CN118749372A - A method and device for breeding golden retrievers using spores - Google Patents

Abstract

The invention discloses a method and device for breeding golden retriever by using spores, which relates to the field of plant breeding and comprises the following steps: S1, collecting and preserving spores; S2, sowing spores; S3, daily management and maintenance; S4, transforming sporophytes; S5, strengthening young sporophyte seedlings; S6, training seedlings; S7, planting under forests; the invention proposes an optimal sowing spore time and young sporophyte transplanting time, stipulates the specifications of seedling raising containers, and proposes measures to promote the transformation of sporophytes, and has the advantages of low cost, stable emergence and short cycle, etc.; after the antheridia and archegonium of the prothallus are produced, they are soaked in water and oscillated with appropriate amplitude and frequency, so as to accelerate fertilization and promote the transformation of sporophytes.

Description

A method and device for breeding golden retrievers using spores

Technical Field

The invention relates to the technical field of plant breeding, and in particular to a method and a device for breeding golden retrievers by utilizing spores.

Background Art

Cibotium barometz is a plant of the Cibotium genus of the Cibotaceae family, also known as yellow-haired dog and monkey hair head, and is a national second-level protected plant. Its rhizome can be used as medicine (also known as Cibotium barometz), which has the effects of removing rheumatism, nourishing the liver and kidneys, and strengthening the waist and knees. It has been included in the Chinese Pharmacopoeia. According to statistics from a certain big data platform for the traditional Chinese medicine industry, more than 120 kinds of Chinese patent medicines contain Cibotium barometz, and its usage in 2022 is 574 tons, with a stable and large market demand. In recent years, as Cibotium barometz was out of stock in the market, in order to solve the problem of raw material demand of pharmaceutical companies, it is urgent to carry out artificial cultivation of Cibotium barometz.

For this reason, we provide a method and device for breeding golden retrievers using spores to solve the above problems.

Summary of the invention

In view of the problems existing in the above-mentioned prior art, the present invention provides a method and device for breeding golden dogs using spores. The present invention proposes the optimal spore sowing time and young sporophyte transplanting time, stipulates the specifications of seedling containers, and proposes measures to promote sporophyte transformation. The method has the advantages of low cost, stable seedling emergence and short cycle.

In order to achieve the above object, the present invention adopts a method for breeding golden retrievers using spores, comprising the following steps:

S1. Spore collection and storage: collect mature spores of golden dog and store them in a refrigerator for later use;

S2. Spore sowing: After disinfecting the substrate, moisten it with water and spread it flat in the seedling box. Make the spores into a suspension and spray it on the surface of the substrate;

S3. Daily care: After sowing spores, place the seedling box in the culture room, wait for the prothallus to germinate, and perform daily care until the sporophyte is transplanted;

S4, sporophyte transformation: After the prothallus matures, it produces antheridia and archegonium, which are soaked in water and placed in a shaker for shaking to promote sporophyte transformation, and wait for the sporophyte to grow into young sporophytes;

S5. Young sporophyte seedlings: When the young sporophyte grows to 4-5 leaves and the plant height is more than 2 cm, the young sporophyte is transplanted into the greenhouse for seedling cultivation;

S6. Seedling training: Seedling training should be carried out half a month before planting under the forest;

S7. Planting under the forest: Seedlings of one year or older are planted under the forest after being hardened.

As a further optimization of the above scheme, the method for collecting and preserving spores in S1 is as follows: collect dark brown leaves containing mature and uncracked golden dog spores, wash them with clean water to prevent mixing with other plant spores, place them in a dry place with newspaper as a pad, wait for the sporangium to completely crack, sieve them with a 200-mesh sieve to obtain pure spores, put the pure spores into a ziplock bag and store them in a refrigerator for later use.

As a further optimization of the above scheme, the spore sowing method in S2 is to sow spores in early November every year, prepare the matrix and spore suspension 24 hours in advance, sterilize the matrix with an autoclave, moisten and spread it in a seedling box, soak the spores in a suspension for 24 hours, sow the spores 24 hours later, and sow the spores by spraying. Shake the spray pot each time to spray the spores evenly in the seedling box.

As a further optimization of the above scheme, the matrix is made of peat, coconut husk, fir sawdust, vermiculite and perlite, and the fir sawdust has a diameter of 0-6 mm.

As a further optimization of the above solution, the seedling box is a container made of PP material with a sealing lid, which is 22.3 cm long, 15.3 cm wide and 7.9 cm high.

As a further optimization of the above scheme, the daily maintenance method in S3 is to control the indoor temperature of the culture room at about 25°C, the light intensity is 2000-3000Lx, the light cycle is 12h/d, the seedling box is kept moist, and after spore germination, Huaduoduo No. 1 water-soluble fertilizer is sprayed every week, and the sealed lid of the seedling box is opened for ventilation for 2h every day.

As a further optimization of the above scheme, the method for sporophyte transformation in S4 is to soak the prothallus antheridia and archegonials after they are produced, so as to accelerate fertilization and promote sporophyte transformation. The amount of water added is just enough to cover the prothallus, and the prothallus is placed on a shaker for shaking with an amplitude of 20 mm and a frequency of 200 rpm, and the shaking is repeated for 30 minutes.

As a further optimization of the above scheme, the method for strengthening the young sporophytes in S5 is to transplant them into a non-woven container bag in a greenhouse in February and March of the second year when the young sporophytes grow to 4-5 leaves and the plant height is more than 2 cm, cover them with a layer of drip-free mulch to keep warm and moist, and spray 500 times diluted carbendazim to prevent diseases. Remove the drip-free mulch after half a month.

The present invention discloses a device for breeding golden dogs using spores, which is applied to the method for breeding young spore seedlings of golden dogs as described in any one of the above items, comprising a sealing cover arranged on a seedling box, the sealing cover being provided with a vent, a sealing plate being movably arranged in the vent, a rotating shaft being fixedly arranged at one end of the sealing plate, the rotating shaft being rotatably connected in the vent, one side of the lower end of the sealing plate being movably fitted with a limiting block, and the other side being fixedly connected with a tension spring, the lower end of the tension spring being fixed on the inner wall of the vent, and the limiting block being fixedly connected to the inner wall of the vent.

As a further optimization of the above scheme, the sum of the tension of the tension spring and the gravity of the sealing plate is equal to the difference between the pressure at the highest threshold temperature inside the seedling box and the pressure at the highest threshold temperature outside the seedling box.

The method and device for breeding golden retrievers using spores of the present invention have the following beneficial effects:

1. A method for breeding golden dog using spores of the present invention, the present invention proposes the best spore sowing time and young sporophyte transplanting time, stipulates the specifications of seedling raising containers, and proposes measures to promote sporophyte transformation, which has the advantages of low cost, stable seedling emergence and short cycle;

2. A method for breeding golden dogs using spores of the present invention comprises the following steps: after the spermatozoa and archegonium of the prothallus are produced, the prothallus is soaked in water and vibrated with a suitable amplitude and frequency, which can accelerate fertilization and promote sporophyte transformation;

3. The device for breeding golden dog using spores of the present invention can just push the sealing plate upward and open it when the temperature inside the seedling box exceeds 28°C, thereby achieving pressure relief and heat dissipation, ensuring that the temperature inside the seedling box does not exceed 28°C, achieving the purpose of automatic management and maintenance, without the need for frequent manual care, and having a simple structure, without the need for electronic equipment such as temperature sensors, and having low cost, thereby promoting the growth of young spore seedlings of golden dog;

4. The device for breeding golden retrievers using spores of the present invention, the sealing cover provided in the present invention can also be used to cultivate other seedlings, and a tension spring with appropriate tension and a sealing plate with appropriate gravity can be used according to the growth conditions of other seedlings, and the adaptive adjustment has a wide range of applications.

With reference to the following description and drawings, a specific embodiment of the present invention is disclosed in detail, indicating the manner in which the principles of the present invention can be adopted. It should be understood that the scope of the embodiments of the present invention is not limited thereby, and within the spirit and scope of the appended claims, the embodiments of the present invention include many changes, modifications and equivalents.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG1 is a diagram showing the steps of a method for breeding golden retrievers using spores according to the present invention;

FIG2 is a top view of the sealing cover of the present invention;

FIG. 3 is a cross-sectional view of the sealing cover of the present invention.

In the figure: 1. Sealing cover; 2. Ventilation port; 3. Sealing plate; 4. Rotating shaft; 5. Tension spring; 6. Sealing ring; 7. Limit block.

DETAILED DESCRIPTION

In order to make the purpose, technical scheme and advantages of the present invention more clear, the present invention is further described in detail below through the accompanying drawings and embodiments. However, it should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the scope of the present invention.

It should be noted that when an element is referred to as being "disposed on, provided with" another element, it may be directly on the other element or there may also be a central element. When an element is considered to be "connected, connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. "Fixed connection" means a fixed connection. There are many ways of fixed connection, which are not within the scope of protection of this article. The terms "vertical", "horizontal", "left", "right" and similar expressions used in this article are for illustrative purposes only and do not represent the only implementation method.

Unless otherwise defined, all technical and scientific terms used herein have the same meanings as those commonly understood by those skilled in the art to which the present invention belongs. The terms used in the specification are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and/or" used herein includes any and all combinations of one or more related listed items.

Example 1, please refer to Figure 1 of the specification, the present invention provides a technical solution: a method for breeding golden dogs using spores, including: the purpose is to provide a complete technical solution for large-scale breeding of golden dogs. The specific steps are as follows:

1. Spore collection and storage: Collect mature spores of golden dog and store them in the refrigerator for later use.

2. Sowing spores: After disinfecting the substrate, moisten it with water and spread it flat in the seedling box. Make the spores into a suspension and spray it on the surface of the substrate.

3. Daily care: After sowing spores, place the seedling box in the culture room, wait for the prothallus to germinate, and carry out daily care until the sporophyte is transplanted.

4. Sporophyte transformation: After the prothallus matures, it produces antheridia and archegonia. It is soaked in water and placed in a shaker for shaking to promote sporophyte transformation. Wait for the sporophyte to grow into young sporophytes.

5. Strengthening young sporophytes: When the young sporophytes grow to 4-5 leaves and the plant height is more than 2 cm, transplant the young sporophytes into the greenhouse for seedling cultivation.

6. Seedling training: Seedling training should be carried out half a month before planting under the forest.

7. Planting under the forest: Seedlings over one year old can be planted under the forest after being hardened.

The above-mentioned spore collection and preservation method is to collect dark brown leaves containing mature and uncracked golden dog spores, wash them with clean water to prevent mixing with other plant spores, place them in a dry place with newspaper as a pad, wait for the sporangium to completely crack, sieve them with a 200-mesh sieve to obtain pure spores, put them into a ziplock bag and store them in a refrigerator for later use.

The above-mentioned spore sowing method is to sow spores in early November every year. The substrate and spore suspension are prepared 24 hours in advance. The substrate is sterilized with a high-pressure sterilization crucible and then moistened and spread in a seedling box. The spores are made into a suspension and soaked for 24 hours. Spores can be sown after 24 hours. The spores are sown by spraying. Shake the spray pot each time to spray the spores evenly in the seedling box.

The matrix described above is prepared by mixing peat, coconut bran, fir sawdust, vermiculite and perlite in different proportions, among which the key point is the addition of fir sawdust, the specification of which is 0-6mm in diameter; the addition of fir sawdust makes the matrix have good air permeability and drainage, which can avoid the problem of plant root rot caused by excessive water boiling, and the high content of plant amino acids is conducive to the growth of spores, and the fir sawdust contains salicylic acid, which can disinfect and sterilize the root system of spores, promote the growth of spores, and improve the disease resistance of spores; and the use of fir sawdust does not require long-term fermentation and decomposition, so that the spores can enter the growth stage faster. At the same time, because the specification diameter of the fir sawdust is within a certain range and the particle size is small, it is particularly suitable for the cultivation of seedlings, so that the matrix is loose and not compacted; the fir sawdust can also have the effect of retaining fertilizer and moisture.

Furthermore, the matrix made by combining peat, coconut husk, vermiculite, perlite, etc. can provide comprehensive nutrition to the spores.

The above-mentioned seedling raising box is a container made of PP material with a sealed cover and is 22.3 cm long, 15.3 cm wide and 7.9 cm high.

The above-mentioned daily care method is to control the indoor temperature of the culture room at about 25°C, light 2000-3000Lx, and light cycle 12h/d. Keep the seedling box moist, spray Huaduoduo No. 1 water-soluble fertilizer every week after spore germination, and open the sealed cover of the seedling box for ventilation for 2h.

The above-mentioned method for sporophyte transformation is to add water to soak the prothallus after the spermatophore and archegonium are produced to accelerate fertilization and promote sporophyte transformation. The amount of water added is just enough to cover the prothallus, and the prothallus is placed on a shaker for shaking with an amplitude of 20 mm and a frequency of 200 rpm, and the reciprocating shaking is continued for 30 minutes.

The above-mentioned method of strengthening young sporophytes is to transplant them directly into non-woven container bags in greenhouses in February and March of the second year when they grow 4-5 leaves and the plant height is more than 2 cm. Cover them with a layer of drip-free mulch to keep warm and moisturize. At the same time, spray 500 times diluted carbendazim to prevent diseases. Remove the drip-free mulch after half a month.

Compared with the prior art, the present invention proposes the best spore sowing time and young sporophyte transplanting time, stipulates the specifications of the seedling container, and proposes measures to promote sporophyte transformation. It has the advantages of low cost, stable seedling emergence and short cycle. The prothallus begins to germinate in about 20 days, and the sporophyte begins to grow in about 75 days. The sporophyte transformation rate is more than 90%. In about 120 days, the sporophyte plant reaches a height of about 2 cm and can be transplanted.

Embodiment 2, please refer to Figures 2 to 3 of the specification, the present invention also proposes a device for breeding golden retrievers using spores.

In the prior art, the suitable temperature for breeding young spore seedlings of golden dog is 23-27°C. Therefore, when it exceeds 27°C, especially starting from 28°C, it will affect the growth of young spore seedlings of golden dog. The indoor temperature of the culture room is controlled at about 25°C, and 25°C is selected as the standard value.

Referring to the prior art, the atmospheric pressure at 25°C is 101.325 kPa (kilopascals); and when the temperature is between 21 and 50°C, the corrected value of the atmospheric pressure is 4.0 kPa. Therefore, at 28°C, the pressure inside the seedling box is greater than the pressure outside the seedling box by 12 kPa.

According to the conversion method: 1 kilogram of pressure is approximately equal to 0.1Mpa (megapascals), one kilogram of force is equal to 9.8N (Newtons), 1Mpa=1000kpa; it can be seen that: 12kPa is equivalent to 1.176N of force.

Taking into account that during daily care, it is only opened when water-soluble fertilizer is sprayed weekly after spore germination, and in the remaining time, since the seedling box is sealed, the inside of the seedling box is prone to excessively high temperature, which will affect the growth of young spore seedlings of golden dog; therefore, in the present invention, a vent 2 is provided on the sealing cover of the seedling box, the vent 2 is a rectangular groove structure, and a sealing plate 3 with a rectangular plate structure is movably provided in the vent 2, the sealing plate 3 is a transparent plastic plate, which does not affect the daily light care and care of young spore seedlings of golden dog, one end of the sealing plate 3 is fixedly provided with a rotating shaft 4, the rotating shaft 4 is rotatably connected in the vent 2, the surrounding of the sealing plate 3 is sealed with a sealing ring 6, one side of the lower surface of the sealing plate 3 is fixed by a tension spring 5, the tension spring 5 is fixed on the inner wall of the vent 2, and the other side is blocked by a limit block 7 to prevent the sealing plate 3 from rotating downward; the vent 2 is communicated with the inside of the seedling box.

The sum of the tension of the tension spring 5 and the gravity of the sealing plate 3 is 1.176 Newtons; that is, when the temperature inside the seedling box exceeds 28°C, the pressure generated inside the seedling box can just push the sealing plate 3 upward to open, thereby achieving pressure relief and heat dissipation, ensuring that the temperature inside the seedling box does not exceed 28°C, achieving the purpose of automatic management and maintenance, without the need for frequent manual care, and with a simple structure, without the need to use electronic equipment such as temperature sensors, with low cost, and promoting the growth of young spore seedlings of Golden Dog.

It should be noted that the sealing cover 1 provided in the present invention can also be used to cultivate other seedlings. According to the growth conditions of other seedlings, a tension spring 5 with appropriate tension and a sealing plate 3 with appropriate gravity can be used. It can be adjusted adaptively and has a wide range of applications.

It is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modification, equivalent substitution or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (10)

1. A method for breeding a golden hair dog by using spores, which is characterized by comprising the following steps:

s1, spore collection and preservation: collecting mature spores of the golden hair dogs, and storing in a refrigerator refrigerating chamber for standby;

s2, sowing spores: sterilizing the substrate, adding water, wetting and spreading in a seedling box, preparing spores into suspension, and spraying on the surface of the substrate;

s3, daily management and protection: after sowing spores, placing the seedling box in a culture room, waiting for germination of the protoleaf body, and performing daily management and protection until the sporophyte is transplanted;

S4, sporophyte transformation: after the protophylls are mature, a sperm device and a neck ovum device are generated, water is added for soaking, and the mixture is put into a shaking table for shaking, so that the conversion of the sporophytes is promoted, and the sporophytes are waited to grow into juvenile sporophytes;

S5, strengthening seedlings of juvenile sporophytes: when the young sporophytes grow to 4-5 leaves and the plant height is more than 2cm, transplanting the young sporophytes into a greenhouse for strong seedling culture;

S6, seedling training: seedling training is carried out half month before planting under the forest;

S7, planting under the forest: the seedlings above one year are planted under the forest after the seedling is trained.

2. A method for breeding a golden hair dog using spores according to claim 1, wherein: the method for collecting and storing spores in the S1 comprises the following steps: collecting dark brown mature uncracked leaves of Cibotium barometz spores, cleaning with clear water to prevent mixing with other plant spores, placing in a dry place with newspaper pad, sieving with 200 mesh sieve to obtain pure spores, packaging into self-sealing bags, and storing in refrigerator refrigerating chamber.

3. A method for breeding a golden hair dog using spores according to claim 1, wherein: the method for sowing spores in S2 is to prepare a matrix and spore suspension liquid at the beginning of 11 months each year, prepare the matrix and spore suspension liquid 24 hours in advance, sterilize the matrix by using an autoclave pot, moisten and spread the matrix in a seedling box, soak the spore suspension liquid for 24 hours, sow spores after 24 hours, and adopt spraying and sowing, and shake a watering can to uniformly spray the spores in the seedling box when spraying and sowing each time.

4. A method for breeding a golden hair dog using spores according to claim 1, wherein: the matrix adopts peat, coconut chaff, fir sawdust, vermiculite and perlite, and the specification of the fir sawdust is 0-6mm in diameter.

5. A method for breeding a golden hair dog using spores according to claim 1, wherein: the seedling raising box is a container made of PP material with the length of 22.3cm, the width of 15.3cm and the height of 7.9cm and provided with a sealing cover.

6. A method for breeding a golden hair dog using spores according to claim 1, wherein: the daily management and protection method in the step S3 is that the indoor temperature of a culture room is controlled to be about 25 ℃, the illumination is 2000-3000Lx, the illumination period is 12h/d, the interior of a seedling box is kept moist, more No. 1 water-soluble fertilizer is sprayed every week after spores germinate, and a sealing cover of the seedling box is opened every day for ventilation for 2h.

7. A method for breeding a golden hair dog using spores according to claim 1, wherein: the sporophyte conversion method in the step S4 is that after the production of a protophyll sperm device and a cervical ovum device, water is added for soaking, fertilization is quickened, sporophyte conversion is promoted, the water addition quantity is that the protophyll is just over, the protophyll is placed on a shaking table for shaking, the shaking amplitude is 20mm, the shaking frequency is 200rpm, and the shaking is continued for 30min in a reciprocating mode.

8. A method for breeding a golden hair dog using spores according to claim 1, wherein: the seedling strengthening method of the young sporophytes in the S5 is that when the young sporophytes grow to 4-5 leaves in 2 and 3 months of the second year, the young sporophytes are transplanted into a greenhouse non-woven fabric container bag with the plant height of more than 2cm, a layer of non-dripping mulching film is covered for heat preservation and moisture preservation, meanwhile, 500 times of liquid of carbendazim is sprayed for disease prevention, and the non-dripping mulching film is uncovered after half a month.

9. A device for breeding a golden hair dog by utilizing spores, which is characterized in that: the method for breeding the young golden hair dog spores according to any one of claims 1-8, comprising a sealing cover (1) arranged on a seedling raising box, wherein a vent (2) is arranged on the sealing cover (12), a sealing plate (3) is movably arranged in the vent (2), one end of the sealing plate (3) is fixedly provided with a rotating shaft (4), the rotating shaft (4) is rotationally connected in the vent (2), one side of the lower end of the sealing plate (3) is movably attached with a limiting block (7), the other side of the lower end of the sealing plate is fixedly connected with a tension spring (5), the lower end of the tension spring (5) is fixed on the inner wall of the vent (2), and the limiting block (7) is fixedly connected on the inner wall of the vent (2).

10. A method for breeding a golden hair dog using spores according to claim 9, wherein: the sum of the tension spring (5) and the gravity of the sealing plate (3) is equal to the difference between the pressure at the highest threshold temperature inside the seedling raising box and the pressure at the highest threshold temperature outside the seedling raising box.