CN120005732B - A type of Oriental long-bridged fungus and its artificial cultivation method - Google Patents
A type of Oriental long-bridged fungus and its artificial cultivation methodInfo
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Abstract
The invention belongs to the technical field of edible fungi, and particularly relates to a small-long-bridge orientalis and an artificial cultivation method thereof. The invention obtains a strain of wild armillariella maindroni (Ponticulomyces orientalis) named as Kaiyou mushroom CS12 through collection, separation, identification and pure culture of wild mushrooms, and the strain is preserved in China Center for Type Culture Collection (CCTCC) No. M20242547. The domestication cultivation method of the Kaiyoumo CS12 is formed by the strain propagation, bag cultivation and fruiting light, temperature, water and gas condition control. The bacterial age of the Kaiyou mushroom CS12 is 40-85 days, and the bioconversion rate is 66.37% -120.23%. The Kaiyou mushroom CS12 fruiting body is rich in nutrients such as polysaccharide, protein, amino acid and the like, delicious in taste, rich in nutrition, crisp and tender in taste of the stipe, thin and smooth in fungus cover, fresh in taste, thick in fragrance and good in taste.
Description
Technical Field
The invention belongs to the technical field of edible fungi, and particularly relates to a small-long-bridge orientalis and an artificial cultivation method thereof.
Background
Ponticulomyces is a new genus proposed in recent years in Oudemansiella/Xerula, which contains two species, ponticulommyces kedrovayae from the far east of russia and Ponticulumyces orientalis from china (Oudemansilla orientalis Zhu l. Yang), based on morphological and molecular data. The main characteristic of the genus is that the fungus cover is sticky, the fruiting body grows on the rotten wood, and there are two types of capsules. The fungus Ponticulomyces orientalis (Oudemansilla orientalis Zhul. Yang) is one of 966 classification units of domestic edible fungi, but no related report of artificial cultivation is found at home and abroad at present.
Disclosure of Invention
The invention discloses a cultivation method for a Kaiyou mushroom CS12 strain, which comprises the steps of collecting wild sporophores by researchers, separating, identifying, pure culturing and preserving the strain to obtain the Kaiyou mushroom CS12 strain, and performing solid and liquid propagation, bag material cultivation, fruiting light, temperature, water, gas condition control and the like on the strain to form the Kaiyou mushroom CS12 strain, and is realized by the following technical scheme:
the Oriental longbridge is obtained by collecting, identifying, separating and pure culturing wild fungus, named Kaiyou mushroom CS12, and has been preserved in China Center for Type Culture Collection (CCTCC) No. M20242547, and the preservation date is 2024, 11 months and 13 days.
The method for obtaining the eastern bridgegrass comprises the following steps of collecting a wild fungus strain from a broad-leaved stump of southeast of Guizhou Qian, sterilizing by using 1%HgCl 2, culturing tissue blocks with the thickness of 1-3mm 3 on a PDA culture medium flat plate at 25 ℃, and selecting colony edge hypha for transfer culture when the hypha grows to 2/3 area of the flat plate, so as to obtain a pure culture strain with the number of CS12. The PDA culture medium consists of 200g of potato, 20g of glucose, 15g of agar and 1000ml of water.
The strain identification comprises clustered or single fruiting bodies of the strain, 20-70mm wide fungus cover, hemispherical to conical bulges, the color of the fruiting bodies is brown to dark brown when the fruiting bodies are immature, the color of the fruiting bodies is dark in the middle of the fruiting bodies from smoke gray to mouse gray when the fruiting bodies are mature, the edges of the fruiting bodies are light, the fungus folds are radial, and the fruiting bodies are thin, short, soft and fluffy (young) to slightly fluffy and sticky when the fruiting bodies are wet. The fungus meat is white. The fungus folds are white, curved and raw, thin and have no edge. The stipe is 30-300×5-40mm, from top to bottom, grey-white to brown, with white small scale, no ring and no root. 18S rDNA-ITS sequence analysis and alignment in NCBI database (blast. NCBI. Lm. Nih. Gov), the classification name was determined as Botrytis cinerea (Ponticulomyces orientalis), named Kaplan mushroom CS 12.
The 18S rDNA-ITS sequence analysis was:
TTTGACGCTTGTGGCTTCACTTCTGTTGCTGACTTTCCTTAGGGGAAGTATGTGCACGTTGGGAAGTCGCTCGCCTCTTCTTTGTCCACCTGTGCACCTTTTGTAGATCTGGTTGGGAAGCTCACTTGAACGTTAACTCGTTCAAGTGGATTTTGAAGGGTTTGCTTCGGTGCTCCCTTTGTCCGCCAGGTCTATGCTTCATATCATCTCTTTGTATGTTTAGAATGTCTCGTTTATTGGACTTTGTCCATTAACAAACCTAATACAACTTTCAACAACGGATCTCTTGGCTCTCGCATCGATGAAGAACGCAGCGAAATGCGATAACTAATGTGAATTGCAGAATTCAGTGAATCATCGAGTCTTTGAACGCACCTTGCGCCCTTTGGTATTCCGAAGGGGCATGCCTGTTTGAGTGTCAGTAAATTTCTCAACCCTCCTTTACTTTGTTGTTAAAGGATTGGCGTTGGGATGGGTGGAGGCTTGCCGGACGTCAACGTTCGGCTCCTCTGAAATGCATTAGCAGTACAACCATTTACTTGGGCTTCGCTAAGCTGTGATAATATCTAAGCTAGCTTGGTTCAGAGTGTTGGCAGAGCTCGGGGTTTTTTGAAGGGTTTTTGCTTCGCGGCTCCCTTTGCGTTCTCTCTTCTCGAGAGATACCTATGCGACTCTGTGCACGGTTTTTGTTACCGCTTCGAACCGTCTTCTTCACTGAGACAACCTTAACTGACTA-TTGACCTCAAATCAGGTAGACTAC
The artificial cultivation method of the armyworm comprises the following steps:
1. Preparing a mother:
selecting preserved strain, inoculating bacterial blocks with diameter of 5mm to a culture medium plate or test tube under aseptic condition, and culturing at 25deg.C until mycelia overgrow the surface of the culture medium. The culture medium is prepared from the following raw materials of 200g/L (boiled liquid) of potato, 20g/L of glucose, 15g/L of agar, 2-15g/L of peptone, 1-10g/L of yeast powder and natural pH.
2. Original seed preparation:
(1) Preparation of liquid stock
The stock liquid culture medium (g/L) comprises soybean powder 10-100, corn powder 10-40, rapeseed cake powder 10-50, KH 2PO41-5,MgSO4 -5, peptone 5-20, yeast powder 1-10, and pH5.5-7.5. Accurately weighing the above materials according to the formula proportion to prepare a culture medium, sterilizing at 121 ℃ for 30min, selecting a mother seed flat plate or test tube with white, dense, robust and pollution-free hypha, picking 2-6 pieces of hypha with the diameter of 5mm and vigorous growth under the aseptic condition, inoculating into a triangular flask with the liquid loading capacity of 35-1500ml and the liquid loading capacity of 35-1500ml, standing for 12-36h after inoculating, and culturing on a shaking table with the rotating speed of 25 ℃ and 100-300r/min for 5-12 days.
(2) Preparation of solid stock
The stock solid culture medium comprises 40-80% of wood dust, 1-10% of corn powder, 10-30% of chaff, 10-30% of cotton seed hulls, 10-35% of wheat bran, 1-5% of sucrose, 1-2% of lime, 1-2% of gypsum and natural pH. Accurately weighing the above materials or all materials according to the formula proportion to prepare a culture medium, stirring uniformly, adding water to a water content of 55-65%, filling into a polypropylene plastic bag with a length of 8cm multiplied by 28cm multiplied by 0.005cm, sealing by a sleeve ring, sterilizing the filled stock bag in a high-pressure steam sterilizing pot for 2-4 h at 121 ℃, taking out when the temperature of the culture bag is reduced to 60-70 ℃, cooling to 28 ℃, and selecting a flat plate with white, thick, strong and pollution-free mycelium. Under aseptic condition, 2-6 pieces of mycelium with diameter of 1cm and vigorous growth are selected and inoculated into a fungus bag, and the fungus bag is placed in a 25 ℃ incubator for light-proof culture until the bag is full.
3. Preparing cultivated species:
(1) Liquid cultivar preparation
The culture medium for liquid culture of cultivated species (g/L) comprises 20-200 parts of wood powder (100 meshes), 50-200 parts of bran, 10-100 parts of soybean powder, 5-100 parts of glucose, 10-40 parts of corn flour, 1-10 parts of peptone, 1-5 parts of potassium dihydrogen phosphate, 0.2-2 parts of magnesium sulfate, 0.1-2 parts of dichlord, 10.002-0.5 parts of VB10 and 5.5-7.5 parts of pH. Accurately weighing the above materials or all materials according to the formula proportion to prepare a culture medium, adding the culture medium according to the liquid loading amount of 1/2-2/3 of the fermentation tank, sterilizing at 121 ℃ for 30min, cooling to 25 ℃, inoculating a liquid stock according to the inoculation amount of 5-20% of the volume of the culture medium in the fermentation tank, and culturing for 5-20d. The culture condition is that the ventilation is 20-40L/1.0m 3/h, the tank pressure is 0.03MPa, and the culture temperature is 25 ℃.
(2) Preparation of solid cultivars
The culture medium is composed of wood dust 40-80%, corn powder 1-10%, husk 10-30%, cotton seed husk 10-30%, wheat bran 10-35%, sucrose 1-5%, lime 1-2%, gypsum 1-2%, and natural pH. Accurately weighing the above materials according to the formula proportion to prepare a culture medium, stirring uniformly, adding water to a water content of 55-65%, filling into a polypropylene plastic bag with a volume of 8cm multiplied by 28cm multiplied by 0.005cm, sealing by a lantern ring, sterilizing the filled stock bag in a high-pressure steam sterilizing pot for 2-4 h at 121 ℃, taking out when the temperature of the cultivation bag is reduced to 60-70 ℃, cooling to 28 ℃, inoculating 15-20ml of liquid stock or 1-10g of solid stock, and culturing in a dark place at 25 ℃ until the bag is full.
4. Preparing a cultivation fungus bag:
The culture materials comprise 20-80% of wood dust, 10-40% of oil tea shell (powder), 20-90% of cotton seed shell, 1-20% of bran, 1-5% of sucrose, 1-3% of lime, 1-3% of gypsum and natural pH.
Accurately weighing the above materials according to the formula proportion, stirring, adding water to water content of 55-65%, packaging into polypropylene plastic bag with 8cm×28cm×0.005cm, sealing with collar, sterilizing the packaged cultivation bag in high pressure steam sterilizing pot at 121deg.C for 2-4 hr, taking out when the cultivation bag temperature is reduced to 60-70deg.C, cooling to 28deg.C, inoculating 20-35ml liquid strain or 5-10g solid strain, and culturing in the dark at 25deg.C until the bag is full.
4. Fruiting management:
(1) Cultivation without soil covering
And (3) transferring the fungus bags reaching physiological maturity to a mushroom house, opening a bag opening, putting the fungus bags on a bedstead in order, keeping the relative humidity of room air above 90%, illuminating by 100-500lx, controlling the temperature at 15-25 ℃, taking care of ventilation, and enabling primordia to appear in 10-20 days. Harvesting when fruiting body grows until the fungus cover opens 40-60%.
(2) Soil-covered cultivation
And (3) transferring the fungus bags which reach physiological maturity into a mushroom house layer frame, cutting off an upper plastic bag film along the upper edge of the bag material, opening 2-3 small openings at two sides of the fungus bags to prevent the fungus bags from accumulating water, and covering soil between the fungus bags and on the fungus bags to a high discharge surface of 2-4cm. And (5) after earthing, watering thoroughly. Primordia can appear in 10-25 days. Harvesting when fruiting body grows until the fungus cover opens 40-60%.
The invention has the beneficial effects that:
The invention obtains a strain of wild armillaria orientalis, named as Kaiyoumo CS12, through wild fungus collection, separation, identification and pure culture. The artificial cultivation method of the Kaiyoumo CS12 strain is formed through researches of solid and liquid propagation of the strain, bag material cultivation, fruiting light, temperature, water and gas condition control and the like. The invention can realize the commercial cultivation of the Kaiyou mushroom CS12, adds new types of edible fungus markets, and simultaneously provides an artificial cultivation method of wild edible strains. The bacterial age of the Kaiyou mushroom CS12 is 40-85 days, and the bioconversion rate is 66.37% -120.23%. The Kaiyou mushroom CS12 fruiting body is rich in nutrients such as polysaccharide, protein, amino acid and the like, delicious in taste, rich in nutrition, crisp and tender in taste of the stipe, thin and smooth in fungus cover, fresh in taste, thick in fragrance and good in taste.
Drawings
FIG. 1 is a flow chart of artificial cultivation of Alternaria orientalis;
FIG. 2 is a photograph of the harvested Oriental longbridge fungus of example 1;
FIG. 3 is a phylogenetic tree diagram.
Detailed Description
The technical scheme of the present invention is further defined below in conjunction with the specific embodiments, but the scope of the claimed invention is not limited to the description.
Example 1
1. Preparation of mother seeds
The culture medium (g/L) comprises potato 200 (decoction), glucose 20, peptone 2, yeast powder 2, agar 15, and natural pH. Selecting preserved mother seeds, picking fungus blocks with the diameter of 5mm under the aseptic condition, inoculating the fungus blocks into the culture medium plate, culturing the fungus blocks after inoculating the fungus blocks at 25 ℃, and enabling mycelia to grow on the surface of the culture medium after 7 days.
2. Preparation of stock
The solid culture medium comprises 40% of wood dust, 2% of corn powder, 30% of cotton seed hulls, 10% of rice hulls, 15% of wheat bran, 1% of sucrose, 1% of lime, 1% of gypsum and natural pH.
Accurately weighing materials according to the formula proportion, adding water to the water content of 65% after uniformly stirring, filling the materials into a polypropylene plastic bag with the water content of 8cm multiplied by 28cm multiplied by 0.005cm, sealing the bag by a lantern ring, sterilizing the filled cultivation bag for 2 hours at the temperature of 121 ℃ in a high-pressure steam sterilizing pot, taking out the cultivation bag when the temperature of the cultivation bag is reduced to 60-70 ℃, cooling the cultivation bag to 28 ℃, selecting a mother seed flat plate with white, dense, strong and pollution-free mycelium, taking 5 fungus blocks by a puncher with the diameter of 1cm, inoculating the fungus blocks into a fungus bag, and placing the fungus bag in a 26 ℃ incubator for light-shielding cultivation until the fungus bag is full of the fungus blocks.
3. Preparation of cultivars
The solid culture medium comprises 40% of wood dust, 2% of corn powder, 30% of cotton seed hulls, 10% of rice hulls, 15% of wheat bran, 1% of sucrose, 1% of lime, 1% of gypsum and natural pH.
Accurately weighing materials according to the formula proportion, stirring uniformly, adding water to 65% of water content, filling into a polypropylene plastic bag with the volume of 8cm multiplied by 28cm multiplied by 0.005cm, sealing by a lantern ring, sterilizing the filled cultivation bag for 2 hours at the temperature of 121 ℃ in a high-pressure steam sterilizing pot, taking out when the temperature of the cultivation bag is reduced to 60-70 ℃, cooling to 28 ℃, inoculating 5g of solid stock, and culturing in the dark at 25 ℃ until the cultivation bag is full.
4. Preparation of cultivation fungus bag
33% Of wood dust, 50% of cotton seed hulls, 15% of bran, 1% of lime, 1% of gypsum and natural pH.
Accurately weighing materials according to the formula proportion, adding water to water content of 65%, placing into polypropylene plastic bag with 8cm×28cm×0.005cm, sealing with collar, sterilizing the packaged cultivation bag in high pressure steam sterilizing pot at 121deg.C for 4 hr, taking out when the cultivation bag temperature is reduced to 60-70deg.C, cooling to 28deg.C, and inoculating about 5g solid strain. Placed in a light-protected culture at 25℃and packed in 42d bags.
5. Fruiting management
And (3) transferring the fungus bags reaching physiological maturity to a mushroom house, opening a bag opening, putting the fungus bags on a bedstead in order, keeping the relative humidity of room air to be more than 90%, illuminating by 200-300lx, controlling the temperature at 18 ℃, ventilating, and generating primordia in 16 days. Harvesting when fruiting body grows until the fungus cover opens 40-60%. The bioconversion rate is 103.25%.
Example 2
1. Preparation of mother seeds
The culture medium (g/L) is potato 200 (boiled liquid), glucose 20, agar 15, and natural pH. Selecting preserved mother seeds, picking fungus blocks with the diameter of 5mm under the aseptic condition, inoculating the fungus blocks into the culture medium plate, culturing the fungus blocks after inoculating the fungus blocks at 25 ℃, and enabling mycelia to grow on the surface of the culture medium after 7 days.
2. Preparation of stock
The liquid culture medium (g/L) comprises soybean powder 10, corn powder 20, KH 2PO41,MgSO4, peptone 5, and pH6.5. Sterilizing at 121deg.C for 30min after adding proportionally.
Accurately weighing the above materials or all materials according to the formula proportion to prepare a culture medium, sterilizing at 121 ℃ for 30min, taking 5 mycelia white, dense, robust and pollution-free mother strain flat fungus blocks by a puncher with the diameter of 5mm under the aseptic condition, inoculating the mycelia white, dense, robust and pollution-free flat fungus blocks into a 250ml triangular flask, and filling the culture medium with the liquid volume of 90ml. After inoculation, the mixture is allowed to stand for 12 hours and then is placed on a shaking table at 25 ℃ and 180r/min for 7 days.
3. Preparation of cultivars
The liquid culture medium (g/L) comprises wood powder (100 meshes) 20, corn powder 10, KH 2PO41,MgSO4, peptone 3, dichlord 0.5 and pH6.5.
A50L fermenter was charged with 30L of the above medium and sterilized at 121℃for 30min. The liquid stock was inoculated in an inoculum size of 10% of the volume of the medium and cultured for 10 days. The culture condition is that the ventilation is 1.0m 3/h, the tank pressure is 0.03MPa, and the culture temperature is 25 ℃.
4. Preparation of cultivation fungus bag
33% Of wood dust, 50% of cotton seed hulls, 15% of bran, 1% of lime, 1% of gypsum and natural pH.
Accurately weighing materials according to the formula proportion, adding water to water content of 65%, placing into polypropylene plastic bags with 8cm×28cm×0.005cm, sealing with collar, sterilizing the packaged cultivation bags in a high pressure steam sterilizing pot at 121deg.C for 4 hr, taking out when the cultivation bag temperature is reduced to 60-70deg.C, cooling to 28deg.C, and inoculating 15ml liquid strain per bag. Placed in a light-protected culture at 25℃and filled with 51d bags.
5. Fruiting management
And (3) transferring the fungus bags reaching physiological maturity to a mushroom house, opening a bag opening, putting the fungus bags on a bedstead in order, keeping the relative humidity of room air to be more than 90%, illuminating by 200-300lx, controlling the temperature at 20 ℃, ventilating, and generating primordia in 16 days. Harvesting when fruiting body grows until the fungus cover opens 40-60%. The bioconversion rate was 66.37%.
Physical form of acclimatized fruit body
The width of the cap of Kaiyou mushroom CS12 is 20-60mm, the cap is hemispherical to conical, the color of the fruiting body is brown to dark brown when the fruiting body is immature, the color of the fruiting body is dark in the middle of the fruiting body from smoke gray to mouse gray when the fruiting body is mature, the edge of the fruiting body is light, the fruiting body is radial, and the fruiting body is thin, short, soft and fluffy (young) to slightly fluffy and sticky when the fruiting body is wet. The fungus meat is white. The fungus folds are white, curved and raw, thin and have no edge. The stipe is 30-300×5-40mm, from top to bottom, grey-white to brown, with white small scale, no ring and no root. The bioconversion rate is 66.37% -120.23%.
The results of the measurement of the nutrient components of the longbridge eastern cultivated by the method of example 1 are shown in Table 1.
Table 1 example 1 determination of the content of nutritional ingredients related to fresh fruit bodies
It is noted that the above embodiments and the comparative examples are only limited by the further explanation and understanding of the technical solutions of the present invention, and are not to be construed as further limiting the technical solutions of the present invention, and the invention without significant essential features and significant improvements made by those skilled in the art still falls within the protection scope of the present invention.
Claims (1)
1. The artificial cultivation method of the longbridge orientalis is characterized by comprising the following steps of:
(1) Preparing a mother:
Selecting preserved strain, inoculating a strain block with a diameter of 5mm into a culture medium flat plate or a test tube under a sterile condition, and culturing at 25 ℃ until hypha grows on the surface of the culture medium, wherein the culture medium is prepared from the following raw materials of 200g/L of potato boiling liquid, 20g/L of glucose, 15g/L of agar, 2-15g/L of peptone, 1-10g/L of yeast powder and natural pH;
(2) Original seed preparation:
(a) Preparation of liquid stock
The stock liquid culture medium (g/L) comprises 10-100 parts of soybean powder, 10-40 parts of corn powder, 10-50 parts of rapeseed cake powder, 1-5 parts of KH 2PO4 1-5,MgSO4, 5-20 parts of peptone, 1-10 parts of yeast powder and 5.5-7.5 parts of pH, accurately weighing the above materials according to the formula proportion to prepare a culture medium, sterilizing at 121 ℃ for 30min, selecting a stock flat plate or test tube with white, dense, robust and pollution-free mycelium, picking 2-6 blocks of mycelium with the diameter of 5mm under aseptic condition, inoculating into a triangular flask with the diameter of 100-5000ml, placing the liquid volume of 35-1500ml into a static stand for 12-36h after inoculation, and culturing on a shaking table with the rotation speed of 100-300r/min at 25 ℃ for 5-12 days;
(b) Preparation of solid stock
The solid culture medium for stock seed is prepared with wood chip 40-80 wt%, corn powder 1-10 wt%, rice husk 10-30 wt%, cotton seed husk 10-30 wt%, wheat bran 10-35 wt%, cane sugar 1-5 wt%, lime 1-2 wt%, gypsum 1-2 wt% and natural pH, and through mixing, adding water to water content 55-65 wt%, packing in polypropylene plastic bag of 8 cm X28 cm X0.005 cm, sealing with collar, sterilizing in high pressure steam sterilizing pot at 121 deg.c for 2-4 hr, cooling to 28 deg.c, selecting white, dense and pollution-free flat plate, inoculating 2-6 blocks of 1cm thick mycelium into the bag, and culturing in light-shielding culture box at 25 deg.c.
(3) Preparing cultivated species:
(a) Liquid cultivar preparation
The culture medium is prepared from wood powder (100 mesh 20-200), bran (50-200), soybean powder (10-100), glucose (5-100), corn flour (10-40), peptone (1-10), potassium dihydrogen phosphate (1-5), magnesium sulfate (0.2-2), dichlord (0.1-2), VB10.002-0.5 and pH (5.5-7.5), preparing culture medium by accurately weighing the above materials according to the formula proportion, adding the culture medium according to the liquid loading amount of a fermentation tank (1/2-2/3), sterilizing at 121 ℃ for 30min, cooling to 25 ℃, inoculating liquid stock according to the inoculum size of 5-20% of the volume of the culture medium in the fermentation tank, culturing for 5-20d, wherein the culture condition is that the aeration amount is 20-40L/1.0m 3/h, the tank pressure is 0.03MPa, and the culture temperature is 25 ℃;
(b) Preparation of solid cultivars
The cultivation seed solid culture medium comprises 40-80% of wood dust, 1-10% of corn powder, 10-30% of husk, 10-30% of cotton seed hulls, 10-35% of wheat bran, 1-5% of sucrose, 1-2% of lime and 1-2% of gypsum, and natural pH, wherein the culture medium is prepared by accurately weighing the above materials according to the formula proportion, stirring uniformly, adding water to a polypropylene plastic bag with the water content of 55-65%, filling the bag into a polypropylene plastic bag with the water content of 8 cm X28 cm X0.005 cm, sealing the bag with a lantern ring, sterilizing the filled stock bag in a high-pressure steam sterilizing pot for 2-4 hours at 121 ℃, taking out the bag when the temperature of the bag is reduced to 60-70 ℃, cooling to 28 ℃, inoculating 15-20ml of liquid stock or 1-10g of solid stock, and culturing the bag in a dark place at 25 ℃ until the bag is full;
(4) Preparing a cultivation fungus bag:
the culture materials comprise 20-80% of wood dust, 10-40% of camellia oleifera shell powder, 20-90% of cotton seed hulls, 1-20% of bran, 1-5% of sucrose, 1-3% of lime, 1-3% of gypsum and natural pH;
Accurately weighing the above materials according to the formula proportion, stirring, adding water to water content of 55-65%, packaging into 8 cm ×28× 28 cm ×0.005cm polypropylene plastic bag, sealing with collar, sterilizing the packaged cultivation bag in high pressure steam sterilizing pot at 121deg.C for 2-4 hr, taking out when the cultivation bag temperature is reduced to 60-70deg.C, cooling to 28deg.C, inoculating 20-35ml liquid strain or 5-10g solid strain, and culturing at 25deg.C in dark until the bag is full;
(5) Fruiting management:
(a) Cultivation without soil covering
Transferring the fungus bag reaching physiological maturity to a mushroom house, opening a bag opening, putting the fungus bag on a bedstead in order, keeping the relative humidity of room air above 90%, illuminating by 100-500lx, controlling the temperature at 15-25 o ℃, paying attention to ventilation, collecting primordia after 10-20 days, and harvesting when fruiting bodies grow until fungus covers are opened by 40-60%;
(b) Soil-covered cultivation
Transferring the fungus bags reaching physiological maturity into a mushroom house layer frame, cutting off an upper plastic bag film on the upper edge of a bag material, opening 2-3 small openings at two sides of the fungus bags to prevent the fungus bags from accumulating water, covering soil between the fungus bags and on the fungus bags to a height of 2-4cm of a discharging surface, completely watering after the soil is covered, generating primordium after 10-25 days, and harvesting when fruiting bodies grow until fungus covers are opened by 40-60%;
The said Oriental longbridge is obtained by wild fungus collection, separation, identification and pure culture, named Kaiyoumo CS12, and has been preserved in China Center for Type Culture Collection (CCTCC) No. M20242547, and the preservation date is 2024, 11 months and 13 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202411892136.8A CN120005732B (en) | 2024-12-20 | 2024-12-20 | A type of Oriental long-bridged fungus and its artificial cultivation method |
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| 史忠良 ; .食用菌培养料的分类及处理方式.农技服务.2011,第1.1-1.3小节. * |
| 李挺等.深圳市大型真菌资源多样性调查.菌物研究.2023,第2.4.1小节. * |
| 深圳市大型真菌资源多样性调查;李挺等;菌物研究;20231009;第2.4.1小节 * |
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