CN117229983B - Nitrosogenic paraburkholderia PX-418, microbial inoculum and application thereof - Google Patents
Nitrosogenic paraburkholderia PX-418, microbial inoculum and application thereof Download PDFInfo
- Publication number
- CN117229983B CN117229983B CN202311507942.4A CN202311507942A CN117229983B CN 117229983 B CN117229983 B CN 117229983B CN 202311507942 A CN202311507942 A CN 202311507942A CN 117229983 B CN117229983 B CN 117229983B
- Authority
- CN
- China
- Prior art keywords
- paraburkholderia
- xenobiotic
- soil
- carrier
- microbial
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241001578292 Paraburkholderia Species 0.000 title claims abstract description 33
- 239000002068 microbial inoculum Substances 0.000 title claims abstract description 13
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 50
- 239000002676 xenobiotic agent Substances 0.000 claims abstract description 31
- 230000002034 xenobiotic effect Effects 0.000 claims abstract description 31
- 230000001580 bacterial effect Effects 0.000 claims abstract description 30
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 25
- 150000003071 polychlorinated biphenyls Chemical group 0.000 claims abstract description 24
- 241000196324 Embryophyta Species 0.000 claims abstract description 21
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 8
- 238000004321 preservation Methods 0.000 claims abstract description 8
- 229910019142 PO4 Inorganic materials 0.000 claims abstract description 4
- 238000009629 microbiological culture Methods 0.000 claims abstract description 4
- 239000010452 phosphate Substances 0.000 claims abstract description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims abstract description 4
- 238000004090 dissolution Methods 0.000 claims abstract description 3
- 239000001963 growth medium Substances 0.000 claims description 28
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 23
- 239000007788 liquid Substances 0.000 claims description 21
- 239000011574 phosphorus Substances 0.000 claims description 21
- 229910052698 phosphorus Inorganic materials 0.000 claims description 21
- 238000001035 drying Methods 0.000 claims description 16
- 238000012258 culturing Methods 0.000 claims description 14
- 239000007787 solid Substances 0.000 claims description 14
- 238000009630 liquid culture Methods 0.000 claims description 11
- 238000011282 treatment Methods 0.000 claims description 10
- 230000015556 catabolic process Effects 0.000 claims description 9
- 238000006731 degradation reaction Methods 0.000 claims description 9
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 claims description 8
- 239000001768 carboxy methyl cellulose Substances 0.000 claims description 8
- 238000001125 extrusion Methods 0.000 claims description 8
- 230000000813 microbial effect Effects 0.000 claims description 8
- 238000002360 preparation method Methods 0.000 claims description 8
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 claims description 8
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 claims description 8
- 229920001285 xanthan gum Polymers 0.000 claims description 8
- 238000002156 mixing Methods 0.000 claims description 7
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 claims description 6
- 230000008635 plant growth Effects 0.000 claims description 6
- -1 polychlorinated biphenyl PCB28 Chemical compound 0.000 claims description 6
- 239000000230 xanthan gum Substances 0.000 claims description 6
- 229940082509 xanthan gum Drugs 0.000 claims description 6
- 235000010493 xanthan gum Nutrition 0.000 claims description 6
- 230000001737 promoting effect Effects 0.000 claims description 5
- 229960000892 attapulgite Drugs 0.000 claims description 4
- 238000009835 boiling Methods 0.000 claims description 4
- 238000005469 granulation Methods 0.000 claims description 4
- 230000003179 granulation Effects 0.000 claims description 4
- 229910052625 palygorskite Inorganic materials 0.000 claims description 4
- 239000005995 Aluminium silicate Substances 0.000 claims description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 3
- 229920002472 Starch Polymers 0.000 claims description 3
- 240000008042 Zea mays Species 0.000 claims description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 3
- 235000012211 aluminium silicate Nutrition 0.000 claims description 3
- 235000005822 corn Nutrition 0.000 claims description 3
- 239000010903 husk Substances 0.000 claims description 3
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 239000008107 starch Substances 0.000 claims description 3
- 235000019698 starch Nutrition 0.000 claims description 3
- 239000004575 stone Substances 0.000 claims description 3
- GJCOSYZMQJWQCA-UHFFFAOYSA-N 9H-xanthene Chemical compound C1=CC=C2CC3=CC=CC=C3OC2=C1 GJCOSYZMQJWQCA-UHFFFAOYSA-N 0.000 claims description 2
- 240000004658 Medicago sativa Species 0.000 claims 1
- 239000002689 soil Substances 0.000 abstract description 38
- 239000008187 granular material Substances 0.000 abstract description 21
- 240000003768 Solanum lycopersicum Species 0.000 abstract description 12
- 230000012010 growth Effects 0.000 abstract description 12
- 239000003337 fertilizer Substances 0.000 abstract description 9
- 238000000034 method Methods 0.000 abstract description 6
- 244000005700 microbiome Species 0.000 abstract description 6
- 235000021049 nutrient content Nutrition 0.000 abstract description 6
- 230000004071 biological effect Effects 0.000 abstract description 3
- 230000021749 root development Effects 0.000 abstract description 3
- 230000007613 environmental effect Effects 0.000 abstract description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 11
- BZTYNSQSZHARAZ-UHFFFAOYSA-N 2,4-dichloro-1-(4-chlorophenyl)benzene Chemical compound C1=CC(Cl)=CC=C1C1=CC=C(Cl)C=C1Cl BZTYNSQSZHARAZ-UHFFFAOYSA-N 0.000 description 8
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 8
- 230000000694 effects Effects 0.000 description 8
- 239000002609 medium Substances 0.000 description 7
- 108090000623 proteins and genes Proteins 0.000 description 7
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 230000000593 degrading effect Effects 0.000 description 6
- 238000002791 soaking Methods 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 241000219823 Medicago Species 0.000 description 5
- 229910017053 inorganic salt Inorganic materials 0.000 description 5
- 238000000855 fermentation Methods 0.000 description 4
- 230000004151 fermentation Effects 0.000 description 4
- 238000012163 sequencing technique Methods 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 241000607757 Xenorhabdus Species 0.000 description 3
- 239000008367 deionised water Substances 0.000 description 3
- 229910021641 deionized water Inorganic materials 0.000 description 3
- 239000007789 gas Substances 0.000 description 3
- 108091008053 gene clusters Proteins 0.000 description 3
- 238000005286 illumination Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000002957 persistent organic pollutant Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 241000589151 Azotobacter Species 0.000 description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- 239000003708 ampul Substances 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000004178 biological nitrogen fixation Methods 0.000 description 2
- 239000001110 calcium chloride Substances 0.000 description 2
- 229910001628 calcium chloride Inorganic materials 0.000 description 2
- 239000001506 calcium phosphate Substances 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 description 2
- 229940061634 magnesium sulfate heptahydrate Drugs 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 2
- 235000019796 monopotassium phosphate Nutrition 0.000 description 2
- 239000002773 nucleotide Substances 0.000 description 2
- 125000003729 nucleotide group Chemical group 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 2
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 2
- 238000005067 remediation Methods 0.000 description 2
- 230000008439 repair process Effects 0.000 description 2
- 239000012266 salt solution Substances 0.000 description 2
- 239000008223 sterile water Substances 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 230000001502 supplementing effect Effects 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 229910021654 trace metal Inorganic materials 0.000 description 2
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 2
- 229940078499 tricalcium phosphate Drugs 0.000 description 2
- 229910000391 tricalcium phosphate Inorganic materials 0.000 description 2
- 235000019731 tricalcium phosphate Nutrition 0.000 description 2
- 108020004465 16S ribosomal RNA Proteins 0.000 description 1
- 108010071186 2,3-dihydroxybiphenyl oxygenase Proteins 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 241001453380 Burkholderia Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 238000007400 DNA extraction Methods 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
- 239000005977 Ethylene Substances 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 102000018997 Growth Hormone Human genes 0.000 description 1
- 108010051696 Growth Hormone Proteins 0.000 description 1
- 101100327510 Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) cfbD gene Proteins 0.000 description 1
- ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 description 1
- 101150062830 NIFK gene Proteins 0.000 description 1
- 102100030569 Nuclear receptor corepressor 2 Human genes 0.000 description 1
- 101710153660 Nuclear receptor corepressor 2 Proteins 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 240000000103 Potentilla erecta Species 0.000 description 1
- 235000016551 Potentilla erecta Nutrition 0.000 description 1
- 239000005708 Sodium hypochlorite Substances 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 239000002390 adhesive tape Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- HSFWRNGVRCDJHI-UHFFFAOYSA-N alpha-acetylene Natural products C#C HSFWRNGVRCDJHI-UHFFFAOYSA-N 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 231100000693 bioaccumulation Toxicity 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- PASHVRUKOFIRIK-UHFFFAOYSA-L calcium sulfate dihydrate Chemical compound O.O.[Ca+2].[O-]S([O-])(=O)=O PASHVRUKOFIRIK-UHFFFAOYSA-L 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 239000013064 chemical raw material Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 125000002534 ethynyl group Chemical group [H]C#C* 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 231100000086 high toxicity Toxicity 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- SURQXAFEQWPFPV-UHFFFAOYSA-L iron(2+) sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Fe+2].[O-]S([O-])(=O)=O SURQXAFEQWPFPV-UHFFFAOYSA-L 0.000 description 1
- 101150044508 key gene Proteins 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 125000005647 linker group Chemical group 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 229940099596 manganese sulfate Drugs 0.000 description 1
- 239000011702 manganese sulphate Substances 0.000 description 1
- 235000007079 manganese sulphate Nutrition 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 229910052750 molybdenum Inorganic materials 0.000 description 1
- 239000011733 molybdenum Substances 0.000 description 1
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical class CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 1
- 101150101270 nifD gene Proteins 0.000 description 1
- 101150009446 nifN gene Proteins 0.000 description 1
- 239000000618 nitrogen fertilizer Substances 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000002096 quantum dot Substances 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 description 1
- 235000010234 sodium benzoate Nutrition 0.000 description 1
- 239000004299 sodium benzoate Substances 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 239000007160 ty medium Substances 0.000 description 1
- 238000012070 whole genome sequencing analysis Methods 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to the technical field of environmental microorganisms, in particular to xenobiotic paraburkholderia PX-418, a microbial inoculum and application thereof. Xenobiotic-eating paraburkholderiaParaburkholderia xenovorans) PX-418 is preserved in 2023, 9 and 11 days to China general microbiological culture Collection center with the preservation number of CGMCC No.28418. The strain can efficiently degrade polychlorinated biphenyl, has excellent autonomous nitrogen fixation capacity and phosphate dissolution capacity, and can remarkably promote the growth and root development of tomato plants. The PX-418 granules prepared by the method are used as biological bacterial fertilizer, so that the soil is obviously improved, and the biological activity and the available nutrient content of the soil are improved. The microbial inoculum is used as a bioremediation agent to be combined with plants, and plays an important role in bioremediation of polychlorinated biphenyl polluted soil.
Description
Technical Field
The invention relates to the technical field of environmental microorganisms, in particular to xenobiotic paraburkholderia PX-418, a microbial inoculum and application thereof.
Background
Biological nitrogen fixation refers to that nitrogen fixation microorganisms in nature catalyze N in the air under certain specific conditions and under the catalysis of intracellular azotase 2 Reduction to NH 3 Is a main source of ammonia in natural ecosystem, plays an important role in the circulation process of geochemical nitrogen, and is also an important source of nitrogen required by agricultural, forest and grass industries. Nitrogen and phosphorus are mineral nutrients which are necessary for normal growth of plants, some leguminous plants in nature can provide effective nitrogen sources for themselves through combined nitrogen fixation, and most plants do not have autonomous nitrogen fixation or combined nitrogen fixation capability; the level of available phosphorus in the soil is one of the key factors influencing plant growth, however, 95% of indissolvable or fixed form of ineffective phosphorus in the soil is difficult to directly absorb and utilize, so that a large amount of cultivated land soil has the phenomenon of phosphorus deficiency.
The modern agriculture solves a great deal of demands of nitrogen and phosphorus in the production process of crops by applying fertilizers in a large area, but the problems of soil hardening, salinization, water eutrophication and other environmental pollution are easily caused due to the application of a great deal of unreasonable nitrogenous fertilizers and phosphate fertilizers in the production process for a long time. The microbial fertilizer with the functions of fixing nitrogen and dissolving phosphorus is used, the huge potential of biological nitrogen fixation is exerted, the ineffective phosphorus form in soil is converted into the effective phosphorus form which can be absorbed by plants, and nitrogen sources and phosphorus sources are provided for plant production, so that the use amount of chemical nitrogen fertilizer and phosphorus fertilizer is reduced, the green and sustainable development of modern agriculture is realized, and the microbial fertilizer has huge application and development prospects.
Polychlorinated biphenyl is an important chemical raw material for artificial synthesis, has the characteristics of high toxicity, lasting environment, bioaccumulation, semi-volatilization, long-distance migration and the like, is a typical Persistent Organic Pollutant (POPs), is one of 12 main POPs which are widely concerned internationally, and lacks an effective degradation system in nature, so that the matter can stay in the environment for a long time. Screening degradation bacteria capable of degrading polychlorinated biphenyl for bioremediation is an important means for controlling and treating polychlorinated biphenyl pollution. In addition, the plant has positive effect on the repair of polychlorinated biphenyl pollution, and the plant can accumulate and metabolize polychlorinated biphenyl, but the metabolism rate is slower. In recent years, the combined action of plants and rhizosphere microorganisms for degrading polychlorinated biphenyl becomes a research hot spot, and the combined restoration of polychlorinated biphenyl polluted soil by adopting polychlorinated biphenyl degrading microorganisms with plant growth promoting effect and plants has a huge application prospect.
Disclosure of Invention
In order to develop the effect of polychlorinated biphenyl degrading microorganisms and plants in combined soil restoration, the invention provides a xenobiotic paraburkholderia PX-418, a microbial inoculum and application thereof.
In a first aspect, the invention provides a strain of paraburkholderia PX-418 for fixing nitrogen and dissolving phosphorus, which is paraburkholderiaParaburkholderia xenovorans) PX-418 is preserved in 2023, 9 and 11 days to China general microbiological culture Collection center with the preservation number of CGMCC No.28418 and the preservation address of North Chen Xiu Lu No. 1 and 3 of the Chaoyang district of Beijing city.
In a second aspect, the present invention provides an application of the xenobiotic paraburkholderia PX-418 in preparing a product for degrading polychlorinated biphenyl PCB28 by combining plants.
Further, the product of the polychlorinated biphenyl PCB28 is a product for fixing nitrogen and dissolving phosphorus and promoting plant growth.
In a third aspect, the invention also provides a xenobiotic paraburkholderia PX-418 microbial inoculum, which comprises xenobiotic paraburkholderia PX-418 microbial inoculum, sodium carboxymethyl cellulose, xanthan gum and a carrier.
Further, the mass ratio of the PX-418 bacterial liquid to the carrier is 1:25, sodium carboxymethylcellulose accounts for 5% of the total mass of the PX-418 bacterial liquid and the carrier, and xanthan accounts for 0.5% of the total mass of the PX-418 bacterial liquid and the carrier.
Further, the carrier is one or more of diatomite, medical stone, kaolin, attapulgite, starch and corn husk powder.
Further, the preparation method comprises the following steps:
(1) Selecting PX-418 single colonies on a TY solid culture medium, inoculating the single colonies to a TY liquid culture medium, culturing overnight at 32 ℃, inoculating the culture solution into the TY liquid culture medium according to the volume percentage of 5% -10%, and culturing for 24-48 h at 32 ℃ and 150-200 rpm to obtain PX-418 bacterial solution;
(2) And mixing the PX-418 bacterial liquid with a carrier, adding sodium carboxymethylcellulose and xanthan gum, fully mixing, adding into an extrusion granulator for extrusion granulation, and drying at 35-40 ℃ to obtain the PX-418 bacterial agent.
Further, the drying treatment adopts a fluidized bed for boiling drying, and the moisture content is less than or equal to 5 percent.
The invention has the beneficial effects that:
the xenobiotic paraburkholderia PX-418 provided by the invention can efficiently degrade polychlorinated biphenyl, has excellent autonomous nitrogen fixation capacity and phosphate dissolution capacity, and can remarkably promote the growth and root development of tomato plants; the PX-418 granules prepared by the method can be used as biological bacterial fertilizer, and can be used for obviously improving soil and increasing the biological activity and the available nutrient content of the soil. The microbial inoculum can also be used as a bioremediation agent to be combined with plants, and plays an important role in bioremediation of polychlorinated biphenyl polluted soil.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required to be used in the description of the embodiments or the prior art will be briefly described below, and it will be obvious to those skilled in the art that other drawings can be obtained from these drawings without inventive effort.
FIG. 1 is a photograph of pure culture of the xenobiotic paraburkholderia PX-418 strain in TY solid medium.
FIG. 2 is a heat map relating to the strain ANI of xenobiotic-fed Paraquatichalia PX-418.
FIG. 3 is a heat map of the strain AAI of xenobiotic-fed Burkholderia paraibori PX-418.
FIG. 4 is a nitrogen fixation related gene cluster of xenorhabdus paraburkholderia strain PX-418.
FIG. 5 is a graph showing the characteristics of the xenobiotic paraburkholderia strain PX-418; wherein A is the growth condition of the strain on an Ababetes culture medium, and B is the growth condition of the strain on an inorganic phosphorus solid culture medium.
FIG. 6 is the degradation efficiency of xenorhabdus paraburkholderia strain PX-418 in the inorganic salt medium of high concentration PCB 28.
Detailed Description
In order to make the technical solution of the present invention better understood by those skilled in the art, the technical solution of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present invention without making any inventive effort, shall fall within the scope of the present invention.
The following examples were prepared using the following Abbe's medium:
10g of glucose, 0.2g of monopotassium phosphate, 0.2g of magnesium sulfate heptahydrate, 0.2g of sodium chloride, 5g of calcium carbonate, 0.1g of calcium sulfate dihydrate and 18g of agar are mixed, and water is added to 1000mL; adjusting the pH to 6.8-7.0, and sterilizing for 20min at 121 ℃.
The TY medium used in the following examples was prepared as follows:
1g of yeast extract, 10g of tryptone and 0.2g of calcium chloride are mixed, 1L of deionized water is supplemented, and the pH is adjusted to 7.0.
The preparation method of the inorganic phosphorus solid medium used in the following examples is as follows:
preparation of inorganic phosphorus solid medium: 5g of tricalcium phosphate, 10g of glucose, 0.5g of ammonium sulfate, 0.3g of sodium chloride, 0.3g of magnesium sulfate heptahydrate, 0.3g of potassium chloride, 0.03g of manganese sulfate and 0.03g of ferrous sulfate heptahydrate are dissolved in distilled water, 1000mL of distilled water is used for supplementing, pH 7.0-7.2 is used, and sterilization is carried out for 20min at 121 ℃.
EXAMPLE 1 isolation and identification of strains
1. Isolation and purification of strains
(1) Bacterial strain source: and collecting yellow river beach soil in eastern ying city of Shandong province in 5 months 2022.
(2) Domestication culture:
(1) preparing a liquid inorganic salt culture medium: 3g of monopotassium phosphate, 2g of disodium hydrogen phosphate, 0.15g of magnesium sulfate, 0.2g of calcium chloride, 0.15g of sodium chloride, 1.0g of ammonium sulfate, 0.5mL of trace metal salt solution, 15mg of polychlorinated biphenyl, and the deionized water is used for supplementing 1L, and the pH value is adjusted to 7.0; wherein the polychlorinated biphenyl is 2, 4-trichlorobiphenyl (PCB 28), and each liter of trace metal salt solution contains EDTA 5g and CaCO 3 10g、FeSO 4 •7H 2 O 5g、MnSO 4 •H 2 O 10g、ZnSO 4 •7H 2 O 1g、Na 2 B 4 O 7 •10H 2 O 17.7mg、CuSO 4 •5H 2 39.2mg of O and the balance of deionized water.
(2) 5g of the soil sample was weighed and added to the above liquid inorganic salt medium, followed by shaking culture at 30℃and 180rpm for 7 days.
(3) Separating and purifying: taking 100 mu L of domesticated culture medium, continuously and gradiently diluting and uniformly coating the domesticated culture medium on an Ababetes culture medium, and separating to obtain free-living nitrogen-fixing bacteria; selecting strains with different forms, and continuing purifying and culturing in the liquid inorganic salt culture medium prepared in the step (2) to obtain a single strain.
The strain PX-418 grows fast on TY solid medium, the culture temperature is 32 ℃, clear colonies can be formed after 24 hours, and the colonies are round, white to light brown, smooth in surface and convex (shown in figure 1).
2. Strain identification and nitrogen fixation gene prediction
(1) Sequencing experiment procedure
Extracting genome DNA of strain PX-418 with bacterial genome DNA extraction kit (available from Tiangen Biochemical technology (Beijing)) and detecting purity and integrity of DNA by agarose gel electrophoresis, randomly breaking the detected DNA sample into fragments with length of about 350bp by Covaris ultrasonic breaker, and using NEBNEext ® Ultra TM DNA Library Prep Kit for Illumina kit (from New England Biolabs company, U.S.) completed the entire library preparation by end repair, tailing, sequencing linker addition, purification, PCR amplification, and the like. Quantifying by using Qubit, and performing platform sequencing on Illumina NovaSeq PE after detection is qualified; by SMRTbell TM Template Prep Kit kit (from Pacific BioSciences (PacBIO) A10K SMRT Bell library was constructed and sequenced with the PacBIO platform after detection was passed.
(2) Biological information analysis flow
After sequencing is completed, low-quality data is removed from the original data, the data is assembled by adopting Unicycler software, and GC content statistics and evaluation of other species pollution sequences and contig length evaluation are carried out by using quick software.
Gene prediction: annotation information such as CDS, gene, tRNA of the genome was obtained by annotating the genome sequence generated by Unicycler with a Prokka annotation tool.
And (3) strain identification: all 16S sequence results predicted using Prokka were aligned (blastn) to the SILVA-16S database to obtain results, and the average nucleotide similarity ANI and amino acid identity AAI were calculated, as shown in fig. 2, 3.
The results showed that strain PX-418 andParaburkholderia xenovoransLB400 is in the same branch, and its 16S rRNA gene similarity is 100%, andParaburkholderia xenovorans4B gene similarity of 98.91%, withParaburkholderia xenovoransThe average nucleotide similarity (ANI) and Amino Acid Identity (AAI) of the 4B strain were 98.96% and 99.17%, respectively. Thus, the strain PX-418 was identified as xenorhabdus paraburkholderiaParaburkholderia xenovorans) Named as xenobiotic paraburkholderia PX-418.
Nitrogen fixation gene prediction: using the bacterial genome function annotation tool Prokka, a nitrogen fixation-related gene cluster was found by genetic prediction and function annotation of the Unicycler generated asembly. Fasta genomic sequence file (FIG. 4).
After whole genome sequencing and assembly, the presence of a nitrogen-fixing gene cluster in the genome of strain PX-418 was found by annotation by Prokka, comprisingnifH、nifD、nifK、nifE、nifN andnifb and other conserved genes; found that 2, 3-dihydroxybiphenyl-1, 2-dioxygenase gene exists in the genome of the strain PX-418bphC) Is a key gene for degrading polychlorinated biphenyl.
3. Preservation of strains
The xenobiotic paraburkholderia PX-418 is sent to the China general microbiological culture Collection center with the following preservation information:
classification naming: xenobiotic paraburkholderiaParaburkholderia xenovoransDeposit number: CGMCC No.28418, date of preservation: 2023, 9, 11, deposit address: no. 1 and No. 3 of the north cinquefoil of the morning sun area of beijing city.
Example 2 determination of degradation of polychlorinated biphenyls by xenobiotic paraburkholderia PX-418
Inoculating 10% of PX-418 fermentation broth in logarithmic growth phase into liquid inorganic salt culture medium containing PCB28 with initial concentration of 25mg/L, shaking culturing at 32deg.C at 180 r/min, collecting fermentation broth at 24h, 48h, 72h, 96h, 120h, 144h, 168h, extracting with dehydrated n-hexane for 3 times, rotary evaporating the extract to 2mL, detecting residual amount of PCB28 by gas chromatograph, calculating degradation rate of PX-418 to PCB28, and repeating for 3 times. As a result, as shown in FIG. 6, it was found that the degradation rate of the strain PX-418 to the polychlorinated biphenyl PCB28 was 85% within 7 days under the condition that the initial concentration of polychlorinated biphenyl was 25 mg/L.
Example 3 determination of the somatotropin of xenobiotic paraburkholderia PX-418
Diluting and uniformly coating the separated and purified PX-418 strain on an Abbe's disease culture medium for culture, wherein the growth condition is shown as figure 5A, the PX-418 strain grows slowly on the Abbe's disease culture medium, clear colonies can be formed in 48 hours, and sticky transparent colonies are formed; the PX-418 after separation and purification is diluted and evenly coated on an inorganic phosphorus solid culture medium, the growth condition is shown in figure 5B, and a transparent ring of phosphorus dissolving of bacterial colony is visible.
(1) Determination of Nitrogen fixation enzyme Activity
Inoculating the PX-418 strain into TY liquid culture medium, culturing for 24 hr, centrifuging to collect thallus, and re-suspending thallus with Abbe's liquid culture medium to obtain 10 8 CFU/mL azotobacter suspension. Transferring 100 mu L of azotobacter suspension into an ampoule filled with 2mL of Ababeta-base liquid culture medium, and culturing at 32 ℃ for 24 hours; 1mL of air is extracted from the ampoule by a disposable syringe, 1mL of acetylene is injected, and the needle eye is sealed by adhesive tape; the culture was continued for 24 hours, 100. Mu.L of a gas sample was withdrawn, and the ethylene peak was measured on a gas chromatograph, and the activity of the nitrogen fixation enzyme was calculated. As a result, the activity of the nitrogen-fixing enzyme of the PX-418 strain was 22.43 nmol/(mL.h).
(2) Determination of phosphorus-dissolving Capacity
(1) PX-418 single colony on the TY solid plate is picked up and connected to the inorganic phosphorus solid culture medium, and is cultured for 48 hours; the cross-over method measures the diameter of the colony phosphorus-dissolving transparent ring and the diameter of the colony, and calculates the ratio of the diameter of the colony phosphorus-dissolving transparent ring to the diameter of the colony to be 3.68.
(2) PX-418 single colony on TY solid plate is picked up and connected to a 250mL triangular flask containing 50mL inorganic phosphorus solid culture medium, no bacteria are used as blank control, after culturing for 72h at 180rpm and 32 ℃, the culture solution is filled into a centrifuge tube, and the centrifuge tube is centrifuged for 10min at 4 ℃ and 10000 g. Taking supernatant, and measuring the content of available phosphorus by a molybdenum blue colorimetric method, wherein after the supernatant is cultured for 72 hours at the temperature of 32 ℃, the conversion amount of PX-418 to tricalcium phosphate reaches 28.34mg/L.
Example 4 growth promoting effect of xenobiotic-fed Paraquaticus PX-418 on tomato seedlings
Tomato seeds were sterilized with 5% sodium hypochlorite and 75% alcohol for 5min, respectively, and washed with sterile water 3 times for 30s each. Then placing the seeds in a flat plate with sterile water-soaked filter paper laid at the bottom, placing the flat plate in an illumination incubator, and controlling illumination to be 16h illumination/8 h dark period, the temperature to be 22+/-2 ℃ and the relative humidity to be 60% -70%. After the seeds germinate to 1-2 cm, soaking the seeds in 1X 10 5 CFU/mL、1×10 6 CFU/mL and 1X 10 7 In CFU/mL PX-418 bacterial liquid, taking TY culture medium without PX-418 bacterial strain as a control, soaking for 1h, sowing the soaked seeds in a nutrition pot with a nutrition matrix, treating 25 strains per pot at each concentration, repeating for 3 times, and placing in a light incubator at 25 ℃ for culturing. Sampling after 35 days, carefully cleaning root soil, and counting plant height, root length and fresh weight, and the results are shown in Table 1.
TABLE 1 Effect of xenobiotic paraburkholderia PX-418 on tomato seedling growth
Note that: the difference between the treatments is obvious in the same column of different lower case lettersP<0.05）。
As can be seen from the data in Table 1, a concentration of 1X 10 was used 7 The CFU/mL PX-418 bacterial liquid is used for soaking tomato seeds for 1h, so that the root length, plant height, fresh weight and dry weight of tomato seedlings can be obviously increased, and the growth of the tomato seedlings can be effectively promoted by soaking the seed with xenobiotic paraburkholderia PX-418.
Example 5 preparation of xenobiotic paraburkholderia PX-418 granules
(1) And (3) picking PX-418 single colony on the TY solid plate to TY liquid culture medium, culturing at the temperature of 32 ℃ overnight, and then inoculating the bacterial liquid with the volume percentage of 5% into a 500mL triangular flask containing 100mL TY liquid culture medium, and culturing for 48h at the temperature of 32 ℃ and the rotating speed of 150 rpm.
(2) After fermentation, mixing PX-418 bacterial liquid with a carrier (comprising diatomite, medical stone and attapulgite) according to a mass percentage of 1:15, adding 1% of sodium carboxymethyl cellulose, 0.2% of xanthan gum and 0.1% of sodium benzoate into the carrier for full mixing, adding into an extrusion granulator for extrusion granulation, adopting a boiling drying fluidized bed for low-temperature drying treatment, wherein the drying temperature is 35-40 ℃, and drying until the water content is below 5%, thus obtaining the PX-418 granules.
Example 6 preparation of xenobiotic paraburkholderia PX-418 granules
(1) And (3) picking PX-418 single colony on the TY solid plate to TY liquid culture medium, culturing at 32 ℃ overnight, and then inoculating 10% of bacterial liquid in a 500mL triangular flask containing 100mL TY liquid culture medium, and culturing for 24h at 32 ℃ and 200 rpm.
(2) After fermentation, mixing PX-418 bacterial liquid with a carrier (comprising kaolin, attapulgite, starch and corn husk powder) according to a mass percentage of 1:25, adding 5% sodium carboxymethyl cellulose and 0.5% xanthan gum to fully mix the bacterial liquid and the carrier, adding the mixture into an extrusion granulator for extrusion granulation, adopting a boiling drying fluidized bed for low-temperature drying treatment, and drying at a drying temperature of 35-40 ℃ until the water content is below 5%, thus obtaining the PX-418 granules.
Example 7 Effect of xenobiotic feeding Paraquaporia PX-418 granules on soil nutrient content
(1) After the seeds germinate to 1-2 cm, soaking the seeds in 1X 10 7 In CFU/mL PX-418 bacterial liquid, a TY culture medium without the PX-418 bacterial strain is used as a control, and after soaking for 1h, the soaked seeds are reserved.
(2) The PX-418 granule prepared in example 5 was added to natural soil (2.5 g/kg), put into pots, 3kg per pot, and planted into treated tomato seeds as PX-418 group. The PX-418 bacterial liquid was replaced with sterile water, and granules prepared according to step (2) of example 5 were added to natural soil (2.5 g/kg), and placed in pots, 3kg each, and the treated tomato seeds were inoculated as a control group. Each group was provided with 10 pots. After 100 days of growth, soil was collected for analysis of soil nutrient content, and the results are shown in table 2.
TABLE 2 Effect of seeding PX-418 granules on soil nutrient content
Note that: the difference between the treatments is obvious in the same column of different lower case lettersP<0.05）。
As can be seen from Table 2, the organic matter, total nitrogen, available phosphorus and quick-acting potassium content of the soil inoculated with the PX-418 granule were significantly increased as compared with the control group. Then the bacterial and fungal contents in the soil are detected by fluorescent quantitative PCR, and the copy number of fungus ITS in each gram of soil of PX-418 group and control group is 1.48 multiplied by 10 9 And 8.2X10 8 Bacterial 16s copy numbers were 5.62X10 respectively 9 And 4.37X10 9 It was demonstrated that inoculation of PX-418 granules increased the microbial content in the soil.
Example 8 xenobiotic paraburkholderia PX-418 granules and plant combined remediation of polychlorinated biphenyl contaminated soil
(1) Preparing polychlorinated biphenyl PCB28 polluted soil: dissolving the PCB28 for test in acetone, adding a proper amount of the acetone into natural soil, adjusting the concentration to about 1000 mug/kg, fully and uniformly stirring, oscillating on an oscillator for about one week, placing for 7 days to fully volatilize the acetone, sub-packaging the polychlorinated biphenyl PCB28 polluted soil into pots before planting, and adding tap water into 3kg of each pot for balancing for standby.
(2) The test treatments were respectively: blank (CK), alfalfa (P, 10 alfalfa seeds per pot), PX-418 granules (B, PX-418 granule inoculum size 2.5 g/kg) were planted, alfalfa (P+B), and PX-418 granules (P+B, 10 alfalfa seeds per pot, PX-418 granule inoculum size 2.5 g/kg) were inoculated. Each treatment group was repeated 4 times. After 90 days of growth, the plant samples and rhizosphere soil samples were carefully collected. Gently shaking to obtain rhizosphere soil, naturally air-drying at room temperature, sieving with 100 mesh sieve, and measuring PCB28 content in soil.
TABLE 3 PCB28 content in rhizosphere soil under different treatments
Note that: the different letters in the same column represent obvious differenceP<0.05）。
As can be seen from Table 3, the combined remediation of PX-418 granules with plants increased the effect of degradation of polychlorinated biphenyls in the soil as compared to the alfalfa treatment groups alone and the PX-418 granules alone, which may be related to the increased absorption and accumulation of PCBs by the PX-418 granules promoting plant growth.
In conclusion, the xenobiotic paraburkholderia PX-418 provided by the invention can degrade polychlorinated biphenyl, has excellent autonomous nitrogen fixation capacity and phosphorus dissolving capacity, and can obviously promote the growth and root development of tomato plants; the PX-418 granules prepared by the method can also be used as biological bacterial fertilizer, promote the increase of nutrients in tomato plants, obviously improve soil and improve the biological activity and available nutrient content of the soil. The microbial inoculum can also be used as a bioremediation agent to be combined with plants, and has great application value in the bioremediation of polychlorinated biphenyl polluted soil.
Although the present invention has been described in detail by way of preferred embodiments with reference to the accompanying drawings, the present invention is not limited thereto. Various equivalent modifications and substitutions may be made in the embodiments of the present invention by those skilled in the art without departing from the spirit and scope of the present invention, and it is intended that all such modifications and substitutions be within the scope of the present invention/be within the scope of the present invention as defined by the appended claims.
Claims (8)
1. A strain of paraburkholderia PX-418 for fixing nitrogen and dissolving phosphorus is characterized in that paraburkholderia parasea isParaburkholderia xenovorans) PX-418 is preserved in 2023, 9 and 11 days to China general microbiological culture Collection center with the preservation number of CGMCC No.28418 and the preservation address of North Chen Xiu Lu No. 1 and 3 of the Chaoyang district of Beijing city.
2. Use of xenobiotic paraburkholderia PX-418 according to claim 1 for the preparation of a product for the degradation of polychlorinated biphenyl PCB28 in combination with a plant, wherein the plant is alfalfa.
3. The use according to claim 2, wherein the product of the combined plant degradation of polychlorinated biphenyl is a nitrogen fixation and phosphate dissolution product for promoting plant growth.
4. The xenobiotic paraburkholderia PX-418 microbial inoculum is characterized by comprising xenobiotic paraburkholderia PX-418 microbial inoculum, sodium carboxymethyl cellulose, xanthan gum and a carrier.
5. The xenobiotic paraburkholderia PX-418 microbial agent according to claim 4, wherein the mass ratio of the PX-418 microbial liquid to the carrier is 1:25, the sodium carboxymethyl cellulose accounts for 5% of the total mass of the PX-418 microbial liquid and the carrier, and the xanthan accounts for 0.5% of the total mass of the PX-418 microbial liquid and the carrier.
6. The xenobiotic paraburkholderia PX-418 microbial agent according to claim 4, wherein the carrier is one or more of diatomite, medical stone, kaolin, attapulgite, starch and corn husk powder.
7. The xenobiotic paraburkholderia PX-418 bacterial agent according to claim 4, wherein the preparation method comprises the following steps:
(1) Selecting PX-418 single colonies on a TY solid culture medium, inoculating the single colonies to a TY liquid culture medium, culturing overnight at 32 ℃, inoculating the culture solution into the TY liquid culture medium according to the volume percentage of 5% -10%, and culturing for 24-48 h at 32 ℃ and 150-200 rpm to obtain PX-418 bacterial solution;
(2) And mixing the PX-418 bacterial liquid with a carrier, adding sodium carboxymethylcellulose and xanthan gum, fully mixing, adding into an extrusion granulator for extrusion granulation, and performing low-temperature drying treatment to obtain the PX-418 bacterial agent.
8. The xenobiotic paraburkholderia PX-418 microbial inoculum according to claim 7, which is characterized in that a fluidized bed for boiling drying is adopted for low-temperature drying, the drying temperature is 35-40 ℃, and the drying is carried out until the moisture content is less than or equal to 5%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311507942.4A CN117229983B (en) | 2023-11-14 | 2023-11-14 | Nitrosogenic paraburkholderia PX-418, microbial inoculum and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311507942.4A CN117229983B (en) | 2023-11-14 | 2023-11-14 | Nitrosogenic paraburkholderia PX-418, microbial inoculum and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN117229983A CN117229983A (en) | 2023-12-15 |
| CN117229983B true CN117229983B (en) | 2024-01-30 |
Family
ID=89096962
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311507942.4A Active CN117229983B (en) | 2023-11-14 | 2023-11-14 | Nitrosogenic paraburkholderia PX-418, microbial inoculum and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117229983B (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110669686A (en) * | 2019-03-07 | 2020-01-10 | 慕恩（广州）生物科技有限公司 | Burkholderia and application thereof |
| WO2020011052A1 (en) * | 2018-07-09 | 2020-01-16 | 山东省科学院生态研究所 | Microbacterium paraoxydans and preparation method for broad-spectrum polychlorinated biphenyl enzyyme preparation thereof and use thereof |
| CN113362890A (en) * | 2021-04-28 | 2021-09-07 | 中国科学院生态环境研究中心 | Method for evaluating organic matter degradation potential of biological filter material |
| CN116064307A (en) * | 2022-10-12 | 2023-05-05 | 中南林业科技大学 | Burkholderia strain capable of efficiently dissolving phosphorus and application thereof |
| CN116751709A (en) * | 2023-05-31 | 2023-09-15 | 浙江省林业科学研究院 | Burkholderia strain B2OP and application thereof |
| CN116987621A (en) * | 2023-05-24 | 2023-11-03 | 中国科学院沈阳应用生态研究所 | Burkholderia sp and application thereof |
-
2023
- 2023-11-14 CN CN202311507942.4A patent/CN117229983B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020011052A1 (en) * | 2018-07-09 | 2020-01-16 | 山东省科学院生态研究所 | Microbacterium paraoxydans and preparation method for broad-spectrum polychlorinated biphenyl enzyyme preparation thereof and use thereof |
| CN110669686A (en) * | 2019-03-07 | 2020-01-10 | 慕恩（广州）生物科技有限公司 | Burkholderia and application thereof |
| CN113362890A (en) * | 2021-04-28 | 2021-09-07 | 中国科学院生态环境研究中心 | Method for evaluating organic matter degradation potential of biological filter material |
| CN116064307A (en) * | 2022-10-12 | 2023-05-05 | 中南林业科技大学 | Burkholderia strain capable of efficiently dissolving phosphorus and application thereof |
| CN116987621A (en) * | 2023-05-24 | 2023-11-03 | 中国科学院沈阳应用生态研究所 | Burkholderia sp and application thereof |
| CN116751709A (en) * | 2023-05-31 | 2023-09-15 | 浙江省林业科学研究院 | Burkholderia strain B2OP and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| Decontamination of a polychlorinated biphenylscontaminated soil by phytoremediation-assisted bioaugmentation;C. Secher et.al;Biodegradation(第24期);549-562 * |
| Degradation of highly chlorinated PCBs by Pseudomonas strain LB400;Lawrence H. Bopp;Journal of Industrial Microbiology;23-29 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN117229983A (en) | 2023-12-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111849815B (en) | Plant growth promoting rhizosphere strain Gxun-20 and application thereof in plant growth promotion | |
| CN101244955B (en) | Biological fertilizer and manufacture method thereof | |
| CN109679870B (en) | A kind of biological organic fertilizer and preparation method thereof with water conservation drought resisting function | |
| CN109868242B (en) | Salt-tolerant acetoin-producing bacillus subtilis and application thereof | |
| CN110577911A (en) | bacillus pumilus and application thereof | |
| CN103146610A (en) | Plant growth-promoting rhizobacteria and application thereof | |
| CN115011510A (en) | Salt-tolerant growth-promoting stenotrophomonas maltophilia, microbial agent thereof and application thereof | |
| CN108148786B (en) | Bacillus bacteria NJAU-5 capable of effectively promoting crop growth and biological seedling culture substrate developed by same | |
| CN113088471B (en) | Rhizobium prazobium X2 for producing IAA and CMC enzymes and application thereof | |
| CN104593301B (en) | One plant of wall bacillus G1 and its preparation method and application | |
| CN112980748A (en) | Brevibacillus brevis, application and method for producing humic acid by converting lignite | |
| CN116333937B (en) | Plant rhizosphere probiotic strain with greenhouse gas nitrous oxide emission reduction function and application thereof | |
| CN116904361A (en) | Copper bacteria strain with phosphate dissolving capability and application thereof | |
| CN117603832A (en) | Multifunctional nitrogen-fixing growth-promoting bacterium, culture medium, screening and application | |
| CN115477559B (en) | Soil improvement microbial agent and preparation method and application thereof | |
| CN114164140B (en) | Efficient phosphorus-dissolving bacteria MQR6 and fermentation product and application thereof | |
| CN117229983B (en) | Nitrosogenic paraburkholderia PX-418, microbial inoculum and application thereof | |
| CN113817625B (en) | Flavobacterium acidophilus and application thereof in improvement of saline-alkali soil | |
| CN114717125B (en) | Thermophilic bacillus licheniformis AMCC101380 and application thereof in high-temperature composting of tail vegetables | |
| CN112011487B (en) | Aroma-like bacterial strain C40 capable of degrading phenol and having growth promoting function and application thereof | |
| CN110628674B (en) | Bacillus pumilus with functions of improving acid soil and removing potassium and preparation and application of microbial inoculum thereof | |
| CN113980863B (en) | Bacillus siamensis and application thereof | |
| CN114806924B (en) | Achromobacter denitrificans and application thereof | |
| CN118374399A (en) | Planting method for improving rice yield | |
| CN115927053A (en) | Cadmium-resistant fiber microbe Cd-1 and culture method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |