CN1179634C - Technology for breeding male cytosterile line of hot pepper or pimento and preparing its seeds - Google Patents
Technology for breeding male cytosterile line of hot pepper or pimento and preparing its seeds Download PDFInfo
- Publication number
- CN1179634C CN1179634C CNB02125723XA CN02125723A CN1179634C CN 1179634 C CN1179634 C CN 1179634C CN B02125723X A CNB02125723X A CN B02125723XA CN 02125723 A CN02125723 A CN 02125723A CN 1179634 C CN1179634 C CN 1179634C
- Authority
- CN
- China
- Prior art keywords
- line
- pimento
- male
- male sterility
- cytoplasm
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Abstract
The present invention relates to a method for the selection of the cytoplasmic male sterile lines of hot pepper and green pepper, and for the reproduction of hybrid species of first generation with the utilization of the cytoplasmic male sterile lines of the hot pepper (the green pepper). The present invention adopts the female parent of the cytoplasmic male sterile line LANES of the green pepper, and the recurrent male parent of arietiform hot pepper No. 21 or lantern-shaped green pepper No. 8, and the cytoplasmic male sterile line 21A of the arietiform hot pepper or the cytoplasmic male sterile line 8A of the lantern-shaped green pepper is bred after 6 to 8 generations. According to experiments, with the utilization of the present invention for the reproduction of the hybrid seeds of the first generation, the production cost of the hybrid seeds is about half as the cost of conventional seed production by castration, and the purity of the hybrid seeds can reach 100%.
Description
Technical field:
The invention belongs to the heterosis utilization field, specifically the present invention relates to the seed selection of a kind of capsicum, pimento cytoplasm male sterility line and utilize the method for the numerous system first generation cross-fertilize seed of seed selection peppery (sweet) green pepper cytoplasm male sterility line.
Technical background:
Peppery (sweet) green pepper (Capscicum frutescens L.) has tangible hybrid vigour, but artificial emasculation pollination's production of hybrid seeds formality is numerous, the consumption cost, and the purity of the first generation of hybrid is difficult to guarantee that purity is identified also very time-consuming.Seed selection also utilizes male sterile line production first-filial generation seed, can simplify production of hybrid seeds formality, saving of labor's abridged edition, and can guarantee half-blooded seed purity.
Summary of the invention:
The objective of the invention is at the conventional breeding cost height of peppery (sweet) green pepper crossbreed, hybrid seed costliness and purity are difficult to guarantee this present situation at present, propose the selection and the seeding technique thereof of a kind of capsicum or pimento cytoplasm male sterility line, reach and utilize capsicum or the numerous system first cross of pimento cytoplasm male sterility line seed.
A kind of capsicum or pimento cytoplasm male sterility line selection, it is to be female parent with pimento cytoplasm male sterility line LANES, with No. 21 capsicums of erietiform or No. 8 pimentos of bird-caging is the samsara male parent, hybridization back is selected all flower stamens not have powder or only identifies through aceto-camine dyeing to carry out backcross transformation for the sterile strain of a small amount of abortion, lopsided pollen, breeds erietiform capsicum cytoplasm male sterility line 21A or bird-caging pimento cytoplasm male sterility line 8A through 6～8 generations; Described samsara male parent is the maintenance line of corresponding cytoplasm male sterility line.
Male sterile line 21A of the present invention and maintenance line 21B, male sterile line 8A and maintenance line 8B sow to produce male sterile line simultaneously with 2: 1 ratio; Its corresponding recovery of male sterile line 21A or 8A system begins sowing in good time with 2: 1 ratio, produces crossbreed.
In new peppery (sweet) green pepper cytoplasm male sterility line method of seed selection of the present invention, the samsara male parent is the maintenance line of corresponding cytoplasm male sterility line.
In new peppery (sweet) green pepper cytoplasm male sterility line method of seed selection of the present invention, in the backcross transformation process, the standard of perfection of sterile strain is: all flower stamens of complete stool do not have powder or identify through aceto-camine dyeing and only are a small amount of abortion, lopsided pollen.
The standard that the present invention breeds new capsicum or pimento cytoplasm male sterility line is that sterile rate and sterile degree all reach 100%.
It is female parent that the present invention also proposes a kind of cytoplasm male sterility line 21A with new seed selection, is for No. 3 that male parent (is 21A X LS with the Soviet Union green pepper
5) method of preparing hybrid kind.
The seed selection of capsicum of the present invention or pimento cytoplasm male sterility line and producing method for seed thereof, its detailed content is as follows:
1, seed selection capsicum or pimento cytoplasm male sterility line
With can genetic stability and not to be subjected to long bird-caging, the known pimento cytoplasm male sterility line LANES of environmental influences such as illumination, temperature be sterile source, and as maternal, be the samsara male parent with disease-resistant, high yield and No. 21 capsicums of known erietiform, No. 8 pimentos of bird-caging respectively, hybridize, obtain F
1The hybridization of Dai Zaiyu recurrent parent obtains BC
1Generation; At BC
1All flower stamens of selection complete stool do not have powder or identify the sterile strain that a small amount of abortion, lopsided pollen are only arranged through aceto-camine dyeing in generation, carry out backcross transformation with the samsara male parent, evaluation, selection through 6～8 generations, breed sterile rate and sterile degree and can both reach 100% and erietiform capsicum cytoplasm male sterility line 21A and bird-caging pimento cytoplasm male sterility line 8A that can genetic stability, its samsara male parent is respectively the maintenance line 21B of 21A and the maintenance line 8B of 8A.
2. utilize the method for capsicum cytoplasm male sterility line 21A and the numerous system first cross of pimento cytoplasm male sterility line 8A seed
(1) numerous system method of capsicum cytoplasm male sterility line 21A and pimento cytoplasm male sterility line 8A and corresponding maintenance line 21B and 8B
The selection fertility level is higher, the area that is fit to numerous kind of peppery (sweet) green pepper, adopt plastic tunnel or open country (isolated area is more than 1000 meters) the place of breeding as 21A and 8A, male sterile line 21A or 8A and maintenance line 21B or 8B sow simultaneously with 2: 1 ratio, male sterile line separates field planting with maintenance line and (refers to the male sterile line field planting together, suitably separate together) the maintenance line field planting, gather unopened big bud on half maintenance line plant flowering stage, preparation pollen, male sterile line is carried out artificial supplementary pollination, and second half maintenance line plant carries out gauze isolation selfing and reserves seed for planting.The Sheng phase topdresses as a result, in conjunction with prevention and elimination of disease and pests, in time gathers behind the fruit maturation and cuts open kind.The seed of fruit collection is required male-sterile seed on the male sterile line plant, and seed that fruit is gathered is the maintenance line seed on the gauze isolation maintenance line plant.
(2) utilize the method for capsicum cytoplasm male sterility line 21A and the numerous system first cross of pimento cytoplasm male sterility line 8A seed
The selection fertility is higher, the area that is fit to numerous kind of peppery (sweet) green pepper, adopt plastic tunnel or open country (isolated area is more than 1000 meters) numerous system place as the first cross seed, male sterile line 21A or 8A and recovery are the ratio with 2: 1, principle according to flower synchronization is begun sowing in good time, gather recovery flowering stage is unopened big bud on the plant, preparation pollen, male sterile line (female parent) plant is carried out artificial supplementary pollination, the Sheng phase topdresses as a result, in conjunction with prevention and elimination of disease and pests, look the upgrowth situation of plant and determine the quantity of bearing fruit, need not blowing after hybridization pollination finishes, in time gather from the male sterile line plant behind the fruit maturation and cut open kind, the seed of being gathered in the crops is the first cross seed of required numerous system.
The selection of capsicum provided by the invention or pimento cytoplasm male sterility line and seeding technique thereof are compared with existing conventional breeding and seeding technique has following advantage and good effect:
The present invention is that utilize can genetic stability and be not subjected to peppery (sweet) green pepper cytoplasm male sterility line that illumination, temperature environment influence to meet new peppery (sweet) green pepper cytoplasm male sterility line of breeding objective (demand), this method practical as sterile source by the method seed selection of backcross transformation.The present invention utilizes this method to breed capsicum cytoplasm male sterility line 21A and pimento cytoplasm male sterility line 8A, and new peppery (sweet) green pepper cytoplasm male sterility line of breeding is not subjected to illumination or Temperature Influence energy genetic stability, for new approach has been started in the heterosis, hybrid vigor utilization of peppery (sweet) green pepper.Utilize capsicum or the numerous system first cross of pimento cytoplasm male sterility line seed, its production cost only is about 50% of numerous kind of conventional castration, the purity of hybrid seed can reach 100%, the purity that does not need to carry out crossbreed is identified, thereby save time, help the sale of hybrid seed, owing to utilize capsicum or the numerous system hybrid seed of pimento cytoplasm male sterility line to save cost greatly, can reduce hybrid new breed seed price, thereby help the rapid popularization of new varieties, can make new varieties give play to the huge social benefit sooner.
Brief description of drawings:
Fig. 1 is erietiform capsicum and bird-caging pimento cytoplasm male sterility line 21A and the numerous system of 8A crossbreed ideograph
Embodiment:
No. 21 (fruit length is 11.79 centimetres with the erietiform capsicum, the fruit shoulder breadth is 2.3 centimetres, pulp is thick to be 2.09 millimeters, single fruit weight is 13.27 grams, have pungent) as the samsara male parent, LANES is a sterile source with bird-caging pimento cytoplasm male sterility line, as female parent, through the hybridization of 6 generations, backcross transformation, be tested and appraised, select, (fruit length is 11.77 centimetres, 2.56 centimetres of fruit shoulder breadths, thick 1.91 millimeters of pulp to breed new erietiform capsicum cytoplasm male sterility line 21A, single fruit weight 13.2 grams have pungent).Erietiform capsicum cytoplasm male sterility line 21A seed selection process and fruit properties see Table 1.
Table 1 erietiform capsicum cytoplasm male sterility line 21A seed selection process and fruit properties
The thick single fruit mouthfeel of the really long fruit shoulder breadth fruit shape index pulp of parent and transformation single fruit weight
From generation to generation (g) (cm) (cm) vertical/horizontal (mm) seed number pungent
LANES sterile source ♀ 120.5 13.28 6.29 2.11 3.93 121.7 is not peppery
No. 21 goat's horn green pepper ♂ 13.27 11.79 2.38 4.95 2.09 102.8 is peppery
F
137.6 12.99 3.59 3.62 2.50 130.3 is peppery slightly
BC
133.2 12.26 3.70 3.31 2.51 154.1 is peppery slightly
BC
222.0 11.61 3.01 3.86 2.05 95.6 is peppery slightly
BC
314.0 11.06 2.33 4.75 1.92 96.4 is peppery slightly
BC
114.4 11.67 2.34 4.99 1.78 71.8 is peppery
BC
513.2 11.77 2.56 4.60 1.91 90.3 is peppery
Annotate: numerical value is 10 fruit mean values in the table.
Cytoplasm male sterility line 21A with new seed selection is a female parent, surveys and has joined 289 peppery combinations of peppery X, wherein with 21A X LS
5(No. 3 A of Soviet Union green pepper) goat's horn green pepper is combined as the best, two annual yield increased Ningyangs green pepper volume increase 63.3%.This assembled growth gesture is strong, plant height 81cm, and the long 17.8cm of average fruit, fruit shoulder breadth 3.2cm, the thick 2.2mm of pulp, mean fruit weight 35.8g, tender fruit green, aging fruit scarlet, it is peppery, heat-resisting, heat-resisting to distinguish the flavor of, strong stress resistance.LS
3Not only recovery capability is strong, and with the coordinate force height of 21A, be a good recovery system.
Claims (2)
1. capsicum or pimento cytoplasm male sterility line selection, it is characterized in that with pimento cytoplasm male sterility line LANES be female parent, with No. 21 capsicums of erietiform and No. 8 pimentos of bird-caging is the samsara male parent, hybridization back is selected all flower stamens not have powder or only identifies through aceto-camine dyeing to carry out backcross transformation for the sterile strain of abortion, lopsided pollen, breeds new erietiform capsicum cytoplasm male sterility line 21A or bird-caging pimento cytoplasm male sterility line 8A through 6-8 generation; Described samsara male parent is the maintenance line of corresponding cytoplasm male sterility line.
2, the described cytoplasmic male sterilty producing method for seed of a kind of employing claim 1 is characterized in that male sterile line 21A and maintenance line 21B, male sterile line 8A and maintenance line 8B sow to produce male sterile line simultaneously with 2: 1 ratio; Male sterile line 21A or 8A and corresponding recovery system begin sowing in good time with 2: 1 ratio, produce crossbreed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB02125723XA CN1179634C (en) | 2002-08-14 | 2002-08-14 | Technology for breeding male cytosterile line of hot pepper or pimento and preparing its seeds |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB02125723XA CN1179634C (en) | 2002-08-14 | 2002-08-14 | Technology for breeding male cytosterile line of hot pepper or pimento and preparing its seeds |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1397158A CN1397158A (en) | 2003-02-19 |
| CN1179634C true CN1179634C (en) | 2004-12-15 |
Family
ID=4745650
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB02125723XA Expired - Fee Related CN1179634C (en) | 2002-08-14 | 2002-08-14 | Technology for breeding male cytosterile line of hot pepper or pimento and preparing its seeds |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1179634C (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102499054A (en) * | 2011-09-19 | 2012-06-20 | 潘德清 | Method for production of sweet pepper hybrid seeds |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100382672C (en) * | 2005-11-24 | 2008-04-23 | 四川大学 | Large piment secondary cultivating method |
| CN101011022B (en) * | 2007-01-30 | 2011-01-19 | 广东省农业科学院蔬菜研究所 | Seed selection method of capsicum variety |
| CN102511373B (en) * | 2011-11-02 | 2014-03-12 | 北京市农林科学院 | Lantern-shaped sweet pepper cytoplasmic male sterile line 09-313A and application of trilinear matched strain thereof in breeding |
| CN102783411B (en) * | 2012-08-23 | 2014-04-02 | 宝鸡市农业技术推广服务中心 | Bumble bee pollination seed production method of hot pepper cytoplasmic male sterile line |
| CN102870667A (en) * | 2012-10-12 | 2013-01-16 | 宝鸡市农业技术推广服务中心 | Open type cross breeding method of hot pepper cytoplasmic male sterile line |
| CN103026962A (en) * | 2012-12-11 | 2013-04-10 | 江苏省农业科学院 | Cultivation method of novel purple hot pepper variety |
| CN103250634B (en) * | 2013-05-29 | 2015-04-22 | 镇江瑞繁农艺有限公司 | Yellow leaf pepper, and breeding and propagation methods of cytoplasmic male sterile line of yellow leaf pepper |
| CN103843656A (en) * | 2014-04-04 | 2014-06-11 | 驻马店市农业科学院 | Transformation and hybrid seed cultivation method for oxhorn type chili male sterile dual-purpose line |
| CN104303987A (en) * | 2014-05-28 | 2015-01-28 | 安徽徽大农业有限公司 | High-yield hybrid seed production method for sweet pepper |
| CN104885921A (en) * | 2014-10-06 | 2015-09-09 | 安徽国盛农业科技有限责任公司 | High-yield hybrid seed production method for sweet peppers |
| CN108849483A (en) * | 2018-08-24 | 2018-11-23 | 贵州省蚕业研究所 | A kind of quick breeding method of capsicum CMS line |
| CN111758564A (en) * | 2020-07-23 | 2020-10-13 | 贵州省蚕业研究所(贵州省辣椒研究所) | Cluster breeding method for pepper sterile line |
-
2002
- 2002-08-14 CN CNB02125723XA patent/CN1179634C/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102499054A (en) * | 2011-09-19 | 2012-06-20 | 潘德清 | Method for production of sweet pepper hybrid seeds |
| CN102499054B (en) * | 2011-09-19 | 2013-08-14 | 慈溪市德清种子种苗有限公司 | Method for production of sweet pepper hybrid seeds |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1397158A (en) | 2003-02-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11246275B2 (en) | Method for breeding small-grain sterile rice line and simple method for producing hybrid rice seed | |
| CN1179634C (en) | Technology for breeding male cytosterile line of hot pepper or pimento and preparing its seeds | |
| JP2021000081A (en) | Method of simultaneously breeding capsicum male sterile line and homozygous restorer gene line by using recovery gene linkage marker | |
| CN102805029A (en) | Cultivation method of hybrid rapeseed capable of being planted at high altitude localities | |
| CN105981645A (en) | Efficient breeding method for early-maturing, high-yield and multi-resistant Brassica campestris L. | |
| CN1813523A (en) | Wheat interspecific distant hybrid, secondary backcross and selfing breeding method | |
| CN103355163A (en) | Breeding method of disease-resistant watermelon variety | |
| Muminov et al. | Analysis of yield and fiber quality traits in intraspecific and interspecific hybrids of cotton | |
| CN105145336B (en) | Breeding method for sunflower hybrid seed AR7-7376 with white outer shell | |
| CN103190336A (en) | Method for transforming wild cabbage into male sterility line of kohlrabi and breeding hybrids | |
| CN106962175B (en) | The method for creating of carpopodium impact resistance and the excellent Pumpkin Germplasm resource of Comprehensive Traits | |
| CN111183893B (en) | Novel breeding method of high-quality first-filial generation young Chinese flowering cabbage | |
| CN101969760A (en) | Cultivars of the poppy papaver bracteatum that produce seed capsules and thebaine in the first growing season | |
| CN1907010A (en) | Selection cross-breed method for pumpkin strain | |
| CN106962183B (en) | A kind of breeding method of 19 capsicum of Zheng green pepper | |
| CN103098708A (en) | Breeding method of erietiform chili inbred line | |
| CN100364385C (en) | Supergrade hybrid rice breeding parent matching method | |
| CN106577253A (en) | Cultivating method for high-starch high-yield sweet potatoes | |
| CN1054700A (en) | Technology for breeding high-yield and quality radish by hybridisation | |
| CN1836498A (en) | Corn cenospecies production method | |
| CN103688845A (en) | Breeding method for 50% male sterile line of oat | |
| JP2958457B1 (en) | Production of hybrid seeds of the first generation and production, maintenance and propagation of seed parent lines | |
| CN102511373B (en) | Lantern-shaped sweet pepper cytoplasmic male sterile line 09-313A and application of trilinear matched strain thereof in breeding | |
| CN1141017C (en) | Method for selectively breeding temperature-sensitive sterile line of wheat suitable for Yellow River-Huai River region in China | |
| CN101530058A (en) | Method for cultivating high-female few-seed watermelon |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |