CN1186596A - Storing liquid for blood - Google Patents
Storing liquid for blood Download PDFInfo
- Publication number
- CN1186596A CN1186596A CN96119715A CN96119715A CN1186596A CN 1186596 A CN1186596 A CN 1186596A CN 96119715 A CN96119715 A CN 96119715A CN 96119715 A CN96119715 A CN 96119715A CN 1186596 A CN1186596 A CN 1186596A
- Authority
- CN
- China
- Prior art keywords
- blood
- glucose
- liquid
- mannitol
- storing liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The storing liquid is compounded with sodium citrate, citric acid, NaN2PO4, Na2HPO4, mannitol, ATP, glucose and adenine in certain proportion as well as distilled water. Adding energy-supplying ATP into traditional storing liquid with ACD, CPD and PIGPA can raise the energy collecting and nutrient synthesizing capacity of cells; the miniature amount of mannitol has the function of protecting cell membrane; and the inorganic salt NaH2PO4 and Na2HPO4 as buffering agent can regulate pH value of storing liquid. The present invention has a blood storing period of 35 days and the stored blood may be high-pressure and cobalt 60 disinfected.
Description
The invention belongs to anticoagulant for storage of whole blood, a kind of specifically anticoagulant for storage of whole blood of the usefulness of transfusing blood.
Continue to use ACD (acid-cltrate-dertrose) in the world as the existing 40 years history of anticoagulant for storage of whole blood, ACD also has different methods of completing the square, and commonly used is ACDI number.Wherein the acid of structure rafter is 4.7g/L, and citric acid trisodium 13.3g/L, glucose are 30g/L.The citric acid trisodium not only can anti-freezing, and can not permeate through cell membranes, non-confrontational intracellular osmotic pressure, thereby stop the dissolving of cell, glucose is the essential nutrition of erythrocyte metabolism, can prolong the erythrocytic holding time.Prevent haemolysis, glucose can make in the cell organic phosphor effect slow again, prevents hematoclasis.The mixed liquor of citric acid trisodium and glucose, during by autoclaving, glucose meeting coking, solution is sepia, and can cause transfusion reaction, makes slightly acidifying of solution as adding citric acid, can prevent the glucose coking, and the ATP in the storage blood is had stabilization.
As make slightly acidifying of solution with simple citric acid.Can prevent the glucose coking, and the ATP in the storage blood is had stabilization.
As preserving blood with simple sodium citrate, be no more than 5 days in effective storage life below 4 ℃, usefulness sodium citrate-Glucose Liquid can be preserved 5-14 days, and then in the time of 21 days, red blood cell survival rate in vivo is 70% to usefulness ACD liquid preservation liquid.
Gibson (nineteen fifty-seven) thinks that ACD Precerving liquid PH is low excessively, blood is in contact with it and still has easy damage and destroy erythrocytic effect, as cell expansion, cause that mistake potassium and organic phosphor decompose rapidly, so be set forth in the ACD preservation liquid and add phosphate, to improve the PH that preserves liquid, promptly form so-called CPD and preserve liquid (Cirtate-phosphate deriose).Erythrocytic storage life is prolonged, find that simultaneously ACD blood is in the preservation process, the erythrocytic oxygen function of putting descends rapidly, and CPD blood to put oxygen function ratio ACD blood strong, event CPD preserves liquid will the trend that replaces ACD preservation liquid, and now the PH with ACD and CPD preservation liquid preservation blood is compared to table 2-8-1
Table 2-8-1 ACD and CPD preserve liquid PH relatively
| Temperature | Condition | ??ACD | ???CPD |
| ???24℃ | The simple liquid of preserving | ???5.0 | ????5.6 |
| ???24℃ | Preserve liquid+blood | ???6.9 | ????7.1 |
| ????4℃ | Preserve liquid+blood | ???7.4 | ????7.5 |
ATP is erythrocytic energy source.Can represent erythrocytic activity in the body, the fat purine is the part of ATP molecule.As in ACD or CPB preservation liquid, adding adenine, can make storage life extend to 35 days, so the someone advocates that the prescription of preservation effect the best is phosphate, inosine, glucose, pyruvic acid and adenine in recent years, promptly PIGPA preserves liquid (Phosphate-lnosine-gluccse-pyruvlc acld-adealne).Above select from " clinical examination basis " 1993 People's Health Publishers (P112-113 page or leaf) of Li Disheng chief editor.
By long experiment, we find among above-mentioned traditional blood Precerving liquid ACD, CPD, the PIGPA glucose, adenine are only arranged, after cell exsomatizes, because of its adopt can ability and ability of synthetic nutrient lower, this is not enough to the cell energy supply after exsomatizing, and after red blood cell exsomatizes, cell membrane is yielding, so red blood cell cracky, haemolysis, thereby lowered the survival rate of red blood cell after again in the human body, the erythrocytic motility rate again of above-mentioned traditional Precerving liquid only is 70%, and it is right not have buffering in CPD, PIGPA, and it only is NaH that institute adds phosphate
2PO
4, can't align the pH value of preserving liquid, and with human body in the buffer system of blood plasma misfit.
Domestic main anticoagulant for storage of whole blood commonly used is that ACD, CPD, PIGPA are owing to the reason of material and cost is not popularized at home at present.
At the present situation of above-mentioned present anticoagulant for storage of whole blood, purpose of the present invention aims to provide a kind of cell that can improve and adopts energy ability and synthetic nutrient ability, improves erythrocytic motility rate again, makes the moderate blood preservative fluid of pH value.
The objective of the invention is to realize in the following manner: a kind of anticoagulant for storage of whole blood, comprise sodium citrate, citric acid, glucose, adenine, the formulation ratio of this preservation liquid is:
Sodium citrate 2.35-2.65g mannitol 0.02-0.08g
Citric acid 0.25-0.40g adenosine triphosphate 0.02-0.08g
NaH
2PO
40.15-0.28g glucose 2.0-3.0g
Na
2HPO
43.0-4.5g adenine 0.005-0.015g
The above-mentioned raw materials adding distil water is got final product to 100ml.
Described sodium citrate, citric acid, NaH
2PO
4, Na
2HPO
4, mannitol, Adenosine triphosphate intoxicated (ATP), glucose, adenine is injection.
The every 15-25ml of above-mentioned preservation liquid can preserve 100ml blood.
The present invention has added ATP, mannitol, NaH in anticoagulants for storage of whole blood such as traditional ACD, CPD
2PO
4/ Na
2HPO
4Slow right.
Micro-injection mannitol among the present invention; cell membrane has protective effect; and do not influence function of plasma membrane; thereby improved erythrocytic motility rate again; reach about 80%, diuresis mannitol 20g-100g/100ml, the mannitol among the present invention then only are 0.02-0.08g/100ml; so can ignore to the diuresis of human body, and the mineral salt buffering is to NaH
2PO
4/ Na
2HPO
4Then can align the pH value in the Precerving liquid, make it to reach the pH value of human plasma, be that the main mineral salt buffering in the human plasma is right, prolongs erythrocytic storage life.
The present invention can use sterilizations such as high pressure, cobalt, and pH value sees Table 1.
Table 1
The blood of preserving is cerise (and the blood of ACD, CPD, PIGPA preservation is kermesinus), and storage life can reach 35 days.Composition in this preservation liquid meets the nineteen ninety-five version, and " the Pharmacopoeia of People's Republic of China requirement does not have any damage and pays effect human body.
| Temperature | Condition | PH value |
| ?24℃ | The simple liquid of preserving | ????5.0-5.6 |
| ?24℃ | Preserve liquid+blood | ????7.0 |
| ?4℃ | Preserve liquid+blood | ???7.35-7.45 |
Embodiment 1: sodium citrate 2.65g mannitol 0.02g citric acid 0.25g adenosine triphosphate 0.2gNaH
2PO
40.15g glucose 2.0gNa
2HPO
43.0g adenine 0.005g adding distil water gets final product to 100ml.Above-mentioned each raw material is injection.Embodiment 2 sodium citrate 2.50g mannitol 0.04g citric acid 0.25g adenosine triphosphate 0.02gNaH
2PO
46.20g glucose 0.55gNa
2HPO
43.0g adenine 0.009g adding distil water gets final product to 100ml.Above-mentioned each raw material is injection.
Claims (1)
1, a kind of anticoagulant for storage of whole blood comprises sodium citrate, citric acid, glucose, adenine, it is characterized in that the formulation ratio of this preservation liquid is:
Sodium citrate 2.35-2.65g mannitol 0.02-0.08g
Citric acid 0.25-0.40g adenosine triphosphate 0.02-0.08g
NaH
2PO
40.15-0.28g glucose 2.0-3.0g
Na
2HPO
43.0-4.5g adenine 0.005-0.015g
The above-mentioned raw materials adding distil water is got final product to 100ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96119715A CN1186596A (en) | 1996-12-31 | 1996-12-31 | Storing liquid for blood |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96119715A CN1186596A (en) | 1996-12-31 | 1996-12-31 | Storing liquid for blood |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1186596A true CN1186596A (en) | 1998-07-08 |
Family
ID=5125948
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN96119715A Pending CN1186596A (en) | 1996-12-31 | 1996-12-31 | Storing liquid for blood |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1186596A (en) |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008132101A3 (en) * | 2007-04-30 | 2009-01-15 | Unilever Plc | Method of treating hair |
| CN101904331A (en) * | 2010-07-14 | 2010-12-08 | 樊福好 | Blood sample protecting solution and method for collecting blood sample |
| CN102422835A (en) * | 2011-10-20 | 2012-04-25 | 中国人民解放军军事医学科学院附属医院 | Blood maintenance liquid, preparation method thereof and application thereof |
| CN103250694A (en) * | 2005-02-17 | 2013-08-21 | 辛辛那提大学 | Composition and method for storing erythrocyte |
| US9314014B2 (en) | 2004-02-18 | 2016-04-19 | University Of Maryland, Baltimore | Compositions and methods for the storage of red blood cells |
| CN105651568A (en) * | 2015-12-28 | 2016-06-08 | 西安交通大学第附属医院 | Preparation method of control material for blood cell analyzer |
| CN105850977A (en) * | 2015-01-23 | 2016-08-17 | 北京永泰生物制品有限公司 | Blood preservation solution |
| CN106614524A (en) * | 2016-11-26 | 2017-05-10 | 中国人民解放军第四军医大学 | Preserving fluid for mesenchymal stem cells and preserving method thereof |
| CN107083382A (en) * | 2017-05-25 | 2017-08-22 | 广州奇辉生物科技有限公司 | A kind of blood preseration agent and its application for being used to protect dissociative DNA |
| CN109644994A (en) * | 2019-01-31 | 2019-04-19 | 华东师范大学 | A kind of animal erythrocyte saves the preparation method and its application of liquid |
| CN109892320A (en) * | 2019-03-20 | 2019-06-18 | 浙江省人民医院 | A kind of cell-preservation liquid and its preparation method and application |
| CN111713487A (en) * | 2020-06-30 | 2020-09-29 | 上海市血液中心 | Reagent erythrocyte preservation system and preparation method thereof |
| CN114732008A (en) * | 2022-06-13 | 2022-07-12 | 深圳瑞亚力集团有限公司 | Erythrocyte preservation solution, preparation method thereof and erythrocyte suspension |
| CN116941603A (en) * | 2023-07-21 | 2023-10-27 | 浙江康嘉基因技术有限公司 | Preparation method and application of novel hydroformylation chicken erythrocyte |
-
1996
- 1996-12-31 CN CN96119715A patent/CN1186596A/en active Pending
Cited By (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9314014B2 (en) | 2004-02-18 | 2016-04-19 | University Of Maryland, Baltimore | Compositions and methods for the storage of red blood cells |
| CN103250694A (en) * | 2005-02-17 | 2013-08-21 | 辛辛那提大学 | Composition and method for storing erythrocyte |
| US8182798B2 (en) | 2007-04-30 | 2012-05-22 | Conopco, Inc. | Method of treating hair |
| EA016987B1 (en) * | 2007-04-30 | 2012-08-30 | Унилевер Н.В. | Method of treating hair |
| WO2008132101A3 (en) * | 2007-04-30 | 2009-01-15 | Unilever Plc | Method of treating hair |
| CN101904331A (en) * | 2010-07-14 | 2010-12-08 | 樊福好 | Blood sample protecting solution and method for collecting blood sample |
| CN101904331B (en) * | 2010-07-14 | 2014-04-16 | 樊福好 | Blood sample protecting solution and method for collecting blood sample |
| CN102422835A (en) * | 2011-10-20 | 2012-04-25 | 中国人民解放军军事医学科学院附属医院 | Blood maintenance liquid, preparation method thereof and application thereof |
| CN105850977B (en) * | 2015-01-23 | 2018-10-26 | 北京永泰生物制品有限公司 | Anticoagulant for storage of whole blood |
| CN105850977A (en) * | 2015-01-23 | 2016-08-17 | 北京永泰生物制品有限公司 | Blood preservation solution |
| CN105651568A (en) * | 2015-12-28 | 2016-06-08 | 西安交通大学第附属医院 | Preparation method of control material for blood cell analyzer |
| CN106614524A (en) * | 2016-11-26 | 2017-05-10 | 中国人民解放军第四军医大学 | Preserving fluid for mesenchymal stem cells and preserving method thereof |
| CN106614524B (en) * | 2016-11-26 | 2020-10-09 | 中国人民解放军第四军医大学 | Preservation solution and preservation method for mesenchymal stem cells |
| CN107083382B (en) * | 2017-05-25 | 2018-07-31 | 广州奇辉生物科技有限公司 | A kind of blood preseration agent and its application for protecting dissociative DNA |
| CN107083382A (en) * | 2017-05-25 | 2017-08-22 | 广州奇辉生物科技有限公司 | A kind of blood preseration agent and its application for being used to protect dissociative DNA |
| CN109644994A (en) * | 2019-01-31 | 2019-04-19 | 华东师范大学 | A kind of animal erythrocyte saves the preparation method and its application of liquid |
| CN109892320A (en) * | 2019-03-20 | 2019-06-18 | 浙江省人民医院 | A kind of cell-preservation liquid and its preparation method and application |
| CN111713487A (en) * | 2020-06-30 | 2020-09-29 | 上海市血液中心 | Reagent erythrocyte preservation system and preparation method thereof |
| CN114732008A (en) * | 2022-06-13 | 2022-07-12 | 深圳瑞亚力集团有限公司 | Erythrocyte preservation solution, preparation method thereof and erythrocyte suspension |
| CN116941603A (en) * | 2023-07-21 | 2023-10-27 | 浙江康嘉基因技术有限公司 | Preparation method and application of novel hydroformylation chicken erythrocyte |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US9314014B2 (en) | Compositions and methods for the storage of red blood cells | |
| CN1186596A (en) | Storing liquid for blood | |
| CA2598366C (en) | Compositions and methods for the storage of red blood cells | |
| KR950015064B1 (en) | Solution for the preservation of organs | |
| US20230338235A1 (en) | Blood storage container containing aqueous composition for the storage of red blood cells | |
| JP3253131B2 (en) | Transplant organ solution | |
| EP0510185B1 (en) | Blood platelet storage medium | |
| EP0142002B1 (en) | Heat sterilizable storage solution for red blood cells and blood bags containing it | |
| US5945272A (en) | Plasma expanders and blood substitutes | |
| JPS6360931A (en) | Solution for preserving blood or blood preparation and preservation of blood or blood preparation using said solution | |
| CN102524240A (en) | Efficient and environment-friendly remains preservative and application thereof | |
| EA003171B1 (en) | Methods and compositions for use in perfusion applications | |
| CA1246449A (en) | Drug kit or drug composition for ischaemic damage | |
| KR102570322B1 (en) | Solutions for increasing the stability and shelf life of an organ and tissue preservation solution | |
| Herrero et al. | Evaluation of a preservation solution containing fructose-1, 6-diphosphate and mannitol using the isolated perfused rat kidney. Comparison with Euro-Collins and University of Wisconsin solutions | |
| CN1273016C (en) | Kidney preservative fluid | |
| US3703438A (en) | Method for the stabilization of blood | |
| JPH0822801B2 (en) | Solution for organ preservation | |
| CN108835243A (en) | It is a kind of for making the preservation method of the mushroom of mushroom sauce | |
| Mazor et al. | Prolonged storage of red cells with ammonium chloride and mannitol | |
| CN1044575A (en) | Preserve the method for bone, cartilage, skin with honey | |
| CN104069127A (en) | Composition containing electrolyte balance salt and bromogeramine and preparation method thereof | |
| CN116267889A (en) | Preservation solution for prolonging preservation time of whole bone marrow and use method | |
| CN116458495A (en) | Cell freezing solution for frozen storage of organoids and preparation method and application thereof | |
| Shibamoto et al. | Fructose 1, 6-diphosphate augments paraquat injury in isolated dog lungs |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C01 | Deemed withdrawal of patent application (patent law 1993) | ||
| WD01 | Invention patent application deemed withdrawn after publication |