CN119842483A - Full-automatic cell culture device and application method thereof - Google Patents
Full-automatic cell culture device and application method thereof Download PDFInfo
- Publication number
- CN119842483A CN119842483A CN202510065564.1A CN202510065564A CN119842483A CN 119842483 A CN119842483 A CN 119842483A CN 202510065564 A CN202510065564 A CN 202510065564A CN 119842483 A CN119842483 A CN 119842483A
- Authority
- CN
- China
- Prior art keywords
- culture
- incubator
- groove
- tube
- sealing cover
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Computer Hardware Design (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a full-automatic cell culture device and a use method thereof, which belong to the technical field of cell culture, a sliding plate is supported by the elasticity of a supporting spring and drives a clamping block to be clamped into a clamping groove through the sealing cover, the purpose of locking the sealing cover is realized, the other positions of the culture box are erected by operating the assembly in the same way, a culture test tube is conveniently fed into the culture box, the sliding plate is pulled by a pull rod, the sliding plate drives the clamping block to be separated from the clamping groove, namely, the locking state between the sealing cover and the culture box is released, each culture groove is independently designed, cells in different time periods and different types can be independently cultured and taken and placed, the operation is convenient, all independently designed erected assemblies are integrated in a single culture box, a plurality of culture platforms are not required to be additionally arranged for cell culture, the convenience in carrying and transferring is also realized, and the cross pollution caused by the conduction of a plurality of culture grooves is prevented while the use cost is reduced.
Description
Technical Field
The invention relates to the technical field of cell culture, in particular to a full-automatic cell culture device and a using method thereof.
Background
Cell culture is broadly used for in vitro culture, and is now widely applied to various fields such as biology, medicine, new medicine research and development, and the like, and becomes one of the most important basic science. The cell culture technology can be a simple single cell or a few differentiated multicellular by a large amount of cells, and the full-automatic cell culture is a method for realizing full automation of the cell culture process by utilizing advanced technology, and the development of the technology has important significance for improving the efficiency and the accuracy of cell culture and reducing the error of manual operation.
At present, an automatic cell culture platform is generally adopted for full-automatic cell culture, test tubes with cells are additionally arranged in the culture platform for culture, when the culture platform is opened for taking and placing the test tubes, all the test tubes with cells can be exposed to the outside, so that cross contamination can occur among the cells, when a plurality of culture platforms are independently additionally arranged for cell culture, the use cost is definitely increased, meanwhile, the carrying and the transferring are inconvenient, and the workload is also increased when the test tubes are taken and placed.
Disclosure of Invention
The invention aims to provide a full-automatic cell culture device and a use method thereof, which are used for solving the problems that in the background art, when a culture platform is opened to take and put test tubes, all the test tubes filled with cells are exposed to the outside, so that cross contamination occurs among the cells, when a plurality of culture platforms are independently additionally arranged to culture the cells, the use cost is definitely increased, meanwhile, the carrying and the transferring are inconvenient, and the workload is increased when the test tubes are taken and put.
In order to achieve the above purpose, the present invention provides the following technical solutions:
The utility model provides a full-automatic cell culture device, includes the incubator, a plurality of culture tank has been seted up outward to the incubator, install in the culture tank and erect the subassembly, erect one side fixedly connected with sealed lid that the incubator was kept away from to the subassembly, sealed lid seals the opening of culture tank, install a plurality of culture test tube in the subassembly of erectting, the spout has been seted up respectively to the top and the bottom of sealed lid, sliding connection has locking Assembly in the spout, and the one end that two locking Assembly is close to each other is fixed with the handle respectively, a plurality of draw-in groove has been seted up respectively to the top and the below of incubator outer wall, locking Assembly's tip joint is in the draw-in groove, automatic culture analyzer is installed at the top of incubator, the tip of automatic culture analyzer runs through the intercommunication in a plurality of culture tank, the centre tank has been seted up at the middle part of incubator, automatic culture analyzer's bottom is installed in the centre tank, four recesses have been seted up to the bottom of incubator, install supporting component in four recesses.
As a further scheme of the invention, a plurality of light-transmitting grooves are formed in the outer wall of the incubator, the inner walls of the light-transmitting grooves are transparent, the light-transmitting grooves are positioned between two adjacent culture grooves, and sealing plates are arranged at the openings of the light-transmitting grooves.
As a further scheme of the invention, a collecting bin is arranged below the inside of the incubator, an electromagnetic valve is arranged at the top of the inner wall of the collecting bin, the electromagnetic valve is arranged at the bottom of the inner wall of the incubator in a penetrating way, and a discharge main valve is arranged at the bottom of the inner wall of the collecting bin.
As a further scheme of the invention, the erection assembly comprises a stand column, a transverse plate and a supporting plate are fixedly connected between the stand column and the sealing cover, two limiting partition plates are arranged between the supporting plate and the transverse plate, the limiting partition plates are arranged on the opposite surfaces of the stand column and the sealing cover, a plurality of clamping holes are formed in the transverse plate, a plurality of ball grooves and leakage holes are formed in the supporting plate, the bottom ends of the culture test tubes penetrate through the clamping holes and the limiting partition plates and are positioned in the ball grooves, and the stand column, the transverse plate and the supporting plate are connected in the culture grooves in a sliding mode.
As a further scheme of the invention, the locking assembly comprises a sliding plate, the sliding plate is in sliding connection with the sliding groove, a clamping block is fixedly connected to the sliding plate and is clamped in the clamping groove, one side of the clamping block is in a cambered surface design, the other side of the clamping block is in a plane design, a pull rod is fixed in the sliding plate, the end part of the pull rod is fixed with the handle, a supporting spring is sleeved outside the pull rod, and the supporting spring is fixed between the sealing cover and the inner wall of the sliding plate.
As a further scheme of the invention, the automatic culture analyzer comprises an automatic control module, wherein a fixing column is arranged in the middle of the automatic control module, the fixing column is arranged in a central groove, a clamping sleeve is arranged outside the automatic control module and connected to the top of the incubator, a group of online observers are respectively arranged at the bottom of the automatic control module corresponding to the positions of a plurality of culture grooves, the number of each group of online observers is five, and each online observer is positioned right above a culture test tube.
As a further scheme of the invention, the support assembly comprises a fixed ring, four support rods are fixed on the fixed ring, universal wheels are installed at the bottom ends of the support rods, the top ends of the support rods are slidably connected in the grooves, buffer springs are sleeved outside the support rods and fixed between the bottom of the incubator and the fixed ring, damping springs are fixed at the top ends of the support rods, and the damping springs are installed in the grooves.
A method of using a fully automated cell culture apparatus, the method comprising the steps of:
When full-automatic cell culture is carried out, a culture tube filled with cells is placed in an erection assembly, the bottom end of the culture tube penetrates through a clamping hole and a limiting baffle plate and is positioned in a ball groove, the culture tube is limited through the ball groove and the clamping hole and is supported by the limiting baffle plate, the erection stability of the culture tube is guaranteed, the top end of the culture tube is higher than the clamping hole, a sealing cover is pushed after the placing work of the culture tube is completed, the sealing cover drives the culture tube to move through a transverse plate and a supporting plate, and the culture tube can enter a culture groove in a culture box;
When the clamping blocks in the locking assemblies are contacted with the side wall of the incubator, the clamping blocks can drive the sliding plates to slide into the sliding grooves after being pressed, when the sealing covers are tightly attached to the side wall of the incubator, the sliding plates are supported by the elasticity of the supporting springs, so that the clamping blocks are driven to be clamped into the clamping grooves by the sliding plates, the purpose of locking the sealing covers is achieved, the sealing covers can seal the opening of the incubator, external dust and impurities are prevented from entering the incubator, the erection assemblies are arranged in each incubator, the erection assemblies at other positions of the incubator are operated in the same way, so that the culture tubes are conveniently fed into the incubator, each incubator is independently designed, cells in different time periods and different types can be independently cultivated and taken and placed, and the erection assemblies with all independent designs are integrated into a single incubator to fully-automatically cultivate the cells in the culture tubes;
When cells in the culture test tube are cultured through the incubator, the bottom end of an online observer in the automatic culture analyzer is communicated with the culture tank, each online observer is located right above the culture test tube, the cells in the culture test tube are observed online through the online observer and transmitted to the automatic control module, the automatic culture analyzer can analyze real-time data, when the culture test tube in the incubator is required to be subjected to light transmission treatment, a sealing plate at the opening of the light transmission tank can be opened, and as the inner wall of the light transmission tank is of transparent design, the light transmission tank is located between two adjacent culture tanks, the culture test tube can be observed through the light transmission tank and transmitted, and the sealing plate is clamped into the opening of the light transmission tank to be carried out;
When the culture tube is damaged or overflows liquid, the liquid flows into the electromagnetic valve below along the culture grooves through the leak holes, the liquid is led into the collecting bin after the electromagnetic valve is opened, the collecting bin can collect the liquid in all the culture grooves in a concentrated manner, the liquid can be discharged out of the culture box through the discharge main valve at the bottom of the collecting bin, each electromagnetic valve is independently designed, and the cross contamination caused by the conduction of a plurality of culture grooves is prevented, so that the culture work of cells is influenced;
When the culture device is transferred, the incubator is pushed to enable the universal wheels in the support assembly to roll on the ground, in the moving process of the incubator, the universal wheels can squeeze the support rods, the support rods squeeze the buffer springs through the fixing rings, the buffer springs can provide reverse acting force for the fixing rings after being pressed, the shock absorption effect on the incubator can be achieved, the end parts of the support rods are supported by the damping springs in the grooves, and the stability of the culture test tubes is prevented from being influenced by overlarge amplitude of the incubator;
When taking out the culture tube, through squeezing two handles, make two handles pull the slide through the pull rod respectively, make the slide drive the fixture block break away from the draw-in groove, remove the locking state between sealed lid and the incubator promptly, outside pull handle can take out the culture tube and cultivate the groove, whole erect the subassembly and cultivate the test tube and can shift out the culture groove completely moreover, and then conveniently put the work of getting of culture tube, when tearing down automatic culture analyzer, grip the cutting ferrule and upwards pull, make the cutting ferrule drive the fixed column through automatic control module and slide in the centre tank, automatic control module then drives a plurality of online observers and shifts out the incubator, can pull down automatic culture analyzer, so that the daily cleanness and the maintenance work of device.
Compared with the prior art, the invention has the beneficial effects that:
1. According to the invention, the locking assembly is close to the incubator, when the sealing cover clings to the side wall of the incubator, the sliding plate is supported by the elastic force of the supporting spring, the sliding plate drives the clamping block to be clamped into the clamping groove, the purpose of locking the sealing cover is achieved, the sealing cover can seal the opening of the incubator, external dust and impurities are prevented from entering the incubator, the erection assembly is installed in each incubator, the erection assemblies at other positions of the incubator are operated in the same way, further, the culture test tube is conveniently fed into the incubator, when the culture test tube is taken out, the two handles are extruded, the sliding plate is pulled by the pull rod respectively, the sliding plate drives the clamping block to be separated from the clamping groove, namely, the locking state between the sealing cover and the incubator is relieved, each incubator is independently designed, the independent culture and the taking and placing of cells of different time periods and different types can be carried out, the operation is convenient, all the independently designed erection assemblies are integrated in a single incubator, further, a plurality of culture platforms are not required to be additionally arranged for cell culture, the cell culture and the cell transfer is convenient to carry, and the cross pollution caused by the conduction of the plurality of incubates can be prevented.
2. According to the invention, the culture test tube filled with cells is placed in the erection assembly, the bottom end of the culture test tube penetrates through the clamping hole and the limiting baffle plate and is positioned in the ball groove, the culture test tube is limited through the ball groove and the clamping hole and is supported by the limiting baffle plate, the erection stability of the culture test tube is guaranteed, the top end of the culture test tube is higher than the clamping hole, the culture test tube is convenient to take and place, when the culture test tube is damaged or overflows liquid, the liquid flows into the electromagnetic valve below along the culture groove through the leakage hole, the electromagnetic valve is opened to enable the liquid to be led into the collection bin, so that the collection bin can collect the liquid in all the culture grooves in a concentrated mode, the discharge main valve at the bottom is opened to discharge the culture box, each electromagnetic valve is of independent design, and the cross contamination caused by the conduction of a plurality of culture grooves is prevented, so that the culture operation on cells is affected.
3. Through squeezing two handles, make the slide drive the fixture block and break away from the draw-in groove, outside pull handle can take out the culture tank with cultivateing the test tube, and whole erect the subassembly and cultivate the test tube and can shift out the culture tank completely moreover, and then conveniently put the work of getting to cultivateing the test tube, when tearing down automatic cultivation analysis appearance, the gripping cutting ferrule is and upwards pulling, make the cutting ferrule drive the fixed column through automatic control module and slide in the centre tank, automatic control module then drives a plurality of online observers and shift out the incubator, can pull down automatic cultivation analysis appearance, therefore the device adopts the mode of modularized design can each part decompose the reassemble, so that the daily cleanness and the maintenance work of device, make the universality of the device wide.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed for the description of the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic view of a three-dimensional structure of the present invention;
FIG. 2 is a schematic view of the structure of the invention;
FIG. 3 is a schematic diagram of the connection of the incubator of the present invention to an automated culture analyzer;
FIG. 4 is a schematic view of the structure of the incubator of the present invention;
FIG. 5 is a schematic view of a partial cross section of the incubator of the present invention;
FIG. 6 is a schematic top view in cross section of the incubator of the present invention;
FIG. 7 is a schematic view of a support assembly according to the present invention;
FIG. 8 is an enlarged schematic view of the structure of FIG. 7A according to the present invention;
FIG. 9 is a schematic view of the construction of the erection assembly of the present invention;
FIG. 10 is a schematic cross-sectional view of a locking assembly of the present invention.
In the drawings, the list of components represented by the various numbers is as follows:
1. Incubator 2, culture tank 3, erection component 301, column 302, clamping hole 303, transverse plate 304, limit baffle plate 305, supporting plate 306, ball tank 307, leak hole 4, sealing cover 5, culture test tube 6, chute 7, locking component 701, slide plate 702, clamping block 703, pull rod 704, supporting spring 8, handle 9, clamping groove 10, automatic culture analyzer 101, automatic control module 102, fixed column 103, clamping sleeve 104, on-line observer 11, central groove 12, groove 13, supporting component 131, fixed ring 132, supporting rod 133, universal wheel 134, buffer spring 135, damping spring 14, light transmission groove 15, sealing plate 16, collecting bin 17, electromagnetic valve 18, discharge main valve.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to fig. 1-10, the invention provides a technical scheme that the full-automatic cell culture device comprises an incubator 1, a plurality of culture tanks 2 are arranged outside the incubator 1, an erection component 3 is installed in the culture tanks 2, a sealing cover 4 is fixedly connected to one side of the erection component 3 far away from the incubator 1, the sealing cover 4 seals an opening of the incubator 2, a plurality of culture test tubes 5 are installed in the erection component 3, sliding grooves 6 are respectively arranged at the top and the bottom of the sealing cover 4, locking components 7 are slidably connected in the sliding grooves 6, handles 8 are respectively fixed at one ends, close to each other, of the two locking components 7, a plurality of clamping grooves 9 are respectively arranged above and below the outer wall of the incubator 1, and the end parts of the locking components 7 are clamped in the clamping grooves 9 to be locked. An automatic culture analyzer 10 is arranged at the top of the incubator 1, the end parts of the automatic culture analyzer 10 are communicated in a plurality of culture tanks 2 in a penetrating way, a central tank 11 is arranged in the middle of the incubator 1, the bottom of the automatic culture analyzer 10 is arranged in the central tank 11, four grooves 12 are arranged at the bottom of the incubator 1, and a supporting component 13 is arranged in each of the four grooves 12;
During operation, every culture tank 2 is independent design, can independently cultivate and get the putting to different time slots and different grade type cells, and the simple operation erects subassembly 3 integration in single incubator 1 with all independent designs, and then need not to add a plurality of cultivation platforms and carry the transfer moreover conveniently, prevents that a plurality of culture tanks 2 from switching on and causing cross contamination.
As a further scheme of the invention, a plurality of transparent grooves 14 are formed in the outer wall of the incubator 1, the inner walls of the transparent grooves 14 are transparent, the transparent grooves 14 are positioned between two adjacent culture grooves 2, a sealing plate 15 is arranged at the opening of each transparent groove 14, when the culture test tubes 5 in the incubator 1 are subjected to light transmission treatment, the sealing plate 15 at the opening of each transparent groove 14 can be opened, and as the inner walls of the transparent grooves 14 are transparent, the transparent grooves 14 are positioned between two adjacent culture grooves 2, the culture test tubes 5 can be observed through the transparent grooves 14 and transmitted, and the sealing plate 15 can be clamped into the opening of the transparent grooves 14 to avoid light.
As a further scheme of the invention, a collecting bin 16 is arranged below the inside of the incubator 1, an electromagnetic valve 17 is arranged at the top of the inner wall of the collecting bin 16, the electromagnetic valve 17 penetrates through the bottom of the inner wall of the incubator 2, and a discharge main valve 18 is arranged at the bottom of the inner wall of the collecting bin 16. When the culture tube 5 is broken or overflows liquid, the liquid flows into the electromagnetic valve 17 below along the culture tank 2, the electromagnetic valve 17 is opened to lead the liquid into the collecting bin 16, the collecting bin 16 can collect the liquid in all the culture tanks 2 in a concentrated manner, the culture tank 1 can be discharged by opening the discharge main valve 18 at the bottom, each electromagnetic valve 17 is independently designed, and the cross contamination caused by the conduction of a plurality of culture tanks 2 is prevented, so that the culture work of cells is influenced.
As a further scheme of the invention, the erection assembly 3 comprises a stand column 301, a transverse plate 303 and a supporting plate 305 are fixedly connected between the stand column 301 and a sealing cover 4, two limiting separation plates 304 are arranged between the supporting plate 305 and the transverse plate 303, the limiting separation plates 304 are arranged on opposite surfaces of the stand column 301 and the sealing cover 4, a plurality of clamping holes 302 are formed in the transverse plate 303, a plurality of ball grooves 306 and leak holes 307 are formed in the supporting plate 305, the bottom ends of the culture test tubes 5 penetrate through the clamping holes 302 and the limiting separation plates 304 and are positioned in the ball grooves 306, the stand column 301, the transverse plate 303 and the supporting plate 305 are slidably connected in the culture tank 2, and the top ends of the culture test tubes 5 are higher than the clamping holes 302, so that the culture test tubes 5 can be conveniently taken and placed.
The culture tube 5 with cells is placed in the erection assembly 3, the bottom end of the culture tube 5 penetrates through the clamping hole 302 and the limiting partition plate 304 and is positioned in the ball groove 306, the culture tube 5 is limited through the ball groove 306 and the clamping hole 302 and is supported by the limiting partition plate 304, the erection stability of the culture tube 5 is guaranteed, and when the culture tube 5 is damaged or overflows, liquid flows out along the leak hole 307.
As a further scheme of the invention, the locking assembly 7 comprises a sliding plate 701, the top and the bottom of the sealing cover 4 are respectively provided with a sliding groove 6, a plurality of clamping grooves 9 are respectively arranged above and below the outer wall of the incubator 1, the sliding plate 701 is slidably connected in the sliding groove 6, a clamping block 702 is fixedly connected on the sliding plate 701, the clamping block 702 is clamped in the clamping groove 9, one side of the clamping block 702 is in a cambered surface design and the other side of the clamping block 702 is in a plane design, a pull rod 703 is fixed in the sliding plate 701, the end part of the pull rod 703 is fixed with the handle 8, a supporting spring 704 is sleeved outside the pull rod 703, and the supporting spring 704 is fixed between the sealing cover 4 and the inner wall of the sliding plate 701. The sliding plate 701 is supported by the elasticity of the supporting spring 704, so that the sliding plate 701 drives the clamping block 702 to be clamped into the clamping groove 9, the purpose of locking the sealing cover 4 is achieved, the operation convenience is improved, the opening of the culture groove 2 can be sealed by the sealing cover 4, and external dust and impurities are prevented from entering the culture groove 2.
As a further scheme of the invention, the automatic culture analyzer 10 comprises an automatic control module 101, a fixed column 102 is arranged in the middle of the automatic control module 101, the fixed column 102 is arranged in a central groove 11, a clamping sleeve 103 is arranged outside the automatic control module 101, the clamping sleeve 103 is connected to the top of the incubator 1, a group of online observers 104 are respectively arranged at the bottom of the automatic control module 101 corresponding to the positions of a plurality of culture grooves 2, the number of each group of online observers 104 is five, and each online observer 104 is positioned right above a culture tube 5. Since each on-line observer 104 is located right above the culture tube 5, thereby ensuring the observation precision, the on-line observer 104 can observe the cells in the culture tube 5 on line and transmit signals to the automatic control module 101, so that the culture state can be observed and analyzed in real time;
the clamping sleeve 103 is held and pulled upwards, so that the clamping sleeve 103 drives the fixing column 102 to slide in the central groove 11 through the automatic control module 101, and the automatic control module 101 drives the plurality of on-line observers 104 to move out of the incubator 1, so that the automatic culture analyzer 10 is conveniently detached, and cleaning and maintenance work are facilitated.
As a further scheme of the invention, the supporting component 13 comprises a fixed ring 131, four supporting rods 132 are fixed on the fixed ring 131, universal wheels 133 are installed at the bottom ends of the supporting rods 132, four grooves 12 are formed in the bottom of the incubator 1, the top ends of the supporting rods 132 are slidably connected in the grooves 12, buffer springs 134 are sleeved outside the supporting rods 132, the buffer springs 134 are fixed between the bottom of the incubator 1 and the fixed ring 131, damping springs 135 are fixed at the top ends of the supporting rods 132, and the damping springs 135 are installed in the grooves 12. The supporting rod 132 extrudes the buffer spring 134 through the fixed ring 131, and the buffer spring 134 can provide a reverse acting force for the fixed ring 131 after being pressed, can play the effect of damping the incubator 1, and the damping spring 135 in the matching groove 12 supports the end part of the supporting rod 132, so that the stability of the culture tube 5 is prevented from being influenced by the overlarge amplitude of the incubator 1.
The application method of the full-automatic cell culture device comprises the following steps:
When full-automatic cell culture is carried out, a culture tube 5 filled with cells is placed in an erection assembly 3, the bottom end of the culture tube 5 penetrates through a clamping hole 302 and a limiting baffle 304 and is positioned in a ball groove 306, the culture tube 5 is limited through the ball groove 306 and the clamping hole 302 and is supported by the limiting baffle 304, the erection stability of the culture tube 5 is ensured, the top end of the culture tube 5 is higher than the clamping hole 302, after the placement work of the culture tube 5 is completed, a sealing cover 4 is pushed, the sealing cover 4 drives the culture tube 5 to move through a transverse plate 303 and a supporting plate 305, and the culture tube 5 can enter a culture tank 2 in a culture box 1;
When the sealing cover 4 moves, the locking assembly 7 is driven to approach the incubator 1, when the clamping block 702 in the locking assembly 7 contacts with the side wall of the incubator 1, as one side of the clamping block 702, which is close to the incubator 1, is designed to be an arc surface, the clamping block 702 can drive the sliding plate 701 to slide into the sliding groove 6, when the sealing cover 4 is tightly attached to the side wall of the incubator 1, the sliding plate 701 is supported by the elastic force of the supporting spring 704, the sliding plate 701 drives the clamping block 702 to be clamped into the clamping groove 9, the purpose of locking the sealing cover 4 is achieved, the sealing cover 4 can seal the opening of the incubator 2, external dust impurities are prevented from entering the incubator 2, the erection assembly 3 is installed in each incubator 2, the erection assemblies 3 at other positions of the incubator 1 are operated in the same way, further, the culture tubes 5 are conveniently fed into the incubator 1, each incubator 2 is independently designed, the cells in different time periods and different types of cells can be independently cultured and taken and placed, and all the independently designed assemblies 3 are integrated into a single incubator 1 to fully-automatically culture the cells in the culture tubes 5;
When the cells in the culture test tube 5 are cultured through the incubator 1, the bottom end of the online observer 104 in the automatic culture analyzer 10 is communicated with the culture tank 2, each online observer 104 is positioned right above the culture test tube 5, the cells in the culture test tube 5 are observed online through the online observer 104 and signals are transmitted to the automatic control module 101, the automatic culture analyzer 10 can analyze real-time data, when the culture test tube 5 in the incubator 1 needs to be subjected to light transmission treatment, the sealing plate 15 at the opening of the light transmission groove 14 can be opened, and the inner wall of the light transmission groove 14 is transparent, the light transmission groove 14 is positioned between two adjacent culture tanks 2, the culture test tube 5 can be observed through the light transmission groove 14 and transmitted, and the sealing plate 15 can be clamped into the opening of the light transmission groove 14 to avoid light;
When the culture tube 5 is broken or overflows, the liquid flows into the electromagnetic valve 17 below along the culture tank 2 through the leak hole 307, the electromagnetic valve 17 is opened to lead the liquid into the collecting bin 16, so that the collecting bin 16 can collect the liquid in all the culture tanks 2 in a concentrated manner, the culture tank 1 can be discharged through the discharge main valve 18 at the bottom of the collecting bin, each electromagnetic valve 17 is independently designed, and the cross contamination caused by the conduction of a plurality of culture tanks 2 is prevented, thereby influencing the culture work of cells;
When the culture device is transferred, the incubator 1 is pushed to enable the universal wheels 133 in the support assembly 13 to roll on the ground, in the moving process of the incubator 1, the universal wheels 133 can squeeze the support rods 132, the support rods 132 squeeze the buffer springs 134 through the fixed rings 131, the buffer springs 134 can provide a reverse acting force for the fixed rings 131 after being pressed, the shock absorption effect on the incubator 1 can be achieved, the end parts of the support rods 132 are supported by the damping springs 135 in the grooves 12, and the influence on the stability of the culture tubes 5 due to overlarge amplitude of the incubator 1 is prevented;
When taking out the culture tube 5, through squeezing two handles 8, make two handles 8 pull slide 701 through pull rod 703 respectively, make slide 701 drive fixture block 702 break away from draw-in groove 9, release the locking state between sealed lid 4 and the incubator 1 promptly, outwards pull handle 8 can take out culture tube 5 and cultivate groove 2, moreover whole erect subassembly 3 and culture tube 5 can shift out culture groove 2 completely, and then the convenience is to the work of putting of getting out of culture tube 5, when tearing down automatic culture analyzer 10, grip cutting ferrule 103 and upwards pulling, make cutting ferrule 103 drive fixed column 102 through automatic control module 101 and slide in central groove 11, automatic control module 101 then drives a plurality of online observers 104 and shifts out incubator 1, can tear down automatic culture analyzer 10, so that the daily cleanness and the maintenance work of device.
Claims (8)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202510065564.1A CN119842483A (en) | 2025-01-15 | 2025-01-15 | Full-automatic cell culture device and application method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202510065564.1A CN119842483A (en) | 2025-01-15 | 2025-01-15 | Full-automatic cell culture device and application method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN119842483A true CN119842483A (en) | 2025-04-18 |
Family
ID=95360960
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202510065564.1A Pending CN119842483A (en) | 2025-01-15 | 2025-01-15 | Full-automatic cell culture device and application method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN119842483A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN120505201A (en) * | 2025-05-30 | 2025-08-19 | 江苏长禾胶囊有限公司 | Biochemical incubator with multiple bins and method thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN207002731U (en) * | 2017-06-22 | 2018-02-13 | 侯翠萍 | A kind of microbiological incubator for medical research |
| CN212688086U (en) * | 2020-05-11 | 2021-03-12 | 天津市津华生物科技有限公司 | Culture apparatus for observing growth process of biological stem cells in each period in classified manner |
| CN214496353U (en) * | 2021-02-26 | 2021-10-26 | 深圳市金灿华实业有限公司 | Cell culture device with cell culture pipe support |
| CN214830274U (en) * | 2021-07-14 | 2021-11-23 | 玉林师范学院 | A photosynthetic microorganism culture device |
| CN215103302U (en) * | 2021-06-03 | 2021-12-10 | 滁州学院 | Microbial cultivation device |
| CN219490036U (en) * | 2023-04-04 | 2023-08-08 | 山东致圣生物科技有限公司 | Cell incubator convenient to get and put cell culture dish |
| CN221275749U (en) * | 2023-10-31 | 2024-07-05 | 山东格林医学科技有限公司 | Cultivation device for pathogenic microorganisms |
-
2025
- 2025-01-15 CN CN202510065564.1A patent/CN119842483A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN207002731U (en) * | 2017-06-22 | 2018-02-13 | 侯翠萍 | A kind of microbiological incubator for medical research |
| CN212688086U (en) * | 2020-05-11 | 2021-03-12 | 天津市津华生物科技有限公司 | Culture apparatus for observing growth process of biological stem cells in each period in classified manner |
| CN214496353U (en) * | 2021-02-26 | 2021-10-26 | 深圳市金灿华实业有限公司 | Cell culture device with cell culture pipe support |
| CN215103302U (en) * | 2021-06-03 | 2021-12-10 | 滁州学院 | Microbial cultivation device |
| CN214830274U (en) * | 2021-07-14 | 2021-11-23 | 玉林师范学院 | A photosynthetic microorganism culture device |
| CN219490036U (en) * | 2023-04-04 | 2023-08-08 | 山东致圣生物科技有限公司 | Cell incubator convenient to get and put cell culture dish |
| CN221275749U (en) * | 2023-10-31 | 2024-07-05 | 山东格林医学科技有限公司 | Cultivation device for pathogenic microorganisms |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN120505201A (en) * | 2025-05-30 | 2025-08-19 | 江苏长禾胶囊有限公司 | Biochemical incubator with multiple bins and method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN202177536U (en) | Sheet production auxiliary equipment provided with natural settling device and used in liquid-based cytology sheet production system | |
| CN216375588U (en) | Kit convenient to arrange for clinical laboratory | |
| CN205958293U (en) | Dirty water environmental protection sampling test analysis display system | |
| CN204536085U (en) | A kind of year net stationary installation for the dyeing of Electronic Speculum ultra-thin section batch | |
| CN211870333U (en) | Waste liquid collecting tank for biological research | |
| CN221056118U (en) | Raw material assaying and sampling device | |
| CN209271489U (en) | A kind of droplet collection device of chip | |
| CN206920171U (en) | quantitative sampling dilution device | |
| CN209807673U (en) | Art teaching is with show board | |
| CN206321496U (en) | A kind of Grain stain box easy to use | |
| CN212321187U (en) | Cistanche salsa processing extract sampling device | |
| CN207085924U (en) | A kind of chemistry teaching laboratory apparatus | |
| CN218435739U (en) | Quick sampler of probiotics | |
| CN223738049U (en) | Culture dish convenient to get and put cell climbing piece | |
| CN222034785U (en) | Mixing bottle device suitable for blood cell analysis | |
| CN223783964U (en) | A biochemical testing sampling device | |
| CN112662729A (en) | Total coliform group determination kit and use method thereof | |
| CN216808813U (en) | A student uses many functional safety culture dish for biological assay | |
| CN217930913U (en) | A fast adding fixative box for surface water collection, measurement and separation | |
| CN223679175U (en) | Quadruple detection kit | |
| CN218067317U (en) | Slide glass sampling and dyeing device for inspection | |
| CN220724199U (en) | Cell culture plate | |
| CN219930053U (en) | Microorganism culture and detection device | |
| CN212988984U (en) | Waste liquid water conservancy diversion slide frame that bacterium gram staining used | |
| CN223526047U (en) | Detachable type stool collector |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |