CN121294127A - A fermentation device based on industrial automation - Google Patents
A fermentation device based on industrial automationInfo
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- CN121294127A CN121294127A CN202511565378.0A CN202511565378A CN121294127A CN 121294127 A CN121294127 A CN 121294127A CN 202511565378 A CN202511565378 A CN 202511565378A CN 121294127 A CN121294127 A CN 121294127A
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Abstract
The invention relates to the technical field of fermentation equipment, in particular to a fermentation device based on industrial automation, which adopts a sectional tank design, realizes linear expansion of fermentation capacity by increasing and decreasing the number of middle standard tanks, solves the limitation of fixed volume of a traditional fermentation tank, integrates a ventilation defoaming unit, efficiently supplies oxygen through a tank bottom annular gas distributor, automatically adjusts tank top pressure based on a foam height signal to realize physical defoaming, effectively reduces the use of chemical defoaming agents, and also integrates a stirring unit, a temperature control unit, a process detection unit and a gas separation unit which are uniformly coordinated by a process control unit, thereby realizing full-course automatic closed-loop control from air intake, stirring, temperature control to defoaming and tail gas treatment. The invention solves the problems of fixed volume, large defoaming pollution and poor system cooperativity of the traditional fermentation tank, and remarkably improves the stability, efficiency and product quality of the fermentation process.
Description
Technical Field
The invention relates to the technical field of fermentation equipment, in particular to a fermentation device based on industrial automation.
Background
Industrial fermentation is a key step of biotechnology achievement industrialization, and is widely applied to the fields of pharmacy, food, chemical industry, energy sources and the like. Along with the development of synthetic biology and metabolic engineering, engineering strains capable of efficiently expressing target products are continuously emerging, and higher requirements are put on a fermentation device for carrying the reactions of the engineering strains. An ideal fermentation device should be able to provide a completely controlled, stable and reproducible biological environment to support optimal growth of the bacteria and efficient synthesis of metabolites.
CN104911100a discloses a modularized solid biological raw material continuous fermentation device, and from the structural description, the modularized core is that multiple layers of fermentation chambers are connected in series, each layer of fermentation chamber is formed by arranging multiple box units with the same structure along the transverse direction, and the fermentation chambers are connected in series through a conveying device, so that continuous feeding and discharging of solid materials and serialization of fermentation processes are realized. This structure is essentially a cluster of multiple fixed volume reactors and is not intended to change the effective volume of a single reactor. The structure aims at the specific process of solid state fermentation, and cannot be suitable for liquid submerged fermentation of the main stream.
In terms of fermentation process control, oxygen supply and defoaming are two interrelated and critical challenges. In the aerobic fermentation process, sufficient dissolved oxygen is required to be provided for the thalli by stirring and ventilation. The traditional ventilation system has uneven bubble size, and the oxygen transfer efficiency is required to be improved. Meanwhile, a large amount of foam is easy to generate due to intense stirring and ventilation, and excessive foam can cause liquid escape, increase the risk of bacteria contamination and cause loss of precious products. At present, a mode of adding a chemical defoaming agent is generally adopted in the industry for intervention, but the addition of the defoaming agent can produce an unknown inhibition effect on the growth and metabolism of thalli, and foreign impurities can be introduced, so that the difficulty and cost of subsequent separation and purification are increased. Although there is a physical defoaming technique (such as a mechanical defoaming paddle), the effect is often insufficient when facing severe bubbles, and precise automatic control is difficult to realize.
In addition, the degree of automation of the existing fermentation device is mainly independent control of single parameters (such as temperature and pH), efficient collaborative linkage is lacked among systems (such as air inlet, stirring and defoaming), and complex dynamic changes in the fermentation process are difficult to deal with. Volatile active ingredients in the fermentation tail gas are also often directly discharged, so that not only is the resource wasted, but also the environmental problem is possibly caused.
Therefore, the fermentation tank has the following problems that in terms of structural flexibility, the existing modularized scheme cannot meet the requirement of linear and continuous adjustment of the tank body volume, in terms of process control, the existing defoaming technology is mainly based on a chemical method, although the existing defoaming technology has individual physical defoaming devices, the problems of insufficient control precision, delayed response and the like generally exist, effective coordination with an oxygen supply system cannot be formed, and in terms of system integration level, each control unit often operates independently and lacks a unified cooperative control strategy, so that the energy efficiency and the product yield of the whole fermentation system are difficult to further improve. Therefore, the development of a novel fermentation device capable of simultaneously solving the problems of flexible volume adjustment, intelligent defoaming and oxygen supply coordination and overall process integrated optimization control is urgently needed in the field
Disclosure of Invention
Therefore, the invention provides a fermentation device based on industrial automation, which is used for solving the problems of poor equipment flexibility, large pollution in defoaming mode and weak system cooperativity in the prior art.
To achieve the above object, the present invention provides an industrial automation-based fermentation apparatus comprising:
The sectional tank body comprises a cylindrical main body, wherein the cylindrical main body is formed by detachably and hermetically connecting a seal head tank body, a plurality of middle standard tank bodies and a lower tank body from top to bottom, and the total capacity of the sectional tank body is adjusted by increasing or decreasing the number of the middle standard tank bodies;
The stirring unit is arranged on the end socket tank body and comprises a segmented paddle shaft driven by a differential driving assembly, a shaft fixing device fixed at the bottom of the tank and a plurality of layers of paddles arranged on the paddle shaft;
The ventilation defoaming unit is connected with the sectional tank body, is used for adjusting the gas pressure in the sectional tank body to physically defoam the fermentation environment by monitoring the gas pressure in the sectional tank body, and is connected with the air compressor to start the compressor for ventilation;
The process detection unit is arranged in the sectional tank body and used for monitoring the temperature, the pH value, the dissolved oxygen amount of the fermentation liquid and the foam height in the tank in real time;
the temperature control unit comprises a jacket arranged outside the tank body and/or a coil pipe arranged in the tank body and is used for adjusting the temperature in the sectional tank body;
The gas separation unit is connected with the ventilation defoaming unit and is used for separating and recycling the effective components in the fermentation tail gas;
The process control unit is respectively connected with the stirring unit, the ventilation defoaming unit, the process detection unit, the temperature control unit and the gas separation unit in a signal manner, and is used for receiving real-time data of the process detection unit, sending control instructions to each execution unit based on a preset fermentation process curve, and realizing automatic closed-loop control of the fermentation process, wherein the automatic closed-loop control comprises the step of adjusting the tank top pressure regulating valve according to a foam height signal so as to execute physical defoaming operation. Further, the seal head tank body comprises a seal head and a tank body, the seal head is hinged to the tank body, and the tank body is provided with a feed inlet and an exhaust port; the upper edge of the outer side of the seal head is provided with a bulge, the lower end of the lower tank body is provided with a butt joint part matched with the bulge, and one segmented tank body is fastened with the butt joint part of the other segmented tank body through a clamp or a bolt so as to realize sealing connection;
the lower edge of the seal head tank body is provided with a clamping groove structure which is used for being matched with a flange at the upper end of the middle standard tank body to realize sealing connection, and both ends of the middle standard tank body are provided with the flange and the clamping groove structure;
The lower end of the lower tank body is provided with a discharge port and an air inlet, and the edge of the lower end of the lower tank body is provided with a butt joint part and a shaft fixing device which is connected with the lower tank body in a penetrating way;
The stirring unit comprises a differential driving assembly, two or more sets of segmented tank bodies with the same specification are arranged in series to form a series fermentation system, wherein the differential driving assembly is connected at the central axis of the sealing head in a penetrating way;
In the series fermentation system, the axial butt joint and power transmission of the shaft fixing device of one sectional type tank body and the differential driving assembly of the other sectional type tank body are realized through a coupling structure, and the process control unit respectively and independently controls the operation parameters of each sectional type tank body, so that the multi-tank collaborative large-scale fermentation production is realized.
The stirring unit comprises a plurality of layers of paddles symmetrically arranged, each layer of paddles is connected through a segmented paddle shaft, the paddle shafts are fixed through a shaft coupling structure, the upper ends of the paddle shafts are connected with the output end of the differential driving assembly, the lower ends of the paddle shafts are connected with the shaft fixing device, the shaft fixing device is fixed at the center position of the bottom end in the lower tank body, and the differential driving assembly adjusts the rotating speed according to the fermentation process to realize multistage variable speed stirring.
Further, the differential drive assembly comprises a main drive motor and an auxiliary speed change mechanism, and the differential drive assembly protrudes out of the plane of the seal head and is lower than the height of the protruding part and is connected with the other segmented tank body lower tank body shaft fixing device to form reinforcing connection.
Further, in the ventilation defoaming unit, an air outlet pipeline of the air compressor is sequentially connected with a pressure regulating valve, a pressure gauge and an annular gas distributor at the bottom of the tank, and micropores are uniformly distributed on the surface of the annular gas distributor;
The tank top pressure regulating valve is arranged on an exhaust port pipeline of the end socket tank body, the pressure regulating valve is linked with the progress detecting unit, and when the foam height is detected to reach a preset value, the progress control unit controls the tank top pressure regulating valve to lift the pressure in the tank, so that physical defoaming is realized.
Further, a gas-liquid separation film is arranged on the exhaust port pipeline in front of the tank top pressure regulating valve and used for blocking liquid foam and microorganisms from escaping along with gas, so that the loss of bacteria and feed liquid is prevented.
Further, the process detection unit comprises a pH sensor, an oxygen dissolving electrode, a temperature probe, a liquid level sensor and a foam sensor, so that the pH value, the concentration of the dissolved oxygen, the temperature, the liquid level and the foam height in the fermentation process are monitored in real time, and data are transmitted to the process control unit.
Further, the temperature control unit comprises a coil heating and cooling device and an outer jacket structure, wherein a coil of the coil heating and cooling device is arranged in a region near the bottom of the inner wall of the tank body, the outer jacket surrounds the outside of the tank body, and the coil of the coil heating and cooling device and the outer jacket are respectively connected with a circulating medium to realize temperature rise and reduction.
Further, the gas separation unit comprises an exhaust port positioned at the upper part of the seal head tank body, and the exhaust port is connected with a condensation separation mechanism and is used for condensing and recycling volatile effective components in exhaust gas and water vapor.
Further, the process control unit comprises a PLC controller, a man-machine interaction interface and a data communication module;
the PLC records various parameters of the fermentation process in real time, including temperature, pH value, dissolved oxygen, stirring speed, pressure in the tank, foam height, air inlet flow and fermentation time, stores the data in a database, and analyzes the historical data to optimize fermentation process parameters;
the man-machine interaction interface provides functions of real-time data visualization, parameter setting, alarm prompting and process recipe management;
the data communication module supports Modbus, PROFIBUS, ethernet and wireless communication protocols, and realizes data integration with a factory-level monitoring system;
The process control unit is preset with an intelligent control algorithm, and can dynamically adjust stirring rotation speed, air inflow, tank pressure and temperature parameters according to real-time fermentation data, so as to realize predictive control and self-adaptive optimization of the fermentation process.
Compared with the prior art, the invention has the beneficial effects that,
The invention is formed by connecting the end socket tank body, the middle standard tank bodies and the lower tank body through a detachable sealing structure through sectional type modularized tank body design. The total capacity of the sectional type tank bodies can be adjusted by increasing or decreasing the number of the middle standard tank bodies, so that the requirements of different production scales are met, the equipment utilization rate and the production flexibility are obviously improved, and the equipment investment cost is reduced.
Furthermore, the ventilation system and the physical defoaming function are integrated in the same unit, the annular gas distributor at the bottom of the tank is adopted to realize high-efficiency oxygen supply, and meanwhile, the pressure regulating valve at the top of the tank is adopted to realize physical defoaming. When the foam height reaches a preset value, the process control unit automatically adjusts the pressure regulating valve, improves the pressure in the tank to realize non-contact physical defoaming, thoroughly gets rid of dependence on a chemical defoaming agent, and avoids inhibition of thallus metabolism and product pollution.
Furthermore, the process control unit is used for uniformly coordinating the stirring unit, the ventilation defoaming unit, the process detection unit, the temperature control unit and the gas separation unit, and the closed-loop linkage control of multiple parameters and multiple variables is realized based on fermentation parameters monitored in real time. The system can dynamically respond to complex changes of the fermentation process, remarkably improve the accuracy and stability of process control, and ensure the consistency of product quality.
Furthermore, the invention realizes the mechanical integration and power transmission of a plurality of segmented tanks through the butt joint structure of the shaft fixing device and the differential driving assembly, thereby forming an expandable series fermentation system. The system keeps each tank body to independently operate while using the same set of gas separation unit and process control unit, thereby improving the space utilization rate of equipment and realizing flexible configuration of large-scale production.
Furthermore, the invention realizes rapid and accurate temperature regulation by adopting a coil pipe type heating and cooling device and an outer jacket structure to respectively control through an independent temperature control unit. The differential driving component can adjust the rotating speed according to the fermentation process, realize multistage variable-speed stirring, and meet the process requirements of different strains and fermentation stages.
Further, the process control unit monitors key parameters of the pH value, the dissolved oxygen concentration, the temperature, the liquid level and the foam height in real time through the process detection unit, and combines a preset intelligent control algorithm, so that the process control unit can dynamically optimize fermentation process parameters based on real-time data and historical data analysis. By continuous learning and parameter self-tuning, the system can continuously improve fermentation efficiency and product quality, and realize continuous improvement and optimization of the process.
Drawings
FIG. 1 is a schematic view of a single tank and modular connection structure of a fermentation device based on industrial automation in accordance with an embodiment of the present invention;
FIG. 2 is a schematic diagram of a series fermentation system according to an embodiment of the present invention;
FIG. 3 is a schematic structural view of a stirring unit according to an embodiment of the present invention;
FIG. 4 is a schematic diagram of the connection of the axle holder to the differential drive assembly according to an embodiment of the present invention;
in the figure, the device comprises a 1-end socket tank body, a 2-middle standard tank body, a 3-lower tank body, a 4-protruding part, a 5-butt joint part, a 6-clamping groove structure, a 7-flange, an 8-differential driving assembly, a 9-shaft fixing device, a 10-feeding port, a 11-discharging port, a 12-outer jacket, a 13-air inlet, a 14-air outlet, a 15-first tank body, a 16-second tank body, a 17-segmented propeller shaft, a 18-first propeller shaft, a 19-second propeller shaft and 20-propeller blades.
Detailed Description
The invention will be further described with reference to examples for the purpose of making the objects and advantages of the invention more apparent, it being understood that the specific examples described herein are given by way of illustration only and are not intended to be limiting.
Preferred embodiments of the present invention are described below with reference to the accompanying drawings. It should be understood by those skilled in the art that these embodiments are merely for explaining the technical principles of the present invention, and are not intended to limit the scope of the present invention.
It should be noted that, in the description of the present invention, terms such as "upper," "lower," "left," "right," "inner," "outer," and the like indicate directions or positional relationships based on the directions or positional relationships shown in the drawings, which are merely for convenience of description, and do not indicate or imply that the apparatus or elements must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present invention.
In addition, it should be noted that, in the description of the present invention, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, integrally connected, mechanically connected, electrically connected, directly connected, indirectly connected through an intermediate medium, or in communication between two elements. The specific meaning of the above terms in the present invention can be understood by those skilled in the art according to the specific circumstances.
The invention provides a fermentation device based on industrial automation, which comprises:
The sectional tank body comprises a cylindrical main body, wherein the cylindrical main body consists of a seal head tank body 1, a plurality of middle standard tank bodies 2 and a lower tank body 3 from top to bottom through a detachable sealing structure, and the total capacity of the sectional tank body is adjusted by increasing or decreasing the number of the middle standard tank bodies 2;
the stirring unit is arranged on the end socket tank body 1 and comprises a segmented propeller shaft 17 driven by the differential driving assembly 8, a shaft fixing device 9 fixed on the bottom of the tank and a plurality of layers of paddles 20 arranged on the segmented propeller shaft 17;
the ventilation defoaming unit is connected with the sectional tank body, realizes physical defoaming by monitoring and adjusting the pressure of gas in the tank, and is connected with the air compressor for ventilation;
The process detection unit is arranged in the sectional tank body and is used for monitoring the pH value, the concentration of dissolved oxygen, the temperature, the liquid level and the foam height in the tank in real time;
the temperature control unit comprises a jacket 12 arranged outside the tank body and/or a coil pipe of the coil pipe type heating and cooling device arranged in the tank body and is used for regulating and controlling the fermentation temperature;
the gas separation unit is connected with the ventilation defoaming unit and is used for separating and recycling the effective components in the fermentation tail gas;
the process control unit is respectively connected with the stirring unit, the ventilation defoaming unit, the process detection unit, the temperature control unit and the gas separation unit in a signal manner, and realizes automatic closed-loop control of the fermentation process based on a preset fermentation process curve.
Referring to fig. 1 and 3, fig. 1 is a schematic diagram of a single tank and a modular connection structure of a fermentation device based on industrial automation according to an embodiment of the present invention, and fig. 3 is a schematic diagram of a stirring unit according to an embodiment of the present invention;
The sectional tank body shown in the figure realizes rapid assembly and sealing among the tank body sections through a standardized flange interface, and is convenient for transportation and field installation. The differential driving assembly 8 can adjust and stir the rotation speed ratio of the segmented paddle shaft 17, so that the paddles 20 of different layers adapt to the mixing requirements of each fermentation stage, and the mass transfer efficiency is improved.
Specifically, the sectional tank body adopts the modularized design, the end socket tank body 1 is provided with the feed inlet 10 and the exhaust port 14, the outer upper edge of the end socket is provided with the protruding part 4, and the protruding part 4 is in sealing connection with the butt joint part 5 of the lower tank body 3 through clamping rings or bolt fastening. The lower edge of the end socket tank body is provided with the clamping groove structure 6 which is matched with the 7 flange at the upper end of the middle standard tank body, so that the sectional tank bodies can be quickly disassembled and sealed. The process control unit integrates the Internet of things module, supports remote monitoring and multi-tank cooperative regulation and control, and ensures process consistency among batches. The whole device has compact structure and is suitable for large-scale industrial continuous production.
Specifically, the stirring unit is designed by adopting the sectional type paddle shaft 17, each section of the sectional type paddle shaft 17 is connected through the coupling structure, a plurality of layers of paddles 20 are arranged on the sectional type paddle shaft 17, and the differential driving assembly 8 can adjust the rotating speed according to the fermentation process so as to realize multistage variable-speed stirring. The shaft fixing device 9 is fixed at the center of the bottom of the lower tank body 3, and ensures the stable operation of the stirring shaft.
Referring to fig. 2 and 4, fig. 2 is a schematic structural diagram of a serial fermentation system according to an embodiment of the present invention, and fig. 4 is a schematic connecting diagram of a shaft fixer and a differential driving assembly according to an embodiment of the present invention;
Specifically, the series fermentation system consists of two or more sets of segmented tank bodies with the same specification. The segmented tank bodies are mutually matched with the protruding parts 4 through the butt joint parts 5 to realize mechanical integration, and the shaft fixing device 9 and the differential driving assembly 8 of the adjacent segmented tank bodies realize power transmission through a coupling structure. The serial system can use the same set of gas separation unit and process control unit to realize the multi-tank collaborative large-scale fermentation production.
In this embodiment, the first tank body 15 and the second tank body 16 are set, the two are in nested butt joint with the butt joint part 5 of the lower tank body 3 of the first tank body 15 through the boss part 4 of the upper end socket tank body 1 of the second tank body 16, and the tight connection between the two tank bodies is realized through a sealing ring and a fastening bolt, meanwhile, the differential driving assembly 8 of the first tank body 15 and the bottom of the second tank body 16 form driving linkage with the shaft fixing device 9, and are connected in series, so that the two tanks share the same process control unit and the gas separation unit, synchronous operation and efficient utilization of resources are realized, and energy consumption and equipment occupation are remarkably reduced. It should be understood by those skilled in the art that the segmented tank can be flexibly expanded according to actual capacity requirements, and seamless connection is realized among a plurality of tanks through a unified interface standard.
Specifically, the ventilation defoaming unit comprises an air compressor, a pressure regulating valve, a pressure gauge and an annular gas distributor. The air is pressurized by a compressor, then flows are regulated and controlled by the pressure regulating valve, and enters the fermentation liquor in the form of tiny bubbles through the annular gas distributor. The tank top is provided with the pressure regulating valve, and when the foam height is detected to reach a preset value, the process control unit regulates the pressure of the tank top through the pressure regulating valve to realize physical defoaming.
Specifically, the process detection unit comprises a pH sensor, an oxygen dissolving electrode, a temperature probe, a liquid level sensor and a foam sensor, wherein each sensor is led out through a waterproof electric connector through a sterile interface arranged on the wall surface of the sealing head or the tank body, and finally is connected to the process control unit in a summarizing way. The pH sensor is preferably arranged on the upper tank body so as to monitor the pH value change of the fermentation liquor in real time, and the dissolved oxygen sensor is arranged on the side wall of the middle-lower tank body so as to ensure the accurate capture of the concentration of the dissolved oxygen. The temperature sensors are distributed along the axial direction of the tank body, so that synchronous monitoring of different depth temperatures of the fermentation liquid is realized, a temperature measurement blind area is eliminated, and the distribution of the temperature field in the tank is truly reflected.
Specifically, the temperature control unit adopts a double design of a coil pipe type heating and cooling device and an outer jacket 12 structure, a coil pipe is arranged in a region near the bottom of the inner wall of the tank body, the outer jacket 12 surrounds the outside of the tank body, and the coil pipe and the outer jacket are respectively connected with a circulating medium to realize rapid temperature rise and drop and can be independently controlled.
Specifically, the gas separation unit is connected to the exhaust port 14 pipeline, and the volatile effective components in the exhaust gas and the water vapor are condensed and recovered through the efficient condensation separation mechanism, so that the resource utilization rate is improved.
Specifically, the process control unit adopts an industrial PLC controller as a core processing unit, and an intelligent control system with self-learning capability is constructed by integrating intelligent algorithms such as fuzzy PID control, model predictive control, a self-adaptive neural network and the like. The unit is provided with a high-resolution man-machine interaction interface and an industrial Ethernet communication module, key process parameters such as temperature, pH value, dissolved oxygen concentration, foam height and the like are collected and recorded in real time, and a complete fermentation process database is established. Based on historical data analysis and machine learning technology, the system can automatically identify the association rule of process parameters and product quality, continuous optimization of the fermentation process is realized through parameter self-tuning, and consistency of product quality and repeatability of a production process are remarkably improved.
Through the technical scheme, the flexible adjustment of the volume of the fermentation device, the intelligent control of physical defoaming, the cooperative operation of multiple systems and the efficient utilization of resources are realized, and the problems of poor flexibility of equipment, large pollution in a defoaming mode and weak system cooperativity in the prior art are effectively solved.
Thus far, the technical solution of the present invention has been described in connection with the preferred embodiments shown in the drawings, but it is easily understood by those skilled in the art that the scope of protection of the present invention is not limited to these specific embodiments. Equivalent modifications and substitutions for related technical features may be made by those skilled in the art without departing from the principles of the present invention, and such modifications and substitutions will be within the scope of the present invention.
Claims (10)
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| CN117946851A (en) * | 2024-03-07 | 2024-04-30 | 大连大学 | Fermentation tank stirring device |
| WO2025130986A1 (en) * | 2023-12-20 | 2025-06-26 | 中国科学院天津工业生物技术研究所 | Airlift bioreactor |
| CN120366031A (en) * | 2025-04-22 | 2025-07-25 | 西北农林科技大学 | Potato starch processing waste residue fermentation device and method |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2025130986A1 (en) * | 2023-12-20 | 2025-06-26 | 中国科学院天津工业生物技术研究所 | Airlift bioreactor |
| CN117946851A (en) * | 2024-03-07 | 2024-04-30 | 大连大学 | Fermentation tank stirring device |
| CN120366031A (en) * | 2025-04-22 | 2025-07-25 | 西北农林科技大学 | Potato starch processing waste residue fermentation device and method |
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