CN117106552A - Preparation method of probiotics powder - Google Patents
Preparation method of probiotics powder Download PDFInfo
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- CN117106552A CN117106552A CN202310922781.9A CN202310922781A CN117106552A CN 117106552 A CN117106552 A CN 117106552A CN 202310922781 A CN202310922781 A CN 202310922781A CN 117106552 A CN117106552 A CN 117106552A
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- 239000006041 probiotic Substances 0.000 title claims abstract description 46
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 46
- 239000000843 powder Substances 0.000 title claims abstract description 32
- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- 238000003756 stirring Methods 0.000 claims abstract description 55
- 238000000855 fermentation Methods 0.000 claims abstract description 37
- 230000004151 fermentation Effects 0.000 claims abstract description 37
- 230000000529 probiotic effect Effects 0.000 claims abstract description 37
- 238000007789 sealing Methods 0.000 claims abstract description 31
- 238000000034 method Methods 0.000 claims abstract description 22
- 241000894006 Bacteria Species 0.000 claims abstract description 20
- 230000001580 bacterial effect Effects 0.000 claims abstract description 19
- 238000007790 scraping Methods 0.000 claims abstract description 18
- 230000000694 effects Effects 0.000 claims abstract description 15
- 238000004140 cleaning Methods 0.000 claims abstract description 13
- 238000010438 heat treatment Methods 0.000 claims abstract description 13
- 239000007788 liquid Substances 0.000 claims abstract description 11
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 8
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 4
- 230000017525 heat dissipation Effects 0.000 claims description 24
- 230000007246 mechanism Effects 0.000 claims description 20
- 238000001816 cooling Methods 0.000 claims description 13
- 238000002156 mixing Methods 0.000 claims description 9
- 241001052560 Thallis Species 0.000 claims description 6
- 238000007599 discharging Methods 0.000 claims description 6
- 238000000227 grinding Methods 0.000 claims description 6
- 238000004806 packaging method and process Methods 0.000 claims description 6
- 239000000047 product Substances 0.000 claims description 4
- 241000883990 Flabellum Species 0.000 claims description 3
- 230000001804 emulsifying effect Effects 0.000 claims description 3
- 239000000839 emulsion Substances 0.000 claims description 3
- 238000004108 freeze drying Methods 0.000 claims description 3
- 239000002245 particle Substances 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 3
- 238000009777 vacuum freeze-drying Methods 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 239000000463 material Substances 0.000 abstract description 9
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 238000012258 culturing Methods 0.000 abstract 1
- 230000009471 action Effects 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 241000233866 Fungi Species 0.000 description 2
- 230000007413 intestinal health Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 1
- 235000017491 Bambusa tulda Nutrition 0.000 description 1
- 241001330002 Bambuseae Species 0.000 description 1
- 241000186000 Bifidobacterium Species 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 238000004026 adhesive bonding Methods 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 239000011425 bamboo Substances 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000006213 oxygenation reaction Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011897 real-time detection Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M39/00—Means for cleaning the apparatus or avoiding unwanted deposits of microorganisms
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/04—Preserving or maintaining viable microorganisms
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Abstract
The invention discloses a preparation method of probiotic bacteria powder, which comprises the following steps: s1, storing strains: storing the pure probiotic bacterial isolate in a very cold liquid nitrogen refrigerator; s2, strain fermentation: the invention relates to the technical field of preparation of probiotics, in particular to a method for culturing strains in an inner cylinder in a sterile state, wherein the inner cylinder is heated by a heating wire and fermentation broth is uniformly stirred by a stirring assembly. According to the preparation method of the probiotic bacterial powder, after bacterial powder production is discharged, screws are screwed out from between two connecting plates, a stirring rod is separated from an output shaft of a motor, then a rotating shaft is rotated to drive a threaded rod to move out of a fixed block, so that a sealing cover can be separated from an outer cylinder body, the sealing cover can directly drive a part in an inner cylinder body to move out, materials adhered to a scraping plate, a stirring assembly and the cylinder wall are conveniently removed, the cleaning is thorough, the cleaning effect is better, and pollution to subsequently produced probiotics is avoided.
Description
Technical Field
The invention relates to the technical field of preparation of probiotics, in particular to a preparation method of probiotic bacteria powder.
Background
The probiotics are active microorganisms beneficial to the host by colonizing in the human body and changing the flora composition of a certain part of the host, and the probiotic bacteria powder can maintain intestinal health balance and mainly comprises bifidobacteria and lactobacillus, and has the functions of intestinal health care, immunity enhancement, regulation and the like.
The invention has disclosed a aquatic products uses the probiotics quick expansion culture device and its application method of patent application number CN202010158056.5, it includes the stirring fermentation tank, the top of the stirring fermentation tank has sealed covers movably, the bottom of the sealed cover is embedded with the ultraviolet sterilizing lamp, there are charging tubes on the stirring fermentation tank, there are air blowers and air extractors on the stirring fermentation tank, there are filtering oxygenation devices between air blowers and stirring fermentation tank, the bottom of the stirring fermentation tank fixedly has discharging pipes, seal cover up vertically rotate and install the cylinder, the outside of the cylinder is symmetrically provided with two and scraped the wall device, the cylinder of this invention drives the scraping plate to scrape the wall to the stirring fermentation tank through the support bar, can avoid stirring the inner wall of fermentation tank from sticking;
above-mentioned patent is in the use, scrape the wall to the fermentation cylinder through fixing the wallboard of scraping on the cylinder in the fermentation cylinder, prevent that its inner wall from gluing, but scrape the wallboard when striking off the material, its surface and scrape the sword and can adhere to pile up the zymotic material, because scrape the wallboard and can't take out the clearance, be relatively poor because stickness clearance effect when follow-up washing, can adhere the zymotic material equally on cylinder and the stirring board simultaneously, fermentation cylinder inner assembly can't be demolishd in the fermentation cylinder, make follow-up fermentation cylinder and inner assembly cleaning effect relatively poor, it is longer to consume time, pollute the preparation of follow-up fungus powder easily.
Disclosure of Invention
(one) solving the technical problems
Aiming at the defects of the prior art, the invention provides a preparation method of probiotic bacteria powder, which solves the problems that the scraping plate cannot be taken out for cleaning, the viscosity cleaning effect is poor in the subsequent cleaning, and meanwhile, the inner components of the fermentation tank cannot be removed from the fermentation tank, so that the subsequent fermentation tank and the inner components are poor in cleaning effect, long in time consumption and easy to pollute the preparation of the subsequent bacteria powder.
(II) technical scheme
In order to achieve the above purpose, the invention is realized by the following technical scheme: a preparation method of probiotic bacteria powder comprises the following steps:
s1, storing strains: storing the pure probiotic bacterial isolate in a very cold liquid nitrogen refrigerator;
s2, strain fermentation: transferring strains into the inner cylinder body for cultivation in a sterile state, heating the inner cylinder body by a heating wire, uniformly stirring fermentation liquid by a stirring assembly, scraping bacterial liquid adhered on the inner cylinder body by a scraping plate, discharging the inner cylinder body after fermentation, separating a sealing cover from the outer cylinder body by a locking mechanism, directly extracting the inner cylinder body by the stirring assembly and the scraping plate in the inner cylinder body for cleaning, and rapidly cooling the fermentation temperature by a cooling mechanism;
s3, thallus concentration: centrifuging the fermentation liquor in a centrifuge, pouring supernatant, uniformly mixing the collected thalli and sterilized protection liquor in proportion, and emulsifying to obtain emulsion;
s4, freeze drying: the concentrated probiotic bacteria strain is rapidly frozen into pellet-shaped small particles, and then is subjected to vacuum freeze drying by a freeze dryer until the water activity is low;
s5, grinding and mixing: grinding and mixing the concentrated thalli in a grinder to form probiotic bacteria powder;
s6, packaging: the bacterial powder is made into various packaged probiotic products by packaging equipment.
Preferably, the fixed surface of outer barrel is connected with the cover plate, locking mechanism includes two fixed barrels, two fixed barrels are fixed respectively in the both sides of outer barrel, the equal fixedly connected with fixed block in both sides at sealed lid top, the top of fixed block runs through the cover plate and extends to the outside of cover plate, threaded hole is seted up at the top of fixed block, the back of fixed barrel inner wall one side rotates and is connected with the rotation axis, and the middle part fixedly connected with worm of rotation axis, the top of fixed barrel inner wall rotates and is connected with the montant, and the fixed surface of montant is connected with the worm wheel that meshes with the worm, the bottom of montant is through annular plate fixedly connected with and the threaded rod of screw hole looks adaptation, one side that the outer barrel was kept away from to the fixed barrel is provided with stop gear.
Preferably, the inner cylinder is fixed in the outer cylinder, the bottoms of the outer cylinder and the inner cylinder are movably connected with the top of the sealing cover, and one end of the rotating shaft, which is far away from the inner wall of the fixed cylinder, penetrates through the fixed cylinder and extends to the outside of the fixed cylinder.
Preferably, the stop gear includes two mounting panels, two one side of mounting panel respectively with the top and the bottom fixed connection of fixed cylinder one side, the upside the inserted bar has been run through at the middle part of mounting panel, the rotation axis is run through to the bottom of inserted bar and extends to the below of mounting panel, downside the inside of mounting panel passes through threaded connection with the surface of inserted bar.
Preferably, the stirring assembly comprises a motor and a stirring rod, two sides of the motor are fixedly connected with the top of the outer cylinder body through a supporting frame, one end of an output shaft of the motor is fixedly connected with a connecting plate at the top of the stirring rod, two opposite sides of the connecting plate are connected through screw threads, the bottom end of the stirring rod penetrates through the outer cylinder body and extends to the inner cylinder body, and one end of the stirring rod extending to the inner cylinder body is connected with the top of the sealing cover in a rotating mode.
Preferably, both sides of puddler all are through one side fixed connection of bracing piece and scraper blade, two the both sides of one side that the scraper blade is relative and puddler all fixedly connected with stirring vane, one side that the scraper blade kept away from the puddler and the inner wall swing joint of inner tube.
Preferably, the cooling mechanism comprises an electric telescopic rod and a fan, a temperature sensor is fixedly connected to the top of one side of the inner wall of the outer cylinder body, a heat dissipation channel is fixedly connected to one side of the outer cylinder body, the top of one side of the outer cylinder body is fixedly connected with the top of the electric telescopic rod through a frame body, and a sealing plate is fixedly connected to one end of an output shaft of the electric telescopic rod.
Preferably, the bottom of closing plate runs through the heat dissipation passageway and extends to the inside of heat dissipation passageway, the closing plate extends to the inside one side of heat dissipation passageway and the bottom swing joint of heat dissipation passageway inner wall, the surface of fan passes through support body and heat dissipation passageway's inner wall fixed connection, the one end fixedly connected with flabellum of fan output shaft.
Preferably, the heating wire is wound on the surface of the inner cylinder, and the surface of the outer cylinder is fixedly connected with supporting legs through the supporting frame.
(III) beneficial effects
The invention provides a preparation method of probiotic bacteria powder. The beneficial effects are as follows:
(1) According to the preparation method of the probiotic bacteria powder, after the bacterial powder is produced and discharged, screws are screwed out from between two connecting plates, the stirring rod is separated from the motor output shaft, then the rotating shaft is rotated to drive the worm to rotate, the worm drives the threaded rod to rotate through the worm wheel, the threaded rod drives the fixing block to move downwards when rotating, the threaded rod moves out of the fixing block through the sealing cover, so that the sealing cover can be separated from the outer cylinder body, the sealing cover can directly drive the parts in the inner cylinder body to move out, materials adhered to the scraping plate, the stirring assembly and the cylinder wall are convenient to remove, the cleaning is thorough, the cleaning effect is better, and pollution to the subsequently produced probiotics is avoided.
(2) According to the preparation method of the probiotic bacteria powder, the temperature of heating is detected through the temperature sensor, when the temperature is higher, the sealing plate is driven by the electric telescopic rod to move out of the heat dissipation channel, the inner cylinder body is subjected to heat dissipation, the fan drives the fan blade to rotate, the heat dissipation efficiency is improved, the inner cylinder body is convenient to rapidly cool, and the influence on the activity of the probiotic bacteria is avoided.
Drawings
FIG. 1 is a perspective view of the structure of the present invention;
FIG. 2 is a cross-sectional view of the structure of the present invention;
FIG. 3 is a cross-sectional view of the structure of the locking mechanism of the present invention;
FIG. 4 is a perspective view of the worm gear and worm structure of the present invention;
FIG. 5 is an enlarged view of a portion of the invention at A in FIG. 2;
FIG. 6 is a perspective view of the inner barrel structure of the present invention;
fig. 7 is a partial enlarged view of the present invention at B in fig. 2.
In the figure: 1. an inner cylinder; 2. a heating wire; 3. a stirring assembly; 31. a motor; 32. a stirring rod; 33. a connecting plate; 34. stirring blades; 4. a scraper; 5. a locking mechanism; 51. a fixed cylinder; 52. a fixed block; 53. a threaded hole; 54. a rotation shaft; 55. a worm; 56. a vertical rod; 57. a worm wheel; 58. a threaded rod; 59. a limiting mechanism; 591. a mounting plate; 592. a rod; 6. sealing cover; 7. an outer cylinder; 8. a cooling mechanism; 81. an electric telescopic rod; 82. a blower; 83. a temperature sensor; 84. a heat dissipation channel; 85. a sealing plate; 86. a fan blade; 9. a sleeve plate; 10. and (5) supporting legs.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
The invention provides a preparation method of probiotic bacteria powder, which comprises the following steps:
s1, storing strains: pure probiotic strain isolates are stored in a very cold liquid nitrogen refrigerator, so that the integrity of the strain can be maintained indefinitely;
s2, strain fermentation: transferring strains into the inner cylinder body 1 for cultivation in a sterile state, heating the inner cylinder body 1 by a heating wire 2, uniformly stirring fermentation liquid by a stirring assembly 3, scraping bacterial liquid adhered on the inner cylinder body 1 by a scraping plate 4, discharging the inner cylinder body 1 after fermentation, separating a sealing cover 6 from an outer cylinder body 7 by a locking mechanism 5, directly extracting the inner cylinder body 1 by the stirring assembly 3 and the scraping plate 4 in the inner cylinder body 1 for cleaning, and rapidly cooling fermentation temperature by a cooling mechanism 8;
s3, thallus concentration: centrifuging the fermentation liquor in a centrifuge, pouring supernatant, uniformly mixing the collected thalli and sterilized protection liquor in proportion, and emulsifying to obtain emulsion;
s4, freeze drying: the concentrated probiotic bacteria strain is rapidly frozen into pellet-shaped small particles, and then is subjected to vacuum freeze drying by a freeze dryer until the water activity is low;
s5, grinding and mixing: grinding and mixing the concentrated thalli in a grinder to form probiotic bacteria powder;
s6, packaging: the bacterial powder is made into various packaged probiotic products by packaging equipment.
Example two
Referring to fig. 1 to 6, on the basis of the first embodiment, the invention provides a preparation method of probiotic bacteria powder, a sleeve plate 9 is fixedly connected to the surface of an outer cylinder 7, a locking mechanism 5 comprises two fixed cylinders 51, the two fixed cylinders 51 are respectively fixed on two sides of the outer cylinder 7, two sides of the top of a sealing cover 6 are fixedly connected with a fixed block 52, the top of the fixed block 52 penetrates through the sleeve plate 9 and extends to the outside of the sleeve plate 9, a threaded hole 53 is formed in the top of the fixed block 52, a rotary shaft 54 is rotatably connected to the back surface of one side of the inner wall of the fixed cylinder 51, a worm 55 is fixedly connected to the middle part of the rotary shaft 54, a vertical rod 56 is rotatably connected to the top of the inner wall of the fixed cylinder 51, a threaded rod 58 which is engaged with the worm 55 is fixedly connected to the surface of the vertical rod 56 through an annular plate, one side of the fixed cylinder 51, which is far away from the outer cylinder 7, a limiting mechanism 59 is arranged on one side of the fixed cylinder 51, after the threaded rod 58 is fixedly connected with the threaded hole 53, the threaded rod 59 is fixedly connected with the threaded rod 59, the two sides of the threaded rod 59 are fixedly connected to the two sides of the outer cylinder 59 through the rotary shaft 591, the two sides of the threaded rod 591 are fixedly connected to the bottom of the mounting plate 591, the threaded plate 591 is fixedly connected to the bottom of the two side of the mounting plate 591 respectively, and the threaded plate 591 is fixedly connected to the bottom of the upper side of the threaded plate 591;
the inner cylinder 1 is fixed in the outer cylinder 7, the outer cylinder 7 is movably connected with the bottom of the inner cylinder 1 and the top of the sealing cover 6, the bottom of the sealing cover 6 is provided with a handle which is convenient for pulling the sealing cover 6 and the stirring component 3, the bottom of the sealing cover 6 is communicated with a discharging pipe, a valve is arranged in the inner cylinder, the joint of the inner cylinder 1 and the sealing cover 6 is provided with a sealing gasket through external switch control, leakage is avoided, the movable connection in the scheme is that two connecting structures are contacted but not fixed, one end of the rotating shaft 54 far away from the inner wall of the fixed cylinder 51 penetrates through the fixed cylinder 51 and extends to the outside of the fixed cylinder 51, the stirring component 3 comprises a motor 31 and a stirring rod 32, the motor 31 is controlled through an external switch and is connected with an external power supply through wires, two sides of the motor 31 are fixedly connected with the top of the outer cylinder 7 through a supporting frame, one end of the output shaft of the motor 31 is fixedly connected with a connecting plate 33 at the top of a stirring rod 32, two opposite sides of the connecting plate 33 are connected through screw threads, the connecting plate 33 and screws are arranged to facilitate the subsequent dismantling of the stirring assembly 3, the bottom end of the stirring rod 32 penetrates through the outer cylinder 7 and extends to the inner cylinder 1, one end of the stirring rod 32 extending to the inner cylinder 1 is rotationally connected with the top of the sealing cover 6, two sides of the stirring rod 32 are fixedly connected with one side of the scraping plate 4 through supporting rods, two opposite sides of the scraping plate 4 are fixedly connected with stirring blades 34 at two sides of the stirring rod 32, the stirring blades 34 are provided with a plurality of groups, stirring is more uniform, the fermentation effect is better, one side of the scraping plate 4 away from the stirring rod 32 is movably connected with the inner wall of the inner cylinder 1, the heating wire 2 is wound on the surface of the inner cylinder 1, the surface of the outer cylinder 7 is fixedly connected with supporting legs 10 through a supporting frame.
Through the setting of above-mentioned structure, the back is gone into to the probiotic raw materials to interior barrel 1, the heater strip 2 heats interior barrel 1, start motor 31, drive puddler 32 and stirring vane 34 and mix the stirring, promote fermentation effect, the puddler 32 pivoted is simultaneously through the material of bracing piece drive scraper blade 4 adhesion to interior barrel 1 inner wall scraping, avoid extravagant, after the fermentation is accomplished, fungus liquid discharge after will fermenting through the discharging pipe, unscrew the screw from between two connecting plates 33, puddler 32 and motor 31 output shaft separation, rotate inserted bar 592, screw out mounting panel 591 with the screw thread part of inserted bar 592, then take out, can rotate rotation axis 54, rotation axis 54 drives worm 55 rotation, worm 55 drives worm gear 57 and rotates, worm gear 57 drives threaded rod 58 through montant 56 and rotates, downward pulling sealed lid 6 when threaded rod 58 rotates, drive fixed block 52 downwardly moving, thereby the threaded rod 58 shifts out from fixed block 52, thereby can separate sealed lid 6 and outer barrel 7, sealed lid 6 can directly drive puddler 32 and adapting unit in interior barrel 1, be convenient for get rid of adhesion at scraper blade 4, stirring rod 32, the separation vane 34 and the back can be put into the barrel wall 5 through the outer barrel wall of a relative motion, can be thoroughly cleaned and can be put into the barrel 5 through the subsequent mechanism of the barrel wall of barrel, can be thoroughly replaced in the barrel wall is realized, can be fixed through the barrel wall is more difficult to be realized, and the barrel wall is finished through the barrel is finished, can be rotated by the barrel is finished by the back is finished by the material is directly after the material is finished.
Example III
Referring to fig. 2 and 7, on the basis of the first embodiment and the second embodiment, the cooling mechanism 8 includes an electric telescopic rod 81 and a fan 82, the electric telescopic rod 81 and the fan 82 are controlled by an external switch and are connected with an external power supply through wires, a temperature sensor 83 is fixedly connected to the top of one side of the inner wall of the outer cylinder 7, a heat dissipation channel 84 is fixedly connected to one side of the outer cylinder 7, the top of one side of the outer cylinder 7 is fixedly connected to the top of the electric telescopic rod 81 through a frame body, one end of an output shaft of the electric telescopic rod 81 is fixedly connected with a sealing plate 85, the sealing plate 85 is used for sealing the heat dissipation channel 84, the bottom of the sealing plate 85 penetrates through the heat dissipation channel 84 and extends to the inside of the heat dissipation channel 84, one side of the sealing plate 85 extending to the inside of the heat dissipation channel 84 is movably connected to the bottom of the inner wall of the heat dissipation channel 84, the surface of the fan 82 is fixedly connected to the inner wall of the heat dissipation channel 84 through a frame body, and one end of the output shaft of the fan 82 is fixedly connected with a fan blade 86.
Through the setting of above-mentioned structure, in the fermentation process, temperature sensor 83 real-time detection heating's temperature, when detecting that the temperature is higher, start electric telescopic handle 81, drive closing plate 85 and shift out heat dissipation channel 84, dispel the heat to interior barrel 1, and fan 82 drives flabellum 86 and rotate for radiating efficiency, be convenient for carry out rapid cooling to interior barrel 1, avoid influencing the activity of probiotics.
Example IV
Referring to fig. 1 to 7, the first embodiment, the second embodiment and the third embodiment are combined to obtain the present embodiment.
Through the setting of sealed lid 6 and locking mechanism 5 isotructure, can directly drive the part in the interior barrel 1 and shift out, loading and unloading are simple swift, are convenient for get rid of the material of adhesion on scraper blade 4, stirring subassembly 3 and section of thick bamboo wall, wash more thoroughly, and the cleaning effect is better, can not cause the pollution to the probiotic of follow-up production, and through the setting of cooling body 8, when detecting that interior barrel 1 temperature is higher, be convenient for carry out quick cooling to the temperature in the interior barrel 1, avoid influencing the activity of probiotic.
And all that is not described in detail in this specification is well known to those skilled in the art.
It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (9)
1. A preparation method of probiotic bacteria powder is characterized by comprising the following steps: the method comprises the following steps:
s1, storing strains: storing the pure probiotic bacterial isolate in a very cold liquid nitrogen refrigerator;
s2, strain fermentation: transferring strains to the inner cylinder body (1) for cultivation in a sterile state, heating the inner cylinder body (1) by a heating wire (2), uniformly stirring fermentation liquid by a stirring assembly (3), scraping bacterial liquid adhered on the inner cylinder body (1) by a scraping plate (4), discharging the inner cylinder body (1) after fermentation, separating a sealing cover (6) from the outer cylinder body (7) by a locking mechanism (5), directly extracting the inner cylinder body (1) by the stirring assembly (3) and the scraping plate (4) in the inner cylinder body (1), cleaning, and rapidly cooling the fermentation temperature by a cooling mechanism (8);
s3, thallus concentration: centrifuging the fermentation liquor in a centrifuge, pouring supernatant, uniformly mixing the collected thalli and sterilized protection liquor in proportion, and emulsifying to obtain emulsion;
s4, freeze drying: the concentrated probiotic bacteria strain is rapidly frozen into pellet-shaped small particles, and then is subjected to vacuum freeze drying by a freeze dryer until the water activity is low;
s5, grinding and mixing: grinding and mixing the concentrated thalli in a grinder to form probiotic bacteria powder;
s6, packaging: the bacterial powder is made into various packaged probiotic products by packaging equipment.
2. The method for preparing the probiotic bacterial powder according to claim 1, wherein the method comprises the following steps: the utility model discloses a novel automatic locking device for the outer cylinder body of the electric motor comprises an outer cylinder body (7), and is characterized in that a sleeve plate (9) is fixedly connected with the surface of the outer cylinder body (7), a locking mechanism (5) comprises two fixed cylinders (51), two fixed cylinders (51) are respectively fixed on two sides of the outer cylinder body (7), fixed blocks (52) are fixedly connected with on two sides of the top of a sealing cover (6), the top of each fixed block (52) penetrates through the sleeve plate (9) and extends to the outside of the sleeve plate (9), threaded holes (53) are formed in the top of each fixed block (52), a rotating shaft (54) is rotatably connected to the back of one side of the inner wall of the fixed cylinder (51), a worm (55) is fixedly connected to the middle part of the rotating shaft (54), a vertical rod (56) is rotatably connected to the top of the inner wall of the fixed cylinder (51), a worm wheel (57) meshed with the worm (55) is fixedly connected to the top of the vertical rod (56), the bottom of the vertical rod (56) is fixedly connected with a threaded rod (58) matched with the threaded hole (53) through an annular plate, and one side of the fixed cylinder (51) far away from the outer cylinder body (7) is provided with a limiting mechanism (59).
3. The method for preparing the probiotic bacterial powder according to claim 2, wherein the method comprises the following steps: the inner cylinder body (1) is fixed in the outer cylinder body (7), the bottoms of the outer cylinder body (7) and the inner cylinder body (1) are movably connected with the top of the sealing cover (6), and one end of the rotating shaft (54) far away from the inner wall of the fixed cylinder (51) penetrates through the fixed cylinder (51) and extends to the outside of the fixed cylinder (51).
4. The method for preparing the probiotic bacterial powder according to claim 2, wherein the method comprises the following steps: stop gear (59) include two mounting panel (591), two one side of mounting panel (591) respectively with the top and the bottom fixed connection of fixed cylinder (51) one side, the upside the middle part of mounting panel (591) is run through has inserted bar (592), rotation axis (54) are run through to the bottom of inserted bar (592) and extend to the below of mounting panel (591), downside the inside of mounting panel (591) passes through threaded connection with the surface of inserted bar (592).
5. The method for preparing the probiotic bacterial powder according to claim 1, wherein the method comprises the following steps: the stirring assembly (3) comprises a motor (31) and stirring rods (32), wherein two sides of the motor (31) are fixedly connected with the top of the outer cylinder body (7) through supporting frames, one end of an output shaft of the motor (31) is fixedly connected with a connecting plate (33) at the top of the stirring rods (32), two opposite sides of the connecting plate (33) are connected through screw threads, the bottom end of each stirring rod (32) penetrates through the outer cylinder body (7) and extends to the inner part of the inner cylinder body (1), and one end of each stirring rod (32) extending to the inner part of the inner cylinder body (1) is rotationally connected with the top of the sealing cover (6).
6. The method for preparing the probiotic bacteria powder according to claim 5, which is characterized in that: both sides of puddler (32) are all through bracing piece and one side fixed connection of scraper blade (4), two one side that scraper blade (4) are relative and both sides of puddler (32) are all fixedly connected with stirring vane (34), one side that scraper blade (4) kept away from puddler (32) and the inner wall swing joint of inner tube body (1).
7. The method for preparing the probiotic bacterial powder according to claim 1, wherein the method comprises the following steps: the cooling mechanism (8) comprises an electric telescopic rod (81) and a fan (82), a temperature sensor (83) is fixedly connected to the top of one side of the inner wall of the outer cylinder body (7), a heat dissipation channel (84) is fixedly connected to one side of the outer cylinder body (7), the top of one side of the outer cylinder body (7) is fixedly connected with the top of the electric telescopic rod (81) through a frame body, and a sealing plate (85) is fixedly connected to one end of an output shaft of the electric telescopic rod (81).
8. The method for preparing the probiotic bacterial powder according to claim 7, wherein the method comprises the following steps: the bottom of closing plate (85) runs through heat dissipation passageway (84) and extends to the inside of heat dissipation passageway (84), one side that closing plate (85) extends to the inside of heat dissipation passageway (84) and the bottom swing joint of heat dissipation passageway (84) inner wall, the surface of fan (82) is through support body and the inner wall fixed connection of heat dissipation passageway (84), the one end fixedly connected with flabellum (86) of fan (82) output shaft.
9. The method for preparing the probiotic bacterial powder according to claim 1, wherein the method comprises the following steps: the heating wire (2) is wound on the surface of the inner cylinder body (1), and the surface of the outer cylinder body (7) is fixedly connected with supporting legs (10) through a supporting frame.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310922781.9A CN117106552A (en) | 2023-07-26 | 2023-07-26 | Preparation method of probiotics powder |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202310922781.9A CN117106552A (en) | 2023-07-26 | 2023-07-26 | Preparation method of probiotics powder |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116063117A (en) * | 2022-12-01 | 2023-05-05 | 翟承松 | Fermentation device for organic fertilizer production |
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2023
- 2023-07-26 CN CN202310922781.9A patent/CN117106552A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116063117A (en) * | 2022-12-01 | 2023-05-05 | 翟承松 | Fermentation device for organic fertilizer production |
| CN116063117B (en) * | 2022-12-01 | 2025-01-21 | 龚俊宇 | A fermentation device for producing organic fertilizer |
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Application publication date: 20231124 |