CN119144416A - Fermentation device for microbial strain - Google Patents
Fermentation device for microbial strain Download PDFInfo
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- CN119144416A CN119144416A CN202411368714.8A CN202411368714A CN119144416A CN 119144416 A CN119144416 A CN 119144416A CN 202411368714 A CN202411368714 A CN 202411368714A CN 119144416 A CN119144416 A CN 119144416A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
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- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
本发明涉及生物发酵技术领域，公开了一种微生物菌种用发酵装置，包括发酵罐与供气机构，供气机构设置于发酵罐的内部，供气机构包括转动管和移动管，多组转动管在发酵罐内部转动对发酵罐内部的发酵物料进行搅动，同时转动管从发酵罐的内底部和发酵罐的内侧面对发酵物料输送气体。本发明通过转动和搅动的管道对发酵罐内部的物料进行翻动，同时管道上均匀分布的喷头可持续为罐体内部的微生物菌种进行发酵所需气体供应；转动杆底部同步进行转动的翻动结构可对罐体底部的发酵物料进行翻动，由于微生物菌种发酵过程中会产生较多的水分，同时从底部翻动上来的物料也会收到管道喷头的气体供应，获得更好的发酵效果。
The present invention relates to the field of biological fermentation technology, and discloses a fermentation device for microbial strains, including a fermentation tank and an air supply mechanism, wherein the air supply mechanism is arranged inside the fermentation tank, and the air supply mechanism includes a rotating tube and a moving tube, and multiple groups of rotating tubes rotate inside the fermentation tank to stir the fermented material inside the fermentation tank, and at the same time, the rotating tubes convey gas to the fermented material from the inner bottom and the inner side of the fermentation tank. The present invention stirs the material inside the fermentation tank through a rotating and stirring pipeline, and at the same time, nozzles evenly distributed on the pipeline can continuously supply the gas required for the fermentation of the microbial strains inside the tank body; the stirring structure that rotates synchronously at the bottom of the rotating rod can stir the fermented material at the bottom of the tank body, because the microbial strains will produce more water during the fermentation process, and at the same time, the material stirred up from the bottom will also receive the gas supply from the pipeline nozzle, so as to obtain a better fermentation effect.
Description
Technical Field
The invention relates to the technical field of biological fermentation, in particular to a fermentation device for microbial strains.
Background
The fermentation production modes of microbial enzyme preparation strains are two, one is solid fermentation, and the other is liquid submerged fermentation. The solid state fermentation process is mainly used for commercial enzyme production of fungal origin, wherein amylase is produced by aspergillus oryzae and protease is produced by aspergillus oryzae and mucor, and the culture mode is simple but the operation condition is not easy to control, and most of enzymes are produced by submerged fermentation culture at present.
The prior art discloses an enzyme preparation microorganism strain liquid fermentation device and a fermentation process thereof (publication number is CN 112251338B), and the fermentation process carried out by the device solves the problems of influence on fermentation effect caused by insufficient stirring and uneven distribution of dissolved oxygen in the fermentation process, and can be carried out under the condition of not influencing the dissolved oxygen. However, the technical scheme still has the following technical problems that the solution oxygen concentration in the fermentation liquid is promoted to be improved through a stirring mode, but the actual air distribution condition is related to the arrangement mode of the atomizing nozzle, so that air required by microorganisms cannot be uniformly distributed to all positions of the fermentation material in the actual operation process.
Disclosure of Invention
The invention aims to provide a technical scheme, materials are stirred in a mode of pipeline stirring and lifting movement, meanwhile, a dynamic pipeline has the function of air injection and oxygen supplement, and meanwhile, the linked bottom overturning structure also avoids continuous soaking of the bottom materials in liquid generated by fermentation, so that the problems in the prior art are solved.
In order to achieve the above purpose, the invention adopts the following technical scheme:
A fermentation device for a microbial species, comprising:
the fermentation tank and the air supply mechanism are arranged in the fermentation tank, the air supply mechanism comprises a rotating pipe and a moving pipe, a plurality of groups of rotating pipes rotate in the fermentation tank to stir fermentation materials in the fermentation tank, and meanwhile, the rotating pipes convey air from the inner bottom of the fermentation tank and the inner side of the fermentation tank to the fermentation materials;
The movable pipe is movably arranged on the rotating pipe, and can reciprocate and move up and down while rotating the rotating pipe, and the movable pipe can convey gas to the fermentation material in the fermentation tank while vertically turning the fermentation material in the fermentation tank.
Preferably, the rotating pipe is arranged symmetrically, a bidirectional screw rod is rotatably arranged on the rotating pipe, a moving block is arranged on the bidirectional screw rod in a threaded manner, the moving pipe is horizontally arranged on the rotating pipe through the moving block, and the bidirectional screw rod rotates to drive the reciprocating lifting movement of the moving pipe when the rotating pipe rotates.
Preferably, the air supply mechanism comprises a rotating rod, a plurality of groups of turning plates are arranged at the bottom of the rotating rod through rotating seats, the turning plates continuously turn fermentation materials at the bottom of the fermentation tank, and the fermentation materials at the bottom are prevented from being soaked in liquid generated by fermentation all the time.
Preferably, the bottom of fermentation cylinder is the slope setting, be connected through the universal joint drive between dwang and the rotation seat, multiunit turning plate laminating fermentation cylinder inner bottom's inclined plane, the bottom of rotation seat is connected with the location bull stick, the location bull stick rotates to be connected in fermentation cylinder inner bottom.
Preferably, a driving motor is installed at the top of the fermentation tank, the driving motor drives a rotating rod to rotate, a driving gear ring is installed in the fermentation tank, a transmission gear meshed with the driving gear ring is installed on the bidirectional screw rod, and when the plurality of groups of rotating pipes rotate in the fermentation tank, the transmission gear drives the bidirectional screw rod to rotate so as to drive the reciprocating lifting of the moving pipes.
Preferably, the outside of multiunit rotation pipe is installed the retainer plate, the locating seat that is used for the retainer plate rotation to fix a position is installed to the medial surface of retainer plate.
Preferably, the bottom of dwang sets up to hollow pole, the bottom of hollow pole is connected with the mount pad that is used for multiunit rotation pipe to install, hollow pole is the intercommunication setting with multiunit rotation pipe, hollow pole is the intercommunication setting with the removal pipe, and evenly distributed has multiunit shower nozzle on rotation pipe and the removal pipe.
Preferably, the gas supply is carried out to multiunit pivoted tube and removal pipe through hollow pole, the bottom of mount pad is provided with first air feed connector, be provided with the second air feed connector on hollow pole and the removal pipe, through air feed hose intercommunication between hollow pole and the removal pipe, be provided with the recess of stepping down that is used for pivoted pole and air feed hose to step down on the removal pipe.
Preferably, the gas generator is further comprised, a gas inlet pipe is connected to a gas supply port of the gas generator, and the gas generator is connected to the first gas supply joint through the gas inlet pipe.
Preferably, the bottom of the fermentation tank is provided with a tank body bracket, the top of the fermentation tank is provided with a tank cover, and one side of the bottom of the fermentation tank is provided with a discharge port.
The fermentation device for the microbial strain has the technical effects and advantages that compared with the prior art, the fermentation device for the microbial strain has the following advantages:
1. According to the invention, materials in the fermentation tank are turned over through the rotating and stirring pipeline, and meanwhile, the evenly distributed spray heads on the pipeline can continuously supply gas required by fermentation of microorganism strains in the tank;
2. the stirring structure capable of synchronously rotating the bottom of the rotating rod can stir the fermentation material at the bottom of the tank body, and as more water is generated in the fermentation process of the microbial strains, the stirring structure prevents the material at the bottom from being soaked in the bottom liquid all the time, and meanwhile, the material which is turned up from the bottom can also receive the gas supply of the pipeline spray head, so that a better fermentation effect is obtained;
3. by matching the corresponding driving mode and the gas supply mode, the gas of the pipeline spray head can be continuously and uniformly distributed to all corners of the fermentation tank.
Drawings
FIG. 1 is a schematic diagram showing the overall structure of a fermentation apparatus for a microorganism strain of the present invention;
FIG. 2 is a schematic diagram showing the internal structure of a fermentation apparatus for a microorganism strain of the present invention;
FIG. 3 is an enlarged schematic view of the structure of FIG. 2A according to the present invention;
FIG. 4 is a schematic view showing the internal structure of the fermenter according to the present invention;
FIG. 5 is an enlarged schematic view of the structure of FIG. 4B according to the present invention;
FIG. 6 is a schematic diagram of the air supply mechanism of the present invention;
FIG. 7 is a second schematic diagram of the air supply mechanism of the present invention;
FIG. 8 is a third schematic view of the air supply mechanism of the present invention;
Fig. 9 is a schematic view of the structure of the rotating rod, hollow rod and moving tube of the present invention.
In the figure:
1. The fermentation tank, 2, a gas generator, 3, a gas supply mechanism, 11, a tank body bracket, 12, a tank cover, 13, a discharge port, 31, a rotating rod, 32, a hollow rod, 33, a rotating pipe, 34, a moving pipe, 35, a positioning ring, 36, a positioning seat, 37, a universal joint, 38, a rotating seat, 39, a turning plate, 310, a spray head, 311, an installation seat, 312, a first gas supply joint, 313, a gas inlet pipe, 314, a bidirectional screw rod, 315, a driving gear ring, 316, a transmission gear, 317, a moving block, 318, a second gas supply joint, 319, a positioning rotating rod, 320, a yielding recess, 321 and a gas supply hose.
Detailed Description
The subject matter described herein will now be discussed with reference to example embodiments. It is to be understood that these embodiments are merely discussed so that those skilled in the art may better understand and implement the subject matter described herein and that changes may be made in the function and arrangement of the elements discussed without departing from the scope of the disclosure herein. Various examples may omit, replace, or add various procedures or components as desired. In addition, features described with respect to some examples may be combined in other examples as well.
The invention provides a fermentation device for microorganism strains, as shown in fig. 1-9, comprising:
the fermentation tank 1 and the gas supply mechanism 3, wherein the gas supply mechanism 3 is arranged in the fermentation tank 1, the gas supply mechanism 3 comprises a rotating pipe 33 and a moving pipe 34, a plurality of groups of rotating pipes 33 rotate in the fermentation tank 1 to stir fermentation materials in the fermentation tank 1, and meanwhile, the rotating pipes 33 convey gas from the inner bottom of the fermentation tank 1 and the inner side of the fermentation tank 1 to the fermentation materials;
The moving pipe 34 is movably installed on the rotating pipe 33, the moving pipe 34 moves up and down while the rotating pipe 33 rotates, and the moving pipe 34 conveys gas to the fermentation material in the fermentation tank 1 while turning up and down the fermentation material in the fermentation tank 1.
The fermentation tank has the working principle that the material in the fermentation tank 1 is turned through the rotating and stirring pipeline, and the evenly distributed spray heads 310 on the pipeline can continuously supply gas required by fermentation of microorganism strains in the tank, the turning structure which synchronously rotates at the bottom of the rotating rod 31 can turn the fermentation material at the bottom of the tank, and the turning structure prevents the material at the bottom from being soaked in the bottom liquid all the time because more moisture is generated in the fermentation process of the microorganism strains, and meanwhile, the material turned up from the bottom can also receive the gas supply of the pipeline spray heads 310 to obtain better fermentation effect;
The rotary pipe 33 agitates and conveys gas, and the rotary pipe 33 in the fermentation device rotates in the fermentation tank 1 to agitate the fermentation materials in the fermentation tank so as to increase the mixing uniformity of the materials. Meanwhile, the rotary tube 33 is also responsible for conveying gas from the inner bottom and the inner side of the fermentation tank 1 to provide oxygen required by microbial fermentation, the dynamic turning and gas conveying of the moving tube 34 are carried out, and the moving tube 34 is arranged on the rotary tube 33 and moves up and down in a reciprocating manner along with the rotation of the rotary tube 33. The design ensures that the movable pipe 34 can turn the materials up and down in the fermentation tank 1, thereby further promoting the mixing of the materials and the uniform distribution of the gas;
The fermentation tank has the advantages of improving oxygen transmission efficiency, improving the oxygen transmission efficiency by the cooperative work of the rotating pipe 33 and the moving pipe 34, promoting the activity and the fermentation efficiency of microorganisms, enhancing the stirring and turning action of the rotating pipe 33 and the moving pipe 34, helping the uniform distribution of the materials in the fermentation tank 1, reducing dead angles, ensuring the uniformity and consistency of the fermentation process, optimizing the fermentation environment, improving the fermentation environment, reducing the deposition and layering phenomena of the materials in the fermentation process by the turning action, providing more suitable growth environment for the microorganisms, improving the equipment utilization rate, allowing the fermentation tank 1 to realize more efficient gas supply and material stirring without adding additional equipment, improving the utilization rate and economic benefit of the equipment, and being easy to operate and maintain.
As shown in fig. 4 and 5, in order to perform the movement driving of the rotating tube 33 by the rotating tube 33, the rotating tube 33 is symmetrically arranged, a bidirectional screw rod 314 is rotatably mounted on the rotating tube 33, a moving block 317 is threadably mounted on the bidirectional screw rod 314, the moving tube 34 is horizontally arranged on the rotating tube 33 by the moving block 317, and the bidirectional screw rod 314 rotates while the rotating tube 33 rotates to drive the reciprocating lifting movement of the moving tube 34.
Because the materials in the fermentation tank 1 can continuously generate moisture in the fermentation process, as shown in fig. 2 and 3, the air supply mechanism 3 comprises a rotating rod 31, a plurality of groups of turning plates 39 are arranged at the bottom of the rotating rod 31 through a rotating seat 38, and the turning plates 39 continuously turn the fermentation materials at the bottom of the fermentation tank 1, so that the fermentation materials at the bottom are prevented from being soaked in liquid generated by fermentation all the time.
As shown in fig. 2 and 4, in order to enable the water produced by fermentation to be discharged from the bottom of the fermentation tank 1 as soon as possible, the bottom of the fermentation tank 1 is inclined, the rotating rod 31 is in driving connection with the rotating seat 38 through the universal joint 37, the plurality of groups of turning plates 39 are attached to the inclined surface of the inner bottom of the fermentation tank 1, the bottom of the rotating seat 38 is connected with the positioning rotating rod 319, and the positioning rotating rod 319 is rotatably connected to the inner bottom of the fermentation tank 1. Specifically, as shown in fig. 2, fig. 4 and fig. 6, in order to drive the rotating tube 33 and the moving tube 34 at the same time, a driving motor is installed at the top of the fermenter 1, the driving motor drives the rotating rod 31 to rotate, a driving gear ring 315 is installed in the fermenter 1, a transmission gear 316 meshed with the driving gear ring 315 is installed on the bidirectional screw 314, and when the plurality of groups of rotating tubes 33 rotate in the fermenter 1, the transmission gear 316 drives the bidirectional screw 314 to rotate so as to drive the moving tube 34 to reciprocate.
Specifically, as shown in fig. 2 and 4, a positioning ring 35 is mounted on the outer side of the plurality of sets of rotating pipes 33, and a positioning seat 36 for positioning the positioning ring 35 in a rotating manner is mounted on the inner side surface of the positioning ring 35.
As shown in fig. 9, in order to supply gas to the rotating pipes 33 and the moving pipes 34, the bottom of the rotating rod 31 is provided with a hollow rod 32, the bottom of the hollow rod 32 is connected with a mounting seat 311 for mounting a plurality of groups of rotating pipes 33, the hollow rod 32 is communicated with the moving pipes 34, and a plurality of groups of spray heads 310 are uniformly distributed on the rotating pipes 33 and the moving pipes 34.
As shown in fig. 6 to 8, regarding a more specific connection structure for gas supply, a plurality of sets of rotating pipes 33 and moving pipes 34 are used for gas supply through a hollow rod 32, a first gas supply connector 312 is arranged at the bottom of the mounting seat 311, a second gas supply connector 318 is arranged on the hollow rod 32 and the moving pipes 34, the hollow rod 32 and the moving pipes 34 are communicated through a gas supply hose 321, and a yielding recess 320 for yielding the rotating rod 31 and the gas supply hose 321 is arranged on the moving pipes 34.
Specifically, as shown in fig. 1 and 2, the gas generator 2 is further included, a gas supply port of the gas generator 2 is connected to a gas inlet pipe 313, and the gas generator 2 is connected to a first gas supply joint 312 through the gas inlet pipe 313. Further, a tank body bracket 11 is arranged at the bottom of the fermentation tank 1, a tank cover 12 is arranged at the top of the fermentation tank 1, and a discharge outlet 13 is arranged at one side of the bottom of the fermentation tank 1.
In summary, the present invention has the combined effect of pipe agitation and gas supply, and the gas supply mechanism 3 inside the fermentation apparatus includes a rotary pipe 33 and a moving pipe 34. The rotating tube 33 rotates inside the fermenter 1, agitates the fermented material while transporting gas from the inner bottom and the inner side facing the material. The moving tube 34 is arranged on the rotating tube 33, can perform reciprocating lifting movement while rotating, realizes up-and-down turning of materials and gas conveying, is driven by a bidirectional screw rod 314, and is connected with the moving tube 34 through a moving block 317 arranged in a threaded manner, wherein the bidirectional screw rod 314 is arranged on the rotating tube 33. The bottom turning structure comprises a bidirectional screw rod 314, a bottom turning structure, a gas supply mechanism 3, a rotating rod 31, a plurality of groups of turning plates 39, a gas supply system, a gas supply joint and a gas supply hose, wherein the bottom of the bottom turning structure is used for driving the moving tube 34 to reciprocate, the bottom turning structure is used for driving the moving tube 31, the bottom of the gas supply mechanism 3 is provided with a plurality of groups of turning plates 39, the turning plates 39 continuously turn materials at the bottom of the fermentation tank 1 and prevent the materials from being soaked in fermentation liquid for a long time, the bottom of the fermentation tank 1 is obliquely arranged, the turning plates 39 are attached to the inclined surface, the effective turning of the materials is realized through driving connection of the rotating seat 38 and the universal joint 37, the driving motor and the transmission system are used for driving the rotating rod 31 through the driving gear ring 315 at the top of the fermentation tank 1 and the transmission gear 316 are meshed with the transmission gear 316 on the bidirectional screw rod 314, the rotation of the rotating tube 33 and the lifting of the moving tube 34 are realized, and the gas supply system is used for supplying the gas through the hollow rod 32.
The fermentation device has the advantages of uniform gas distribution, capability of realizing continuous and uniform supply of gas required by microbial strain fermentation in the fermentation tank 1 through pipeline stirring and the design of the spray head 310, avoidance of material soaking, capability of avoiding long-time material soaking caused by moisture generated in the fermentation process by the bottom turning structure, improvement of fermentation efficiency, contribution to improvement of fermentation efficiency of microbial strain due to the turning of the material and uniform distribution of the gas, better fermentation effect, structural optimization, optimization of device structure, improvement of operation stability and efficiency due to the symmetrical arrangement of the rotary pipe 33 and the movable pipe 34, communication design of the hollow rod 32 and cooperation of the positioning ring 35 and the positioning seat 36, and strong adaptability.
While the embodiments of the present invention have been described, the embodiments are not limited to the above-described embodiments, which are intended to be illustrative only and not limiting, and many forms can be made by those of ordinary skill in the art, given the benefit of this disclosure, within the scope of the present embodiments.
Claims (10)
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202411368714.8A CN119144416B (en) | 2024-09-29 | 2024-09-29 | Fermentation device for microbial strain |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202411368714.8A CN119144416B (en) | 2024-09-29 | 2024-09-29 | Fermentation device for microbial strain |
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| CN119144416A true CN119144416A (en) | 2024-12-17 |
| CN119144416B CN119144416B (en) | 2025-07-18 |
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| KR102202243B1 (en) * | 2020-09-02 | 2021-01-12 | 윤해용 | Method for recycling of oyster-shells using effective microorganism |
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