CN116925884A

CN116925884A - Neural stem cell separation device and separation method

Info

Publication number: CN116925884A
Application number: CN202310890926.1A
Authority: CN
Inventors: 侯鹏; 周玲; 徐红玲; 刘先念; 杜广青
Original assignee: Shandong Benyuan Stem Cell Engineering Co ltd
Current assignee: Shandong Benyuan Stem Cell Engineering Co ltd
Priority date: 2023-07-20
Filing date: 2023-07-20
Publication date: 2023-10-24

Abstract

The application relates to the technical field of cell separation, in particular to a neural stem cell separation device and a neural stem cell separation method. According to the neural stem cell separation device and the neural stem cell separation method, the separation disc is positioned at the bottom of the supernatant through the cooperation of the separation mechanism and the clamping mechanism, so that the internal cells are prevented from being poured out by an operator, a small amount of separated neural stem cells are reduced again, and the subsequent culture of the neural stem cells is influenced.

Description

Neural stem cell separation device and separation method

Technical Field

The application relates to the technical field of cell separation, in particular to a neural stem cell separation device and a neural stem cell separation method.

Background

Neural stem cells refer to a population of cells that exist in the nervous system, have the potential to differentiate into neurons, astrocytes and oligodendrocytes, thereby being capable of producing a large amount of brain tissue, and are capable of self-renewal and sufficient to provide a large amount of brain tissue cells.

At present, when separating neural stem cells, need insert the test tube and carry out centrifugal operation in the centrifuge, cause inside cell separation, need pour or suck out the unnecessary supernatant in centrifugal test tube inside after the separation, but operating personnel is relatively easy also pour out inside cell for the reduction again of a small amount of neural stem cells after the separation influences the cultivation of follow-up neural stem cells, and traditional separation supernatant is mostly that operating personnel is manual to empty or suck out, if need the test tube of separation more, easy messenger operating personnel produces fatigue, make operating personnel's work efficiency reduce.

Disclosure of Invention

In order to solve the problems, the application provides a neural stem cell separation device and a neural stem cell separation method.

The application adopts the following technical scheme that the neural stem cell separation device comprises a base, wherein a sliding block is arranged on the upper surface of the lower end of the base in a sliding way, a liquid receiver is fixedly connected on the sliding block, a motor case is fixedly arranged on the outer wall of one side of the base through bolts, a rotating shaft is rotatably arranged between two opposite inner walls of the base, a rotating table is fixedly connected on the outer wall of the rotating shaft, and a separation mechanism, a leakage-proof mechanism and a clamping mechanism are arranged at the upper end of the rotating table;

the separating mechanism comprises a first electric push rod fixedly connected to the center of the upper surface of the rotary table, a connecting frame is fixedly connected to the upper end of the first electric push rod, a fixing pipe is fixedly connected to the lower end of the outer side of the connecting frame, a fixing frame is fixedly connected to the lower end of the fixing frame, a connecting ring is fixedly connected to the lower end of the fixing frame, an inserting rod is slidably connected to the inner cavity of the vertical end of the fixing frame, the lower end of the inserting rod is movably inserted to the upper surface of the connecting ring, the lower end of the inserting rod extends to the lower end of the connecting ring, a first trapezoidal rod is movably inserted into the inner cavity of the horizontal end of the fixing frame, a second trapezoidal rod is slidably arranged in the fixing pipe, first trapezoidal blocks are arranged on the two sides of the lower end of the second trapezoidal rod in the separating disc, through holes are formed in the two opposite sides of the upper end of the separating disc, one end of the first trapezoidal block is located in the through holes, two opposite side walls of the first trapezoidal block are fixedly connected with connecting blocks, the connecting blocks are slidably arranged in the separating disc, the connecting blocks are close to the first trapezoidal blocks and the bottom of the separating disc are flush with the first trapezoidal rod, and the first end of the separating disc is connected with the first spring.

As a further description of the above technical solution: the outer wall of the upper end of the rotary table is provided with an observation notch, and a test tube is placed inside the upper end of the rotary table.

As a further description of the above technical solution: the leakage-proof mechanism comprises sleeve blocks fixedly connected to the upper end of the rotary table, plug blocks are movably inserted into inner cavities of the upper ends of the sleeve blocks, two sleeve blocks are arranged, annular plates are fixedly connected to the upper ends of the plug blocks, conical blocks are fixedly connected to the outer walls of the annular plates, the conical blocks are movably sleeved on the outer walls of the fixed pipes, second springs are fixedly connected between the two sides of the lower ends of the plug blocks and the sleeve blocks, limit rods are movably inserted into one side walls of the sleeve blocks, and third springs are fixedly connected between the inner walls of the outer ends of the limit rods and the outer walls of the sleeve blocks.

As a further description of the above technical solution: the center of the lower end of the inserting block is fixedly connected with a third trapezoid rod, the upper end of the third trapezoid rod is movably inserted into the lower end of the sleeve block, the lower end of the third trapezoid rod is movably inserted into the rotary table, a trapezoid plate is arranged in the rotary table in a sliding mode, one side of the trapezoid plate is positioned in the rotary table, a fourth trapezoid rod is movably inserted into the rotary table, one end of the fourth trapezoid rod is positioned outside the rotary table, a stop block is fixedly connected to the inner wall of the vertical end of the base close to the fourth trapezoid rod, and the stop block and the fourth trapezoid rod are symmetrically arranged about the rotary shaft.

As a further description of the above technical solution: the clamping mechanism comprises a second electric push rod fixedly connected in the rotary table, an annular disc is fixedly connected to the upper end of the second electric push rod, moving blocks are movably inserted into the two sides of the test tube at the upper end of the connecting ring, a second trapezoid block is arranged in the annular disc in a sliding mode, one end of the second trapezoid block, which is close to the first electric push rod, extends to the outer side of the annular disc, a stop lever is arranged at one end, which is close to the outer side of the annular disc, of the second trapezoid block, an arc-shaped clamping block is fixedly connected to the inner wall of the stop lever, the arc-shaped clamping block is movably inserted into the rotary table, and a fourth spring is fixedly connected between the side wall, which is far away from the test tube, of the arc-shaped clamping block and the rotary table.

As a further description of the above technical solution: the test tube, the fixed tube and the conical block are all provided with a plurality of test tubes, and the fixed tube and the conical block are all distributed in annular equidistance.

As a further description of the above technical solution: rubber rings are adhered to the inner wall of the conical block and the outer wall of the fixed pipe.

As a further description of the above technical solution: the guiding gutter has been seted up to toper piece lower extreme lateral wall, toper piece upper end diameter, the diameter of separating disc are the same with the diameter of test tube.

In addition, the application adopts the following technical scheme, and the separation method of the neural stem cell separation device comprises the following steps:

s1, centrifuging at room temperature, placing a test tube in a notch at the upper end of a rotary table, positioning a separation disc to the bottom of supernatant through the cooperation of a separation mechanism and a clamping mechanism, and clamping and fixing the test tube by an arc-shaped clamping block in the clamping mechanism;

s2, starting a motor in the motor box, rotating the turntable to a position with a test tube mouth facing downwards through a rotating shaft, automatically opening a leakage-proof mechanism, and pouring supernatant into the liquid receiver;

s3, turning the turntable to an initial position, then moving the first electric push rod upwards, opening the leakage-proof mechanism, and taking down the test tube;

s4, finally, the cells are resuspended in a serum-free N2 culture solution containing 10% DMSO and proper growth factors, gently blown by a suction tube to make the cells uniform, and then sub-packaged in sterile freezer tubes.

In the technical scheme, the neural stem cell separation device and the neural stem cell separation method provided by the application have the advantages that the separation disc is positioned at the bottom of the supernatant by the cooperation of the separation mechanism and the clamping mechanism, so that the internal cells are prevented from being poured out by an operator, the separated few neural stem cells are reduced again, and the subsequent culture of the neural stem cells is influenced.

Further, simultaneously when the go-between moves down the in-process, after the go-between extrudees the movable block, the movable block pushes down, and the second trapezoidal piece of both sides moves to the inboard, makes pin and arc clamp splice to the inboard simultaneously and moves, and the arc clamp splice presss from both sides the test tube clamp, prevents that the test tube from taking place to rock, and the device can replace the manual work to get the operation of supernatant, has improved the efficiency of staff's separation neural stem cell.

Drawings

The application is further explained below with reference to the drawings and examples:

fig. 1 is a schematic structural diagram of a neural stem cell separation device and a neural stem cell separation method according to an embodiment of the present application;

fig. 2 is a schematic structural diagram II of a neural stem cell separation device and a neural stem cell separation method according to an embodiment of the present application;

FIG. 3 is a schematic structural diagram of a separation mechanism according to an embodiment of the present application;

FIG. 4 is a cross-sectional view of a mount according to an embodiment of the present application;

FIG. 5 is a transverse cross-sectional view of a separator plate provided in an embodiment of the present application;

fig. 6 is a schematic structural diagram of a clamping mechanism according to an embodiment of the present application;

FIG. 7 is an enlarged view of FIG. 2 at A;

fig. 8 is an enlarged view at B in fig. 6.

In the figure: 1. a base; 2. a turntable; 3. a motor case; 4. a slide block; 5. a liquid collector; 6. a separation mechanism; 61. a connecting frame; 62. a fixing frame; 63. a fixed tube; 64. a separation disc; 65. a rod; 66. a first ladder bar; 67. a second trapezoidal bar; 68. a first trapezoidal block; 69. a through hole; 610. connecting blocks; 611. a first spring; 612. a first electric push rod; 613. a connecting ring; 7. a leakage prevention mechanism; 71. an annular plate; 72. a conical block; 73. inserting blocks; 74. sleeving blocks; 75. a second spring; 76. a third ladder bar; 77. a trapezoidal plate; 78. a fourth trapezoidal bar; 79. a limit rod; 710. a third spring; 711. a stop block; 8. a clamping mechanism; 81. an annular disc; 82. a moving block; 83. a second electric push rod; 84. a second trapezoidal block; 85. a stop lever; 86. arc clamping blocks; 87. a fourth spring; 9. a rotating shaft; 10. a test tube; 11. the notch is observed.

Detailed Description

The application is further described with reference to the following detailed drawings in order to make the technical means, the creation characteristics, the achievement of the purpose and the effect of the implementation of the application easy to understand. It should be noted that, without conflict, the embodiments of the present application and features of the embodiments may be combined with each other.

Referring to fig. 1 to 8, the embodiment of the present application provides a technical solution: the nerve stem cell separation device comprises a base 1, wherein a sliding block 4 is arranged on the upper surface of the lower end of the base 1 in a sliding manner, a liquid receiver 5 is fixedly connected to the sliding block 4, a motor box 3 is fixedly arranged on the outer wall of one side of the base 1 through bolts, a rotating shaft 9 is rotatably arranged between two opposite inner walls of the base 1, a rotating table 2 is fixedly connected to the outer wall of the rotating shaft 9, and a separation mechanism 6, a leakage-proof mechanism 7 and a clamping mechanism 8 are arranged at the upper end of the rotating table 2;

the separating mechanism 6 comprises a first electric push rod 612 fixedly connected to the center of the upper surface of the turntable 2, a connecting frame 61 is fixedly connected to the upper end of the first electric push rod 612, a fixing tube 63 is fixedly connected to the lower end of the outer side of the connecting frame 61, the fixing tube 63 is far away from the outer wall of the first electric push rod 612, a fixing frame 62 is fixedly connected to a connecting ring 613, an inserting rod 65 is slidably connected to the inner cavity of the vertical end of the fixing frame 62, the lower end of the inserting rod 65 is movably inserted into the upper surface of the connecting ring 613, the lower end of the inserting rod 65 extends to the lower end of the connecting ring 613, a first trapezoidal rod 66 is movably inserted into the inner cavity of the horizontal end of the fixing frame 62, a second trapezoidal rod 67 is slidably arranged in the fixing tube 63, two sides of the lower end of the second trapezoidal rod 67 are both sides of the second trapezoidal rod 67 are arranged in the separating disc 64, a first trapezoidal block 68 is slidably arranged in the separating disc 64, two opposite sides of the upper end of the separating disc 64 are both sides of the separating disc 64 are respectively provided with a through hole 69, one end of the first trapezoidal block 68 far away from the second trapezoidal rod 67 is located in the through hole 69, two opposite side walls of the first trapezoidal block 68 are fixedly connected to the two opposite side walls of the connecting blocks 67, the two opposite side walls of the first trapezoidal block 68 are respectively provided with a second trapezoidal block 67, and the connecting block 67 is arranged in the separating disc 610 is close to the separating disc 64, and the bottom 610 is arranged in the separating disc 610 is close to the connecting disc 610, and the upper end is close to the connecting disc 610 is arranged in the separating disc 610.

Specifically, when the supernatant and the neural stem cells need to be separated, the centrifuged test tube 10 is placed in the turntable 2, then the clamping mechanism 8 is adjusted according to the position of the supernatant in the test tube, then the first electric push rod 612 is started, the connecting frame 61, the fixing frame 62 and the fixing tube 63 are moved downwards, the inserting rod 65 is moved upwards, then the second trapezoidal rod 67 is moved to two sides through the cooperation of the inserting rod 65, the first trapezoidal rod 66, the second trapezoidal rod 67 and the first trapezoidal block 68, after the separating disc 64 is moved to the bottom of the supernatant, the through hole 69 is also blocked, then the test tube 10 is blocked by the leakage-proof mechanism 7, the clamping mechanism 8 can clamp the test tube 10, then the motor in the motor box 3 is started, the turntable 2 is rotated to the position with the mouth of the test tube 10 downwards through the rotating shaft 9, then the leakage-proof mechanism 7 can be opened automatically, the supernatant is poured into the liquid collector 5, finally the turntable 2 is turned to the initial position, then the first electric push rod 612 is moved upwards, and the leakage-proof mechanism 7 is opened, and the test tube 10 is removed.

In still another embodiment provided by the application, an observation notch 11 is formed in the outer wall of the upper end of the turntable 2, and a test tube 10 is placed in the upper end of the turntable 2.

Specifically, the bottom most position of the supernatant can be observed clearly by observing the notch 11, and then the separating tray 64 can be moved to the bottom end of the supernatant by adjusting the holding mechanism 8 to the corresponding position.

In still another embodiment of the present application, the leakage preventing mechanism 7 includes a sleeve block 74 fixedly connected to the upper end of the turntable 2, and the inner cavity of the upper end of the sleeve block 74 is movably inserted with an insert block 73, two sleeve blocks 74 are provided, the upper end of the insert block 73 is fixedly connected with an annular plate 71, the outer wall of the annular plate 71 is fixedly connected with a conical block 72, the conical block 72 is movably sleeved on the outer wall of the fixed pipe 63, a second spring 75 is fixedly connected between two sides of the lower end of the insert block 73 and the sleeve block 74, a limiting rod 79 is movably inserted between the sleeve block 74 and one side wall of the insert block 73, and a third spring 710 is fixedly connected between the inner wall of the outer end of the limiting rod 79 and the outer wall of the sleeve block 74.

Specifically, when the test tube 10 is placed in the turntable 2, the limiting rod 79 is pulled outwards, then the insert block 73 drives the annular plate 71 and the conical block 72 to move downwards to the upper end of the separating disc 64, and then the first electric push rod 612 is started to move the separating disc 64 downwards into the test tube 10, so that the conical block 72 moves downwards to the mouth of the test tube 10 due to the elasticity of the second spring 75, and the mouth of the test tube 10 is blocked.

In still another embodiment of the present application, a third trapezoid bar 76 is fixedly connected to the center of the lower end of the insert block 73, the upper end of the third trapezoid bar 76 is movably inserted into the lower end of the sleeve block 74, the lower end of the third trapezoid bar 76 is movably inserted into the turntable 2, a trapezoid plate 77 is slidably disposed on the lower side of the third trapezoid bar 76 in the turntable 2, one side of the trapezoid plate 77 is movably inserted into a fourth trapezoid bar 78 in the turntable 2, one end of the fourth trapezoid bar 78 is located outside the turntable 2, a stop block 711 is fixedly connected to the inner wall of the base 1 near the vertical end of the fourth trapezoid bar 78, and the stop block 711 and the fourth trapezoid bar 78 are symmetrically disposed about the rotating shaft 9.

Specifically, rotating the shaft 9, the stopper 711 will press the fourth trapezoidal bar 78 to move inward, the fourth trapezoidal bar 78 presses the trapezoidal plate 77 to move toward the third trapezoidal bar 76, then the trapezoidal plate 77 presses the third trapezoidal bar 76, and the third trapezoidal bar 76 moves upward, so that the tapered block 72 moves upward and leaves the mouth of the test tube 10, and the supernatant can be poured.

In still another embodiment of the present application, the clamping mechanism 8 includes a second electric push rod 83 fixedly connected to the turntable 2, an annular disc 81 is fixedly connected to the upper end of the second electric push rod 83, moving blocks 82 are movably inserted into the upper ends of the connecting rings 613 located at two sides of the test tube 10, a second trapezoid block 84 is slidably disposed on the lower side of the moving blocks 82 located in the annular disc 81, one end of the second trapezoid block 84, which is close to the first electric push rod 612, extends to the outer side of the annular disc 81, a stop lever 85 is disposed on one side, which is close to the test tube 10, of the end, which extends to the outer side of the annular disc 81, of the second trapezoid block 84, an arc-shaped clamping block 86 is fixedly connected to the inner wall of the stop lever 85, the arc-shaped clamping block 86 is movably inserted into the turntable 2, and a fourth spring 87 is fixedly connected between the side wall, which is far away from the test tube 10, of the arc-shaped clamping block 86 and the turntable 2.

Specifically, the bottom-most position of the supernatant can be observed through the observation notch 11, and then the annular disk 81 is adjusted to the corresponding position, when the connecting ring 613 moves downward, and after the connecting ring 613 presses the moving block 82, the moving block 82 presses down, the second trapezoidal blocks 84 on both sides move inward, and simultaneously the stop lever 85 and the arc-shaped clamping block 86 move inward, and then the arc-shaped clamping block 86 clamps the test tube 10.

In yet another embodiment provided by the present application, the test tube 10, the fixed tube 63 and the tapered block 72 are all provided with a plurality of test tubes 10, the fixed tube 63 and the tapered block 72 are all distributed in an annular equidistant manner.

In yet another embodiment of the present application, rubber rings are bonded to both the inner wall of the tapered block 72 and the outer wall of the fixed tube 63.

Specifically, the supernatant fluid does not flow out from the contact gap between the inner wall of the tapered block 72 and the outer wall of the fixed pipe 63 during the rotation of the turntable 2.

In yet another embodiment of the present application, the side wall of the lower end of the conical block 72 is provided with a flow guide groove, and the diameter of the upper end of the conical block 72 and the diameter of the separating disc 64 are the same as those of the test tube 10.

Working principle: when the supernatant is required to be separated from the neural stem cells, the test tube 10 is firstly centrifuged and then placed in the turntable 2, the bottommost position of the supernatant can be observed through the observation notch 11, then the annular disc 81 is adjusted to the corresponding position, after the test tube 10 is placed in the turntable 2, the limiting rod 79 is pulled outwards, the annular plate 71 and the conical block 72 are driven to move downwards to the upper end of the separating disc 64 by the inserting block 73, then the separating disc 64 is moved downwards into the test tube 10 by starting the first electric push rod 612, the conical block 72 is moved downwards to the opening of the test tube 10 due to the elasticity of the second spring 75, the test tube 10 is blocked, then the first electric push rod 612 is started, the connecting frame 61, the fixing frame 62 and the fixing tube 63 are moved downwards, the inserting rod 65 is moved upwards, and then the inserting rod 65, the first trapezoidal rod 66, the second trapezoidal rod 67 and the first trapezoidal block 68 are matched, the second trapezoid bar 67 is moved to both sides, after the separating disc 64 is moved to the bottom of the supernatant, the through hole 69 is also blocked, then the conical block 72 blocks the mouth of the test tube 10, when the connecting ring 613 is moved downwards, after the connecting ring 613 is pressed to the moving block 82, the moving block 82 is pressed down, the second trapezoid block 84 on both sides is moved inwards, simultaneously the stop bar 85 and the arc-shaped clamping block 86 are moved inwards, then the arc-shaped clamping block 86 clamps the test tube 10, then the motor in the motor case 3 is started, the turntable 2 is rotated to a position with the mouth of the test tube 10 facing downwards through the rotating shaft 9, the stop 711 is pressed to move the fourth trapezoid bar 78 inwards, the fourth trapezoid bar 78 is pressed to move the trapezoid plate 77 to the third trapezoid bar 76, then the trapezoid plate 77 is pressed to the third trapezoid bar 76 is moved upwards, the conical block 72 is moved upwards and leaves the mouth of the test tube 10, the supernatant can be poured, and finally the turntable 2 is turned to the initial position, after which the first electric push rod 612 is moved up, the conical block 72 is moved up, and the test tube 10 is removed.

A method of separating a neural stem cell separation device, comprising the steps of:

s1, centrifuging at room temperature, placing a test tube 10 in a notch at the upper end of a turntable 2, positioning a separation disc 64 to the bottom of supernatant through the cooperation of a separation mechanism 6 and a clamping mechanism 8, and clamping and fixing the test tube 10 by an arc-shaped clamping block 86 in the clamping mechanism 8;

s2, starting a motor in the motor case 3, rotating the turntable 2 to a position with a downward opening of the test tube 10 through the rotating shaft 9, automatically opening the leakage-proof mechanism 7, and pouring supernatant into the liquid receiver 5;

s3, turning the turntable 2 to an initial position, then moving the first electric push rod 612 upwards, opening the leakage-proof mechanism 7, and taking down the test tube 10;

The foregoing has shown and described the basic principles, principal features and advantages of the application. It will be understood by those skilled in the art that the present application is not limited to the foregoing embodiments, and that the foregoing embodiments and description are merely illustrative of the principles of this application, and various changes and modifications may be made without departing from the spirit and scope of the application, and these changes and modifications fall within the scope of the application as hereinafter claimed. The scope of the application is defined by the appended claims and equivalents thereof.

Claims

1. A neural stem cell separation device, comprising a base (1), characterized in that: the upper surface of the lower end of the base (1) is provided with a sliding block (4) in a sliding manner, the sliding block (4) is fixedly connected with a liquid receiver (5), a motor box (3) is fixedly connected with the outer wall of one side of the base (1) through bolts, a rotating shaft (9) is rotatably arranged between two opposite inner walls of the base (1), the outer wall of the rotating shaft (9) is fixedly connected with a rotary table (2), and the upper end of the rotary table (2) is provided with a separating mechanism (6), a leakage-proof mechanism (7) and a clamping mechanism (8);

the separating mechanism (6) comprises a first electric push rod (612) fixedly connected to the center of the upper surface of the rotary table (2), a connecting frame (61) is fixedly connected to the upper end of the first electric push rod (612), a fixing tube (63) is fixedly connected to the lower end of the outer side of the connecting frame (61), a fixing frame (62) is fixedly connected to the outer wall of the fixing tube (63) far away from the first electric push rod (612), a connecting ring (613) is fixedly connected to the lower end of the fixing frame (62), a inserting rod (65) is slidably connected to the inner cavity of the vertical end of the fixing frame (62), the lower end of the inserting rod (65) is movably inserted into the upper surface of the connecting ring (613), the lower end of the inserting rod (65) extends to the lower end of the connecting ring (613), a first trapezoidal rod (66) is movably inserted into the inner cavity of the horizontal end of the fixing frame (62), a second trapezoidal rod (67) is arranged in the inner side of the fixing tube (63), the lower end of the second trapezoidal rod (67) is positioned in the separating disc (64), two sides of the lower end of the second trapezoidal rod (67) are positioned in the separating disc (68), first trapezoidal rod (68) are both arranged in the separating disc (68), two opposite sides of the first trapezoidal rod (68) are positioned in the separating disc (68), the two opposite side walls of the first trapezoid block (68) are fixedly connected with connecting blocks (610), the connecting blocks (610) are slidably arranged in the separating disc (64), a first spring (611) is fixedly connected between one end, close to the second trapezoid rod (67), of the connecting block (610) and the separating disc (64), and the connecting ring (613) is flush with the bottom of the separating disc (64).

2. The neural stem cell separation device of claim 1, wherein: an observation notch (11) is formed in the outer wall of the upper end of the rotary table (2), and a test tube (10) is placed in the upper end of the rotary table (2).

3. The neural stem cell separation device of claim 2, wherein: the anti-leakage mechanism (7) comprises a sleeve block (74) fixedly connected to the upper end of the rotary table (2), an inserting block (73) is movably inserted into an inner cavity of the upper end of the sleeve block (74), two sleeve blocks (74) are arranged, an annular plate (71) is fixedly connected to the upper end of each inserting block (73), a conical block (72) is fixedly connected to the outer wall of each annular plate (71), the conical blocks (72) are movably sleeved on the outer wall of each fixing tube (63), a second spring (75) is fixedly connected between two sides of the lower end of each inserting block (73) and the corresponding sleeve block (74), a limiting rod (79) is movably inserted into one side wall of each sleeve block (73), and a third spring (710) is fixedly connected between the inner wall of the outer end of each limiting rod (79) and the outer wall of each sleeve block (74).

4. A neural stem cell separation device according to claim 3, wherein: the novel rotary turntable is characterized in that a third trapezoid rod (76) is fixedly connected to the center of the lower end of the inserting block (73), the upper end of the third trapezoid rod (76) is movably inserted into the lower end of the sleeve block (74), the lower end of the third trapezoid rod (76) is movably inserted into the turntable (2), a trapezoid plate (77) is slidably arranged in the turntable (2) at the lower side of the third trapezoid rod (76), a fourth trapezoid rod (78) is movably inserted into the turntable (2) at one side of the trapezoid plate (77), one end of the fourth trapezoid rod (78) is positioned outside the turntable (2), a stop block (711) is fixedly connected to the inner wall of the vertical end of the base (1) close to the fourth trapezoid rod (78), and the stop block (711) and the fourth trapezoid rod (78) are symmetrically arranged about the rotating shaft (9).

5. The neural stem cell separation device of claim 2, wherein: the clamping mechanism (8) comprises a second electric push rod (83) fixedly connected in the rotary table (2), an annular disc (81) is fixedly connected to the upper end of the second electric push rod (83), moving blocks (82) are movably inserted into the two sides of the test tube (10) at the upper end of the connecting ring (613), a second trapezoid block (84) is slidably arranged in the annular disc (81) at the lower side of the moving blocks (82), one end, close to the first electric push rod (612), of the second trapezoid block (84) extends to the outer side of the annular disc (81), a stop lever (85) is arranged at one end, close to the test tube (10), of the outer side of the second trapezoid block (84), an arc-shaped clamping block (86) is fixedly connected to the inner wall of the stop lever (85), and a fourth spring (87) is movably inserted into the rotary table (2) in an inserting mode, and the side wall, far from the test tube (10), of the arc-shaped clamping block (86) is fixedly connected with the rotary table (2).

6. A neural stem cell separation device according to claim 3, wherein: the test tube (10), the fixed tube (63) and the conical block (72) are all provided with a plurality of test tubes (10), and the fixed tube (63) and the conical block (72) are all distributed in annular equidistance.

7. A neural stem cell separation device according to claim 3, wherein: rubber rings are adhered to the inner wall of the conical block (72) and the outer wall of the fixed pipe (63).

8. A neural stem cell separation device according to claim 3, wherein: the guiding gutter has been seted up to toper piece (72) lower extreme lateral wall, toper piece (72) upper end diameter, the diameter of separation dish (64) are the same with the diameter of test tube (10).

9. The method according to any one of claims 1 to 8, wherein: the method comprises the following steps:

s1, centrifuging at room temperature, placing a test tube (10) in a notch at the upper end of a turntable (2), positioning a separation disc (64) to the bottom of supernatant through the cooperation of a separation mechanism (6) and a clamping mechanism (8), and clamping and fixing the test tube (10) by an arc-shaped clamping block (86) in the clamping mechanism (8);

s2, starting a motor in the motor case (3), rotating the rotary table (2) to a position with a downward mouth of the test tube (10) through the rotary shaft (9), automatically opening the leakage-proof mechanism (7), and pouring supernatant into the liquid receiver (5);

s3, turning the turntable (2) to an initial position, then moving the first electric push rod (612) upwards, opening the leakage-proof mechanism (7), and taking down the test tube (10);