CN120366031A - Potato starch processing waste residue fermentation device and method - Google Patents

Potato starch processing waste residue fermentation device and method

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Publication number
CN120366031A
CN120366031A CN202510508999.9A CN202510508999A CN120366031A CN 120366031 A CN120366031 A CN 120366031A CN 202510508999 A CN202510508999 A CN 202510508999A CN 120366031 A CN120366031 A CN 120366031A
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fermentation
tank body
sterile
air
potato starch
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Inventor
钱�勋
席娇
谷洁
闫雪军
孙薇
王小娟
宋籽霖
胡婷
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Northwest A&F University
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Northwest A&F University
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Priority to CN202510508999.9A priority Critical patent/CN120366031A/en
Publication of CN120366031A publication Critical patent/CN120366031A/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure

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  • Engineering & Computer Science (AREA)
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  • Molecular Biology (AREA)
  • Processing Of Solid Wastes (AREA)

Abstract

本发明公开了一种马铃薯淀粉加工废渣发酵装置与方法，装置包括带有无菌气体过滤排放口的罐体，罐体外部设置自动接菌箱，内部设置搅拌装置和气体自动交换装置；自动接菌箱安装于罐体侧上方，其内部设置菌液瓶和酒精灯，菌液瓶与连通罐体内部的接菌口通过密闭管道连接，向发酵体系送入菌液，酒精灯设置于接菌口下方对接菌口进行加热；气体自动交换装置包括压力传感器和若干空气分布器，罐体外部的无菌空气设备通过无菌空气进口与各空气分布器连通，向发酵体系输入无菌空气；压力传感器安装于无菌气体过滤排放口，向PLC控制系统提供实时气压信号，控制无菌气体过滤排放口的气体排放，最终解决现有发酵缺乏合适装置以及发酵效率不高的问题。

The invention discloses a device and method for fermenting potato starch processing waste residue. The device comprises a tank body with a sterile gas filtering and discharging port, an automatic bacteria inoculation box is arranged outside the tank body, and a stirring device and an automatic gas exchange device are arranged inside the tank body; the automatic bacteria inoculation box is installed on the upper side of the tank body, a bacteria liquid bottle and an alcohol lamp are arranged inside the automatic bacteria inoculation box, the bacteria liquid bottle is connected to the bacteria inoculation port connected to the inside of the tank body through a closed pipeline, and bacteria liquid is fed into a fermentation system, and the alcohol lamp is arranged below the bacteria inoculation port to heat the bacteria inoculation port; the automatic gas exchange device comprises a pressure sensor and a plurality of air distributors, the sterile air equipment outside the tank body is connected to each air distributor through a sterile air inlet, and sterile air is input into the fermentation system; the pressure sensor is installed at the sterile gas filtering and discharging port, provides a real-time air pressure signal to a PLC control system, controls the gas discharge of the sterile gas filtering and discharging port, and finally solves the problems of lack of suitable devices and low fermentation efficiency in the existing fermentation.

Description

Potato starch processing waste residue fermentation device and method
Technical Field
The invention belongs to the technical field of agricultural waste treatment, and particularly relates to a potato starch processing waste residue fermentation device and method.
Background
The potato starch processing waste residue is a main byproduct in the potato starch production process, and the yield of the waste residue is about 7 times of that of the refined starch. Along with starch production, the problem of environmental pollution caused by waste residues is increasingly remarkable, and the waste residues become a limiting factor for the development of starch processing enterprises. The waste residue contains a large amount of organic matters such as starch, cellulose, pectin, protein and the like, so that the waste residue has great utilization value, and the most common utilization mode at present is to ferment the waste residue into the bio-organic fertilizer.
However, the existing fermentation device can only ferment for a single raw material, and the fermentation efficiency is not high. And secondly, the changes of pH, temperature and pressure in the fermentation tank cannot be detected in real time in the fermentation process, and the changes cannot be adjusted in time. In addition, in the existing production mode, manual stirring is adopted, sealing is not tight, fermentation is easy to pollute, products are difficult to control, and the intensity is high during discharging, so that the method is not suitable for modern production requirements.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention aims to provide a potato starch processing waste residue fermentation device and method, so as to solve the problems of lack of proper device and low fermentation efficiency in the existing fermentation.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows:
a potato starch processing waste residue fermentation device comprises a tank body with a sterile gas filtering and discharging port, wherein an automatic bacteria receiving box is arranged outside the tank body, and a stirring device and a gas automatic exchange device are arranged inside the tank body;
The automatic bacteria receiving box is arranged above the side of the tank body, a bacteria liquid bottle and an alcohol lamp are arranged in the automatic bacteria receiving box, the bacteria liquid bottle is connected with a bacteria receiving port communicated with the inside of the tank body through a closed pipeline, bacteria liquid is fed into the fermentation system, and the alcohol lamp is arranged below the bacteria receiving port and is used for heating the bacteria receiving port;
The automatic gas exchange device comprises a pressure sensor and a plurality of air distributors, wherein sterile air equipment outside the tank body is communicated with each air distributor through a sterile air inlet and is used for inputting sterile air into a fermentation system, the pressure sensor is arranged at the sterile gas filtering and discharging port and is used for providing a real-time air pressure signal for a PLC control system, and the PLC control system is used for controlling the gas discharge of the sterile gas filtering and discharging port according to the real-time air pressure signal.
In one embodiment, the stirring device comprises a stirring shaft vertically arranged in the tank body, a propelling stirring paddle is vertically arranged on the stirring shaft, the propelling stirring paddle comprises a positive running stirring paddle and a negative running stirring paddle, and the positive running stirring paddles and the negative running stirring paddles are distributed on the stirring shaft in a staggered manner.
In one embodiment, the propeller is a three-blade propeller, and the blade surface and the front end of the blade are both wavy.
In one embodiment, the impeller is connected with the stirring shaft link ring by the impeller root, the inner wall of the stirring shaft link ring is provided with a tooth sleeve, and the impeller is screwed on the stirring shaft by the tooth sleeve and meshed with the tooth groove on the outer side of the stirring shaft.
In one embodiment, the positive and negative operation of the blade positive and blade negative operation paddles on the stirring shaft is achieved by means of a gear backdrive, differential backdrive, dual shaft nested backdrive, or independent drive.
In one embodiment, the gear reverse transmission is realized through a planetary gear mechanism or an idler gear set, the planetary gear mechanism is used for fixedly mounting a sun gear on a stirring shaft, fixedly mounting a blade negative-running stirring paddle on an outer gear ring, directly and fixedly connecting the blade positive-running stirring paddle on the stirring shaft, the idler gear set is used for driving a driving gear through the stirring shaft, an idler gear is additionally arranged in the middle of the idler gear set, then the idler gear is meshed with a driven gear of the blade negative-running stirring paddle, and the blade positive-running stirring paddle is directly and fixedly connected on the stirring shaft.
In one embodiment, a temperature sensor and a pH meter are arranged in the tank body, the PLC control system adjusts the temperature to 28-30 ℃ through a jacket type heat exchanger arranged on the inner wall of the tank body according to real-time temperature, pH value and pressure, the pH value is adjusted to 6.5-7.5 through an acid-base tank, sterile air is input into the tank through a sterile air inlet and an air distributor when the pressure in the tank is lower than the lower limit of the set pressure threshold range, and gas generated by fermentation is discharged through a sterile gas filtering discharge port when the pressure in the tank is higher than the upper limit of the set pressure threshold range.
In one embodiment, the air distributor outlet is arranged horizontally, obliquely downwards or vertically downwards, and the air outlet of the air distributor is provided with small fan blades.
The invention also provides a fermentation method based on the potato starch processing waste residue fermentation device, potato starch processing waste residue with the water content of more than 80% is put into a tank body, fermentation conditions are set to be 28-30 ℃ and 0.05-0.08Mpa, the fermentation tank is operated for 12 hours according to the fermentation conditions, and bacterial liquid is added after the fermentation conditions in materials are stable.
In one embodiment, the bacterial liquid is a liquid obtained by inoculating a bacterial strain monoclonal colony into an LB liquid culture medium, placing the liquid on a shaking table at a culture temperature of 30 ℃ for 3 days at 120 revolutions per minute, wherein the bacterial strain of effective live bacteria is pseudomonas aeruginosa which is classified and named Pseudomonas chlororaphis, the pseudomonas aeruginosa is preserved in China general microbiological culture Collection center with an address of North Star Xiu No. 1, no. 3 in the Korean yang area of Beijing, and the preservation date is 2011, 12 months and 20 days, and the preservation number is CGMCC No.5628.
In one embodiment, the inoculum size used is 0.8% of the material volume and the concentration of the inoculum is OD 600 = 1.0.
Compared with the prior art, the invention has the beneficial effects that:
1. The automatic bacteria receiving box is arranged on the tank body, and bacteria liquid is sent into the tank through the closed pipeline, so that aseptic operation is realized, and the pollution of mixed bacteria and bacteriophage is avoided.
2. The automatic gas exchange device is arranged, so that the sterile exchange of gas in the fermentation process can be realized, the stable air pressure required by the fermentation in the tank is maintained, the materials and the bacterial strain in the tank are fully fermented, and the labor input is reduced.
3. The invention improves the stirring effect by stirring in the forward direction and the reverse direction simultaneously.
4. The invention uses a PLC control system to automatically regulate and control the temperature, the pressure and the pH value, thereby realizing long-term continuous operation.
5. The invention adds specific potassium-dissolving, phosphorus-dissolving and nitrogen-fixing bacteria liquid in the fermentation process, thereby improving the fermentation effect.
Drawings
Fig. 1 is a schematic diagram of the structure of the present invention.
FIG. 2 is a schematic view of the structure of the stirring device of the present invention.
Fig. 3 is a schematic view of the installation structure of the propeller of the present invention.
Fig. 4 is a schematic view of the blade shape and structure of the propeller of the present invention.
Detailed Description
The invention will be described in further detail with reference to the drawings and examples. Other advantages and advantages of the present invention will become apparent to those skilled in the art from the following detailed description, which, by way of illustration, is to be read in connection with certain specific embodiments, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
As shown in fig. 1, the invention relates to a potato starch processing waste residue fermentation device, which comprises a tank body 1, wherein the tank body 1 is preferably of a cylindrical structure for convenient operation, and a stirring device and the like can be arranged in the tank body to stir materials in the fermentation process. The base structure of the tank is characterized in that a feed inlet 11 is positioned at the top of the tank body 1 and has a sight glass function, a discharge outlet 12 is positioned obliquely downwards at the bottom of the tank body 1, and a lighting device 13 is arranged at the top end in the tank. The feed inlet 11 has the function of a sight glass and is matched with the lighting equipment 13 for use, so that the fermentation condition can be observed conveniently.
The key point of the invention is that on one hand, considering the promotion effect of the strain in fermentation neglected in the prior art, an automatic bacteria inoculation box 9 is arranged outside the tank body 1. On the other hand, considering that the prior art ignores the adverse effect of fermentation gas on the long-term operation of a fermentation system, an automatic gas exchange device is arranged inside the tank body 1.
Wherein, the automatic bacteria receiving box 9 is a box body which can be sealed and is arranged at the upper position of the outer wall of the tank body 1, and a bacteria liquid bottle 9-4 and an alcohol lamp 9-2 are arranged in the automatic bacteria receiving box. The side wall of the tank body 1 is provided with a fungus receiving port 9-3, the fungus receiving port 9-3 is positioned in an automatic fungus receiving box 9, a fungus liquid bottle 9-4 is connected through a closed pipeline, and a small pressure pump 9-5 can be arranged, so that fungus liquid is directly fed into a fermentation system in the tank, and pollution is avoided. The alcohol lamp 9-2 is arranged below the inoculation port 9-3 to heat the inoculation port, thereby creating a sterile environment to ensure sterile operation and ensure that fermentation is pollution-free. Illustratively, the small-sized pressure pump 9-5 may be controlled by the pressure pump switch 9-1, and the alcohol burner 9-2 may be replaced by other heating devices such as an infrared inoculating loop sterilizer, etc.
The automatic gas exchange device comprises a pressure sensor 7 and a plurality of air distributors 6. A sterile air inlet 5 and a sterile gas filtration discharge 8 are provided on the tank 1, wherein the sterile air inlet 5 communicates with each air distributor 6, and a pressure sensor 7 is preferably mounted on the sterile gas filtration discharge 8 and communicates with a PLC control system 14. Therefore, sterile air equipment outside the tank body 1 can input sterile air into the fermentation system through the sterile air inlet 5 and each air distributor 6, so that no impurity bacteria pollution is ensured in the fermentation process. The PLC control system 14 can control the gas discharge of the sterile gas filter discharge port 8 according to the real-time gas pressure signal provided by the pressure sensor 7. In particular, the aseptic gas filtering and discharging port 8 is provided with an electric valve, an air pump and the like.
According to the structure, the invention can manually or automatically add the bacterial liquid into the fermentation system in the tank according to the needs, so as to realize co-fermentation of waste residues and bacterial strains, improve the fermentation effect and have convenient addition mode. And can automatically control the exhaust or the introduction of sterile gas according to the real-time air pressure signal, maintain a good fermentation environment and ensure the long-term stable operation of the system.
In one embodiment of the invention, the bottom of the inner cavity of the tank body 1 is arranged in a downward concave arc-shaped structure so as to well collect fermentation products. The fermentation product in the tank body 1 is discharged along the discharge hole 12, so that the rotation of the stirring shaft 2 can be ensured, the discharge of the discharge hole 12 is not influenced, and the accumulation of materials can be reduced by the arc-shaped tank bottom.
In one embodiment of the invention, referring to fig. 2, the stirring device comprises a stirring shaft 2 vertically arranged in the tank 1, wherein the stirring shaft 2 is in output connection with a motor 3 outside the tank and can rotate under the driving action of the motor 3. The stirring shaft 2 is vertically provided with the push type stirring paddles 4, the push type stirring paddles 4 rotate synchronously along with the stirring shaft 2 to stir a fermentation system, materials in the inner layer and the outer layer are uniformly stirred, the temperature of the inner layer and the outer layer of the materials is kept approximately the same, fermentation unevenness of a fermented product is avoided, a proper growth environment is provided for strains, and therefore fermentation efficiency is improved. Unlike the existing stirring mode, the propelling stirring paddle 4 of the present embodiment includes a positive-running stirring paddle 15 and a negative-running stirring paddle 16, which can rotate positively and negatively, respectively, and the positive-running stirring paddles 15 and the negative-running stirring paddles 16 are alternately distributed on the stirring shaft 2.
Through foretell positive and negative rotation stirring, can carry out more abundant stirring to fermented material, ensure that the material intensive mixing makes the temperature of each component of mixed material keep approximately the same, reduces the influence of temperature to fermentation, provides suitable environment for the fermentation of microorganism to increase the required dissolved oxygen volume of fermentation. The temperature of the inner layer and the outer layer of the mixed material is equivalent, the fermentation is uniform, and the fermentation efficiency is increased.
In one embodiment of the present invention, referring to fig. 3, a specific mounting structure of the propeller blades 4 is shown, wherein the blade root is connected to the shaft link ring 19, and each propeller blade 4 of the same layer is connected to the outer wall of the same shaft link ring 19. The inner wall of the stirring shaft link ring 19 is provided with a tooth sleeve 18, and the tooth sleeve 18 can be screwed on the stirring shaft 2 and synchronously rotate under the drive of the stirring shaft 2. Specifically, a tooth slot may be provided on the outer side of the stirring shaft 2, and the tooth slot is meshed with the tooth sleeve 18, so as to realize transmission.
In one embodiment of the invention, referring to fig. 4, the propelling type stirring paddle 4 is a three-blade propelling type stirring paddle, and the blade surface and the front end of the blade are both wavy, and the shape structure can enlarge the contact area with the fermentation liquor, so that the flowability of the mixed material is enhanced, the innermost material and the outermost material are convenient to mix, the inner and outer materials can exchange heat, the materials are fully fermented finally, the stirring effect and the fermentation efficiency are improved, and the problems of insufficient stirring of the materials and unstable products in the fermentation process are solved.
In one embodiment of the present invention, to connect the blade positive-running stirring paddles 15 and the blade negative-running stirring paddles 16 on the same stirring shaft 2, the connection can be realized through a gear reverse transmission mode, a differential reverse transmission mode, a double-shaft nested reverse driving mode or an independent driving mode. The planetary gear mechanism has compact structure, high torque transmission efficiency, simple structure and low cost. The invention is preferably implemented in two ways.
When the planetary gear mechanism is adopted, the sun gear is fixedly arranged on the stirring shaft 2, the blade negative-direction running stirring paddle 16 is fixedly arranged on the outer gear ring, reverse rotation is realized through reverse engagement of the planet gears, and the blade positive-direction running stirring paddle 15 is directly and fixedly connected on the stirring shaft 2 and rotates along with the shaft in the positive direction.
When the idler gear is adopted, the stirring shaft drives a driving gear, the idler gear is additionally arranged in the middle of the driving gear, and then the driven gear of the stirring paddle 16 which runs in the negative direction of the blade is meshed, so that the reverse rotation is realized, and the stirring paddle 15 which runs in the positive direction of the blade is directly and fixedly connected to the stirring shaft 2 and rotates in the positive direction along with the shaft.
In one embodiment of the application, a jacketed heat exchanger 10 is arranged on the inner wall of the tank body 1, and can realize the regulation and control of the temperature in the tank, namely the increase or decrease, by introducing different heat exchange media, so that the fermentation system keeps constant temperature. Meanwhile, a temperature sensor and a pH meter are arranged in the tank, so that the PLC control system 14 can adjust the temperature to a set range through the jacket type heat exchanger 10 arranged on the inner wall of the tank body 1 according to the real-time temperature, pH and pressure, adjust the pH to the set range through an externally connected acid-base tank, input sterile air into the tank through the sterile air inlet 5 and the air distributor 6 when the pressure in the tank is lower than the lower limit of the set pressure threshold range, and discharge gas generated by fermentation through the sterile gas filtering discharge port 8 when the pressure in the tank is higher than the upper limit of the set pressure threshold range, so as to adjust the pressure in the tank to the set range.
In one embodiment of the invention, the air distributor 6 outlet is arranged horizontally, or obliquely downwards, or vertically downwards, to prevent viscous fermented material from blocking the outlet. Further, small-sized fan blades can be arranged at the air outlet of the air-cooled fermentation tank, and can rotate along with the air flow when ventilation is performed, so that surrounding fermentation materials are discharged, and sterile air is fully mixed with the fermentation materials. The outlet may also be provided with an anti-blocking flap which is designed to be opened only in the direction of the air flow, to be opened under the pressure of the air flow, and to be automatically closed in the absence of air flow, preventing the material from blocking the outlet. In the fermentation process, the pressure sensor 7 detects the air pressure in the tank body 1 in real time, ensures that the sterile gas filtering and discharging port 8 timely discharges fermentation generated gas, maintains the stable air pressure required by the fermentation in the tank body 1, and reduces the influence on the fermentation of internal strains.
The fermentation method of the fermentation device can utilize devices such as a mechanical pump and the like to input potato starch processing waste residue with the water content of more than 80% into the tank body 1 through the feed inlet 11, the fermentation condition is set to be that the optimal temperature is 28-30 ℃, the optimal pH is 6.5-7.5, the optimal pressure is 0.05-0.08Mpa, the fermentation tank is operated for 12 hours according to the fermentation condition, and after the fermentation condition in materials is stable, the small pressure pump 9-5 and the alcohol lamp 9-2 are started, and fermentation bacteria liquid is added.
During fermentation, the above-described strict control of fermentation conditions, in particular, can be performed by the PLC control system 14:
when the fermentation temperature is higher than 30 ℃, the jacketed heat exchanger 10 is controlled to be automatically started, and the fermentation temperature of the materials is always controlled to be in an optimal temperature range by injecting cold water and the like to cool the fermentation materials.
Normal fermentation is performed within the pH range of 6.5-7.5, when the pH is beyond the pH range, for example, when the pH is lower than the pH range, naOH is injected into the tank body 1 by the PLC control system 14 through an acid-base tank controlled by the PLC control system, so that the pH is raised, and when the pH is higher than the pH range, the pH is lowered by the PLC control system 14 through the acid-base tank controlled by the PLC control system 14, so that the fermentation pH of materials is always controlled within the optimal range.
Normal fermentation is carried out within the range of 0.05-0.08Mpa, when the fermentation pressure exceeds the range, for example, the fermentation pressure is lower than 0.08Mpa, gas generated by fermentation is controlled to be discharged through a sterile gas filtering discharge port 8, and when the fermentation pressure exceeds the range, for example, the fermentation temperature of materials is always controlled within an optimal temperature threshold value, wherein sterile air is input into a fermentation system through a sterile air inlet 5 and each air distributor (6).
According to the structure and the control strategy, the invention can automatically monitor and record the temperature and pH change in the tank body 1, and the PLC control system automatically adjusts the temperature to keep the constant temperature in the fermentation, so that the operation is concise, and excessive manual intervention is not needed;
In the embodiment of the invention, the tank 1 is fully loaded, i.e. the potato starch processing waste residue added to the tank fills the tank, for example 500 liters of material can be added when the volume of the tank is 500 liters. Illustratively, the tank 1 may be made of stainless steel, and has a smooth inner surface and is not easy to wall.
In one embodiment of the invention, a special bacterial solution is adopted, wherein a bacterial strain monoclonal colony is inoculated in LB liquid culture medium and placed on a shaking table with the culture temperature of 30 ℃ for 120 revolutions per minute, the liquid is cultured for 3 days, and the bacterial strain of effective living bacteria is pseudomonas aeruginosa (Pseudomonas chlororaphis), the collection center CGMCC and the number 5628. The prior art shows that the strain is potassium-decomposing bacteria, the potassium-decomposing amount in the culture solution is about 3.1 mug/mL, and the strain is clearly defined as potassium-decomposing bacteria in patent CN105254356A and is used for biological organic fertilizer.
The applicant finds that the strain has the function of decomposing inorganic phosphorus through further test measurement, and the quantitative measurement of the strain capacity is carried out as follows:
And (3) quantitative determination of dissolved phosphorus, namely filling 50mL of sterilized inorganic phosphorus liquid culture medium into a 100mL triangular flask, respectively inoculating 2.5mL of bacterial suspension to be tested into the inorganic phosphorus liquid culture medium, setting a control group, repeating for 3 times, culturing for 5 days at 30 ℃ and 120r/min, centrifuging the culture solution for 10min at 4 ℃ and 10000r/min, and taking supernatant and measuring the content of effective phosphorus by using a molybdenum-antimony colorimetric method.
The nitrogen fixation capacity of the strain is expressed as the activity of the azotase, and the activity of the azotase of the strain is measured by adopting an acetylene (C 2H2) reduction method. Strains were inoculated into 5mL of semi-solid NFM medium (serum bottle, 15mL in specification) with an inoculating loop, each strain was repeated 3 times, the culture medium without inoculation was used as a control, the culture was closed with a cotton plug, after 48 hours of culture at 28 ℃, the cotton plug was replaced with a rubber plug, 1mL of gas was extracted with a sterile syringe, then 1mL of C 2H2 gas was injected, and the culture was carried out in a 28 ℃ incubator for 48 hours. 50 μl of the mixed gas was extracted from the serum bottle using a 100 μl microsyringe and injected into a gas sample injection column of a gas chromatograph, and the peak time and peak area of C 2H2 were recorded and observed, and the C 2H2 content was calculated, and the results are shown in table 1.
And (3) quantitative determination of potassium solution, namely inoculating the single flora obtained by separation and purification into an improved liquid culture medium, and carrying out shake culture for 10d under the conditions of 30 ℃ and 120r/min by taking potassium feldspar powder as a unique potassium source. 10.0mL of the culture solution was centrifuged at 8 000r/min for 8min, the supernatant was collected, and the amount of potassium released in the supernatant was measured by atomic absorption spectrophotometry, and the measurement was repeated 3 times as compared with a blank group without inoculating potassium releasing bacteria, and the results of each measurement were recorded, and the results are shown in Table 1.
TABLE 1
The result shows that the phosphorus dissolving capacity of the strain in the culture solution is 365.9 mu g/mL, the nitrogen fixing capacity is 483.4 (nmol C 2H4h-1·mL-1), and the residual organic matters in the potato starch processing waste residue can be utilized to generate various secondary metabolites, so that the fermentation product of the strain has rich life-promoting substances. In addition, the strain is plant growth promoting bacteria, can fully adapt to the fermentation environment, improves the content of beneficial microorganism viable bacteria in the fermentation product, and achieves the effect of effectively promoting plant growth.
In one embodiment of the invention, when co-fermenting with potato starch processing waste residues, the inoculation amount used by the fermentation system is 0.8% of the volume of the material, and the concentration of bacterial liquid is OD 600 =1.0. The final co-fermentation product of potato starch processing waste residue and microorganisms is a high-efficiency bio-organic fertilizer.
The above embodiments are only exemplary embodiments of the present application and are not intended to limit the present application, the scope of which is defined by the claims. Various modifications and equivalent arrangements of this application will occur to those skilled in the art, and are intended to be within the spirit and scope of the application.

Claims (10)

1.一种马铃薯淀粉加工废渣发酵装置，其特征在于，包括带有无菌气体过滤排放口(8)的罐体(1)，所述罐体(1)外部设置自动接菌箱(9)，内部设置搅拌装置和气体自动交换装置；1. A potato starch processing waste residue fermentation device, characterized in that it comprises a tank body (1) with a sterile gas filter discharge port (8), an automatic inoculation box (9) is arranged outside the tank body (1), and a stirring device and a gas automatic exchange device are arranged inside the tank body (1); 所述自动接菌箱(9)安装于罐体(1)侧上方，其内部设置菌液瓶(9-4)和酒精灯(9-2)，所述菌液瓶(9-4)与连通罐体(1)内部的接菌口(9-3)通过密闭管道连接，向发酵体系送入菌液，所述酒精灯(9-2)设置于接菌口(9-3)下方对接菌口(9-3)进行加热；The automatic inoculation box (9) is installed on the upper side of the tank body (1), and a bacterial liquid bottle (9-4) and an alcohol lamp (9-2) are arranged inside the automatic inoculation box (9). The bacterial liquid bottle (9-4) is connected to the inoculation port (9-3) inside the tank body (1) through a closed pipe to feed bacterial liquid into the fermentation system. The alcohol lamp (9-2) is arranged below the inoculation port (9-3) to heat the inoculation port (9-3); 所述气体自动交换装置包括压力传感器(7)和若干空气分布器(6)，罐体(1)外部的无菌空气设备通过无菌空气进口(5)与各所述空气分布器(6)连通，向发酵体系输入无菌空气；所述压力传感器(7)安装于所述无菌气体过滤排放口(8)，向PLC控制系统(14)提供实时气压信号，所述PLC控制系统(14)根据所述实时气压信号，控制无菌气体过滤排放口(8)的气体排放。The automatic gas exchange device comprises a pressure sensor (7) and a plurality of air distributors (6). The sterile air equipment outside the tank body (1) is connected to each of the air distributors (6) through a sterile air inlet (5) to input sterile air into the fermentation system. The pressure sensor (7) is installed at the sterile gas filter outlet (8) to provide a real-time air pressure signal to the PLC control system (14). The PLC control system (14) controls the gas discharge from the sterile gas filter outlet (8) according to the real-time air pressure signal. 2.根据权利要求1所述马铃薯淀粉加工废渣发酵装置，其特征在于，所述搅拌装置包括竖直安装在所述罐体(1)内的搅拌轴(2)，所述搅拌轴(2)上垂直安装有推进式搅拌桨(4)，所述推进式搅拌桨(4)包括正向运转搅拌桨(15)和负向运转搅拌桨(16)，所述叶片正向运转搅拌桨(15)和叶片负向运转搅拌桨(16)在所述搅拌轴(2)上交错分布。2. The potato starch processing waste fermentation device according to claim 1 is characterized in that the stirring device includes a stirring shaft (2) vertically installed in the tank body (1), and a propulsion stirring paddle (4) is vertically installed on the stirring shaft (2), and the propulsion stirring paddle (4) includes a positive running stirring paddle (15) and a negative running stirring paddle (16), and the blades of the positive running stirring paddle (15) and the blades of the negative running stirring paddle (16) are staggered on the stirring shaft (2). 3.根据权利要求2所述马铃薯淀粉加工废渣发酵装置，其特征在于，所述推进式搅拌桨(4)为三叶推进式搅拌桨，其叶片表面和叶片前端均为波浪形。3. The potato starch processing waste residue fermentation device according to claim 2 is characterized in that the propulsion stirring paddle (4) is a three-blade propulsion stirring paddle, and its blade surface and blade front end are both wavy. 4.根据权利要求2或3所述马铃薯淀粉加工废渣发酵装置，其特征在于，所述叶片正向运转搅拌桨(15)和叶片负向运转搅拌桨(16)在所述搅拌轴(2)上的正向运转和负向运转，通过齿轮反向传动、差速器反向传动、双轴嵌套反向驱动或独立驱动的方式实现。4. The potato starch processing waste fermentation device according to claim 2 or 3 is characterized in that the positive operation and negative operation of the blade-positively rotating stirring paddle (15) and the blade-negatively rotating stirring paddle (16) on the stirring shaft (2) are achieved by gear reverse transmission, differential reverse transmission, double-shaft nested reverse drive or independent drive. 5.根据权利要求4所述马铃薯淀粉加工废渣发酵装置，其特征在于，所述齿轮反向传动，通过行星齿轮机构或惰轮齿轮组实现；所述行星齿轮机构，将太阳轮固定安装于搅拌轴(2)，将叶片负向运转搅拌桨(16)固定安装于外齿圈，将叶片正向运转搅拌桨(15)直接固定连接在搅拌轴(2)上；所述惰轮齿轮组，以搅拌轴驱动一个主动齿轮，中间加装惰轮，再啮合叶片负向运转搅拌桨(16)的从动齿轮，所述叶片正向运转搅拌桨(15)直接固定连接在搅拌轴(2)上。5. The potato starch processing waste fermentation device according to claim 4 is characterized in that the gear reverse transmission is realized by a planetary gear mechanism or an idler gear set; the planetary gear mechanism fixes the sun gear on the stirring shaft (2), fixes the blade negative rotation stirring paddle (16) on the outer gear ring, and directly fixes the blade positive rotation stirring paddle (15) on the stirring shaft (2); the idler gear set drives a driving gear with the stirring shaft, an idler gear is added in the middle, and then meshes with the driven gear of the blade negative rotation stirring paddle (16), and the blade positive rotation stirring paddle (15) is directly fixedly connected to the stirring shaft (2). 6.根据权利要求1所述马铃薯淀粉加工废渣发酵装置，其特征在于，所述罐体(1)内设置温度传感器和pH计，所述PLC控制系统(14)根据实时的温度、pH和压力，通过罐体(1)内壁设置的夹套式热交换器(10)调节温度至28-30℃，通过酸碱罐调节pH至6.5-7.5，在罐内压力低于设定的压力阈值范围下限时，通过无菌空气进口(5)和空气分布器(6)向罐内输入无菌空气，在罐内压力高于设定的压力阈值范围上限时，通过无菌气体过滤排放口(8)排放发酵产生的气体。6. The potato starch processing waste fermentation device according to claim 1 is characterized in that a temperature sensor and a pH meter are arranged in the tank body (1); the PLC control system (14) adjusts the temperature to 28-30°C through a jacketed heat exchanger (10) arranged on the inner wall of the tank body (1) according to the real-time temperature, pH and pressure, and adjusts the pH to 6.5-7.5 through an acid-base tank; when the pressure in the tank is lower than the lower limit of the set pressure threshold range, sterile air is input into the tank through a sterile air inlet (5) and an air distributor (6); when the pressure in the tank is higher than the upper limit of the set pressure threshold range, the gas generated by the fermentation is discharged through a sterile gas filter discharge port (8). 7.根据权利要求1所述马铃薯淀粉加工废渣发酵装置，其特征在于，所述空气分布器(6)出口水平设置，或倾斜向下设置，或垂直向下设置；所述空气分布器(6)的空气出口处，装有小型扇叶。7. The potato starch processing waste fermentation device according to claim 1 is characterized in that the outlet of the air distributor (6) is arranged horizontally, or inclined downward, or vertically downward; and a small fan blade is installed at the air outlet of the air distributor (6). 8.基于权利要求1所述马铃薯淀粉加工废渣发酵装置的发酵方法，其特征在于，向罐体(1)投入含水率80％以上的马铃薯淀粉加工废渣，设定发酵条件为：温度28-30℃，pH 6.5-7.5，压力0.05-0.08Mpa，按照所述发酵条件运行发酵罐12小时，待物料中发酵条件稳定后，加入菌液，所述菌液中有效活菌的菌株为绿针假单胞菌(Pseudomonas chlororaphis)，保藏中心CGMCC，编号5628。8. A fermentation method based on the potato starch processing waste fermentation device according to claim 1, characterized in that potato starch processing waste with a moisture content of more than 80% is added to the tank body (1), and the fermentation conditions are set as follows: temperature 28-30°C, pH 6.5-7.5, pressure 0.05-0.08Mpa, and the fermentation tank is operated for 12 hours according to the fermentation conditions. After the fermentation conditions in the material are stable, a bacterial solution is added, and the strain of effective live bacteria in the bacterial solution is Pseudomonas chlororaphis, which is collected by the CGMCC Collection Center and is numbered 5628. 9.根据权利要求8所述发酵方法，其特征在于，所述菌液为菌株单克隆菌落接种在LB液体培养基中，置于每分钟120转，培养温度为30摄氏度的摇床上培养3天时的液体。9. The fermentation method according to claim 8, characterized in that the bacterial liquid is a liquid obtained by inoculating a monoclonal colony of the strain in LB liquid culture medium and culturing it on a shaker at 120 revolutions per minute and 30 degrees Celsius for 3 days. 10.根据权利要求8所述发酵方法，其特征在于，所使用的接菌量为物料体积的0.8％，菌液的浓度为OD600＝1.0。10. The fermentation method according to claim 8, characterized in that the inoculation amount used is 0.8% of the volume of the material, and the concentration of the bacterial solution is OD600 = 1.0.
CN202510508999.9A 2025-04-22 2025-04-22 Potato starch processing waste residue fermentation device and method Pending CN120366031A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN121294127A (en) * 2025-10-30 2026-01-09 广东沁林机械设备实业有限公司 A fermentation device based on industrial automation

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN121294127A (en) * 2025-10-30 2026-01-09 广东沁林机械设备实业有限公司 A fermentation device based on industrial automation

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