High-efficient concentrated purification device of biological enzyme
Technical Field
The application relates to the technical field of biological enzyme purification, in particular to a high-efficiency concentration and purification device for biological enzyme.
Background
Biological enzymes, commonly referred to as enzymes for short, are proteins with biological catalytic effects, and currently, in industries such as biological pharmacy and food processing, the biological enzymes are used as important catalytic tools, and high-efficiency purification is a key link for improving product quality and reducing production cost, however, the existing biological enzyme purification technology often suffers from problems of low purity, low recovery rate and the like, so that large-scale industrial application of the biological enzymes is limited, and a biological enzyme concentration and purification device is a device for improving the purity and activity of the biological enzymes, and relates to multiple steps such as filtration, separation, concentration, refining and the like.
The biological enzyme purification technology commonly used at present is to filter the biological enzyme solution, and some filtering devices adopt a multi-layer filtering technology, and a coarse particle filtering layer, a grease adsorption layer, a fine particle filtering layer and a filtering screen layer are sequentially distributed from top to bottom in a filtering tank, so that conventional impurities can be filtered from the biological enzyme preparation to the greatest extent through the design.
In the conventional biological enzyme solution filtering process, the solution is generally directly introduced into a filtering tank, the solution directly falls on a filter screen in the filtering tank, the solution impacts the filter screen, then the solution passes through the filter screen, impurities in the solution are left on the filter screen, and then the filtered solution directly flows out of the filtering tank, but when the solution is introduced into the filtering tank, the filter screen is generally directly fixed in the filtering tank, the solution directly falls on a part of the area of the filter screen, other areas of the filter screen are difficult to contact with the solution, the utilization rate of the filter screen is low, and the part of the filter screen which is often contacted with the solution is possibly blocked by the impurities.
Disclosure of Invention
The application aims to solve the problems that when the solution is introduced into a filter tank, a filter screen is generally directly fixed in the filter tank, and when the solution is directly dropped on a part of the filter screen, other areas of the filter screen are difficult to contact with the solution, the utilization rate of the filter screen is low, and the part of the filter screen which is frequently contacted with the solution is possibly blocked by impurities.
The application adopts the following technical scheme for realizing the purposes:
the utility model provides a high-efficient concentrated purification device of biological enzyme, includes the purification jar, purification jar bottom is fixed with the supporting leg, purification jar top is fixed with the pan feeding pipe, purification jar bottom is fixed with the discharge pipe, purification jar side is fixed with the observation window, be provided with movable filter mechanism in the purification jar, be provided with auxiliary filter mechanism in the purification jar.
Through adopting above-mentioned technical scheme, send into the purification jar with pretreatment solution in utilizing the pan feeding pipe, with purification jar filling, then utilize to remove filter mechanism from the bottom of purification jar to begin to upwards remove, at the in-process that removes filter mechanism and upwards move, solution evenly passes from removing filter mechanism, remove filter mechanism simultaneously and upwards move the in-process that drives liquid and stir, thereby can let solution evenly pass and remove filter mechanism, improve solution filterable efficiency, impurity that can reduce to filter simultaneously causes the jam, influence solution normal filterable possibility.
Further, remove filtering mechanism includes a plurality of filter screen one of setting in the purification jar, be fixed with the holding ring on the filter screen one, a plurality of be provided with the removal subassembly between holding ring and the purification jar, a plurality of be provided with on the holding ring supplementary stirring mechanism.
Through adopting above-mentioned technical scheme, utilize the removal subassembly to drive a plurality of supporting ring, let the supporting ring move first filter screen, let first filter screen remove in solution, impurity in the first pair of solution of filter screen filters, at the in-process that the supporting ring moves first filter screen of supporting ring, the supporting ring moves supplementary stirring subassembly, supplementary stirring subassembly carries out the vortex stirring to the solution of first top of filter screen, let the impurity that first filter screen filtered remove from a filter screen surface, thereby can let the abundant and first contact of filter screen of solution that needs to filter, reduce traditional purification and fix first filter screen, let solution pass first filter screen, result in the first part of the impact filter screen of solution only singleness, result in first filter screen to receive impurity jam, the possibility of filtration inefficiency, simultaneously can reduce the possibility of impurity jam first filter screen.
Further, remove the subassembly including setting up the removal ring in the purification jar, a plurality of go-between with remove ring inner wall fixed connection, be fixed with the support frame on the purification jar, be fixed with on the support frame and remove driving motor, remove driving motor's output and run through the purification jar, and be fixed with and remove the threaded rod, remove the threaded rod and run through the removal ring, and with remove ring threaded connection.
Through adopting above-mentioned technical scheme, remove the threaded rod and drive the removal ring, remove the ring and remove in the purification jar, remove the ring and drive a plurality of support ring, let the support ring to move the first removal of filter screen to can let the solution in the purification jar fully with the first abundant contact of filter screen, make things convenient for the first removal in the solution of filter screen, filter the impurity in the solution.
Further, the auxiliary stirring mechanism comprises two supporting rods symmetrically fixed on the supporting ring, two symmetrical spoilers are arranged between the two supporting rods, and a rotating piece is arranged between the two supporting rods and the two spoilers.
Through adopting above-mentioned technical scheme, the bracing piece drives rotation piece and spoiler, rotates the in-process that the piece removed at rotation, rotates the piece and drives the spoiler and rotate in the top of filter screen one, lets the spoiler stir the solution to can let the spoiler take up the impurity activity on the filter screen one, reduce the impurity and block up the possibility of filter screen one.
Further, the rotating piece comprises a rotating ring fixed between the two supporting rods, a rotating sleeve is connected to the rotating ring in a rotating mode, the two spoilers are fixedly connected with the rotating sleeve, a rotating threaded rod is fixed in the purifying tank and penetrates through the rotating sleeve and is in threaded connection with the rotating sleeve.
Through adopting above-mentioned technical scheme, the supporting ring drives the bracing piece, and the bracing piece drives the swivel becket, and the swivel becket drives the rotation sleeve pipe, and the rotation sleeve pipe removes along rotating the threaded rod, under the effect of rotating the threaded rod, lets the rotation sleeve pipe rotate on the swivel becket to can conveniently let the rotation sleeve pipe drive the spoiler and rotate.
Further, a plurality of uniformly distributed turbulence holes are formed in the turbulence plate, and the turbulence holes face the rotation direction of the turbulence plate.
Through adopting above-mentioned technical scheme, the vortex hole on the spoiler carries out further vortex to the solution to can let the solution be further disturbed when the spoiler rotates, further reduce the possibility that impurity attached to on filter screen one.
Further, the auxiliary filtering mechanism comprises a second filter screen arranged in the purifying tank, a reciprocating ring is fixed on the second filter screen, and a reciprocating assembly is arranged between the reciprocating ring and the purifying tank.
Through adopting above-mentioned technical scheme, let filter screen two filter solution again at the exhaust in-process, filter screen two is the microfiltration membrane, lets simultaneously and removes the threaded rod and drive the removal ring, removes the threaded rod and drives reciprocating subassembly, and reciprocating subassembly drives reciprocating ring and reciprocates, and reciprocating ring moves filter screen two round trip movement to can reduce the impurity in the solution further, can reduce the possibility that impurity blockked up filter screen two simultaneously.
Further, the reciprocating assembly comprises a reciprocating lantern ring fixed in the reciprocating tank, the reciprocating ring is in sliding connection with the reciprocating lantern ring, a reciprocating threaded rod is rotatably connected to the reciprocating lantern ring, and one end of the reciprocating threaded rod penetrates through the reciprocating lantern ring and is fixedly connected with the movable threaded rod.
Through adopting above-mentioned technical scheme, remove the threaded rod and drive reciprocal threaded rod, reciprocal threaded rod drives the shuttle ring, lets the shuttle ring make a round trip under the restriction of reciprocal lantern ring remove to can conveniently let reciprocal girdle move filter screen two reciprocating movements.
In summary, the present application includes at least one of the following advantages;
1. According to the application, the purification tank is filled, the movable driving motor drives the movable threaded rod to rotate, the movable threaded rod drives the movable ring, the movable ring moves in the purification tank, the movable ring drives the plurality of support rings, the support rings drive the first filter screen to move, the first filter screen moves from the bottom of the first purification tank to the upper part of the purification tank, the first filter screen passes through the solution, the first filter screen filters impurities in the solution, meanwhile, in the process of moving the support rings, the support rings drive the supporting rods, the rotating piece drives the spoiler to rotate, the spoiler agitates the impurities on the first filter screen, the possibility that the first filter screen is blocked by the impurities is reduced, the filtered solution is discharged from the discharge pipe after the first filter screen moves to the uppermost part of the purification tank, the solution to be filtered is fully contacted with the first filter screen, the traditional purification is reduced, the solution passes through the first filter screen, the solution is only one part of the first filter screen is impacted, the first filter screen is blocked by the impurities, the possibility of low filtering efficiency is reduced, and the possible purpose that the first filter screen is blocked by the impurities.
2. According to the application, the second filter screen filters the solution again in the process of discharging the solution, the second filter screen is a micro-filtration membrane, the movable threaded rod drives the movable ring, the movable ring drives the first filter screen to reset, the movable threaded rod drives the reciprocating threaded rod in the resetting process, and the reciprocating threaded rod drives the reciprocating ring to move back and forth under the limit of the reciprocating lantern ring, so that the purposes of further reducing impurities in the solution and reducing the possibility of blocking the second filter screen by the impurities are achieved.
3. According to the application, the supporting ring drives the supporting rod in the moving process of the supporting ring, the supporting rod drives the rotating ring, the rotating ring drives the rotating sleeve, the rotating sleeve moves along the rotating threaded rod, the rotating sleeve rotates on the rotating ring under the action of the rotating threaded rod, and the rotating sleeve drives the spoiler to rotate, so that the purpose that the rotating sleeve can conveniently drive the spoiler to rotate is achieved.
Drawings
FIG. 1 is a schematic diagram of a first perspective structure of a device for efficiently concentrating and purifying biological enzymes in the application;
FIG. 2 is a schematic diagram of a first internal structure of the efficient concentration and purification device for biological enzymes in the application;
FIG. 3 is a schematic diagram of a first internal structure of the efficient concentration and purification device for biological enzymes in the application;
FIG. 4 is an enlarged schematic view of the application at A in FIG. 2;
fig. 5 is an enlarged schematic view of the application at B in fig. 3.
Reference numerals illustrate:
1. The purifying tank comprises a purifying tank body, a supporting leg, a feeding pipe, a discharging pipe, a moving filtering mechanism, a supporting ring, a filter screen I, a moving component, a moving ring, a filter screen II, a filter screen 53, a moving component, a moving ring, a 532, a supporting frame, a 533, a moving threaded rod, a 534, a moving driving motor, a 54, an auxiliary stirring mechanism, a 541, a supporting rod, a 542, a spoiler, a 543, a rotating piece, a 5431, a rotating ring, a 5432, a rotating sleeve, a 5433, a rotating threaded rod, a6, an auxiliary filtering mechanism, a 61, a filter screen II, a 62, a reciprocating ring, a 63, a reciprocating component, a 631, a reciprocating collar, a 632, a reciprocating threaded rod, a 7 and an observation window.
Detailed Description
The application is described in further detail below with reference to fig. 1-5.
The embodiment of the application discloses a high-efficiency concentration and purification device for biological enzymes.
Referring to fig. 1,2 and 3, a high-efficient concentrated purification device of biological enzyme, including purification jar 1, purification jar 1 bottom is fixed with supporting leg 2, and purification jar 1 top is fixed with pan feeding pipe 3, and purification jar 1 bottom is fixed with discharge pipe 4, and purification jar 1 side is fixed with observation window 7, is provided with removal filter mechanism 5 in the purification jar 1, is provided with auxiliary filter mechanism 6 in the purification jar 1.
When using this concentrated purification device to carry out purification to biological enzyme, firstly send into purification jar 1 with the preliminary treatment solution with the pan feeding pipe, with purification jar 1 filling, then utilize the removal filter mechanism 5 to follow purification jar 1's bottommost and begin to upwards remove, in the in-process that removes filter mechanism 5 to reciprocate from removing filter mechanism 5, remove filter mechanism 5 and filter the impurity in the solution, simultaneously remove filter mechanism 5 and drive liquid in the in-process that reciprocates and stir, reduce the possibility that the impurity that removes filter mechanism 5 and filter influences the filtration, after the solution filters, discharge the biological enzyme solution after the completion to the outside from discharge pipe 4 and store reserve, when solution flows out from purification jar 1's discharge pipe 4, the solution passes auxiliary filter mechanism 6, auxiliary filter mechanism 6 carries out further filtration to the solution, simultaneously when removing filter mechanism 5 and reset, let remove filter mechanism 5 drive auxiliary filter mechanism 6 reciprocating motion, reduce the possibility that auxiliary filter mechanism 6 appears blocking, through with purification jar 1, then remove filter mechanism 5 and drive liquid in the in-process of removing filter mechanism 5, can cause filtering mechanism 5 to filter the impurity in the removal mechanism 5, can filter mechanism 5 to filter the normal filtration in the filter mechanism through removing filter mechanism that removes filter mechanism 1, can filter the impurity in the filter mechanism 5, the normal filter mechanism is removed in the filter mechanism.
Referring to fig. 2, 3 and 4, the mobile filtering mechanism 5 includes a plurality of first filter screens 52 disposed in the purification tank 1, a support ring 51 is fixed on the first filter screens 52, a mobile component 53 is disposed between the plurality of support rings 51 and the purification tank 1, and an auxiliary stirring mechanism 54 is disposed on the plurality of support rings 51.
The first filter screen 52 fixed on the support ring 51 is a micro-filtration membrane, when the pretreated solution is poured into the purification tank 1, the first filter screens 52 are positioned at the bottom of the purification tank 1 under the support of the support ring 51, after the purification tank 1 is filled with the solution, the plurality of support rings 51 are driven by the moving component 53, the first filter screen 52 is driven by the support ring 51 to move in the solution, the first filter screen 52 filters impurities in the solution, the support ring 51 drives the auxiliary stirring component to stir the solution above the first filter screen 52, the auxiliary stirring component carries out turbulent stirring on the solution above the first filter screen 52, the filtered impurities of the first filter screen 52 move away from the surface of the first filter screen 52, after the purification tank 1 is filled with the solution, the first filter screen 52 is fully contacted with the solution, the first filter screen 52 is driven by the moving component 53, the first support ring 51 is simultaneously moved in the solution, the first filter screen 52 is driven by the auxiliary stirring component, the solution above the first filter screen 52 is further stirred by the traditional stirring component, impurities which need to be filtered are fully contacted with the first filter screen 52, the impurity can be reduced, the first filter screen 52 can be blocked by the impurity can be effectively removed, and the first filter screen 52 can be blocked by the fixed filter screen 52, and the impurity can be reduced.
Referring to fig. 2 and 3, the moving assembly 53 includes a moving ring 531 disposed in the purifying tank 1, a plurality of supporting rings 51 fixedly connected with an inner wall of the moving ring 531, a supporting frame 532 fixed on the purifying tank 1, a moving driving motor 534 fixed on the supporting frame 532, an output end of the moving driving motor 534 penetrating through the purifying tank 1 and a moving threaded rod 533 fixed on the purifying tank, the moving threaded rod 533 penetrating through the moving ring 531 and being in threaded connection with the moving ring 531.
After filling the purification tank 1 with the solution, let the removal driving motor 534 drive the removal threaded rod 533 rotate, remove threaded rod 533 drive and remove the ring 531, remove the ring 531 and remove in purification tank 1, remove the ring 531 and drive a plurality of support ring 51, let the support ring 51 drive filter screen one 52 remove, after filling the purification tank 1, let remove the threaded rod 533 drive remove the ring 531, let remove the ring 531 drive filter screen one 52 remove, thereby can let the solution in the purification tank 1 fully contact with filter screen one 52, make things convenient for filter screen one 52 to remove in the solution, filter the impurity in the solution.
Referring to fig. 2, 3 and 4, the auxiliary agitating mechanism 54 includes two supporting bars 541 symmetrically fixed to the supporting ring 51, two symmetrical spoilers 542 are provided between the two supporting bars 541, and a rotating member 543 is provided between the two supporting bars 541 and the two spoilers 542.
In the process that the moving ring 531 drives the supporting ring 51 to move, the supporting ring 51 drives the supporting rod 541, the supporting rod 541 drives the rotating piece 543 and the spoiler 542, in the process that the rotating piece 543 moves, the rotating piece 543 drives the spoiler 542 to rotate above the first filter screen 52, the spoiler 542 agitates the solution, the agitated solution drives impurities on the first filter screen 52 to move, the spoiler 542 rotates on the first filter screen 52 when the supporting ring 51 moves, and accordingly the spoiler 542 can bring up the impurities on the first filter screen 52 to move, and the possibility that the impurities block the first filter screen 52 is reduced.
Referring to fig. 2,3 and 4, the rotating member 543 includes a rotating ring 5431 fixed between two support rods 541, a rotating sleeve 5432 is rotatably connected to the rotating ring 5431, two spoilers 542 are fixedly connected to the rotating sleeve 5432, a rotating threaded rod 5433 is fixed in the purification tank 1, and the rotating threaded rod 5433 penetrates the rotating sleeve 5432 and is in threaded connection with the rotating sleeve 5432.
In the process of moving the support ring 51, the support ring 51 drives the support rod 541, the support rod 541 drives the rotating ring 5431, the rotating ring 5431 drives the rotating sleeve 5432, the rotating sleeve 5432 moves along the rotating threaded rod 5433, under the action of the rotating threaded rod 5433, the rotating sleeve 5432 rotates on the rotating ring 5431, the rotating sleeve 5432 drives the spoiler 542 to rotate, the support rod 541 drives the rotating ring 5431, the rotating ring 5431 drives the rotating sleeve 5432, and the rotating sleeve 5432 rotates when moving along the rotating threaded rod 5433, so that the rotating sleeve 5432 can conveniently drive the spoiler 542 to rotate.
Referring to fig. 2 and 4, a plurality of uniformly distributed turbulence holes are formed on the turbulence plate 542, and the turbulence holes face the rotation direction of the turbulence plate 542. When the rotating sleeve 5432 drives the spoiler 542 to rotate, the spoiler holes on the spoiler 542 further spoiler the solution, so that the impurities on the first filter screen 52 move along with the flow of the solution, and the spoiler 542 is provided with a plurality of spoiler holes, so that the solution can be further disturbed when the spoiler 542 rotates, and the possibility that the impurities are attached to the first filter screen 52 is further reduced.
Referring to fig. 2,3 and 5, the auxiliary filtering mechanism 6 includes a second filter screen 61 provided in the purification tank 1, a reciprocating ring 62 is fixed to the second filter screen 61, and a reciprocating assembly 63 is provided between the reciprocating ring 62 and the purification tank 1.
After the first filter screen 52 moves to the uppermost part of the purification tank 1, the filtered solution is discharged from the discharge pipe 4, the second filter screen 61 filters the solution again in the discharging process, the second filter screen 61 is a micro-filtration membrane, meanwhile, the movable threaded rod 533 drives the movable ring 531, the movable ring 531 drives the first filter screen 52 to reset, in the resetting process, the movable threaded rod 533 drives the reciprocating assembly 63, the reciprocating assembly 63 drives the reciprocating ring 62 to move back and forth, the reciprocating ring 62 drives the second filter screen 61 to move back and forth, impurities on the second filter screen 61 are rocked, the second filter screen 61 filters the solution again, and when the first filter screen 52 resets, the movable threaded rod 533 drives the reciprocating assembly 63, and the reciprocating assembly 63 drives the second filter screen 61 to move back and forth, so that impurities in the solution can be further reduced, and the possibility that the second filter screen 61 is blocked by the impurities can be reduced.
Referring to fig. 3 and 5, the reciprocating assembly 63 includes a reciprocating collar 631 fixed in the reciprocating tank, the reciprocating ring 62 is slidably connected with the reciprocating collar 631, a reciprocating threaded rod 632 is rotatably connected to the reciprocating collar 631, and one end of the reciprocating threaded rod 632 penetrates the reciprocating collar 631 and is fixedly connected with the moving threaded rod 533.
When the movable threaded rod 533 rotates, the movable threaded rod 533 drives the reciprocating threaded rod 632, the reciprocating threaded rod 632 drives the reciprocating ring 62, the reciprocating ring 62 moves back and forth under the limitation of the reciprocating collar 631, and the reciprocating ring 62 moves under the limitation of the reciprocating collar 631 by the reciprocating threaded rod 632 driving the reciprocating ring 62, so that the reciprocating ring 62 can conveniently drive the filter screen two 61 to reciprocate.
Working principle: when the concentration and purification device is used for purifying biological enzymes, firstly, a pretreatment solution is sent into the purification tank 1 by using a material inlet pipe, the purification tank 1 is filled, a movable driving motor 534 drives a movable threaded rod 533 to rotate, the movable threaded rod 533 drives a movable ring 531, the movable ring 531 moves in the purification tank 1, the movable ring 531 drives a plurality of supporting rings 51, the supporting rings 51 drive a first filter screen 52 to move, the first filter screen 52 moves from the bottom of the first purification tank 1 to the upper part of the purification tank 1, the first filter screen 52 passes through the solution, the first filter screen 52 filters impurities in the solution, meanwhile, the supporting rings 51 drive a supporting rod 541, the supporting rod 541 drives a rotating ring 5431, the rotating ring 5431 drives a rotating sleeve 5432, the rotating threaded rod 5433 moves along the rotating threaded rod 5433, the rotating sleeve 5432 drives a rotating threaded rod 5433, the rotating spoiler 542 to stir the impurities on the first filter screen 52, the impurities on the first filter screen 52 can be reduced, the first filter screen 52 can move to the uppermost filter screen 533, the first filter screen is reset to the second filter screen 533, the second filter screen is driven to reciprocate back and forth in the process of the second filter screen is driven by the rotating threaded rod 531, and the reciprocating to move back and forth in the process of the second filter rod 632 is driven by the rotating threaded rod 531, and the reciprocating the threaded rod is reciprocally moved by the threaded rod 531 is reciprocally moved by the reciprocating the threaded rod 61, and the filter rod has the reciprocating filter rod 531 is reciprocally moved back and moved by the filter rod 61 is reciprocally back and moved by the filter rod 61.