CN1297993A - Production process of liquid fungus strain for food and medicine - Google Patents
Production process of liquid fungus strain for food and medicine Download PDFInfo
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- CN1297993A CN1297993A CN00123385A CN00123385A CN1297993A CN 1297993 A CN1297993 A CN 1297993A CN 00123385 A CN00123385 A CN 00123385A CN 00123385 A CN00123385 A CN 00123385A CN 1297993 A CN1297993 A CN 1297993A
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Abstract
Waste stillage liquid from alcohol production is mixed with starch, cane sugar and yeast powder to compound culture medium and the first order liquid fungus strain is prepared. Then, the culture medium and the first order liquid fungus strain are used to produce liquid fungus strain through fermentation. The industrial production of liquid fungus strain can eliminate the pollution caused by the waste stillage liquid and lower the production cost of liquid fungus strain. The fungus strain has less passage times and high quality and is suitable for industrial production of edible fungus.
Description
The present invention is the production method of food, medicinal fungi liquid spawn, and more definite saying so utilizes the waste dregs liquid of producing alcohol to adopt the submerged fermentation technology to produce a kind of method of food, medicinal fungi liquid spawn.
Traditional production of hybrid seeds technology is to produce the secondary original seed to need directly to receive female kind the in inclined-plane on the pedigree seed culture medium; Producing three grades of cultivars is that original seed is directly received on the cultivar substratum.This production method production of hybrid seeds time is long, approximately needs 3 months, often misses best cultivation season because of production of hybrid seeds evening; Traditional in addition producing method for seed bacterial classification changes generation often, is difficult for guaranteeing strain quality; Old producing method for seed, inoculation are that solid spawn is received on the solid medium, and the face that contacts with each other between the bacterial classification is little, and it is slow to send out bacterium, and material feeding is slow, pollute easily.The production of hybrid seeds time of the present invention can shorten (10-15 days liquid spawn production cycle) greatly, and does not have the difference of secondary original seed and three grades of cultivars, changes the generation number and significantly reduces, and strain quality is good.Liquid spawn is beneficial to batch production production, and the success ratio height pollutes and lacks, and it is fast to send out bacterium, and the material feeding ability is strong.Both can commerial growing do the cultivar use, and also can do the secondary original seed and use.
The employing alcohol waste lees solution is a raw material, and production food, medicinal fungi liquid spawn one are the pollutions that has reduced grain distillery's waste discharge, makes the waste water of Alcohol Production producer obtain utilizing; The 2nd, the cost of producing bacterial classification reduces greatly.Have remarkable economic efficiency and social benefit.
One, the selection of slant strains is handled
1, bacterial classification is selected: adopt glossy ganoderma GL-903, excellent species such as black fungus 888, No. 4, mushroom, flat mushroom (Florida), Twospore Mushroom (summer mushroom 93).The bacterial classification source is Chaoyang City, Liaoning Province edible mushrooms institute and Jinxiang County, Shandong Province edible mushrooms institute.
2, the preparation of slant strains and processing: the slant strains substratum adopts PDA synthetic medium (potato 200 grams, white sugar 20 grams, agar 20 grams, potassium primary phosphate 3 grams, sal epsom 1.5 grams, water 1000ml), and cultivated 10-15 days down in 25-30 ℃ the inoculation back.Food, medicinal fungi bacterial classification adhesive ability on slant medium is very strong, if shovel directly places substratum after down, last shaking table is cultivated, the easy big bacterium piece of formation then, even have only a big bacterium fast, be unfavorable for further cultivation.So before inserting the primary inclined plane bacterial classification, will carry out fragmentation (using inoculating needle to carry out hand-crushed) to the primary inclined plane bacterial classification, after the break process, bacterial classification can float on fluid surface, the bacterial classification good dispersity after inserting new liquid nutrient medium, it is many to send out bacterium point, and bacterial classification amount and bacterium ball density are increased.
Two, level liquid strain preparation
1, the preparation of substratum: corn alcohol waste dregs liquid filtered liquid 96.8-100% (6-8 layer filtered through gauze) adds 0-2% starch, 0-1% sucrose, and 0-0.2% yeast powder, PH are 5.5-6.5.
2, level liquid strain preparation: in the capitate bottle of 250ml, add the 70-90ml substratum, sterilized 30 minutes for 15 pounds, insert the slant strains fragment when being cooled to 30 ℃, inoculum size is 10%, under 28 ℃, left standstill 24 hours earlier, put 180rpm on the rotary shaking table again, cultivated 48-72 hour for 28 ℃.
Three, the preparation of liquid spawn
1, the preparation of substratum: corn alcohol waste dregs liquid 96-100% (centrifugal 20 minutes, the different throw out that goes) adds 0-2% sucrose, 0-2% Zulkovsky starch, PH5.5-6.5.
2, liquid spawn preparation: corn alcohol waste dregs liquid substratum is placed fermentor tank, after the sterilization cooling, inoculation level liquid bacterial classification 6-10%.Ventilation stirred pot culture condition 0-12 hour, stirring velocity are 80rpm, and ventilation is 0.5: 1vvm; 12-72 hour, stirring velocity was 120rpm, and ventilation is 0.5: 1vvm; 72-96 hour, stirring velocity was 180rpm, and ventilation is 0.5: 1vvm.
3, the aftertreatment of liquid spawn: cultured liquid spawn was staticly settled 24 hours, take the nutrient solution on mycelium top away, replenish the distilled water of equivalent then.
4, packing: canned under aseptic condition, to put under the 4-28 ℃ of condition and preserve, the quality guaranteed period is 6 months.In storage and transport, prevent to surpass 30 ℃ of high temperature, before the use inoculation, shake up the back and use.
Four, description of drawings
Fig. 1 is a process flow sheet of the present invention.
Five, the present invention has following advantage
1, alcohol waste lees solution is fully used.
2, produce bacterial classification with the alcohol waste lees solution batch production.
3, solved the environmental pollution of alcohol waste lees solution.
Six, the domestic and international situation of Slops treatment
It is that various countries produce the main production method that alcohol adopts that fermentation method is produced alcohol, gives birth to Produce a large amount of waste liquids in the product, one ton of alcohol of every production will discharge 10-15 ton waste liquid. Extremely Nineteen ninety ends, and the alcohol effluent amount of the annual discharging of China reaches more than 1,500 ten thousand tons, adds again The discharge capacity of upper liquid distillery (workshop) is estimated can reach 4,000 ten thousand tons every year. This is not Only wasted a large amount of grains, simultaneously also severe contamination environment, useless such as cereal materials COD value in the liquid is up to 40000-50000mg/l, and BOD5 reaches 20000-30000mg/l. The numerical value of molasses alcohol waste liquid is just higher, general COD value 50000-80000mg/l, B0D530000-50000mg/l。
Contain abundant nutriment in the alcohol effluent, in the grain alcohol waste liquid, contain Protein, cellulose, poly-pentose, ash content, fat, caramel, abundant B family are arranged Vitamin and auxin; Remove in the molasses alcohol waste liquid and contain abundant carbon source, nitrogenous source and phosphorus Outside the source, be rich in sylvite, calcium salt etc. The chief component of alcohol effluent is as shown in table 1.
The composition of table 1 alcohol effluent
| Component | The corn alcohol waste liquid | Molasses alcohol waste liquid |
| BOD(mg/l) | 15000-25000 | 50000-60000 |
| Solid content (%) | 7.5 | 8.5 |
| Carbohydrate (%) | 0.5 | 0.9 |
| Protein (%) | 2.3 | 0.8 |
| Ash content (%) | 1.5 | 2.5 |
| Vitamin | More | More |
Fully utilize the various situations of alcohol effluent both at home and abroad:
1. produce (SCP) feed
2. production full price dry feed (DDGS)
3. biogas fermentation.
Embodiment
Level liquid spawn culture based formulas (precentagewise)
1, corn alcohol waste dregs liquid filtered liquid (6-8 layer filtered through gauze), 100, PH6.5.
2, corn alcohol waste dregs liquid filtered liquid (6-8 layer filtered through gauze), 98.4, starch 1, sucrose 0.5, yeast powder 0.1, PH6.5.
3, corn alcohol waste dregs liquid filtered liquid (6-8 layer filtered through gauze), 96.8, starch 2, sucrose 1, yeast powder 0.2, PH6.5.
Liquid spawn culture medium prescription (precentagewise)
1, corn alcohol waste dregs liquid (centrifugal 20 minutes, the different throw out that goes) 100, PH6.5.
2, corn alcohol waste dregs liquid (centrifugal 20 minutes, the different throw out that goes) 98, sucrose 1, starch 1, PH6.5.
3, corn alcohol waste dregs liquid (centrifugal 20 minutes, the different throw out that goes) 96, sucrose 2, starch 2, PH6.5.
Claims (2)
1, produce the method for food, medicinal fungi liquid spawn, it is characterized in that:
1. level liquid strain preparation
The preparation of a, substratum: alcohol waste lees solution filtrate, pH value 5.5-6.5,
The preparation of b, level liquid bacterial classification: in 250 milliliters capitate bottle, add 70-90 milliliter substratum, sterilized 30 minutes for 15 pounds, insert the slant strains fragment when being cooled to 30 ℃, inoculum size is 10%, under 28 ℃, left standstill 24 hours earlier, put on the rotary shaking table 180 rev/mins again, cultivated 48-72 hour for 28 ℃
2. the preparation of liquid spawn
The preparation of a, substratum: the alcohol waste lees solution disgorging, pH value 5.5-6.5,
The preparation of b, liquid spawn: the alcohol waste lees liquid culture medium is placed fermentor tank, after the sterilization cooling, inoculation level liquid bacterial classification 6-10%, ventilation stirred pot culture condition 0-12 hour, 80 rev/mins of stirring velocitys, ventilation is 0.5: 1vvm; 12-72 hour, stirring velocity was 120 rev/mins, and ventilation is 0.5: 1vvm; 72-96 hour, stirring velocity was 180 rev/mins, and ventilation is 0.5: 1vvm,
The aftertreatment of c, liquid spawn: cultured liquid spawn was staticly settled 24 hours, take the nutrient solution on mycelium top away, replenish the distilled water of equivalent then,
D, packing: canned under aseptic condition, to put under the 4-28 ℃ of condition and preserve, the quality guaranteed period is 6 months, prevents to surpass 30 ℃ of high temperature in storage and transport, before the use inoculation, shakes up the back and uses.
2, according to the said method of claim 1, it is characterized in that:
A, preparation level liquid culture of strains base are to get corn alcohol waste dregs liquid filtrate 97-100%, starch 0-2%, and sucrose 0-1%, yeast powder 0-0.2%, PH5.5-6.5,
The substratum of b, preparation liquid spawn is the corn alcohol waste dregs liquid 96-100% that gets disgorging, sucrose 0-2%, Zulkovsky starch 0-2%, PH5.5-6.5.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN00123385A CN1297993A (en) | 2000-12-08 | 2000-12-08 | Production process of liquid fungus strain for food and medicine |
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| Application Number | Priority Date | Filing Date | Title |
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| CN00123385A CN1297993A (en) | 2000-12-08 | 2000-12-08 | Production process of liquid fungus strain for food and medicine |
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| CN1297993A true CN1297993A (en) | 2001-06-06 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8481295B2 (en) | 2007-06-20 | 2013-07-09 | Johannes van Leeuwen | Fungi cultivation on alcohol fermentation stillage for useful products and energy savings |
| CN103404366A (en) * | 2013-07-01 | 2013-11-27 | 鲁东大学 | Black abalone mushroom liquid culture preparing and high-yield cultivation method |
| US9079786B2 (en) | 2006-06-20 | 2015-07-14 | Johannes van Leeuwen | Purification of thin stillage from dry-grind corn milling with fungi |
| CN107641601A (en) * | 2017-10-20 | 2018-01-30 | 翔天农业开发集团股份有限公司 | A kind of liquid fermentation strain domestication method |
| CN108546174A (en) * | 2018-07-11 | 2018-09-18 | 广东泓睿科技有限公司 | A kind of selenium-enriched edible mushroom culture medium and preparation method thereof |
-
2000
- 2000-12-08 CN CN00123385A patent/CN1297993A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9079786B2 (en) | 2006-06-20 | 2015-07-14 | Johannes van Leeuwen | Purification of thin stillage from dry-grind corn milling with fungi |
| US8481295B2 (en) | 2007-06-20 | 2013-07-09 | Johannes van Leeuwen | Fungi cultivation on alcohol fermentation stillage for useful products and energy savings |
| CN103404366A (en) * | 2013-07-01 | 2013-11-27 | 鲁东大学 | Black abalone mushroom liquid culture preparing and high-yield cultivation method |
| CN107641601A (en) * | 2017-10-20 | 2018-01-30 | 翔天农业开发集团股份有限公司 | A kind of liquid fermentation strain domestication method |
| CN108546174A (en) * | 2018-07-11 | 2018-09-18 | 广东泓睿科技有限公司 | A kind of selenium-enriched edible mushroom culture medium and preparation method thereof |
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