CN1313265A - Azotobacterium and potassium bacterium mixed fermentive culture medium for preparing composite bacterial fertilizer - Google Patents
Azotobacterium and potassium bacterium mixed fermentive culture medium for preparing composite bacterial fertilizer Download PDFInfo
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- CN1313265A CN1313265A CN 00103495 CN00103495A CN1313265A CN 1313265 A CN1313265 A CN 1313265A CN 00103495 CN00103495 CN 00103495 CN 00103495 A CN00103495 A CN 00103495A CN 1313265 A CN1313265 A CN 1313265A
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- potassium
- vinelandii
- vitamins
- potassium bacterium
- culture medium
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Abstract
A culture medium for culturing azotobacteria and potassium bacteria, which can be mixed and fermented to prepare composite bacteria fertilizer, contains soybean-sprout juice, cane sugar, starch, potassium salt, sodium salt, magnesium salt, calcium salt, trace elements and vitamines. Its advantages include stable pH value and osmotic pressure, and promoting growth and activity of bacteria.
Description
The present invention relates to a kind of vinelandii of compound bacterial fertilizer and substratum of potassium bacterium mixed fermentive produced.
The key of microbial fertilizer (also claim " bacterial manure ") quality is that institute contains amount of viable bacteria and quality, and the quality and quantity key of bacterium is fermention medium.Existing microbial fertilizer all contains one or more in vinelandii, phosphate solubilizing bacteria, the potassium solubilizing bacteria, generally adopts the separately fermentation separately of Chemical Composition synthetic medium, mixes then.Doing not only like this wastes time and energy, and the bacterium number is not high, mixes inhomogeneously, also is subjected to living contaminants easily, causes the fertilizer quality instability.
The invention provides a kind of substratum that forms by crude substance, inorganic salt and VITAMIN common combination, be fit to vinelandii and potassium bacterium mixed fermentive.Bacterial classification is provided by China Committee for Culture Collection of Microorganisms agricultural microorganism center, is respectively azotobacter chroococcum (Azotobacter chroococum), numbering ACCC10006; Bacillusmusilaginosiengineering (Bocillus mucilaginosus), numbering ACCC10013.The major ingredient of substratum has:
Soybean sprout juice 10% sucrose 0.5% starch 0.5%
Yeast powder 0.05% sal epsom 0.02% dipotassium hydrogen phosphate 0.1%
Sodium-chlor 0.02% lime carbonate 0.3% potassium felspar sand 0.1%
Liquid microelement 0.3% vitamins B
1The 3PPM vitamins B
123PPM
The present invention is through the production practice of repetition test and a year, and effect is remarkable.
1. the various nutrition reasonable ratio of this substratum can guarantee vinelandii and potassium bacterial growth needs, promptly can finish a fermentation period in 24-28 hour.
2. after this substratum only needs once to prepare burden, once sterilizes, can produce two kinds of bacterium, any material is no longer added in fermentation midway, can keep the stable of pH value in the whole fermentation process, have save time, the advantage of saving of labor, saving cost, avoided reinforced again midway easily by the phenomenon of living contaminants.
3. employing the present invention has rationally solved the equilibrium problem between the interior two kinds of bacteria growing desired nutritional materials of fermentor tank, has also solved the metastable problems of growing environment such as a jar interior potential of hydrogen, osmotic pressure and redox potential well.Every milliliter of viable count of nutrient solution can reach hundred million of 20-50, deposited half a year by peat composed of rotten mosses absorption after, every gram still contains hundred million of viable bacteria 2-3.
Below introduce the technology implementation example that the present invention provides:
Embodiment 1: the preparation of soybean sprout juice in the substratum
Take by weighing the soybean sprout that cultivates 1000 grams, add 1000 milliliters in water, boiled 30 minutes, cross leaching juice, moisturizing to 1000 milliliter promptly gets soybean sprout juice.
Embodiment 2: the preparation of liquid microelement in the substratum
Take by weighing Sodium orthomolybdate, boric acid, each 5 gram of iron trichloride, be dissolved into respectively in 1000 ml waters, be made into three kind of 0.5% liquid microelement.
Embodiment 3: fermentative medium formula
100 milliliters of soybean sprout juices, starch 5 grams, sucrose 5 grams, yeast powder 0.5 gram, sal epsom 0.2 gram, dipotassium hydrogen phosphate 1 gram, sodium-chlor 0.2 gram, lime carbonate 3 grams, potassium felspar sand 1 gram, 3 milliliters of liquid microelements (respectively 1 milliliter of 0.5% Sodium orthomolybdate, boric acid, iron trichloride), vitamins B
10.003 gram, vitamins B
120.003 gram.With above-mentioned composition mixing and water adding to 1000 milliliter, regulate pH value 7.0～7.4, the sterilization back fermention medium.
Claims (5)
1. vinelandii and a potassium bacterium mixed fermentive culture medium of producing compound bacterial fertilizer is characterized in that: be made up of soybean sprout juice 10%, sucrose 0.5%, starch 0.5%, yeast powder 0.05%, sal epsom 0.02%, dipotassium hydrogen phosphate 0.1%, sodium-chlor 0.02%, lime carbonate 0.3%, potassium felspar sand 0.1%, liquid microelement 0.3% and VITAMIN.
2. vinelandii according to claim 1 and potassium bacterium is characterized in that: vinelandii are azotobacter chroococcum (Azotobacter chroococum), numbering ACCC10006; The potassium bacterium is bacillusmusilaginosiengineering (Bacillusmucilaginosus), numbering ACCC10013; Two bacterial classifications are all provided by China Committee for Culture Collection of Microorganisms agricultural microorganism center.
3. mixed fermentation according to claim 1 is characterized in that vinelandii and potassium bacterium lump together, and ferment in same fermentor tank with above-mentioned substratum.
4. liquid microelement according to claim 1 is characterized in that: 0.5% Sodium orthomolybdate, 0.5% boric acid, 0.5% iron trichloride mix with volume.
5. VITAMIN according to claim 1 is characterized in that referring to vitamins B
1And vitamins B
12, the concentration in substratum all is 3PPM, that is: vitamins B
13PPM, vitamins B
123PPM.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB001034952A CN1178876C (en) | 2000-03-15 | 2000-03-15 | Azotobacterium and potassium bacterium mixed fermentive culture medium for preparing composite bacterial fertilizer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB001034952A CN1178876C (en) | 2000-03-15 | 2000-03-15 | Azotobacterium and potassium bacterium mixed fermentive culture medium for preparing composite bacterial fertilizer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1313265A true CN1313265A (en) | 2001-09-19 |
| CN1178876C CN1178876C (en) | 2004-12-08 |
Family
ID=4577028
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB001034952A Expired - Fee Related CN1178876C (en) | 2000-03-15 | 2000-03-15 | Azotobacterium and potassium bacterium mixed fermentive culture medium for preparing composite bacterial fertilizer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1178876C (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100372814C (en) * | 2005-06-20 | 2008-03-05 | 付锡臣 | Organic fertilizer prepared by using waste blackfungus culture medium and its preparation method and use |
| CN105272459A (en) * | 2015-10-23 | 2016-01-27 | 中盐安徽红四方肥业股份有限公司 | Production method of compound microorganism fertilizer |
-
2000
- 2000-03-15 CN CNB001034952A patent/CN1178876C/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100372814C (en) * | 2005-06-20 | 2008-03-05 | 付锡臣 | Organic fertilizer prepared by using waste blackfungus culture medium and its preparation method and use |
| CN105272459A (en) * | 2015-10-23 | 2016-01-27 | 中盐安徽红四方肥业股份有限公司 | Production method of compound microorganism fertilizer |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1178876C (en) | 2004-12-08 |
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