CN1188490C - Method for extracting plant solid slag to obtain anti-oxidant through self anaerobic fermentation and produced extract - Google Patents
Method for extracting plant solid slag to obtain anti-oxidant through self anaerobic fermentation and produced extract Download PDFInfo
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- CN1188490C CN1188490C CNB001031554A CN00103155A CN1188490C CN 1188490 C CN1188490 C CN 1188490C CN B001031554 A CNB001031554 A CN B001031554A CN 00103155 A CN00103155 A CN 00103155A CN 1188490 C CN1188490 C CN 1188490C
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- anaerobically fermenting
- solid slag
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- vegetables
- fruit
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The present invention discloses a method of preparing antioxidant, which comprises the steps of anaerobic fermentation of plant materials, separation of fermented liquid, collection of precipitated solid slag, extraction of the precipitated solid slag with proper solvent and desiccation of the extracted matter.
Description
Technical field
The present invention relates to a kind of method for preparing polyphenoils, especially relate to a kind of from the method for plant through the solid slag extraction polyphenoils of anaerobically fermenting.
Background technology
Increase vegetables and the picked-up of fruit and the prevention tool dependency of cancer and cardiovascular disorder.By inference, increasing vegetables may be relevant with its contained anti-oxidant nutritive ingredient with the benefit of fruit picked-up.General biological intravital antioxidant system and active oxygen species maintain an equilibrium state, when the activity in vivo hyperoxia, promptly can produce oxidative pressure, and cause various pathologies and aging.And contain many antioxidant in vegetables and the fruit, can in and oxygen radical, except that vitamins C, E and β carotene, flavonoid, anthocyanidin etc. also has the high anti-oxidation activity, so the natural anti-reflecting oxide in vegetables and the fruit is the good combination of various polyphenoils.
According to statistics, Taiwan has vegetables and the fruit waste more than 900,000 tons every year at least, causes serious environmental issue.Therefore, the decrement of vegetables and fruit waste and resource recycling are instant things.
Present vegetables and fruit waste mainly are through burying processing.Also utilize anaerobically fermenting to handle vegetables and fruit waste, deodorize, purify the natural pond oxygen that produced in its process then with as gas fuel, and with its solid-liquid waste residue as the natural organic fertilizer material.
Yet, do not have any document or patent to disclose and can utilize vegetables and fruit waste to prepare polyphenoils.
Summary of the invention
Main purpose of the present invention provides a kind of method for preparing polyphenoils, and it is to utilize anaerobically fermenting to handle, and is applicable to all plant materials, comprises vegetables and fruit waste and herbal medicine.
Another object of the present invention provides a kind of according to the made antioxidant cpd of the inventive method.
Embodiment
The present invention at first provides a kind of method for preparing polyphenoils, and it comprises the following steps:
(a) plant is carried out anaerobically fermenting;
(b) separate fermentation liquid, and collect solid slag throw out;
(c), and collect extract with the collected solid slag throw out of appropriate solvent extraction; And
(d) dry extract.
Be applicable to the plant material of fermentation step of the present invention, can be any plant and plant part, particularly knownly contain high anti-oxidation activity person, for example as the plants such as herbal medicine class plant, Hu Luobu or tomato of Chinese medicinal materials.Preferred plant origin is vegetables and fruit plant or herbal medicine class plant, and preferred plant origin is vegetables and fruit waste, can reach the utilization that waste reclaims.
The anaerobically fermenting of step (a) can carry out according to any way known in the art.For example, can carry out 3 to 4 days anaerobically fermenting reaction under in 20 to 60 ℃ the temperature, preferably under 35 or 55 ℃, carry out fermentation reaction.This fermentation step is preferably the anaerobically fermenting of second order segmentation, fs is to carry out fermentation reaction in general closed cell, subordinate phase is then carried out the anaerobically fermenting reaction in the methanation groove, for example, can carry out methanation reaction at the mixed bed formula tower shape reactor that contains the filling of polyvinyl alcohol polymerization carrier.
Residue behind fermentation reaction then carries out separating of solid slag and liquid.Generally speaking, can carry out according to the isolating operation steps of melt cinder of reaching well known in the art, it includes but not limited to via centrifugation, collects the throw out of lower floor.
The extraction of step (c) can be carried out with any suitable organic solvent and/or water, and wherein organic solvent is preferably methyl alcohol, ethanol, normal hexane or its mixture.
Can carry out the drying of step (d) with any method known in the art, it includes but not limited to lyophilize, spraying drying, blast drier drying and thermopnore drying, is preferably the use lyophilize.
According to the extract of the inventive method gained, in the low-density lipoprotein system, show to have significant resistance of oxidation.Therefore, the present invention further provides a kind of antioxidant cpd via the inventive method preparation.
Resistance to oxidation according to the prepared antioxidant cpd institute tool of the inventive method, can use method well known in the art to measure, for example: low-density lipoprotein peroxidation susceptibility assay method, linolenic acid oxidation-resistance assay method, reducing power assay method, removing α, the free radical energy amylograph of α-two bases-β-picryl hydrazine (DPPH), chelating ferrous ion energy amylograph, removing superoxide anion energy amylograph etc.
The prepared antioxidant cpd according to the present invention can be used in feed, food, makeup, pharmaceuticals, sanitas, organic fertilizer or seed soak solution.
Below, further specify exploitativeness of the present invention by embodiment, so that the technology of the present invention content is more concrete, rather than be used to limit to scope of the present invention.Those skilled in the art are based on the teaching of prior art and attainable many variations of the present invention and improvement all should belong to category of the present invention.
Embodiment
Embodiment 1: the anaerobically fermenting of vegetables and fruit waste
(1) the fruit and vegetable raw material is handled
To insert plastic cylinder by collected vegetables and fruit waste from fruit and vegetable market, with about 150 times of sharp mouth bamboo bar puncture vegetables and fruit waste.
(2) bacterium source
Take from the anaerobic pond bed mud of pig house, remove sandstone, and with after the allotment of 1000ppm calcium acetate with the filter mesh sieve of 2 and 30 sieve meshes, with the bacterium mud of this feathering as inoculation source.
(3) anaerobically fermenting is cultivated
Bacterial classification inoculation is gone in the cylinder, and covered and enclosed carries out anaerobically fermenting and cultivated 3 to 4 days under 35 or 55 ℃.
(4) methanation anaerobically fermenting
Anaerobically fermenting is cultivated the residue and the liquid of gained, immigration contains the mixed bed formula tower shape reactor of 3 centimeters cubical polyvinyl alcohol polymerization carriers filling, under 35 or 55 ℃, carried out methanation reaction 3 to 4 days, and promptly obtained vegetables and fruit waste through anaerobically fermenting.
Embodiment 2: the extraction of polyphenoils
Vegetables and fruit castoff slag through anaerobically fermenting, centrifugal (4,500rpm, 4 ℃) 15 minutes, take off a layer throw out (Gu slag) and be distributed into tubule, put into-70 ℃ of refrigerators lyophilize bottle of packing into after the freezing night, carried out the concentrating under reduced pressure drying 1～2 day, each is managed the solid slag of exsiccant and mixes, wear into fine powder with liquid nitrogen after, be distributed into tubule and be kept at-20 ℃ of refrigerators.Gu the slag fine powder extracts respectively with four kinds of extraction agents such as methyl alcohol, normal hexane, alcohol or the aqueous solution, first three is planted organic extractant solution thing and dries up with concentrating under reduced pressure or nitrogen, and the latter obtains dry extract with freeze concentration.
(1) organic extractant phase
Get each 1 gram of solid slag, extract respectively with 10 milliliters alcohol, methyl alcohol or normal hexane, earlier to organize masher to homogenize, again with 2,000g got supernatant liquor after under 4 ℃ centrifugal 10 minutes.Repeat above-mentioned extraction and centrifugation step totally 5 times, do not have color up to extract.Compile 6 times supernatant liquor, dry up with concentrating under reduced pressure or nitrogen at normal temperatures, last supernatant liquor dewaters with freeze drier, dry thing be stored in subzero 20 ℃ standby.
(2) water extraction
Get solid slag 1 gram,, homogenize,, under 000g, 4 ℃ the condition centrifugal 30 minutes, get supernatant liquor again with 25 to organize masher with 5 milliliters of extraction liquid 10mM Tris (pH 7.0) and a little polyethylene polypyrrole pyridine ketone.Repeat above-mentioned steps 6 times, do not have color up to extract.After the supernatant liquor of all water extraction liquids mixed, with the freeze drier dehydration, dry thing be stored in subzero 20 ℃ up to use.
Embodiment 3: the resistance to oxidation of low-density lipoprotein is responsive to be measured
(1) purifying of low-density lipoprotein
Get 30 milliliters of venous blood, inject the heparin tube contain 1.5 mg/ml EDTA-K3, with 3,000rpm and 4 ℃ were obtained blood plasma in centrifugal 15 minutes, separated low-density lipoprotein with two-section type ultra-high speed centrifuging (1.006＞d＞1.063 grams per milliliters) again.At first with 44,000rpm centrifugal 16 hours (d＜1.006 grams per milliliters) removes very low-density lipoprotein (VLDL, verylow-density lipoprotein) layer, then centrifugal again 20 hours (d＜1.063 grams per milliliters) gets low-density lipoprotein (LDL, low-density lipoprotein) layer.Low-density lipoprotein carries out low-density lipoprotein with 50mM phosphate buffer soln (pH 7.4) dialysis after 22 hours peroxidation susceptibility is inspected.
(2) the oxidation-sensitive degree of low-density lipoprotein is measured
The low-density lipoprotein of dialysing is diluted to the cholesterol concentration of 0.9 mg/ml, gets quantitative low-density lipoprotein and 25 μ M CuSO
4Cultivated 5 hours at 37 ℃, and measured the growing amount of MDA (malonyldialdehyde, mda) respectively at 0,30,60,70,80,90,100,120,150,180,240 and 300 minute.The Tricholroacetic Acid (15.2% that adds 100 μ L, w/v) with after the stopped reaction, get the trifluoracetic acid that quantitative supernatant liquor adds 1 milliliter (0.6%, w/v) place 100 ℃ of heating to insert cold water after 30 minutes and cool off, get the light absorption value of supernatant liquor survey, calculate the growing amount of MDA according to this at 532nm.
The mda growing amount of gained, the result through converting can obtain the resistance to oxidation result of different extracts in the low-density lipoprotein system, with I
50Be expressed as follows table:
I shown in the table
50Value representation suppresses the required extract concentration of LDL (being that MDA generates) 50%.By the result in the table as can be known, use the mixture of methanol extraction gained, have best resistance to oxidation.
| Extraction phase | I 50(mg/ml) |
| The aqueous solution | 130 |
| Alcohol | 28 |
| Methyl alcohol | 13 |
| Normal hexane | 24 |
Claims (15)
1, a kind of method for preparing polyphenoils comprises the following steps:
(a) plant is carried out anaerobically fermenting, described fermentation step is the anaerobically fermenting of second order segmentation, and the fs is to carry out fermentation reaction in closed cell, and subordinate phase is then carried out the anaerobically fermenting reaction in the methanation groove;
(b) separate fermentation liquid, and collect solid slag throw out;
(c), and collect extract with the collected solid slag throw out of appropriate solvent extraction; And
(d) dry extract.
2, the method for claim 1, wherein described plant is vegetables or fruit.
3, method as claimed in claim 2, wherein, described plant is the waste of vegetables or fruit.
4, the method for claim 1, wherein described plant is a herbal medicine.
5, the method for claim 1, wherein described methanation groove is the mixed bed formula tower shape reactor that contains the filling of polyvinyl alcohol polymerization carrier.
6, the method for claim 1, wherein described solvent is an organic solvent.
7, method as claimed in claim 6, wherein, described organic solvent is selected from the group of being made up of methyl alcohol, ethanol, normal hexane.
8, method as claimed in claim 7, wherein, described organic solvent is a methyl alcohol.
9, the method for claim 1, wherein described solvent is a water.
10, the method for claim 1, wherein described drying is lyophilize.
11, the method for claim 1, wherein the temperature of described anaerobically fermenting is 20 ℃ to 60 ℃.
12, method as claimed in claim 11, wherein, the temperature of described anaerobically fermenting is 35 ℃.
13, method as claimed in claim 11, wherein, the temperature of described anaerobically fermenting is 55 ℃.
14, a kind of antioxidant cpd, it is method preparation according to claim 1.
15, the purposes of mixture as claimed in claim 14 in feed, food, makeup, pharmaceuticals, sanitas, organic fertilizer or seed soak solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB001031554A CN1188490C (en) | 2000-03-17 | 2000-03-17 | Method for extracting plant solid slag to obtain anti-oxidant through self anaerobic fermentation and produced extract |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB001031554A CN1188490C (en) | 2000-03-17 | 2000-03-17 | Method for extracting plant solid slag to obtain anti-oxidant through self anaerobic fermentation and produced extract |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1314449A CN1314449A (en) | 2001-09-26 |
| CN1188490C true CN1188490C (en) | 2005-02-09 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNB001031554A Expired - Fee Related CN1188490C (en) | 2000-03-17 | 2000-03-17 | Method for extracting plant solid slag to obtain anti-oxidant through self anaerobic fermentation and produced extract |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105906398A (en) * | 2016-04-13 | 2016-08-31 | 齐鲁工业大学 | Method for preparing organic fertilizer through processing agriculture and forestry wastes by using organic solvent |
| CN108522854A (en) * | 2017-03-03 | 2018-09-14 | 重庆市阿陀利纳米技术发展有限公司 | A kind of nanometer of organic feed and preparation method thereof |
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