CN117256398A - Seedling hardening method for water coco seedlings - Google Patents

Abstract

The invention provides a seedling hardening method for water coco seedlings, which comprises the following steps: the water coco seedlings with the height of 20cm to 40cm are taken and placed under a shading net with the shading rate of 70 percent to 80 percent to be shaded for 28 days to 30 days, when the air temperature is higher than or equal to 26 ℃, shading is carried out, and when the air temperature is lower than 26 ℃, shading is not carried out. Aiming at the problem of low survival rate of transplanted seedlings in the existing water coconut seedling raising technology, the seedling hardening method increases the stress resistance of water coconut seedlings, so that the water coconut seedlings can survive in the living environment more advantageously; meanwhile, the seedling hardening method can reduce the closing time of the air holes, and is more beneficial to the accumulation of organic matters and the growth of the organic matters.

Description

A method for hardening water coconut seedlings

Technical field

The invention belongs to the technical field of water coconut seedling cultivation and relates to a seedling hardening method of water coconut seedlings.

Background technique

Water palm is a "viviparous" tropical coastal plant and an endangered species. Clusters of evergreen shrubs, 3 to 7 meters high. The water palm is distributed in some areas of Hainan. It is a typical tropical coastal plant and a relict plant. It is valuable for studying the phylogeny and origin of the palm family, tropical flora, paleontology, and paleogeography. In recent years, due to excessive cutting and fruit picking, the growth and natural distribution of water coconut forests have been greatly affected. If protection is not strengthened, it will become endangered. Therefore, there is an urgent need to develop an efficient water coconut propagation method to alleviate the endangered status of water coconut.

The existing water coconut seedling raising methods mainly focus on the collection of water coconut seeds and the germination of fruits into seedlings. The survival rate when the seedlings are moved to the water coconut habitat is not high. Therefore, if we want to alleviate the endangered status of water coconut, improving the survival rate of water coconut seedlings after they are transplanted from nurseries to habitats is a step that cannot be skipped.

Contents of the invention

The present invention aims to provide a seedling hardening method for water coconut seedlings, which increases the stress resistance of the water coconut seedlings and gives them more advantages in surviving in the habitat; at the same time, the stomata closing time of the leaves of the water coconut seedlings is reduced, and the water coconut seedlings are more resistant to stress. Conducive to the accumulation of organic matter and its own growth.

The technical solutions adopted by the present invention to achieve the above objects are:

A method for hardening water coconut seedlings, including: taking water coconut seedlings with a plant height of 20 to 40 cm and placing them under a shade net with a shading rate of 70% to 80% for 28 to 30 days. When the temperature Provide shading when the temperature is higher than or equal to 26°C, and do not provide shading when the temperature is lower than 26°C.

Preferably, the seedling hardening is carried out in an open-air nursery. A steel frame is built around the seedling hardening area, and a layer of shading net is laid on the steel frame.

More preferably, the seedling hardening area is 2-3m wide and 10-15m long.

More preferably, seedling bags are provided in the nursery.

More preferably, the seedling raising bag adopts a non-woven seedling raising bag with a cylinder diameter of 20-24cm and a depth of 20-24cm.

Preferably, it also includes selecting water coconut seedlings with a plant height of 90-120cm and a crown width of 80-100cm as qualified seedlings.

The beneficial effects of the present invention are:

Aiming at the problem of low transplant survival rate of seedlings in the existing water coconut seedling raising technology, the seedling hardening method of the present invention increases the stress resistance of water coconut seedlings, giving them more advantages in surviving in the habitat; because water coconuts are mainly distributed in In places such as Hainan, the weather is hot and the stomata of water coconut seedlings will be closed at noon, and the dark reaction of photosynthesis will be blocked, resulting in less accumulation of organic matter and slower growth. However, this method of seedling hardening will reduce the stomata closing time, which is more conducive to the removal of organic matter. Accumulation, own growth.

Description of the drawings

Figure 1 shows the detection results of superoxide dismutase (SOD) activity according to the embodiment of the present invention.

Figure 2 is the detection result of peroxidase (POD) activity according to the embodiment of the present invention.

Figure 3 is the detection result of catalase (CAT) activity according to the embodiment of the present invention.

Figure 4 is the detection result of soluble protein content (SP) in the embodiment of the present invention.

Detailed ways

In order to illustrate the present invention more clearly, the present invention will be further described in detail below with reference to the embodiments and the accompanying drawings. Those skilled in the art should understand that the content described below is illustrative rather than restrictive, and should not be used to limit the scope of the present invention.

Example

The experiment will be conducted from March 2022 to April 2022.

The experimental materials were taken from the Panggui Nanxia Village Nursery in the Dongzhai Port National Nature Reserve. The nursery was equipped with non-woven seedling bags with a cylindrical diameter of 24cm and a depth of 24cm. Seedling hardening is carried out in an open-air nursery. The seedling hardening area is 3m wide and 12m long. A steel frame is built around it and a layer of shading net is laid on the steel frame. Design five groups of shade nets with different shading rates, namely Group A (shading rate 60%), Group B (shading rate 70%), Group C (shading rate 80%), Group D (shading rate 90%) and CK There are five groups of water coconut seedlings with different shading rates, including group (shading rate 0). Each group has 30 pots of water coconut seedlings. The water coconut seedlings in groups A, B, C, and D are potted in greenhouses with sunshade nets. One plant per pot" style.

The specific operations of seedling hardening include: taking water coconut seedlings with a plant height of 20-40cm (the plant height of the same batch of seedlings will also be different, as long as they are within this range) and placing them under a shade net. The shading time is not fixed and is mainly affected by temperature. The annual average temperature of the water coconut's adapted distribution area is 23.8-25.5℃. When the temperature is higher than or equal to 26℃, the shade net is unfolded for shading. When the temperature is lower than 26℃, the shade net is folded. Shade nets do not provide shading.

The sampling dates for this experiment were March 16, March 23, March 30, April 7, and April 14. Six plants were randomly sampled from each group, and 10cm long fresh leaves were taken from each seedling. After sampling, put the fresh leaves into marked test tubes, then put them in an incubator and take them back to the laboratory for storage.

Superoxide dismutase (SOD) activity was measured according to the method of Giannopolitis & Ries (1977). 0.05 mol·L ^-1 pH 7.8 phosphate buffer was used as a blank instead of the enzyme solution to inhibit the photochemical reduction of nitroblue tetrazolium by 50%. Unit (U), enzyme activity is expressed in U·g ^-1 FW (where FW represents the fresh weight of the sample); peroxidase (POD) activity refers to the method of Chen Yizhu and Wang Yirou (1989), causing OD470 changes per minute 0.01 is 1 enzyme activity unit (U), and the enzyme activity is expressed in U·g ^-1 FW·min ^-1 ; the catalase (CAT) activity was measured according to the method of Chance & Maehly (1955), and the OD240 change was caused per minute. The amount of enzyme required for 0.01 is one enzyme activity unit (U), and the enzyme activity is expressed in U·g ^-1 FW·min ^-1 ; the soluble protein content was determined by Coomassie brilliant blue staining (Bradford, 1976). SP).

Referring to Figures 1 to 4, the results show that after 7, 14, 21, and 28 days of shading treatment, water coconut seedlings have the highest superoxide dismutase activity when they are under shading rates of 80%, 70%, 70%, and 80% respectively. The enzyme activity was 93.13%, 38.42%, 16.80%, and 56.12% higher than that of the shading rate group 0 respectively; the highest POD enzyme activity was at the shading rate of 80%, 70%, 70%, and 70%, respectively, which was higher than that of the shading rate group 0. The enzyme activity was 56.56%, 32.51%, 51.61%, and 52.34% higher; the highest CAT enzyme activity was under the shading rate of 80%, 70%, 70%, and 60%, respectively, which was 18.36%, 18.36%, and 18.36% higher than the enzyme activity in the shading rate group 0, respectively. 202.82%, 52.78%, 253.93%; the highest soluble protein content was at the shading rate of 80%, 70%, 70%, and 80% respectively, which were 25.43%, 20.49%, and 51.24% higher than the soluble protein content in the shading rate group 0. , 39.37%. Therefore, water coconut seedlings have higher stress resistance when the shading rate is between 70% and 80%.

Finally, select water coconut seedlings with a plant height of 90-120cm and a crown width of 80-100cm as qualified seedlings, and take them out of the nursery together with the seedling bags.

Obviously, the above-mentioned embodiments of the present invention are only examples to illustrate the present invention more clearly, and are not intended to limit the implementation of the present invention. For those of ordinary skill in the art, based on the above description, Other changes or modifications can be made in different forms, and all implementation methods cannot be exhaustively listed here. All obvious changes or modifications derived from the technical solution of the present invention are still within the protection scope of the present invention.

Claims (6)

1. A method of hardening water coconut seedlings comprising: the water coco seedlings with the height of 20cm to 40cm are taken and placed under a shading net with the shading rate of 70 percent to 80 percent to be shaded for 28 days to 30 days, when the air temperature is higher than or equal to 26 ℃, shading is carried out, and when the air temperature is lower than 26 ℃, shading is not carried out.

2. The seedling hardening method according to claim 1, wherein the seedling hardening is performed in an open nursery, a steel frame is built around the seedling hardening area, and a layer of shading net is laid on the steel frame.

3. The seedling hardening method according to claim 2, wherein the seedling hardening area is 2-3 m wide and 10-15 m long.

4. The seedling hardening method according to claim 2, wherein a seedling bag is provided in the nursery.

5. The seedling hardening method according to claim 4, wherein the seedling raising bag is a non-woven fabric seedling raising bag with a specification of 20-24 cm in diameter and 20-24 cm in depth.

6. The method of acclimatizing according to any one of claims 1 to 5, further comprising selecting a water coconut seedling having a plant height of 90 to 120cm and a crown width of 80 to 100cm as a qualified seedling.