CN118949532B

CN118949532B - A tumor cell cluster sorting and filtering device for brain tumor experiments

Info

Publication number: CN118949532B
Application number: CN202411434674.2A
Authority: CN
Inventors: 王睿君; 高乃康; 何占彪; 任君浩; 包洪恩; 朝博; 韩立杰
Original assignee: Affiliated Hospital Of Inner Mongolia Medical University Cardiovascular Research Institute Of Inner Mongolia Autonomous Region
Current assignee: Affiliated Hospital Of Inner Mongolia Medical University Cardiovascular Research Institute Of Inner Mongolia Autonomous Region
Priority date: 2024-10-15
Filing date: 2024-10-15
Publication date: 2025-01-28
Anticipated expiration: 2044-10-15
Also published as: CN118949532A

Abstract

The invention discloses a tumor cell cluster sorting and filtering device for brain tumor experiments, relates to the field of tumor cell cluster sorting, and solves the problem that the sieve holes of the existing tumor cell cluster sorting and filtering device for brain tumor experiments are easily clogged when in use and it is difficult to sort and collect the clogged objects during the cleaning process, resulting in low sorting efficiency. The invention comprises a body, a filtering mechanism, a dredging mechanism and a conveying mechanism, wherein the filtering mechanism comprises a screening cylinder, the dredging mechanism comprises an air blowing pipe and a cam, and the conveying mechanism comprises a conveying pipe, a collecting frame and a collecting plate. The tumor cell cluster sorting and filtering device for brain tumor experiments is convenient for sorting and filtering the cell clusters by driving the screening cylinder to rotate, and at the same time, the air blowing pipe is linked to blow air downward to the top of the screening cylinder, driving the cam to vibrate the upper outer wall of the screening cylinder, and dredging the sieve holes, collecting the screened cell clusters through the collecting frame, and collecting the large-sized cells that are shaken off by the dredging mechanism through the collecting plate.

Description

Tumor cell mass sorting and filtering device for brain tumor experiments

Technical Field

The invention relates to the technical field of tumor cell mass sorting, in particular to a tumor cell mass sorting and filtering device for brain tumor experiments.

Background

Brain tumors are also called intracranial tumors, the etiology of which is unknown so far, and research experiments on the brain tumors are necessary. The tumor is generated from brain, meninges, pituitary gland, cranial nerve, cerebral blood vessel and embryo residual tissue, which is called primary intracranial tumor, and the malignant tumor from other viscera tissue of body is transferred into cranium, which is called secondary intracranial tumor. In the working process, a doctor of oncology often needs to sort and filter the tumor cell mass through a sorting and filtering device, namely, fresh tissue dispersion liquid is added into the sorting and filtering device to sort and filter the tumor cell mass. Because the size of large tissues such as adipose tissue, membranous tissue, connective tissue and the like in the tissue dispersion is hundreds of micrometers, and the size of tumor cell clusters in the tissue dispersion is generally tens of micrometers, the large-sized tissues often block the filter plates in the sorting and filtering device, and the sorting progress of the tumor cell clusters is affected.

The existing sorting and filtering device adopts a plane screen to screen, and cell clusters inside the screen holes are cleaned in a shaking mode and the like through the screen, but due to the influence of gravity, the cleaned cell clusters still move into the screen holes, so that the screen holes are blocked again, and the subsequent sorting efficiency is affected. Therefore, we propose a tumor cell mass sorting and filtering device for brain tumor experiments.

Disclosure of Invention

The invention aims to provide a tumor cell mass sorting and filtering device for brain tumor experiments, which is convenient for improving the sorting efficiency, reducing the blocking condition of sieve holes and simultaneously discharging and collecting the blocking materials, so as to solve the problems in the background technology.

In order to achieve the purpose, the invention provides the technical scheme that the tumor cell mass sorting and filtering device for brain tumor experiments comprises a machine body, a filtering mechanism, a dredging mechanism and a conveying mechanism, wherein two sides of the machine body are fixedly connected with a first side plate and a second side plate respectively, the filtering mechanism comprises a screening cylinder arranged in the machine body and is used for driving the screening cylinder to rotate so as to sort and filter internal cell masses, the dredging mechanism comprises a blowing pipe fixedly arranged between the first side plate and the second side plate, the blowing pipe is arranged above the screening cylinder and is downwards opened, a plurality of groups of cams are uniformly and fixedly connected onto the rotating rod and are used for linking the blowing pipe to blow out gas from the top end of the screening cylinder when the screening cylinder rotates, the cams are used for driving the rotating rod to vibrate the upper side outer wall of the screening cylinder, the conveying mechanism comprises a conveying pipe arranged in the screening cylinder, the blowing pipe is downwards opened, the opening is arranged at the bottom of the screening cylinder and is horizontally connected with the conveying cylinder, and the collecting disc is connected with the collecting disc through the opening, and the collecting disc is convenient to discharge the collected mass from the collecting disc.

Preferably, the filter mechanism further comprises a rotating disc rotationally connected with the second side plate, a plurality of groups of connecting rods are uniformly and fixedly connected to the side face of the rotating disc, a threaded ring is rotationally connected to the first side plate, a plurality of groups of connecting rods are far away from one end of the rotating disc and are fixedly connected with the threaded ring, a plurality of groups of limiting grooves which can be slidingly inserted with the inner wall of the connecting rod along the horizontal direction are uniformly formed in the outer wall of the screening cylinder, the outer wall of the conveying pipe is rotationally connected with a threaded disc which is in threaded connection with the inner wall of the threaded ring, and a driving piece used for driving the rotating disc to rotate is arranged on the machine body and is convenient for sorting and filtering tumor cell clusters.

Preferably, the conveying mechanism further comprises a moving table arranged on the side face of the machine body, one end of the conveying pipe is connected with a feeding hopper in a communicating manner, a plugging seat is fixedly connected to the feeding hopper, a plugging groove which is plugged with the plugging seat along the vertical direction is formed in the moving table, one side of the collecting frame, which is close to the first side plate, is fixedly connected with the side face of the moving table, the collecting frame penetrates through the first side plate and is connected with the first side plate in a sliding manner along the horizontal direction, one end of the conveying pipe can be plugged with the rotating disc in a rotating manner, so that the screened cell clusters can be conveniently input into the screening cylinder through the output port on the conveying pipe, and the screened tumor cell clusters can be collected through the collecting frame.

Preferably, the dredging mechanism further comprises a connecting pipe rotationally connected with the second side plate, the connecting pipe penetrates through the second side plate, one end of the connecting pipe is communicated with the air blowing pipe, an outer wall of the connecting pipe is fixedly connected with an outer tooth ring, one end of a rotating rod is fixedly connected with a driving gear meshed with the outer tooth ring, the driving piece is used for synchronously driving the connecting pipe to rotate, and an air blowing piece used for blowing air in the air blowing pipe in the rotating process is arranged on the connecting pipe, so that cells blocked in sieve holes of the sieving barrel are cleaned and dredged conveniently.

Preferably, the driving piece comprises a driving motor fixedly mounted on the machine body, the output end of the driving motor is coaxially and fixedly connected with a driving shaft, one end of the driving shaft is provided with a speed reducer, the output end of the speed reducer is coaxially and fixedly connected with a rotating disc, a first belt pulley is coaxially and fixedly connected with the driving shaft, a second belt pulley is coaxially and fixedly connected with the connecting pipe, and a transmission belt connected with the second belt pulley in a transmission manner is connected with the outer wall of the first belt pulley, so that the rotating disc and the connecting pipe are conveniently and synchronously driven to rotate.

Preferably, the air blowing piece comprises an air inlet pipe fixedly mounted on the machine body, one end of the connecting pipe is fixedly connected with a wind wheel, the outer wall of the wind wheel is rotationally connected with the inner wall of the air inlet pipe, and air is blown into the air blowing pipe conveniently in the rotation process of the connecting pipe.

Preferably, the bottom fixedly connected with grafting piece of collecting tray, the upside of conveyer pipe is seted up can with the butt joint groove that the grafting piece was pegged graft mutually along vertical direction, is convenient for carry out the dismouting to the collecting tray.

Preferably, the filter screen is fixedly connected in the air inlet pipe, so that the air is filtered and filtered to remove impurities, and the influence on internal cell components is avoided.

Preferably, the upper side of the machine body is hinged with a box door, so that the cleaning and maintenance operations and the like of the interior of the machine body are facilitated.

Compared with the prior art, the invention has the beneficial effects that:

1. The invention provides a tumor cell mass sorting and filtering device for brain tumor experiments, which solves the problems that the inside of a sieve hole is easy to be blocked when the existing tumor cell mass sorting and filtering device for brain tumor experiments is used, and the blocking object is difficult to sort and collect in the cleaning process, so that the sorting efficiency is low, the cell mass to be screened is conveniently input into a screening cylinder through an output port on a conveying pipe, the screening cylinder is driven to rotate, so that the cell mass in the inside is sorted and filtered, a linkage air blowing pipe blows air downwards to the top end of the screening cylinder when the screening cylinder rotates, and meanwhile, a rotating rod is driven to rotate, so that a cam vibrates the outer wall of the upper side of the screening cylinder to dredge the sieve hole, the screened tumor cell mass is collected through a collecting frame, and large-size cells which are shaken off by a dredging mechanism are collected through a collecting disc, so that the sorting and dredging efficiency is high.

2. The device compact structure, the function is comprehensive, each structure is convenient for dismouting and maintenance, strike the connecting rod through the cam, the effectual condition that directly causes screening section of thick bamboo to screening section of thick bamboo surface impact to damage has been avoided, the life of screening section of thick bamboo has been prolonged, also can make the shake frequency of screening section of thick bamboo upside each position more even through holistic shake of connecting rod simultaneously, the mediation in-process can be automatic select separately the jam and collect, avoid falling back to continue to mix in the dispersion and influence screening efficiency, the probability of follow-up emergence jam has been reduced.

Drawings

FIG. 1 is a schematic diagram of the overall structure of the present invention;

FIG. 2 is a schematic view of the internal structure of the machine body of the present invention;

FIG. 3 shows a driving device of the present invention part partial structure schematic diagram;

FIG. 4 is an enlarged view of area A of FIG. 3;

FIG. 5 is a diagram showing a mobile station pull-out configuration according to the present invention;

FIG. 6 is an exploded view of a partial structure of the conveyor mechanism of the present invention;

FIG. 7 is an enlarged view of area B of FIG. 6;

FIG. 8 is a partial sectional view of the dredging mechanism of the present invention;

FIG. 9 is an enlarged view of region C of FIG. 8;

FIG. 10 is a partial cross-sectional view of the filter mechanism of the present invention;

FIG. 11 is a schematic view of the screen drum of the present invention in a disassembled condition;

fig. 12 is an enlarged view of region D in fig. 11.

The device comprises a machine body 1-part, a first side plate 2-part, a second side plate 3-part, a filtering mechanism 4-part, a dredging mechanism 5-part, a 6-blowing pipe, a 7-rotating rod 8-cam, a 9-conveying mechanism 10-conveying pipe 11-collecting frame 12-collecting tray 13-outlet 14-rotating tray 15-connecting rod 16-threaded ring 17-limit groove 18-threaded tray 19-driving part 20-moving table 21-feeding hopper 22-inserting seat 23-inserting groove 24-connecting pipe 25-outer toothed ring 26-driving gear 27-blowing part 28-driving motor 29-driving shaft 30-speed reducer 31-first belt pulley 32-second belt pulley 33-driving belt 34-air inlet pipe 35-wind wheel 36-inserting block 37-inserting groove 38-filter screen 39-box door 40-sieving barrel.

Detailed Description

The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

Referring to fig. 1-12, a tumor cell mass sorting and filtering device for brain tumor experiments in the drawings comprises a machine body 1, a filtering mechanism 4, a dredging mechanism 5 and a conveying mechanism 9, wherein a box door 39 is hinged to the upper side of the machine body 1, a first side plate 2 and a second side plate 3 are fixedly connected to two sides of the machine body 1 respectively, the filtering mechanism 4 comprises a screening cylinder 40 installed in the machine body 1 and used for driving the screening cylinder 40 to rotate so as to sort and filter internal cell masses, the dredging mechanism 5 comprises an air blowing pipe 6 fixedly installed between the first side plate 2 and the second side plate 3, the air blowing pipe 6 is located above the screening cylinder 40 and is provided with a downward opening, a rotating rod 7 is connected between the first side plate 2 and the second side plate 3 in a rotating mode, a plurality of groups of cams 8 are uniformly and fixedly connected to the rotating rod 7 and are used for blowing air to the top end of the screening cylinder 40 in a linked mode when the screening cylinder 40 rotates, and meanwhile the rotating rod 7 is driven to rotate, so that the cams 8 vibrate the outer wall of the upper side of the screening cylinder 40 to dredge holes.

The conveying mechanism 9 comprises a conveying pipe 10 arranged inside a screening cylinder 40, a collecting frame 11 is slidably connected to the bottom of the machine body 1 along the horizontal direction, a collecting tray 12 is arranged on the upper side of the conveying pipe 10, a plug-in block 36 is fixedly connected to the bottom of the collecting tray 12, a butt-joint groove 37 capable of being plugged with the plug-in block 36 along the vertical direction is formed in the upper side of the conveying pipe 10, an output port 13 communicated with the inside of the screening cylinder 40 is formed in the lower side of the conveying pipe 10, and used for inputting cell clusters to be screened into the screening cylinder 40 through the output port 13 on the conveying pipe 10, collecting the screened tumor cell clusters through the collecting frame 11, and collecting large-size cells falling through the dredging mechanism 5 through the collecting tray 12.

The filter mechanism 4 further comprises a rotating disc 14 rotationally connected with the second side plate 3, a plurality of groups of connecting rods 15 are uniformly and fixedly connected to the side surface of the rotating disc 14, a threaded ring 16 is rotationally connected to the first side plate 2, one ends of the plurality of groups of connecting rods 15 far away from the rotating disc 14 are fixedly connected with the threaded ring 16, a plurality of groups of limiting grooves 17 which can be slidingly spliced with the inner wall of the connecting rods 15 along the horizontal direction are uniformly formed in the outer wall of the screening cylinder 40, a threaded disc 18 which is in threaded connection with the inner wall of the threaded ring 16 is rotationally connected to the outer wall of the conveying pipe 10, and a driving piece 19 for driving the rotating disc 14 to rotate is arranged on the machine body 1.

The conveying mechanism 9 further comprises a movable table 20 arranged on the side face of the machine body 1, one end of the conveying pipe 10 is connected with a feeding hopper 21 in a communicating manner, a plug-in seat 22 is fixedly connected to the feeding hopper 21, a plug-in groove 23 which is plugged with the plug-in seat 22 along the vertical direction is formed in the movable table 20, one side, close to the first side plate 2, of the collecting frame 11 is fixedly connected with the side face of the movable table 20, the collecting frame 11 penetrates the first side plate 2 and is connected with the first side plate 2 in a sliding manner along the horizontal direction, and one end of the conveying pipe 10 can be plugged with the rotating disc 14 in a rotating manner.

In this embodiment, before use, the threaded connection state between the rotating threaded disc 18 and the threaded ring 16 is released, the moving table 20 slides outwards to drive the feeding hopper 21, the conveying pipe 10 and the collecting frame 11 to move out of the machine body 1 together, one end of the limit groove 17 on the clean screening cylinder 40 is aligned with the connecting rod 15, the screening cylinder 40 is pushed into the threaded ring 16, the moving table 20 is pushed to the machine body 1, the collecting frame 11 is inserted into the machine body 1, the conveying pipe 10 is inserted into the screening cylinder 40, the rotating threaded disc 18 and the threaded ring 16 are in threaded connection, the installation of the screening cylinder 40 can be completed, and otherwise, the disassembling operation of the screening cylinder 40 can be performed.

Through the tissue dispersion liquid to be sorted into the feeding hopper 21, liquid flows into the screening cylinder 40 through the output port 13 at the bottom of the conveying pipe 10, is accumulated at the bottom of the inner wall of the screening cylinder 40, and drives the rotating disc 14 to slowly rotate through the driving piece 19, so that the connecting rod 15 drives the screening cylinder 40 to rotate together, the liquid falls into the collecting frame 11 at the bottom to be collected after being screened by the sieve holes in the screening cylinder 40, so that tumor cell clusters with smaller diameters are obtained, and large-diameter cells which cannot pass through the sieve holes remain in the screening cylinder 40.

It is worth noting that when the blocking situation occurs in the sieve holes, the blocking objects are located in the lower side area of the sieve holes as the sieve holes are rotated to the top end along with the rotation of the sieving barrel 40, the blocking objects can be blown out of the sieve holes from top to bottom through the air blowing pipe 6, and fall into the collecting tray 12 to be collected, meanwhile, the dredging mechanism 5 can drive the rotating rod 7 to rotate, so that the cam 8 can knock the outer wall of the connecting rod 15 rotating to the upper side position, the upper end of the sieving barrel 40 is driven to vibrate through the vibration of the connecting rod 15, the falling efficiency of the blocking objects in the upper side sieve holes is improved, the situation that the sieving barrel 40 is damaged due to the fact that the surface of the sieving barrel 40 is directly bumped through the cam 8 is effectively avoided, the service life of the sieving barrel 40 is prolonged, and meanwhile, shaking frequencies of all positions on the upper side of the sieving barrel 40 can be more uniform through the integral shaking of the connecting rod 15.

The screening section of thick bamboo 40 after the mediation is accomplished can continue to slowly rotate to the bottom and carry out subsequent separation screening operation, the effectual in-process that has guaranteed to select separately, the sieve mesh that has the relative mediation all the time is moved to the bottom and is selected separately the operation, separation efficiency is higher, simultaneously also can collect the large diameter cell of jam through collecting tray 12, avoid it to fall back to the dispersion inside again after the mediation and influence subsequent separation efficiency, after the separation is accomplished, with screw thread dish 18 roll out, pulling mobile station 20 can shift out organism 1 with collecting frame 11 and collecting hopper, the convenience is collected required cell, upwards extract collecting tray 12, make grafting piece 36 and butt joint groove 37 remove grafting and can take off collecting tray 12, upwards extract into hopper 21, release the grafting state of grafting seat 22 and butt joint groove 23, can wholly remove into hopper 21 and conveyer pipe 10, conveniently follow-up take out screening section of thick bamboo 40 and take out organism 1 and wash and change.

Embodiment 2 referring to fig. 1-12 for further description of embodiment 2, the dredging mechanism 5 in the illustration further includes a connecting pipe 24 rotatably connected to the second side plate 3, the connecting pipe 24 penetrates through the second side plate 3, one end of the connecting pipe 24 is communicated with the air blowing pipe 6, an outer gear ring 25 is fixedly connected to an outer wall of the connecting pipe 24, a driving gear 26 meshed with the outer gear ring 25 is fixedly connected to one end of the rotating rod 7, the driving member 19 is used for synchronously driving the connecting pipe 24 to rotate, and the air blowing member 27 for blowing air in the air blowing pipe 6 in the rotating process is arranged on the connecting pipe 24.

The driving piece 19 comprises a driving motor 28 fixedly installed on the machine body 1, the model number of the driving motor 28 is preferably YYHS-40, the output end of the driving motor 28 is coaxially and fixedly connected with a driving shaft 29, one end of the driving shaft 29 is provided with a speed reducer 30, the output end of the speed reducer 30 is coaxially and fixedly connected with the rotating disc 14, a first belt pulley 31 is coaxially and fixedly connected with the driving shaft 29, a second belt pulley 32 is coaxially and fixedly connected with the connecting pipe 24, and a transmission belt 33 in transmission connection with the second belt pulley 32 is connected with the outer wall of the first belt pulley 31.

In this embodiment, the driving motor 28 is started to drive the driving shaft 29 to rotate, after the speed of the speed reducer 30 is reduced, the rotating disc 14 can be driven to slowly rotate, so that the rotation sorting operation of the sieving cylinder 40 is realized, meanwhile, the driving shaft 29 drives the first belt pulley 31 to drive the driving belt 33 to rotate the second belt pulley 32, the second belt pulley 32 drives the connecting pipe 24 to rotate, and then the outer toothed ring 25 drives the driving gears 26 meshed around to rotate (the rotating rod 7 can be one or two symmetrically distributed on two sides of the air blowing pipe 6), so that the rotating rod 7 and the cam 8 are driven to rotate, the cam 8 can impact the connecting rod 15 rotating to the upper side position to enable the upper side of the sieving cylinder 40 to vibrate, air is blown into the connecting pipe 24 through the air blowing piece 27, the air is input into the air blowing pipe 6 through the connecting pipe 24, and the air can be blown down from the top of the sieving cylinder 40 from top to bottom to blow out blocked cells in the sieve holes to the collecting disc 12 for collection.

Embodiment 3 referring to fig. 1-12 for further description of embodiment 3, embodiment 1 is further described, the blowing member 27 in the drawing includes an air inlet pipe 34 fixedly mounted on the machine body 1, one end of the connecting pipe 24 is fixedly connected with a wind wheel 35, an outer wall of the wind wheel 35 is rotatably connected with an inner wall of the air inlet pipe 34, and a filter screen 38 is fixedly connected in the air inlet pipe 34.

In this embodiment, when the connecting pipe 24 rotates, the wind wheel 35 is driven to rotate, gas is sucked from the outside through the air inlet pipe 34 and blown to the air blowing pipe 6, the gas can be filtered and filtered through the filter screen 38, the influence on internal cell components is avoided, meanwhile, the box door 39 is provided with a one-way air exhaust grid, the internal gas is exhausted to the outside in a one-way, and the operation such as flushing and overhauling can be performed on the inside of the machine body 1 by opening the box door 39 at the top.

It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A tumor cell mass sorting and filtering device for brain tumor experiments, which is characterized by comprising:

The device comprises a machine body (1), wherein a first side plate (2) and a second side plate (3) are fixedly connected to two sides of the machine body (1) respectively;

Further comprises:

the filtering mechanism (4) comprises a screening cylinder (40) arranged in the machine body (1) and is used for sorting and filtering the internal cell mass by driving the screening cylinder (40) to rotate;

The dredging mechanism (5), dredging mechanism (5) including fixed mounting in blow-off pipe (6) between first curb plate (2) with second curb plate (3), blow-off pipe (6) are located screening section of thick bamboo (40) top, and the opening is downward, rotate between first curb plate (2) and second curb plate (3) and be connected with multiunit dwang (7), even fixedly connected with multiunit cam (8) on dwang (7) are used for linkage when screening section of thick bamboo (40) rotate blow-off gas downwards to the top of screening section of thick bamboo (40), drive simultaneously dwang (7) rotate, make cam (8) pair screening section of thick bamboo (40) upside outer wall vibrates, dredges the sieve mesh;

The conveying mechanism (9), conveying mechanism (9) including install in inside conveyer pipe (10) of screening section of thick bamboo (40), there is collection frame (11) bottom along horizontal direction sliding connection in organism (1), the upside of conveyer pipe (10) is equipped with collection dish (12), delivery port (13) that are linked together with screening section of thick bamboo (40) are seted up to the downside of conveyer pipe (10), be used for through delivery port (13) on conveyer pipe (10) to the interior input of screening section of thick bamboo (40) of cell mass that awaits the screening, collect through collection frame (11) the tumour cell mass that will screen out, collect through collection dish (12) will pass through the jumbo size cell that mediation mechanism (5) shakes down, the bottom fixedly connected with grafting piece (36) of collection dish (12), the upside of conveyer pipe (10) is offered and can be pegged graft with grafting groove (37) in vertical direction mutually;

The filtering mechanism (4) further comprises a rotating disc (14) which is rotationally connected with the second side plate (3), a plurality of groups of connecting rods (15) are uniformly and fixedly connected to the side surface of the rotating disc (14), a threaded ring (16) is rotationally connected to the first side plate (2), a plurality of groups of connecting rods (15) are far away from one end of the rotating disc (14) and fixedly connected with the threaded ring (16), a plurality of groups of limiting grooves (17) which can be in sliding connection with the inner wall of the connecting rod (15) along the horizontal direction are uniformly formed in the outer wall of the screening cylinder (40), a threaded disc (18) which is rotationally connected with the inner wall of the threaded ring (16) is rotationally connected to the outer wall of the conveying pipe (10), a driving piece (19) which is used for driving the rotating disc (14) is arranged on the machine body (1), a moving table (20) which is arranged on the side surface of the machine body (1) is further arranged, one end of the conveying pipe (10) is communicated with an inlet hopper (21), a plurality of limiting grooves (17) which can be in sliding connection with the inner wall of the connecting rod (15) along the horizontal direction, a connecting seat (22) is fixedly connected with the first side surface of the machine body (1), a connecting seat (20) is arranged on the machine body (2), a connecting seat (20) along the side surface of the machine body, and the collecting seat (20), the collecting frame (11) penetrates through the first side plate (2) and is connected with the first side plate (2) in a sliding mode along the horizontal direction, and one end of the conveying pipe (10) can be connected with the rotating disc (14) in a rotating and inserting mode.

2. The tumor cell mass sorting and filtering device for brain tumor experiments according to claim 1, wherein the dredging mechanism (5) further comprises a connecting pipe (24) rotationally connected with the second side plate (3), the connecting pipe (24) penetrates through the second side plate (3), one end of the connecting pipe (24) is communicated with the air blowing pipe (6), an outer wall of the connecting pipe (24) is fixedly connected with an outer tooth ring (25), one end of a rotating rod (7) is fixedly connected with a driving gear (26) meshed with the outer tooth ring (25), the driving piece (19) is used for synchronously driving the connecting pipe (24) to rotate, and the connecting pipe (24) is provided with an air blowing piece (27) for blowing air in the air blowing pipe (6) in the rotating process.

3. The device for sorting and filtering the tumor cell mass for the brain tumor experiments according to claim 2, wherein the driving piece (19) comprises a driving motor (28) fixedly installed on the machine body (1), an output end of the driving motor (28) is coaxially and fixedly connected with a driving shaft (29), one end of the driving shaft (29) is provided with a speed reducer (30), an output end of the speed reducer (30) is coaxially and fixedly connected with the rotating disc (14), a first belt pulley (31) is coaxially and fixedly connected with the driving shaft (29), a second belt pulley (32) is coaxially and fixedly connected with the connecting pipe (24), and a driving belt (33) in driving connection with the second belt pulley (32) is connected to the outer wall of the first belt pulley (31).

4. The device for separating and filtering tumor cell clusters for brain tumor experiments according to claim 2, wherein the blowing part (27) comprises an air inlet pipe (34) fixedly arranged on the machine body (1), one end of the connecting pipe (24) is fixedly connected with a wind wheel (35), and the outer wall of the wind wheel (35) is rotatably connected with the inner wall of the air inlet pipe (34).

5. The device for separating and filtering tumor cell clusters for brain tumor experiments according to claim 4, wherein a filter screen (38) is fixedly connected in the air inlet pipe (34).

6. The device for sorting and filtering tumor cell clusters for brain tumor experiments according to claim 1, wherein a box door (39) is hinged to the upper side of the machine body (1).