CN119286649B - A cell culture dish - Google Patents
A cell culture dish Download PDFInfo
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- CN119286649B CN119286649B CN202411837902.0A CN202411837902A CN119286649B CN 119286649 B CN119286649 B CN 119286649B CN 202411837902 A CN202411837902 A CN 202411837902A CN 119286649 B CN119286649 B CN 119286649B
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- culture dish
- scraping
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- detachably
- driving
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/08—Cleaning containers, e.g. tanks
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/08—Cleaning containers, e.g. tanks
- B08B9/087—Cleaning containers, e.g. tanks by methods involving the use of tools, e.g. brushes, scrapers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/08—Cleaning containers, e.g. tanks
- B08B9/093—Cleaning containers, e.g. tanks by the force of jets or sprays
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M39/00—Means for cleaning the apparatus or avoiding unwanted deposits of microorganisms
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- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Mechanical Engineering (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
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- General Engineering & Computer Science (AREA)
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- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a cell culture dish, which belongs to the field of culture dishes and comprises a culture dish, a driving component and a connecting frame, wherein a bearing cavity is formed in the culture dish, and the driving component comprises the connecting frame which is detachably arranged on the culture dish. According to the cell culture dish, the stirring and mixing component can be triggered simultaneously to stir and mix liquid and the linkage scraping component can move downwards and upwards to scrape, the stirring and mixing component is arranged to drive the driving wheel to rotate after the driving motor drives the driving rod to rotate so that the driving wheel drives the driven wheel to rotate, the driven wheel drives the dragon blade to rotate after the driven wheel rotates so as to mix and stir the aqueous solution and the cleaning liquid in the bearing cavity of the culture dish, and the mixed solution of the aqueous solution and the cleaning liquid can be driven to shake in the mixing and stirring process so as to enhance the flushing effect on the inner bottom wall and the inner side wall of the culture dish.
Description
Technical Field
The invention belongs to the field of culture dishes, and particularly relates to a cell culture dish.
Background
Cells are the fundamental structural and functional units of an organism. The cell body shape is very tiny, and can be seen under a microscope, and the shape is various. Mainly comprises a cell nucleus and cytoplasm, and has a cell membrane on the surface. The cell wall is arranged outside the cell membrane of the higher plant, the plastid is arranged in the cytoplasm, and chloroplasts, vacuoles and mitochondria are arranged in the body. Animal cells have no cell wall, often a central body in the cytoplasm, and higher plant cells have no. The cell culture dish is a laboratory dish for culturing microorganisms or cells, and consists of a flat disc-shaped bottom and a cover, and is generally made of glass or plastic. The culture dish materials are basically divided into two types, mainly plastic and glass, and glass can be used for plant materials, microorganism culture and animal cell adherent culture;
The culture dish is required to be cleaned when the next culture is carried out after the use of the cell culture dish is finished every time, otherwise, the accuracy of cell culture is affected, in the cleaning process, generally, aqueous solution and cleaning solution are added into the culture dish, after the cleaning solution is mixed with the aqueous solution, the culture dish is cleaned, but the inner side wall and the inner bottom wall of the culture dish are difficult to clean and wash when the culture dish is cleaned, and the cleaning solution is difficult to be quickly mixed with the aqueous solution when the culture dish is cleaned, so that the cleaning efficiency is low, and improvement is needed.
The present invention seeks to mitigate or at least alleviate such problems or disadvantages by providing a new or otherwise improved culture dish.
Disclosure of Invention
In view of one or more of the above-mentioned drawbacks or improvements of the prior art, the present invention provides a cell culture dish, which has the advantages of being capable of simultaneously cleaning and flushing the inner side wall and the inner bottom wall of the culture dish, and further promoting the mixing of the cleaning solution and the aqueous solution during the cleaning process to enhance the cleaning effect.
In order to achieve the above-mentioned object, the present invention provides a cell culture dish, comprising a culture dish having a bearing cavity therein, a driving member comprising a connecting frame detachably mounted on the culture dish, a set of discs detachably arranged thereon, a driving motor arranged on the set of discs, an output end of the driving motor penetrating into the set of discs, a driving rod detachably arranged on the output end of the driving motor, and a first bevel gear and a driving wheel sequentially and removably arranged on the driving rod;
The connecting seat is detachably arranged on the sleeve disc, a linkage rod is rotatably arranged in the connecting seat, a second bevel gear meshed with the first bevel gear is detachably arranged at one end of the linkage rod, and a cam is detachably arranged at the other end of the linkage rod;
The stirring and mixing component is detachably arranged on the sleeve disk, is meshed with the driving wheel, and can penetrate into a bearing cavity of the culture dish;
the liquid mixing component is detachably arranged on the culture dish, can move along with the stirring and mixing component when the stirring and mixing component moves, and is used for feeding liquid into the bearing cavity;
the culture dish comprises a cam, a linkage scraping member and a built-in scraping member, wherein the linkage scraping member is detachably arranged on the culture dish and is provided with a vibrating section and a scraping section connected to the vibrating section, the vibrating section is arranged below the cam, the cam can trigger the vibrating section to scrape the inner side wall of the culture dish when rotating, the built-in scraping member is detachably arranged in the culture dish and is arranged below the vibrating section of the linkage scraping member, the vibrating section is in contact with the vibrating section of the linkage scraping member when in an initial position, and the vibrating section can be triggered when the vibrating section of the linkage scraping member works so as to scrape the inner bottom wall of the culture dish.
As a further improvement of the invention, the stirring and mixing member comprises a top disk which is detachably arranged at the bottom of the sleeve disk, a stirring shaft is detachably arranged in the top disk, and the stirring shaft can penetrate into a bearing cavity of the culture dish;
The driven wheel is detachably arranged on the stirring shaft and is meshed with the driving wheel;
The stirring device comprises a stirring shaft, flood dragon blades, a plurality of groups of cross bars and a plurality of groups of cross bars, wherein the flood dragon blades are detachably arranged at the tail end of the stirring shaft and can penetrate into a bearing cavity of the stirring vessel, the cross bars are detachably arranged on the stirring shaft, a first inclined blade is detachably arranged at one end of each group of cross bars, a second inclined blade is detachably arranged at the other end of each group of cross bars, and the inclination directions of the first inclined blade and the second inclined blade are opposite.
As a further improvement of the invention, the liquid mixing component comprises a left vertical plate which is detachably arranged on the culture dish;
the left connecting shell is detachably arranged on the left vertical plate, and a left stabilizing rod is detachably arranged in the left connecting shell;
The left-handed screw rod is rotatably arranged in the left connecting shell, one end of the left-handed screw rod can penetrate out of the left connecting shell, and a driving gear sleeve is detachably arranged at one end of the left-handed screw rod penetrating out of the left connecting shell;
the linkage fluted disc is detachably arranged on the stirring shaft and is meshed with the driving toothed sleeve;
The left-handed ball base is slidably arranged in the left connecting shell, is penetrated by the left stabilizing rod and the left-handed screw rod, and is detachably provided with a left liquid mixing spray gun;
the right vertical plate is detachably arranged on the culture dish;
The right connecting shell is detachably arranged on the right vertical plate, and a right stabilizing rod is detachably arranged in the right connecting shell;
The right-handed screw rod is rotatably arranged in the right connecting shell, one end of the right-handed screw rod can penetrate out of the right connecting shell, and one end of the right-handed screw rod penetrates into the driving gear sleeve;
The right-handed ball base is slidably arranged in the right connecting shell and penetrated by the right stabilizing rod and the right-handed screw rod, and the right-handed liquid mixing spray gun is detachably arranged on the right-handed ball base.
As a further improvement of the invention, the cross-sectional circular area of the linkage fluted disc is four times that of the driving tooth sleeve, and in the initial state, the left-hand ball base is positioned at the left side of the left connecting shell and is attached to the inner wall of the left connecting shell, the right-hand ball base is positioned at the right side of the right connecting shell and is attached to the inner wall of the right connecting shell.
As a further improvement of the invention, the vibrating section of the linkage scraping member comprises a connecting base plate which is detachably arranged on the culture dish, a straight rod is slidably arranged in the connecting base plate, and the straight rod can penetrate into a bearing cavity of the culture dish;
The upper roller is rotatably arranged at the upper section of the straight rod and is positioned below the cam;
the lug plate is integrally arranged on the upper section of the straight rod, a sliding rod is detachably arranged on the lug plate, and the sliding rod can slide in the connecting substrate;
The first spring is sleeved on the sliding rod in a removable way;
The limiting block is detachably arranged at one end of the sliding rod penetrating out of the connecting substrate and is mutually attached to the connecting substrate; and the lower roller is rotatably arranged at the lower section of the straight rod.
As a further improvement of the invention, the first spring is positioned below the ear plate, the first spring is mutually attached to the bottom surface of the ear plate, the first spring is positioned above the connecting substrate, and the first spring is mutually attached to the top surface of the connecting substrate.
As a further improvement of the present invention, the upper roller has the same size as the lower roller, and the upper roller has a smaller size than the cam.
As a further improvement of the invention, the scraping section of the linkage scraping member comprises a scraping machine frame which is detachably arranged on the straight rod and surrounds the straight rod therein, and can penetrate into a bearing cavity of the culture dish;
The first scraping brushes are detachably arranged on the four outer side walls of the scraping machine frame respectively, the bristles of the first scraping brushes are respectively abutted against the four inner side walls of the culture dish, the first communicating grooves are respectively formed in the four outer side walls of the scraping machine frame, and a first scraping brush is arranged between every two first communicating grooves.
As a further improvement of the present invention, the built-in scraping member includes a guide bar, which is located below the scraping frame, one end of which is detachably disposed on an inner sidewall of the culture dish, the other end of which is detachably disposed with a connecting bar, and one end of which is detachably disposed on the other inner sidewall opposite to the one inner sidewall of the culture dish;
the scraping plate is slidably arranged on the guide rod, a connecting block is detachably arranged on the top surface of the scraping plate, an inclined surface is arranged on the connecting block, and when the scraping plate is positioned at the initial position, the highest point of the inclined surface is mutually abutted with the lower roller;
the second spring is sleeved on the guide rod in a removable way, one end of the second spring is mutually attached to the scraping plate, and the other end of the second spring is mutually attached to the connecting rod;
The plurality of groups of second scraping brushes are detachably arranged on the bottom surface of the scraping plate, the bristles of the plurality of groups of second scraping brushes respectively contact with the inner bottom wall of the culture dish, the plurality of groups of second communicating grooves are formed in the bottom surface of the scraping plate, the plurality of groups of second communicating grooves can penetrate the scraping plate from bottom to top, and a group of second scraping brushes are arranged between every two second communicating grooves.
As a further improvement of the invention, the cross-sectional circular area of the connecting rod is larger than that of the guide rod, the caliber of the second spring is smaller than that of the connecting rod, a first magnetic adsorption gasket is arranged on one side surface of the guide rod, a second magnetic adsorption gasket is arranged on one side surface of the connecting rod, the first magnetic adsorption gasket is adsorbed on one inner side wall of the culture dish, and the second magnetic adsorption gasket is adsorbed on the other inner side wall opposite to the one inner side wall of the culture dish.
In general, the above technical solutions conceived by the present invention have the beneficial effects compared with the prior art including:
1. According to the cell culture dish, the stirring and mixing component can be triggered simultaneously to stir and mix liquid and the linkage scraping component can move downwards and upwards to scrape, the stirring and mixing component is arranged to drive the driving wheel to rotate after the driving motor drives the driving rod to rotate so that the driving wheel drives the driven wheel to rotate, the driven wheel drives the dragon blade to rotate after the driven wheel rotates so as to mix and stir the aqueous solution and the cleaning liquid in the bearing cavity of the culture dish, and meanwhile, in the process of mixing and stirring, the mixed solution of the aqueous solution and the cleaning liquid can be driven to shake to strengthen the flushing effect on the inner bottom wall and the inner side wall of the culture dish, and the mixing and stirring effect on the aqueous solution and the cleaning liquid can be further improved through the first inclined blade and the second inclined blade which are arranged in an inclined direction.
2. According to the cell culture dish, the stirring and mixing component is integrally driven by the driving component to rotate through the arranged liquid mixing component, so that the stirring shaft rotates, the linkage fluted disc is sleeved on the stirring shaft, so that the linkage fluted disc can be triggered and started at the same time, the linkage fluted disc rotates to drive the whole driving fluted disc to rotate so as to drive the left-handed screw and the right-handed screw to rotate, and the left-handed screw and the right-handed screw are opposite in running direction, so that after the left-handed screw and the right-handed screw rotate at the same time, the left liquid mixing spray gun and the right liquid mixing spray gun are mutually close to or mutually far away, and the left liquid mixing spray gun and the right liquid mixing spray gun are started to spray cleaning liquid into the culture dish along the linear direction, so that the problem that the cleaning liquid needs to be mixed with an aqueous solution for a long time after the linkage fluted disc rotates can be avoided, and meanwhile, the mixing of the cleaning liquid and the aqueous solution can be promoted when the cleaning liquid is stirred by the stirring and mixing component.
3. According to the cell culture dish, after the cam rotates, the protruding section of the cam can push the upper roller to integrally move downwards when touching the upper roller, so that the vibrating section of the cam and the cam integrally generate linkage to drive the straight rod to move downwards, the straight rod is connected with the scraping machine frame, and the scraping machine frame integrally moves downwards, so that the first scraping brush and the four inner side walls of the culture dish scrape impurities on the four inner side walls of the culture dish, the straight rod and the lug plate move downwards, the first spring can be compressed, after the protruding section of the cam is separated from the upper roller, the first spring loses compression rebound to drive the scraping machine frame to integrally move upwards, the four inner side walls of the culture dish are continuously scraped, and in the scraping process of one side of the scraping machine frame, the cleaning liquid and the aqueous solution in the culture dish can be oscillated, so that the mixing effect of the cleaning liquid and the aqueous solution is further accelerated.
4. According to the cell culture dish, the lower roller can be driven to move downwards after the straight rod moves downwards through the arranged built-in scraping component, and the lower roller is in contact with the highest point of the inclined plane in the initial stage, so that the highest point of the inclined plane moves towards the lowest point after the lower roller moves downwards, the whole scraping plate moves rightwards to drive the second scraping brush to scrape the inner bottom wall of the culture dish, the second spring can be compressed due to the rightwards movement of the scraping plate, the second spring is made to rebound after the lower roller returns to enable the scraping plate to move leftwards to scrape the inner bottom wall of the culture dish again, and further mixing operation can be carried out on cleaning liquid and aqueous solution in the culture dish after the second spring moves leftwards and rightwards continuously so as to promote scraping operation of the inner bottom wall of the culture dish.
Drawings
FIG. 1 is a schematic diagram of the overall structure of a cell culture dish according to the invention;
FIG. 2 is a schematic diagram of the structure of the cell culture dish according to the invention from another perspective;
FIG. 3 is an exploded view of a cell culture dish according to the invention;
FIG. 4 is a front view of a cell culture dish according to the invention;
FIG. 5 is a schematic view of the structure of the present invention when the linked scraping element interferes with the built-in scraping element;
FIG. 6 is a schematic view of the structure of the entirety of the built-in scraping member of the present invention;
FIG. 7 is a schematic view of the overall structure of the ganged scraping element of the present invention;
FIG. 8 is a schematic view of a partial construction of a ganged scraping element of the present invention;
FIG. 9 is a schematic view of the overall structure of the liquid mixing member of the present invention;
FIG. 10 is a schematic view of the structure of the driving member and the stirring and mixing member of the present invention when combined with each other;
FIG. 11 is a schematic view of the structure of the present invention when the driving member and the agitating mixing member are combined with each other from another angle;
FIG. 12 is a schematic view of the overall structure of the stirring and mixing member of the present invention;
FIG. 13 is an enlarged view of the invention at A of FIG. 6;
Fig. 14 is an enlarged view of fig. 7B in accordance with the present invention.
In all the drawings, the same reference numerals refer to the same technical features, in particular to 1, a culture dish; 2, driving member, 21, connecting frame, 22, sleeve disk, 23, driving motor, 24, driving rod, 25, first bevel gear, 26, connecting seat, 27, linkage rod, 28, second bevel gear, 281, cam, 29, driving wheel, 3, stirring mixing member, 31, top disk, 32, stirring shaft, 33, driven wheel, 34, dragon's blade, 35, cross rod, 36, first inclined blade, 37, second inclined blade, 4, liquid mixing member, 41, left vertical plate, 42, left connecting casing, 43, left stable rod, 44, left screw rod, 45, linkage fluted disc, 46, driving toothed sleeve, 47, left rotary ball base, 48, left mixed liquid spray gun, 49, right vertical plate, 491, right connecting casing, 492, right stable rod, 493, right rotary screw rod, 494, right rotary ball base, 495, right mixed liquid spray gun, 5, scraping member, 51, connecting substrate, 52, 53, upper roller, 54, ear plate, 55, straight rod, 56, first spring, 57, 58, second spring, 58, first inclined plate, 59, second inclined plate, 61, 62, second inclined plate, 65, and inner scraping groove.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The terms "comprises," "comprising," and/or the like, as used herein, specify the presence of stated features, steps, operations, and/or components, but do not preclude the presence or addition of one or more other features, steps, operations, or components.
All terms (including technical and scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art unless otherwise defined. It should be noted that the terms used herein should be construed to have meanings consistent with the context of the present specification and should not be construed in an idealized or overly formal manner.
In the examples, shown in FIGS. 1-14, a cell culture dish is shown, wherein FIG. 1 is a schematic diagram of the overall structure of the cell culture dish of the present invention; FIG. 2 is a schematic diagram of the structure of the cell culture dish according to the invention from another perspective; FIG. 3 is an exploded view of a cell culture dish according to the invention; fig. 4 is a front view of the cell culture dish according to the present invention, fig. 5 is a schematic view of the structure of the linked scraping member according to the present invention when the linked scraping member according to the present invention is abutted against the built-in scraping member, fig. 6 is a schematic view of the structure of the whole linked scraping member according to the present invention, fig. 7 is a schematic view of the structure of the linked scraping member according to the present invention, fig. 8 is a schematic view of the structure of the whole liquid mixing member according to the present invention, fig. 10 is a schematic view of the structure of the driving member according to the present invention when the driving member and the stirring mixing member are combined with each other, fig. 11 is a schematic view of the structure of the driving member according to the present invention when the driving member and the stirring mixing member are combined with each other from another angle, fig. 13 is an enlarged view of fig. 6 at a point a, fig. 14 is an enlarged view of fig. 7 at a point B, which includes a culture dish 1 having a bearing cavity therein, driving member 2 including a connection frame 21 detachably mounted on the culture dish 1, a set of discs 22 detachably laid thereon, a driving motor 23 is laid on the set of discs 22, and an output end of the driving motor 23 can penetrate into the set of the driving member 22, a driving shaft 23 can be penetrated into the set of the driving member 22, a driving shaft 24 is detachably laid on the driving shaft 24, a set of the driving shaft is detachably and a driving shaft is detachably laid on the driving shaft is connected to the driving member is set on the driving shaft is set on a driving member, and is set on a driving member is set in a driving member is in a detachable way, and is set on a driving member is in a driving shaft is in a detachable a driving shaft is in a driving way, a driving gear is in a driving a driving way, a driving gear is in a driving device is in a driving a driving a is a is a, a second bevel gear 28 meshed with the first bevel gear 25 is detachably arranged on one end of the linkage rod 27, a cam 281 is detachably arranged on the other end of the linkage rod 27, the stirring and mixing member 3 is detachably arranged on the sleeve disc 22 and is meshed with the driving wheel 29, the stirring and mixing member can penetrate into a bearing cavity of the culture dish 1, the liquid mixing member 4 is detachably arranged on the culture dish 1 and can move along with the stirring and mixing member 3 when the stirring and mixing member 3 moves and is used for feeding liquid in the bearing cavity, the linkage scraping member 5 is detachably arranged on the culture dish 1 and is provided with a vibrating section and a scraping section connected to the vibrating section, the vibrating section is arranged below the cam 281, the vibrating section can be triggered to scrape the inner side wall of the culture dish 1 when the cam 281 rotates, the scraping member 6 is detachably arranged in the culture dish 1, the scraping member 5 is arranged below the linkage member 5, and the vibrating section can be triggered to scrape the inner bottom wall of the culture dish 1 when the vibrating section is arranged at the initial position of the linkage member 5, and the vibrating section can be triggered to scrape the inner bottom wall of the culture dish 1 when the vibrating section is in a vibrating section.
First, the principle of the whole driving member 2 is further explained, after the driving motor 23 is turned on, a worker can drive the driving rod 24 to rotate and simultaneously drive the first bevel gear 25 on the driving rod 24 to rotate, because the first bevel gear 25 can drive the second bevel gear 28 to rotate after rotating, so as to change the rotation in the vertical direction into the rotation in the horizontal direction, so as to drive the cam 281 to rotate, after the cam 281 rotates, the protruding section of the cam 281 contacts the upper roller 53, the whole upper roller 53 can be pushed to move downwards, when the protruding section of the cam 281 contacts the upper roller 53, the semicircular section of the cam 281 does not contact the upper roller 53, and meanwhile, when the driving rod 24 rotates, the driving wheel 29 can be synchronously driven to rotate, so that the driving wheel 29 drives the driven wheel 33 to rotate, and the whole stirring and mixing member 3 is linked.
The stirring and mixing member 3 is further explained by giving a more specific structure and construction to the whole stirring and mixing member 3, wherein the stirring and mixing member 3 comprises a top plate 31 which is detachably arranged at the bottom of the sleeve plate 22, a stirring shaft 32 which is detachably arranged in the top plate, and the stirring shaft 32 can penetrate into a bearing cavity of the culture dish 1, a driven wheel 33 which is detachably arranged on the stirring shaft 32 and is meshed with the driving wheel 29, a dragon blade 34 which is detachably arranged at the tail end of the stirring shaft 32 and can penetrate into the bearing cavity of the culture dish 1, and a plurality of groups of cross bars 35 which are detachably arranged on the stirring shaft 32, wherein a first inclined blade 36 is detachably arranged at one end of each group of cross bars 35, a second inclined blade 37 is detachably arranged at the other end of each group of cross bars 35, and the inclination directions of the first inclined blade 36 and the second inclined blade 37 are opposite;
The use principle of the whole stirring and mixing member 3 is further explained, after the driving motor 23 drives the driving rod 24 to rotate, the driving wheel 29 is driven to rotate, so that the driving wheel 29 drives the driven wheel 33 to rotate, after the driven wheel 33 rotates, the driven wheel 33 drives the dragon blade 34 to rotate, so as to mix and stir the aqueous solution and the cleaning solution in the bearing cavity of the culture dish 1, and the mixed solution of the aqueous solution and the cleaning solution can be driven to shake to enhance the flushing effect on the inner bottom wall and the inner side wall of the culture dish 1 in the mixing and stirring process of the aqueous solution and the cleaning solution in the tilting direction can be further increased through the first tilting blade 36 and the second tilting blade 37 which are arranged.
Next, a more specific structure and construction will be given to the whole of the liquid mixing member 4 for further explanation, the liquid mixing member 4 including a left standing plate 41 detachably mounted on the culture dish 1; a left connection housing 42 detachably mounted on the Zuo Li board 41, in which a left fixing rod 43 is detachably disposed; a left-hand screw 44 rotatably disposed in the left connection housing 42, one end of which is capable of penetrating from the left connection housing 42, a driving gear sleeve 46 detachably disposed on one end of the left-hand screw 44 penetrating from the left connection housing 42, a linkage gear disc 45 rotatably disposed in the stirring shaft 32 and engaged with the driving gear sleeve 46, a left-hand ball seat 47 slidably disposed in the left connection housing 42 and penetrated by the left-hand stabilizing rod 43 and the left-hand screw 44, on which a left-hand liquid mixing spray gun 48 is detachably disposed, a right riser 49 detachably disposed on the culture dish 1, a right connection housing 491 detachably disposed on the right riser 49, in which a right stabilizing rod 492 is detachably disposed, a right-hand screw 493 rotatably disposed in the right connection housing 491, one end of which is capable of penetrating from the right connection housing 492 into the driving gear sleeve 46, a right-hand ball seat 494 detachably disposed on the right connection housing 491, and the right-hand ball seat 491 detachably disposed on the right connection housing 491, and the right-hand ball seat 493 detachably penetrating from the right connection housing 491;
Next, the use principle of the whole liquid mixing component 4 is further explained, after the whole driving component 2 drives the stirring and mixing component 3 to rotate, so that the stirring shaft 32 rotates, the linkage fluted disc 45 is sleeved on the stirring shaft 32, so that the linkage fluted disc 45 can be triggered and started simultaneously, the linkage fluted disc 45 rotates to drive the whole driving gear sleeve 46 to rotate so as to drive the left-handed screw 44 and the right-handed screw 493 to rotate, and the running directions of the left-handed screw 44 and the right-handed screw 493 are opposite, so that after the left-handed screw 44 and the right-handed screw 493 rotate simultaneously, the left-handed and right-handed spray guns 48 and 495 are made to be close to or far away from each other, so that the left-handed spray gun 48 and the right-handed spray gun 495 spray the cleaning liquid into the culture dish 1 along the straight line direction, the problem that the cleaning liquid needs a long time to be mixed with the aqueous solution can be avoided, and meanwhile, the mixing of the cleaning liquid and the aqueous solution can be promoted when the cleaning liquid is stirred by the stirring and mixing component 3.
In some embodiments, in order to make the stroke of the linkage fluted disc 45 after one cycle of movement larger than the stroke of the driving gear sleeve 46 after one cycle of movement, and to make the left-handed ball seat 47 and the right-handed ball seat 494 move along a left-to-right direction at the beginning of movement, the cross-sectional circular area of the linkage fluted disc 45 is four times that of the driving gear sleeve 46, in the initial state, the left-handed ball seat 47 is located at the left side of the left connecting housing 42, and the left-handed ball seat 47 is attached to the inner wall of the left connecting housing 42, the right-handed ball seat 494 is located at the right side of the right connecting housing 491, and the right-handed ball seat 494 is attached to the inner wall of the right connecting housing 491.
The vibration section of the linkage scraping member 5 is further explained by giving a more specific structure and construction, wherein the vibration section of the linkage scraping member 5 comprises a connecting base plate 51 which is detachably arranged on the culture dish 1, a straight rod 52 which is slidably arranged in the connecting base plate, the straight rod 52 can penetrate into a bearing cavity of the culture dish 1, an upper roller 53 which is rotatably arranged on the upper section of the straight rod 52 and is positioned below a cam 281, an ear plate 54 which is integrally arranged on the upper section of the straight rod 52, a sliding rod 55 which is detachably arranged on the upper section and can slide in the connecting base plate 51, a first spring 56 which is detachably sleeved on the sliding rod 55, a limiting block 57 which is detachably arranged at one end of the sliding rod 55 penetrating out of the connecting base plate 51 and is mutually attached with the connecting base plate 51, and a lower roller 58 which is rotatably arranged on the lower section of the straight rod 52;
Next, the use principle of the vibration section of the linkage scraping member 5 is further explained, after the cam 281 rotates, the protruding section of the cam 281 contacts the upper roller 53 to push the whole upper roller 53 to move downwards, so that the vibration section of the linkage scraping member 5 and the whole cam 281 are linked to drive the straight rod 52 to move downwards, and the straight rod 52 is connected with the scraping machine frame 59, and can enable the whole scraping machine frame 59 to move downwards, so that the first scraping brush 591 and four inner side walls of the culture dish 1 scrape impurities on the four inner side walls of the culture dish 1, and the straight rod 52 and the ear plates 54 move downwards, so that the first spring 56 is compressed, after the protruding section of the cam 281 is separated from the upper roller 53, the first spring 56 loses compression rebound to drive the scraping machine frame 59 to move upwards so as to continuously scrape four inner side walls of the culture dish 1, and in the process of scraping the cleaning liquid on one side of the scraping machine frame 59, the culture dish 1 can be further mixed with the aqueous solution under the action of the cleaning liquid.
In some embodiments, more specifically, to enhance the stability of the first spring 56 during operation, the first spring 56 is located below the ear plate 54, and the first spring 56 is attached to the bottom surface of the ear plate 54, the first spring 56 is located above the connection substrate 51, and the first spring 56 is attached to the top surface of the connection substrate 51.
In some embodiments, more specifically, in order to enable the cam 281 to continuously touch the upper roller 53, the upper roller 53 is sized the same as the lower roller 58, and the upper roller 53 is sized smaller than the cam 281.
Next, a more specific structure and construction are given to the scraping section of the linkage scraping member 5 for further explanation, wherein the scraping section of the linkage scraping member 5 comprises a scraping machine frame 59 which is detachably mounted on the straight rod 52 and surrounds the straight rod 52 therein and can penetrate into a bearing cavity of the culture dish 1, a plurality of groups of first scraping brushes 591 which are respectively detachably mounted on four outer side walls of the scraping machine frame 59 and respectively provided with bristles which are respectively abutted against four inner side walls of the culture dish 1, and a plurality of groups of first communicating grooves 592 which are respectively opened on the four outer side walls of the scraping machine frame 59 and are respectively provided with a group of first scraping brushes 591 between every two first communicating grooves 592;
Next, explanation will be further made on the scraping sections of the linkage scraping member 5, since the bristles provided by the first scraping brushes 591 on the four outer side walls of the scraper frame 59 are in contact with the four inner side walls of the culture dish 1, the scraping operation of the first scraping brushes 591 on the four inner side walls of the culture dish 1 can be enhanced when the scraper frame 59 moves down and up, and the inner wall surfaces of the scraper frame 59 and the outer wall surfaces thereof can be communicated with each other when mixing liquid is performed by the opened plurality of groups of first communicating grooves 592.
The scraping section of the built-in scraping member 6 is further explained by giving a more specific structure and construction, the built-in scraping member 6 comprises a guide rod 61, a second spring 64, a plurality of groups of second scraping brushes 65, a plurality of groups of connecting brushes 66, a plurality of groups of connecting brushes and a plurality of groups of connecting brushes, wherein the guide rod 61 is positioned below the scraping frame 59, one end of the guide rod is detachably arranged on one inner side wall of the culture dish 1, the other end of the guide rod is detachably arranged on the other end of the guide rod, one end of the connecting rod 62 is detachably arranged on the other inner side wall opposite to the one inner side wall of the culture dish 1;
Next, the use principle of the whole built-in scraping member 6 will be further explained, when the straight rod 52 moves down, the lower roller 58 can be driven to move down, since the lower roller 58 is in contact with the highest point of the inclined surface 68 at the beginning, the lower roller 58 moves from the highest point of the inclined surface 68 to the lowest point after moving down, so that the scraping plate 63 moves right wholly, so as to drive the second scraping brush 65 to scrape the inner bottom wall of the culture dish 1, and since the scraping plate 63 moves right can compress the second spring 64, after the lower roller 58 is retracted, the second spring 64 is made to rebound to move the scraping plate 63 leftwards to scrape the inner bottom wall of the culture dish 1 again, and after the second spring 64 moves continuously right and left, further mixing operation can be performed on the cleaning liquid and the aqueous solution in the culture dish 1, so as to promote the scraping operation of the bottom wall in the culture dish 1.
In some embodiments, in order to further facilitate the integral installation of the built-in scraping member 6 on the inner side wall of the culture dish 1, the cross-sectional circular area of the connecting rod 62 is larger than the cross-sectional circular area of the guiding rod 61, the caliber of the second spring 64 is smaller than the caliber of the connecting rod 62, a first magnetic force adsorption pad is arranged on one side surface of the guiding rod 61, a second magnetic force adsorption pad is arranged on one side surface of the connecting rod 62, the first magnetic force adsorption pad is adsorbed on one inner side wall of the culture dish 1, the second magnetic force adsorption pad is adsorbed on the other inner side wall opposite to the inner side wall of the culture dish 1, and the magnetic sheets opposite to the magnetism of the guiding rod 61 and the connecting rod 62 are arranged on the inner side wall surface of the culture dish 1, so that the guiding rod 61 and the connecting rod 62 are attracted to the inner side wall of the culture dish 1 through the principle of opposite phase attraction.
In summary, through the driving component 2 that sets up, can trigger stirring mixing component 3 simultaneously and stir the mixed solution and link scraping component 5 and carry out the scraping operation that reciprocates, through stirring mixing component 3 that lays, after driving motor 23 drives actuating lever 24 and rotates, with drive action wheel 29 to make driving wheel 29 drive driven wheel 33 rotate, after driven wheel 33 rotates, so that driven wheel 33 drives flood dragon leaf 34 and rotates, in order to carry out the mixed stirring operation to the aqueous solution in the cavity of culture dish 1 and washing liquid, and can drive the mixed solution of aqueous solution and washing liquid and shake in the in-process of mixing stirring simultaneously, at the first tilt leaf 36 of laying and second tilt leaf 37 again can carry out the mixed stirring of incline direction in order to further increase the mixed stirring effect to aqueous solution and washing liquid through laying, through the mixed liquid component 4 that lays, after driving component 2 is whole to drive stirring mixing component 3 and rotate, so that stirring shaft 32 rotates, because stirring shaft 45 is the cover is located on stirring shaft 32, so that the left and right sides 45 are driven by the mutual rotation of the screw 45 and the opposite to be driven screw 4944, and the screw 4944 is rotated simultaneously, the opposite to the screw 45 is driven to rotate and is moved in opposite directions of the screw 4944, and the screw 4944 and the screw rod is rotated simultaneously and the screw rod is rotated and the opposite to rotate 45 and is rotated and is far away from the screw rod 45, the left and right liquid-mixing spray guns 48 and 495 are opened so that the left and right liquid-mixing spray guns 48 and 495 spray the cleaning liquid into the culture dish 1 along the straight line direction, the problem that the cleaning liquid needs to be released for a long time to be mixed with the aqueous solution by a single point can be avoided, meanwhile, the mixing of the cleaning liquid and the aqueous solution can be promoted when the cleaning liquid is stirred by the stirring and mixing component 3, the upper roller 53 can be pushed to move downwards integrally by the distributed linkage scraping component 5 when the protruding section of the cam 281 contacts the upper roller 53 after the cam 281 rotates, so that the vibrating section of the linkage scraping component 5 and the cam 281 integrally generate linkage to drive the straight rod 52 to move downwards, the straight rod 52 is connected with the scraping machine frame 59 and the scraping machine frame 59 integrally moves downwards so that the first scraping brush 591 and the four inner side walls of the culture dish 1 perform operation, to scrape the impurities on the four inner side walls of the culture dish 1, as the straight rod 52 and the ear plate 54 move downwards, the first spring 56 can be compressed, after the protruding section of the cam 281 is separated from the upper roller 53, the first spring 56 loses compression rebound to drive the scraping machine frame 59 to move upwards as a whole so as to continuously scrape the four inner side walls of the culture dish 1, in the process of scraping the scraping machine frame 59 next, the cleaning liquid and the aqueous solution in the culture dish 1 can vibrate again to further accelerate the mixing action of the cleaning liquid and the aqueous solution, through the built-in scraping member 6, the lower roller 58 can be driven to move downwards after the straight rod 52 moves downwards, as the lower roller 58 is in contact with the highest point of the inclined surface 68 in the initial stage, the highest point of the inclined surface 68 moves towards the lowest point after the lower roller 58 moves downwards, so that the scraping plate 63 moves to the right integrally to drive the second scraping brush 65 to scrape the inner bottom wall of the culture dish 1, as the scraping plate 63 moves to the right to compress the second spring 64, after the lower roller 58 retreats, the second spring 64 rebounds to move the scraping plate 63 leftwards to scrape the inner bottom wall of the culture dish 1 again, and after the second spring 64 moves continuously right to left, the cleaning solution and the water solution in the culture dish 1 can be further mixed to promote the scraping operation of the bottom wall in the culture dish 1.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (5)
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| CN202411837902.0A CN119286649B (en) | 2024-12-13 | 2024-12-13 | A cell culture dish |
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| CN202411837902.0A CN119286649B (en) | 2024-12-13 | 2024-12-13 | A cell culture dish |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20130003266A (en) * | 2011-06-30 | 2013-01-09 | (주)오디에이테크놀로지 | Apparatus for washing inside of shopping cart |
| CN213826257U (en) * | 2020-07-22 | 2021-07-30 | 李灿阳 | Chemical barrel cleaning device for chemical industry |
| CN118698949A (en) * | 2023-03-27 | 2024-09-27 | 安徽朝西里工业设计有限公司 | A cleaning device |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101546760B1 (en) * | 2014-12-05 | 2015-08-24 | 주식회사 멈스전자 | Apparatus for food waste disposal cleaning hole of stirring net using water jet |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20130003266A (en) * | 2011-06-30 | 2013-01-09 | (주)오디에이테크놀로지 | Apparatus for washing inside of shopping cart |
| CN213826257U (en) * | 2020-07-22 | 2021-07-30 | 李灿阳 | Chemical barrel cleaning device for chemical industry |
| CN118698949A (en) * | 2023-03-27 | 2024-09-27 | 安徽朝西里工业设计有限公司 | A cleaning device |
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Effective date of registration: 20250822 Address after: No.20, Chazhong Road, Taijiang District, Fuzhou City, Fujian Province Patentee after: THE FIRST AFFILIATED HOSPITAL OF FUJIAN MEDICAL University Country or region after: China Address before: 350000 Fujian Province, Fuzhou City, Cangshan District, Jianxin Town, Jinzhou North Road No. 33, Building 3, Room 101 Patentee before: Fuzhou bavis Biotechnology Co.,Ltd. Country or region before: China |