CN120285630A - A device for collecting proteins secreted by stem cell differentiation based on precipitation method - Google Patents
A device for collecting proteins secreted by stem cell differentiation based on precipitation method Download PDFInfo
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- CN120285630A CN120285630A CN202510460543.XA CN202510460543A CN120285630A CN 120285630 A CN120285630 A CN 120285630A CN 202510460543 A CN202510460543 A CN 202510460543A CN 120285630 A CN120285630 A CN 120285630A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D21/00—Separation of suspended solid particles from liquids by sedimentation
- B01D21/18—Construction of the scrapers or the driving mechanisms for settling tanks
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D21/00—Separation of suspended solid particles from liquids by sedimentation
- B01D21/24—Feed or discharge mechanisms for settling tanks
- B01D21/245—Discharge mechanisms for the sediments
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D21/00—Separation of suspended solid particles from liquids by sedimentation
- B01D21/28—Mechanical auxiliary equipment for acceleration of sedimentation, e.g. by vibrators or the like
- B01D21/283—Settling tanks provided with vibrators
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
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- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
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- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention relates to the technical field of protein collecting devices, and discloses a protein collecting device for generating secretion by stem cell differentiation based on a precipitation method, which comprises a scraping plate, wherein the surface of the scraping plate is provided with a guide groove, and the bottom of the scraping plate is fixedly connected with a guide groove plate; according to the invention, the scraping mechanism is arranged, when the scraping plate rotates, the inner wall of the collecting cylinder is scraped, when the scraping plate rotates, residual protein on the inner wall of the collecting cylinder is scraped to the inner wall of the guide groove through rotating inertia, when the protein flows to the guide groove, the protein flows downwards along the guide groove, and the protein on the inner wall of the guide groove flows downwards rapidly in cooperation with vibration sense generated by impact of the contact telescopic rod on the surface of the collecting cylinder, so that the protein on the inner wall of the collecting cylinder is collected rapidly, damage is reduced by collecting the residual protein effectively, cleaning time is saved, and efficiency is improved for next use.
Description
Technical Field
The invention relates to the technical field of protein collection equipment, in particular to a protein collection device for stem cell differentiation and secretion based on a precipitation method.
Background
Proteins are one of the most fundamental molecular components in organisms, playing an important role in regulating cellular functions and signaling, and proteomics plays an important role in biopharmaceutical development, and can be used to identify and quantify drug candidates, monitor protein expression and quality consistency, and the like. The application of proteomics can improve the efficiency and success rate of drug development.
In the prior art, when the protein is precipitated, the protein needs to be collected, but after the protein is taken away from the collecting device, some protein still remains in the collecting device and is adhered to the inner wall of the collecting device, and when the residual protein is damaged after a long time, the residual protein needs to be collected, and the protein is easily damaged by manual collection.
Disclosure of Invention
The present invention is directed to a device for collecting secreted proteins generated by stem cell differentiation based on precipitation method, which solves the above-mentioned problems of the prior art.
In order to solve the technical problems, the invention is realized by the following technical scheme:
The invention relates to a protein collecting device for stem cell differentiation and secretion based on a precipitation method, which comprises a collecting cylinder, wherein the surface of the collecting cylinder is fixedly connected with supporting legs, the bottoms of the supporting legs are fixedly connected with bottom plates, the surfaces of the bottom plates are fixedly connected with supporting frames, and the tops of the supporting frames are fixedly connected with motors;
The vibration mechanism comprises a moving plate, the surface of the moving plate is rotationally connected with a driven push rod, and one end of the driven push rod, which is far away from the moving plate, is rotationally connected with a contact telescopic rod;
The scraping mechanism comprises a scraping plate, a guide groove is formed in the surface of the scraping plate, and a guide groove plate is fixedly connected to the bottom of the scraping plate;
The collecting mechanism comprises a threaded plate, both ends of the threaded plate are fixedly connected with scraping rings, and the bottom of the threaded plate is rotationally connected with a pushing plate.
Further, the number of the supporting frames is four, the four supporting frames are symmetrically arranged by taking the bottom plate as a center, the bottom of the inner wall of the collecting barrel is provided with a containing hole, and the number of the supporting feet is three.
Further, the vibration mechanism comprises a transmission shaft, a threaded ring is fixedly connected to the surface of the transmission shaft, a swivel is fixedly connected to the surface of the threaded ring, an extrusion bent rod is fixedly connected to the bottom of the swivel, a chute plate is fixedly connected to the inner wall of the support frame, a stressed inclined plate is fixedly connected to the top of the movable plate, and a reset spring is fixedly connected to the bottom of the movable plate.
Further, the end of the transmission shaft is fixedly connected with the output end of the motor, the two ends of the moving plate are in sliding connection with the inner wall of the chute plate, one end of the extrusion bent rod, which is far away from the swivel, is in contact with the surface of the stressed sloping plate, and one end of the contact telescopic rod, which is far away from the driven push rod, is fixedly connected with the inner wall of the support frame.
Further, scrape material mechanism and include the pivot, the fixed surface of pivot is connected with driven ring, from the fixed surface of moving ring is connected with the bull stick, and the bull stick is kept away from the one end fixedly connected with clearance board of moving ring, and clearance board's fixed surface is connected with the cleaning brush.
Further, the tip fixed connection of pivot and the tip fixed connection of transmission shaft, the surface of scraper blade and the fixed connection of surface of bull stick, the one side that the scraper blade was kept away from the bull stick and the inner wall contact of a collection section of thick bamboo, the one end that the cleaning brush was kept away from the cleaning plate and the inner wall contact of a collection section of thick bamboo.
Further, the collection mechanism comprises a threaded rod, one end of the pushing plate, which is far away from the threaded plate, is rotationally connected with a collection contraction ring, the bottom of the threaded plate is fixedly connected with a limit spring, the bottom of the collection barrel is provided with a storage box, and the inner wall of the collection barrel is fixedly connected with a drainage sloping plate.
Further, the one end that the transmission shaft was kept away from to the pivot is with the tip fixed connection of threaded rod, collects the bottom contact of shrink ring and the bottom of collection section of thick bamboo inner wall, scrapes the outer wall of ring and the inner wall contact of collection section of thick bamboo, and the one end that the pivot was kept away from to the threaded rod rotates with the inner wall of collection section of thick bamboo to be connected, and the top of receiver contacts with the bottom of collection section of thick bamboo, the bottom of receiver and the surface contact of bottom plate.
The invention has the following beneficial effects:
According to the invention, by arranging the vibration mechanism, the motor is started to drive the transmission shaft to rotate at first, the threaded ring is driven to rotate when the transmission shaft rotates, the rotating ring is driven to rotate when the threaded ring rotates, the stressed inclined plate is extruded when the rotating ring rotates, the movable plate is pushed to slide downwards on the inner wall of the chute plate when the stressed inclined plate is extruded, the driven push rods are pushed to move in the directions away from each other when the movable plate slides, the driven push rods are pushed to extend towards the directions close to each other and are contacted with the surface of the collecting cylinder, the reset spring is extruded when the movable plate slides, the reset spring is contracted downwards when the reset spring is extruded, the driven push rods are pulled to return to the original position by the elastic force of the reset spring, the driven push rods drive the threaded ring to rotate simultaneously, the extruded bent rods are kept to rotate by the rotating ring, and are extruded by the stressed bent rods, the driven push rods are arranged in a plurality of directions, and a certain distance is spaced between the extruded bent rods, so that the stressed inclined plates are pushed to extend towards each other in the directions, the directions close to each other, the inclined plate is extruded by the reciprocating bent rods, the inclined plate is pushed by the elastic force of the reset spring, the reset spring is contracted downwards, the elastic force of the reset spring returns to the original position, and the inclined plate returns to the original position, and the driven plate returns to the original position, and moves the driven plate, and moves to the inner wall.
According to the invention, the scraping mechanism is arranged, the rotating shaft is driven to rotate when the transmission shaft rotates, the driven ring is driven to rotate when the rotating shaft rotates, the rotating rod is driven to rotate when the driven ring rotates, the cleaning plate is driven to rotate when the rotating rod rotates, and the cleaning brush is driven to rotate when the cleaning plate rotates, so that the inner wall of the collecting cylinder is cleaned, the labor force is saved, the scraping plate is driven to rotate when the bottom plate rotates, the inner wall of the collecting cylinder is scraped when the scraping plate rotates, residual protein on the inner wall of the collecting cylinder is scraped to the inner wall of the guide groove through rotating inertia when the scraping plate rotates, the protein flows downwards along the guide groove when flowing to the guide groove, and the protein on the inner wall of the guide groove is rapidly downwards caused by the shock generated by the impact of the contact telescopic rod, so that the protein on the inner wall of the collecting cylinder is rapidly collected, the damage is effectively reduced, the inner wall of the collecting cylinder is cleaned through the cleaning brush, the time is saved, and the efficiency is improved for the next use.
According to the invention, the collecting mechanism is arranged, the threaded rod is driven to rotate when the rotating shaft rotates, the threaded plate moves downwards when the threaded rod rotates, the scraping ring is pulled to move downwards when the threaded plate moves, the lower surface of the inner wall of the collecting cylinder is scraped downwards when the scraping ring moves, protein on the inner wall is scraped downwards, the protein flows downwards through the guide of the drainage inclined plate, at the moment, the threaded plate moves to a position where the surface of the threaded rod is not threaded, the threaded plate is kept still through the limit spring to limit, at the moment, the threaded rod keeps rotating, meanwhile, the scraping ring moves to the upper part of the drainage inclined plate and keeps still and is not contacted with the drainage inclined plate, the pushing plate is pushed to move downwards when the scraping ring moves downwards, and the collecting shrinkage ring is pushed to shrink towards the direction close to each other when the pushing plate moves, so that the protein flowing downwards is concentrated and pushed to the inner wall of the collecting box, and the residual protein is effectively collected, and the damage of the protein is reduced.
Of course, it is not necessary for any one product to practice the invention to achieve all of the advantages set forth above at the same time.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed for the description of the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of the overall structure of the present invention;
FIG. 2 is a schematic overall sectional view of the present invention;
FIG. 3 is a schematic view of the overall structure of the vibration mechanism of the present invention;
FIG. 4 is a schematic view of the driven pushrod according to the present invention;
FIG. 5 is a schematic view of the overall structure of the scraping mechanism of the invention;
FIG. 6 shows a flow guiding groove according to the present invention a schematic diagram of a plate structure;
FIG. 7 is a schematic view of the overall structure of the collecting mechanism of the present invention;
FIG. 8 is a schematic view of a scraper ring according to the present invention;
fig. 9 is a schematic structural view of the storage box of the present invention.
In the drawings, the list of components represented by the various numbers is as follows:
In the figure, 1, a collecting cylinder; 2, supporting legs, 3, a bottom plate, 4, a supporting frame, 5, a motor, 10, a vibrating mechanism, 11, a transmission shaft, 12, a threaded ring, 13, a swivel, 14, a squeezing bent rod, 15, a sliding groove plate, 16, a moving plate, 17, a stressed inclined plate, 18, a driven push rod, 19, a contact telescopic rod, 20, a return spring, 30, a scraping mechanism, 31, a rotating shaft, 32, a driven ring, 33, a rotating rod, 34, a cleaning plate, 35, a cleaning brush, 36, a scraping plate, 37, a diversion groove plate, 50, a collecting mechanism, 51, a threaded rod, 52, a threaded plate, 53, a scraping ring, 54, a pushing plate, 55, a collecting shrinkage ring, 56, a limiting spring, 57, a storage box, 58 and a drainage inclined plate.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to fig. 1-9, the invention discloses a protein collecting device for generating secretion by stem cell differentiation based on a precipitation method, which comprises a collecting cylinder 1, wherein the surface of the collecting cylinder 1 is fixedly connected with a supporting leg 2, the bottom of the supporting leg 2 is fixedly connected with a bottom plate 3, the surface of the bottom plate 3 is fixedly connected with a supporting frame 4, the top of the supporting frame 4 is fixedly connected with a motor 5, and the device further comprises;
The vibrating mechanism 10 comprises a moving plate 16, when the stressed inclined plate 17 is extruded, the moving plate 16 is pushed to slide downwards on the inner wall of the chute plate 15, the surface of the moving plate 16 is rotationally connected with a driven push rod 18, when the moving plate 16 slides, the driven push rod 18 is pushed to move in a direction away from each other, one end of the driven push rod 18 away from the moving plate 16 is rotationally connected with a contact telescopic rod 19, and when the driven push rod 18 moves, the contact telescopic rod 19 is pushed to extend in a direction approaching each other and is contacted with the surface of the collecting cylinder 1;
The scraping mechanism 30 comprises a scraping plate 36, the scraping plate 36 is driven to rotate while the bottom plate 3 rotates, a guide groove is formed in the surface of the scraping plate 36, the inner wall of the collecting cylinder 1 is scraped when the scraping plate 36 rotates, residual protein on the inner wall of the collecting cylinder 1 is scraped to the inner wall of the guide groove through rotating inertia while the scraping plate 36 rotates, the protein flows downwards along the guide groove when flowing to the guide groove, and the protein on the inner wall of the guide groove flows downwards in a rapid manner by matching with vibration sense generated by impact of the contact telescopic rod 19 on the surface of the collecting cylinder 1, and a guide groove plate 37 is fixedly connected to the bottom of the scraping plate 36;
The collecting mechanism 50, the collecting mechanism 50 includes the screw thread board 52, can make screw thread board 52 downwardly moving when threaded rod 51 rotates, the both ends fixedly connected with of screw thread board 52 scrape the ring 53, can pull when screw thread board 52 removes and scrape the ring 53 downwardly moving, can scrape the lower surface of the inner wall of the cylinder 1 down when scraping the ring 53 and scrape the protein of inner wall downwardly when scraping the ring 53 removal, the bottom rotation of screw thread board 52 is connected with the push plate 54, can promote the push plate 54 downwardly moving when scraping the ring 53 downwardly moving.
The quantity of support frame 4 is provided with four, and four support frames 4 use bottom plate 3 as central symmetry setting, and the storage hole has been seted up to the bottom of the inner wall of collection cylinder 1, and the quantity of supporting legs 2 is provided with three.
The vibration mechanism 10 comprises a transmission shaft 11, a motor 5 is started to drive the transmission shaft 11 to rotate, a threaded ring 12 is fixedly connected to the surface of the transmission shaft 11, the threaded ring 12 is driven to rotate when the transmission shaft 11 rotates, a rotating ring 13 is fixedly connected to the surface of the threaded ring 12, the rotating ring 13 is driven to rotate when the threaded ring 12 rotates, a compression bending rod 14 is fixedly connected to the bottom of the rotating ring 13, the rotating ring 13 drives the compression bending rod 14 to keep rotating, the stressed sloping plate 17 is compressed, the inner wall of a supporting frame 4 is fixedly connected with a chute plate 15, the top of a moving plate 16 is fixedly connected with the stressed sloping plate 17, the stressed sloping plate 17 is compressed when the rotating ring 13 rotates, a reset spring 20 is fixedly connected to the bottom of the moving plate 16, the reset spring 20 is compressed when the moving plate 16 slides, the reset spring 20 is compressed downwards, a driven push rod 18 is pushed to reciprocate when the moving plate 16 slides reciprocally, and the driven push a contact telescopic rod 19 to reciprocate, and slightly collide the surface of the collecting cylinder 1, so that the inner wall of the collecting cylinder 1 is vibrated, the stressed sloping plate 17 is compressed, the protein is adhered to the inner wall of the collecting cylinder 1, the residual protein is sucked downwards, and the residual protein is sucked downwards when the inner wall of the collecting cylinder is sucked.
The end of the transmission shaft 11 is fixedly connected with the output end of the motor 5, the transmission shaft 11 can drive the threaded ring 12 to rotate, the two ends of the moving plate 16 are slidably connected with the inner wall of the chute plate 15, at the moment, the moving plate 16 has no extrusion force, the moving plate can be reset to the original position through the elastic force of the reset spring 20, one end of the extrusion bent rod 14 far away from the swivel 13 is contacted with the surface of the stressed inclined plate 17, and the extrusion bent rods 14 are arranged in a plurality, and a certain distance is reserved between every two extrusion bent rods 14, so that the stressed inclined plate 17 can be extruded, the stressed inclined plate 17 pushes the moving plate 16 to slide reciprocally, when the extrusion bent rod 14 finishes extruding the stressed inclined plate 17, one end of the contact telescopic rod 19 far away from the driven push rod 18 is fixedly connected with the inner wall of the support frame 4, and meanwhile, the driven push rod 18 can pull the contact telescopic rod 19 to return to the original position.
The scraping mechanism 30 comprises a rotating shaft 31, the rotating shaft 31 is driven to rotate when the transmission shaft 11 rotates, a driven ring 32 is fixedly connected to the surface of the rotating shaft 31, the driven ring 32 is driven to rotate when the rotating shaft 31 rotates, a rotating rod 33 is fixedly connected to the surface of the driven ring 32, the rotating rod 33 is far away from a cleaning plate 34 which is fixedly connected with one end of the driven ring 32, a cleaning brush 35 is fixedly connected to the surface of the cleaning plate 34, and the cleaning brush 35 is driven to rotate when the cleaning plate 34 rotates, so that the inner wall of the collecting cylinder 1 is cleaned, labor force is saved, and cleaning efficiency is improved.
The tip fixed connection of pivot 31 and transmission shaft 11, the surface of scraper blade 36 and the fixed connection of surface of bull stick 33, can drive bull stick 33 and rotate when rotating from the rotating ring 32, the one side that scraper blade 36 kept away from bull stick 33 and the inner wall contact of collection section of thick bamboo 1, the one end that cleaning brush 35 kept away from cleaning plate 34 and the inner wall contact of collection section of thick bamboo 1, can drive cleaning plate 34 and rotate when bull stick 33 rotates, thereby carry out quick collection to the protein of collection section of thick bamboo 1 inner wall, not only collect remaining protein effectively and reduced the damage, but also clear up collection section of thick bamboo 1 inner wall through cleaning brush 35, the time of cleaning has been saved, the efficiency has been improved for the next use.
The collecting mechanism 50 comprises a threaded rod 51, at this time, the threaded plate 52 moves to a position where the surface of the threaded rod 51 is not threaded, the threaded rod 51 is driven to rotate when the rotating shaft 31 rotates, one end of the pushing plate 54 away from the threaded plate 52 is rotationally connected with a collecting contraction ring 55, the bottom of the threaded plate 52 is fixedly connected with a limiting spring 56, the bottom of the collecting barrel 1 is provided with a storage box 57, the inner wall of the collecting barrel 1 is fixedly connected with a drainage inclined plate 58, and protein flows downwards through the guidance of the drainage inclined plate 58.
One end of the rotating shaft 31 far away from the transmission shaft 11 is fixedly connected with the end part of the threaded rod 51, the threaded rod 51 keeps static through the limiting spring 56, at this time, the threaded rod 51 keeps rotating, the bottom of the collecting and shrinking ring 55 is contacted with the bottom of the inner wall of the collecting cylinder 1, the outer wall of the scraping ring 53 is contacted with the inner wall of the collecting cylinder 1, meanwhile, the scraping ring 53 moves to the upper part of the drainage sloping plate 58 and keeps static and is not contacted with the drainage sloping plate 58, one end of the threaded rod 51 far away from the rotating shaft 31 is rotationally connected with the inner wall of the collecting cylinder 1, the top of the collecting box 57 is contacted with the bottom of the collecting cylinder 1, the bottom of the collecting box 57 is contacted with the surface of the bottom plate 3, and when the pushing plate 54 moves, the collecting and shrinking ring 55 is pushed to the inner wall of the collecting box 57, so that the residual protein is collected rapidly, and the damage of the protein is reduced.
When in use, firstly, the motor 5 is started to drive the transmission shaft 11 to rotate, the threaded ring 12 is driven to rotate when the transmission shaft 11 rotates, the swivel 13 is driven to rotate when the threaded ring 12 rotates, the stressed inclined plate 17 is extruded when the swivel 13 rotates, the movable plate 16 is pushed to slide downwards on the inner wall of the chute plate 15 when the stressed inclined plate 17 is extruded, the driven push rod 18 is pushed to move in the direction away from each other when the movable plate 16 slides, the contact telescopic rod 19 is pushed to extend towards the direction close to each other when the driven push rod 18 moves and is contacted with the surface of the collecting cylinder 1, the reset spring 20 is extruded when the movable plate 16 slides, the reset spring 20 is contracted downwards when the reset spring 20 is extruded, the movable plate 16 has no extrusion force when the extrusion of the stressed inclined plate 17 is finished when the extrusion bent rod 14 is extruded, the movable plate 16 is reset to the original position through the elasticity of the reset spring 20, simultaneously, the driven push rod 18 pulls the contact telescopic rod 19 to return to the original position, the transmission shaft 11 drives the threaded ring 12 to rotate, the rotating ring 13 drives the extrusion bent rods 14 to rotate and extrudes the stressed inclined plates 17, the extrusion bent rods 14 are arranged in a plurality, and each extrusion bent rod 14 is separated by a certain distance, the stressed inclined plates 17 are extruded, so that the stressed inclined plates 17 push the moving plate 16 to slide reciprocally, the driven push rod 18 is pushed to slide reciprocally when the moving plate 16 slides reciprocally, the driven push rod 18 pushes the contact telescopic rod 19 to extend reciprocally and lightly impact the surface of the collecting cylinder 1, so that the inner wall of the collecting cylinder 1 generates vibration sensation, the rotating shaft 31 is driven to rotate when the transmission shaft 11 rotates, the driven ring 32 is driven to rotate when the rotating shaft 31 rotates, the rotating rod 33 is driven to rotate when the driven ring 32 rotates, when the rotating rod 33 rotates, the cleaning plate 34 is driven to rotate, the cleaning brush 35 is driven to rotate when the cleaning plate 34 rotates, so that the inner wall of the collecting cylinder 1 is cleaned, labor force is saved, cleaning efficiency is improved, when the bottom plate 3 rotates, the scraping plate 36 is driven to rotate, the inner wall of the collecting cylinder 1 is scraped when the scraping plate 36 rotates, residual protein on the inner wall of the collecting cylinder 1 is scraped to the inner wall of the guide groove through rotating inertia when the scraping plate 36 rotates, the protein flows downwards along the guide groove when flowing to the guide groove, and the protein on the inner wall of the guide groove flows downwards rapidly by being matched with vibration feeling generated by impact of the contact telescopic rod 19 on the surface of the collecting cylinder 1, so that the protein on the inner wall of the collecting cylinder 1 is rapidly collected, when the rotating shaft 31 rotates, the threaded rod 51 is driven to rotate, when the threaded rod 51 rotates, the threaded plate 52 moves downwards, the scraping ring 53 is pulled to move downwards when the threaded plate 52 moves, the lower surface of the inner wall of the collecting cylinder 1 is scraped downwards when the scraping ring 53 moves, protein on the inner wall is scraped downwards, the protein flows downwards through the guide of the drainage inclined plate 58, at the moment, the threaded plate 52 moves to a position where the surface of the threaded rod 51 is not threaded, the limiting spring 56 limits the threaded plate 52 to keep static, at the moment, the threaded rod 51 keeps rotating, meanwhile, the scraping ring 53 moves to the upper side of the drainage inclined plate 58 to keep static and not contact with the drainage inclined plate 58, the pushing plate 54 is pushed to move downwards when the scraping ring 53 moves downwards, and the collecting and shrinking ring 55 is pushed to shrink towards each other when the pushing plate 54 moves.
The preferred embodiments of the invention disclosed above are intended only to assist in the explanation of the invention. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise form disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best understand and utilize the invention. The invention is limited only by the claims and the full scope and equivalents thereof.
Claims (8)
1. The utility model provides a stem cell differentiation produces protein collection device of secretion based on precipitation method, includes collection tube (1), the fixed surface of collection tube (1) is connected with supporting legs (2), the bottom fixedly connected with bottom plate (3) of supporting legs (2), the fixed surface of bottom plate (3) is connected with support frame (4), the top fixedly connected with motor (5) of support frame (4), its characterized in that still includes;
The vibration mechanism (10), the vibration mechanism (10) comprises a moving plate (16), a driven push rod (18) is rotatably connected to the surface of the moving plate (16), and a contact telescopic rod (19) is rotatably connected to one end, far away from the moving plate (16), of the driven push rod (18);
The scraping mechanism (30), the scraping mechanism (30) comprises a scraping plate (36), a guide groove is formed in the surface of the scraping plate (36), and a guide groove plate (37) is fixedly connected to the bottom of the scraping plate (36);
The collecting mechanism (50), the collecting mechanism (50) comprises a threaded plate (52), scraping rings (53) are fixedly connected to the two ends of the threaded plate (52), and a pushing plate (54) is rotatably connected to the bottom of the threaded plate (52).
2. The device for collecting secreted proteins generated by stem cell differentiation based on the precipitation method according to claim 1, wherein the number of the supporting frames (4) is four, the four supporting frames (4) are symmetrically arranged by taking the bottom plate (3) as a center, the bottom of the inner wall of the collecting cylinder (1) is provided with a containing hole, and the number of the supporting legs (2) is three.
3. The device for collecting secreted proteins by stem cell differentiation based on the precipitation method according to claim 2, wherein the vibration mechanism (10) comprises a transmission shaft (11), a threaded ring (12) is fixedly connected to the surface of the transmission shaft (11), a rotating ring (13) is fixedly connected to the surface of the threaded ring (12), an extrusion bent rod (14) is fixedly connected to the bottom of the rotating ring (13), a chute plate (15) is fixedly connected to the inner wall of the supporting frame (4), a stressed inclined plate (17) is fixedly connected to the top of the moving plate (16), and a return spring (20) is fixedly connected to the bottom of the moving plate (16).
4. The device for collecting secreted proteins produced by stem cell differentiation based on precipitation method according to claim 3, wherein the end of the transmission shaft (11) is fixedly connected with the output end of the motor (5), the two ends of the moving plate (16) are slidably connected with the inner wall of the sliding groove plate (15), the end of the extrusion bent rod (14) far away from the swivel (13) is contacted with the surface of the stressed inclined plate (17), and the end of the contact telescopic rod (19) far away from the driven push rod (18) is fixedly connected with the inner wall of the supporting frame (4).
5. The device for collecting secreted proteins produced by stem cell differentiation based on the precipitation method of claim 4, wherein the scraping mechanism (30) comprises a rotating shaft (31), a driven ring (32) is fixedly connected to the surface of the rotating shaft (31), a rotating rod (33) is fixedly connected to the surface of the driven ring (32), a cleaning plate (34) is fixedly connected to one end, far away from the driven ring (32), of the rotating rod (33), and a cleaning brush (35) is fixedly connected to the surface of the cleaning plate (34).
6. The device for collecting secreted proteins produced by stem cell differentiation based on precipitation method according to claim 5, wherein the end of the rotating shaft (31) is fixedly connected with the end of the transmission shaft (11), the surface of the scraper (36) is fixedly connected with the surface of the rotating rod (33), one surface of the scraper (36) far away from the rotating rod (33) is contacted with the inner wall of the collecting cylinder (1), and one end of the cleaning brush (35) far away from the cleaning plate (34) is contacted with the inner wall of the collecting cylinder (1).
7. The device for collecting secreted proteins produced by stem cell differentiation based on the precipitation method of claim 6, wherein the collecting mechanism (50) comprises a threaded rod (51), one end of the pushing plate (54) far away from the threaded plate (52) is rotationally connected with a collecting contraction ring (55), the bottom of the threaded plate (52) is fixedly connected with a limiting spring (56), the bottom of the collecting cylinder (1) is provided with a storage box (57), and the inner wall of the collecting cylinder (1) is fixedly connected with a drainage inclined plate (58).
8. The device for collecting secreted proteins produced by stem cell differentiation based on the precipitation method according to claim 7, wherein one end of the rotating shaft (31) far from the transmission shaft (11) is fixedly connected with the end part of the threaded rod (51), the bottom part of the collecting and shrinking ring (55) is contacted with the bottom part of the inner wall of the collecting cylinder (1), the outer wall of the scraping ring (53) is contacted with the inner wall of the collecting cylinder (1), one end of the threaded rod (51) far from the rotating shaft (31) is rotatably connected with the inner wall of the collecting cylinder (1), the top part of the containing box (57) is contacted with the bottom part of the collecting cylinder (1), and the bottom part of the containing box (57) is contacted with the surface of the bottom plate (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202510460543.XA CN120285630B (en) | 2025-04-14 | 2025-04-14 | A protein collection device based on precipitation method for stem cell differentiation and secretion |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202510460543.XA CN120285630B (en) | 2025-04-14 | 2025-04-14 | A protein collection device based on precipitation method for stem cell differentiation and secretion |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN120285630A true CN120285630A (en) | 2025-07-11 |
| CN120285630B CN120285630B (en) | 2025-11-07 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN202510460543.XA Active CN120285630B (en) | 2025-04-14 | 2025-04-14 | A protein collection device based on precipitation method for stem cell differentiation and secretion |
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| US20100051520A1 (en) * | 2007-04-17 | 2010-03-04 | Marks Neil H | Protein skimmer |
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| WO2022041738A1 (en) * | 2021-04-08 | 2022-03-03 | 昆山梓澜电子材料有限公司 | Automatic pulp adding device of coating machine for fluorine release film production |
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| CN218811466U (en) * | 2022-12-23 | 2023-04-07 | 武汉原谷生物科技有限责任公司 | A device that is used for bovine serum albumin to salt out and collects |
| CN117645339A (en) * | 2024-01-23 | 2024-03-05 | 利得环境科技（北京）有限公司 | Sludge separation device for coal chemical wastewater treatment |
| CN118416534A (en) * | 2024-07-04 | 2024-08-02 | 无锡五谷食代科技有限公司 | Equipment and method for pretreatment of protein extraction from cereals and grains |
| CN118987768A (en) * | 2024-10-22 | 2024-11-22 | 江苏顺骁工程科技有限公司 | Waste water treatment equipment for hydraulic engineering |
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2025
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Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US20100051520A1 (en) * | 2007-04-17 | 2010-03-04 | Marks Neil H | Protein skimmer |
| WO2010074953A1 (en) * | 2008-12-16 | 2010-07-01 | Millipore Corporation | Stirred tank reactor and method |
| CN208049740U (en) * | 2018-02-10 | 2018-11-06 | 山西同天翔有色金属有限公司 | A kind of coating production agitating device |
| WO2022041738A1 (en) * | 2021-04-08 | 2022-03-03 | 昆山梓澜电子材料有限公司 | Automatic pulp adding device of coating machine for fluorine release film production |
| CN113786635A (en) * | 2021-08-19 | 2021-12-14 | 闵可可 | Collagen peptide drying system |
| CN115215465A (en) * | 2022-06-01 | 2022-10-21 | 台州耘智科技有限公司 | Domestic sewage treatment flocculation device and treatment method thereof |
| CN218811466U (en) * | 2022-12-23 | 2023-04-07 | 武汉原谷生物科技有限责任公司 | A device that is used for bovine serum albumin to salt out and collects |
| CN117645339A (en) * | 2024-01-23 | 2024-03-05 | 利得环境科技（北京）有限公司 | Sludge separation device for coal chemical wastewater treatment |
| CN118416534A (en) * | 2024-07-04 | 2024-08-02 | 无锡五谷食代科技有限公司 | Equipment and method for pretreatment of protein extraction from cereals and grains |
| CN118987768A (en) * | 2024-10-22 | 2024-11-22 | 江苏顺骁工程科技有限公司 | Waste water treatment equipment for hydraulic engineering |
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| CN120285630B (en) | 2025-11-07 |
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