CN1456540A - High-performance composite biological nuisanceless granular fertilizer and producing method thereof - Google Patents
High-performance composite biological nuisanceless granular fertilizer and producing method thereof Download PDFInfo
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Abstract
An efficient composite granular biologic fertilizer is prepared from nitrogen-fixing bacteria, organic phosphorus bacteria, growth factor, organic substance (city garbage and leftover of agricultural and subsidary products), inorganic substance, and trace elements. Its advantages are high fertility and low cost.
Description
The present invention relates to a kind of efficient biological compound fertilizer and production method thereof, belong to a kind of employing biological nitrogen fixation bacterium, phosphorus bacteria, potassium bacterium and organic raw material, inorganic raw material, trace element cooperation, the short long factor of preferred best compatibility and making or interpolation farm crop is made the high-performance bio compounded granular fertilizer through novel process.
Have many pieces of patent documents to disclose all production methods of utilizing bio-bacterial manure to produce compound manure in the prior art, wherein immediate documents is:
Publication number 1188093A discloses and has utilized black liquid or fermented waste fluid to prepare bio-bacterial manure, cooperates with inorganic raw material to produce a kind of organic-biological compound fertilizer, and its shortcoming is: the one, and bacterial classification and bacterial manure are made not exclusively open; The 2nd, select for use inorganic raw material prescription and kind indeterminate.
Publication number 1235138A discloses and has utilized yeast and biological bacterium fertilizer, cooperates with inorganic raw material to produce a kind of high-effect organic biological composite fertilizer, and its shortcoming is: the one, and the type of bacterial classification and bacterial classification quantity are too much, are difficult to realize in producing and making.
Publication number 1203211A discloses and has utilized domestic waste to make raw material, cooperates with inorganics, bio-bacterial manure to produce a kind of organic-biological compound fertilizer, and its shortcoming is: the one, and the title of bio-bacterial manure and numbering and technology are not open, can't make bio-bacterial manure; The 2nd, there is not example.
It is not enough to the objective of the invention is to overcome prior art processes design and prescription, a kind of high-effect organic biological all element compound fertilizer and production method thereof are provided, this method adopts with cooperations such as organic raw material, bio-bacterial manure, inorganicss, particularly utilize municipal wastes organic matter, agricultural byproducts offal and various cake, animal byproducts (skin, hair, bone) etc., can effectively realize the comprehensive utilization of refuse, turn waste into wealth.
The efficient biological compound fertilizer and the production method thereof of present technique invention comprise following step:
1. the preparation of raw material comprises mutagenesis, separation, the purifying of bacterial classification; The making of microbial inoculum and bacterial manure substratum and fermentation; The preparation of fertilizer and making; The preparation of inorganic fertilizer and prescription; The preparation and the making of trace element; The prescription of composite fertilizer and technology are:
(1) mutagenesis of bacterial classification, separation, purifying;
(2) making and the zymotechnique of bacteria agent and bacterial manure substratum;
(3) substratum of bio-bacterial manure, sterilization, inoculation, fermentation;
(4) preparation of fertilizer and making comprise humic acid fertilizer and fertile two classes of organic matter material;
(5) preparation of inorganic fertilizer and prescription;
(6) trace element is allocated into:
Its cooperation ratio is:
1. sal epsom 0.5-2.5,2. lime carbonate 0.5-2.5,3. boric acid 0.05-0.15,4. zinc sulfate 0.15-0.25,5. manganous sulfate 0.25-0.35,6. ammonium molybdate 0.005-0.015,7. copper sulfate 0.5-0.15,8. Xanthohumate 0.15-0.25,9. rare earth 0.04-0.095;
(7) prescription of high-performance bio compounded granular fertilizer and preparation:
1. AS1.824Azotbacter uinelandii Wei Nielande vinelandii and AS1.223 BaciLLus megaterium lift Bacterium anthracoides and AS4.891Steptomyces miroftauus streptomyces microflavus; Or
AS1.824Azotbacter uinelandii Wei Nielande vinelandii and AS1.910 BaciLLus muciLaginosus bacillusmusilaginosiengineering and the AS1.867Pseudomonas fturescens Pseudomonas fluorescens bacterial manure amount of allocating into are: 30-60%
2. the amount of allocating into of inorganic raw material is: 30-60
Nitrogen, phosphorus, potassium content proportioning are: 3-8: 2-6: 1-5
Urea content is 46.6%, adds (mass parts) to be: 5-19 part;
Phosphorus content is 14%, and add-on (mass parts) is 20-50;
KCE content is 58%, and add-on (mass parts) is: 6-14 part;
3. the fertilizer amount of allocating into (mass parts) is: 30-60
Humic acid fertilizer 15-30
The fertile 15-30 of organic matter material
4. the trace element amount of allocating into (mass parts) is: 2-8 part.Its cooperation ratio is:
Sal epsom 0.5-2.5, lime carbonate 0.5-2.5, boric acid 0.05-0.15, zinc sulfate 0.15-0.25, manganous sulfate 0.25-0.35, ammonium molybdate 0.005-0.015, copper sulfate 0.5-0.15, Xanthohumate 0.15-0.25, rare earth 0.04-0.095
5. make mixing granulation:
Elder generation is with fertilizer and humic acid phosphate fertilizer adds in the mixing machine or adding flyash; adding bio-bacterial manure again stirs; thereafter adding inorganics mixes; the composite fertilizer's batch mixing that mixes is arrived the tablets press granulation by conveyor delivery; air stream drying or oven drying at low temperature promptly become the complete first compounded granular fertilizer of high-effect organic biological:
In the present invention, biological composite bacterial manure adopts agricultural byproducts offal or excrement of animals or the municipal wastes organism substratum as bio-bacterial manure, and add of the short long agent of a spot of phosphate fertilizer as biology, preferably resolve the growth processing environment pollution problem preferably again that to satisfy biological bacteria, make bio-bacterial manure in compound manure, play synergy.Wherein, the bacillus megaterium organophosphorus bacterium bacterium that can resolve into available phosphorus to the organophosphorus of invalid attitude in the soil or inorganic phosphorus; Bacillusmusilaginosiengineering bacterial fertilizer, it can decompose the potassium in the mineral out, can obtain the plain nutrition of phosphorus again from hard phosphorus ash, can also be from air fixing nitrogen, can strengthen the crop disease-resistant ability simultaneously; The Wei Nielande vinelandii can be not changed into by the nitrogen that farm crop utilize in the air by the nitrogen nutriment of crop absorption; Streptomyces microflavus can produce yield stimulant and antibiotic, farm crop is had diseases prevention, keeps a full stand of seedings, loosens the soil and stimulate multiple effects such as plant growth; Mineral manure has quick-acting to farm crop, and they participate in the vital movement of crop in every way, plays a part constituent and metabolic activities compound in the vegetable cell; AS1.867Pseudomonas fturescens Pseudomonas fluorescens bacterial manure, the inorganic phosphorus that can decompose invalid attitude is an available phosphorus; Trace element has single-minded physiological action in plant-growth, they interdepend in the whole growth process of plant, condition each other, and are in a kind of equilibrium state; Fertilizer can make full use of agricultural and sideline product offal and municipal wastes organism, reduces fertilizer cost, and can be the carrier that the existence of biological bacteria in compound manure is played protection and nutrition is provided.
High-performance bio compounded granular fertilizer of the present invention and production method thereof have the following advantages and effect:
1. adopt farm crop by product, municipal wastes organic matter, animal skin offal, increased the organic matter and the proteic content in soil, reduced hardening of soil, for the growth of bio-bacterial manure provides good cultivation material and nutriment;
2. adopt phosphorus decomposing, potassium bacterium fertilizer, the absorption that has improved organic and inorganics phosphorus transforms, and has played mutual synergy;
3. adopt the cooperation of bio-bacterial manure, organic matter, mineral manure, trace element, improved fertilizer efficiency, reduced the utilization of inorganic fertilizer, saved raw material, reduced cost;
4. effectively utilize farm crop by product and animal excrement and municipal wastes organic matter, refuse is turned waste into wealth, simultaneously, improved environment, its economic benefit and social benefit are quite remarkable;
5. be rich in biologically active substance and somatomedin, farm crop had the effect that stimulates growth, preventing disease and pest.
Every technical indicator of present technique invention design meets relative national standards, through field experiment, has all obtained good effect.
The high-performance bio compounded granular fertilizer that the by product of present technique design employing municipal wastes organism, crop products, animal excrement, inorganics, trace element etc. design and produce, compare with inorganic fertilizer, price has reduced 15-30%, and fertilizer efficiency has improved 10-20%.
Used following bio-bacterial manure and organic, micro-in the present technique invention, wherein, biological inoculum is to buy from China Committee for Culture Collection of Microorganisms common micro-organisms center and behind resurrection, mutagenesis, separation, purifying, chooses the high reactivity bacterial strain as producing bacterial classification; Mineral manure nitrogen, phosphorus, potassium and trace element need to buy from market; The organic need collected or purchased from relevant department, handles through fermentation and oven dry.
Be divided into several steps below and introduce mutagenesis, separation, the purifying that the present technique invention comprises bacterial classification respectively; The making of microbial inoculum and bacterial manure substratum and fermentation; The preparation of fertilizer and making; The preparation of inorganic fertilizer and prescription; The preparation and the making of trace element; The prescription of composite fertilizer and technology:
Wherein the bacterial classification of biological composite bacterial manure is:
(1) AS1.824Azotbacter uinelandii Wei Nielande vinelandii; (2) AS1.223 BaciLLusmegaterium lifts Bacterium anthracoides; (3) AS1.910 BaciLLus muciLaginosus bacillusmusilaginosiengineering; (4) AS1.867Pseudomonas fturescens Pseudomonas fluorescens; (5) AS4.891Steptomyces miroftauus streptomyces microflavus.
1. the mutagenesis of bacterial classification, separation, purifying are:
(1) preparation bouillon media;
(2) bouillon media was sterilized 30 minutes in 1.5 kg/cm;
(3) under aseptic technique, get the above bacterial classification of a ring and put into bouillon media;
(4) micro oscillation juice substratum, the streptomyces antibioticus of adding trace makes it and spares;
(5) will pour in the culture dish with the substratum of even sample to be separated, shake up;
(6) uviolizing is handled and purebred cultivation.
Opened the UV-lamp preheating earlier 15 minutes, make light wave stable, then culture dish is inserted darkroom or sterilisable chamber, from about UV-lamp 15-25 centimetre, locating to shine 1-3 minute (deciding) on the active different of bacterial classification, open red light or other colored lights simultaneously, after irradiation finishes, culture dish wrapped up the culture dish of handling with black cloth or other dark cloth insert in the incubator, under 28-30 ℃ of constant temperature, cultivated 24-72 hour, it is vigorous to select growth, active high bacterial strain inserts in the test tube slant substratum to be cultivated, mutagenesis so repeatedly, separate, screening, there is not assorted bacterium until from test tube or culture dish, filtering out, energetic effective bacterial classification inserts in the slant tube then as producing bacterial classification or making the vacuum lyophilization peace bottle normal phase of pipe and preserve.
2. the making of microbial inoculum substratum and fermentation:
(1) making of AS1.824Azotbacter uinelandii Wei Nielande fixed nitrogen bacterial classification and microbial inoculum:
1. the making of slant strains substratum: potassium primary phosphate kh
2PO
40.02-0.025 gram, dipotassium hydrogen phosphate k
2HpO
40.08-0.085 gram, sal epsom Mgso
4.7h
2O 0.020-0.025 gram, calcium sulfate CaSO
4.2h
2O 0.01-0.015 gram, iron(ic) chloride feCl
3Trace, Sodium orthomolybdate NaMoO
4.2h
2The O trace, yeast extract 0.050-0.10 gram, N.F,USP MANNITOL 2-2.5 gram, agar 1.5-2 gram, distilled water 100-150 milliliter, ph value 7.2;
2. secondary microbial inoculum solid medium (mass parts) is: potassium primary phosphate kh
2PO
40.01-0.02 gram, dipotassium hydrogen phosphate k
2HpO
4O 0.04-0.08 gram, sal epsom Mgso
4.7h
2O 0.01-0.02 gram, calcium sulfate CaSO
4.2h
2O 0.005-0.01 gram, yeast extract 0.001-0.05 gram, N.F,USP MANNITOL 0.2-2 gram, starch 1-2 gram, cotton seed cake 40-60 gram, loam 40-80 gram, calcium superphosphate 0.5-1.5 part, the peat composed of rotten mosses or plant ash or flyash 3-6 regulate about ph value 7-7.5 with unslaked lime;
Medium sterilization, inoculation and cultural method are:
Medium sterilization: above slant medium, secondary microbial inoculum solid medium culture material are mixed, slant medium is packed in vitro, all the other secondary mediums are put into Cans or triangular flask or cloth bag, oral area or top are with cotton or multilayer gauze or kraft paper gag, substratum is put into or is inserted Autoclave, with 1.5 kg/cm pressure sterilizations 30 minutes;
Culture medium inoculated and cultural method are: when treating that substratum cools to 40 ℃ of following temperature, insert female bacterial classification or activation peace bottle dry freeze pipe bacterial classification by aseptic technique test tube slant substratum, secondary medium inserts inclined-plane or triangular flask bacterial classification, temperature is controlled at about 25-280 ℃, fermentation time generally was controlled at 48-72 hour, and microbial inoculum can be as the production microbial inoculum of bacterial manure after microscopy is qualified.
(2) making of AS1.223 BaciLLus megaterium bacillus megaterium bacterial manure:
1. the making of slant strains: peptone 0.5-1.5 gram, beef extract 0.1-0.5 gram, sodium-chlor 0.1-0.9 gram, agar 1.5 grams, 100 milliliters of distilled water, ph value 7.0;
2. second class inoculum solid medium (mass parts) is: peptone 0.2-0.6, beef extract 0.1-0.3, sodium-chlor 0.1-0.3, rice bran (Mai Fu or cotton seed cake) 20-60, crop stalk powder 20-40, loam 20-40, calcium superphosphate 0.5-1.5 part, the peat composed of rotten mosses or plant ash or flyash 3-6 regulate about ph value 7-7.5 with unslaked lime;
Medium sterilization, inoculation and cultural method are:
Medium sterilization: above slant medium, secondary microbial inoculum solid medium culture material are mixed, slant medium is packed in vitro, all the other secondary mediums are put into Cans or triangular flask or cloth bag, oral area or top are with cotton or multilayer gauze or kraft paper gag, substratum is put into or is inserted Autoclave, with 1.5 kg/cm pressure sterilizations 30 minutes;
Culture medium inoculated and cultural method are: when treating that substratum cools to 40 ℃ of following temperature, insert female bacterial classification or activation peace bottle dry freeze pipe bacterial classification by aseptic technique test tube slant substratum, secondary medium inserts inclined-plane or triangular flask bacterial classification, temperature is controlled at about 25-28 ℃, fermentation time generally was controlled at 48-72 hour, and microbial inoculum can be as the production microbial inoculum of bacterial manure after microscopy is qualified.
(3) making of AS1.910 BaciLLus muciLaginosus bacillusmusilaginosiengineering kind microbial inoculum:
1. the making of slant strains: potassium primary phosphate kh
2Po
40.02-0.04 gram, dipotassium hydrogen phosphate k
2HpO
40.04-0.10 gram, sal epsom MgSO
4.7h
2O 0.01`-0.02 gram, calcium sulfate Ca
4.SO
42h
2O 0.005-0.012 gram, iron(ic) chloride feCl
3Trace, molybdic acid are received Na
2MoO
4.2h
2The o trace, yeast extract 0.05 gram, N.F,USP MANNITOL 2 grams, agar 1.5 grams, 100 milliliters of distilled water, ph value 7.2;
2. second class inoculum solid medium (mass parts) is: potassium primary phosphate kh
2Po
40.01-0.02 gram, dipotassium hydrogen phosphate k
2HpO
40.04-0.10 gram, sal epsom MgSO
4.7h
2O 0.01-0.02 gram, calcium sulfate CaSO
4.2h
2O 0.005-0.015 gram, rice bran (wheat bran or cotton seed cake) 20-60, crop stalk powder 20-40, loam 20-40, calcium superphosphate 0.5-1.5 part, the peat composed of rotten mosses or plant ash or flyash 3-6 regulate about ph value 7-7.5 with unslaked lime;
Medium sterilization, inoculation and cultural method are:
Medium sterilization: above slant medium, secondary microbial inoculum solid medium culture material are mixed, slant medium is packed in vitro, all the other secondary mediums are put into Cans or triangular flask or cloth bag, oral area or top are with cotton or multilayer gauze or kraft paper gag, substratum is put into or is inserted Autoclave, with 1.5 kg/cm pressure sterilizations 30 minutes;
Culture medium inoculated and cultural method are: when treating that substratum cools to 40 ℃ of following temperature, insert female bacterial classification or activation peace bottle dry freeze pipe bacterial classification by aseptic technique test tube slant substratum, secondary medium inserts inclined-plane or triangular flask bacterial classification, temperature is controlled at about 25-28 ℃, fermentation time generally was controlled at 48-72 hour, and microbial inoculum can be as the production microbial inoculum of bacterial manure after microscopy is qualified.
(4) making of AS1.867Pseudomonas fturescens Pseudomonas fluorescens kind and microbial inoculum:
1. the making of slant strains: peptone 1 gram, beef extract 0.3 gram, sodium-chlor 0.5 gram, agar 1.5 grams, 100 milliliters of distilled water, ph value 7.0;
2. second class inoculum solid medium (mass parts) is: peptone 0.2-0.6, beef extract 0.1-0.3, sodium-chlor 0.1-0.3, rice bran (wheat bran or cotton seed cake) 20-60, crop stalk powder 20-40, loam 20-40, calcium superphosphate 0.5-1.5 part, the peat composed of rotten mosses or plant ash or flyash 3-6 regulate about ph value 7-7.5 with unslaked lime;
Medium sterilization, inoculation and cultural method are:
Medium sterilization: above slant medium, secondary microbial inoculum solid medium culture material are mixed, slant medium is packed in vitro, all the other secondary mediums are put into Cans or triangular flask or cloth bag, oral area or top are with cotton or multilayer gauze or kraft paper gag, substratum is put into or is inserted Autoclave, with 1.5 kg/cm pressure sterilizations 30 minutes;
Culture medium inoculated and cultural method are: when treating that substratum cools to 40 ℃ of following temperature, insert female bacterial classification or activation peace bottle dry freeze pipe bacterial classification by aseptic technique test tube slant substratum, secondary medium inserts inclined-plane or triangular flask bacterial classification, temperature is controlled at about 25-28 ℃, fermentation time generally was controlled at 48-72 hour, and microbial inoculum can be as the production microbial inoculum of bacterial manure after microscopy is qualified.
(5) making of AS4.891Steptomyces miroftauus streptomyces microflavus bacterial manure:
1. the making of slant strains: Zulkovsky starch 2 grams, lime carbonate KNO
31 gram, dipotassium hydrogen phosphate k
2HpO
40.05 gram, sodium-chlor NaCI 0.05 gram, ferric sulfate FeSO
4.7H
2O 0.001 gram, agar 1.5 grams, 100 milliliters of distilled water, ph value 7.2-7.4;
2. dipotassium hydrogen phosphate k
2HpO
40.05 gram, sal epsom MgSO
4.7H
2O 0.05 gram, lime carbonate KNO
30.5 gram, soybean cake powder 20-40 part, loam 50-80 part, straw powder 25-50, calcium superphosphate 0.5-1.5 part, the peat composed of rotten mosses or plant ash or flyash 3-6 regulate about ph value 7-7.5 with unslaked lime; Medium sterilization, inoculation and cultural method are:
Medium sterilization: above slant medium, secondary microbial inoculum solid medium culture material are mixed, slant medium is packed in vitro, all the other secondary mediums are put into Cans or triangular flask inner bag or cloth, oral area or top are with cotton or multilayer gauze or kraft paper gag, substratum is put into or is inserted Autoclave, with 1.5 kg/cm pressure sterilizations 30 minutes;
Culture medium inoculated and cultural method are: when treating that substratum cools to 40 ℃ of following temperature, insert female bacterial classification or activation peace bottle dry freeze pipe bacterial classification by aseptic technique test tube slant substratum, secondary medium inserts inclined-plane or triangular flask bacterial classification, temperature is controlled at about 25-28 ℃, fermentation time generally was controlled at 48-72 hour, and microbial inoculum can be as the production microbial inoculum of bacterial manure after microscopy is qualified.
3. the making of bacterial manure and substratum and fermentation:
Wherein the compatibility of bacterial manure is:
AS1.824Azotbacter uinelandii Wei Nielande vinelandii and AS1.223 BaciLLus megaterium lift Bacterium anthracoides and AS4.891Steptomyces miroftauus streptomyces microflavus; Or
AS1.824Azotbacter uinelandi i Wei Nielande vinelandii and AS1.910 BaciLLus muciLaginosus bacillusmusilaginosiengineering and AS1.867Pseudomonas fturescens Pseudomonas fluorescens:
(1) substratum, sterilization, inoculation, the fermentation of AS1.824Azotbacter uinelandii Wei Nielande vinelandii and AS1.223 BaciLLus megaterium act Bacterium anthracoides and the bio-bacterial manure of AS4.891Steptomyces miroftauus streptomyces microflavus are:
Dipotassium hydrogen phosphate k
2HpO
40.01-0.05 part, potassium primary phosphate kh
2Po
40.01-0.02 part, sal epsom MgSO
4.7H
2O0.01-0.05 part, soya-bean cake 2-5 part, crop stalk powder 3-8 part, wheat bran 2-5 part, calcium superphosphate 0.5-1.5 part, flyash or peat composed of rotten mosses 5-10 part, loam or organic 50-73 part, adjusting the ph value with lime is 7.5-8, and humidity is adjusted to about 30-40%, normal-pressure sterilization 2-3 hour or autoclaving 30 minutes, when treating that material cools to the 40 degree left and right sides, insert 2 grades of microbial inoculums, the access amount is 1-10%, insert after stirring in fermentation machine or fermentation frame or fermentation vat or the fermentation dish, shallow-layer (20 centimetres) or deep ventilation fermentation about control material temperature 30 degree, are not stirred, cultivated 3-5 days, and promptly can be high-quality bacterial manure;
(2) substratum, sterilization, inoculation, the fermentation of AS1.824Azotbacter uinelandii Wei Nielande vinelandii and AS1.910 BaciLLusmuciLaginosus bacillusmusilaginosiengineering and AS1.867Pseudomonas fturescens Pseudomonas fluorescens bacterial manure are:
Potassium primary phosphate kh
2Po
40.01-0.02 part, dipotassium hydrogen phosphate k
2HpO
40.04-0.02 gram, sal epsom Mgso
4.7h
2O0.01-0.02 part, 5 parts of soya-bean cakes, 8 parts in crop stalk powder, 5 parts in wheat bran, 1 part of calcium superphosphate, 8 parts of the flyash or the peats composed of rotten mosses, 73 parts of loam or organic matters, adjusting the ph value with lime is 7.5-8, and humidity is adjusted to about 30-40%, normal-pressure sterilization 2-3 hour or autoclaving 30 minutes, when treating that material cools to the 40 degree left and right sides, insert 2 grades of microbial inoculums, the access amount is 1-10%, insert after stirring in fermentation machine or fermentation frame or fermentation vat or the fermentation dish, shallow-layer (20 centimetres) or deep ventilation fermentation about control material temperature 30 degree, are not stirred, cultivated 3-5 days, and promptly can be high-quality bacterial manure;
4. the preparation of fertilizer and making:
(1) humic acid fertilizer is prepared and is made:
1. weathered coal or peat or peat composed of rotten mosses 60-100 part are ready to;
2. take by weighing 20 parts of dilute sulphuric acid proportions about 1.28, mix with an amount of water;
3. weathered coal or peat or the peat composed of rotten mosses are mixed with the mixture of dilute sulphuric acid and water, stir acidification 24-48 hour;
4. the weathered coal after the acidification or peat or the peat composed of rotten mosses and 15-30 part ground phosphate rock or phosphate fertilizer mixing and stirring, reaction 5-6 days of banking up is humic-acid kind phosphate fertilizer.
(2) organic matter material fertilizer (bag Chinese juniper municipal wastes or animal excrement and fur offal or agricultural byproducts offal and various biscuit):
1. the residue of municipal wastes under residue after the magnetic separation stage treatment through banking up more than spontaneous fermentation 8-15 days, passes through 10-30 mesh sieve again, and filtrate is planted matter for organic and corruption, is good organic fertilizer after drying or drying;
2. add part grass meal or saw foam or weathered coal soil in the animal excrement, through spontaneous fermentation in 5-10 days or add biological organophosphorus bacterial fertilizer three-class strain fermentation 3-5 days, be good organic fertilizer after drying or drying;
3. the animal skin offal was pulverized 10-30 mesh sieve, added bio-azotobacter fertilizer material microbial inoculum and organophosphorus bacterial fertilizer or secondary bacteria fermentation 4-6 days, was good organic protein fertilizer after drying or drying.
5. the preparation of inorganic fertilizer and prescription:
Nitrogen, phosphorus, potassium content proportioning are: 3-8: 2-6: 1-5
1. urea content is 46.6%, adds (mass parts) to be: 5-19 part;
2. phosphorus content is 14%, and add-on (mass parts) is 20-50;
3. KCE content is 58%, and add-on (mass parts) is: 6-14 part.
6. Wei Liangyuansu preparation and making: the trace element amount of allocating into (mass parts) is: 2-8 part, and its cooperation ratio is:
1. sal epsom 0.5-2.5,2. lime carbonate 0.5-2.5,3. boric acid 0.05-0.15,4. zinc sulfate 0.15-0.25,5. manganous sulfate 0.25-0.35,6. ammonium molybdate 0.005-0.015,7. copper sulfate 0.5-0.15,8. Xanthohumate 0.15-0.25,9. rare earth 0.04-0.095;
7. the prescription of composite fertilizer and technology:
(1) AS1.824Azotbacter uinelandii Wei Nielande vinelandii and AS1.223 BaciLLus megaterium lift Bacterium anthracoides and AS4.891Steptomyces miroftauus streptomyces microflavus; Or
AS1.824Azotbacter uinelandi i Wei Nielande vinelandii and AS1.910 BaciLLus muciLaginosus bacillusmusilaginosiengineering and the AS1.867Pseudomonas fturescens Pseudomonas fluorescens bacterial manure amount of allocating into (mass parts) are: 10-60
(2) amount of allocating into of inorganic raw material (mass parts) is: 30-60
Nitrogen, phosphorus, potassium content proportioning are: 3-8: 2-6: 1-5
1. urea content is 46.6%, adds (mass parts) to be: 5-19 part;
2. phosphorus content is 14%, and add-on (mass parts) is 20 1 50;
3. KCE content is 58%, and add-on (mass parts) is: 6-14 part.
(3) the fertilizer amount of allocating into (mass parts) is: 30-60
1. humic acid fertilizer 15-30
2. the fertile 15-30 of organic matter material
(4) the trace element amount of allocating into (mass parts) is: 2-8 part, and its cooperation ratio is:
1. sal epsom 0.5-2.5,2. lime carbonate 0.5-2.5,3. boric acid 0.05-0.15,4. zinc sulfate 0.15-0.25,5. manganous sulfate 0.25-0.35,6. ammonium molybdate 0.005-0.015,7. copper sulfate 0.5-0.15,8. Xanthohumate 0.15-0.25,9. rare earth 0.04-0.095;
8. high-performance bio compounded granular fertilizer example 1
(1) compatibility of bio-bacterial manure and the amount of allocating into are: 40 parts
Wherein: AS1.824Azotbacter uinelandii Wei Nielande vinelandii 10 (parts) and AS1.223 BaciLLusmegaterium lift 10 parts of Bacterium anthracoides) and AS4.891Steptomyces miroftauus streptomyces microflavus 20 (part):
(2) amount of allocating into of inorganic raw material (mass parts) is 29
Nitrogen, phosphorus, potassium content proportioning are: 3: 2.5: 2
1. urea content is 46.6%, adds (mass parts) to be: 6.5;
2. phosphorus content is 14%, and add-on (mass parts) is 18;
3. KCE content is 58%, and add-on (mass parts) is: 4.5
(3) the fertilizer amount of allocating into (mass parts) is: 27
1. the humic acid fertilizer amount of allocating into (mass parts) is: 10
2. the organic matter material fertilizer amount of allocating into (mass parts) is: 17
(4) the trace element amount of allocating into (mass parts) is: 4 parts: its cooperation ratio is:
1. sal epsom 2, and 2. lime carbonate 2, and 3. boric acid 0.1, and 4. zinc sulfate 0.2, and 5. manganous sulfate 0.3, and 6. ammonium molybdate 0.01, and 7. copper sulfate 0.8, and 8. Xanthohumate 0.02, and 9. rare earth 0.1;
9. high-performance bio compounded granular fertilizer example 2
(1) compatibility of bio-bacterial manure and the amount of allocating into are 10 (parts)
AS1.824Azotbacter uinelandii Wei Nielande vinelandii (mass parts) 3 and AS1.910 BaciLLusmuciLaginosus bacillusmusilaginosiengineering (mass parts) 3 and the AS1.867Pseudomonas fturescens Pseudomonas fluorescens bacterial manure amount of allocating into (mass parts) are 4:
(2) amount of allocating into of inorganic raw material (mass parts) is: 59
Nitrogen, phosphorus, potassium content proportioning are: 6: 5: 4
1. urea content is 46.6%, adds (mass parts) to be: 13;
2. phosphorus content is 14%, and add-on (mass parts) is 36;
3. KCE content is 58%, and add-on (mass parts) is: 10
(3) the fertilizer amount of allocating into (mass parts) is: 27
1. the humic acid fertilizer amount of allocating into (mass parts) is: 10
2. the organic matter material fertilizer amount of allocating into (mass parts) is: 17
(4) the trace element amount of allocating into (mass parts) is: 4 parts: its cooperation ratio is:
1. sal epsom 2, and 2. lime carbonate 2, and 3. boric acid 0.1, and 4. zinc sulfate 0.2, and 5. manganous sulfate 0.3, and 6. ammonium molybdate 0.01, and 7. copper sulfate 0.8, and 8. Xanthohumate 0.02, and 9. rare earth 0.1;
10. mix and granulation
Make mixing granulation:
Elder generation is with fertilizer and humic acid phosphate fertilizer adds in the mixing machine or adding flyash, adds bio-bacterial manure again and stirs, and adds inorganics thereafter and mixes, and the composite fertilizer's batch mixing that mixes is arrived the tablets press granulation by conveyor delivery; Be total to granulation perhaps earlier inorganic fertilizer and fertilizer elder generation mixing granulation, and then with bio-bacterial manure.Air stream drying or oven drying at low temperature promptly become the high-performance bio compounded granular fertilizer.
Claims (7)
1. biological compounded granular fertilizer of a Nuisanceless high-efficient and production method thereof comprise mutagenesis, separation, the purifying of bacterial classification; The making of bacterial classification and microbial inoculum substratum and fermentation; The making of bacterial manure substratum and fermentation; The preparation of fertilizer and making; The preparation of inorganic fertilizer and prescription; The preparation and the making of trace element; The prescription of composite fertilizer and technology;
Wherein the bacterial classification of biological composite bacterial manure is:
(1) AS1.824Azotbacter uinelandii Wei Nielande vinelandii: (2) ASl.223 Bacillusmegaterium lifts Bacterium anthracoides: (3) ASl.910 Bacillus mucilaginous bacillusmusilaginosiengineering: (4) AS1.867Pseudomonas fturescens Pseudomonas fluorescens: (5) AS4.891Steptomyces miroftauus streptomyces microflavus;
2. biological compounded granular fertilizer of a kind of Nuisanceless high-efficient according to claim 1 and production method thereof is characterized in that mutagenesis, separation and the purifying of above-mentioned bacterial classification:
(1) preparation bouillon media;
(2) bouillon media was sterilized 30 minutes in 1.5 kg/cm;
(3) under aseptic technique, get the above bacterial classification of a ring and put into bouillon media;
(4) micro oscillation bouillon media, the streptomyces antibioticus of adding trace makes it and spares;
(5) will pour in the culture dish with the substratum of even sample to be separated, shake up;
(6) uviolizing is handled and purebred cultivation;
Opened the UV-lamp preheating earlier 15 minutes, make light wave stable, then culture dish is inserted darkroom or sterilisable chamber, from about UV-lamp 15-25 centimetre, locating to shine 1-3 minute (deciding) on the active different of bacterial classification, open red light or other colored lights simultaneously, after irradiation finishes, culture dish wrapped up the culture dish of handling with black cloth or other dark cloth insert in the incubator, under 28-30 ℃ of constant temperature, cultivated 24-72 hour, it is vigorous to select growth, active high bacterial strain inserts in the test tube slant substratum to be cultivated, mutagenesis so repeatedly, separate, screening, there is not assorted bacterium until from test tube or culture dish, filtering out, energetic effective bacterial classification inserts in the slant tube then as producing bacterial classification or making the vacuum lyophilization peace bottle normal phase of pipe and preserve;
3. biological compounded granular fertilizer of a kind of Nuisanceless high-efficient according to claim 1 and production method thereof is characterized in that the making of above-mentioned biological inoculum and microbial inoculum substratum and zymotechnique are:
(1) making of AS1.824Azotbacter uinelandii Wei Nielande fixed nitrogen bacterial classification and microbial inoculum:
1. the making of slant strains substratum: potassium primary phosphate kh
2PO
40.02-0.025 gram, dipotassium hydrogen phosphate k
2HpO
40.08-0.085 gram, sal epsom Mgso
4.7h
2The o0.020-0.025 gram, calcium sulfate CaSO
4.2h
2The o0.01-0.015 gram, iron(ic) chloride feCl
3Trace, Sodium orthomolybdate NaM
0O
4.2h
2The O trace, yeast extract 0.050-0.10 gram, N.F,USP MANNITOL 2-2.5 gram, agar 1.5-2 gram, distilled water 100-150 milliliter, ph value 7.2;
2. secondary microbial inoculum solid medium (mass parts) is: potassium primary phosphate kh
2PO
40.01-0.02 gram, dipotassium hydrogen phosphate k
2HpO
4O 0.04-0.08 gram, sal epsom Mgso
4.7h
2O 0.01-0.02 gram, calcium sulfate CaSO
4.2h
2O 0.005-0.01 gram, yeast extract 0.001-0.05 gram, N.F,USP MANNITOL 0.2-2 gram, starch 1-2 gram, cotton seed cake 40-60 gram, loam 40-80 gram, calcium superphosphate 0.5-1.5 part, the peat composed of rotten mosses or plant ash or flyash 3-6, regulate about ph value 7-7.5 with unslaked lime:
Medium sterilization, inoculation and cultural method are:
Medium sterilization: above slant medium, secondary microbial inoculum solid medium culture material are mixed, slant medium is packed in vitro, all the other secondary mediums are put into Cans or triangular flask or cloth bag, oral area or top are with cotton or multilayer gauze or kraft paper gag, substratum is put into or is inserted Autoclave, with 1.5 kg/cm pressure sterilizations 30 minutes:
Culture medium inoculated and cultural method are: when treating that substratum cools to 40 ℃ of following temperature, insert female bacterial classification or activation peace bottle dry freeze pipe bacterial classification by aseptic technique test tube slant substratum, secondary medium inserts inclined-plane or triangular flask bacterial classification, temperature is controlled at about 25-28 ℃, fermentation time generally was controlled at 48-72 hour, and microbial inoculum promptly can be used as the production microbial inoculum of bacterial manure after microscopy is qualified;
(2) making of AS1.223 Bacillus megaterium bacillus megaterium kind or microbial inoculum:
1. the making of slant strains: peptone 0.5-1.5 gram, beef extract 0.1-0.5 gram, sodium-chlor 0.1-0.9 gram, agar 1.5 grams, 100 milliliters of distilled water, ph value 7.0;
2. second class inoculum solid medium (mass parts) is: peptone 0.2-0.6, beef extract 0.1-0.3, sodium-chlor 0.1-0.3, rice bran (wheat bran or cotton seed cake) 20-60, agricultural crop straw powder 20-40, loam 20-40, calcium superphosphate 0.5-1.5 part, the peat composed of rotten mosses or plant ash or flyash 3-6 regulate about ph value 7-7.5 with unslaked lime;
Medium sterilization, inoculation and cultural method are:
Medium sterilization: above slant medium, secondary microbial inoculum solid medium culture material are mixed, slant medium is packed in vitro, all the other secondary mediums are put into Cans or triangular flask or cloth bag, oral area or top are with cotton or multilayer gauze or kraft paper gag, substratum is put into or is inserted Autoclave, with 1.5 kg/cm pressure sterilizations 30 minutes; Culture medium inoculated and cultural method are: when treating that substratum cools to 40 ℃ of following temperature, insert female bacterial classification or activation peace bottle dry freeze pipe bacterial classification by aseptic technique test tube slant substratum, secondary medium inserts inclined-plane or triangular flask bacterial classification, temperature is controlled at about 25-28 ℃, fermentation time generally was controlled at 48-72 hour, and microbial inoculum promptly can be used as the production microbial inoculum of bacterial manure after microscopy is qualified;
(3) making of AS1.910 Bacillus muciLaginosus bacillusmusilaginosiengineering microbial inoculum:
1. the making of slant strains: potassium primary phosphate kh
2Po
40.02-0.04 gram, dipotassium hydrogen phosphate k
2HpO
40.04-0.10 gram, sal epsom MgSO
4.7h
2The o0.01`-0.02 gram, calcium sulfate Ca
4.SO
42h
2The o0.005-0.012 gram, iron(ic) chloride feCl
3Trace, molybdic acid are received Na
2MoO
4.2h
2The o trace, yeast extract 0.05 gram, N.F,USP MANNITOL 2 grams, agar 1.5 grams, 100 milliliters of distilled water, ph value 7.2;
2. second class inoculum solid medium (mass parts) is: potassium primary phosphate kh
2Po
40.01-0.02 gram, dipotassium hydrogen phosphate k
2HpO
40.04-0.10 gram, sal epsom MgSO
4.7h
2The o0.01-0.02 gram, calcium sulfate CaSO
4.2h
2The o0.005-0.015 gram, rice bran (wheat bran or cotton seed cake) 20-60, agricultural crop straw powder 20-40, loam 20-40, calcium superphosphate 0.5-1.5 part, the peat composed of rotten mosses or plant ash or flyash 3-6, regulate about ph value 7-7.5 with unslaked lime:
Medium sterilization, inoculation and cultural method are:
Medium sterilization: above slant medium, secondary microbial inoculum solid medium culture material are mixed, slant medium is packed in vitro, all the other secondary mediums are put into Cans or triangular flask or cloth bag, oral area or top are with cotton or multilayer gauze or kraft paper gag, substratum is put into or is inserted Autoclave, with 1.5 kg/cm pressure sterilizations 30 minutes:
Culture medium inoculated and cultural method are: when treating that substratum cools to 40 ℃ of following temperature, insert female bacterial classification or activation peace bottle dry freeze pipe bacterial classification by aseptic technique test tube slant substratum, secondary medium inserts inclined-plane or triangular flask bacterial classification, temperature is controlled at about 25-28 ℃, fermentation time generally was controlled at 48-72 hour, and microbial inoculum promptly can be used as the production microbial inoculum of bacterial manure after microscopy is qualified;
(4) making of AS1.867Pseudomonas fturescens Pseudomonas fluorescens kind or microbial inoculum:
1. the making of slant strains: peptone 1 gram, beef extract 0.3 gram, sodium-chlor 0.5 gram, agar 1.5 grams, 100 milliliters of distilled water, ph value 7.0;
2. second class inoculum solid medium (mass parts) is: peptone 0.2-0.6, beef extract 0.1-0.3, sodium-chlor 0.1-0.3, rice bran (Mai Fu or cotton seed cake) 20-60, agricultural crop straw powder 20-40, loam 20-40, calcium superphosphate 0.5-1.5 part, the peat composed of rotten mosses or plant ash or flyash 3-6, regulate about ph value 7-7.5 with unslaked lime:
Medium sterilization, inoculation and cultural method are:
Medium sterilization: above slant medium, secondary microbial inoculum solid medium culture material are mixed, slant medium is packed in vitro, all the other secondary mediums are put into Cans or triangular flask or cloth bag, oral area or top are with cotton or multilayer gauze or kraft paper gag, substratum is put into or is inserted Autoclave, with 1.5 kg/cm pressure sterilizations 30 minutes:
Culture medium inoculated and cultural method are: when treating that substratum cools to 40 ℃ of following temperature, insert female bacterial classification or activation peace bottle dry freeze pipe bacterial classification by aseptic technique test tube slant substratum, secondary medium inserts inclined-plane or triangular flask bacterial classification, temperature is controlled at about 25-28 ℃, fermentation time generally was controlled at 48-72 hour, and microbial inoculum promptly can be used as the production microbial inoculum of bacterial manure after microscopy is qualified;
(5) making of AS4.891Steptomyces miroftauus streptomyces microflavus bacterial manure:
1. the making of slant strains: Zulkovsky starch 2 grams, lime carbonate KNO
31 gram, dipotassium hydrogen phosphate k
2HpO
40.05 gram, sodium-chlor NaCI0.05 gram, ferric sulfate FeS0
4.7H
2The O0.001 gram, agar 1.5 grams, 100 milliliters of distilled water, ph value 7.2-7.4;
2. dipotassium hydrogen phosphate k
2HpO
40.05 gram, sal epsom MgSO
4.7H
2The O0.05 gram, lime carbonate KNO
30.5 gram, soybean cake powder 20-40 part, loam 50-80 part, straw powder 25-50, calcium superphosphate 0.5-1.5 part, the peat composed of rotten mosses or plant ash or flyash 3-6 regulate about ph value 7-7.5 with unslaked lime; Medium sterilization, inoculation and cultural method are:
Medium sterilization: above slant medium, secondary microbial inoculum solid medium culture material are mixed, slant medium is packed in vitro, all the other secondary mediums are put into Cans or triangular flask or cloth bag, oral area or top are with cotton or multilayer gauze or kraft paper gag, substratum is put into or is inserted Autoclave, with 1.5 kg/cm pressure sterilizations 30 minutes;
Culture medium inoculated and cultural method are: when treating that substratum cools to 40 ℃ of following temperature, insert female bacterial classification or activation peace bottle dry freeze pipe bacterial classification by aseptic technique test tube slant substratum, secondary medium inserts inclined-plane or triangular flask bacterial classification, temperature is controlled at about 25-28 ℃, fermentation time generally was controlled at 48-72 hour, and microbial inoculum promptly can be used as the production microbial inoculum of bacterial manure after microscopy is qualified;
4. according to claim 1 or biological compounded granular fertilizer of 3 described a kind of Nuisanceless high-efficients and production method thereof, it is characterized in that the compatibility of above-mentioned bio-bacterial manure is:
AS1.824Azotbacter uinelandii Wei Nielande vinelandii and AS1.223 Bacillus megaterium lift Bacterium anthracoides and AS4.891Steptomyces miroftauus streptomyces microflavus; Or
AS1.824Azotbacter uine1andii Wei Nielande vinelandii and AS1.910 Bacillus muciLaginosus bacillusmusilaginosiengineering and AS1.867Pseudomonas fturescens Pseudomonas fluorescens;
5. according to claim 1 or biological compounded granular fertilizer of 3 or 4 described a kind of Nuisanceless high-efficients and production method thereof, it is characterized in that substratum, sterilization, inoculation, the fermentation of above-mentioned bio-bacterial manure is:
(1) substratum, sterilization, inoculation, the fermentation of AS1.824Azotbacter uinelandii Wei Nielande vinelandii and AS1.223 BaciLLus megaterium act Bacterium anthracoides and the bio-bacterial manure of AS4.891Steptomyces miroftauus streptomyces microflavus are:
Dipotassium hydrogen phosphate k
2HpO
40.01-0.05 part, potassium primary phosphate kh
2Po
40.01-0.02 part, sal epsom MgSO
4.7H
2O0.0-0.05 part, soya-bean cake 2-5 part, agricultural crop straw powder 3-8 part, wheat bran 2-5 part, calcium superphosphate 0.5-1.5 part, flyash or peat composed of rotten mosses 5-10 part, loam or organic 50-73 part, adjusting the ph value with lime is 7.5-8, and humidity is adjusted to about 30-40%, normal-pressure sterilization 2-3 hour or autoclaving 30 minutes, when treating that material cools to the 40 degree left and right sides, insert 2 grades of microbial inoculums, the access amount is 1-10%, insert after stirring in fermentation machine or fermentation frame or fermentation vat or the fermentation dish, shallow-layer (20 centimetres) or deep ventilation fermentation about control material temperature 30 degree, are not stirred, cultivated 3-5 days, and promptly can be high-quality bacterial manure;
(2) substratum, sterilization, inoculation, the fermentation of AS1.824Azotbacter uinelandii Wei Nielande vinelandii and AS1.910 BacillusmuciLaginosus bacillusmusilaginosiengineering and AS1.867Pseudomonas fturescens Pseudomonas fluorescens bacterial manure are:
Potassium primary phosphate kh
2Po
40.01-0.02 part, dipotassium hydrogen phosphate k
2HpO
40.04-0.02 gram, sal epsom Mgso
4.7h
2O0.01-0.02 part, 5 parts of soya-bean cakes, 8 parts in agricultural crop straw powder, 5 parts in wheat bran, 1 part of calcium superphosphate, 8 parts of the flyash or the peats composed of rotten mosses, 73 parts of loam or organic matters, adjusting the ph value with lime is 7.5-8, and humidity is adjusted to about 30-40%, normal-pressure sterilization 2-3 hour or autoclaving 30 minutes, when treating that material cools to the 40 degree left and right sides, insert 2 grades of microbial inoculums, the access amount is 1-10%, insert after stirring in fermentation machine or fermentation frame or fermentation vat or the fermentation dish, shallow-layer (20 centimetres) or deep ventilation fermentation about control material temperature 30 degree, are not stirred, cultivated 3-5 days, and promptly can be high-quality bacterial manure;
6. biological compounded granular fertilizer of a kind of Nuisanceless high-efficient according to claim 1 and production method thereof is characterized in that the preparation and the making of above-mentioned fertilizer:
Organic fertilizer comprises two classes:
(1) humic acid fertilizer is prepared and is made:
1. weathered coal or peat or peat composed of rotten mosses 60-100 part are ready to;
2. take by weighing 10-30 part dilute sulphuric acid proportion about 1.28, mix with an amount of water;
3. weathered coal or peat or the peat composed of rotten mosses are mixed with the mixture of dilute sulphuric acid and water, stir acidification 24-72 hour;
4. weathered coal or peat or the peat composed of rotten mosses and 15-30 part ground phosphate rock or the phosphate fertilizer mixing and stirring that acidification is crossed banked up and reacted 5-6 days, is humic-acid kind phosphate fertilizer;
(2) organic matter material fertilizer (comprising municipal wastes or animal excrement or fur offal or agricultural byproducts offal and various biscuit):
1. the residue of municipal wastes under residue after the magnetic separation stage treatment through banking up more than spontaneous fermentation 8-15 days, passes through 10-30 mesh sieve again, and filtrate is planted matter for organic and corruption, is good organic fertilizer after drying or drying;
2. add part grass meal or saw foam or weathered coal soil in the animal excrement, through spontaneous fermentation in 5-10 days or added three grades of bacteria fermentation 3-5 of biological organophosphorus bacterial fertilizer days, be good organic fertilizer after drying or drying;
3. the animal skin offal was pulverized 10-30 mesh sieve, added bio-azotobacter fertilizer material microbial inoculum and three grades of bacteria fermentation 4-6 of organophosphorus bacterial fertilizer days, was good organic protein fertilizer after drying or drying;
7. biological compounded granular fertilizer of a kind of Nuisanceless high-efficient according to claim 1 and production method thereof is characterized in that the preparation of above-mentioned inorganic fertilizer and manufacture craft are:
(1) amount of allocating into of inorganic raw material is: 30-60
Nitrogen, phosphorus, potassium content proportioning are: 3-8: 2-6: 1-5
1. urea content is 46.6%, adds (mass parts) to be: 5-19 part;
2. phosphorus content is 14%, and add-on (mass parts) is 20-50;
3. KCE content is 58%, and add-on (mass parts) is: 6-14 part;
(2) the trace element amount of allocating into (mass parts) is: 2-8 part, and its cooperation ratio is:
1. sal epsom 0.5-2.5,2. lime carbonate 0.5-2.5,3. boric acid 0.05-0.15,4. zinc sulfate 0.15-0.25,5. manganous sulfate 0.25-0.35,6. ammonium molybdate 0.005-0.015,7. copper sulfate 0.5-0.15,8. Xanthohumate 0.15-0.25,9. rare earth 0.04-0.095;
Make mixing granulation:
Elder generation is with fertilizer and humic acid phosphate fertilizer adds in the mixing machine or adding flyash; adding bio-bacterial manure again stirs; thereafter adding inorganics mixes; the composite fertilizer's batch mixing that mixes is arrived the tablets press granulation by conveyor delivery; air stream drying or oven drying at low temperature promptly become the complete first compounded granular fertilizer of high-effect organic biological.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101054568B (en) * | 2007-04-17 | 2010-05-26 | 周文彩 | Special biological organic fertilizer for tobacco, preparation and use method |
| CN101817698A (en) * | 2009-02-27 | 2010-09-01 | Atd废物处理系统有限公司 | System and method for producing organic based fertilizer and usable water from animal waste |
| CN102276367A (en) * | 2010-06-10 | 2011-12-14 | 王洪义 | Biological-organic-inorganic compound fertilizer and preparation method thereof |
| CN102424635A (en) * | 2011-09-19 | 2012-04-25 | 河北省微生物研究所 | Preparation method for composite continuous cropping resistant agent specially used for strawberries |
| CN102584370A (en) * | 2012-02-29 | 2012-07-18 | 王宝申 | Technology for preparing bio-fertilizer from garbage |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101054568B (en) * | 2007-04-17 | 2010-05-26 | 周文彩 | Special biological organic fertilizer for tobacco, preparation and use method |
| CN101817698A (en) * | 2009-02-27 | 2010-09-01 | Atd废物处理系统有限公司 | System and method for producing organic based fertilizer and usable water from animal waste |
| CN102276367A (en) * | 2010-06-10 | 2011-12-14 | 王洪义 | Biological-organic-inorganic compound fertilizer and preparation method thereof |
| CN102424635A (en) * | 2011-09-19 | 2012-04-25 | 河北省微生物研究所 | Preparation method for composite continuous cropping resistant agent specially used for strawberries |
| CN102424635B (en) * | 2011-09-19 | 2015-04-08 | 河北省微生物研究所 | Preparation method for composite continuous cropping resistant agent specially used for strawberries |
| CN102584370A (en) * | 2012-02-29 | 2012-07-18 | 王宝申 | Technology for preparing bio-fertilizer from garbage |
| CN104311363A (en) * | 2014-10-02 | 2015-01-28 | 青岛锐志农业技术服务有限公司 | Biochemical fertilizer for organic crops and production method thereof |
| CN104311363B (en) * | 2014-10-02 | 2017-03-08 | 青岛锐志农业技术服务有限公司 | A kind of organic agronomic crop biological fertilizer and its production method |
| CN104692968A (en) * | 2015-02-27 | 2015-06-10 | 中国热带农业科学院椰子研究所 | Special fertilizer for areca catechu with capability of swelling fruits as well as preparation method and application of special fertilizer |
| CN104692968B (en) * | 2015-02-27 | 2018-05-18 | 中国热带农业科学院椰子研究所 | A kind of betel nut strengthens fruit special fertilizer and its preparation method and application |
| CN109181709A (en) * | 2018-10-11 | 2019-01-11 | 江苏省水文水资源勘测局苏州分局 | A kind of soil conditioner and preparation method increasing soil permeability |
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