CN118222400A - Special culture dish for myocardial cells and nerve cells - Google Patents
Special culture dish for myocardial cells and nerve cells Download PDFInfo
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- CN118222400A CN118222400A CN202410466340.7A CN202410466340A CN118222400A CN 118222400 A CN118222400 A CN 118222400A CN 202410466340 A CN202410466340 A CN 202410466340A CN 118222400 A CN118222400 A CN 118222400A
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- 210000002569 neuron Anatomy 0.000 title claims abstract description 26
- 210000004027 cell Anatomy 0.000 title claims abstract description 25
- 230000002107 myocardial effect Effects 0.000 title claims abstract description 16
- 230000005484 gravity Effects 0.000 claims abstract description 8
- 230000007246 mechanism Effects 0.000 claims description 83
- 239000007788 liquid Substances 0.000 claims description 62
- 238000009434 installation Methods 0.000 claims description 14
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 12
- 210000004413 cardiac myocyte Anatomy 0.000 claims description 12
- 238000007789 sealing Methods 0.000 claims description 10
- 229910052742 iron Inorganic materials 0.000 claims description 6
- 235000015097 nutrients Nutrition 0.000 claims description 3
- 238000003491 array Methods 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 7
- 238000012258 culturing Methods 0.000 abstract description 5
- 239000012535 impurity Substances 0.000 abstract description 5
- 238000010586 diagram Methods 0.000 description 3
- 229910004298 SiO 2 Inorganic materials 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 239000012531 culture fluid Substances 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 208000019622 heart disease Diseases 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 210000000944 nerve tissue Anatomy 0.000 description 2
- 230000002269 spontaneous effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000033764 rhythmic process Effects 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
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- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
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- General Engineering & Computer Science (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a special culture dish for myocardial cells and nerve cells, and relates to the technical field of culture dishes. The special culture dish for myocardial cells and nerve cells comprises a bottom plate and also comprises: the U-shaped frame, U-shaped frame fixed connection is at the top of bottom plate, and the top of U-shaped frame is provided with the universal ball, the bottom fixedly connected with connecting rod of universal ball, and the lower extreme fixedly connected with gravity ball of connecting rod. The special culture dish for the myocardial cells and the nerve cells is convenient for shaking the culture dish body in all directions, and ensures the efficiency and the effect of culturing the myocardial cells and the nerve cells; the culture solution is convenient to replace, meanwhile, the quantitative addition of the culture solution is ensured, and the cover body is not required to be opened, so that a large amount of impurities in the outside air can be prevented from entering, and the quality of culture is ensured; the speed limiting treatment can be carried out on the flow rate of the added culture solution, so that the impact on the cells adsorbed on the inner wall of the dish body is avoided, and the quality of culture is ensured.
Description
Technical Field
The invention relates to the technical field of culture dishes, in particular to a special culture dish for myocardial cells and nerve cells.
Background
The culture dish is a laboratory vessel for culturing microorganisms or cells, can conveniently carry out the evolution of lecture organisms and the like, and has great significance in the research of heart diseases and the treatment of nerve tissues in the current culture of myocardial cells and nerve cells. The in vitro cultured myocardial cells have spontaneous rhythmicity and contractility, so that the culture of the myocardial cells becomes one of basic methods and means for heart disease research. The culture of nerve cells is of great significance to the treatment and research of damaged nerve tissues. In the traditional cell culture method, the culture dish generally adopts SiO 2 glass, the SiO 2 glass has stronger adsorptivity to cells, and myocardial cells have spontaneous rhythm and shrinkage characteristics and can grow only by firmly adsorbing on the surface of the culture dish, or else the growth is stopped.
However, current special culture dish of cardiomyocyte and nerve cell is when using, is inconvenient for rocking the culture dish, influences the quality of cultivateing, in the culture process, needs to change the culture solution, needs to use aseptic pipette to take out old culture solution and add new culture solution again, and convenient and fast inadequately simultaneously, needs to open the culture dish lid, and a large amount of impurity easily gets into in the outside air, causes the influence to the cultivation of cell to, the inner wall at the culture dish is impacted easily to the culture solution of adding, causes the break away of cell easily, influences the quality of cultivateing.
Disclosure of Invention
The invention aims to provide a special culture dish for myocardial cells and nerve cells, which solves the problems in the background technology.
In order to achieve the above purpose, the present invention provides the following technical solutions: a culture dish dedicated for cardiomyocytes and nerve cells, comprising a bottom plate, further comprising:
The device comprises a U-shaped frame, wherein the U-shaped frame is fixedly connected to the top of a bottom plate, the top of the U-shaped frame is provided with a universal ball, the bottom of the universal ball is fixedly connected with a connecting rod, the lower end of the connecting rod is fixedly connected with a gravity ball, the top of the universal ball is fixedly connected with a supporting rod, the upper end of the supporting rod is fixedly connected with a placing plate, the top of the placing plate is fixedly connected with a plurality of culture dish bodies arranged in an array manner, the culture dish bodies comprise dish bodies and cover bodies, the top of the bottom plate is fixedly connected with a supporting plate, the upper end of the supporting plate is fixedly connected with a liquid storage tank, the bottom of the liquid storage tank is fixedly connected with a liquid outlet pipe, the bottom of the liquid outlet pipe is fixedly connected with a quantitative box, the bottom of the quantitative box is fixedly connected with a connecting pipe, the bottom of the connecting pipe is fixedly connected with an installation box, the bottom of the installation box is fixedly connected with a conical pipe, the bottom of the conical pipe is fixedly connected with a fixing pipe, the side wall of the fixing pipe is fixedly connected with a plurality of liquid supply pipes arranged in an array manner, the other ends of the liquid supply pipes are fixedly inserted into the side walls of the dish bodies, the side walls of the dish bodies are fixedly provided with liquid supply pipes are fixedly connected with sealing plugs, and the liquid discharge thread sleeves are fixedly connected with the side plugs, and the liquid discharge thread sleeves are connected with the side plugs;
The first on-off mechanism is used for controlling the on-off of the liquid outlet pipe and is arranged on the side wall of the liquid outlet pipe;
The second switching mechanism is used for controlling the on-off of the liquid supply pipe and is arranged on the side wall of the liquid supply pipe;
The speed limiting mechanism is used for controlling the flow rate of the nutrient solution fed into the dish body and is arranged in the mounting box;
The shaking mechanism is used for pushing the culture dish body to shake and is arranged at the bottom of the U-shaped frame;
The limiting mechanism is used for limiting the universal ball and is arranged at the top of the U-shaped frame.
Preferably, the first on-off mechanism comprises a first fixed cover fixedly inserted into the side wall of the liquid outlet pipe, a first sliding plate is connected in the first fixed cover in a sliding mode, a first through hole is formed in the top of the first sliding plate, a first spring is fixedly connected between the first sliding plate and the first fixed cover, and the movement of the first sliding plate is pushed through the pushing mechanism.
Preferably, the second breaking mechanism comprises a second fixed cover fixedly inserted into the side wall of the liquid supply pipe, a second sliding plate is connected in the second fixed cover in a sliding mode, a second through hole is formed in the side wall of the second sliding plate, a second spring is fixedly connected between the second sliding plate and the second fixed cover, and a first pushing rod is fixedly connected to the top of the second sliding plate.
Preferably, the pushing mechanism comprises a pushing block fixedly connected to the upper end of the first pushing rod, the pushing block comprises a conical surface, the side wall of the first sliding plate is fixedly connected with a second pushing rod, and the other end of the second pushing rod is fixedly connected with a moving plate.
Preferably, the speed limiting mechanism comprises a rotating rod which is rotationally connected to the inner side wall of the installation box, a rotating fan is fixedly sleeved on the side wall of the rotating rod, a friction block which is arranged in an arc shape is connected to the side wall of the installation box through a reset mechanism, and the friction block is propped against the side wall of the rotating rod.
Preferably, the reset mechanism comprises a first fixed block fixedly connected to the side wall of the installation box, two symmetrically arranged first T-shaped guide rods are inserted into the side wall of the first fixed block, one end of each first T-shaped guide rod is fixed to the side wall of the friction block, and a third spring is sleeved on the side wall of each first T-shaped guide rod.
Preferably, the shaking mechanism comprises an L-shaped plate fixedly connected to the bottom of the U-shaped frame, a round hole is formed in the bottom of the L-shaped plate, a rotating ring is rotationally connected to the round hole, a gear ring is fixedly connected to the top of the rotating ring, a first bulge is fixedly connected to the inner side wall of the gear ring, a connecting rod is inserted into the round hole, a plurality of second bulges are fixedly connected to the side wall of the connecting rod, and the rotation of the gear ring is driven by the driving mechanism.
Preferably, the driving mechanism comprises a motor fixedly connected to the bottom of the L-shaped plate, the output end of the first motor is fixedly connected with a rotating shaft, and a gear is fixedly sleeved on the side wall of the rotating shaft.
Preferably, the limiting mechanism comprises a second fixed block fixedly connected to the top of the U-shaped frame, a limiting block is connected to the side wall of the second fixed block through a moving mechanism, and the limiting block abuts against the side wall of the universal ball.
Preferably, the moving mechanism comprises two symmetrically arranged second T-shaped guide rods inserted into the side walls of the second fixed block, one end of each second T-shaped guide rod is fixed with the side wall of the corresponding limiting block, a fourth spring is sleeved on the side wall of each second T-shaped guide rod, an electromagnet is fixedly connected to the side wall of each second fixed block, and an iron block is fixedly connected to the side wall of each limiting block.
Compared with the prior art, the invention has the beneficial effects that:
According to the culture dish special for the myocardial cells and the nerve cells, the shaking mechanism, the first on-off mechanism, the second on-off mechanism, the speed limiting mechanism and the like are arranged, so that shaking of the culture dish body in all directions is facilitated, and the efficiency and the effect of culturing the myocardial cells and the nerve cells are ensured; the culture solution is convenient to replace, meanwhile, the quantitative addition of the culture solution is ensured, and the cover body is not required to be opened, so that a large amount of impurities in the outside air can be prevented from entering, and the quality of culture is ensured; the speed limiting treatment can be carried out on the flow rate of the added culture solution, so that the impact on the cells adsorbed on the inner wall of the dish body is avoided, and the quality of culture is ensured.
Drawings
FIG. 1 is a schematic diagram of the overall structure of the present invention;
FIG. 2 is a schematic diagram of the position of the driving mechanism according to the present invention;
FIG. 3 is a schematic diagram showing the positions of the first and second switching mechanisms according to the present invention;
FIG. 4 is a schematic view of the position of the wobble mechanism according to the present invention;
FIG. 5 is an enlarged schematic view of the structure shown at A in FIG. 1;
FIG. 6 is an enlarged schematic view of the structure shown at B in FIG. 2;
FIG. 7 is an enlarged schematic view of FIG. 3C;
FIG. 8 is an enlarged schematic view of the structure of FIG. 3D;
FIG. 9 is an enlarged schematic view of the structure of FIG. 4 at E;
FIG. 10 is an enlarged schematic view of the structure shown at F in FIG. 7;
fig. 11 is an enlarged schematic view of the structure at G in fig. 9.
In the figure: 1. a bottom plate; 2. a first on-off mechanism; 201. a first fixed cover; 202. a first sliding plate; 203. a first through hole; 204. a first spring; 3. a second switching mechanism; 301. a second fixed cover; 302. a second sliding plate; 303. a second through hole; 304. a second spring; 305. a first push rod; 4. a pushing mechanism; 401. a second push rod; 402. a pushing block; 403. a conical surface; 404. a moving plate; 5. a speed limiting mechanism; 501. a rotating lever; 502. rotating the fan; 503. a friction block; 6. a reset mechanism; 601. a first fixed block; 602. a first T-shaped guide bar; 603. a third spring; 7. a moving mechanism; 701. a second T-shaped guide bar; 702. a fourth spring; 703. iron blocks; 704. an electromagnet; 8. a shaking mechanism; 801. an L-shaped plate; 802. a round hole; 803. a gear ring; 804. a second protrusion; 805. a first protrusion; 806. a rotating ring; 9. a driving mechanism; 901. a motor; 902. a rotating shaft; 903. a gear; 10. a limiting mechanism; 1001. a second fixed block; 1002. a limiting block; 11. a U-shaped frame; 12. a universal ball; 13. a connecting rod; 14. a gravity ball; 15. a support rod; 16. placing a plate; 17. a culture dish body; 1701. a dish body; 1702. a cover body; 18. a support plate; 19. a liquid storage tank; 20. a liquid outlet pipe; 21. a quantitative box; 22. a connecting pipe; 23. a mounting box; 24. a conical tube; 25. a fixed tube; 26. a liquid supply pipe; 27. a liquid discharge pipe; 28. a sealing plug; 29. and (5) a threaded sleeve.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to fig. 1 to 11, the present invention provides a technical solution: a culture dish dedicated for cardiomyocytes and nerve cells, comprising a bottom plate, further comprising:
The device comprises a U-shaped frame, wherein the U-shaped frame is fixedly connected to the top of a bottom plate, the top of the U-shaped frame is provided with a universal ball, the bottom of the universal ball is fixedly connected with a connecting rod, the lower end of the connecting rod is fixedly connected with a gravity ball, the top of the universal ball is fixedly connected with a supporting rod, the upper end of the supporting rod is fixedly connected with a placing plate, the top of the placing plate is fixedly connected with a plurality of culture dish bodies arranged in an array manner, the culture dish bodies comprise dish bodies and cover bodies, the top of the bottom plate is fixedly connected with a supporting plate, the upper end of the supporting plate is fixedly connected with a liquid storage tank, the bottom of the liquid storage tank is fixedly connected with a liquid outlet pipe, the bottom of the liquid outlet pipe is fixedly connected with a quantitative box, the bottom of the quantitative box is fixedly connected with a connecting pipe, the bottom of the connecting pipe is fixedly connected with an installation box, the bottom of the installation box is fixedly connected with a conical pipe, the bottom of the conical pipe is fixedly connected with a fixing pipe, the side wall of the fixing pipe is fixedly connected with a plurality of liquid supply pipes arranged in an array manner, the other ends of the liquid supply pipes are fixedly inserted into the side walls of the dish bodies, the side walls of the dish bodies are fixedly provided with liquid supply pipes are fixedly connected with sealing plugs, and the liquid discharge thread sleeves are fixedly connected with the side plugs, and the liquid discharge thread sleeves are connected with the side plugs;
The first on-off mechanism is used for controlling the on-off of the liquid outlet pipe and is arranged on the side wall of the liquid outlet pipe;
The second switching mechanism is used for controlling the on-off of the liquid supply pipe and is arranged on the side wall of the liquid supply pipe;
The speed limiting mechanism is used for controlling the flow rate of the nutrient solution fed into the dish body and is arranged in the mounting box;
The shaking mechanism is used for pushing the culture dish body to shake and is arranged at the bottom of the U-shaped frame;
The limiting mechanism is used for limiting the universal ball, is arranged at the top of the U-shaped frame, is convenient for shaking the culture dish body in all directions, and ensures the efficiency and effect of myocardial cell and nerve cell culture; the culture solution is convenient to replace, meanwhile, the quantitative addition of the culture solution is ensured, and the cover body is not required to be opened, so that a large amount of impurities in the outside air can be prevented from entering, and the quality of culture is ensured; the speed limiting treatment can be carried out on the flow rate of the added culture solution, so that the impact on the cells adsorbed on the inner wall of the dish body is avoided, and the quality of culture is ensured.
The first on-off mechanism comprises a first fixed cover fixedly inserted into the side wall of the liquid outlet pipe, a first sliding plate is connected in the first fixed cover in a sliding mode, a first through hole is formed in the top of the first sliding plate, a first spring is fixedly connected between the first sliding plate and the first fixed cover, the first sliding plate moves to push through a pushing mechanism, when culture fluid in a certain culture dish body needs to be replaced, old culture fluid in the dish body is firstly discharged through the liquid outlet pipe, after the discharge is completed, a sealing plug is plugged into the liquid outlet pipe again to seal the sealing plug, the pushing mechanism pushes the first sliding plate to slide in the first fixed cover, meanwhile, the first spring is compressed, the first through hole is staggered with the liquid outlet pipe, and at the moment, the first on-off mechanism is in an off state.
The second breaking mechanism comprises a second fixed cover fixedly inserted into the side wall of the liquid supply pipe, a second sliding plate is connected in the second fixed cover in a sliding mode, a second through hole is formed in the side wall of the second sliding plate, a second spring is fixedly connected between the second sliding plate and the second fixed cover, the top of the second sliding plate is fixedly connected with a first pushing rod, the second sliding plate is pushed to move downwards through the first pushing rod, meanwhile, the second spring is compressed, the second through hole is aligned with the liquid supply pipe, and at the moment, the second breaking mechanism is opened.
The pushing mechanism comprises a pushing block fixedly connected to the upper end of the first pushing rod, the pushing block comprises a conical surface, the side wall of the first sliding plate is fixedly connected with a second pushing rod, the other end of the second pushing rod is fixedly connected with a moving plate, the pushing block is pushed to move downwards, the moving plate slides on the conical surface, and the first sliding plate is pushed to slide into the first fixed cover through the second pushing rod.
The speed limiting mechanism comprises a rotating rod which is connected to the inner side wall of an installation box in a rotating way, a rotating fan is fixedly sleeved on the side wall of the rotating rod, a friction block which is arranged in an arc shape is connected to the side wall of the installation box through a reset mechanism, the friction block abuts against the side wall of the rotating rod, when quantitative culture solution in the quantitative box enters the installation box through a connecting pipe, the quantitative culture solution is impacted to rotate when blades of the rotating fan, the rotating fan rotates to drive the rotating rod to rotate, the friction block abuts against the side wall of the rotating rod under the action of the reset mechanism, so that speed limiting treatment can be carried out on rotation of the rotating rod, slow rotation of the rotating rod and the rotating fan is guaranteed, speed limiting treatment is carried out on the flow velocity of the culture solution, impact on cells adsorbed on the inner wall of a dish body is avoided, and accordingly the quality of culture is guaranteed.
The reset mechanism comprises a first fixed block fixedly connected to the side wall of the installation box, two symmetrically arranged first T-shaped guide rods are inserted into the side wall of the first fixed block, one end of each first T-shaped guide rod is fixed to the side wall of the friction block, a third spring is sleeved on the side wall of each first T-shaped guide rod, and the friction block is guaranteed to be propped against the side wall of the rotating rod all the time.
The shaking mechanism comprises an L-shaped plate fixedly connected to the bottom of the U-shaped frame, a round hole is formed in the bottom of the L-shaped plate, a rotating ring is rotationally connected to the round hole, a gear ring is fixedly connected to the top of the rotating ring, a first bulge is fixedly connected to the inner side wall of the gear ring, a connecting rod is inserted into the round hole, a plurality of second bulges are fixedly connected to the side wall of the connecting rod, the rotation of the gear ring is driven by a driving mechanism, the gear ring is driven to rotate by the driving mechanism, when the first bulge abuts against the side wall of the second bulge, the connecting rod is pushed to swing by a small extent along a universal ball, and the culture dish body is driven to swing by a supporting rod and a placing plate to form a shaking effect.
The driving mechanism comprises a motor fixedly connected to the bottom of the L-shaped plate, the output end of the first motor is fixedly connected with a rotating shaft, a gear is fixedly sleeved on the side wall of the rotating shaft, the motor is started, and the rotation of the motor drives the rotating shaft and the gear to rotate so as to drive the gear ring to rotate.
Stop gear includes the second fixed block of fixed connection at U-shaped frame top, the lateral wall of second fixed block is connected with the stopper through moving mechanism, and the stopper offsets with the lateral wall of universal ball, when needs shake the culture dish body, makes stopper and universal ball break away from through moving mechanism, at this moment, the universal ball can rotate, when stopping to shake, makes the culture dish body can be in the horizontality under the effect of gravity ball to, make the stopper offset with the lateral wall of universal ball under moving mechanism's effect, thereby avoid the culture dish body to rock at will, more reliable and stable.
The moving mechanism comprises a second T-shaped guide rod which is inserted into two symmetrical parts of the side wall of a second fixed block, one end of the second T-shaped guide rod is fixed with the side wall of a limiting block, a fourth spring is sleeved on the side wall of the second T-shaped guide rod, an electromagnet is fixedly connected with the side wall of the second fixed block, an iron block is fixedly connected with the side wall of the limiting block, the electromagnet is electrified, the iron block is attracted after the electromagnet is electrified, the limiting block is separated from a universal ball, meanwhile, the fourth spring is compressed, when the shaking is stopped, the culture dish body can be in a horizontal state under the action of the gravity ball, and the electromagnet is powered off, so that the limiting block is propped against the side wall of the universal ball under the action of the fourth spring.
Working principle: when in use, during culturing, firstly, strains and culture solution are added into the dish body, the cover body is covered, and then, the bottom plate and the culture dish body are integrally placed in a constant temperature incubator for culturing;
The electromagnet is electrified, the iron block is attracted after the electromagnet is electrified, so that the limiting block is separated from the universal ball, meanwhile, the fourth spring is compressed, then, the motor is started, the rotation of the motor drives the rotation shaft and the gear to rotate, and then the gear ring is driven to rotate;
When the shaking is stopped, the culture dish body can be in a horizontal state under the action of the gravity ball, and the electromagnet is powered off, so that the limiting block abuts against the side wall of the universal ball under the action of the fourth spring, the culture dish body is prevented from shaking at will, and the culture dish is more stable and reliable;
When the culture solution in a certain culture dish body needs to be replaced, the threaded sleeve is unscrewed, the sealing plug is screwed out of the liquid discharge pipe, and meanwhile, the culture dish body can be inclined to one side of the liquid discharge pipe through the shaking mechanism and the limiting mechanism, so that old culture solution in the dish body can be conveniently discharged through the liquid discharge pipe, and after the old culture solution is discharged, the sealing plug is plugged into the liquid discharge pipe again for sealing;
Then, the pushing block is pushed to move downwards, so that the moving plate slides on the conical surface, the first sliding plate is pushed to slide into the first fixed cover through the second pushing rod, meanwhile, the first spring is compressed, so that the first through hole is staggered with the liquid outlet pipe, at the moment, the liquid outlet pipe is plugged and sealed through the first sliding plate, meanwhile, the second sliding plate is pushed to move downwards through the first pushing rod, meanwhile, the second spring is compressed, so that the second through hole is aligned with the liquid supply pipe, at the moment, quantitative culture solution in the quantitative tank enters the dish body through the liquid supply pipe after passing through the connecting pipe, the mounting tank, the conical pipe and the fixed pipe, and moves and resets under the action of the second spring when the pushing block is loosened, so that the liquid supply pipe is plugged and sealed, the first sliding plate moves and resets under the action of the first spring, so that the first through hole is aligned with the liquid outlet pipe, at the moment, the culture solution in the tank enters the quantitative tank through the liquid outlet pipe for temporary storage, and is convenient for subsequent quantitative addition, the culture solution is convenient to replace, meanwhile, the quantitative addition of the culture solution is guaranteed, the quantitative addition of the culture solution is ensured, and the large external impurities can be avoided from entering the culture medium is ensured, and the large quality is avoided;
And, when quantitative culture solution in the quantitative case gets into the install bin through the connecting pipe, make it rotate when the blade of commentaries on classics fan, the rotation of commentaries on classics fan drives the rotation of dwang, make the clutch blocks offset with the lateral wall of dwang under the effect of third spring to can carry out the speed limit to the rotation of dwang and handle, guarantee that dwang and commentaries on classics fan carry out slow rotation, and then carry out the speed limit to the velocity of flow of culture solution and handle, avoid causing the impact to the cell that adsorbs on the inner wall of the dish body, thereby guarantee the quality of cultivateing.
Claims (10)
1. A special culture dish for myocardial cells and nerve cells, which comprises a bottom plate (1), and is characterized in that: further comprises:
U-shaped frame (11), U-shaped frame (11) fixed connection is at the top of bottom plate (1), and the top of U-shaped frame (11) is provided with universal ball (12), the bottom fixedly connected with connecting rod (13) of universal ball (12), and the lower extreme fixedly connected with gravity ball (14) of connecting rod (13), the top fixedly connected with bracing piece (15) of universal ball (12), and the upper end fixedly connected with of bracing piece (15) places board (16), the top fixedly connected with of placing board (16) is culture dish body (17) that a plurality of arrays set up, and culture dish body (17) include dish body (1701) and lid (1702), the top fixedly connected with backup pad (18) of bottom plate (1), and the upper end fixedly connected with liquid reserve tank (19) of backup pad (18), the bottom fixedly connected with drain pipe (20) of liquid reserve tank (19), and the bottom fixedly connected with ration case (21) of drain pipe (20), the bottom fixedly connected with connecting pipe (22) of ration case (21), and the bottom fixedly connected with connecting pipe (24) of bottom fixedly connected with conical tube (24) and conical tube (24), the side wall of the fixed pipe (25) is fixedly connected with a plurality of liquid supply pipes (26) which are arranged in an array, the other end of each liquid supply pipe (26) is fixedly inserted into the side wall of the dish body (1701), a liquid discharge pipe (27) is fixedly inserted into the side wall of the dish body (1701), a sealing plug (28) is inserted into the liquid discharge pipe (27), the end part of the sealing plug (28) is fixedly connected with a thread bush (29), and the thread bush (29) is in threaded connection with the side wall of the liquid discharge pipe (27);
The first on-off mechanism (2) is used for controlling the on-off of the liquid outlet pipe (20), and the first on-off mechanism (2) is arranged on the side wall of the liquid outlet pipe (20);
The second switching mechanism (3) is used for controlling the on-off of the liquid supply pipe (26), and the second switching mechanism (3) is arranged on the side wall of the liquid supply pipe (26);
A speed limiting mechanism (5), wherein the speed limiting mechanism (5) for controlling the flow rate of the nutrient solution fed into the dish body (1701) is arranged in the mounting box (23);
the shaking mechanism (8) is used for pushing the culture dish body (17) to shake, and the shaking mechanism (8) is arranged at the bottom of the U-shaped frame (11);
the limiting mechanism (10) is used for limiting the universal ball (12), and the limiting mechanism (10) is arranged at the top of the U-shaped frame (11).
2. A cardiomyocyte and nerve cell dedicated culture dish as claimed in claim 1 wherein: the first on-off mechanism (2) comprises a first fixed cover (201) fixedly inserted into the side wall of the liquid outlet pipe (20), a first sliding plate (202) is connected in the first fixed cover (201) in a sliding mode, a first through hole (203) is formed in the top of the first sliding plate (202), a first spring (204) is fixedly connected between the first sliding plate (202) and the first fixed cover (201), and the first sliding plate (202) is pushed through a pushing mechanism (4).
3. A cardiomyocyte and nerve cell dedicated culture dish as claimed in claim 1 wherein: the second breaking mechanism (3) comprises a second fixed cover (301) fixedly inserted into the side wall of the liquid supply pipe (26), a second sliding plate (302) is connected in the second fixed cover (301) in a sliding mode, a second through hole (303) is formed in the side wall of the second sliding plate (302), a second spring (304) is fixedly connected between the second sliding plate (302) and the second fixed cover (301), and a first pushing rod (305) is fixedly connected to the top of the second sliding plate (302).
4. A cardiomyocyte and nerve cell dedicated culture dish according to claim 3 wherein: the pushing mechanism (4) comprises a pushing block (402) fixedly connected to the upper end of the first pushing rod (305), the pushing block (402) comprises a conical surface (403), the side wall of the first sliding plate (202) is fixedly connected with a second pushing rod (401), and the other end of the second pushing rod (401) is fixedly connected with a moving plate (404).
5. A cardiomyocyte and nerve cell dedicated culture dish as claimed in claim 1 wherein: the speed limiting mechanism (5) comprises a rotating rod (501) which is rotationally connected to the inner side wall of the installation box (23), a rotating fan (502) is fixedly sleeved on the side wall of the rotating rod (501), a friction block (503) which is arranged in an arc shape is connected to the side wall of the installation box (23) through a reset mechanism (6), and the friction block (503) abuts against the side wall of the rotating rod (501).
6. A cardiomyocyte and nerve cell dedicated culture dish as claimed in claim 5 wherein: the reset mechanism (6) comprises a first fixed block (601) fixedly connected to the side wall of the installation box (23), two symmetrically arranged first T-shaped guide rods (602) are inserted into the side wall of the first fixed block (601), one end of each first T-shaped guide rod (602) is fixed to the side wall of the corresponding friction block (503), and a third spring (603) is sleeved on the side wall of each first T-shaped guide rod (602).
7. A cardiomyocyte and nerve cell dedicated culture dish as claimed in claim 1 wherein: the shaking mechanism (8) comprises an L-shaped plate (801) fixedly connected to the bottom of the U-shaped frame (11), a round hole (802) is formed in the bottom of the L-shaped plate (801), a rotating ring (806) is rotationally connected to the round hole (802), a gear ring (803) is fixedly connected to the top of the rotating ring (806), a first protrusion (805) is fixedly connected to the inner side wall of the gear ring (803), a connecting rod (13) is inserted into the round hole (802), a plurality of second protrusions (804) are fixedly connected to the side wall of the connecting rod (13), and the rotation of the gear ring (803) is driven by a driving mechanism (9).
8. A cardiomyocyte and nerve cell dedicated culture dish as claimed in claim 7 wherein: the driving mechanism (9) comprises a motor (901) fixedly connected to the bottom of the L-shaped plate (801), a rotating shaft (902) is fixedly connected to the output end of the first motor (901), and a gear (903) is fixedly sleeved on the side wall of the rotating shaft (902).
9. A cardiomyocyte and nerve cell dedicated culture dish as claimed in claim 1 wherein: the limiting mechanism (10) comprises a second fixed block (1001) fixedly connected to the top of the U-shaped frame (11), a limiting block (1002) is connected to the side wall of the second fixed block (1001) through a moving mechanism (7), and the limiting block (1002) abuts against the side wall of the universal ball (12).
10. A cardiomyocyte and nerve cell dedicated culture dish as claimed in claim 9 wherein: the moving mechanism (7) comprises two symmetrically arranged second T-shaped guide rods (701) inserted into the side walls of the second fixed block (1001), one end of each second T-shaped guide rod (701) is fixed with the side wall of each limiting block (1002), a fourth spring (702) is sleeved on the side wall of each second T-shaped guide rod (701), an electromagnet (704) is fixedly connected with the side wall of each second fixed block (1001), and an iron block (703) is fixedly connected with the side wall of each limiting block (1002).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410466340.7A CN118222400A (en) | 2024-04-18 | 2024-04-18 | Special culture dish for myocardial cells and nerve cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410466340.7A CN118222400A (en) | 2024-04-18 | 2024-04-18 | Special culture dish for myocardial cells and nerve cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN118222400A true CN118222400A (en) | 2024-06-21 |
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ID=91511076
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202410466340.7A Withdrawn CN118222400A (en) | 2024-04-18 | 2024-04-18 | Special culture dish for myocardial cells and nerve cells |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN118222400A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN119177167A (en) * | 2024-09-29 | 2024-12-24 | 河南省医学科学院 | Maintenance culture dish for myocardial slicing |
-
2024
- 2024-04-18 CN CN202410466340.7A patent/CN118222400A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN119177167A (en) * | 2024-09-29 | 2024-12-24 | 河南省医学科学院 | Maintenance culture dish for myocardial slicing |
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Application publication date: 20240621 |