, and refractive properties changes the path of incident beam, thereby makes incident beam 137 focus on reflection horizon 442 or thin semi-reflective layer 443.

Optimize the photo bio dish

The optimization of dish is used to detect microstructural surface to provide in the optical disk system.Set up the CD in the light correction player discussed above.For example, this dish manufactures CD recordable disc " reversed image " or direct picture.At the common U.S. Patent application sequence No.10/005 that transfers the possession of, disclose this CD and relevant manufacture method in 313, its title is " Optical Discs forMeasuring Analytes ", applies for Dec 7 calendar year 2001, it is incorporated in this is for reference.A kind of such dish is a dish 130 shown in Figure 41, and it is as " backswing " dish.The dish 130 of this embodiment comprises: reverse dish substrate 132, its forms top layer or layer farthest.This dish also comprises: biological position is arranged, and the groove 118 of signal element or investigation feature 136, they preferably are arranged in groove.Utilize non-integral lid 138 as adjacent top with respect to incident beam 137.

In CD-R (CD-R) system, the polycarbonate of laser focusing by 1.2mm (or similarly refractive material), and focus in the bathtub construction of filling dyestuff, this dyestuff has absorbent properties.The CD that is used for this detection system is " reversed image " of making the CD-R dish.Reversed image allows CD reader to contact with the first surface or the air interface of succeeding vat.Air interface allows microstructure is placed on the surface that can read.

Utilize electroforming or " nickel pressing mold " production method to make CD, this method is referred to as " Matrixing ".The nickel image is set up on surface according to CD " master ", can be placed on electroforming process (or similar procedure) to it, and can set up reversed image." master " is often referred to " father " part." reversed image part " is often referred to " mother " part.In many CD manufacture processes, " mother " part is used to make another reversed image, and it is referred to as " son " part." father " part has identical direct picture with " son " part." mother " part has reversed image.In this application, but the premaster that this process allows normal CD record-keeping system dish process to make.Utilizing the correct direct picture of this dish is feasible and possible to set up " father " part technically.Reversed image partly is to be made by nickel, and is used in the CD mold pressing machine to set up the parts of plastics of representing inverse image.

Because its geometric configuration, it partly is difficult utilizing reversed image in the CD manufacture process.The edge of direct picture part is open, and the edge of reversed image part is closed.Mould must be designed for the CD mold process.This mould is equipped with additional exit passageway, allows polycarbonate or other mold pressing refractive again material correctly to move on the surface of nickel part.The exit passageway design of adding on the mould can make required CD make according to the mode that is similar to CD dish or CD-R dish.

Required CD is designed to best succeeding vat planform, and the succeeding vat structure originates in the interior diameter of dish and ends at the overall diameter of dish.Groove depth is optimized to the tracking amplitude (push-pull signal) that can provide very strong.The groove depth of making is in close proximity to 1/8 of laser wavelength lambda, and this laser incides on the air interface of CD.The groove depth of making also can be these values odd-multiple (for example, 1/8 λ, 3/8 λ, 5/8 λ, or the like).The width of CD groove is optimized to layout or the size detection of being convenient to structure, and this structure is placed on the optical disc surface.Therefore, in one embodiment, adopt 1/8 wavelength to recommend tracing deviation.The gradient of groove structure is optimized to the tracking signal response of best groove focal position and optimization.The optimization of finishing succeeding vat inner platform district is to reduce the signal cross-talk and the detection of optimizing structure.

The air plane of incidence of CD manufactures with respect to the microstructure of placing on the panel surface optical contrast ratio.If the microstructure absorbing light is then placed reflection-type material (for example, gold) on the air plane of incidence.If the microstructure reflected light is then placed non-reflection-type material on the air plane of incidence.But CD manufactures best mechanical property is arranged in CD recording played machine.

The air plane of incidence of reversed image dish provides light discussed above to proofread and correct the required operation requirement of player.Adjusting lens permission reversed image dish has the performance of the CD-R dish that is similar to non-record.By the software interface of user CD-R driver, can obtain this test pattern.Player can place test pattern, and follows the tracks of " swing " groove and pass through the block of information.Utilize user CD-R swinging chute form, can the wobble detection rooved face on the position to 1/75 second of composition.Positional information in the user CD-R form is included in master " swing " signal, and can arrive user's driver by software interface safely.The microstructure that is placed on the reversed image panel surface can not require the operation of swinging chute system to exert an influence, unless they reach very high concentration (data collecting point).In fact, swinging chute allows microstructure to be placed on the optical disc surface, and can not require to produce adverse influence to the operation of CD-R system.

Quaternary summing signal by monitoring CD-R objective lens unit can detect the architectural characteristic of placing on the reversed image panel surface now.Be added to focus servo, in the electric signal of tracking servo and power Control and Monitor System, also can detecting these microstructural electroresponses.In being added to the electric signal of tracking servo circuit, can detect along be parallel to the reversed image panel surface radially with the bare headed kinetic characteristic of tangential plane.In being added to the electric signal of focus servo circuit, can detect along bare headed kinetic characteristic on the vertical plane perpendicular to the reversed image panel surface.Can be the information cross-reference of these signals and quaternary summing signal and power monitoring signal collection.In the mathematics form relevant, can analyze the information of each response in these responses with the microstructure dimensional characteristic that detects on the reversed image panel surface.Can utilize differential mathematics or vector analysis to extract each microstructural characteristic.

With in the focal position that is retained in laser facula, can design that structure has maximum detection resolution on the reversed image panel surface by layout and dimensional requirement.Microstructure should be designed to the reversed image panel surface maximum optical contrast ratio is arranged, and strong input and low electric CNR can be provided.The microstructure that is added on the reversed image panel surface should be sufficiently little of in the focussing plane that is retained in laser facula.Because dimensional characteristic is better than the focal position, the fraction signal is to detect in quaternary summation detecting device, and most of signal is to detect in other operation servo control mechanism.

Dish can be designed to hold the dedicated slot of microstructure size characteristic.Microstructure can be designed to be placed in the groove of reversed image dish.This can be used for the detection by quantitative characteristic of further optimization system.Microstructure is made into " spherical ".When these balls were placed on the reversed image panel surface, they produced the very electric signal of characteristic in servo control mechanism, and produce operation signal in driver.Platform area (zone between groove) at dish is set up smooth circular surfaces, and it allows ball to drop into the interior clip position of groove, thereby can easily use electrical signal detection.

As mentioned above, the diameter less than well width can be made in indicator ball or biological position.This permission indicator ball enters into to be convenient in the groove detect, as shown in figure 41.The indicator ball can also be made with compressible material, and its diameter is slightly greater than the width of groove.In one embodiment, suitable flat board is used to press these balls, and they are driven in the groove.

We have described some embodiment, in not departing from claims scope of following statement, obviously can do various changes to it.For example, more than and following used term for reference only, rather than can adjust anything but.

Investigation feature (investigational features)

According to the structure of the present invention's research, feature, characteristic and attribute can comprise: biology, chemistry, or organic sample, test sample book, such as the insect of part or the investigation object of organic material, and similar test object or target sample.This structure, feature and attribute can also comprise: specific chemical reaction and product and/or the secondary product that forms thus, for example, various different colorimetric estimations any.Under the situation of photo bio dish, being added to the material that carries out research and analysis on the dish can comprise: biological particle suspension and organic material, for example, blood, urine, saliva, amniotic fluid, Skin Cell, celiolymph, serum, synovia, sperm, sub-thread and distrand DNA, liquor pleurae is selected from organ to the cell of organizing pericardial fluid, ight soil, peritoneal fluid, and calculus.Under the situation of some this materials, can utilize indicator to detect.The indicator that uses during the present invention describes includes, but not limited to plastic microsphere or latex, the pearl that polystyrene is made, or the aurosol particle of biomolecule coating is arranged, they have affinity to given material, for example, the biotin molecule in the DNA thigh.Suitable coating compounds comprises streptavidin or neutral protein streptavidin.According to the method, the non-detectable too wisp of reading optical beam still can be detected by relevant indicator in the driver.

The dvd playback machine can be used for detecting feature and the character of surface on the CD focal plane.Microstructure, cell, therefore indicator, or " biological position " can add on the focal plane of CD assembly, can detect them the electric signal that produces when the laser of optical disc surface reflection or transmission is collected by objective lens unit and/or top detector.Microstructure and CD workbench are designed to be convenient to accurate detection, and the operation of system is required that minimum influence is arranged.

According on the method surface measurements described below or tiny organism structure and/or spherical structure in the focal plane.These structures on volume greater than 1/2 wavelength that detects light (inciding structural light).

When these microstructures or biological position were present on the surface of CD focal plane, this structure appeared on the surface of platform area.Signal element, biological position, or the investigation feature also may reside on the plane, and this plane is not in next-door neighbour's focousing field, but very approaching with it.Under this situation, these structures enough approach the focousing field that reflector laser can detect.Investigation feature such as biological position also may reside on a plurality of planes, and these planes are what to separate with the laser that satisfies architectural characteristic operation requirement.Biological position and other indicator also can be inserted in groove or the hole, and cause that in reflected light enough interference of light put the detection signal of letter with generation.Biological position also may reside in logical partition or makes in the zone of CD tissue.These zones can be the sizes of platform magnitude or hole magnitude, also can be in the extreme greatly (for example, DVD-RAM).

Detect the investigation feature

The investigation feature, for example, indicator or haemocyte produce signal level or the variable density relevant with signal, and this signal is to produce by the coded message of reading on the dish.The common transfer and unsettled U.S. Patent application No.09/421,870, its title is " Tracking Optical Discswith Concurrently Readable Nonoperational Features ", application on October 26th, 1999, investigation or " not operation " structure of wherein mentioning micron-scale can be configured on the optical disc surface according to several different methods.As mentioned above, Figure 41 describes a suitable embodiment who finishes this configuration.As shown in figure 41, light beam 137 incides on the dish assembly 130 from the below.Dish 130 comprises: dish substrate 132 and reflection horizon 134, investigation structure or feature 136 are configured on the reflection horizon 134.Figure 41 swinging chute 118 of compacting and coating reflection horizon 134 in the substrate 132 that also draws, the top cover 138 of the non-integral of also drawing.Can detect according to CD reader of the present invention, measure and describe investigation structure 136.Utilize (or the non-while) detection simultaneously of single light picker to comprise the dish operating structure of tracking characteristics, the dish operating structure is different from the investigation structure.

Secondly, with reference to Figure 42, it is similar to Fig. 5 discussed above, Figure 42 three hot spots that draw, and they are to produce on typical case's three light beam light devices by the CD assembly that incides hole 60.Dot the

laser beam spot

62,64 and 66 on the optical disc surface.These light beams can focus on the identical faces of dish, for example, and hole 60, or on any other outside surface and inside surface of dish.These light beams also can focus on the different layers of dish, and " layer " is any part of graduated dial, the thickness that this dish is limited, and as at above-mentioned U.S. Patent application No.10/006, disclosed multilayer disc in 620.

In three light beam light disc systems, the detecting device A shown in Fig. 4, B, C and D are configured to detect the light from hot spot 62 reflections, as shown in figure 42.In addition, detecting device E and F are configured to independently respectively from

hot spot

64 and 66 light that reflect.As mentioned above, this configuration can make from the light of hot spot 62 reflections focusing and synchronizing information are provided, and provides trace information from the light of

hot spot

64 and 66 reflections.

Figure 43 represents to be configured in the investigation feature 68 on the typical CD assembly surface.In this device, hot spot 62 can be used for detecting to be followed the tracks of, and focus on and synchronous operating structure (for example, the hole), and hot spot 64 can be used to detect one or more investigation features 68 simultaneously.Perhaps, hot spot 64 can be used for detecting investigation feature 68, and it depends on the size and the position of investigating feature 68.

In addition, if investigation feature 68 is sufficiently big, then

hot spot

64 and 66 can be used in combination (though needing not to be simultaneously) to detect investigation feature 68.Should be known in that the graphics combine of each hot spot can be used for detecting the size and the position of investigation feature 68.And, come self-detector A, B, the figure of C and D can make up with the figure that comes self-detector E and F, is used for determining the size and the position of investigation feature.Therefore, single objective lens unit can the detecting operation structure and the different light paths of investigation feature.Should be known in the detection that the present invention relates to operating characteristics and investigation feature disclosed herein, but it is not limited to the CD assembly of hole and platform form.On the contrary, the present invention can be used for any other form, form as discussed above.

Figure 44 represent that the reading optical beam of driver runs into that dish is gone up or dish in the investigation feature time data-signal (or density) the relative displacement curve.Data-signal can be the HF signal, tracking error signal, and focus error signal, or the one or more signals in various other unlike signals, for example, those signals described above.

In Figure 45 and 46, the variation of " operation " feature, for example, and groove, hole or platform produce signal level, signal jitter, or the variation of error rate.In Figure 45, it is the sectional view in hole, and the hole produces from the signal A of detecting device in the disc reader and the variation of B, produces a fluctuating extraordinary when addition.In Figure 46, it is the planimetric map in hole, and transversal displacement produces the clean displacement of tracking error signal (TE signal).

When incident beam interacts with dish, produce the increase of reflectivity or reduce.Utilize automatic gain control (" AGC ") that middle corresponding variation is set, this variation is to export in the driver port, can monitor this reflectance varies.Therefore, according to the present invention, when the reading optical beam of driver or " inquiry " light beam run into the investigation feature, detect the variation of back light.

The structure of CD can be that the hole and the surface of platform are arranged, and the surface of continuous swinging chute is arranged, and comprises the surface of phase correlation hologram, the surface that has hole/groove to make up, or without any the surface of structure.As mentioned above, can on many different focal planes, measure indicator, biological position, or honeycomb.Utilize a plurality of laser instruments, a plurality of objective lens units and a plurality of optical maser wavelength also can detect and describe biological position.

Indicator, biological position and honeycomb can be on the first type surface of holographic disk.Openly relate to the more detailed content of hologram in this application in the U.S. Patent Application Serial Number 10/005,313.The information of collecting from hologram is used for operating characteristics.As required, biological bit position can near or away from operation planar.Can adjust refractor (polycarbonate, polymethyl or glass) to optimize the required optical property of detection laser.

Perhaps, the investigation feature can comprise the chemical reaction that occurs in the flow passage, for example, the flow passage 430 shown in Figure 27, it be formed on the dish shown in Figure 47 or dish in.In this embodiment, the reflectivity on the CD, operating characteristics or conoscope image are the influences that is subjected to chemogenic deposit or reaction.Utilize low-down error rate or known errors rate as data " mask " manufacturing dish.Datagraphic on the dish is designed to produce the actual mistake that strengthens investigation feature or specific dimensions scope combination results of logical OR.

For example, can in making the stamper logic, adjust cross distance to strengthen burst error to concrete feature, the response that size or density distributes.Datagraphic is written to dish to be gone up to produce to concrete feature the response that size or density distributes.These can include, but not limited to following content:

1. non-time-out;

2. voice signal (to the response of digital silence coding);

3. special-purpose error recovery figure or distribution (for example, E11, E21, E31, train of impulses, E12, E22, E32, E42);

4. uncorrectable error;

5.ECC/EDC counting;

6. interior or outer bad parity;

7. the crc error in the swinging signal demoder;

8. sector mistake (75/sec); Or

9. piece mistake (7350/sec).

In an exemplary embodiments, flow passage is placed in the dish.This dish can have up to 99 magnetic tracks, and groove is arranged on the magnetic track, hole, or CD, CD-R, DVD, DVD-RAM, DVD-R, the combination of DVD-RW operating characteristics.The deposition of concrete chemical reaction or " not operation feature " (ball, metallicity, or the like) can strengthen or remove the material from metallic reflector or operation planar or focal plane.Hot spot or subregion are placed on the different band or magnetic track of dish.Logic in each band is determined the position of objective lens unit.Logic can also be determined " software servo response ".Distribute or effect from low-density, hot spot is placed in each band or the magnetic track with the density of increase or the radial location of increase.On DVD-RAM dish or similar dish, hot spot is placed in the subregion (subregion CLF system).Reaction produces the physical change of " operating characteristics ", and mistake or wrong on-the-spot is brought out in each position on dish.Can assess numeral, simulation, optics, the response of machinery and logic is to describe effect.

In this embodiment, there is the error rate of increase at each scene, and attempts relevant with Fault Distribution: E11, and E21, E31, train of impulses, E12, E22, E32, E42, uncorrectable, expendable, ECC/EDC, or BLER.In a preferred embodiment, this process is to distribute from low-density, and moves to higher Density Distribution, until relevant in discovery between analyte concentration and the Fault Distribution.Preferably be made into and strengthen relevant dish.For example, change interlace operation with intensifier pulse string and C2 response.

With the surface of dish, operation planar, or the reaction of focal plane can be set up and increase or reduced analog signal level or density.This reaction produces the variation of reflectivity or signal density, or the reducing or increases of reflection horizon, or the shape and/or the size of phase place/interference feature (cheating or platform).

As general suggestion, before certain threshold thickness, the reflectivity of metal level is the function of layer thickness, discusses in conjunction with Figure 32 as above.For various metals, as the nickel among Figure 48, aluminium, silver, or gold are equal to or greater than at metal thickness under the situation of threshold thickness, reflectivity be constant and about 80% with near 100% between basic maintenance constant.This reflectivity also depends on metal purity and surface appearance.As with the relevant General Principle of some concrete feature of the present invention, work as metal film, for example, reflection horizon 443 (shown in Figure 34), with the dish flow passage in comprise fluid interaction the time, can change thickness of metal film and surface appearance.

In Figure 49, occur in the fluctuating that chemical reaction in the flow passage 430 of dish shown in Figure 47 causes monitored signal, for example, HF or TE signal.When the inquiry light beam 137 of disk drive crosses by occurring in the dish or during the chemical reaction on the dish, the envelope of signal fluctuation increases with the reaction time or reduces.

According to another aspect of the present invention, the hole on the dish, mark or groove can be made by the chemical interaction material.By the variation of signal response is provided, the degradation level of material can be determined some mensuration characteristics.Chemistry or measure material and can interact with the reflection horizon, and, can reduce or increase transmission, reflection, the light of refraction or absorption according to the reflectance varies of reflection horizon shown in Figure 47 or metal level 443.For example, operating structure can be made by the nitrocellulose cellulosic material.Chemical interaction can change the shape and/or the thickness of operating structure, and therefore, it reduces signal response.In addition, the pH value of solution can make metal deposit to the subregion surface of dish, and produces the increase of local reflectance.In another embodiment, local reaction can cause from reflecting surface and remove metal material.Remove metal material and cause contrast point in the signal.Response can be that simulating signal characteristic or error rate distribute.In addition, subregion can be designed into the dish that is used for distinguishing concentration and reaction.In these embodiments, material is design like this, reduces particular concentration or reaction level.

Figure 50 represents these features of the present invention on the whole.Dish among Figure 50 comprises: subregion A, and B and C, for example, these subregions are formed on the DVD-RAM dish with subregion constant linear velocity (ZCLV).Subregion C comprises the reaction according to this feature of the present invention, has wherein removed the reflection horizon, and in subregion A and B, this metal reaction that removes has not taken place.Also show the HF of formation or the signal trajectory of TE signal.These this signal trajectories explanation has the detectable signal of trajectory generation on the reflecting material subregion, and scanning does not produce sort signal on the subregion of reflecting material not having.

Referring now to Figure 51, Figure 51 shows the cross sectional side view of photo bio dish 200, and biological dish 200 comprises the pearl indicator 210 that utilizes in conjunction with the present invention.Biological dish 200 comprises: substrate 202, metallic diaphragm 204, bonding coat or channel layer 206 and covering disk 208.Substrate 202 comprises hole or groove or other devices, can be according to known method coded message in the above.Substrate 202 covers metallic diaphragm 204 usually on the zone of coded message.Yet, biological dish 200 and known information disk (for example, music, DVD, or the like) difference, comprise investigation structure (being the pearl indicator in the case) on the part dish of biological dish.Chemosphere 214 (for example, antibody) is deposited on the required target area of dish.Each pearl indicator 210 applies the surface of similar or identical chemosphere 212 in addition.Pearl indicator 210 is small baton rounds (or ball of other materials), the interactional chemical reagent of biological chemistry goods in its coating and the solution.

As the object lesson of one aspect of the invention, Figure 52 A provides the diagrammatic representation that is placed on according to the blue pearl of last two the 6.8 μ m of the several magnetic tracks of photo bio dish of the present invention (being labeled as A to H).These blue pearls are placed and stick to and are similar on the dish that coils shown in Figure 51.The track while scan A to H that draws among the figure, wherein several tracks are through the pearl indicator.

Figure 52 B is used to a series of signature tracks from the derivation of pearl indicator shown in Figure 52 A from the AC of CD-ROM driver coupling and buffered HF signal according to the present invention.HF-AC coupled signal from head assemblies 174 (Figure 19) HF matrix amplifier 18A (Figure 21) is directed into buffer amplifier card 152 (Figure 22 and 23).This signal is exaggerated and handles (Figure 24 B), is directed into the out connector J3 of output 157 (Figure 22) then.Send this signal to ADC 150 from buffer amplifier card 152, and handled and monitor 146 (Fig. 9 A and 12) imaging by PC 158.As mentioned above, remodeling PC 142 can replace one or more treating apparatus described herein.Figure 52 B explanation, the scanning on two 6.8 μ m indicator pearls causes detecting the different disturbances of HF signal.

As another object lesson, Figure 53 A provides the diagrammatic representation that is placed on according to two the 6.42 red pearls of μ m on the several magnetic tracks of photo bio dish of the present invention.These red pearls are placed on and are similar on the dish that coils shown in Figure 51.

Figure 53 B is used to a series of signature tracks from red pearl derivation shown in Figure 53 A from the AC of CD-ROM driver coupling and buffered HF signal according to the present invention.HF-AC coupled signal from head assemblies 174 (Figure 19) HF matrix amplifier 18A (Figure 21) is directed into buffer amplifier card 152 (Figure 22 and 23).This signal is exaggerated and handles (Figure 24 B), is directed into the out connector J3 (Figure 22) of output 157 then.Send this signal to ADC 150 from buffer amplifier card 152, and handled and display 146 (Fig. 9 A and 12) imaging by PC 158.As mentioned above, remodeling PC 142 can replace one or more treating apparatus described herein.Figure 53 B explanation, the scanning on two 6.42 μ m indicator pearls causes detecting the different disturbances of HF signal.

As according to another object lesson of the present invention, Figure 54 A provides the diagrammatic representation that is placed on according to two 6.33 μ m polystyrene beads on the several magnetic tracks of photo bio dish of the present invention.These pearls are placed on and are similar on the dish that coils shown in Figure 51.

Figure 54 B is used to a series of signature tracks and relevant signal disturbance from the derivation of pearl shown in Figure 54 A from the AC of CD-ROM driver coupling and buffered HF signal according to the present invention.HF-AC coupled signal from head assemblies 174 (Figure 19) HF matrix amplifier 18A (Figure 21) is directed into buffer amplifier card 152 (Figure 22 and 23).This signal is exaggerated and handles (Figure 24 B), is directed into the out connector J3 (Figure 22) of output 157 then.Send this signal to ADC 150 from buffer amplifier card 152, and handled and monitor 146 (Fig. 9 A and 12) imaging by PC 158.As mentioned above, remodeling PC 142 can replace one or more treating apparatus described herein.Figure 54 B explanation, the scanning on two 6.33 μ m polystyrene indicator pearls causes detecting the different disturbances of HF signal.

As another example of some feature of the present invention, Figure 55 A provides the diagrammatic representation that is placed on according to one 5.5 μ m glass indicator pearl on the several magnetic tracks of photo bio dish of the present invention.This pearl is placed on and is similar on the dish that coils shown in Figure 51.

Figure 55 B is used to a series of signature tracks from the derivation of pearl shown in Figure 55 A from the AC of CD-ROM driver coupling and buffered HF signal according to the present invention.HF-AC coupled signal from head assemblies 174 (Figure 19) HF matrix amplifier 18A (Figure 21) is directed into buffer amplifier card 152 (Figure 22 and 23).This signal is exaggerated and handles (Figure 24 B), is directed into the out connector J3 of output 157 (Figure 22) then.Send this signal to ADC 150 from buffer amplifier card 152, and handled and monitor 146 (Fig. 9 A and 12) imaging by PC 158.As mentioned above, remodeling PC 142 can replace one or more treating apparatus described herein.Figure 55 B explanation, the scanning on one 5.5 μ m glass indicator pearl causes detecting the disturbance of HF signal.

Another example of the present invention is to provide in Figure 56 A, and Figure 56 A shows the diagrammatic representation that is placed on according to one 4.5 μ m magnetic bead on the several magnetic tracks of photo bio dish of the present invention.This pearl is placed on and is similar on the dish that coils shown in Figure 51.

Figure 56 B is used to a series of signature tracks from the derivation of pearl shown in Figure 56 A from the AC of CD-ROM driver coupling and buffered HF signal according to the present invention.HF-AC coupled signal from head assemblies 174 (Figure 19) HF matrix amplifier 18A (Figure 21) is directed into buffer amplifier card 152 (Figure 22 and 23).This signal is exaggerated and handles (Figure 24 B), is directed into the out connector J3 (Figure 22) of output 157 then.Send this signal to ADC 150 from buffer amplifier card 152, and handled and monitor 146 (Fig. 9 A and 12) imaging by PC 158.As mentioned above, remodeling PC 142 can replace one or more treating apparatus described herein.Figure 56 B explanation, the scanning on one 4.5 μ m magnetic indicator pearl causes detecting the disturbance of HF signal.

Figure 57 A is the diagrammatic representation that is placed on according to two blue pearls of reality 4.0 μ m on the several magnetic tracks of another example photo bio dish of some feature of the present invention.These pearls are placed on and are similar on the dish that coils shown in Figure 51.

Figure 57 B is used to a series of signature tracks and relevant signal disturbance from blue pearl derivation shown in Figure 57 A from the AC of CD-ROM driver coupling and buffered HF signal according to the present invention.HF-AC coupled signal from head assemblies 174 (Figure 19) HF matrix amplifier 18A (Figure 21) is directed into buffer amplifier card 152 (Figure 22 and 23).This signal is exaggerated and handles (Figure 24 B), is directed into the out connector J3 (Figure 22) of output 157 then.Send this signal to ADC 150 from buffer amplifier card 152, and handled and monitor 146 (Fig. 9 A and 12) imaging by PC 158.As mentioned above, remodeling PC 142 can replace one or more treating apparatus described herein.Figure 57 B explanation, the scanning on two 4.0 μ m indicator pearls causes detecting the disturbance of HF signal.

As another example of the present invention, Figure 58 A provides the diagrammatic representation that is placed on according to one 2.986 μ m polystyrene bead on the several magnetic tracks of photo bio dish of the present invention.This pearl is placed on and is similar on the dish that coils shown in Figure 51.

Figure 58 B is used to a series of signature tracks from the derivation of pearl shown in Figure 58 A from the AC of CD-ROM driver coupling and buffered HF signal according to the present invention.HF-AC coupled signal from head assemblies 174 (Figure 19) HF matrix amplifier 18A (Figure 21) is directed into buffer amplifier card 152 (Figure 22 and 23).This signal is exaggerated and handles (Figure 24 B), is directed into the out connector J3 (Figure 22) of output 157 then.Send this signal to ADC 150 from buffer amplifier card 152, and handled and monitor 146 (Fig. 9 A and 12) imaging by PC 158.As mentioned above, remodeling PC 142 can replace one or more treating apparatus described herein.Figure 58 B explanation, the scanning on one 2.986 μ m polystyrene indicator pearl causes detecting the disturbance of HF signal.

As another example of the present invention, Figure 59 A provides the diagrammatic representation that is placed on according to two the 2.9 white pearls of μ m on the several magnetic tracks of photo bio dish of the present invention.These pearls are placed on and are similar on the dish that coils shown in Figure 51.

Figure 59 B is used to a series of signature tracks from the derivation of pearl shown in Figure 59 A from the AC of CD-ROM driver coupling and buffered HF signal according to the present invention.HF-AC coupled signal from head assemblies 174 (Figure 19) HF matrix amplifier 18A (Figure 21) is directed into buffer amplifier card 152 (Figure 22 and 23).This signal is exaggerated and handles (Figure 24 B), is directed into the out connector J3 (Figure 22) of output 157 then.Send this signal to ADC 150 from buffer amplifier card 152, and handled and monitor 146 (Fig. 9 A and 12) imaging by PC 158.As mentioned above, remodeling PC 142 can replace one or more treating apparatus described herein.Figure 59 B explanation, the scanning on two 2.9 μ m indicator pearls causes detecting the unlike signal disturbance of HF signal.

Figure 60 A provides the diagrammatic representation that is placed on according to four 2.8 μ m magnetic beads on the several magnetic tracks of photo bio dish of some another object lesson of feature of the present invention.These pearls are placed on and are similar on the dish that coils shown in Figure 51.

Figure 60 B is used to a series of signature tracks from the derivation of magnetic bead shown in Figure 60 A from the AC of CD-ROM driver coupling and buffered HF signal according to the present invention.HF-AC coupled signal from head assemblies 174 (Figure 19) HF matrix amplifier 18A (Figure 21) is directed into buffer amplifier card 152 (Figure 22 and 23).This signal is exaggerated and handles (Figure 24 B), is directed into the out connector J3 (Figure 22) of output 157 then.Send this signal to ADC 150 from buffer amplifier card 152, and handled and monitor 146 (Fig. 9 A and 12) imaging by PC 158.As mentioned above, remodeling PC 142 can replace one or more treating apparatus described herein.Figure 60 B explanation, the scanning on four 2.8 μ m magnetic beads causes detecting the different disturbances of HF signal.

As another example, Figure 61 A provides and is placed on according to comprising 2.8 μ m magnetic beads on the several magnetic tracks of photo bio dish of the present invention, the blue polystyrene bead of 4.0 μ m and 6.8 μ m, and the pearl mixed graph of different big or small silica beads is represented.These pearls are placed on and are similar on the dish that coils shown in Figure 51.

Figure 61 B is according to the AC coupling and buffered HF signal a series of signature tracks and relevant signal disturbance from Figure 61 A shown in pearl derivation of utilization of the present invention from CD-ROM driver.HF-AC coupled signal from head assemblies 174 (Figure 19) HF matrix amplifier 18A (Figure 21) is directed into buffer amplifier card 152 (Figure 22 and 23).This signal is exaggerated and handles (Figure 24 B), is directed into the out connector J3 (Figure 22) of output 157 then.Send this signal to ADC 150 from buffer amplifier card 152, and handled and monitor 146 (Fig. 9 A and 12) imaging by PC 158.As mentioned above, remodeling PC 142 can replace one or more treating apparatus described herein.Figure 61 B explanation, the scanning on the indicator pearl mixes causes detecting the different disturbances of HF signal.

Figure 62 A provides the diagrammatic representation that is placed on according to two the 2.9 white fluorescence polystyrene beads of μ m on the several magnetic tracks of photo bio dish of the present invention.These pearls are placed on and are similar on the dish that coils shown in Figure 51, but in this example, utilize DC coupled signal rather than above AC coupled signal in conjunction with the discussion of example track shown in Figure 52 B to 61B.

Figure 62 B is used to a series of signature tracks from the derivation of pearl shown in Figure 62 A from the DC of CD-ROM driver coupling and buffered HF signal according to the present invention.HF-DC coupled signal from head assemblies 174 (Figure 19) HF matrix amplifier 18A (Figure 21) is directed into buffer amplifier card 152 (Figure 22 and 23).This signal is exaggerated and handles (Figure 24 B), is directed into the out connector J5 (Figure 22) of output 157 then.Send this signal to ADC 150 from buffer amplifier card 152, and handled and monitor 146 (Fig. 9 A and 12) imaging by PC 158.As mentioned above, remodeling PC 142 can replace one or more treating apparatus described herein.Figure 62 B explanation, the scanning on two 2.9 white fluorescence polystyrene beads of μ m causes detecting the different disturbances of HF-DC signal.

Figure 63 A provides and is placed on according to the diagrammatic representation of two the 2.9 white fluorescence polystyrene beads of μ m shown in Figure 62 A on the several magnetic tracks of photo bio dish of the present invention.These pearls are placed on and are similar on the dish that coils shown in Figure 51.In this example, utilize DC coupling and buffering " A " signal obtaining required signal trajectory, below it is described in a more detailed discussion.

Figure 63 B is used to a series of signature tracks of deriving from pearl shown in Figure 63 A from the DC of CD-ROM driver coupling and buffering " A " signal according to the present invention.HF-A coupled signal (Figure 22) from head assemblies 174 (Figure 19) is directed into buffer amplifier card 152 (Figure 22 and 23).This signal is exaggerated and handles (Figure 24 B and 24C), is directed into the out connector J7 (Figure 22) of output 157 then.Send this signal to ADC 150 from buffer amplifier card 152, and handled and monitor 146 (seeing Fig. 9 A and 12) imaging by PC 158.As mentioned above, remodeling PC 142 can replace one or more treating apparatus described herein.Figure 63 B explanation, the scanning on two 2.9 white fluorescence polystyrene beads of μ m causes detecting the different disturbances of HF-A signal.

Figure 64 A is the identical cross sectional side view of photo bio dish 200 (Figure 51) with 64B, and it shows that in more detail the biochemistry between biological dish and the indicator pearl interacts.

Figure 64 A is illustrated in the chemosphere 214 of configuration on the metallic diaphragm 204 in more detail.Chemosphere 212 also is coated on the indicator pearl 210.In Figure 64 B, the indicator pearl with comprise the biological solution of investigating feature 216 and mix, and inject or be added on the biology dish 200 between substrate 202 and the top cover 208.Pearl indicator 210 lip-deep chemospheres 212 and chemosphere 214 attract and adhere to investigate feature 216.In this manner, if the chemicals of being studied (that is, the investigation feature) is in biological solution, then the chemicals of being studied becomes bonding agent, and pearl indicator 210 is adhered to substrate 202.When biology dish 200 was accelerated rotation, its effect was a hydro-extractor.The pearl indicator that does not have to adhere to is driven into the outward flange of dish, and the pearl indicator that adheres to still is evenly distributed in the disk area of coating chemosphere 204.So, only need to determine whether the pearl indicator has been driven into the outward flange of dish.At the common U.S. Provisional Application No.60/257 that transfers the possession of, 705, its title is " Surface Assembly for Immobilizing DNACapture Probes and Bead-Based Assay Including Optical Bio-Discsand Methods Relating Thereto ", application on Dec 22nd, 2000; No.60/292,110, its title is " Surface Assembly for Immobilizing DNACapture Probes using Pellets as Reporters in Genetic AssaysIncluding Optical Bio-Discs and Methods Relating Thereto ", application on May 18 calendar year 2001; And No.60/302,757, its title is " Clinical Diagnostic OpticalBio-Discs and Related Methods for Selection and Detection ofLymphocytes Including Helper-Inducer/Suppressor-Cytotoxic Cells ", and describe this pearl based assays dish example and using method in the application July 3 calendar year 2001.By means of above example, the present inventor illustrates, utilizes indicator pearl described herein, can easily detect the discernible signal of investigation feature.

Figure 65 is the cross sectional side view of photo bio dish 190, and it comprises that the contiguous red blood cell of placing 199 is as the investigation feature according to 191 inquiries of reading optical beam in the CD drive assembly of the present invention.

Biological dish 190 comprises: substrate 192, metallic diaphragm 194, bonding coat or channel layer 196 and top cover dish 198.Substrate 192 comprises: the hole, groove, or according to known method in this area can coded message other devices.Substrate 192 covers metallic diaphragm 194 usually on the zone of coded message.Yet, biological dish 190 and known information disk (for example, music, DVD, or the like) difference, biological dish comprises investigation feature (being haemocyte 199 in the case) on the dish of part.Remove metallic diaphragm 194 from the zone that is used to investigate structure.Trapping agent 195 (for example, antibody) deposits to the zone of removing metallic diaphragm.Haemocyte 199 can comprise the biochemicals of being studied (for example, the specialty chemicals of blood group A or blood group B), and it has affinity to trapping agent 195.In this manner, if there is the chemicals of being studied in biological sample, then the chemicals of being studied becomes bonding agent, and haemocyte 199 is adhered to substrate 192.When biology dish 190 rotated in CD drive, the centrifugal force of generation was driven to the outward flange of dish to the haemocyte that not have to adhere to, and the haemocyte that adheres to still is distributed in the disk area that applies trapping agent 195.So, the haemocyte that utilizes CD reader to detect and quantize to adhere to.Measuring relevant more detailed content with blood typing on this class dish is disclosed in the unsettled U.S. Patent application sequence of common transfer, No.09/988,850, its title is " Methods and Apparatus for BloodTyping with Optical Bio-Discs ", November 19 calendar year 2001 application is incorporated in it that this is for reference.

As the detection example according to some feature cell indicator of the present invention, Figure 66 A provides to be close on the magnetic track that is placed on photo bio dish 190 shown in Figure 65 A and places the diagrammatic representation that diameter is about 6.0 μ m red blood cells 199.

Figure 66 B is used to a series of signature tracks from the derivation of red blood cell shown in Figure 66 A from the AC of CD-ROM driver coupling and buffered HF signal according to the present invention.HF-AC coupled signal from head assemblies 174 (Figure 19) HF matrix amplifier 18A (Figure 21) is directed into buffer amplifier card 152 (Figure 22 and 23).This signal is exaggerated and handles (Figure 24 B), is directed into the out connector J3 (Figure 22) of output 157 then.Send this signal to ADC 150 from buffer amplifier card 152, and handled and monitor 146 (Fig. 9 A and 12) imaging by PC 158.As mentioned above, remodeling PC 142 can replace one or more treating apparatus described herein.Figure 66 B explanation causes detecting the disturbance of HF-AC coupled signal in contiguous scanning of placing on the red blood cell.

Figure 67 A is placed on the diagrammatic representation that is about 6.0 μ m red blood cells according to contiguous placement diameter on the photo bio disk track of the present invention.In this example, the red blood cell shown in Figure 67 A is placed on the dish of Figure 65 shown type.

Figure 67 B is used to a series of signature tracks from the derivation of red blood cell shown in Figure 67 A from the DC of CD-ROM driver coupling and buffered HF signal according to the present invention.HF-DC coupled signal from head assemblies 174 (Figure 19) HF matrix amplifier 18A (Figure 21) is directed into buffer amplifier card 152 (Figure 22 and 23).This signal is exaggerated and handles (Figure 24 A), is directed into the out connector J5 (Figure 22) of output 157 then.Send this signal to ADC 150 from buffer amplifier card 152, and handled and monitor 146 (Fig. 9 A and 12) imaging by PC 158.As mentioned above, remodeling PC 142 can replace one or more treating apparatus described herein.Figure 67 B explanation, the scanning on the contiguous red blood cell of placing causes detecting the disturbance of HF-DC signal.

Figure 68 is the cross sectional side view that is similar to the photo bio dish 190 that coils shown in Figure 65 according to the present invention, and it comprises away from the investigation feature of the red blood cell of placing 199 as 191 inquiries of reading optical beam in the CD drive assembly.

Biological dish 190 comprises: substrate 192, metallic diaphragm 194, bonding coat or channel layer 196 and top cover dish 198.Substrate 192 generally comprises: hole or groove, or according to known method can coded message other devices, except the zone of placing the investigation feature.Substrate 192 covers metallic diaphragm 194 usually on the zone of coded message, but except the zone of placing the investigation feature.Biological dish 190 and known information disk (for example, music, DVD, or the like) difference, biological dish comprises investigation feature (being haemocyte 199 in the case) on the dish of part.Remove metallic diaphragm 194 from the zone that is used to investigate structure.Trapping agent 195 (for example, antibody) deposits to zone on the top cover dish 198 that removes metallic diaphragm.Haemocyte 199 can comprise the biochemicals of being studied (for example, the specialty chemicals of blood group A or blood group B), and its attracts and adheres to trapping agent 195.In this manner, if there is the chemicals of being studied in biological sample, then the chemicals of being studied becomes bonding agent, and haemocyte 199 is adhered to dish top cover 198.When biology dish 190 rotated in CD drive, the centrifugal force of generation was driven to the outward flange of dish to the haemocyte that not have to adhere to, and the haemocyte that adheres to still is distributed in the disk area that applies trapping agent 195.Utilize above referenced U.S. Patent application sequence No.09/988, the CD reader of describing in 850, the haemocyte that can detect and quantize to adhere to.

Figure 69 A is placed on the photo bio disk track diameter to be about two of 6.0 μ m away from the diagrammatic representations of placing red blood cell in this example according to these features of the present invention.Red blood cell shown in Figure 69 A is placed on the dish of Figure 68 shown type.

Figure 69 B is used to a series of signature tracks from the derivation of red blood cell shown in Figure 69 A from the AC of CD-ROM driver coupling and buffered HF signal according to the present invention.HF-AC coupled signal from head assemblies 174 (Figure 19) HF matrix amplifier 18A (Figure 21) is directed into buffer amplifier card 152 (Figure 22 and 23).This signal is exaggerated and handles (Figure 24 A), is directed into the out connector J3 (Figure 22) of output 157 then.Send this signal to ADC 150 from buffer amplifier card 152, and handled and monitor 146 (Fig. 9 A and 12) imaging by PC 158.As mentioned above, remodeling PC 142 can replace one or more treating apparatus described herein.Figure 69 B explanation is two different disturbances that cause detecting the HF-AC coupled signal away from the scanning on the red blood cell of placing.

Figure 70 A is two diagrammatic representations away from the placement red blood cell shown in Figure 69 A.Red blood cell shown in Figure 70 A is placed on the dish of Figure 68 shown type.

As another example of some feature of the present invention, Figure 70 B provides according to the present invention and be used to a series of signature tracks of deriving from the DC of CD-ROM driver coupling and buffered HF signal from red blood cell shown in Figure 70 A.HF-DC coupled signal from head assemblies 174 (Figure 19) HF matrix amplifier 18A (Figure 21) is directed into buffer amplifier card 152 (Figure 22 and 23).This signal is exaggerated and handles (Figure 24 A), is directed into the out connector J5 (Figure 22) of output 157 then.Send this signal to ADC 150 from buffer amplifier card 152, and handled and monitor 146 (Fig. 9 A and 12) imaging by PC 158.As mentioned above, remodeling PC 142 can replace one or more treating apparatus described herein.Figure 70 B explanation is two different disturbances that cause detecting the HF-DC signal away from the scanning on the red blood cell of placing.

Figure 71 is the skeleton view that removes the optical test dish 220 of top cover according to the present invention, can show to be placed on the wing 222 of checking insect in the passage 224.Optical test dish 220 shown in Figure 71 also comprises triggered mark 166.Triggered mark 166 provides and the identical function that combines the triggered mark 166 that Figure 12 and 13 goes through.

Figure 71 A is an amplification vertical view of pointing out part among Figure 71, and it shows the wing 222 of insect in more detail, checks passage 224 and according to the information stores magnetic track 226 of this embodiment optical test dish 220 of the present invention.Figure 71 A also shows the incident beam focal beam spot 227 of guiding insect wing 222 into.

Figure 72 is the cross sectional side view of getting perpendicular to optical test dish 220 radiuses among Figure 71, and it comprises that insect wing 222 is as being positioned at the investigation feature of checking passage 224.According to the 225 inquiry insect wings 222 of reading optical beam in the CD drive assembly of the present invention.

Figure 73 A is the diagrammatic representation of the horizontal section of insect wing 222 among Figure 71, and insect wing 222 is placed on according to the inspection passage 224 on the magnetic track 226 in the optical test dish 220 of the present invention.

Figure 73 B is used to the single signature track from the derivation of insect wing transversal section shown in Figure 73 A from the AC of CD-ROM driver coupling and buffered HF signal according to the present invention.HF-AC coupled signal from head assemblies 174 (Figure 19) HF matrix amplifier 18A (Figure 21) is directed into buffer amplifier card 152 (Figure 22 and 23).This signal is exaggerated and handles (Figure 24 A), is directed into the out connector J3 (Figure 22) of output 157 then.Send this signal to ADC 150 from buffer amplifier card 152, and handled and monitor 146 (Fig. 9 A and 12) imaging by PC 158.As mentioned above, remodeling PC 142 can replace one or more treating apparatus described herein.Figure 73 B explanation is in the disturbance that causes detecting the HF signal such as the scanning on the investigation feature of insect wing 222.

Figure 74 A is the diagrammatic representation that is similar to the horizontal section of insect wing shown in Figure 71 222, and insect wing 222 is placed on according to the inspection passage 224 on the magnetic track 226 in the optical test dish 220 of the present invention.

Figure 74 B is used to a series of four the signature tracks in succession from the derivation of insect wing transversal section shown in Figure 74 A from the AC of CD-ROM driver coupling and buffered HF signal according to the present invention.HF-AC coupled signal from head assemblies 174 (Figure 19) HF matrix amplifier 18A (Figure 21) is directed into buffer amplifier card 152 (Figure 22 and 23).This signal is exaggerated and handles (Figure 24 A), is directed into the out connector J3 (Figure 22) of output 157 then.Send this signal to ADC 150 from buffer amplifier card 152, and handled and monitor 146 (seeing Fig. 9 A and 12) imaging by PC 158.As mentioned above, remodeling PC 142 can replace one or more treating apparatus described herein.Figure 74 B explanation is in the different disturbances that cause detecting the HF signal such as the scanning on the investigation feature of insect wing 222.

Figure 75 A is the diagrammatic representation that is similar to the horizontal section of insect wing shown in Figure 71 222, and insect wing 222 is placed on according to the inspection passage 224 on the magnetic track 226 in the optical test dish 220 of the present invention.

Figure 75 B is used to the track of signature in succession from a series of intermediate densities of the derivation of insect wing transversal section shown in Figure 75 A from the AC of CD-ROM driver coupling and buffered HF signal according to the present invention.HF-AC coupled signal from head assemblies 174 (Figure 19) HF matrix amplifier 18A (Figure 21) is directed into buffer amplifier card 152 (Figure 22 and 23).This signal is exaggerated and handles (Figure 24 A), is directed into the out connector J3 (Figure 22) of output 157 then.Send this signal to ADC 150 from buffer amplifier card 152, and handled and monitor 146 (Fig. 9 A and 12) imaging by PC 158.As mentioned above, remodeling PC 142 can replace one or more treating apparatus described herein.Figure 75 B explanation, the intermediate density that scans on the investigation feature such as insect wing 222 track in succession cause detecting the different disturbances of HF-AC signal.

Figure 76 A is the diagrammatic representation that is similar to the horizontal section of insect wing shown in Figure 71 222, and insect wing 222 is placed on according to the inspection passage 224 on the magnetic track 226 in the optical test dish 220 of the present invention.

Figure 76 B is used to the track of signature in succession from a series of higher densities of the derivation of insect wing transversal section shown in Figure 76 A from the AC of CD-ROM driver coupling and buffered HF signal according to the present invention.HF-AC coupled signal from head assemblies 174 (Figure 19) HF matrix amplifier 18A (Figure 21) is directed into buffer amplifier card 152 (Figure 22 and 23).This signal is exaggerated and handles (Figure 24 A), is directed into the out connector J3 (Figure 22) of output 157 then.Send this signal to ADC 150 from buffer amplifier card 152, and handled and monitor 146 (seeing Fig. 9 A and 12) imaging by PC 158.As mentioned above, remodeling PC 142 can replace one or more treating apparatus described herein.Figure 76 B explanation, the higher density that scans on the investigation feature such as insect wing 222 track in succession cause detecting different disturbances with imaging HF signal.

Figure 77 A, 77B and 77C utilize AC coupling and buffered HF signal respectively, DC coupling and buffering " A " signal, or the diagrammatic representation of insect wing shown in Figure 71 that DC is coupled and the buffered HF signal draws by formation method of the present invention from the CD assembly.HF-AC coupled signal from HF matrix amplifier 18A (Figure 21) in the head assemblies 174 (Figure 19) is directed into buffer amplifier card 152 (Figure 22 and 23).This signal is exaggerated and handles (Figure 24 B), is directed into the out connector J3 (Figure 22) of output 157 then.

HF-A coupled signal (Figure 21) from head assemblies 174 (Figure 19) is directed buffer amplifier card 152 (Figure 22 and 23).This signal is exaggerated and handles (Figure 24 B and 24C), is directed into the out connector J7 (Figure 22) of output 157 then.

HF-DC coupled signal from HF matrix amplifier 18A (Figure 21) in the head assemblies 174 (Figure 19) is directed into buffer amplifier card 152 (Figure 22 and 23).Send this signal to ADC 150 from buffer amplifier card 152, and handled and monitor 146 imagings (Fig. 9 A and 12) by PC 158.As mentioned above, remodeling PC 142 can replace one or more treating apparatus described herein.Figure 77 A, 77B and 77C explanation utilize the scanning of the head assemblies generation unlike signal of disk drive to provide the diagrammatic representation that can detect the investigation feature.

Figure 78 is the diagrammatic representation that concerns between Figure 78 A and the 78B.

Figure 78 A and 78B are the circuit diagrams of the amplifier stage of second embodiment, and it can be according to the invention process in the impact damper card shown in Figure 22 and 23.

Figure 78 A is the partial circuit synoptic diagram of buffer amplifier.Take out the simulation HF-AC signal of head assemblies 174 (Figure 19) from the contact pin 19 and 14 (Figure 22 and 23) of connector 155.Input signal transmission by input load resistor and voltage regulation capacitor just going between with equilibrium with negative the lead-in wire between ground unrest.Then, positive signal is fed into the operational amplifier that is fed the loop buffering.Amplifying signal is directed into the output load resistor two ends.

Take out the simulation HF-B signal of head assemblies 174 from the contact pin 17 and 16 (Figure 22 and 23) of connector 155.As mentioned above, this signal is exaggerated and cushions.

Take out the simulation HF-C signal of head assemblies 174 from the contact pin 15 and 18 (Figure 22 and 23) of connector 155.As mentioned above, this signal is exaggerated and cushions.

Take out the simulation HF-D signal of head assemblies 174 from the contact pin 13 and 20 (Figure 22 and 23) of connector 155.As mentioned above, this signal is exaggerated and cushions.

Figure 78 B is the partial circuit synoptic diagram of buffer amplifier.Amplifying signal HF-A, HF-B, HF-C and HF-D transmission is by loading resistor independently and make up.The signal of combination is fed into the negative input end of operational amplifier, the wherein variable positive signal of feed-in.Amplifying signal is fed the loop buffering, before being fed into the negative input end of another operational amplifier, is directed into the two ends of variable load resistors.Amplifying signal is fed the loop buffering, and is handled by the array of capacitors of belt coil.Signal after the processing is fed into the positive input terminal of operational amplifier, and it is fed the loop buffering.Then, amplifying signal is directed into the two ends of output load resistor and voltage regulation capacitor, and exports at the connector J6 of output 157 (Figure 22).

The photo bio dish that biological and chemical is measured

Discussed below is the application of the present invention in biological and chemical.In ordering is used, by detecting which probe the dna sample of adhesion is arranged, can determine the nucleotidyl sequence in the dna sample.In diagnostic application, screening is used for determining from the cdna sample of individuality whether this individuality has disease or hereditary disease procatarxis in predetermined probe's group.

The present invention makes up genome on microfluidic technology and the photo bio dish and protein groups to detect the investigation feature in the test sample book.With reference to Figure 79 A, 79B, 79C and 79D, moisture test sample book 252 is placed on the photo bio dish 250 or in the photo bio dish 250, and is driven by the microchannel on the special preparation face 256 254 to finish required test.Capillarity, the added pressure of external unit, and/or centrifugal force (that is the power on rotating shaft center or the center of curvature directed towards object in the curvilinear motion) affacts on the test sample book to realize and to catch contacting of probe 258.Nucleic acid probe technology can be applicable to detect genetic mutation and relevant mechanism, and cancer screening is determined the drug toxicity level, detects hereditary illness, detects infectious disease and genetic fingerprint.

In addition, the present invention is applicable to and mixes phase system to finish hybridization assays.Ginseng Figure 80 A, 80B, 80C and 80D mix to measure to relate to mutually and finish hybridization on solid phases, for example, thin nylon or nitrocellulose membrane 262.For example, measure and often relate to spin coated nitrocellulose 262 thin layers to the substrate 264 of biology dish 260, wherein utilize suction pipe 266 or similar device to be incorporated with the film of sample 268, denatured DNA or set up sub-thread molecule 270, fixed dna or RNA are to film, and utilize heterogeneous nucleic acid and/or protein 272 to make remaining film attachment saturated, can avoid analyte and indicator to adhere on the film in non-special mode.All these steps must be implemented before finishing actual hybridization.Carry out subsequently step again to realize the indicator pearl in hybridization and location trapping region or the target area.Then, utilize incident beam to detect indicator, discuss as reference Figure 79.

The photo bio dish can be used for analysis and mensuration and the pharmacogenetics in heredity and the protein groups field, the gene expression formula, and compound screening, toxicology, legal medical expert's research, mononucleotide pleomorphism (SNP) is analyzed, short series connection indicator (STR) and clinical/molecular diagnosis.

Indicator

Many chemistry, biochemistry and biologicall test rely on and bring out the variation of optical property in the concrete test sample book.This variation can take place afterwards or after detecting indicator in detection investigation feature itself (for example, haemocyte).The investigation feature because of too little can not be by CD drive under the situation of reading optical beam detection, there is the indicator of selection affinity (that is, with test sample book in the atom of different chemical composition of investigation feature or the tendency that compound reacts or makes up) to help to detect to the investigation feature.Indicator reacts, combination, or adhere to the investigation feature, thus make detectable color, chemiluminescence, luminous, or other discernible marks enter into the investigation feature.

The luminous two big classes that in form can be divided into, fluorescence and phosphorescence, it depends on the character of foment.Light emitting molecule can absorb the photon of a wavelength energy, and launches the energy of another wavelength subsequently.Luminous is because the electronics in incident radiation bump or the excited molecule causes.The Electron absorption incident radiation, and transit to higher quantum level from lower quantum level.When electronics was got back to lower ground state level, unnecessary energy discharged as photon.Because each indicator has its characteristics of luminescence, a plurality of labeled molecule can detect two or more investigation features in the same test sample simultaneously.Wherein each labeled molecule is added different indicator.

Except luminous, utilize the color spot technology of ELIA (ELISA) and golden mark can change the optical property of biological antigens material.For example,, virus infections may be pointed out,, the sedimental ELISA of visible color can be implemented to produce if there is antibody in order to test in the blood sample basis whether have antibody.With reference to Figure 81 A, 81B, 81C, 81D, 81E, and 81F, ELISA utilize the surface 280 of coating antibody 284 special antigens 282 to test.After this surface was exposed to blood sample basis 286, the antibody in the sample adhered to antigen.Close the adhesion of antibody 288 and the reaction generation sediment 290 relevant of enzyme and substrate with rear surface and certain enzyme yoke, therefore, allow the existence of antibody in the CD drive recognition sample with the antigen level of adhesion.More refer in detail to utilize sediment as the content of indicating mechanism at U.S. Provisional Patent Application No.60/292, open in 110, its title is " Surface Assembly for ImmobilizingDNA Capture Probes Using Pellets as Reporters in Genetic AssaysIncluding Optical Bio-Discs and Methods Relating Thereto ", application on May 18 calendar year 2001, and at U.S. Provisional Patent Application No.60/313, open in 917, its title is " Surface Assembly for Immobilizing DNA Capture Probes inGenetic Assays using Enzymatic Reactions to generate Signal inOptical Bio-Discs and Methods Relating Thereto ", August 21 calendar year 2001 application is incorporated in these two files that this is for reference.

With reference to Figure 82, the pearl based assays relates to and utilizes spherical particle or pearl 300 to change the optical property of biological antigens material 302.Coating has the chemosphere 304 of special affinity on the pearl 300 to investigation feature in the test sample book.With reference to Figure 83 A, 83B, 83C and 83D are when test sample book is encased in the CD 310 that comprises indicator pearl 300 (Figure 82) or on the CD 310 time, if there is investigation feature 312, then it adheres to indicator pearl 300.Investigation feature 312 also adheres to the special-purpose trapping agent 314 on CD 310 surfaces 316.According to the method, if there is the investigation feature in the biological solution, then this investigation feature becomes bonding agent, and pearl indicator 300 is adhered to the biological trapping agent 314 that coils on 310 surfaces 316.When the biology dish rotated in CD drive, the centrifugal force of formation was driven to the outward flange of dish to the pearl indicator 318 that does not adhere to, and the pearl indicator that adheres to still keeps being distributed on the panel of coating trapping agent.The pearl that utilizes CD reader can detect and quantize to adhere to.Relevant two pearl determination techniques also are disclosed in the unsettled U.S. Patent application No.09/997 of common transfer, in 741, its title is " Dual Bead Assays IncludingOptical Bio-discs and Methods Relating Thereto ", November 27 calendar year 2001 application is incorporated in it that this is for reference.

Indicator useful among the present invention comprises, but be not limited to the nucleotide sequence that synthetic or biological method is made, synthetic or coordination binding amino acid sequence that biological method is made, the enzyme reaction product, with plastic microsphere or pearl, for example, they are by latex, polystyrene or colloid gold particle are made, coating wherein is the biomolecule that given material is had affinity, for example, and the biotin molecule in the DNA thigh.Suitable coating compounds comprises that those are made by streptavidin or neutrality.Choose the pearl that these have suitable size, therefore, reading optical beam or inquiry light beam can " be seen " or detect the surface reflectivity that is caused by these particles and change in the CD drive.

In some embodiment related to the present invention, the indicator pearl adheres to panel surface by the DNA hybridization.With reference to Figure 84 and 85, catch probe 332 and paste panel surface 330, and signal probe 334 pastes indicator pearl 300 (Figure 82).Under the situation of hybridization assays, these two probes and target sequence 336 complementations.Under the situation that has target sequence 336, catch the hybridization of probe and signal probe and target.In this manner, indicator pearl 300 pastes panel surface 330.In centrifugal (or cleaning) step subsequently, remove the indicator pearl that all do not adhere to.Perhaps, under the situation that does not have the additional signaling probe, target itself directly adheres to or is connected to the indicator pearl.

With reference to Figure 86, under the situation of immunity, panel surface 340 coating acceptors 342 (for example, antibody), acceptor 342 adhere to required analyte 344 (for example, investigation feature) specially.The trapping region 346 of each concrete analysis thing to be determined can separate at the analysis area of dish.The acceptor 342 (being respectively antibody or antigen) that exists in the district 346 if analyte 344 (antigen or antibody) is hunted down is caught, and then the existence that combination can be used for analyte quantification takes place the signal of analyte special use.

Perhaps, if the investigation feature has the suitable size that incident beam detects in the CD drive, then it can not need indicator.The great changes that some chemical reaction and the product that forms thus or secondary product (that is sediment) bring out tested biological specimen optical property.This variation also can occur in and detect after the investigation feature, for example, is used to set up under the situation of micro-image in the present invention.The optical property of CD drive detection of biological panel surface changes, and sets up image based on this variation.

In a specific embodiment of the present invention, optical disk system (for example, Fig. 9 A and 12) comprising: signal processing system (for example, 142, or 158 and 156, or have and do not have 154 158 and 32, or have and do not have among Fig. 9 A 152 158 and 150) and the photo detector circuit of CD drive (for example, Fig. 1 18), it is configured to produce one at least from CD assembly (for example, the dish 130 of Figure 12) and carries information signal (for example, HF, TE, or FE signal).Signal processing system is coupled to photoelectric detector 18, can be from this at least one (for example carry operation information that information signal obtains being used for the operational light disc system, the tracking of Fig. 8, focus on and rate signal) with (for example point out the investigation feature relevant with the CD assembly, the investigation feature 68 of Figure 43) flag data of Cun Zaiing (for example, the track among Figure 61 B).

In a variation scheme of the present invention, the signal processing system of optical disk system comprises: PC and the analog to digital converter of digitized signal to PC be provided.Analog to digital converter is coupling in this, and at least one carries between information signal and the PC.PC comprises: program module is used for detecting and describing the peak value (for example, see among Figure 61 B track) of digitized signal.Preferably, PC also comprises: another program module is used for detecting and the bimodal number of count numbers signal (for example, see among Figure 61 B track D and E).

In a variation scheme of the present invention, the signal processing system of optical disk system comprises: PC, provide digitized signal to PC analog to digital converter and be coupling in analyzer 154 (embodiment III among Fig. 9 A) between analog to digital converter (for example, converter 32 among Fig. 8) and the PC.Analog to digital converter is coupling in this, and at least one carries between information signal and the PC.Analyzer comprises: the logic that is used for detecting and describing the digitized signal peak value.Preferably, analyzer also comprises: be used for detecting the logic bimodal with the count numbers signal.

In a variation scheme of the present invention, the signal processing system of optical disk system comprises: PC and the analog to digital converter of digitized signal to PC (embodiment IV among Fig. 9 A) is provided.Analog to digital converter is coupling in this, and at least one carries between information signal and the PC.Signal processing system also comprises: audio processing modules (for example, Fig. 9 A 156), it is coupling in this, and at least one carries between information signal and the analog to digital converter.Preferably, utilize predetermined sound that the CD assembly is carried out prerecord, and PC comprises: program module, when having the investigation feature, at least one carries the flag data of information signal and predetermined sound deviation to be used to detect this.In another variation scheme, predetermined sound is that coding is quiet.

In a variation scheme of the present invention, the signal processing system of optical disk system comprises: PC and the analog to digital converter of digitized signal to PC (embodiment II among Fig. 9 A) is provided.Analog to digital converter is coupling in this, and at least one carries between information signal and the PC.Signal processing system also comprises: be coupling in this at least one carry external buffer amplifier (for example, 152 among Fig. 9 A) between information signal and the analog to digital converter.

In a variation scheme of the present invention, the signal processing system of optical disk system comprises: PC and the analog to digital converter of digitized signal to PC is provided.Analog to digital converter is coupling in this, and at least one carries between information signal and the PC.Signal processing system also comprises: the detection trigger circuit (for example, 164 of Figure 12) that is coupled to analog to digital converter.The effect of detection trigger circuit is that at least one carries special time relevant when having flag data in the information signal with this in detection.

In another embodiment, signal processing system comprises: programmable digital signal processor, be configured to (1) selectively when first configuration, and at least one carries information signal and extracts operation information from this, or (2) are moved so that flag data to be provided as analog to digital converter when second configuration.For example, see the embodiment III of Figure 25 and Fig. 9 A.

In another embodiment, the signal processing system of optical disk system comprises: PC (for example, Fig. 9 A 158), be coupled to this at least one carry information signal programmable digital signal processor (for example, Fig. 9 A 32), and be coupling in analyzer (for example, 154 of Fig. 9 A) between programmable digital signal processor and the PC.Analyzer provides the flag data shown in the embodiment III of Fig. 9 A.

In another embodiment, the signal processing system of optical disk system comprises: detection trigger circuit (for example, Figure 12 164), the time cycle when its detects photo detector circuit scanning investigation feature relevant with the CD assembly.

In another embodiment, the signal processing system of optical disk system comprises: detection trigger circuit (for example, Figure 12 164), it detect this at least one carry special time when having flag data in the information signal.Exist the time of flag data periodically to take place in the information signal this at least one year.Special time is that (1) this at least one is carried and existed flag data to begin in the information signal or before schedule time when finishing, (2) meanwhile, or (3) schedule time after this.

In another embodiment, the signal processing system of optical disk system comprises: PC (for example, Fig. 9 A 158), and at least one carries audio processing modules (for example, 156 of Fig. 9 A) between the information signal to be coupling in PC and this.Preferably, audio processing modules is the external module that is independent of CD drive, as the Drive Module of part CD drive, or the remodeling Drive Module of part CD drive.In the variation scheme of this embodiment, PC comprises: be coupled to saved software module in the processor of sound module and the storer, can processor controls so that from voice data, extract flag data (for example, seeing the embodiment IV of Fig. 9 A).

In another embodiment, the photo detector circuit of optical disk system comprises: at least one carries the circuit of information signal as this to be used to produce simulating signal.Simulating signal comprises the high-frequency signal from photoelectric detector, tracking error signal, focus error signal, automatic gain control setting, recommend tracking signal, CD tracking signal, CD-R tracking signal, focus signal, differential phase detection device signal, laser power monitor device signal or voice signal.

In another embodiment, optical disk system also comprises: CD assembly (for example, 130 of Figure 12).The CD assembly has investigation feature relevant on the assembly that is arranged on the first dish sector (for example, Figure 12 136), and the operation information (for example, 133 of Figure 12) of the CD drive of encoding on the assembly of all the other dish sectors of operation is arranged.

In a variation scheme, the CD assembly comprises: triggered mark (for example, Figure 12 166), it is arranged on the CD assembly precalculated position with respect to the first dish sector.Signal processing system also comprises: the detection trigger circuit (for example, 164 of Figure 12) that detects triggered mark.Preferably, detection trigger circuit period property ground detects triggered mark, with based on triggered mark with respect to first the dish sector the precalculated position, (1) schedule time before when photo detector circuit is read relevant investigation feature, (2) meanwhile, or (3) schedule time afterwards detect triggered mark.

In a variation scheme, the relevant investigation feature of CD assembly comprises: the plastic microsphere of biomolecule coating is arranged, the colloid gold bead of biomolecule coating is arranged, silica bead, beaded glass, latex beads, magnetic bead, or fluorescent bead.

In another embodiment of the present invention, provide a kind of method, the method comprising the steps of: the deposition test sample book, rotary CD is inducted into irradiating light beam, detects Returning beam and handles the Returning beam that detects.The step of deposition test sample book comprises: the precalculated position deposition sample on the CD assembly.The step of rotary CD comprises: the CD assembly in the rotary CD driver.The step that is inducted into irradiating light beam comprises: lead beam is to the CD assembly.The step that detects Returning beam comprises: detect the Returning beam that incident beam and test sample book interact and form.Handling the step that detects Returning beam comprises: the Returning beam of handling detection is to obtain the information of the investigation feature relevant with test sample book.

In the variation scheme of this embodiment, the step that detects Returning beam forms a plurality of simulating signals.Handling the step that detects Returning beam comprises: first subclass of a plurality of simulating signals of suing for peace is to produce summing signal, make up first subclass of a plurality of simulating signals or second subclass to produce tracking error signal, from tracking error signal, obtain being used for the information of operational light disk drive, and summing signal is converted to digitized signal.

In another embodiment of the present invention, method of the present invention comprises step: obtain a plurality of simulating signals, first subclass of suing for peace makes up second subclass, obtains information, and summing signal is converted to digitized signal.The step that obtains a plurality of simulating signals utilizes a plurality of photoelectric detectors to obtain simulating signal from the CD assembly.The step of first subclass of suing for peace comprises that first subclass of a plurality of simulating signals of suing for peace is to produce summing signal.The step that makes up second subclass comprises that second subclass that makes up a plurality of simulating signals is to produce tracking error signal.The information step of obtaining comprises the information that obtains being used for the operational light disk drive from tracking error signal.

In a variation scheme, obtain step and summation step and produce the summing signal that comprises disturbance, it points out the investigation feature on the CD module position.

In another variation scheme, this method also comprises: describe the investigation feature based on digitized signal.

In another variation scheme of this method, switch process comprises: the CD drive chipset of configuration section moves as analog to digital converter.Preferably, configuration step comprises: the digital signal processing chip in the programming CD drive chipset moves as analog to digital converter.Preferably, digital signal processing chip comprises: normalization function, analog-digital converter function, demodulate/decode function, and output interface function.Preferably, configuration step also comprises: set up a paths from analog-digital converter function to the output interface function, the transmission summing signal is to avoid the demodulate/decode function.Preferably, configuration step also comprises: deactivation demodulate/decode function.

In another variation scheme of this method, switch process comprises: configuration comprises the normalization function, analog-digital converter function, the demodulate/decode function, and the digital signal processing chip of output interface function, and configuration step comprises: set up a paths from analog-digital converter function to the output interface function, make summing signal not by the demodulate/decode function treatment.Preferably, configuration step comprises: deactivation demodulate/decode function.

In another embodiment of the present invention, a kind of method comprises step: transform the signal processing system of part, obtain a plurality of simulating signals, and converting analogue signals, and based on digitized signal description investigation feature.The step of transforming the part signal disposal system comprises: transform this part and move as analog to digital converter.The step that obtains a plurality of simulating signals comprises from the photo detector circuit of CD drive and obtains simulating signal.A plurality of simulating signals comprise the information of pointing out investigation feature on the CD assembly.The step of converting analogue signals comprises utilizes signal processing system that analog signal conversion is become digitized signal.Preferably, adaptation step comprises: the digital signal processing chip in the programming signal disposal system is the analog to digital converter operation.

In another embodiment of the present invention, a kind of method that comprises receiving step and switch process.Receiving step comprises: at the corresponding input end of signal processing circuit, receive each signal at least one simulating signal.This at least one simulating signal is provided by at least one corresponding photodetector unit, and photodetector unit detects the light that returns from the CD assembly surface.Switch process comprises: this at least one analog signal conversion is become corresponding digitized signal.Each digitized signal is proportional to the back light intensity that this at least one photodetector unit detects basically.

In the variation scheme of this embodiment, switch process comprises: the operation signal treatment circuit is with any demodulation of bypass first digitized signal.Preferably, switch process also comprises: the operation signal treatment circuit is with any decoding of bypass first digitized signal, and the operation signal treatment circuit is with any inspection of mistake in bypass first digitized signal.

In another variation scheme of this embodiment, switch process comprises: the operation signal treatment circuit is with any decoding of bypass first digitized signal.

In another variation scheme of this embodiment, switch process comprises: the operation signal treatment circuit is with any inspection of mistake in bypass first digitized signal.

In another variation scheme of this embodiment, this method also comprises step: make up at least two signals in this at least one simulating signal.Preferably, combination step is to be selected from step in the following step: add, subtract, remove, multiply by and their combination.Preferably, combination step is to carry out before switch process.Perhaps, combination step can be carried out after switch process.

In another variation scheme, the method comprising the steps of: after switch process, provide first digitized signal of this at least one digitized signal at the output interface of signal processing circuit, its is not basic to change switch process and first digitized signal between the step is provided.Preferably, signal processing circuit comprises digital signal processor.Preferably, signal processing circuit is to be made of digital signal processor.

The material that uses in the inventive method is the preparation that is particularly suitable for external member.This external member can comprise separated carriage, places photo bio dish and one or more container in confined conditions, for example, bottle, pipe, or the like, each container comprises the independent element that uses in this method.For example, a container can comprise the adhesive agent of indicator and/or protein special use, for example, and antibody.Another container can comprise the nucleic acid of isolation, antibody, and protein, and/or reagent described herein, this is known or following exploitation in this area.As required, these compositions can be liquid or water wettability form.The antibody that uses in the analysis kit of the present invention can be monoclonal or polyclonal antibody.For convenience, we can also provide the indicator that is bonded to biological dish substrate.In addition, indicator can also make up with the mark (for example, radioactive label or enzyme) that uses in the analytical approach of future exploitation.Typical external member also comprises one group of instruction using in the said method.

In the variation scheme of this embodiment, carriage can also be separated into and comprise the optical disc apparatus that contains software, is used for to biology dish configuration computing machine.Randomly, external member can be packaged together with the remodeling CD drive.For example, the purpose that external member can be used to educate, it is as a kind of selection of simple microscope.

Though description of the invention is with reference to some preferred embodiments, should be appreciated that the present invention is not limited to these specific embodiments.On the contrary, consider to describe and put into practice the disclosure that the current optimal mode of the present invention provides that under the condition that does not depart from the scope of the invention and spirit, this area professional can do many changes and variation to it.So scope of the present invention is following claims, rather than content described above.In the meaning of claims and all improvement in the scope, change and variation all should belong to scope of the present invention.

At length and according to the requirement of Patent Law describe the present invention, the content that we ask patent protection is in following appending claims.

Claims

1. optical disk system comprises:

Objective lens unit is used to transmit into the CD of irradiating light beam to the air plane of incidence that carries an investigation feature at least;

The photo detector circuit of CD drive is configured to produce one at least from the CD assembly and carries information signal;

Be coupled to the signal processing system of photo detector circuit, can from described at least one carry information signal and obtain being used for the operation information of operational light disc system and point out the flag data that described investigation feature exists; With

Be coupled to the adjustment lens of outgoing position on the described objective lens unit, described incident beam is reflected, thereby allow described optical disk system to read described CD.

2. according to the optical disk system of claim 1, wherein said signal processing system also comprises: testing agency, it utilizes the signal that AGC (automatic gain control) system produces in the described signal processing system.

3. according to the optical disk system of claim 1, wherein said signal processing system also comprises: testing agency, the signal that it utilizes described optical disk system inner focusing servo control mechanism to produce is used to detect the existence of the above investigation feature of described CD.

4. according to the optical disk system of claim 1, wherein said signal processing system also comprises: testing agency, it utilizes the signal that tracking servo produces in the described optical disk system, is used to detect the existence of the above investigation feature of described CD.

5. according to the optical disk system of claim 1, wherein said signal processing system also comprises: testing agency, the signal that it utilizes described optical disk system internal power control gear to produce is used to detect the existence of the above investigation feature of described CD.

6. according to the optical disk system of claim 1, wherein said signal processing system also comprises: testing agency, the signal that it utilizes described optical disk system inter-sync mechanism to produce is used to detect the existence of the above investigation feature of described CD.

7. according to the optical disk system of claim 1, wherein said signal processing system also comprises: testing agency, it utilizes the signal that Code And Decode mechanism produces in the described signal processing system, is used to detect the existence of investigation feature on the described CD.

8. according to the optical disk system of claim 1, wherein said signal processing system also comprises:

The channels bits generator wherein produces known good data position and adds the data bit that receives from described year information signal to produce a position summation; With

Block decoder sends to described block decoder to institute's rheme summation, wherein can obtain background block error information.

9. according to the optical disk system of claim 1, also comprise: prism is used to measure the birefringence in described CD district.

10. according to the optical disk system of claim 1, wherein said signal processing system also comprises: the look-up table of optimization is used to detect described investigation feature.

11. the optical disk system according to claim 1 also comprises: solid immersion lens.

12. according to the optical disk system of claim 1, wherein signal processing system comprises: programming logic, the existence that is used for reading the demoder error code of described optical disk system and detects described investigation feature.

13. according to the optical disk system of claim 1, wherein said CD is the backswing Photo CD.

14. according to the optical disk system of claim 1, wherein said CD comprises the embedded data mask, is used to strengthen described investigation and studies carefully the error signal that feature causes.

15. according to the optical disk system of claim 1, wherein said signal processing system also comprises: the detection trigger circuit, be used to detect the monomark on the described CD, wherein said monomark is utilized beginning and the end of auto-correlation sign indicating number with the detection trigger cycle.

16. one kind changes optical disk system to detect the method for investigation feature on the CD, may further comprise the steps:

Change the circuit of described optical disk system, wherein operating characteristics is used to detect described investigation feature.

17., also comprise step: implement the access at random on swinging chute broadcast and the swinging chute according to the method for claim 16.

18., also comprise step: add inquiry and monitoring the laser monitoring value, wherein insert the reading that the laser power monitor detecting device detects in the light picker of described optical disk system according to the method for claim 16.

19., also comprise step: add the automatic gain control value that inquires in the described optical disk system according to the method for claim 16.

20., also comprise step: add inquiring the tracking automatic gain control value according to the method for claim 16.

21., also comprise step: add monitor to the C1 demoder according to the method for claim 16.

22., also comprise step: add monitor to the C2 demoder according to the method for claim 16.

23., also comprise step: add the steering logic of going beyond one's commission, wherein said optical disk system initialization and the operating characteristics of following the tracks of on the described dish that is independent of codimg logic according to the method for claim 16.

24. according to the method for claim 16, also comprise step: add steering logic, wherein the user can utilize special speed and the described optical disk system of laser read-out power initialization.

25. according to the method for claim 16, also comprise step: stream transmission comprises main channel and the sub-channel data of introducing and drawing described CD.

26. according to the method for claim 16, also comprise step: interpolation is used to release the electric wire of original EFM value to port, and wherein the user can see 14 bit data before converting 8 place values to.

27. according to the method for claim 16, also comprise step: add electric wire and make additional treatments to release a data set to outside port, described data set comprises buffering signals, DC coupled signal, TE, FE and HF signal.

28., also comprise step: add and to be used to decode and to inquire about when initialization the mechanism of collecting numerical value from power calibration district and described CD program storage district according to the method for claim 16.

29., also comprise step: suspend the broadcast of described CD and open tracking servo with monitoring open-loop tracking signal according to the method for claim 16.

30. according to the method for claim 16, also comprise step: illusory initialization logic is set, and wherein said optical disk system can be configured to skip the reading catalogue listing from described CD.

31., also comprise step: interactive following function of turn-offing described optical disk system according to the method for claim 16.

32. according to the method for claim 16, also comprise step: the focusing that utilizes described optical disk system is with the described CD district of heating up.

33., also comprise step: utilize the EEPROM on the described optical disk system to pack new logic into to described optical disk system according to the method for claim 16.

34. one kind changes the DVD optical disk system to detect the method for investigation feature on the DVD CD, may further comprise the steps:

35., also comprise step: exchange each layer on the described DVD CD according to the method for claim 34.

36., also comprise step: utilize in the described DVD optical disk system laser to read CD or CD-RW CD according to the method for claim 34.

37., also comprise step: utilize laser in the described DVD optical disk system with any frequency-tracking swinging chute according to the method for claim 34.

38., also comprise step: add monitoring the buffering differential phase detection signal value that described DVD optical disk system follower produces according to the method for claim 34.

39. an optical analysis disc used when imaging biology or medical science investigation feature comprises:

Substrate,

The operation layer relevant with described substrate layer, described operation layer has the operation information of coding, and wherein said operation layer comprises the swinging chute magnetic track from described optical analysis disc center convolution; With

Investigation feature with respect to described operation layer placement, described operation layer has the optics chosen or magnetism characteristic so that form predetermined contrast between them, point out the return signal distinguished between information that described operation layer is relevant and the described investigation feature thereby provide, wherein said swinging chute is configured to the isolation of tracking signal and HF or quaternary summing signal.

40. a multilayer optical analysis disc used when imaging biology or medical science investigation feature comprises:

The operation layer that encoding operation information is arranged, wherein said operation layer comprise the swinging chute magnetic track from described optical analysis disc center convolution; With

Paste the big second layer of described operation layer, the described second layer has the investigation feature, and described operation layer has the optics chosen or magnetism characteristic so that form predetermined contrast between them, points out the return signal distinguished between information that described operation layer is relevant and the described investigation feature thereby provide.