CN117158493B - Pu' er cordyceps white tea and preparation method thereof, and shaking box - Google Patents
Pu' er cordyceps white tea and preparation method thereof, and shaking box Download PDFInfo
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- CN117158493B CN117158493B CN202310841285.0A CN202310841285A CN117158493B CN 117158493 B CN117158493 B CN 117158493B CN 202310841285 A CN202310841285 A CN 202310841285A CN 117158493 B CN117158493 B CN 117158493B
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Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/90—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in food processing or handling, e.g. food conservation
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- Medicines Containing Plant Substances (AREA)
Abstract
The application provides puer cordyceps sinensis white tea, a preparation method thereof and a shaking box, wherein the preparation method comprises the following steps: (1) preparing puer white tea; (2) preparing a cordycepin solution: (3) Spraying the cordycepin solution prepared in the step (2) on the Pu 'er white tea prepared in the step (1) in a mist form, wherein the cordycepin content in the Pu' er white tea is 0.0098% -0.02% by mass. The application adds a proper amount of cordycepin into the Pu ' er white tea, the cordycepin has the functions of improving immunity, regulating blood fat, reducing blood sugar and improving anti-fatigue capability, the theaflavin has the functions of resisting oxidation, preventing and treating cardiovascular diseases, reducing blood fat, resisting cancer, preventing the human body from taking excessive cordycepin by controlling the content of the cordycepin in the Pu ' er tea, preventing the theaflavin in the tea from being damaged by excessive cordycepin, ensuring that the cordycepin and the theaflavin contained in the Pu ' er tea have the functions of mutual assistance and synergy, and retaining the original color, smell and taste of the tea.
Description
Technical Field
The application relates to the technical field of tea preparation, in particular to Pu' er cordyceps sinensis white tea, a preparation method thereof and a shaking box.
Background
The tea leaves used in the tea are generally processed from leaves of tea trees, and the traditional tea leaves are of various types and comprise green tea, black tea, puer tea, scented tea, black tea, white tea and the like. Pu 'er tea is mainly produced in Xishuangbanna, lincang and Pu' er areas of Yunnan province. Pu' er tea is rich in drinking method, and can be drunk either by cleaning or mixing. The puer tea soup is orange and thick, has high and sharp lasting fragrance, unique flavor and mellow taste, is durable and durable, and meanwhile, the puer tea also has the functions of losing weight, reducing blood fat, preventing and treating arteriosclerosis, preventing and treating coronary heart disease, reducing blood pressure, resisting aging, resisting cancer, helping digestion, dispelling alcohol effect and promoting urination, so that the puer tea soup is deeply favored by people, and in order to avoid single tea taste, people generally like to add other traditional Chinese medicine components into puer tea;
cordyceps militaris is also called Cordyceps militaris, cordyceps militaris or Cynomorium songaricum, belonging to the genus Cordyceps of the order Hypocreaceae, ascomycetes, and being medicinal fungus combined by insects and bacteria, and mainly grows in northern area of China. Modern researches have proved that Cordyceps militaris contains cordycepin (3' -deoxyadenosine), cordycepic acid (D-mannitol), adenosine, cordyceps polysaccharide, ergosterol, superoxide dismutase (SOD) and selenium (Se), and has effects of nourishing lung, invigorating kidney, stopping bleeding, resolving phlegm, dilating trachea, tranquilizing mind, resisting various bacteria, and lowering blood pressure.
However, at present, both the Pu' er tea and the cordyceps militaris are directly mixed for drinking, the content of the cordyceps militaris cannot be controlled, the content of the cordyceps militaris is easily high due to mixed drinking, the excessive intake of the cordyceps militaris is easily influenced by human bodies, and the cordyceps militaris and the substances which are extremely easy to damp can not reach ideal preservation conditions and are easy to mildew in the daily preservation process under specific preservation conditions.
Disclosure of Invention
The main purpose of the application is to provide a preparation method of Pu 'er Cordyceps white tea, which can control the cordycepin content in Pu' er tea and avoid poisoning of human body caused by excessive intake of cordycepin.
The application also aims at providing the Pu' er cordyceps sinensis white tea prepared by the preparation method.
The application further aims at providing a shake box applied to the preparation method of the Pu' er cordyceps sinensis white tea.
In order to achieve the above object, the present application provides the following technical solutions:
as a first aspect, the present application relates to a method for preparing puer cordyceps white tea, comprising the steps of:
(1) Preparing puer white tea: selecting fresh leaves of Yunnan big leaves, drying the obtained fresh leaves of Yunnan big leaves in the shade under the environment of no water, light shielding and ventilation, placing the dried leaves in a shaking box for wall breaking treatment, drying in the shade for the second time until the water retention of the tea leaves is lower than 5%, collecting the leaves in a bin, and naturally returning water until the water retention of the tea leaves is 8% -12%;
(2) Preparing a cordycepin solution: freeze-drying and crushing Cordyceps militaris, adding the crushed Cordyceps militaris powder into water according to a preset proportion, stirring and mixing, and stirring for 3-5d to obtain cordycepin solution;
(3) Spraying the cordycepin solution prepared in the step (2) on the Pu ' er white tea prepared in the step (1) in a mist form, uniformly spraying for a plurality of times through turn-over, standing and oxidizing for 24 hours, wherein the water retention capacity of the tea leaves of the obtained Pu ' er Cordyceps white tea is lower than 15%, and the cordycepin content in the Pu ' er Cordyceps white tea is 0.0098% -0.02% by mass.
Further set up: and (3) the treatment time of the tea leaves dried in the shade in the step (1) in the shaking box is 8-10min.
Further set up: the ratio of the cordyceps militaris powder to the water in the step (2) is 0.001-0.005g:100g.
Further set up: the water mixed with Cordyceps militaris powder is mineral water or spring water.
Further set up: before cordycepin is sprayed in the step (3), electrostatic screening treatment is carried out on the Pu 'er white tea, and the treated Pu' er white tea is laid flat and dispersed.
Further set up: the whole preparation process is carried out in a sterile environment.
Further set up: also included is packaging after step (3), including cookie or bulk bag packaging.
As a second aspect, the present application relates to a Pu 'er Cordyceps sinensis white tea prepared by the preparation method of Pu' er Cordyceps sinensis white tea as described above, which is characterized by comprising Pu 'er white tea and cordycepin, wherein the cordycepin accounts for 0.0098% -0.02% of the total mass of Pu' er Cordyceps sinensis white tea.
The application relates to a shake box, which is applied to the preparation method of the Pu' er cordyceps sinensis white tea, and is characterized by comprising a frame, a rotating support and a driving mechanism, wherein the rotating support and the driving mechanism are arranged on the frame, the rotating support is obliquely arranged on the frame, a spiral conveying track is arranged in the rotating support and positioned at the rotating axis of the rotating support, the spiral conveying track can rotate relative to the rotating support, the spiral conveying track is used for conveying tea leaves positioned at the bottom of the rotating support towards the top of the rotating support, and the driving mechanism is respectively connected with the rotating support and the spiral conveying track to respectively drive the rotating support and the spiral conveying track to rotate.
Further set up: the driving mechanism comprises a driving motor and a coaxial linkage piece, the coaxial linkage piece comprises a first rotating shaft and a second rotating shaft, the first rotating shaft is coaxially connected with the rotating support, the second rotating shaft is arranged to be a hollow shaft, the second rotating shaft penetrates through the first rotating shaft to be connected with the spiral conveying track inside the rotating support, the second rotating shaft is connected with an output shaft of the driving motor, a second toothed ring with inner teeth is arranged on the second rotating shaft, a second gear is meshed with the second toothed ring, a first toothed ring with inner teeth is arranged on the first rotating shaft, the first toothed ring is meshed with the first gear, and the first gear is meshed with the second gear.
Compared with the prior art, the scheme of the application has the following advantages:
1. The Pu ' er cordyceps white tea disclosed by the application takes Pu ' er white tea and cordycepin as main components, the total mass of the cordycepin Pu ' er white tea is controlled to be 0.0098% -0.02%, a proper amount of cordycepin has the functions of improving immunity, regulating blood fat, reducing blood sugar and improving fatigue resistance, and theaflavin has the functions of resisting oxidation, preventing cardiovascular diseases, reducing blood fat, resisting cancer, preventing cancer and the like, and the content of cordycepin in Pu ' er tea is controlled to prevent a human body from taking excessive cordycepin, and also prevent theaflavin in the tea from being damaged by excessive cordycepin, so that the cordycepin and the theaflavin contained in Pu ' er tea play roles of mutual assistance and synergistic interaction, and the original color and flavor of the tea are reserved.
2. According to the preparation method of the Pu 'er cordyceps sinensis white tea, the shaking box is adopted to break the wall of the tea in the preparation process of the Pu' er white tea, the cell walls of the tea are broken, precipitation of tea juice in the tea is facilitated, the drying speed of the tea in the shade can be accelerated, the natural cooling time is shortened, and meanwhile, cracks formed by the breaking of the tea in rolling of the shaking box are beneficial to follow-up infiltration of cordycepin solution spray.
3. According to the preparation method of the Pu' er cordyceps white tea, a freeze-drying technology is introduced into a cordycepin solution, the freeze-dried cordyceps militaris is loose and fragile in structure, workers can roll and crush the cordyceps militaris to form powdery particles conveniently, the activity and effective nutritional ingredients of the freeze-dried cordyceps militaris can be fully reserved, and the loss of cordycepin is reduced.
Additional aspects and advantages of the application will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the application.
Drawings
The foregoing and/or additional aspects and advantages of the application will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings, in which:
FIG. 1 is a process flow diagram of a preparation method of Pu' er Cordyceps sinensis white tea of the application;
FIG. 2 is a schematic diagram of an embodiment of a shake flask of the application;
Fig. 3 is a schematic view of a driving mechanism in the shake flask according to the application.
In the figure, 1, a rack; 2. a rotating bracket; 3. a spiral conveying track; 4. a driving mechanism; 41. a driving motor; 42. a coaxial linkage; 421. a first rotation shaft; 422. a second rotation shaft; 423. a first toothed ring; 424. a first gear; 425. a second toothed ring; 426. a second gear; 5. a housing.
Detailed Description
Embodiments of the present application are described in detail below, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to like or similar elements or elements having like or similar functions throughout. The embodiments described below by referring to the drawings are illustrative only and are not to be construed as limiting the application.
The application discloses a preparation method of Pu ' er Cordyceps white tea, which comprises the steps of directly spraying a solution containing cordycepin after wall breaking treatment of Pu ' er white tea, so that the cordycepin contained in Pu ' er tea can be controlled within a reasonable range, the cordycepin and theaflavin contained in Pu ' er tea play roles of mutual assistance and synergy, and the drinking efficacy of Pu ' er tea is improved.
The Pu 'er cordyceps white tea mainly comprises Pu' er white tea and cordycepin, the total mass of the cordycepin Pu 'er white tea is 0.0098% -0.02%, a proper amount of cordycepin has the functions of improving immunity, regulating blood fat, reducing blood sugar and improving fatigue resistance, excessive cordycepin can cause liver injury, excessive cordycepin can influence the content of theaflavin in Pu' er tea, and the theaflavin has the functions of resisting oxidation, preventing and treating cardiovascular diseases, reducing blood fat, resisting cancer, preventing cancer and the like, so that the human body is prevented from taking excessive cordycepin by controlling the content of cordycepin in Pu 'er tea, the theaflavin in the tea is prevented from being damaged by excessive cordycepin, the mutual auxiliary and synergistic effects of the cordycepin and the theaflavin in Pu' er tea are ensured, and the original color and fragrance of the tea are reserved.
Referring to fig. 1, the preparation method of the Pu' er Cordyceps sinensis white tea of the application specifically comprises the following steps:
(1) Pu' er white tea is prepared. The method comprises the steps of taking fresh leaves of Yunnan big-leaf sun-dried green tea as raw materials, firstly drying the obtained fresh leaves of Yunnan big-leaf sun-dried green tea in the environment of no water, avoiding light and ventilation, drying in the shade for 7-8 hours, enabling the surfaces of the fresh leaves to be free of clear water, then placing the dried tea leaves into a shaking box for rolling to break wall, continuing for 7-8 minutes, then carrying out secondary drying in the shade on the tea leaves subjected to the shaking box wall breaking treatment, naturally spreading and cooling until the water content of the tea leaves is not more than 5%, collecting bins after the secondary drying in the shade, and carrying out natural moisture regaining until the water content of the tea leaves reaches 8% -12%, and the natural moisture regaining time is 7-8d, thus obtaining the puer white tea.
The tea leaves which are dried in the shade are put into the shaking box to roll, the tea leaves which roll in the shaking box collide with each other, so that fibers in the tea leaves are damaged in the collision, cell walls are broken, part of tea juice is exuded, moisture contained in the tea leaves is quickly evaporated, and nutritional ingredients in the tea leaves can be reserved to the greatest extent after wall breaking.
Please combine fig. 2 and fig. 3, the shake-box that involves in this step includes frame 1 and locates runing rest 2, actuating mechanism 4 on frame 1, runing rest 2 is set up obliquely on frame 1, actuating mechanism 4 with runing rest 2 is connected, so under actuating mechanism 4's drive, runing rest 2 can rotate relative frame 1, runing rest 2 inside is located its axis of rotation department and is equipped with spiral delivery track 3, actuating mechanism 4 also with spiral delivery track 3 connects, spiral delivery track 3 can relative runing rest 2 rotates, then will be located by the runing rest 2 bottom tealeaves is transported towards runing rest 2's top through the spiral delivery structure for tealeaves can roll many times, greatly increased the collision between the blade, between blade and the runing rest 2 inner wall, improved the broken wall effect of tealeaves.
Specifically, the rotary support 2 adopts the bamboo support, be equipped with the filter screen that sets up along its outline on the rotary support 2 to cover the space on the rotary support 2, avoid big tealeaves to break away from rotary support 2 from the space of rotary support 2, simultaneously, the filter screen again can screen out the piece that tealeaves produced in the collision of rolling. Further, the shake box further comprises a shell 5 covered on the periphery of the rotating support 2, and the shell 5 can receive tea scraps screened out in the rotating process of the rotating support 2 and can also protect the rotating support 2. The shell 5 is provided with an opening and a sealing cover, the sealing cover is hinged on the shell 5 for covering the opening, a worker can add tea leaves into the rotary support 2 or take the tea leaves out of the rotary support from the opening of the shell 5, and the tea leaves can be further processed by screening scraps through the filter screen.
The driving mechanism 4 includes a driving motor 41 and a coaxial linkage 42, and since the rotating bracket 2 and the screw conveyor rail 3 are coaxially disposed, the rotating bracket 2 and the screw conveyor rail 3 are connected to each other by the coaxial linkage 42, and thus the rotating bracket 2 and the screw conveyor rail 3 can be rotated relatively by one set of driving motor 41.
The rotating support 2 in this embodiment and the spiral conveying track 3 rotate in opposite directions, the coaxial linkage 42 includes a first rotating shaft 421 coaxially connected with the rotating support 2 and a second rotating shaft 422 coaxially connected with the spiral conveying track 3, the first rotating shaft 421 is provided as a hollow shaft, the second rotating shaft 422 passes through the first rotating shaft 421 to be connected with the spiral conveying track 3 located inside the rotating support 2, the other end of the second rotating shaft 422 is connected with the output shaft of the driving motor 41, a second toothed ring 425 with internal teeth is provided on the second rotating shaft 422, the second toothed ring 425 is meshed with a second gear 426, a first toothed ring 423 with internal teeth is also provided on the first rotating shaft 421, the first toothed ring 423 is meshed with a first gear 424, and the first gear 424 is meshed with the second gear 426. The driving motor 41 drives the second rotating shaft 422 to rotate, the second toothed ring 425 rotates coaxially with the second rotating shaft 422 and drives the second gear 426 meshed with the second toothed ring 425 to rotate, the rotation direction of the second gear 426 is the same as the rotation direction of the second toothed ring 425, meanwhile, the first gear 424 meshed with the second gear 426 rotates and the rotation direction is opposite to the rotation direction of the second gear 426, the first toothed ring 423 rotates along with the rotation of the first gear 424, the rotation direction of the first toothed ring 423 is consistent with the rotation direction of the first gear 424, the rotation direction of the first toothed ring 423 is opposite to the rotation direction of the second toothed ring 425, namely, the rotation direction of the first rotating shaft 421 and the second rotating shaft 422 is opposite to the rotation direction of the rotating support 2 and the spiral conveying track 3. Moreover, the rotary support 2 is rotating, and the spiral conveying track 3 continuously conveys tea leaves located at the bottom of the rotary support 2 towards the top of the rotary support 2, so that the tea leaves can be continuously rolled, collisions among the tea leaves, between the tea leaves and the inner wall of the rotary support 2 and between the tea leaves and the spiral conveying track 3 are increased, the wall breaking effect of the tea leaves is improved, and the subsequent effect of mixing with cordycepin is ensured.
In addition, the relative rotation speed of the rotary support 2 and the spiral conveying track 3 in the embodiment can be realized by changing the gear ratio of the first gear 424 and the second gear 426, and different rotation speeds can generate different collision effects on tea leaves.
In other embodiments, when the rotation direction of the rotating bracket 2 is the same as that of the spiral conveying track 3, the first gear 424 and the second gear 426 are driven by the synchronous belt, so that the rotation directions of the first gear 424 and the second gear 426 are consistent, the purpose that the rotation direction of the rotating bracket 2 is the same as that of the spiral conveying track 3 can be achieved, and the relative rotation speed of the first gear 424 and the second gear 426 can be adjusted by changing the diameter ratio of the synchronous wheels of the two gears.
Of course, the rotary bracket 2 and the rotary shaft of the screw conveying mechanism of the present application can be driven by two sets of motors, respectively, at a sufficient cost.
The cell walls in the tea leaves treated by the shaking box are damaged, part of tea juice is separated out, the drying speed of the tea leaves in the shade is increased, and favorable conditions are provided for the subsequent full mixing with cordycepin.
(2) Preparing cordycepin. The purchased cordyceps militaris is subjected to freeze-drying and crushing by adopting a freeze-drying technology, and particularly, a cordyceps militaris freeze dryer can be adopted for carrying out freeze-drying treatment on the cordyceps militaris. Cleaning purchased cordyceps militaris, putting the cleaned cordyceps militaris into a cordyceps militaris freeze dryer, and automatically operating the cordyceps militaris freeze dryer according to freeze-drying process technical data such as preset pre-freezing temperature, sublimation drying temperature, vacuum degree, operation time and the like so as to gradually evaporate water in the cordyceps militaris, thereby obtaining the freeze-dried cordyceps militaris. The prefreezing temperature is-40 ℃, the freeze-dried cordyceps militaris structure is loose and fragile, so that workers can conveniently crush and crush the cordyceps militaris to form powdery particles, the activity and effective nutritional ingredients of the freeze-dried cordyceps militaris can be fully reserved, and the loss of cordycepin is reduced. And then mixing and stirring the crushed cordyceps militaris powder with water according to a preset proportion, and stirring for 3-5 days to obtain the cordycepin solution.
The ratio of the cordyceps militaris powder to the water is 0.001-0.005g:100g, wherein the adopted water is mineral water or mountain spring water to prevent the active ingredients in the cordyceps militaris powder from being destroyed, and the mixed solution is continuously stirred to prevent the solution from forming suspension or precipitation, so that the cordyceps militaris powder is fully dissolved in the water, and the cordycepin solution is obtained through 3-5d oxidation reaction.
(3) Spraying the cordycepin solution prepared in the step (2) onto the Pu 'er white tea prepared in the step (1) in a mist form, controlling the spraying dosage and the spraying time to uniformly spray the cordycepin solution on the Pu' er white tea, turning over and uniformly spraying after single-sided spraying is finished, so as to ensure that the surface of the Pu 'er white tea is uniformly and completely adhered to the cordycepin solution, repeating spraying for multiple times, standing and oxidizing for 24 hours, so that the Pu' er white tea thoroughly absorbs cordycepin, and the obtained Pu 'er white tea has a tea water retention less than 15%, and the cordycepin in the Pu' er white is 0.0098% -0.02% in percentage by mass.
After the Pu ' er white tea is subjected to wall breaking treatment of the shaking box, a plurality of tortoise cracks are formed on the blades of the Pu ' er white tea, and a spray mode is adopted to spray the cordycepin solution onto the Pu ' er white tea, so that the cordycepin spray has small particles and can quickly enter the cracks of the Pu ' er white tea to be fully absorbed by the Pu ' er white tea.
In addition, before the cordycepin solution is sprayed, electrostatic screening treatment is carried out on the Pu 'er white tea, and the treated Pu' er white tea is laid flat and dispersed. In the process of processing the puer white tea, because the mutual collision friction between the tea leaves can generate static electricity, the static electricity can enable the tea leaves to carry dust and sundries, therefore, the tea leaves pass through the electrode plate, the charged impurities on the tea leaves can be adsorbed by positive charges on the electrode plate, thereby achieving the classical impurity removal effect on the tea leaves, screening after static electricity treatment, screening out the tea leaves meeting the specification, carrying out the next procedure, and spreading and dispersing the puer white tea to be favorable for evenly spraying the subsequent cordycepin solution onto the puer white tea.
(4) And (5) packaging. The Pu' er Cordyceps white tea is packaged by cake pressing or bulk bag packaging, and is convenient for sale.
The preparation process of the Pu 'er Cordyceps white tea is carried out in a sterile workshop, and bacteria and microorganisms in the air can be killed by using the sterile workshop, so that the sanitary safety and quality of the Pu' er Cordyceps white tea product are ensured, the pollution and errors in the production process are reduced, and the working efficiency is effectively improved.
The Pu 'er cordyceps white tea disclosed by the application takes Pu' er white tea and cordycepin as main components, theanine contained in the Pu 'er white tea can cooperate with caffeine to better promote the release of central brain dopamine, has the effects of promoting refreshment, and also has the effects of sterilizing, strengthening teeth, improving eyesight, protecting eyes, protecting liver and reducing blood lipid and blood pressure, wherein theaflavin is one of main flavonoid substances of the Pu' er tea, has a relatively strong antioxidation effect, and can reduce oxidative damage of human cells. Meanwhile, the added cordycepin has good antibacterial and antiviral effects, can inhibit the growth of pathogenic bacteria such as streptococcus, bacillus melitensis, bacillus anthracis, pig hemorrhagic septicemia, staphylococcus and the like, has the inhibition effect on HIV-I infection of human immunodeficiency virus and the activity of reverse transcriptase of the HIV-I infection, can strongly inhibit lymphocyte and nuclear mononuclear cells, and can prevent the organism from excessive immunopathogenic injury, so that the cordycepin can carry out immunity regulation on the organism. In addition, cordycepin can inhibit protein kinase activity and resist cleavage of glycosyl by nucleoside phosphorylase. Has effects in regulating humoral immunity, increasing spleen weight, and accelerating liver nucleic acid and protein update; has effects in resisting anoxia, increasing nutrient and blood flow of heart, reducing serum cholesterol, increasing coronary blood flow, reducing coronary, brain and peripheral vascular resistance, inhibiting nucleic acid methylation, and preventing cerebral thrombosis and cardiovascular diseases. The free radicals can make people more easily age, and the cordycepin has stronger scavenging effect on the free radicals. SOD component contained in Cordyceps militaris is typical antioxidant, and has antioxidant effect by scavenging free radicals in vivo and inhibiting lipid peroxidation.
However, when the cordycepin content is too high, cytotoxicity can be caused in fungi, and excessive eating can cause dizziness, chest distress, dry mouth and tongue and other discomforts, so the application combines the following experimental groups to illustrate that the cordycepin content is used for the Pu' er cordyceps white tea.
Experiment group one
A Pu ' er Cordyceps white tea comprises Pu ' er white tea and cordycepin, wherein the cordycepin accounts for 0.0098% of the total mass of Pu ' er Cordyceps high white tea.
The preparation method of the Pu' er cordyceps white tea comprises the following steps:
(1) Preparing puer white tea, selecting fresh leaves of Yunnan big leaves, drying the obtained fresh leaves of Yunnan big leaves in the shade for 8 hours in an anhydrous, light-proof and ventilated environment, placing the dried fresh leaves in a shaking box, rolling for 8 minutes to perform wall breaking treatment, drying in the shade for the second time until the water retention capacity of tea leaves is lower than 5%, collecting a bin, and naturally returning water until the water retention capacity of the tea leaves is 8% -12%, wherein the natural water return time is 8d.
(2) Preparing a cordycepin solution: freeze-drying and crushing cordyceps militaris, and mixing 0.001g of crushed cordyceps militaris powder: 100g of the cordycepin is added into mineral water and stirred for 3 days to obtain cordycepin solution.
(3) Spraying the cordycepin solution prepared in the step (2) on the Pu ' er white tea prepared in the step (1) in a mist form, circularly turning over and uniformly spraying for three times, standing and oxidizing for 24 hours, wherein the water retention of the tea leaves of the obtained Pu ' er Cordyceps white tea is 15%, and the cordycepin content in the Pu ' er Cordyceps white tea is 0.0098% in percentage by mass.
Experiment group II
A Pu ' er Cordyceps white tea comprises Pu ' er white tea and cordycepin, wherein the cordycepin accounts for 0.02% of the total mass of Pu ' er Cordyceps high white tea.
The preparation method of the Pu' er cordyceps white tea is different from the experiment group I in that the cordyceps militaris powder obtained by crushing in the step (2) is prepared according to 0.005g:100g of the cordycepin is added into mineral water and stirred and mixed for 5 days to obtain cordycepin solution, and the content of cordycepin in the finally obtained Pu' er cordyceps white tea is 0.02% in percentage by mass.
Experiment group III
A Pu ' er Cordyceps white tea comprises Pu ' er white tea and cordycepin, wherein the cordycepin accounts for 0.015% of the total mass of Pu ' er Cordyceps high white tea.
The preparation method of the Pu' er cordyceps white tea is different from the experiment group I in that the cordyceps militaris powder obtained by crushing in the step (2) is prepared according to 0.003g:100g of the cordycepin is added into mineral water and stirred and mixed for 5 days to obtain cordycepin solution, and the content of cordycepin in the finally obtained Pu' er cordyceps white tea is 0.015% in percentage by mass.
Control group one
The Pu' er white tea directly obtained in the step (1) does not contain cordycepin.
The preparation method of the puer white tea comprises the following steps: selecting fresh leaves of Yunnan big leaves, drying the obtained fresh leaves of Yunnan big leaves in the shade for 8 hours in an environment without water, avoiding light and ventilating, placing the dried leaves in a shaking box, turning over and rolling for 8 minutes to perform wall breaking treatment, drying in the shade for the second time until the water retention of tea leaves is lower than 5%, collecting bins, and naturally returning water until the water retention of the tea leaves is 8% -12%, wherein the natural water return time is 8d.
Control group two
A Pu ' er Cordyceps white tea comprises Pu ' er white tea and cordycepin, wherein the cordycepin accounts for 0.04% of the total mass of Pu ' er Cordyceps high white tea.
The preparation method of the Pu' er cordyceps white tea is different from the experiment group I in that the cordyceps militaris powder obtained by crushing in the step (2) is prepared according to 0.01g:100g of the cordycepin is added into mineral water and stirred and mixed for 5 days to obtain cordycepin solution, and the content of cordycepin in the finally obtained Pu' er cordyceps white tea is 0.04% in percentage by mass.
Example 1
The five groups of tea leaves were subjected to quality sensory evaluation, and the puer tea prepared in the experimental groups 1 to 3 and the comparative examples 1 to 2 were subjected to quality sensory evaluation by 20 trained sensory evaluators; the evaluation results are shown in Table 1. In the quality sensory evaluation process, a seven-degree method in fuzzy mathematics is adopted to analyze the scoring value, and the comment domain is as follows: 1. the most unacceptable, 2, more unacceptable, 3, slightly unacceptable, 4, barely acceptable, 5, slightly acceptable, 6, easily acceptable, 7, most acceptable; the evaluation field comprises: taste, smell and posture. Wherein, the taste is smooth in mouth and the tea taste is fragrant and most acceptable; the smell is most acceptable with no obvious special pungent smell; the posture was most acceptable with no significant change in color upon standing for 6 months.
And after the sensory evaluator assessed one product, rinse with clear water at intervals of 10min before assessing the next product.
Table 1 quality sensory evaluation table
| Taste of the tea | Smell of | Posture of body | |
| Experiment group one | 6.6 | 6.5 | 6.7 |
| Experiment group II | 6.2 | 6.0 | 6.1 |
| Experiment group III | 6.0 | 5.9 | 5.9 |
| Control group one | 6.9 | 6.9 | 6.9 |
| Control group two | 4.8 | 5.3 | 5.0 |
As can be seen from the data in table 1, the Pu 'er Cordyceps sinensis high-whiteness tea prepared in the experimental groups one to three has good taste, smell and body state of Pu' er white tea in the comparative example one without cordycepin is better than that of Pu 'er Cordyceps sinensis white tea with cordycepin, the cordycepin in the Pu' er Cordyceps white tea in the comparative example two is far more than the cordycepin content range in the application, the taste and smell are greatly reduced, the body state is not as good as that in other experimental groups, and the taste, smell and body state of tea soup are usually dependent on theaflavins contained in tea, namely, the addition of excessive cordycepin proves that the formation of theaflavins is caused, and the taste and smell of cordyceps militaris are more stimulated, and the addition of excessive cordycepin also affects the taste of tea, so that drinkers can feel worse.
Example two
The five groups of tea leaves are used for detecting the anti-fatigue function, mice are fed in an adaptive mode through a mouse animal experiment, the mice are grouped randomly, and sterile water and sterile feed are used for feeding during the experiment. Mice were randomly divided into 6 groups, namely, a blank control group (gastric normal saline, continuous gastric lavage for 30 days), a first experimental group administration group, a second experimental group administration group, a third experimental group administration group, a first control group administration, and a second control group administration, and were continuously gastric lavaged for 30 days, and the mice were free to ingest and drink water during the experiment. The experiments of weight-bearing swimming, serum urea, liver glycogen and blood lactic acid content measurement are respectively carried out according to the technical specifications of health food inspection and evaluation and are shown in table 2. The weight bearing swimming experiment of the application mainly uses lead wires to bind the tail parts of the mice to bear 5% of weight, and the lead wires are placed in a swimming box to record the time from the beginning of the swimming of the mice to death. A circular swimming box is adopted, the caliber is 40cm, the water temperature is 25 ℃, and the water depth is more than 30cm.
Table 2 anti-fatigue test data
As can be seen from table 2 above, the mice of the control group two were in a toxic state due to intake of excessive cordycepin, while the swimming time of the experimental group one to three and the control group was significantly prolonged compared with that of the blank control group, and prolonged with increase of the intake of the effective components, indicating increase of the effective content, and improvement of the anti-fatigue effect. And urea in blood has a certain clearing effect after the mice in the experimental group one to three pairs move; liver glycogen is taken as an important energy source, and the contents of the liver glycogen can be improved in one to three experimental groups, so that the anti-fatigue effect is achieved; blood lactic acid is used as a main metabolite of muscle exercise, the more the lactic acid is accumulated, the more the fatigue degree is, the lactic acid generated by muscle in the exercise process permeates into blood, the accumulation of lactic acid in blood can be obviously reduced from one experimental group to three experimental groups relative to a blank control group, the blood lactic acid content of mice in each group is not greatly different before swimming, the average lactic acid water after swimming is obviously improved, the blood lactic acid of each group of mice is recovered to different degrees after 20 minutes of rest, the metabolism of lactic acid can be accelerated, and the anti-fatigue effect is achieved. The control group 1 also had a certain anti-fatigue effect, but the effect was significantly inferior to that of the experimental groups one to three.
Example III
The five groups of tea leaves are used for carrying out immunity improvement experiments, specifically, mice are fed in an adaptive mode through a mouse animal experiment, the mice are grouped randomly, and sterile water and sterile feed are used for feeding during the experiment. Mice were randomly divided into 6 groups, namely, a blank control group (gastric normal saline, continuous gastric lavage for 30 days), a first experimental group administration group, a second experimental group administration group, a third experimental group administration group, a first control group administration, and a second control group administration, and were continuously gastric lavaged for 30 days, and the mice were free to ingest and drink water during the experiment. According to the technical specifications of health food inspection and evaluation, experiments of the mouse abdominal cavity macrophage phagocytosis of chicken red blood cells, NK cell activity determination and lymphocyte transformation are respectively carried out.
TABLE 3 Table 3
It is obvious from the combination of Table 3 that after the mice drink the tea of the application, the lymphocyte proliferation capacity, phagocytic cell capacity and NK cell activity are obviously improved, the NK cell activity detection result is negative, the lymphocyte proliferation capacity can be used for evaluating the reactivity and immune function of immune cells to antigens, the phagocytic index can reflect the phagocytic capacity of phagocytes, and then the immune capacity of organisms is reflected to a certain extent, the NK cell activity refers to the activity of natural killer cells, and the higher the activity is, the stronger the individual resistance is. Therefore, with the increase of the cordycepin content in the experimental groups one to three, the immunity is also of interest to be gradually enhanced, but from the control group two, the excessive intake of cordycepin can cause cytotoxicity, and the aim of improving the immunity of the organism can not be achieved.
The foregoing is only a partial embodiment of the present application, and it should be noted that it will be apparent to those skilled in the art that modifications and adaptations can be made without departing from the principles of the present application, and such modifications and adaptations are intended to be comprehended within the scope of the present application.
Claims (5)
1. The preparation method of the Pu' er cordyceps white tea is characterized by comprising the following steps of:
(1) Preparing puer white tea: selecting fresh leaves of Yunnan big leaves, drying the obtained fresh leaves of Yunnan big leaves in the shade under the environment of no water, light shading and ventilation, placing the fresh leaves in a shaking box for wall breaking treatment for 8-10min, wherein the shaking box comprises a frame (1) and a rotary support (2) arranged on the frame (1), a driving mechanism (4), the rotary support (2) is obliquely arranged on the frame (1), the rotary support (2) adopts a bamboo support, a filter screen arranged along the outer contour of the rotary support (2) is arranged on the rotary support (2) for covering gaps on the rotary support (2), a spiral conveying track (3) is arranged in the rotary support (2) and positioned at the rotary axis of the rotary support (2), the spiral conveying track (3) can rotate relative to the rotary support (2), the spiral conveying track (3) is used for conveying tea leaves positioned at the bottom of the rotary support (2) towards the top of the rotary support (2), the driving mechanism (4) is respectively connected with the rotary support (2) and the spiral conveying track (3) so as to be coaxial with a first rotary support (42) and a second rotary support (42) which is coaxially connected with a first rotary support (41) and a second rotary support (2), the driving mechanism (421) comprises a coaxial linkage (42), the first rotating shaft (421) is arranged as a hollow shaft, the second rotating shaft (422) passes through the first rotating shaft (421) to be connected with a spiral conveying track (3) positioned in the rotating support (2), the second rotating shaft (422) is connected with an output shaft of the driving motor (41), a second toothed ring (425) with inner teeth is arranged on the second rotating shaft (422), the second toothed ring (425) is meshed with a second gear (426), the first rotating shaft (421) is provided with a first toothed ring (423) with inner teeth, the first toothed ring (423) is meshed with a first gear (424), the first gear (424) is meshed with the second gear (426), the relative rotation speed of the rotating support (2) and the spiral conveying track (3) is adjusted by changing the tooth ratio of the first gear (424) and the second gear (426), after the wall breaking treatment of a shaking box is finished, the tea leaves are dried for the second time until the water retention is lower than 5%, and then the tea leaves are collected to be naturally 8% -12% -water retention;
(2) Preparing a cordycepin solution: freeze-drying and crushing cordyceps militaris, and mixing the crushed cordyceps militaris powder with cordyceps militaris powder: water = 0.001-0.005g: adding 100g of the cordycepin into water, stirring and mixing, and stirring for 3-5d to obtain cordycepin solution;
(3) Carrying out electrostatic screening treatment on the Pu ' er white tea obtained in the step (1), spreading and dispersing the treated Pu ' er white tea, spraying the cordycepin solution prepared in the step (2) on the spread Pu ' er white tea in a mist manner, uniformly spraying for a plurality of times through turn-over, standing and oxidizing for 24 hours, wherein the water retention of the tea leaves of the Pu ' er Cordyceps white tea is lower than 15%, and the cordycepin content in the Pu ' er Cordyceps white tea is 0.0098% -0.02% in percentage by mass.
2. The method for preparing Pu' er Cordyceps white tea according to claim 1, wherein the water mixed with Cordyceps militaris powder is mineral water or mineral water.
3. The method for preparing Pu' er Cordyceps white tea according to claim 1, wherein the whole preparation process is performed under aseptic environment.
4. The method for preparing Pu' er Cordyceps white tea according to claim 1, further comprising packaging after step (3), wherein the packaging comprises cake or bulk packaging.
5. A Pu' er Cordyceps sinensis white tea as claimed in any one of claims 1 to 4.
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