CN117625394A - Litchi fungus strain culture solution, preparation method and culture method - Google Patents
Litchi fungus strain culture solution, preparation method and culture method Download PDFInfo
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- CN117625394A CN117625394A CN202311635924.4A CN202311635924A CN117625394A CN 117625394 A CN117625394 A CN 117625394A CN 202311635924 A CN202311635924 A CN 202311635924A CN 117625394 A CN117625394 A CN 117625394A
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- 241000233866 Fungi Species 0.000 title claims abstract description 74
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 238000012136 culture method Methods 0.000 title claims abstract description 7
- 241001629511 Litchi Species 0.000 title 1
- 229920002379 silicone rubber Polymers 0.000 claims abstract description 105
- 235000016709 nutrition Nutrition 0.000 claims abstract description 91
- 230000035764 nutrition Effects 0.000 claims abstract description 88
- 239000007788 liquid Substances 0.000 claims abstract description 60
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims abstract description 58
- 239000004945 silicone rubber Substances 0.000 claims abstract description 45
- 244000183278 Nephelium litchi Species 0.000 claims abstract description 43
- 239000006185 dispersion Substances 0.000 claims abstract description 39
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 38
- 235000015097 nutrients Nutrition 0.000 claims abstract description 35
- 241000256602 Isoptera Species 0.000 claims abstract description 33
- 239000003822 epoxy resin Substances 0.000 claims abstract description 32
- 229920000647 polyepoxide Polymers 0.000 claims abstract description 32
- 238000003756 stirring Methods 0.000 claims abstract description 31
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 29
- 239000008103 glucose Substances 0.000 claims abstract description 29
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims abstract description 29
- 235000019341 magnesium sulphate Nutrition 0.000 claims abstract description 29
- 235000019764 Soybean Meal Nutrition 0.000 claims abstract description 25
- 239000004455 soybean meal Substances 0.000 claims abstract description 25
- 229940049638 carbomer homopolymer type c Drugs 0.000 claims abstract description 22
- 229940043234 carbomer-940 Drugs 0.000 claims abstract description 22
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims abstract description 16
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 16
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract description 16
- 239000013530 defoamer Substances 0.000 claims abstract description 15
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims abstract description 15
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims abstract description 15
- 241000894006 Bacteria Species 0.000 claims abstract description 12
- 238000000034 method Methods 0.000 claims abstract description 11
- 239000006247 magnetic powder Substances 0.000 claims abstract description 9
- 238000002156 mixing Methods 0.000 claims description 34
- 230000001954 sterilising effect Effects 0.000 claims description 30
- 238000012258 culturing Methods 0.000 claims description 22
- 239000012528 membrane Substances 0.000 claims description 17
- NIXOWILDQLNWCW-UHFFFAOYSA-N Acrylic acid Chemical compound OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims description 16
- 229920002125 Sokalan® Polymers 0.000 claims description 16
- 229960001631 carbomer Drugs 0.000 claims description 16
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical group [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 14
- 238000001816 cooling Methods 0.000 claims description 14
- 239000000725 suspension Substances 0.000 claims description 14
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 13
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 13
- 239000002245 particle Substances 0.000 claims description 12
- 238000007873 sieving Methods 0.000 claims description 12
- 229910000859 α-Fe Inorganic materials 0.000 claims description 10
- 239000001963 growth medium Substances 0.000 claims description 9
- 239000000203 mixture Substances 0.000 claims description 8
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 7
- 238000001035 drying Methods 0.000 claims description 7
- 230000002093 peripheral effect Effects 0.000 claims description 7
- 238000011081 inoculation Methods 0.000 claims description 5
- 238000003860 storage Methods 0.000 claims description 3
- 239000011248 coating agent Substances 0.000 claims description 2
- 238000000576 coating method Methods 0.000 claims description 2
- 229920006334 epoxy coating Polymers 0.000 claims description 2
- 238000011218 seed culture Methods 0.000 claims 3
- 230000001580 bacterial effect Effects 0.000 claims 2
- 239000000243 solution Substances 0.000 description 83
- 239000002994 raw material Substances 0.000 description 20
- 238000010298 pulverizing process Methods 0.000 description 5
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 description 3
- 238000009630 liquid culture Methods 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 239000008223 sterile water Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 1
- 239000005708 Sodium hypochlorite Substances 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-N carbonic acid Chemical compound OC(O)=O BVKZGUZCCUSVTD-UHFFFAOYSA-N 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 229920001971 elastomer Polymers 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000021048 nutrient requirements Nutrition 0.000 description 1
- 235000006408 oxalic acid Nutrition 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 235000021251 pulses Nutrition 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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Abstract
The invention discloses a litchi fungus strain culture solution, a preparation method and a culture method, wherein the litchi fungus strain culture solution comprises a dispersion solution and a nutrition unit; the nutrition unit comprises the following components in parts by weight: 15-20 parts of water, 4-7 parts of silicone rubber, 0.05-0.1 part of soft magnetic powder, 0.04-0.1 part of epoxy resin, 1-3 parts of carbomer 940, 6-8 parts of glucose, 2-3 parts of soybean meal, 1-3 parts of termite fungus garden and 0.3-0.4 part of magnesium sulfate; the dispersion liquid comprises the following components in parts by weight: 0.05-0.1 part of DSA-5 defoamer, 0.1-0.15 part of monopotassium phosphate, 0.1-0.15 part of disodium hydrogen phosphate and the balance of water; the invention realizes quantitative replenishment of nutrient concentration of the strain culture solution and stirring of the strain culture solution, avoids the pollution of the culture solution caused by introducing foreign bacteria into an external stirring tool and additionally introduced culture solution in the culture process, and improves the success rate of culture.
Description
Technical Field
The invention relates to the technical field of biology, in particular to a litchi fungus strain culture solution, a preparation method and a culture method.
Background
In the prior art, a tissue separation method for preparing a litchi fungus mother strain is provided, wherein a liquid inoculation culture medium of an intermediate tissue is adopted for culturing the litchi fungus strain.
However, the amount of the dissolved nutrient substances in the liquid culture medium is fixed, and the nutrient substances in the liquid culture medium are gradually consumed and not supplemented along with the growth of the hyphae of the litchi fungus strains in the liquid culture medium, so that the concentration of the nutrient substances in the culture medium is gradually reduced, and finally the growth of the hyphae of the litchi fungus strains is restricted;
if the culture solution is supplemented into the culture container in the whole culture period, the pollution risk of the culture solution is greatly increased, and the success rate of culture is reduced.
Disclosure of Invention
The invention aims to overcome the defects and provide a litchi fungus strain culture solution, a preparation method and a culture method for improving the culture success rate.
In order to achieve the above object, the present invention is specifically as follows:
the first aspect of the invention provides a litchi fungus strain culture solution, which comprises a dispersion liquid and a nutrition unit; the nutrition unit comprises the following components in parts by weight: 15-20 parts of water, 4-7 parts of silicone rubber, 0.05-0.1 part of soft magnetic powder, 0.04-0.1 part of epoxy resin, 1-3 parts of carbomer 940, 6-8 parts of glucose, 2-3 parts of soybean meal, 1-3 parts of termite fungus garden and 0.3-0.4 part of magnesium sulfate;
the dispersion liquid comprises the following components in parts by weight: 0.05-0.1 part of DSA-5 defoamer, 0.1-0.15 part of monopotassium phosphate, 0.1-0.15 part of disodium hydrogen phosphate and the balance of water.
Further, the nutrition unit comprises a frame made of silicon rubber, elastic silicon rubber films are arranged on two opposite sides of the frame, the frame and the elastic silicon rubber films are enclosed to form a containing cavity, and a nutrition gel block is loaded in the containing cavity;
the inner surface of the elastic silicon rubber film is provided with a soft magnetic film layer consisting of soft magnetic powder, the peripheral wall of the frame body is provided with through holes, one elastic silicon rubber film is provided with elastic pieces at the periphery, two opposite elastic pieces are abutted against the inner side wall of the frame body and plug the corresponding through holes, the other two opposite elastic pieces are abutted against the outer side wall of the frame body and plug the corresponding through holes, and the elastic pieces and the through holes form a one-way valve structure; the outer surface of the elastic silicon rubber film is provided with a boss.
Further, the nutritional gel block is prepared by mixing carbomer, glucose, soybean meal, termite fungus gardens and magnesium sulfate.
Further, a gap is provided between the elastic silicone rubber film and the nutrient gel block.
Further, the soft magnetic powder is iron powder or soft magnetic ferrite.
The invention provides a preparation method of a litchi fungus strain culture solution, which comprises the following steps:
s1, preparing a nutrition gel block by 6-8 parts by weight of glucose, 2-3 parts by weight of soybean meal, 1-3 parts by weight of termite fungus gardens, 0.3-0.4 part by weight of magnesium sulfate and 1-3 parts by weight of carbomer 940;
s2, preparing 4-7 parts by weight of silicon rubber into an elastic silicon rubber film and a frame, mixing 0.05-0.1 part by weight of soft magnetic powder with 0.02-0.05 part by weight of epoxy resin, and coating the mixture on the inner surface of the elastic silicon rubber film to form a soft magnetic film layer;
s3, embedding the nutrition gel block into the frame, and attaching an elastic silicon rubber film with a soft magnetic film layer to the two side walls of the frame through 0.02-0.05 part by weight of epoxy resin to obtain a nutrition unit;
s4, adding 1000ml of water into 0.05-0.1 part by weight of DSA-5 defoamer, 0.1-0.15 part by weight of monopotassium phosphate and 0.1-0.15 part by weight of disodium hydrogen phosphate to dissolve to form dispersion liquid, and controlling the pH value to be within the range of 6.5-7.5; after the completion, adding the nutrition units into the dispersion liquid, and fully stirring to form a suspension liquid;
s5, placing the suspension into a sterilizing pot, sterilizing for 45min at 120-125 ℃, and cooling to room temperature to obtain the litchi fungus strain culture solution.
Further, the preparation of the nutritional gel block specifically comprises the following steps:
s11, respectively crushing termite fungus gardens, bean pulp and carbomer, and sieving the crushed termite fungus gardens, bean pulp and carbomer with a 100-mesh sieve;
s12, fully mixing glucose, soybean meal, termite fungus gardens and magnesium sulfate, adding 15-20 parts by weight of water after the mixing is completed, fully stirring to form a solution, putting the obtained solution into a sterilizing pot for sterilizing at 120-125 ℃ for 30min after the completion of the mixing, and cooling to obtain a nutrient solution;
s13, adding carbomer 940 into the nutrient solution, and fully stirring to form paste; after completion, the obtained paste is molded into blocks, and the nutrition gel blocks are obtained after drying.
The third aspect of the invention provides a method for culturing litchi fungus strains, which comprises the following steps:
a1, selecting robust fruiting bodies with the litchi bacteria maturity of 65-70%, and longitudinally cutting the fruiting bodies into fruiting body particles;
a2, adding the dispersion liquid into a culture container for storage, mixing and stirring the nutrition units and the fruiting body particles, separating the nutrition units after mixing, and adding the separated nutrition units into the culture container to finish inoculation of the mother seeds;
a3, placing the culture container in a concentration adjusting device, transferring the culture container into a constant temperature incubator for culturing for 1-2 days, observing whether contaminated bacteria are generated, and stopping culturing if the contaminated bacteria are generated;
otherwise, culturing for 3-4 days, observing whether fine granular hyphae are produced, and if so, detecting the sugar degree value of the culture solution;
if the sugar degree value is lower than the first preset value, adjusting the sugar degree value of the culture solution by a concentration adjusting device until the sugar degree value reaches the first preset value;
a4, culturing for 5-7 days, detecting whether the sugar degree value is lower than a second preset value, and if so, adjusting to the second preset value;
and A5, after culturing for 7 days, taking hypha, and then inoculating the hypha to a test tube culture medium, culturing for 7-10 days under the constant temperature condition, and then taking the hypha preferentially from the hypha to obtain the mother strain of the mother strain.
Further, the concentration adjusting device comprises a tray, a first electromagnet arranged at the top of the tray and a second electromagnet arranged at the bottom of the tray; the tray comprises tray bodies which are arranged at intervals up and down, and the tray bodies are fixedly connected through connecting columns, so that a space for accommodating the culture container is formed between the tray bodies.
The beneficial effects of the invention are as follows: according to the invention, the nutrition unit is introduced into the strain culture solution, and the magnetic fields generated by the soft magnetic film layer and the first electromagnet and the second electromagnet are matched, so that the quantitative supplementation of the nutrient concentration of the strain culture solution and the stirring of the strain culture solution are realized, the pollution of the culture solution caused by external stirring tools and the introduction of mixed bacteria in the additionally introduced culture solution in the culture process is avoided, the culture failure is avoided, and the success rate of the culture is improved.
Drawings
FIG. 1 is a schematic flow chart of the culturing litchi fungus strain;
FIG. 2 is a schematic diagram of the structure of the nutrition unit of the present invention;
figure 3 is a half-sectional view of the nutrition unit of the present invention;
FIG. 4 is an exploded schematic view of the nutrition unit of the present invention;
FIG. 5 is a schematic cross-sectional view of a concentration adjustment apparatus of the present invention;
FIG. 6 is a schematic view showing a structure in which a culture vessel is placed on a concentration adjusting apparatus according to the present invention;
reference numerals illustrate: 11. a frame; 12. an elastic silicone rubber film; 13. a nutrient gel block; 14. a soft magnetic film layer; 15. a through hole; 16. a spring plate; 17. a boss; 21. a tray; 22. a first electromagnet; 23. a second electromagnet; 3. a culture vessel.
Detailed Description
The invention will now be described in further detail with reference to the drawings and the specific embodiments, without limiting the scope of the invention.
Embodiment one: as shown in fig. 2 to 4, the litchi fungus strain culture solution in this embodiment includes a dispersion solution and a nutrition unit; the nutrition unit comprises the following components in parts by weight: 15 parts of water, 4 parts of silicone rubber, 0.05 part of iron powder, 0.04 part of epoxy resin, 1 part of carbomer 940, 6 parts of glucose, 2 parts of soybean meal, 1 part of termite fungus garden and 0.3 part of magnesium sulfate;
the dispersion liquid comprises the following components in parts by weight: 0.05DSA-5 defoamer, 0.1 part of monopotassium phosphate, 0.1 part of disodium hydrogen phosphate and the balance of water.
The nutrition unit comprises a frame 11 made of silicon rubber, elastic silicon rubber films 12 are fixedly arranged on the upper side and the lower side of the frame 11, the frame 11 and the elastic silicon rubber films 12 are enclosed to form a containing cavity, and a nutrition gel block 13 is loaded in the containing cavity; the nutrient gel block 13 is prepared by mixing carbomer, glucose, soybean meal, termite fungus garden and magnesium sulfate.
The inner surface of the elastic silicon rubber film 12 is provided with a soft magnetic film layer 14 composed of iron powder, through holes 15 are penetrated through the peripheral wall of the frame body, wherein elastic sheets 16 are arranged around one elastic silicon rubber film 12, two front and back opposite elastic sheets 16 are abutted against the inner side wall of the frame body and seal the corresponding through holes 15, the other two left and right opposite elastic sheets 16 are abutted against the outer side wall of the frame body and seal the corresponding through holes 15, the elastic sheets 16 abutted against the inner side wall of the frame body and the corresponding through holes 15 form a one-way valve structure for one-way liquid inlet, and the elastic sheets 16 abutted against the outer side wall of the frame body and the corresponding through holes 15 form a one-way valve structure for one-way liquid outlet; the outer surface of the elastic silicone rubber film 12 is uniformly provided with bosses 17 for attaching hyphae; a gap is provided between the elastic silicone rubber membrane 12 and the nutrient gel block 13 to provide a deformation space for the elastic silicone rubber membrane 12.
The preparation method of the litchi fungus strain culture solution in the embodiment 1 comprises the following steps:
step S1, preparing a nutrition gel block 13: the following raw materials are taken according to parts by weight: 6 parts of glucose, 2 parts of soybean meal, 1 part of termite fungus garden, 0.3 part of magnesium sulfate, 1 part of carbomer 940 and 15 parts of water;
respectively pulverizing termitid fungus garden, bean cake and carbomer, sieving with 100 mesh sieve, and controlling particle diameter within 0.12-0.15 mm; after sieving, fully mixing 6 parts of glucose, 2 parts of bean pulp, 1 part of termite fungus garden and 0.3 part of magnesium sulfate; adding 15 parts of water after mixing, fully stirring to form a solution, putting the obtained solution into a sterilizing pot for sterilizing at 120-125 ℃ for 30min after the completion of the mixing, cooling to obtain a nutrient solution, adding 1 part of carbomer 940 into the nutrient solution, and fully stirring to form a paste; after completion, the obtained paste is molded into blocks, and the nutrition gel blocks 13 are obtained after drying;
step S2, preparing the soft magnetic film layer 14, the elastic silicone rubber film 12 and the frame 11: the following raw materials are taken according to parts by weight: 4 parts of silicon rubber, 0.05 part of iron powder and 0.02 part of epoxy resin; 4 parts of silicon rubber is manufactured into a required elastic silicon rubber film 12 and a frame 11 by a mold forming process, and after 0.05 part of iron powder and 0.02 part of epoxy resin are mixed, the mixture is coated on the inner surface of the elastic silicon rubber film 12 to form a soft magnetic film layer 14;
step S3, preparing a nutrition unit: the following raw materials are taken according to parts by weight: embedding the nutrition gel block 13 prepared in the step S1 into the frame 11 prepared in the step S2 by 0.02 part of epoxy resin, and attaching and fixing the elastic silicone rubber film 12 with the soft magnetic film layer 14 prepared in the step S2 on the upper side wall and the lower side wall of the frame 11 through 0.02 part of epoxy resin after the completion of the step S2 to obtain a nutrition unit;
step S4, the following raw materials are taken according to parts by weight: 0.05 part of DSA-5 defoamer, 0.1 part of monopotassium phosphate and 0.1 part of disodium hydrogen phosphate, adding 1000ml of water to dissolve and form a dispersion liquid, and controlling the pH value to be in the range of 6.5-7.5; after the completion, adding the nutrition unit prepared in the step S3 into the dispersion liquid, and fully stirring to form suspension liquid;
in the step, potassium dihydrogen phosphate/disodium hydrogen phosphate is combined to form a pH buffer solution, and when acid metabolites such as oxalic acid, carbonic acid and the like generated in the growth and metabolism process of strains enter a dispersion solution system, the pH value of the dispersion solution system can be automatically compensated through the movement of hydrolysis balance, so that the pH value of the whole solution system is maintained within a preset range value;
and S5, placing the suspension prepared in the step S4 into a sterilizing pot, sterilizing for 45min at 120-125 ℃, and cooling to room temperature to obtain the litchi fungus strain culture solution.
Embodiment two: as shown in fig. 2 to 4, the litchi fungus strain culture solution in this embodiment includes a dispersion solution and a nutrition unit; the nutrition unit comprises the following components in parts by weight: 20 parts of water, 7 parts of silicone rubber, 0.1 part of soft magnetic ferrite, 0.03 part of epoxy resin, 3 parts of carbomer 940, 8 parts of glucose, 3 parts of soybean meal, 3 parts of termite fungus garden and 0.4 part of magnesium sulfate;
the dispersion liquid comprises the following components in parts by weight: 0.1DSA-5 defoamer, 0.15 part of monopotassium phosphate, 0.15 part of disodium hydrogen phosphate and the balance of water.
The nutrition unit comprises a frame 11 made of silicon rubber, elastic silicon rubber films 12 are fixedly arranged on the upper side and the lower side of the frame 11, the frame 11 and the elastic silicon rubber films 12 are enclosed to form a containing cavity, and a nutrition gel block 13 is loaded in the containing cavity; the nutrient gel block 13 is prepared by mixing carbomer, glucose, soybean meal, termite fungus garden and magnesium sulfate.
The inner surface of the elastic silicon rubber film 12 is provided with a soft magnetic film layer 14 composed of soft magnetic ferrite, through holes 15 are penetrated through the peripheral wall of the frame body, one elastic silicon rubber film 12 is provided with elastic pieces 16 around, two elastic pieces 16 which are opposite to each other from front to back are abutted against the inner side wall of the frame body and seal the corresponding through holes 15, the other two elastic pieces 16 which are opposite from left to right are abutted against the outer side wall of the frame body and seal the corresponding through holes 15, the elastic pieces 16 abutted against the inner side wall of the frame body and the corresponding through holes 15 form a one-way valve structure for one-way liquid inlet, and the elastic pieces 16 abutted against the outer side wall of the frame body and the corresponding through holes 15 form a one-way valve structure for one-way liquid outlet; the outer surface of the elastic silicone rubber film 12 is uniformly provided with bosses 17 for attaching hyphae; a gap is provided between the elastic silicone rubber membrane 12 and the nutrient gel block 13 to provide a deformation space for the elastic silicone rubber membrane 12.
The preparation method of the litchi fungus strain culture solution in the embodiment 2 comprises the following steps:
step S1, preparing a nutrition gel block 13: the following raw materials are taken according to parts by weight: 8 parts of glucose, 3 parts of soybean meal, 3 parts of termite fungus garden, 0.4 part of magnesium sulfate, 3 parts of carbomer 940 and 20 parts of water;
respectively pulverizing termitid fungus garden, bean cake and carbomer, sieving with 100 mesh sieve, and controlling particle diameter within 0.12-0.15 mm; after sieving, fully mixing 8 parts of glucose, 3 parts of bean pulp, 3 parts of termite fungus gardens and 0.4 part of magnesium sulfate; adding 20 parts of water after mixing, fully stirring to form a solution, putting the obtained solution into a sterilizing pot for sterilizing at 120-125 ℃ for 30min after the completion of the mixing, cooling to obtain a nutrient solution, adding 3 parts of carbomer 940 into the nutrient solution, and fully stirring to form a paste; after completion, the obtained paste is molded into blocks, and the nutrition gel blocks 13 are obtained after drying;
step S2, preparing the soft magnetic film layer 14, the elastic silicone rubber film 12 and the frame 11: the following raw materials are taken according to parts by weight: 7 parts of silicon rubber, 0.1 part of soft magnetic ferrite and 0.1 part of epoxy resin; 7 parts of silicon rubber is manufactured into a required elastic silicon rubber film 12 and a frame 11 by a mold forming process, and after 0.1 part by weight of soft magnetic ferrite and 0.05 part by weight of epoxy resin are mixed, the mixture is coated on the inner surface of the elastic silicon rubber film 12 to form a soft magnetic film layer 14;
step S3, preparing a nutrition unit: the following raw materials are taken according to parts by weight: embedding the nutrition gel block 13 prepared in the step S1 into the frame 11 prepared in the step S2 by 0.05 part of epoxy resin, and attaching and fixing the elastic silicone rubber film 12 with the soft magnetic film layer 14 prepared in the step S2 on the upper side wall and the lower side wall of the frame 11 through 0.05 part of epoxy resin after the completion of the step S2 to obtain a nutrition unit;
step S4, the following raw materials are taken according to parts by weight: 0.1 part of DSA-5 defoamer, 0.15 part of monopotassium phosphate and 0.15 part of disodium hydrogen phosphate, adding 1000ml of water to dissolve and form dispersion liquid, and controlling the pH value to be in the range of 6.5-7.5; after the completion, adding the nutrition unit prepared in the step S3 into the dispersion liquid, and fully stirring to form suspension liquid;
and S5, placing the suspension prepared in the step S4 into a sterilizing pot, sterilizing for 45min at 120-125 ℃, and cooling to room temperature to obtain the litchi fungus strain culture solution.
Embodiment III: as shown in fig. 2 to 4, the litchi fungus strain culture solution in this embodiment includes a dispersion solution and a nutrition unit; the nutrition unit comprises the following components in parts by weight: 17 parts of water, 5 parts of silicone rubber, 0.07 part of soft magnetic ferrite, 0.07 part of epoxy resin, 2 parts of carbomer 940, 7 parts of glucose, 2.5 parts of soybean meal, 2 parts of termite fungus garden and 0.35 part of magnesium sulfate;
the dispersion liquid comprises the following components in parts by weight: 0.07DSA-5 defoamer, 0.13 part of monopotassium phosphate, 0.13 part of disodium hydrogen phosphate and the balance of water.
The nutrition unit comprises a frame 11 made of silicon rubber, elastic silicon rubber films 12 are fixedly arranged on the upper side and the lower side of the frame 11, the frame 11 and the elastic silicon rubber films 12 are enclosed to form a containing cavity, and a nutrition gel block 13 is loaded in the containing cavity; the nutrient gel block 13 is prepared by mixing carbomer, glucose, soybean meal, termite fungus garden and magnesium sulfate.
The inner surface of the elastic silicon rubber film 12 is provided with a soft magnetic film layer 14 composed of soft magnetic ferrite, through holes 15 are penetrated through the peripheral wall of the frame body, one elastic silicon rubber film 12 is provided with elastic pieces 16 around, two elastic pieces 16 which are opposite to each other from front to back are abutted against the inner side wall of the frame body and seal the corresponding through holes 15, the other two elastic pieces 16 which are opposite from left to right are abutted against the outer side wall of the frame body and seal the corresponding through holes 15, the elastic pieces 16 abutted against the inner side wall of the frame body and the corresponding through holes 15 form a one-way valve structure for one-way liquid inlet, and the elastic pieces 16 abutted against the outer side wall of the frame body and the corresponding through holes 15 form a one-way valve structure for one-way liquid outlet; the outer surface of the elastic silicone rubber film 12 is uniformly provided with bosses 17 for attaching hyphae; a gap is provided between the elastic silicone rubber membrane 12 and the nutrient gel block 13 to provide a deformation space for the elastic silicone rubber membrane 12.
The preparation method of the litchi fungus strain culture solution in the embodiment 3 comprises the following steps:
step S1, preparing a nutrition gel block 13: the following raw materials are taken according to parts by weight: 7 parts of glucose, 2.5 parts of soybean meal, 2 parts of termite fungus garden, 0.35 part of magnesium sulfate, 2 parts of carbomer 940 and 17 parts of water;
respectively pulverizing termitid fungus garden, bean cake and carbomer, sieving with 100 mesh sieve, and controlling particle diameter within 0.12-0.15 mm; after sieving, fully mixing 7 parts of glucose, 2.5 parts of bean pulp, 2 parts of termite fungus gardens and 0.35 part of magnesium sulfate; adding 17 parts of water after mixing, fully stirring to form a solution, putting the obtained solution into a sterilizing pot for sterilizing at 120-125 ℃ for 30min after the completion of the mixing, cooling to obtain a nutrient solution, adding 2 parts of carbomer 940 into the nutrient solution, and fully stirring to form a paste; after completion, the obtained paste is molded into blocks, and the nutrition gel blocks 13 are obtained after drying;
step S2, preparing the soft magnetic film layer 14, the elastic silicone rubber film 12 and the frame 11: the following raw materials are taken according to parts by weight: 5 parts of silicon rubber, 0.07 part of soft magnetic ferrite and 0.07 part of epoxy resin; 5 parts of silicon rubber is manufactured into a required elastic silicon rubber film 12 and a frame 11 by a mold forming process, and after 0.07 part by weight of soft magnetic ferrite and 0.035 part by weight of epoxy resin are mixed, the mixture is coated on the inner surface of the elastic silicon rubber film 12 to form a soft magnetic film layer 14;
step S3, preparing a nutrition unit: the following raw materials are taken according to parts by weight: 0.035 parts of epoxy resin, embedding the nutrition gel block 13 prepared in the step S1 into the frame 11 prepared in the step S2, and after the completion, attaching and fixing the elastic silicone rubber film 12 with the soft magnetic film layer 14 prepared in the step S2 on the upper side wall and the lower side wall of the frame 11 through 0.035 parts of epoxy resin to obtain a nutrition unit;
step S4, the following raw materials are taken according to parts by weight: 0.07 part of DSA-5 defoamer, 0.13 part of monopotassium phosphate and 0.13 part of disodium hydrogen phosphate, adding 1000ml of water to dissolve and form a dispersion liquid, and controlling the pH value to be in the range of 6.5-7.5; after the completion, adding the nutrition unit prepared in the step S3 into the dispersion liquid, and fully stirring to form suspension liquid;
and S5, placing the suspension prepared in the step S4 into a sterilizing pot, sterilizing for 45min at 120-125 ℃, and cooling to room temperature to obtain the litchi fungus strain culture solution.
Embodiment four: as shown in fig. 2 to 4, the litchi fungus strain culture solution in this embodiment includes a dispersion solution and a nutrition unit; the nutrition unit comprises the following components in parts by weight: 19 parts of water, 6 parts of silicone rubber, 0.08 part of iron powder, 0.09 part of epoxy resin, 3 parts of carbomer 940, 8 parts of glucose, 2 parts of soybean meal, 2 parts of termite fungus garden and 0.38 part of magnesium sulfate;
the dispersion liquid comprises the following components in parts by weight: 0.09DSA-5 defoamer, 0.14 part of monopotassium phosphate, 0.14 part of disodium hydrogen phosphate and the balance of water.
The nutrition unit comprises a frame 11 made of silicon rubber, elastic silicon rubber films 12 are fixedly arranged on the upper side and the lower side of the frame 11, the frame 11 and the elastic silicon rubber films 12 are enclosed to form a containing cavity, and a nutrition gel block 13 is loaded in the containing cavity; the nutrient gel block 13 is prepared by mixing carbomer, glucose, soybean meal, termite fungus garden and magnesium sulfate.
The inner surface of the elastic silicon rubber film 12 is provided with a soft magnetic film layer 14 composed of iron powder, through holes 15 are penetrated through the peripheral wall of the frame body, wherein elastic sheets 16 are arranged around one elastic silicon rubber film 12, two front and back opposite elastic sheets 16 are abutted against the inner side wall of the frame body and seal the corresponding through holes 15, the other two left and right opposite elastic sheets 16 are abutted against the outer side wall of the frame body and seal the corresponding through holes 15, the elastic sheets 16 abutted against the inner side wall of the frame body and the corresponding through holes 15 form a one-way valve structure for one-way liquid inlet, and the elastic sheets 16 abutted against the outer side wall of the frame body and the corresponding through holes 15 form a one-way valve structure for one-way liquid outlet; the outer surface of the elastic silicone rubber film 12 is uniformly provided with bosses 17 for attaching hyphae; a gap is provided between the elastic silicone rubber membrane 12 and the nutrient gel block 13 to provide a deformation space for the elastic silicone rubber membrane 12.
The preparation method of the litchi fungus strain culture solution in the embodiment 4 comprises the following steps:
step S1, preparing a nutrition gel block 13: the following raw materials are taken according to parts by weight: 8 parts of glucose, 2 parts of soybean meal, 2 parts of termite fungus garden, 0.38 part of magnesium sulfate, 3 parts of carbomer 940 and 19 parts of water;
respectively pulverizing termitid fungus garden, bean cake and carbomer, sieving with 100 mesh sieve, and controlling particle diameter within 0.12-0.15 mm; after sieving, fully mixing 8 parts of glucose, 2 parts of bean pulp, 2 parts of termite fungus gardens and 0.38 part of magnesium sulfate; adding 19 parts of water after mixing, fully stirring to form a solution, putting the obtained solution into a sterilizing pot for sterilizing at 120-125 ℃ for 30min after the completion of the mixing, cooling to obtain a nutrient solution, adding 3 parts of carbomer 940 into the nutrient solution, and fully stirring to form a paste; after completion, the obtained paste is molded into blocks, and the nutrition gel blocks 13 are obtained after drying;
step S2, preparing the soft magnetic film layer 14, the elastic silicone rubber film 12 and the frame 11: the following raw materials are taken according to parts by weight: 6 parts of silicone rubber, 0.08 part of iron powder and 0.09 part of epoxy resin; 6 parts of silicon rubber is manufactured into a required elastic silicon rubber film 12 and a frame 11 by a mold forming process, and after 0.08 part by weight of iron powder and 0.045 part by weight of epoxy resin are mixed, the mixture is coated on the inner surface of the elastic silicon rubber film 12 to form a soft magnetic film layer 14;
step S3, preparing a nutrition unit: the following raw materials are taken according to parts by weight: embedding the nutrition gel block 13 prepared in the step S1 into the frame 11 prepared in the step S2 by 0.045 part of epoxy resin, and attaching and fixing the elastic silicone rubber film 12 with the soft magnetic film layer 14 prepared in the step S2 on the upper side wall and the lower side wall of the frame 11 through 0.045 part of epoxy resin after the completion of the step S2 to obtain a nutrition unit;
step S4, the following raw materials are taken according to parts by weight: 0.09 part of DSA-5 defoamer, 0.14 part of monopotassium phosphate and 0.14 part of disodium hydrogen phosphate, adding 1000ml of water to dissolve and form a dispersion liquid, and controlling the pH value to be in the range of 6.5-7.5; after the completion, adding the nutrition unit prepared in the step S3 into the dispersion liquid, and fully stirring to form suspension liquid;
and S5, placing the suspension prepared in the step S4 into a sterilizing pot, sterilizing for 45min at 120-125 ℃, and cooling to room temperature to obtain the litchi fungus strain culture solution.
Fifth embodiment: as shown in fig. 2 to 4, the litchi fungus strain culture solution in this embodiment includes a dispersion solution and a nutrition unit; the nutrition unit comprises the following components in parts by weight: 17.5 parts of water, 6 parts of silicone rubber, 0.08 part of iron powder, 0.07 part of epoxy resin, 2.5 parts of carbomer 940, 6.5 parts of glucose, 2.5 parts of soybean meal, 1.5 parts of termite fungus garden and 0.35 part of magnesium sulfate;
the dispersion liquid comprises the following components in parts by weight: 0.08DSA-5 defoamer, 0.15 part of monopotassium phosphate, 0.14 part of disodium hydrogen phosphate and the balance of water.
The nutrition unit comprises a frame 11 made of silicon rubber, elastic silicon rubber films 12 are fixedly arranged on the upper side and the lower side of the frame 11, the frame 11 and the elastic silicon rubber films 12 are enclosed to form a containing cavity, and a nutrition gel block 13 is loaded in the containing cavity; the nutrient gel block 13 is prepared by mixing carbomer, glucose, soybean meal, termite fungus garden and magnesium sulfate.
The inner surface of the elastic silicon rubber film 12 is provided with a soft magnetic film layer 14 composed of iron powder, through holes 15 are penetrated through the peripheral wall of the frame body, wherein elastic sheets 16 are arranged around one elastic silicon rubber film 12, two front and back opposite elastic sheets 16 are abutted against the inner side wall of the frame body and seal the corresponding through holes 15, the other two left and right opposite elastic sheets 16 are abutted against the outer side wall of the frame body and seal the corresponding through holes 15, the elastic sheets 16 abutted against the inner side wall of the frame body and the corresponding through holes 15 form a one-way valve structure for one-way liquid inlet, and the elastic sheets 16 abutted against the outer side wall of the frame body and the corresponding through holes 15 form a one-way valve structure for one-way liquid outlet; the outer surface of the elastic silicone rubber film 12 is uniformly provided with bosses 17 for attaching hyphae; a gap is provided between the elastic silicone rubber membrane 12 and the nutrient gel block 13 to provide a deformation space for the elastic silicone rubber membrane 12.
The preparation method of the litchi fungus strain culture solution in the embodiment 5 comprises the following steps:
step S1, preparing a nutrition gel block 13: the following raw materials are taken according to parts by weight: 6.5 parts of glucose, 2.5 parts of soybean meal, 1.5 parts of termite fungus garden, 0.35 part of magnesium sulfate, 2.5 parts of carbomer 940 and 17.5 parts of water;
respectively pulverizing termitid fungus garden, bean cake and carbomer, sieving with 100 mesh sieve, and controlling particle diameter within 0.12-0.15 mm; after sieving, fully mixing 6.5 parts of glucose, 2.5 parts of soybean meal, 1.5 parts of termite fungus garden and 0.35 part of magnesium sulfate; adding 17.5 parts of water after the completion of mixing, fully stirring to form a solution, putting the obtained solution into a sterilizing pot for sterilizing at 120-125 ℃ for 30min after the completion of the mixing, cooling to obtain a nutrient solution, adding 2.5 parts of carbomer 940 into the nutrient solution, and fully stirring to form a paste; after completion, the obtained paste is molded into blocks, and the nutrition gel blocks 13 are obtained after drying;
step S2, preparing the soft magnetic film layer 14, the elastic silicone rubber film 12 and the frame 11: the following raw materials are taken according to parts by weight: 6 parts of silicone rubber, 0.08 part of iron powder and 0.07 part of epoxy resin; 6 parts of silicon rubber is manufactured into a required elastic silicon rubber film 12 and a frame 11 by a mold forming process, and after 0.08 part of iron powder and 0.035 part of epoxy resin are mixed, the mixture is coated on the inner surface of the elastic silicon rubber film 12 to form a soft magnetic film layer 14;
step S3, preparing a nutrition unit: the following raw materials are taken according to parts by weight: 0.035 parts of epoxy resin, embedding the nutrition gel block 13 prepared in the step S1 into the frame 11 prepared in the step S2, and after the completion, attaching and fixing the elastic silicone rubber film 12 with the soft magnetic film layer 14 prepared in the step S2 on the upper side wall and the lower side wall of the frame 11 through 0.035 parts of epoxy resin to obtain a nutrition unit;
step S4, the following raw materials are taken according to parts by weight: 0.08 part of DSA-5 defoamer, 0.15 part of monopotassium phosphate and 0.14 part of disodium hydrogen phosphate, adding 1000ml of water to dissolve and form a dispersion liquid, and controlling the pH value to be in the range of 6.5-7.5; after the completion, adding the nutrition unit prepared in the step S3 into the dispersion liquid, and fully stirring to form suspension liquid;
and S5, placing the suspension prepared in the step S4 into a sterilizing pot, sterilizing for 45min at 120-125 ℃, and cooling to room temperature to obtain the litchi fungus strain culture solution.
As shown in fig. 1 to 6, this embodiment also provides a method for culturing a litchi fungus strain by using the litchi fungus strain culture solution prepared in the above embodiment, including the following steps:
step A1, obtaining fruiting body particles: selecting strong fruiting bodies with the maturity of 65-70% of litchi bacteria, removing macroscopic impurities, washing with sterile water, airing surface moisture, soaking for 45-60 s with 2.5% -3% hydrogen peroxide solution, washing with sterile water for 3 times, soaking for 25-35min with 1.5-2.5% sodium hypochlorite solution, washing with sterile water for 3 times, sucking surface moisture, cutting the fruiting bodies with a sterile scalpel, and longitudinally cutting into fruiting body particles with the thickness of 3mm multiplied by 3 mm;
step A2, inoculating the mother seeds: passing the strain culture solution through a sterilizing screen with the aperture of 2mm to separate the nutrition unit from the dispersion liquid; after the separation is completed, the separated dispersion liquid is added into a culture container 3 for storage, and the separated nutrition units and fruiting body particles are moved into a stirrer and mixed and stirred for 15 minutes at a rotating speed of 50 r/min; after the completion, the mixture passes through a sterilization screen mesh with the aperture of 2.5mm, the nutrition units are separated, the nutrition units are added into a culture container 3 which stores dispersion liquid after the separation, and the nutrition units are fully dispersed in the dispersion liquid by stirring, so that the inoculation of mother seeds is completed;
step A3, after the step A2 is completed, placing the culture container 3 in a concentration adjusting device, then transferring the culture container into a constant temperature incubator, culturing for 1-2 days at 25 ℃, observing whether contaminated bacteria are generated on the liquid surface of the culture solution and the wall of the culture container 3, and if the contaminated bacteria are found, stopping culturing the sample, avoiding ineffective culturing and reducing loss;
otherwise, culturing for 3-4 days, observing whether fine granular hypha is generated in the culture container 3, if so, taking the culture solution to detect the sugar content, and controlling the sugar degree value of the culture container 3 to be within the range of 1.8-2.2 ℃ and the sugar degree value to be within the range of 1.8-2.2 ℃ to be a first preset value;
if the sugar degree value is lower than the first preset value, adjusting the sugar degree value of the culture solution by a concentration adjusting device until the sugar degree value reaches the first preset value;
step A4, culturing for 5-7 days, wherein the growth speed of hyphae is increased, the sugar degree value of the culture solution is controlled to be in the range of 2.2-2.5 ℃ as the nutrient requirement is increased, the sugar degree value is controlled to be a second preset value in the range of 2.2-2.5 ℃, whether the sugar degree value is lower than the second preset value is detected, and if the sugar degree value is lower than the second preset value, the sugar degree value of the culture solution is adjusted to the second preset value through a concentration adjusting device;
step A5, selecting hyphae with pure, strong and normal color and luster and without interruption, connecting a culture medium in an inoculation box to hook the hyphae, and connecting the hyphae to another test tube culture medium; culturing at constant temperature of 23-25deg.C for 7-10 days, observing after hypha grows fully, and taking out to obtain mother strain.
Specifically, in the present embodiment, the concentration adjusting device includes a tray 21, a first electromagnet 22 provided at the top of the tray 21, and a second electromagnet 23 provided at the bottom of the tray 21; the tray 21 includes trays arranged at intervals up and down, and the trays are fixedly connected through connecting columns, so that a space for accommodating the culture container 3 is formed between the trays.
Before the sugar content of the culture solution is detected, a first magnetic field with constant size and periodically changing direction is generated between the first electromagnet 22 and the second electromagnet 23, the soft magnetic film layer 14 in the nutrition unit is magnetized and then drives the elastic rubber film to move up and down under the action of magnetic field force, and the culture solution is stirred, so that the culture solution forms convection, and the detection result error caused by the local concentration difference in the culture solution is avoided;
when the sugar degree value of the culture solution is adjusted, a second magnetic field with constant size and unchanged direction is generated between the first electromagnet 22 and the second electromagnet 23, the second magnetic field is a magnetic field in a pulse state, the magnetic field strength of the second magnetic field is larger than that of the first magnetic field, the magnetic field strength of the first magnetic field is insufficient to enable the upper elastic silicone rubber membrane 12 and the lower elastic silicone rubber membrane 12 to deform, after the soft magnetic membrane layer 14 is magnetized under the action of the second magnetic field, an adsorption effect is generated between the soft magnetic membrane layers 14 attached to the middle positions of the inner surfaces of the two elastic silicone rubber membranes 12 under the action of the magnetic field, so that the two elastic silicone rubber membranes 12 are inwards sunken, the volume in the accommodating cavity is reduced, the pressure is increased, the one-way valve structure of one-way liquid is opened, the volume in the accommodating cavity is increased, the pressure is reduced, the one-way valve structure of one-way liquid is opened under the self elastic action, the dispersion liquid outside the nutrition unit is sucked into the accommodating cavity, the nutrition block 13 is dissolved, the high concentration is alternately adjusted to the one-way liquid, the one-way liquid is consumed by the one-way liquid, and the medium is alternately released to the one-way liquid is in the state due to the fact that the high concentration is adjusted to the one-way liquid is in the one-way liquid, and the medium is in the state is released by the one-way liquid, and the medium is adjusted to have the high concentration effect, and the one-way liquid is consumed by the one-way liquid is discharged;
after the concentration adjustment is completed, a first magnetic field is generated between the first electromagnet 22 and the second electromagnet 23, the culture solution is subjected to convection stirring, and then sampling is performed to detect the sugar content of the culture solution, so that the first magnetic field and the second magnetic field are alternately generated between the first electromagnet 22 and the second electromagnet 23 until the sugar value of the culture solution reaches a required value.
According to the embodiment, the nutrient unit is introduced into the strain culture solution, the soft magnetic film layer 14 is utilized to be matched with the magnetic fields generated by the first electromagnet 22 and the second electromagnet 23, so that the quantitative supplement of the nutrient concentration of the strain culture solution and the stirring of the strain culture solution are realized, the pollution of the culture solution caused by introducing foreign bacteria into an external stirring tool and the additionally introduced culture solution in the culture process is avoided, the culture failure is avoided, and the success rate of the culture is improved.
The foregoing description is only one preferred embodiment of the invention, and therefore all changes and modifications that come within the meaning and range of equivalency of the structures, features and principles of the invention are intended to be embraced therein.
Claims (9)
1. The litchi fungus strain culture solution is characterized by comprising a dispersion solution and a nutrition unit; the nutrition unit comprises the following components in parts by weight: 15-20 parts of water, 4-7 parts of silicone rubber, 0.05-0.1 part of soft magnetic powder, 0.04-0.1 part of epoxy resin, 1-3 parts of carbomer 940, 6-8 parts of glucose, 2-3 parts of soybean meal, 1-3 parts of termite fungus garden and 0.3-0.4 part of magnesium sulfate;
the dispersion liquid comprises the following components in parts by weight: 0.05-0.1 part of DSA-5 defoamer, 0.1-0.15 part of monopotassium phosphate, 0.1-0.15 part of disodium hydrogen phosphate and the balance of water.
2. The litchi bacterial strain culture solution of claim 1, wherein the nutrition unit comprises a frame made of silicone rubber, elastic silicone rubber films are arranged on two opposite sides of the frame, the frame and the elastic silicone rubber films are enclosed to form a containing cavity, and a nutrition gel block is loaded in the containing cavity;
the inner surface of the elastic silicon rubber film is provided with a soft magnetic film layer consisting of soft magnetic powder, the peripheral wall of the frame body is provided with through holes, one elastic silicon rubber film is provided with elastic pieces at the periphery, two opposite elastic pieces are abutted against the inner side wall of the frame body and plug the corresponding through holes, the other two opposite elastic pieces are abutted against the outer side wall of the frame body and plug the corresponding through holes, and the elastic pieces and the through holes form a one-way valve structure; the outer surface of the elastic silicon rubber film is provided with a boss.
3. The litchi seed culture solution of claim 2, wherein the nutrient gel block is prepared by mixing carbomer, glucose, soybean meal, termite fungus garden and magnesium sulfate.
4. The litchi seed culture solution of claim 2, wherein a gap is provided between the elastic silicone rubber membrane and the nutrient gel block.
5. The litchi seed culture solution of claim 1, wherein the soft magnetic powder is iron powder or soft magnetic ferrite.
6. The preparation method of the litchi fungus strain culture solution is characterized by comprising the following steps:
s1, preparing a nutrition gel block by 6-8 parts by weight of glucose, 2-3 parts by weight of soybean meal, 1-3 parts by weight of termite fungus gardens, 0.3-0.4 part by weight of magnesium sulfate and 1-3 parts by weight of carbomer 940;
s2, preparing 4-7 parts by weight of silicon rubber into an elastic silicon rubber film and a frame, mixing 0.05-0.1 part by weight of soft magnetic powder with 0.02-0.05 part by weight of epoxy resin, and coating the mixture on the inner surface of the elastic silicon rubber film to form a soft magnetic film layer;
s3, embedding the nutrition gel block into the frame, and attaching an elastic silicon rubber film with a soft magnetic film layer to the two side walls of the frame through 0.02-0.05 part by weight of epoxy resin to obtain a nutrition unit;
s4, adding 1000ml of water into 0.05-0.1 part by weight of DSA-5 defoamer, 0.1-0.15 part by weight of monopotassium phosphate and 0.1-0.15 part by weight of disodium hydrogen phosphate to dissolve to form dispersion liquid, and controlling the pH value to be within the range of 6.5-7.5; after the completion, adding the nutrition units into the dispersion liquid, and fully stirring to form a suspension liquid;
s5, placing the suspension into a sterilizing pot, sterilizing for 45min at 120-125 ℃, and cooling to room temperature to obtain the litchi fungus strain culture solution.
7. The method for preparing a litchi bacterial strain culture solution as claimed in claim 6, wherein the preparation of the nutritional gel block comprises the following steps:
s11, respectively crushing termite fungus gardens, bean pulp and carbomer, and sieving the crushed termite fungus gardens, bean pulp and carbomer with a 100-mesh sieve;
s12, fully mixing glucose, soybean meal, termite fungus gardens and magnesium sulfate, adding 15-20 parts by weight of water after the mixing is completed, fully stirring to form a solution, putting the obtained solution into a sterilizing pot for sterilizing at 120-125 ℃ for 30min after the completion of the mixing, and cooling to obtain a nutrient solution;
s13, adding carbomer 940 into the nutrient solution, and fully stirring to form paste; after completion, the obtained paste is molded into blocks, and the nutrition gel blocks are obtained after drying.
8. The culture method of the litchi fungus strain is characterized by comprising the following steps:
a1, selecting robust fruiting bodies with the litchi bacteria maturity of 65-70%, and longitudinally cutting the fruiting bodies into fruiting body particles;
a2, adding the dispersion liquid into a culture container for storage, mixing and stirring the nutrition units and the fruiting body particles, separating the nutrition units after mixing, and adding the separated nutrition units into the culture container to finish inoculation of the mother seeds;
a3, placing the culture container in a concentration adjusting device, transferring the culture container into a constant temperature incubator for culturing for 1-2 days, observing whether contaminated bacteria are generated, and stopping culturing if the contaminated bacteria are generated;
otherwise, culturing for 3-4 days, observing whether fine granular hyphae are produced, and if so, detecting the sugar degree value of the culture solution;
if the sugar degree value is lower than the first preset value, adjusting the sugar degree value of the culture solution by a concentration adjusting device until the sugar degree value reaches the first preset value;
a4, culturing for 5-7 days, detecting whether the sugar degree value is lower than a second preset value, and if so, adjusting to the second preset value;
and A5, after culturing for 7 days, taking hypha, and then inoculating the hypha to a test tube culture medium, culturing for 7-10 days under the constant temperature condition, and then taking the hypha preferentially from the hypha to obtain the mother strain of the mother strain.
9. The culture method according to claim 8, wherein the concentration adjusting device comprises a tray, a first electromagnet arranged at the top of the tray, and a second electromagnet arranged at the bottom of the tray; the tray comprises tray bodies which are arranged at intervals up and down, and the tray bodies are fixedly connected through connecting columns, so that a space for accommodating the culture container is formed between the tray bodies.
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