CN118496987B - Equipment and process for separating and purifying collagen tripeptide by adopting enzymolysis method - Google Patents
Equipment and process for separating and purifying collagen tripeptide by adopting enzymolysis method Download PDFInfo
- Publication number
- CN118496987B CN118496987B CN202410961366.9A CN202410961366A CN118496987B CN 118496987 B CN118496987 B CN 118496987B CN 202410961366 A CN202410961366 A CN 202410961366A CN 118496987 B CN118496987 B CN 118496987B
- Authority
- CN
- China
- Prior art keywords
- separation
- filter
- purification
- tube
- pipe
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000000034 method Methods 0.000 title claims abstract description 37
- 102000008186 Collagen Human genes 0.000 title claims abstract description 30
- 108010035532 Collagen Proteins 0.000 title claims abstract description 30
- 229920001436 collagen Polymers 0.000 title claims abstract description 30
- 230000008569 process Effects 0.000 title claims abstract description 16
- 238000000926 separation method Methods 0.000 claims abstract description 53
- 238000000746 purification Methods 0.000 claims abstract description 51
- 230000007246 mechanism Effects 0.000 claims abstract description 43
- 238000001914 filtration Methods 0.000 claims abstract description 41
- 239000007788 liquid Substances 0.000 claims abstract description 28
- 239000000706 filtrate Substances 0.000 claims abstract description 24
- 239000000463 material Substances 0.000 claims abstract description 14
- 230000002093 peripheral effect Effects 0.000 claims description 21
- 238000006243 chemical reaction Methods 0.000 claims description 18
- 239000002994 raw material Substances 0.000 claims description 14
- 238000003756 stirring Methods 0.000 claims description 11
- 108091005804 Peptidases Proteins 0.000 claims description 8
- 239000004365 Protease Substances 0.000 claims description 8
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 8
- 239000011148 porous material Substances 0.000 claims description 7
- 238000007599 discharging Methods 0.000 claims description 6
- 241000251468 Actinopterygii Species 0.000 claims description 5
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims description 5
- 235000017491 Bambusa tulda Nutrition 0.000 claims description 5
- 241001330002 Bambuseae Species 0.000 claims description 5
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims description 5
- 239000011425 bamboo Substances 0.000 claims description 5
- 210000000988 bone and bone Anatomy 0.000 claims description 4
- 244000144972 livestock Species 0.000 claims description 3
- 244000144977 poultry Species 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 16
- 238000003825 pressing Methods 0.000 abstract description 6
- 238000011010 flushing procedure Methods 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 description 5
- 238000010586 diagram Methods 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 230000006835 compression Effects 0.000 description 2
- 238000007906 compression Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000002893 slag Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000021164 cell adhesion Effects 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000000622 irritating effect Effects 0.000 description 1
- 238000010030 laminating Methods 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 231100000344 non-irritating Toxicity 0.000 description 1
- 235000020939 nutritional additive Nutrition 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D29/00—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor
- B01D29/50—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor with multiple filtering elements, characterised by their mutual disposition
- B01D29/56—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor with multiple filtering elements, characterised by their mutual disposition in series connection
- B01D29/58—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor with multiple filtering elements, characterised by their mutual disposition in series connection arranged concentrically or coaxially
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D29/00—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor
- B01D29/62—Regenerating the filter material in the filter
- B01D29/66—Regenerating the filter material in the filter by flushing, e.g. counter-current air-bumps
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D29/00—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor
- B01D29/62—Regenerating the filter material in the filter
- B01D29/66—Regenerating the filter material in the filter by flushing, e.g. counter-current air-bumps
- B01D29/68—Regenerating the filter material in the filter by flushing, e.g. counter-current air-bumps with backwash arms, shoes or nozzles
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
- C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
- C07K5/08—Tripeptides
- C07K5/0802—Tripeptides with the first amino acid being neutral
- C07K5/0804—Tripeptides with the first amino acid being neutral and aliphatic
- C07K5/0806—Tripeptides with the first amino acid being neutral and aliphatic the side chain containing 0 or 1 carbon atoms, i.e. Gly, Ala
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/12—Purification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Sustainable Development (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Gastroenterology & Hepatology (AREA)
- General Chemical & Material Sciences (AREA)
- Toxicology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention discloses equipment and a process for separating and purifying collagen tripeptide by adopting an enzymolysis method, which relate to the technical field of separation and purification and comprise a separation and purification box, a filtrate drainage mechanism and a circulating drainage mechanism, wherein a cylinder frame is arranged at the upper end of the separation and purification box, a rotating shaft pipe extending into the separation and purification box is rotatably arranged on the cylinder frame, a material guide hole communicated with the inside of the separation and purification box is arranged on the pipe wall of the rotating shaft pipe, a feeding box rotatably connected with the rotating shaft pipe is also arranged on the cylinder frame, a rotation control mechanism for controlling the rotation of the rotating shaft pipe is also arranged on the cylinder frame, a plurality of filtering surrounding cylinders with different radiuses are concentrically arranged in the separation and purification box, and when a pinch roller is pressed down to an inclined surface block, the inclined surface block drives the arc-shaped block to exert a pressing effect on partial feed liquid below the arc-shaped block, so that the effect of flushing the filtering hole is realized, the smoothness of the filtering hole is kept, and the separation and purification precision is improved.
Description
Technical Field
The invention relates to the technical field of separation and purification, in particular to equipment and a process for separating and purifying collagen tripeptide by adopting an enzymolysis method.
Background
Collagen tripeptides are widely used in nutritional additives, cosmetics and various functional foods for their various benefits, including promotion of skin and bone repair, enhancement of mineral absorption, antioxidation, promotion of collagen synthesis, and the like. In the process of preparing collagen tripeptide, animal tissues such as fish, pigskin and the like are main raw material sources. Among these sources, fish skin has become the material of choice for the preparation of collagen tripeptides due to its high safety, good cell adhesion, transparency and non-irritating properties.
However, in the preparation process of collagen tripeptide, in the process of separating and purifying collagen tripeptide by adopting an enzymolysis method, the collagen tripeptide needs to be subjected to alkaline environment, so that the hydrolysis is incomplete, and the enzymolysis liquid has partial macromolecular substances, so that filter holes are easy to block, the fluidity of the feed liquid is influenced, and the reaction effect of protease is influenced. After each separation, the inside of the apparatus must be cleaned in order to maintain the performance and separation effect of the apparatus. This process is time consuming and affects the continuity of production, thereby reducing the overall production efficiency.
Therefore, it is necessary to provide an apparatus and a process for separating and purifying collagen tripeptide by using an enzymolysis method, so as to solve the problems in the prior art.
Disclosure of Invention
In order to achieve the above purpose, the present invention provides the following technical solutions: the utility model provides an adopt equipment of enzymolysis method separation purification collagen tripeptide, includes separation and purification case, filtrate drainage mechanism and circulation drainage mechanism, the section of thick bamboo frame is installed to separation and purification case upper end, rotate on the section of thick bamboo frame and install and stretch into the inside pivot pipe of separation and purification case, be equipped with on the pivot pipe wall with the inside guide hole that communicates of separation and purification case, still install on the section of thick bamboo frame with the feeding case that the pivot pipe rotates to be connected, still install on the section of thick bamboo frame and be used for controlling pivot pipe pivoted rotary control mechanism, the inside concentric filtration enclosure that installs a plurality of different radiuses of separation and purification case, filtrate drainage mechanism is used for guiding the filtrate to pass through the filtration enclosure, be equipped with the filtration pore on the filtration enclosure, just filtrate drainage mechanism can guide the filter residue on the filtration enclosure filtration pore and break away from the filtration pore, circulation drainage mechanism is used for the drainage to be close to in the feed liquid entering the feeding case of the filtration enclosure of pivot pipe one side, be equipped with the puddler that is used for the stirring on the pivot pipe.
Further, as a preferable option, the filter enclosures are arranged sequentially from inside to outside, and the pore diameters of the filter holes thereof are sequentially reduced.
Further, as the preference, be formed with the circular chamber that encloses of a plurality of different radiuses between a plurality of filtration of different radiuses, filtrate drainage mechanism is including setting up solid fixed ring and a plurality of dull and stereotyped seat in separation and purification case top respectively, gu fixed ring is fixed to be cup jointed on the pivot pipe, installs radial branch on the solid fixed ring, the pinch roller is installed in the rotation of branch outer end, dull and stereotyped seat is circumference range and is located pinch roller circumference rotation trajectory's below, every the inclined plane piece that is used for with the pinch roller contact is all installed to the upper end of dull and stereotyped seat, every the pole one that corresponds with enclosing the circular chamber is all installed to the lower extreme of dull and stereotyped seat, the arc chock that corresponds with enclosing the circular chamber is installed to the lower extreme of pole, the arc chock passes through spring one and is connected with the upper end of separation and purification case, and enclose the mutual laminating of the side of arc chock on the circular chamber, and the filter hole on the filtration encloses the barrel is located the below of arc chock, the inclined plane piece down displacement can be rolled in proper order to the in the process of the rotation of pinch roller circumference.
Further, as an preference, a peripheral cavity is formed between the filtering surrounding cylinder and the separation and purification box, which is close to the outer side of the separation and purification box, an annular plug block is installed at the top of the peripheral cavity, a column groove is formed in the upper end of the annular plug block, a second pole is connected to the column groove in a sliding manner, the upper end of the second pole is connected to the separation and purification box, and the annular plug block is connected with the upper end of the separation and purification box through a second spring.
Further, preferably, a discharging pipe communicated with the peripheral cavity is arranged on the separation and purification box.
Further, as the preference, the barrel cavity of the filtration enclosure near one side of the rotating shaft pipe is set as an enzymolysis reaction cavity, the circulation drainage mechanism comprises a discharge pipe communicated with the enzymolysis reaction cavity, the discharge pipe comprises a first column cavity, a second column cavity and a third column cavity, the first column cavity, the second column cavity and the third column cavity are respectively arranged at the upper part, the third column cavity is connected with a feeding box through a circulation pipe, a control pump is arranged on the circulation pipe, a first screw blade rod is arranged in the first column cavity, the upper end of the first screw blade rod is connected with the lower end of the rotating shaft pipe, a second column sleeve connected with the lower end of the screw blade rod is arranged in the second column cavity, the screw blade direction of the first screw blade rod is opposite to the screw blade direction of the second screw blade rod, and the radius of the second column cavity is larger than that of the first column cavity.
Further, preferably, the distance between the outer ring edge of the first screw blade rod and the cavity wall of the first column cavity is equal to one half of the radius of the first column cavity.
Further, preferably, the outer ring edge of the second screw blade rod is attached to the wall of the third column cavity.
Further, preferably, the rotation control mechanism comprises a motor installed on the cylinder frame, a gear is installed at the output end of the motor, and the gear is in meshed connection with a toothed ring sleeved on the rotating shaft tube.
A process for separating and purifying collagen tripeptide by adopting an enzymolysis method comprises the following steps:
step one: treating livestock and poultry skin bones or fish skin scales as raw materials, preparing protease for enzymolysis reaction, and respectively introducing the protease into a feeding box;
Step two: starting a rotation control mechanism to drive a rotary shaft tube to rotate and stir feed liquid, and simultaneously, driving a filtrate drainage mechanism to guide filtrate to separate from a filter hole through a filter surrounding tube and filter residues on a filter hole of the filter surrounding tube;
Step three: the material liquid in the filtering surrounding cylinder close to one side of the rotary shaft pipe is drained through the circulating drainage mechanism to enter the feeding box, so that circulating flow of the material liquid is formed.
Compared with the prior art, the invention provides equipment and a process for separating and purifying collagen tripeptide by adopting an enzymolysis method, which have the following beneficial effects:
According to the invention, the stirring rod is driven to rotate through the rotating shaft pipe, raw materials pass through the material guide hole and enter the enzymolysis reaction cavity under the action of centrifugal force to perform enzymolysis reaction, so that the raw materials can be better dispersed and uniformly mixed, the enzymolysis reaction effect and reaction rate are improved, filter residues in the raw materials are filtered through the arranged filtering surrounding cylinder, the generated collagen tripeptide raw materials can pass through the filtering surrounding cylinder, and the filtrate drainage mechanism driven by the rotating shaft pipe is arranged, so that the raw materials can be treated to a certain extent, namely, when the pressing wheel presses the inclined surface block downwards, the inclined surface block drives the arc-shaped block to perform compression effect on partial raw materials below the arc-shaped block, and the inclined surface block drives the arc-shaped block to perform suction effect on the partial raw materials below the arc-shaped block through the first driving spring, so that the inclined surface block has the effect of flushing the filtering holes, the smoothness of the filtering holes is maintained, and the separation and purification precision is improved.
Drawings
FIG. 1 is a schematic diagram of an apparatus for separating and purifying collagen tripeptide by using an enzymolysis method;
FIG. 2 is an internal cross-sectional view of an apparatus for separating and purifying collagen tripeptides by an enzymatic hydrolysis method;
FIG. 3 is a schematic diagram of the structure of a rotating shaft tube of an apparatus for separating and purifying collagen tripeptide by using an enzymolysis method;
FIG. 4 is a schematic diagram showing a partial structure of a filtrate drainage mechanism in an apparatus for separating and purifying collagen tripeptide by an enzymolysis method;
In the figure: 1. separating and purifying the box; 2. a cartridge holder; 3. a rotary shaft tube; 4. a rotation control mechanism; 5. a filtering enclosure; 6. a filtrate drainage mechanism; 7. a circulation drainage mechanism; 11. a discharge pipe; 21. a feed box; 31. a material guiding hole; 32. a stirring rod; 41. a motor; 42. a gear; 43. a toothed ring; 61. a fixing ring; 62. a support rod; 63. a pinch roller; 64. a flat plate seat; 65. a first pole; 66. arc-shaped chocks; 67. a first spring; 68. a bevel block; 69. an annular chock; 610. a second pole; 611. a second spring; 691. a column groove; 71. a discharge pipe; 72. column cavity I; 73. a second column cavity; 74. column cavity III; 75. a first screw blade rod; 76. a column sleeve; 77. a second spiral blade rod; 78. a circulation pipe; 79. the pump is controlled.
Detailed Description
Referring to fig. 1-4, the present invention provides a technical solution: the device for separating and purifying collagen tripeptide by adopting an enzymolysis method comprises a separation and purification box 1, a filtrate drainage mechanism 6 and a circulating drainage mechanism 7, wherein a barrel frame 2 is arranged at the upper end of the separation and purification box 1, a rotating shaft pipe 3 extending into the separation and purification box 1 is rotatably arranged on the barrel frame 2, a material guide hole 31 communicated with the inside of the separation and purification box 1 is arranged on the pipe wall of the rotating shaft pipe 3, a feeding box 21 rotatably connected with the rotating shaft pipe 3 is also arranged on the barrel frame 2, a rotary control mechanism 4 for controlling the rotation of the rotating shaft pipe 3 is also arranged on the barrel frame 2, a plurality of filtering surrounding barrels 5 with different radiuses are concentrically arranged in the separation and purification box 1, the filtrate drainage mechanism 6 is used for guiding filtrate to pass through the filtering surrounding barrels 5, a filter hole is arranged on the filtering surrounding barrels 5, the filtering residues on the filtering surrounding barrels 6 are also used for guiding the filter residues on the filter holes of the filtering surrounding barrels 5 to be separated from the filter holes, the circulating mechanism 7 is used for guiding the filter surrounding barrels 21 close to one side of the rotating shaft pipe 3 into the filtering surrounding barrels 21 in the filtering surrounding barrels 5, and the stirring rods 32 are arranged on the stirring rods 32; the rotary control mechanism 4 is used for controlling the rotary shaft tube 3 to rotate, the rotary shaft tube 3 drives the stirring rod 32 to rotate, raw materials in the feeding box 21 pass through the material guide hole 31 in the process and enter the enzymolysis reaction cavity for enzymolysis under the action of centrifugal force, filter residues in the feed liquid are filtered through the filter surrounding tube 5, and the generated collagen tripeptide feed liquid can pass through the filter surrounding tube 5.
In this embodiment, the filter enclosures 5 are sequentially arranged from inside to outside, and the pore diameters of the filter holes are sequentially reduced.
In this embodiment, a plurality of enclosing ring cavities with different radii are formed between the filtering enclosing barrels 5 with different radii, the filtrate drainage mechanism 6 includes a fixed ring 61 and a plurality of flat plate seats 64 respectively arranged above the separation and purification box 1, the fixed ring 61 is fixedly sleeved on the rotating shaft tube 3, radial struts 62 are installed on the fixed ring 61, pinch rollers 63 are rotatably installed at the outer ends of the struts 62, the flat plate seats 64 are circumferentially arranged and positioned below the circumferential rotation track of the pinch rollers 63, inclined plane blocks 68 for contacting the pinch rollers 63 are installed at the upper end of each flat plate seat 64, a first post rod 65 corresponding to the enclosing ring cavity is installed at the lower end of each flat plate seat 64, an arc-shaped plug block 66 corresponding to the enclosing ring cavity is installed at the lower end of each post rod 65, the arc-shaped plug blocks 66 are connected with the upper end of the separation and purification box 1 through a first spring 67, the side surfaces of the arc-shaped plug blocks 66 on the enclosing ring cavities are mutually attached, the filtering enclosing holes on the filtering enclosing barrels 5 are located below the arc-shaped plug blocks 66, and the inclined plane blocks 68 can be sequentially displaced downwards in the circumferential rotation process of the pinch rollers 63; specifically, in the process of rotating the rotary shaft tube 3, the fixing ring 61 can be driven to rotate, the fixing ring 61 drives the supporting rod 62 to rotate circumferentially, the supporting rod 62 drives the pressing wheel 63 to rotate circumferentially, meanwhile, the pressing wheel 63 can also rotate, when the pressing wheel 63 presses against the inclined plane block 68 to enable the inclined plane block 68 to move downwards, the inclined plane block 68 drives the arc-shaped block 66 to exert a pressing effect on local feed liquid below the arc-shaped block 66, so that local feed liquid at the position has a pressurizing impact effect on the filter holes on the inner side and the outer side of the filter peripheral cylinder, wherein the pressurizing impact on the filter holes on the inner side of the filter peripheral cylinder is beneficial to separating from the filter holes, the smoothness of the filter holes is kept, the pressurizing impact on the filter holes on the inner side of the filter peripheral cylinder is beneficial to improving the filter holes on the outer side of the filter peripheral cylinder, and the filter holes on the filter peripheral cylinder is beneficial to improving the filter holes on the inner side of the filter peripheral cylinder, and the filter holes on the filter peripheral cylinder is kept, and the filter holes on the filter peripheral cylinder is beneficial to the filter holes on the filter peripheral cylinder is kept from the filter holes on the filter peripheral cylinder; so that the arc-shaped plug block 66 continuously moves downwards and upwards, the feed liquid passing through the filter holes can be transmitted outwards to flow, and meanwhile, the effect of flushing the filter holes is achieved, and the smoothness of the filter holes is maintained; wherein, the downward movement distance of the arc-shaped chock 66 is smaller than the vertical thickness of the arc-shaped chock 66.
In the embodiment, a peripheral cavity is formed between the filtering surrounding cylinder 5 close to the outer side of the separation and purification box 1 and the separation and purification box 1, an annular plug 69 is mounted at the top of the peripheral cavity, a column groove 691 is formed at the upper end of the annular plug 69, a second pole 610 is connected to the column groove 691 in a sliding manner, the upper end of the second pole 610 is connected to the separation and purification box 1, and the annular plug 69 is connected with the upper end of the separation and purification box 1 through a second spring 611; specifically, the upper surface of the feed liquid at the peripheral cavity is in full contact with the lower end surface of the annular chock 69, and the upper surface of the feed liquid at the peripheral cavity is in full contact with the lower end surfaces of the arranged arc chocks 66, that is, in the process of downward movement and upward movement of the arc chocks 66, the liquid level of the partial feed liquid below the arc chocks 66 can be changed, and the annular chock 69 is driven by the second spring 611, so that the pressure effect on the upper surface of the feed liquid is maintained, and the compression effect on the partial feed liquid below the arc chocks 66 and the suction effect on the partial feed liquid below the arc chocks 66 are improved.
In this embodiment, the separation and purification box 1 is provided with a discharge pipe 11 which is communicated with the peripheral cavity.
In this embodiment, the cylindrical cavity of the filtering enclosure 5 near one side of the rotating shaft pipe 3 is set as an enzymolysis reaction cavity, the circulating drainage mechanism 7 includes a discharge pipe 71 communicated with the enzymolysis reaction cavity, the discharge pipe 71 includes a first cylindrical cavity 72 located at the upper part, a second cylindrical cavity 73 located at the middle part, and a third cylindrical cavity 74 located at the lower part, the lower end of the third cylindrical cavity 74 is connected with the feeding box 21 through a circulating pipe 78, a control pump 79 is installed on the circulating pipe 78, a first screw rod 75 is arranged in the first cylindrical cavity 72, the upper end of the first screw rod 75 is connected with the lower end of the rotating shaft pipe 3, a second screw rod 76 connected with the lower end of the first screw rod 75 is arranged in the second cylindrical cavity 73, a second screw rod 77 connected with the lower end of the second screw rod 76 is arranged in the third cylindrical cavity 74, the screw direction of the first screw rod 75 is opposite to the screw direction of the second screw rod 77, and the radius of the second screw rod 73 is larger than that of the first screw rod 72; that is, when the rotating shaft tube 3 rotates, the first screw rod 75, the column sleeve 76 and the second screw rod 77 are driven to rotate synchronously, wherein the first screw rod 75 has an upward guiding effect, so that the raw materials can better stay in the enzymolysis reaction chamber for enzymolysis reaction, the outer surface of the column sleeve 76 is of a smooth structure, the descending slag is not easy to flow upwards by the first screw rod 75, the second screw rod 77 has a downward guiding effect, and the mixed feed liquid and slag can be gathered and discharged into the circulation pipe 78.
In this embodiment, the distance between the outer edge of the first screw 75 and the wall of the first cavity 72 is equal to half the radius of the first cavity 72.
In this embodiment, the outer ring edge of the second screw rod 77 is attached to the wall of the third column cavity 74.
In this embodiment, the rotation control mechanism 4 includes a motor 41 mounted on the drum frame 2, a gear 42 is mounted at an output end of the motor 41, and the gear 42 is engaged with and connected with a toothed ring 43 sleeved on the rotating shaft tube 3.
In specific implementation, the method comprises the following steps:
Step one: treating livestock and poultry skin bones or fish skin scales as raw materials, preparing protease required by enzymolysis reaction, continuously introducing the protease into a feeding box 21 at a certain flow rate respectively, and opening a discharging pipe 11 to continuously discharge feed liquid from the discharging pipe 11 at a certain flow rate; after a certain amount of raw materials and protease are introduced into the separation and purification box 1 and reacted for a certain time, the discharging pipe 11 is opened at a certain flow rate, so that the enzymolysis reaction and the separation and purification of collagen tripeptide are synchronously performed, the separation and purification precision is improved, and the separation and purification efficiency is also improved;
Step two: starting a rotation control mechanism 4 to drive a rotary shaft tube 3 to rotate and stir the feed liquid, and simultaneously, driving a filtrate drainage mechanism 6 to guide filtrate to separate from a filter hole through a filter surrounding tube 5 and filter residues on a filter hole of the filter surrounding tube 5;
Step three: the material liquid in the filtering surrounding cylinder 5 near one side of the rotary shaft tube 3 is drained by the circulating drainage mechanism 7 to enter the feeding box 21, so that the circulating flow of the material liquid is formed.
The above description is only of the preferred embodiments of the invention, but the protection scope of the invention is not limited thereto, and any person skilled in the art who is skilled in the art to which the invention pertains should make equivalent substitutions or modifications according to the technical solution of the invention and its inventive concept within the scope of the invention.
Claims (6)
1. The utility model provides an adopt enzymolysis method separation and purification collagen tripeptide's equipment, includes separation and purification case (1), filtrate drainage mechanism (6) and circulation drainage mechanism (7), its characterized in that, separation and purification case (1) upper end is installed barrel head (2), barrel head (2) are gone up to rotate and are installed and stretch into inside pivot tube (3) of separation and purification case (1), be equipped with on pivot tube (3) pipe wall with inside guide hole (31) of intercommunication of separation and purification case (1), still install on barrel head (2) with feed case (21) that pivot tube (3) rotate and be connected, still install on barrel head (2) and be used for controlling pivot tube (3) pivoted rotary control mechanism (4), separation and purification case (1) are inside to be installed a plurality of different radiuses filter surrounding tube (5) concentrically, filtrate drainage mechanism (6) are used for guiding the filtrate to pass through filter surrounding tube (5), be equipped with the filtration pore on filter surrounding tube (5), just filtrate drainage mechanism (6) can guide filter tube (5) to break away from in the filter tube (7) and be used for entering in the filter tube (21) circulation drainage case (7), a stirring rod (32) for stirring is arranged on the rotating shaft tube (3);
A plurality of annular cavities with different radiuses are formed between a plurality of filtering enclosing barrels (5) with different radiuses, each filtrate drainage mechanism (6) comprises a fixed ring (61) and a plurality of flat plate seats (64) which are respectively arranged above a separation and purification box (1), the fixed ring (61) is fixedly sleeved on a rotating shaft pipe (3), radial supporting rods (62) are arranged on the fixed ring (61), pinch rollers (63) are rotatably arranged at the outer ends of the supporting rods (62), the flat plate seats (64) are circumferentially arranged and are positioned below the circumferential rotation track of the pinch rollers (63), inclined plane blocks (68) which are used for being in contact with the pinch rollers (63) are arranged at the upper ends of each flat plate seat (64), an arc-shaped plug block (66) which corresponds to the annular cavity is arranged at the lower end of each flat plate seat (64), the arc-shaped plug block (66) is connected with the upper end of the separation and purification box (1) through a spring (67), the arc-shaped plug blocks (66) can be sequentially arranged at the side surfaces of the annular plug blocks (66) which are in the downward direction of the rotation track of the filter drums (68;
A peripheral cavity is formed between the filtering surrounding cylinder (5) close to the outer side of the separation and purification box (1) and the separation and purification box (1), an annular plug block (69) is arranged at the top of the peripheral cavity, a column groove (691) is formed in the upper end of the annular plug block (69), a second pole (610) is connected to the column groove (691) in a sliding mode, the upper end of the second pole (610) is connected to the separation and purification box (1), and the annular plug block (69) is connected with the upper end of the separation and purification box (1) through a second spring (611);
a filtering surrounding cylinder (5) which is sequentially arranged from inside to outside, wherein the pore diameter of a filtering hole is sequentially reduced;
the utility model provides a filter enclosure (5) of being close to pivot tube (3) one side section of thick bamboo chamber is established to enzymolysis reaction chamber, circulation drainage mechanism (7) including row material pipe (71) with enzymolysis reaction chamber intercommunication, row material pipe (71) including be located post chamber one (72) on upper portion, post chamber two (73) in middle part and post chamber three (74) of lower part respectively, the lower extreme of post chamber three (74) is connected feeding case (21) through circulating pipe (78), install control pump (79) on circulating pipe (78), be equipped with spiral shell leaf pole one (75) in post chamber one (72), the upper end of spiral shell leaf pole one (75) is connected in the lower extreme of pivot tube (3), be equipped with in post chamber two (73) with spiral shell leaf pole two (77) that are connected with post chamber two (76) lower extreme, spiral shell leaf direction and spiral shell leaf pole two (77) of spiral shell leaf pole one (75) are in post chamber three (74), and the radius of spiral shell leaf pole two (72) in the opposite post chamber one.
2. The device for separating and purifying collagen tripeptide by adopting the enzymolysis method according to claim 1, wherein the separation and purification box (1) is provided with a discharge pipe (11) communicated with the peripheral cavity.
3. The apparatus for separating and purifying collagen tripeptide according to claim 2, wherein the distance between the outer rim of the first screw rod (75) and the wall of the first column cavity (72) is equal to half the radius of the first column cavity (72).
4. The device for separating and purifying collagen tripeptide by adopting the enzymolysis method according to claim 2, wherein the outer ring edge of the second spiral blade rod (77) is attached to the cavity wall of the third column cavity (74).
5. The device for separating and purifying collagen tripeptide by adopting the enzymolysis method according to claim 2, wherein the rotation control mechanism (4) comprises a motor (41) arranged on the barrel frame (2), a gear (42) is arranged at the output end of the motor (41), and the gear (42) is in meshed connection with a toothed ring (43) sleeved on the rotating shaft tube (3).
6. A process for separating and purifying collagen tripeptide by an enzymolysis method, which adopts the device for separating and purifying collagen tripeptide by an enzymolysis method according to any one of claims 3-5, and is characterized by comprising the following steps:
step one: treating livestock and poultry skin bones or fish skin scales as raw materials, preparing protease required by enzymolysis reaction, continuously introducing the protease into a feeding box (21) at a certain flow rate, and opening a discharging pipe (11) to continuously discharge feed liquid from the discharging pipe (11) at a certain flow rate;
Step two: starting a rotation control mechanism (4) to drive a rotary shaft tube (3) to rotate and stir feed liquid, and simultaneously, driving a filtrate drainage mechanism (6) to guide filtrate to separate from a filter hole through a filter surrounding tube (5) and filter residues on a filter hole of the filter surrounding tube (5);
Step three: the material liquid in the filtering surrounding cylinder (5) close to one side of the rotary shaft tube (3) is drained through the circulating drainage mechanism (7) to enter the feeding box (21), so that the circulating flow of the material liquid is formed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410961366.9A CN118496987B (en) | 2024-07-18 | 2024-07-18 | Equipment and process for separating and purifying collagen tripeptide by adopting enzymolysis method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410961366.9A CN118496987B (en) | 2024-07-18 | 2024-07-18 | Equipment and process for separating and purifying collagen tripeptide by adopting enzymolysis method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN118496987A CN118496987A (en) | 2024-08-16 |
| CN118496987B true CN118496987B (en) | 2024-10-18 |
Family
ID=92231460
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202410961366.9A Active CN118496987B (en) | 2024-07-18 | 2024-07-18 | Equipment and process for separating and purifying collagen tripeptide by adopting enzymolysis method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN118496987B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN119034451B (en) * | 2024-08-22 | 2025-01-28 | 平顶山天安煤业股份有限公司六矿 | Hydrogen sulfide gas treatment system for underground construction period of coal mine |
| CN119242437B (en) * | 2024-12-09 | 2025-03-14 | 安徽盛美诺生物技术有限公司 | Oyster peptide production device and oyster peptide production process |
| CN120733438A (en) * | 2025-09-08 | 2025-10-03 | 成都环境投资集团有限公司 | Biomass fermentation liquor separation and filtration treatment equipment and process |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117815753A (en) * | 2024-03-04 | 2024-04-05 | 德州蓝力生物技术有限公司 | A separation and purification device for fish collagen tripeptide and a separation and purification method thereof |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111893041A (en) * | 2020-08-07 | 2020-11-06 | 程红梅 | Collagen enzymolysis reaction device |
| CN213570520U (en) * | 2020-10-09 | 2021-06-29 | 海南盛美诺生物技术有限公司 | Fish skin enzymatic hydrolysis reaction kettle |
| CN113999883B (en) * | 2021-11-05 | 2024-01-30 | 地标(深圳)生物科技有限公司 | Production method of collagen tripeptide for skin repair |
| CN116440582B (en) * | 2023-06-13 | 2023-08-22 | 常州固钛科技有限公司 | Extraction and separation device |
| CN117165646B (en) * | 2023-08-25 | 2025-08-29 | 广州菲勒生物科技有限公司 | A collagen tripeptide composition and purification method thereof |
-
2024
- 2024-07-18 CN CN202410961366.9A patent/CN118496987B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117815753A (en) * | 2024-03-04 | 2024-04-05 | 德州蓝力生物技术有限公司 | A separation and purification device for fish collagen tripeptide and a separation and purification method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN118496987A (en) | 2024-08-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN118496987B (en) | Equipment and process for separating and purifying collagen tripeptide by adopting enzymolysis method | |
| CN220090760U (en) | Theanine's extraction element | |
| CN113210089A (en) | Raw material pretreatment device for hydrogen purification | |
| WO2022134129A1 (en) | Apparatus for extruding and molding plastic bottle cap | |
| CN214431437U (en) | Meatball forming device | |
| CN116099216A (en) | Molten salt dehydration degasser | |
| CN109770390B (en) | Sugarcane integrated processing device | |
| CN118046505A (en) | Preprocessing system for preparing high polymer plastic and application method thereof | |
| CN116099431A (en) | Solid raw material refining and mixing device for medical intermediate | |
| CN214600709U (en) | Cleaning device for collagen casing raw materials | |
| CN215140572U (en) | Environment remediation soil treatment equipment | |
| CN213756616U (en) | Mushroom belt cleaning device | |
| CN111714948A (en) | Marine engineering is with boats and ships sewage treatment device | |
| CN220034203U (en) | High-efficient biological contact oxidation unit | |
| CN118931705A (en) | A kind of equipment for producing and processing sauce | |
| CN219765314U (en) | Antiseized mixed reation kettle | |
| CN208392415U (en) | A kind of plastic bottle compression set | |
| CN216171922U (en) | High-efficiency vacuum casting stirring device | |
| CN114246245B (en) | Production process of wheat gluten special for wiredrawing protein | |
| CN119193314B (en) | Collagen tripeptide high-yield production and processing equipment | |
| CN222889698U (en) | Essence processing agitating unit | |
| CN223055636U (en) | But polypropylene production stirring reation kettle of self-cleaning | |
| CN119191664B (en) | Centrifugal filter press sludge dewatering device and method | |
| CN223474529U (en) | Raw material pretreatment device for lubricating oil blending | |
| CN220428935U (en) | Raw material cleaning device for processing clean products |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |