CN117147546A - Low-value leukocyte specimen concentration smear inspection method - Google Patents
Low-value leukocyte specimen concentration smear inspection method Download PDFInfo
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- CN117147546A CN117147546A CN202311109876.5A CN202311109876A CN117147546A CN 117147546 A CN117147546 A CN 117147546A CN 202311109876 A CN202311109876 A CN 202311109876A CN 117147546 A CN117147546 A CN 117147546A
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- smear
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- 210000000265 leukocyte Anatomy 0.000 title claims abstract description 40
- 238000000034 method Methods 0.000 title claims abstract description 24
- 238000007689 inspection Methods 0.000 title description 4
- 210000004369 blood Anatomy 0.000 claims abstract description 29
- 239000008280 blood Substances 0.000 claims abstract description 29
- 238000010186 staining Methods 0.000 claims abstract description 10
- 210000003743 erythrocyte Anatomy 0.000 claims abstract description 5
- 102000001554 Hemoglobins Human genes 0.000 claims abstract description 4
- 108010054147 Hemoglobins Proteins 0.000 claims abstract description 4
- 238000005119 centrifugation Methods 0.000 claims description 9
- 238000011120 smear test Methods 0.000 claims description 6
- 210000004027 cell Anatomy 0.000 abstract description 14
- 238000003745 diagnosis Methods 0.000 description 4
- 238000009534 blood test Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 208000014951 hematologic disease Diseases 0.000 description 2
- 238000000386 microscopy Methods 0.000 description 2
- 238000003908 quality control method Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000010876 biochemical test Methods 0.000 description 1
- 238000004159 blood analysis Methods 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 238000004820 blood count Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000013399 early diagnosis Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 210000002977 intracellular fluid Anatomy 0.000 description 1
- 238000011005 laboratory method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000013517 stratification Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
- G01N2021/0112—Apparatus in one mechanical, optical or electronic block
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The application discloses a method for checking a concentrated smear of a low-value leukocyte specimen, which comprises the following steps: s1: centrifuging the blood sample to separate a plasma layer, a platelet layer, a leukocyte layer, a reduced hemoglobin layer, and an oxygenated red blood cell layer; s2: accurately sucking the plasma layer and the platelet layer; s3: precisely placing a leukocyte layer on an optically standardized slide and pushing the slide; s4: the white blood cells on the slide were precisely stained according to international staining standards. By the concentrated blood smear examination method, the number of visible white blood cells in each oil scope view is obviously increased, thereby greatly improving the examination efficiency and accuracy of the low-value white blood sample. Improved cell distribution and morphology: after concentrated blood smear examination, the concentration of white blood cells is obviously increased, so that the cell distribution is more uniform and the cell morphology is more typical. This helps the inspector to identify the cells more accurately, reducing the risk of misdiagnosis.
Description
Technical Field
The application relates to the technical field of blood analysis, in particular to a method for checking a concentrated smear of a low-value leukocyte specimen.
Background
In current clinical routine blood tests, blood smear tests are one of the most common test techniques, and traditional blood smear tests are a common clinical laboratory method for assessing the condition of blood cells of a patient.
By searching, chinese patent application CN1605869B discloses a blood test method, which proposes a blood test technique for performing biochemical tests by taking out intracellular fluid components in erythrocytes.
However, in blood smear examination, when the white blood cell count in blood is lower than 2.0X10 9 at/L, the blood smear has few leukocytes, and for low-value leukocyte blood samples, multiple fields of view are often required to be examined to obtain sufficient information, which results in time and effort consuming examination and challenges for diagnostic accuracy.
In addition, due to the sparse distribution of leukocytes in the field of view, and irregularities in cell morphology, the diagnostician typically needs to invest more time and effort to accurately identify and count leukocytes, which can extend patient latency and increase the risk of false diagnosis;
in addition, because the low-value leukocyte blood sample has few cells, the cells are easy to push to the edge of the blood smear when pushing the smear, so that the cell morphology is atypical, and the problems of increased artificial recognition difficulty, misdiagnosis and the like are urgent to be solved.
Disclosure of Invention
The application aims to solve the defects in the prior art, and provides a low-value leukocyte specimen concentration smear inspection method.
In order to achieve the above purpose, the present application adopts the following technical scheme:
a method for concentrated smear examination of a low value leukocyte specimen, comprising the steps of:
s1: centrifuging the blood sample to separate a plasma layer, a platelet layer, a leukocyte layer, a reduced hemoglobin layer, and an oxygenated red blood cell layer;
s2: accurately sucking the plasma layer and the platelet layer;
s3: precisely placing a leukocyte layer on an optically standardized slide and pushing the slide;
s4: the white blood cells on the slide were precisely stained according to international staining standards.
Further, in step 3 and step 4, the pushing and staining steps are in compliance with international blood smear examination criteria.
Further, in step S1, the centrifugation speed is 2000.+ -. 10 rpm and the centrifugation time is 10.+ -. 1 min.
Further, in step S4, white blood cells on the slide are subjected to high-magnification microscopy after staining, with a microscope magnification of 1000x or more.
Compared with the prior art, the application has the beneficial effects that:
increase the efficiency and accuracy of leukocyte detection: by the concentrated blood smear examination method, the number of visible white blood cells in each oil scope view is obviously increased, thereby greatly improving the examination efficiency and accuracy of the low-value white blood sample. This means that the doctor can detect abnormal cells more rapidly, contributing to early diagnosis of blood diseases.
Improved cell distribution and morphology: after concentrated blood smear examination, the concentration of white blood cells is obviously increased, so that the cell distribution is more uniform and the cell morphology is more typical. This helps the inspector to identify the cells more accurately, reducing the risk of misdiagnosis.
Standardization and quality control: the use of international staining standards, ISO9001:2015 quality standards, and CE certified centrifuges, etc. requirements, ensures standardization and quality control of the process. This helps maintain consistency and repeatability of the inspection.
The time and labor are wasted: concentrated blood smear tests significantly reduce the test time and effort on low value leukocyte blood specimens as compared to traditional blood smear tests. This reduces the workload of the medical laboratory.
Solves the problems of time and labor waste in blood smear examination of low-value leucocyte blood specimens, and is helpful for improving disease diagnosis and patient care.
Drawings
The accompanying drawings are included to provide a further understanding of the application and are incorporated in and constitute a part of this specification, illustrate the application and together with the embodiments of the application, serve to explain the application.
FIG. 1 is a schematic diagram of blood stratification after centrifugation in an embodiment of the present application;
FIG. 2 is a schematic diagram of the application in comparison to a conventional blood smear test.
Detailed Description
The following description of the embodiments of the present application will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present application, but not all embodiments.
Referring to fig. 1, the method for concentrated smear examination of low value leukocyte specimens comprises the steps of:
s1: centrifuging the blood sample to separate a plasma layer, a platelet layer, a leukocyte layer, a reduced hemoglobin layer, and an oxygenated red blood cell layer;
s2: accurately sucking the plasma layer and the platelet layer;
s3: precisely placing a leukocyte layer on an optically standardized slide and pushing the slide;
s4: the white blood cells on the slide were precisely stained according to international staining standards.
In this embodiment, the method can obtain high quality white blood cell images in blood samples by precisely controlling the centrifugal speed and time and using international staining standards, which is helpful for rapid and accurate diagnosis of various blood diseases.
In a specific embodiment of the application, in step 3 and step 4, the pushing and staining steps are in compliance with international blood smear examination criteria; wherein, the slide glass is compounded with ISO9001:2015 quality standard.
It should be noted that the method is ensured to be consistent with the traditional international blood smear examination standard, so that the medical professional can easily adopt the method without additional training. This is beneficial in facilitating the widespread adoption and standardization of the process.
In a specific embodiment of the present application, in step S1, the centrifugation speed is 2000.+ -.10 rpm and the centrifugation time is 10.+ -.1 min.
It should be noted that by means of an exact centrifugation speed and time frame, the method can be implemented on different centrifugation devices without affecting the consistency of the results. This helps to ensure the reproducibility and reliability of the method.
In a specific embodiment of the present application, in step S4, white blood cells on a slide are subjected to high-magnification microscopy at a microscope magnification of 1000x or more.
It should be noted that, the high-magnification microscopic examination can provide more detailed leukocyte structure information, which is helpful for doctors to evaluate the cell states in blood smears more accurately, thereby improving the diagnosis accuracy of diseases.
As a preferred practice of the application, following the above procedure, the leukocyte layer is concentrated so that 3 to 5 leukocytes are visible in each standardized oily scope field.
For a better understanding of the technical solution of the present application, the following description is further given in connection with the implementation.
The implementation of the scheme and the adoption of the existing smear checking means carry out blood smear checking on the same low-value leukocyte blood specimen, wherein: the result of the oil microscopic examination is shown in figure 2.
The foregoing is only a preferred embodiment of the present application, but the scope of the present application is not limited thereto, and any person skilled in the art, who is within the scope of the present application, should make equivalent substitutions or modifications according to the technical scheme of the present application and the inventive concept thereof, and should be covered by the scope of the present application.
Claims (4)
1. A method for concentrated smear examination of a low value leukocyte specimen, comprising the steps of:
s1: centrifuging the blood sample to separate a plasma layer, a platelet layer, a leukocyte layer, a reduced hemoglobin layer, and an oxygenated red blood cell layer;
s2: accurately sucking the plasma layer and the platelet layer;
s3: precisely placing a leukocyte layer on an optically standardized slide and pushing the slide;
s4: the white blood cells on the slide were precisely stained according to international staining standards.
2. The method according to claim 1, wherein in step 3 and step 4, the pushing and staining steps are in compliance with the international blood smear test standard.
3. The method according to claim 1 or 2, wherein in step S1, the centrifugation speed is 2000±10 rpm and the centrifugation time is 10±1 min.
4. The method according to claim 1, wherein in step S4, the white blood cells on the slide are subjected to high-magnification microscopic examination at a microscopic magnification of 1000x or more.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311109876.5A CN117147546A (en) | 2023-08-30 | 2023-08-30 | Low-value leukocyte specimen concentration smear inspection method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311109876.5A CN117147546A (en) | 2023-08-30 | 2023-08-30 | Low-value leukocyte specimen concentration smear inspection method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117147546A true CN117147546A (en) | 2023-12-01 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311109876.5A Pending CN117147546A (en) | 2023-08-30 | 2023-08-30 | Low-value leukocyte specimen concentration smear inspection method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117147546A (en) |
-
2023
- 2023-08-30 CN CN202311109876.5A patent/CN117147546A/en active Pending
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