CN1480206A - Combination of gadol and Chinese caterpillar fungus as well as preparing method - Google Patents
Combination of gadol and Chinese caterpillar fungus as well as preparing method Download PDFInfo
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- CN1480206A CN1480206A CNA021336865A CN02133686A CN1480206A CN 1480206 A CN1480206 A CN 1480206A CN A021336865 A CNA021336865 A CN A021336865A CN 02133686 A CN02133686 A CN 02133686A CN 1480206 A CN1480206 A CN 1480206A
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- cordyceps
- radix rhodiolae
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- mycelium
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Abstract
A radiation-resistant Chinese medicine for improving immunity is prepared from rhodiola root and cordyceps through respective extracting and proportionally mixing.
Description
The present invention relates to a kind of is the raw material combination composition formula with Radix Rhodiolae and Cordyceps, and said composition has radioprotective, immune enhancing function.
Radix Rhodiolae belongs to Crassulaceae, and rhodiola (Rhodiola L.) plant is a class natural medicinal plant that is grown under 3500 meters-5000m of the height above sea level extreme environment, and its main medicinal part is a root and rhizome.The medicines and health protection effect of Radix Rhodiolae is just on the books as far back as the classical Four-Volume Medical Code of Tibetan medicine and Compendium of Material Medica, discover both at home and abroad that in recent years rhodioside is the effective constituent that mainly contains in the Radix Rhodiolae extract, experiment and clinical data show: Radix Rhodiolae extract has pharmacological action widely, mainly contain: (1) increases raying bone marrow nucleated cell number and survival rate, have tangible radiation resistance (2) and improve blood rbc oxygen carrier amount, alleviate and eliminate various fatigue symptoms, improve the efficient of muscle power and mental work.
Cordyceps, (Cordyceps sinensis (Berk.) Sacc.) colonizes in Stroma and larva cadaveric complex on the Vespertilionidae insect larvae, the separated and artificial culture of its mycelium for section ergot fungus cordyceps sinensis.Further investigation through medical expert of China and foreign countries and biologist is found: Cordyceps is the nourishing healthy treasure-house of a mystery, and it contains most nutritions of needed by human body, and its main effective component is that cordycepin is a ribosidoadenine.The pharmacological action of Cordyceps mainly shows: (1) immunomodulating and immune enhancing function: the significantly effect of activated mononuclear macrophage and macrophage, obviously improve Turnover of Mouse Peritoneal Macrophages phagocytic index and phagocytic percentage, the inhibition of antagonism cortisone induced mice peritoneal macrophage phagocytic function, strengthen identification, processing and the transmission capacity of macrophage by macrophage surface Fc acceptor number purpose is increased, and realize target cell ADCC effect by the Fc receptor to antigenic information; Promote spleen lymphocyte proliferation, improve spleen lymphocyte E-Flos Rosae Rugosae formation rate, and the inhibition of obvious antagonism meticortelone of energy and the formation of caused by cyclophosphamide mouse T lymphocyte E-rosette; Promote the lymphocyte mitosis, the propagation of T, bone-marrow-derived lymphocyte is had significant induced activity; Significantly strengthen mouse spleen NK activity, and the obviously active inhibition of antagonism caused by cyclophosphamide NK.(2) promote hemopoietic function: Cordyceps has significant promotion hematosis.Obviously improve the periodic state of mouse bone marrow cells hematopoietic stem cell (CFU-S), promote CFU-S propagation.The effectively inhibition of antagonism harringtonine induced mice bone marrow CFU-E (CFU-E); Promote mice spleen megakaryocyte proliferation differentiation and maturation, increased platelets counts, and can significantly protect
60The platelet injury of Co radiation gamma induced mice reduces the platelet count fall, makes the platelet ultrastructure keep normal substantially.
The object of the present invention is to provide a kind of is the raw material combination composition formula with Radix Rhodiolae and Cordyceps, with the effect of the radioprotective immunostimulant that improves Radix Rhodiolae and Cordyceps.
The present invention is according to the result of Chinese medical theory in conjunction with experimentation, Radix Rhodiolae extract and Cordyceps extract or the pure powder of Cordyceps mycelium are prepared resulting composition by a certain percentage, or water and ethanol extraction resulting composition again after Radix Rhodiolae and Cordyceps mixed by a certain percentage, broken through the limitation of folk prescription Radix Rhodiolae and folk prescription Cordyceps, its produce effects is rapider, it is more powerful lasting to act on: (1) has obvious radiation resistance (2) and obviously promotes the synthetic effect of erythrocyte (3) to significantly improve the human immunoglobulin level, strengthens immune function of human body.
The present invention also aims to provide described Radix Rhodiolae and Cordyceps preparation of compositions method.
Radix Rhodiolae involved in the present invention and Cordyceps preparation of compositions method comprise: Radix Rhodiolae and Cordyceps mix the back water by a certain percentage and ethanol extraction prepares this compositions; The preparation of Radix Rhodiolae extract, Cordyceps extract preparation, Radix Rhodiolae extract and Cordyceps extract or Cordyceps mix according to a certain percentage this compositions; Said composition can be made into capsule, tablet, oral liquid or granule.
Radix Rhodiolae of the present invention and Cordyceps preparation of compositions technology are as follows:
(1) preparation of Radix Rhodiolae extract: with the root and rhizome of Radix Rhodiolae is to be ground into coarse powder behind the raw material chip drying, the coarse powder alcohol reflux of 50-75%, extracting liquid filtering, concentrate, be drying to obtain Radix Rhodiolae extract, this extract is used petroleum ether, chloroform, ethyl acetate, n-butanol extraction successively, can obtain the Radix Rhodiolae extract of purification.
(2) preparation of Cordyceps extract: Cordyceps or Cordyceps mycelium were fried in shallow oil and are carried 2-3 time after being soaked in water 2-4 hour, and each 2-3 hour, behind the extracting liquid filtering, concentrating under reduced pressure was drying to obtain Cordyceps extract.
(3) Radix Rhodiolae and Cordyceps preparation of compositions: after Radix Rhodiolae and Cordyceps mixed according to a certain percentage respectively water and ethanol extraction, concentrate, drying can make this compositions; Or with Radix Rhodiolae extract and Cordyceps extract or the pure powder of worm summer in winter mycelium mix by a certain percentage this compositions, said composition can be made into capsule, tablet, oral liquid or granule; Acute toxicity testing:
Kunming mouse, body weight 20-22g; The SD rat, body weight 182-220g, each 10 of male and female, test dose is 10g/kg.bw, irritate the stomach amount: every mice is pressed 0.02ml/10g.bw, and rat is pressed 2.0ml/10g.bw, and per os was irritated stomach twice, two minor tick 2 hours, after irritating stomach, observed for two weeks, toxic reaction and the death condition of record animal.
Per os acute toxicity testing animal varieties sex quantity approach dosage death as a result LD to mice and rat
50Conclusion
(g/kg.bw) male 10 per os 10.0 0＞10.0 of number of animals (g/kg.bw) Kunming mouse are actual nontoxic
Male 10 per os 10.0 0＞10.0 of female 10 per os, 10.0 0＞10.0 actual nontoxic SD rats are actual nontoxic
Female 10 per os 10.0 0＞10.0 are actual nontoxic
The result shows: after each test dose group per os is irritated stomach, do not have special poisoning manifestations, no animal dead in two weeks.Kunming mouse and SD rat peroral acute toxicity belong to actual non-toxic type all greater than 10g/kg.bw, so Radix Rhodiolae of the present invention and Cordyceps compositions are safety non-toxics.
Radioprotective Ergonomy test: select female Kunming mouse, be divided into matched group, radiation matched group and this compositions test group at random.The test group animal is irritated stomach every day once, totally 30 days by 10,20,30 multiple doses of recommended dose 1.0g/ people/day (16.66mg/kg.bw) (166.6,333,500mg/kg.bw) respectively.Blank group and radiation matched group are irritated stomach with distilled water every day.The 3rd week was used radiation matched group and three test group animals after irritating stomach
60The Co gamma-rays shines, once a day, 1.0Gy/ time, totally 10 times.Radiation length: 80cm.Absorbed dose 0.01cGy/min.Get blood in 5 days after the last radiation and put to death a collection of zoometry peripheral white blood cell (WBC), get femur bone marrow and measure the nucleated cell number, get bone marrow of sternum by in " toxicological evaluation of food safety procedure and method ", " micronucleus test ' the method film-making of regulation, dyeing, microscopy; Another batch animal gives over to observes after the radiation for the first time 30 days survival rates and mean survival time.
This compositions is to the influence of mice peripheral white blood cell, bone marrow nucleated cell number
Group dosage number of animals leukocyte count nucleated cell number
(mg/kg.bw) (only) * 10
9/ L * 10
6/ L
Blank 0.0 12 7.88 ± 1.28 18.51 ± 5.72
Radiation contrasts 0.0 12 0.53 ± 0.25
*1.14 ± 0.41
*
Compositions 166.66 12 0.25 ± 0.10
*▲ 1.34 ± .38
*
333.3 12 0.20±0.07
*▲?1.88±0.66
*▲
500.0 12 0.21±0.09
*▲?2.43±1.05
*▲
*P＜0.01 (comparing) with the blank group; ▲: P＜0.01 (comparing) with the radiation matched group
Influence to the mouse Bone marrow cells micronucleus rate
Group dosage number of animals is had a liking for many RBC number micronucleus and is counted micronuclear rates
(mg/kg.bw) (only) (individual/only) (individual) ‰
Blank 0.00 10 1,000 1.91 ± 1.1 1.9
Radiation contrasts 0.00 10 1,000 23.1 ± 3.8* 23.1
Compositions 166.6 10 1,000 12.7 ± 3.8* ▲ 12.7
333.3 10 1000 13.0±3.6*▲ 13.0
500.0 10 1000 7.8±2.1*▲ 7.8
*P＜0.01 (comparing) with the blank group; ▲: P＜0.01 (comparing) with the radiation matched group
Influence to 30 days survival rates after the mice raying
Group dosage is observed 30 days surviving animals of number of animals and is counted survival rate
(mg/kg.bw) (only) (only) (%)
Blank 0.0 15 15 100.0
Radiation contrasts 0.0 15 0 0.0
*
Compositions 166.6 15 2 13.3
*
333.3 15 0 0.0
*
500.0 15 4 26.7
*▲
*P＜0.001 (comparing) with the blank group; ▲: P＜0.05 (comparing) with the radiation matched group
The result shows: the pure compound powder of Radix Rhodiolae extract and Cordyceps mycelium can make raying mouse bone marrow cells nucleated cell number increase (P＜0.01), 30 days survival rates increase (P＜0.05) bone marrow polychromatic erythrocyte micronuclear rates and reduce (P＜0.01), have obvious radiation resistance.
Human experimentation
Experimental subject: 20 of middle-distance runners, be the male, be divided into two groups at random according to height and body weight: (1) experimental group 10 people, (every contains Radix Rhodiolae extract 180mg to take six of the capsules of being made by the present composition every day, the pure powder 120mg of Cordyceps mycelium), took for 4 week continuously.(2) matched group 10 people take equivalent blank product.Experimental session two groups of experimenters all normally train.
Blood cell analysis: carried out extracting venous blood on an empty stomach morning the same day of carrying out exercise test when experiment beginning and end, places Lithium acid heparin anticoagulant test tube.Measure erythrocyte numeration (RBC), hemoglobin concentration (KBG) and hematid specific volume (HCT) with ADVIA-120 type whole blood cell analysis instrument (Bayer Corp., USA).
Serum immune globulin is measured: measure G type immunoglobulin (IgG), A type immunoglobulin (IgA) and M type immunoglobulin (IgM) content with turbidimetry.Determining instrument is KEYSYS, reagent: Luo Shi reagent.
The blood cell analysis result
Group RBC counting HB HCT
(×10
12cells/l) (g/l) (l/l)
Matched group experiment preceding 4.61 ± 0.196 148.7 ± 7.07 0.41 ± 0.03
(n=10) the experiment back 4.66 ± 0.175 151.1 ± 5.38 0.42 ± 0.02
Experimental group experiment preceding 4.59 ± 0.208 139.2 ± 9.37 0.40 ± 0.02
(n=10) the experiment back 4.81 ± 0.178
*165.4 ± 6.00
*0.48 ± 0.03
Significant difference, P＜0.05 are arranged before and after taking
Matched group experimenter's red blood cell count(RBC), hemoglobin and hematid specific volume are compared after the experiment with before the experiment, all no significant difference.Experimental group is subjected to envoy's red blood cell count(RBC), hemoglobin and hematid specific volume, compares after the experiment with before the experiment, and notable difference (P＜0.05) is all arranged.The result shows: take this compositions the experimenter is had the synthetic effect of significant promotion erythrocyte.
The serum immune globulin test result
Group IgA IgG IgM
(g/l) (g/l) (g/l)
Matched group experiment preceding 1.08 ± 0.21 1.72 ± 0.38 1.33 ± 0.21 (n=10) experiment back 1.06 ± 0.18 1.64 ± 0.19 1.38 ± 0.38
Experimental group experiment preceding 0.96 ± 0.16 1.05 ± 0.18 1.39 ± 0.21
(n=10) the experiment back 1.65 ± 0.25
*1.80 ± 0.45
*1.46 ± 0.56
Significant difference, P＜0.05 are arranged before and after taking
Experimental group and matched group experimenter's serum IgM level is compared after the experiment with before the experiment, does not have significant difference.Experimental group experimenter's IgA, the IgG level is compared after the experiment with before the experiment, all has significance to improve (P＜0.05).The result shows: take this compositions and can significantly improve experimenter's immunoglobulin level.
Embodiment:
Radix Rhodiolae root and rhizome drying, cut into slices, be ground into coarse powder, get the 1KG coarse powder, divide four times, each 4000ml with 75% ethanol, leaching in 30 minutes, concentrating under reduced pressure reclaims ethanol, gets the about 1200g of concentrated solution, and the distilled water of reuse equivalent stirs evenly, leave standstill, filtration treatment repeatedly, concentrating under reduced pressure gets concentrated aqueous liquid, the concentrated aqueous liquid drying can be directly Radix Rhodiolae extract.This extract can be further purified with petroleum ether, chloroform, ethyl acetate, n-butanol extraction successively.Utilize high performance liquid chromatography to determine in Radix Rhodiolae extract and the pure powder of Cordyceps mycelium the content of main effective component rhodioside and adenosine.With Radix Rhodiolae extract 180mg (containing rhodioside 4.2mg), the pure powder 120mg of Cordyceps mycelium (containing adenosine 0.18mg) is mixed and made into capsule at last.Promptly get a kind of in the compositions of the present invention.
Claims (9)
1. the invention provides a kind of is the raw material combination composition formula with Radix Rhodiolae and Cordyceps.
2. said composition is to be mixed according to a certain percentage by Radix Rhodiolae extract and Cordyceps extract or the pure powder of Cordyceps mycelium according to claim 1.
3. said composition is to mix back water and ethanol extraction by a certain percentage by Radix Rhodiolae and Cordyceps to be prepared from according to claim 1.
4. the percentage by weight of each component is Radix Rhodiolae extract: 10-90% in the compositions as claimed in claim 1, and the pure powder of Cordyceps extract or Cordyceps mycelium is 10-90%.
5. Radix Rhodiolae as claimed in claim 1 and Cordyceps compositions are a kind of in capsule, tablet, oral liquid and the granule.
6. the preparation method of Radix Rhodiolae extract as claimed in claim 2, its feature comprise the Radix Rhodiolae root and rhizome are dried and crushed into coarse powder; Coarse powder 50-75% ethanol extraction, extracting solution gets Radix Rhodiolae extract through concentrated, dry, and this extract can be further purified with organic solvent extraction.
7. organic solvent as claimed in claim 6 is petroleum ether, chloroform, ethyl acetate, n-butyl alcohol.
8. Radix Rhodiolae extract as claimed in claim 2 comprises the Radix Rhodiolae extract that obtains from 50-75% ethanol extract concentrate drying; The Radix Rhodiolae extract that this extract is obtained with the organic solvent extraction purification maybe.
9. Cordyceps extract preparation method as claimed in claim 2 is characterized by: with Cordyceps and Cordyceps mycelium be soaked in water, fry in shallow oil carry, filtration, concentrating under reduced pressure be drying to obtain Cordyceps extract.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA021336865A CN1480206A (en) | 2002-09-02 | 2002-09-02 | Combination of gadol and Chinese caterpillar fungus as well as preparing method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA021336865A CN1480206A (en) | 2002-09-02 | 2002-09-02 | Combination of gadol and Chinese caterpillar fungus as well as preparing method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104352779A (en) * | 2014-10-11 | 2015-02-18 | 西藏彩轮藏药有限公司 | Traditional Chinese medicine composition containing cordyceps sinensis and preparation method of traditional Chinese medicine composition |
| CN104383262A (en) * | 2014-10-28 | 2015-03-04 | 甘南吉祥香巴拉旅游产品有限责任公司 | Capsule for enhancing immune function |
| CN105079076A (en) * | 2014-05-13 | 2015-11-25 | 泰宗生物科技股份有限公司 | Pharmaceutical composition for treating growth hormone insufficiency syndrome in adults |
-
2002
- 2002-09-02 CN CNA021336865A patent/CN1480206A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105079076A (en) * | 2014-05-13 | 2015-11-25 | 泰宗生物科技股份有限公司 | Pharmaceutical composition for treating growth hormone insufficiency syndrome in adults |
| CN104352779A (en) * | 2014-10-11 | 2015-02-18 | 西藏彩轮藏药有限公司 | Traditional Chinese medicine composition containing cordyceps sinensis and preparation method of traditional Chinese medicine composition |
| CN104352779B (en) * | 2014-10-11 | 2017-09-19 | 西藏彩轮藏药有限公司 | Chinese medicine composition containing cordyceps sinensis and preparation method thereof |
| CN104383262A (en) * | 2014-10-28 | 2015-03-04 | 甘南吉祥香巴拉旅游产品有限责任公司 | Capsule for enhancing immune function |
| CN104383262B (en) * | 2014-10-28 | 2017-06-06 | 甘南吉祥香巴拉旅游产品有限责任公司 | A kind of capsule for strengthening immunologic function |
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