CN117616129A - 用于产生莱苞迪苷d的组合物和方法 - Google Patents

用于产生莱苞迪苷d的组合物和方法 Download PDF

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Publication number
CN117616129A
CN117616129A CN202280024174.4A CN202280024174A CN117616129A CN 117616129 A CN117616129 A CN 117616129A CN 202280024174 A CN202280024174 A CN 202280024174A CN 117616129 A CN117616129 A CN 117616129A
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China
Prior art keywords
residue
polypeptide
rebaudioside
seq
b12gt
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Pending
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CN202280024174.4A
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English (en)
Chinese (zh)
Inventor
K·E·罗伯茨
A·赞格利宁
D·格拉布斯
N·D·克里斯蒂安森
J·J·哈夫拉奈克
Y-E·A·巴恩
A·德夫科塔
M·南斯
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ARZEDA CORP
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ARZEDA CORP
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Application filed by ARZEDA CORP filed Critical ARZEDA CORP
Publication of CN117616129A publication Critical patent/CN117616129A/zh
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1048Glycosyltransferases (2.4)
    • C12N9/1051Hexosyltransferases (2.4.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/44Preparation of O-glycosides, e.g. glucosides
    • C12P19/56Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1048Glycosyltransferases (2.4)
    • C12N9/1051Hexosyltransferases (2.4.1)
    • C12N9/1062Sucrose synthase (2.4.1.13)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/18Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y204/00Glycosyltransferases (2.4)
    • C12Y204/01Hexosyltransferases (2.4.1)
    • C12Y204/01013Sucrose synthase (2.4.1.13)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
CN202280024174.4A 2021-02-17 2022-02-17 用于产生莱苞迪苷d的组合物和方法 Pending CN117616129A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163150515P 2021-02-17 2021-02-17
US63/150,515 2021-02-17
PCT/US2022/016820 WO2022178145A1 (en) 2021-02-17 2022-02-17 Compositions and methods for producing rebaudioside d

Publications (1)

Publication Number Publication Date
CN117616129A true CN117616129A (zh) 2024-02-27

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ID=82931006

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202280024174.4A Pending CN117616129A (zh) 2021-02-17 2022-02-17 用于产生莱苞迪苷d的组合物和方法

Country Status (11)

Country Link
US (1) US20240368661A1 (https=)
EP (1) EP4294934A4 (https=)
JP (1) JP2024507361A (https=)
KR (1) KR20240010448A (https=)
CN (1) CN117616129A (https=)
BR (1) BR112023016512A2 (https=)
CA (1) CA3208720A1 (https=)
CO (1) CO2023010756A2 (https=)
MX (1) MX2023009628A (https=)
PE (1) PE20240694A1 (https=)
WO (1) WO2022178145A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN119799672A (zh) * 2025-01-15 2025-04-11 皖西学院 一种糖基转移酶突变体及其在合成红景天苷中的应用

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3266431A1 (en) * 2022-09-02 2024-03-07 Arzeda Corp. Compositions and methods for producing rebaudioside m
CN115947788B (zh) * 2022-10-12 2023-08-29 东北农业大学 色氨酸和亮氨酸跨链交互作用β-发卡抗菌肽WLF及制备方法和应用
EP4608984A2 (en) * 2022-10-26 2025-09-03 Arzeda Corp. Enzymatically mediated reactive crystallization of steviol glycosides
EP4724575A1 (en) * 2023-06-06 2026-04-15 Arzeda Corp. Compositions and methods for producing rebaudioside d and rebaudioside m
WO2026006475A1 (en) * 2024-06-25 2026-01-02 Arzeda Corp. Compositions and methods for glycosylation of steviol glycosides

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9752174B2 (en) * 2013-05-28 2017-09-05 Purecircle Sdn Bhd High-purity steviol glycosides
PE20200291A1 (es) * 2017-05-15 2020-02-05 Purecircle Usa Inc Glicosidos de esteviol de alta pureza
IL280302B2 (en) * 2018-07-30 2025-09-01 Codexis Inc Engineered glycosyltransferases and methods for glycosylation of steviol glycoside
EP3973069A4 (en) * 2019-05-23 2023-09-13 Arzeda Corp. COMPOSITIONS AND METHODS FOR PRODUCING STEVIOL GLYCOSIDES

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN119799672A (zh) * 2025-01-15 2025-04-11 皖西学院 一种糖基转移酶突变体及其在合成红景天苷中的应用

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Publication number Publication date
PE20240694A1 (es) 2024-04-10
EP4294934A4 (en) 2025-05-21
BR112023016512A2 (pt) 2023-10-24
JP2024507361A (ja) 2024-02-19
CA3208720A1 (en) 2022-08-25
EP4294934A1 (en) 2023-12-27
MX2023009628A (es) 2024-01-25
KR20240010448A (ko) 2024-01-23
US20240368661A1 (en) 2024-11-07
CO2023010756A2 (es) 2024-01-15
WO2022178145A1 (en) 2022-08-25

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