CN117123289A - Reagent management system - Google Patents

Abstract

The invention relates to the technical field of medicine, in particular to a reagent management system, wherein a first sealing plate is arranged in a bottle body, and a reagent standby module, a reagent storage module, a pipetting module and a reagent using mechanism are arranged, wherein the reagent standby module is used for temporarily storing a currently required reagent; the reagent storage module is communicated with the reagent standby module, the reagent storage module is used for storing at least one reagent, the storage space of the reagent storage module is in a low-temperature environment, the pipetting module is used for transferring the currently required reagent stored in the reagent storage module to the reagent standby module, and the pipetting module is also used for transferring the currently required reagent temporarily stored in the reagent standby module to the reagent using mechanism for the reagent using mechanism to use, so that the reagent management system has independent temperature control, cleaning and pipetting functions when in use and can realize repeated use.

Description

Reagent management system

Technical field

The invention relates to the field of medical technology, and in particular to a reagent management system.

Background technique

The existing reagent management system can solve the problem of selecting and fluidizing a variety of reagents, but the reagents need to be stored in a specified temperature range (such as the storage temperature of conventional biochemical reaction reagents is 4-8°C). At the same time, after the reagents are sent to the flow cell, It needs to be replaced or cleaned, so the reagent management system needs to be independently designed for temperature control, cleaning and pipetting, and cannot be reused.

Contents of the invention

The main purpose of the present invention is to provide a reagent management system, aiming to solve the technical problems existing in the reagent management system in related technologies that require independent temperature control, cleaning and pipetting designs, and cannot be reused.

In order to achieve the above objects, in the first aspect, the present invention proposes a reagent management system, including:

Reagent standby module, the reagent standby module is used to temporarily store the currently required reagents; wherein, the storage space of the reagent storage module is in a normal temperature environment;

Reagent storage module, the reagent storage module is connected with the reagent standby module, the reagent storage module is used to store at least one reagent, wherein the temperature in the storage space of the reagent storage module is A, A≤8 ℃;

A pipetting module, the pipetting module is used to transfer the currently required reagents stored in the reagent storage module to the reagent standby module; and,

Reagent using mechanism, the pipetting module is also used to transfer the currently required reagents temporarily stored in the reagent standby module to the reagent using mechanism for use by the reagent using mechanism.

Optionally, a reagent standby area for storing the currently required reagents is formed in the reagent standby module, and the reagent standby area is set at normal temperature.

Optionally, a temperature adjustment member is provided on the outer periphery of the reagent storage module, and the temperature adjustment member is used to maintain the temperature of the reagent storage module at A, A≤8°C.

Optionally, a sealed space is formed in the reagent waiting area, and the currently required reagents are temporarily stored in the sealed space through a reagent kit.

Optionally, the reagent pipeline system further includes a degassing module, which is used to degas the space in the reagent box.

Optionally, the reagent management system further includes a cleaning module, which is used to clean the reagent storage module.

Optionally, the cleaning module is provided in the reagent storage module.

Optionally, the reagent standby module, the pipetting module, the reagent using mechanism, the degassing module, the cleaning module and the reagent storage module are arranged in a pry.

Optionally, the pipetting module includes:

horizontal pieces;

a vertical member movably mounted on the horizontal member in a horizontal direction; and,

The pipetting part is movably installed on the vertical part along the vertical direction.

Optionally, the reagent standby module and the pipetting module are connected through a connecting pipeline, and a valve is provided on the connecting pipeline.

The present invention has the following intentional effects:

The present invention sets up a reagent standby module, a reagent storage module, a pipetting module and a reagent using mechanism. The reagent standby module is used to temporarily store the currently required reagents; wherein, the storage space of the reagent storage module is in a normal temperature environment, and the reagent storage module Connected to the reagent standby module, the reagent storage module is used to store at least one reagent, wherein the storage space of the reagent storage module is a low-temperature environment, and the pipetting module is used to transfer the currently required reagents stored in the reagent storage module to The reagent standby module and the pipetting module are also used to transfer the currently required reagents temporarily stored in the reagent standby module to the reagent using mechanism for use by the reagent using mechanism, so that the reagent management system of the present invention can be equipped with Independent temperature control, cleaning and pipetting functions enable repeated use.

Description of the drawings

In order to explain the embodiments of the present invention or the technical solutions in the prior art more clearly, the drawings needed to be used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings in the following description are only These are some embodiments of the present invention. For those of ordinary skill in the art, other drawings can be obtained based on the structures shown in these drawings without exerting creative efforts.

Figure 1 is a schematic structural diagram of a reagent management system according to an example of the present invention.

The realization of the purpose, functional features and advantages of the present invention will be further described with reference to the embodiments and the accompanying drawings.

Detailed ways

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative efforts fall within the scope of protection of the present invention.

It should be noted that all directional indications (such as up, down, left, right, front, back...) in the embodiment of the present invention are only used to explain the relationship between various mechanisms in a specific posture (as shown in the accompanying drawings). Relative positional relationship, movement conditions, etc., if the specific posture changes, the directional indication will also change accordingly.

In the present invention, unless otherwise clearly stated and limited, the terms "connection", "fixing", etc. should be understood in a broad sense. For example, "fixing" can be a fixed connection, a detachable connection, or an integral body; It can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium; it can be an internal connection between two elements or an interactive relationship between two elements, unless otherwise clearly limited. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

In addition, if there are descriptions involving “first”, “second”, etc. in the embodiments of the present invention, the descriptions of “first”, “second”, etc. are only for descriptive purposes and shall not be understood as indications or implications. Its relative importance or implicit indication of the number of technical features indicated. Therefore, features defined as "first" and "second" may explicitly or implicitly include at least one of these features. In addition, the meaning of "and/or" appearing in the entire text includes three parallel solutions. Taking "A and/or B" as an example, it includes solution A, or solution B, or a solution that satisfies both A and B at the same time. In addition, the technical solutions in various embodiments can be combined with each other, but it must be based on the realization by those of ordinary skill in the art. When the combination of technical solutions is contradictory or cannot be realized, it should be considered that such a combination of technical solutions does not exist. , nor within the protection scope required by the present invention.

The inventive concept of the present invention will be further elaborated below in conjunction with some specific embodiments.

The invention proposes a reagent management system.

As shown in Figure 1, an embodiment of the reagent management system of the present invention is proposed.

In this embodiment, please refer to Figure 1. This type of reagent management system includes a reagent standby module, a reagent storage module, a pipetting module and a reagent using mechanism. The reagent standby module is used to temporarily store the currently required reagents; wherein, the reagent The storage space of the storage module is in a normal temperature environment. The reagent storage module is connected to the reagent standby module. The reagent storage module is used to store at least one reagent. The temperature in the storage space of the reagent storage module is A, and A≤8°C. The pipetting module is used to transfer the currently required reagents stored in the reagent storage module to the reagent standby module. The pipetting module is also used to transfer the currently required reagents temporarily stored in the reagent standby module to the reagent using mechanism. For use by reagent user institutions.

In this embodiment, by setting up a reagent standby module, a reagent storage module, a pipetting module and a reagent using mechanism, the reagent standby module is used to temporarily store the currently required reagents; wherein, the storage space of the reagent storage module is in a normal temperature environment. , the reagent storage module is connected with the reagent standby module, the reagent storage module is used to store at least one reagent, wherein the storage space of the reagent storage module is a low-temperature environment, and the pipetting module is used to transfer all the current data stored in the reagent storage module. The required reagents are transferred to the reagent standby module, and the pipetting module is also used to transfer the currently required reagents temporarily stored in the reagent standby module to the reagent using mechanism for use by the reagent using mechanism, so that the present invention can be used when using The reagent management system has independent temperature control, cleaning and pipetting functions and can be reused.

In some specific embodiments, a reagent standby area for storing currently required reagents is formed in the reagent standby module, and the reagent standby area is set at normal temperature. The reagent storage module is provided with a temperature adjustment piece on its outer periphery, and the temperature adjustment piece is used to maintain the temperature of the reagent storage module at A, A≤8°C. A sealed space is formed in the reagent waiting area, and the currently required reagents are temporarily stored in the sealed space through the reagent kit. The reagent pipeline system also includes a degassing module, which is used to degas the space in the reagent box. The reagent management system also includes a cleaning module, which is used to clean the cleaning reagent storage module. The cleaning module is arranged in the reagent storage module. The reagent standby module, pipetting module, reagent using mechanism, degassing module, cleaning module and reagent storage module are arranged in a pry. The pipetting module includes a horizontal part, a vertical part and a pipetting part. The vertical part is movably installed on the horizontal part along the horizontal direction, and the pipetting part is movably installed on the vertical part along the vertical direction. The reagent standby module and the pipetting module are connected through a connecting pipeline, and a valve is provided on the connecting pipeline.

Of course, in some specific embodiments, the reagent management system of the present invention can be implemented with reference to the following examples: Specifically, the reagent management system includes a reagent standby module: a reagent standby area, which is used to temporarily store the currently required reagents. No separate low-temperature conditions and cleaning structures are required, making pipetting and maintenance easier;

Reagent storage module: Reagent storage area, which can store large-capacity reagents. It is relatively independent. When reagents need to be used, it is connected to the reagent substitution area. Pipetting or cleaning can be performed before pipetting to meet the reagent consumption in the waiting area; when it is not needed, When reagents are used, it is used as an independent reagent storage area, separated and stored separately to facilitate long-term storage and avoid cross-contamination;

Pipetting module: It can be integrated into the reagent storage area module, or it can be relatively independent. It operates when it is necessary to pipette reagents from the storage area to the waiting area, connects the storage area and the waiting area, and completes the pipetting;

Automatic cleaning: It can be integrated in the reagent storage area module, or it can be relatively independent. When the reagent standby area needs to be cleaned, the inner wall of the reagent box in the reagent standby area will be cleaned;

Degassing treatment: It can be integrated into the reagent storage area module, or it can be relatively independent. When the reagent standby area needs to be degassed, the inner wall of the kit in the reagent standby area can be degassed;

Working principle and method: The reagent storage module refrigerated stores large-capacity reagents, and the pipetting module takes the reagents from the storage module and moves them to the reagent standby area. The pipetting capacity is determined by the control system, such as the amount of reaction reagent A in one use trip. 200ml; the pipetting method can be a combination of horizontal and vertical, or a turntable movement method;

The degassing module is used to optionally perform degassing when moving reagents from the storage area to the waiting area to achieve the purpose of degassing;

The cleaning module is used to clean the inner wall of the reagent standby area. For example, the bottom of the reagent tube in the standby area is designed as a connectable component. The cleaning reagent is connected to the waste pump and pumped into the reagent tube in the reagent standby area to perform cleaning, such as cleaning the reagent. Pump liquid from the bottom of the reagent tube upward for flushing and soaking. After cleaning, pump the liquid from the bottom and discharge it into the waste liquid bucket to complete the cleaning operation;

Chemical reaction process: synthesis of a strand: the first nucleotide and the first polymerase perform a polymerization reaction to extend the first sequencing primer, allowing the first nucleotide to bind to the nucleic acid molecule.

Take a picture: excite the detectable marker and collect the signal of the detectable marker.

Excision: Cleavage reagents remove the blocking group and detectable label from the first nucleotide.

Synthesis of the second strand: The third nucleotide undergoes a polymerization reaction with the second polymerase to extend the first sequencing primer, allowing the third nucleotide to bind to the nucleic acid molecule to obtain a new strand;

Second-strand polymerization: The fourth nucleotide, the third polymerase and the second sequencing primer perform a polymerization reaction to combine the second sequencing primer with the nascent strand and extend the second sequencing primer to bind the fourth nucleotide to the nascent strand. .

Take a picture: excite the detectable marker and collect the signal of the detectable marker.

Excision: A cleavage reagent removes the blocking group and detectable label of the fourth nucleotide.

Storage in the standby area: only designed for thermal insulation, and the temperature of the reagents during the current use period is kept no higher than normal temperature.

Reagent kit: The reagent kit corresponds to the reagent standby area. It only stores the reagents required for the current use trip. It does not require temperature control components, which greatly simplifies the structure and size of the reagent kit;

Reagent storage area: can be stored independently to avoid temperature changes and cross-contamination caused by frequent operation of pump liquids; can meet long-term and large-capacity storage needs; when pipetting is required, it can be connected to the reagent standby module through the pipetting module, and can be selected Whether to perform degassing and automatic cleaning of the kit; you can select designated reagents for degassing; execute the automatic cleaning kit process to automatically clean the kit, making it easy to reuse the kit, and the operator does not need to replace reagents for a long time Kits or reagents, greatly reducing operating steps and time.

During specific implementation, the following steps can be followed: S10. Preparation of amplification reagents:

S20. Liquid extraction from the reagent storage module: the pipetting module draws the reaction reagent A from the reagent storage module, and connects the selection valve through the pipetting fluid component;

S30. Degassing: When the selection valve optionally performs degassing, the selection valve switches to the degassing module, and reaction reagent A passes through the degassing component for degassing;

S40. Reaction reagent A enters the reagent standby module: After degassing, the reaction reagent A enters the reagent box of the reagent standby module through the fluid connection component. The amount of reagent pumped in is set by the control system to meet the current 1 or The amount required for several usage trips;

S50, amplification:

S60. Biochemical amplification: After the preparation of the reaction reagents required for amplification is completed, they enter the flow cell through the fluid system for the amplification reaction;

S70. Preparation of sequencing reagents: The steps performed are the same as the preparation of amplification reagents. The reagent tubes contained in the corresponding waiting area kit can be different reagent tubes or the same reagent tubes:

S80. Different tubes of amplification and sequencing reagents: When the amplification kit and sequencing reagents use different reagent tubes, the amplification reaction can be started after the amplification reagents are prepared; at the same time, the sequencing reagents are taken, pipetting from the storage area to the waiting area. Use the area to complete the preparation of test reagents;

S90. Amplification and sequencing reagents in the same tube: After the amplification reagent reaction is completed, the RMS calls the cleaning module to clean the reagent tubes, and then the pipetting module completes the movement of the sequencing reagents to the standby area kit, which can be selected Degas the sequencing reagents;

S100, sequencing:

S110. Sequencing reaction: After the preparation of sequencing reagents is completed, the reaction reagents are sequentially entered into the flow cell through the instrument fluid system to participate in the biochemical reaction, and the detection system performs signal acquisition, and the control system performs signal processing to complete the sequencing process;

S120. Automatic cleaning: After completing the current use stroke or several use strokes, the RMS calls the automatic cleaning module to perform kit cleaning. After the cleaning is completed, there is no liquid in the reagent tube and it is ready for the next pipetting.

In this embodiment, by setting up a reagent standby module, a reagent storage module, a pipetting module and a reagent using mechanism, the reagent standby module is used to temporarily store the currently required reagents; wherein, the storage space of the reagent storage module is in a normal temperature environment. , the reagent storage module is connected with the reagent standby module, the reagent storage module is used to store at least one reagent, wherein the storage space of the reagent storage module is a low-temperature environment, and the pipetting module is used to transfer all the current data stored in the reagent storage module. The required reagents are transferred to the reagent standby module, and the pipetting module is also used to transfer the currently required reagents temporarily stored in the reagent standby module to the reagent using mechanism for use by the reagent using mechanism, so that the present invention can be used when using The reagent management system has independent temperature control, cleaning and pipetting functions and can be reused.

The above are only optional embodiments of the present invention, and are not intended to limit the patent scope of the present invention. Under the inventive concept of the present invention, equivalent structural transformations can be made using the contents of the description and drawings of the present invention, or directly/indirectly. Application in other related technical fields is included in the scope of patent protection of the present invention.

Claims (10)

1. A reagent management system, comprising:

the reagent standby module is used for temporarily storing the currently required reagent; wherein, the storage space of the reagent storage module is in a normal temperature environment;

the reagent storage module is communicated with the reagent standby module and is used for storing at least one reagent, wherein the temperature in a storage space of the reagent storage module is A, and A is less than or equal to 8 ℃;

a pipetting module for transferring a currently required reagent stored in the reagent storage module to the reagent standby module; the method comprises the steps of,

a reagent use mechanism, the pipetting module further for transferring the currently desired reagent temporarily stored in the reagent standby module to the reagent use mechanism for use by the reagent use mechanism.

2. The reagent management system of claim 1, wherein a reagent standby area for storing the currently required reagent is formed in the reagent standby module, the reagent standby area being set at an ordinary temperature.

3. The reagent management system of claim 2, wherein the reagent storage module is provided with a temperature adjustment member at its outer periphery for maintaining the temperature of the reagent storage module at a.ltoreq.8℃.

4. A reagent management system according to claim 3, wherein a sealed space is formed in the reagent standby area, and the currently required reagent is temporarily stored in the sealed space by a reagent kit.

5. The reagent management system of claim 4, wherein the reagent piping system further comprises a degas treatment module for degas treating a space within the kit.

6. The reagent management system of claim 5, further comprising a washing module for washing the reagent storage module.

7. The reagent management system of claim 6, wherein the washing module is disposed in the reagent storage module.

8. The reagent management system of claim 6, wherein the reagent standby module, the pipetting module, the reagent use mechanism, the degassing processing module, the washing module, and the reagent storage module are in a pry arrangement.

9. The reagent management system of any one of claims 1 to 8, wherein the pipetting module comprises:

a horizontal member;

the vertical piece is movably arranged on the horizontal piece along the horizontal direction; the method comprises the steps of,

and the liquid transferring piece is movably arranged on the vertical piece along the vertical direction.

10. The reagent management system according to any one of claims 1 to 8, wherein the reagent standby module and the pipetting module are connected by a connecting line, and a valve is provided on the connecting line.