CN116808138A - Natto composition for regulating intestines and stomach and reducing blood fat and application thereof - Google Patents
Natto composition for regulating intestines and stomach and reducing blood fat and application thereof Download PDFInfo
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Abstract
The application relates to a natto composition for regulating intestines and stomach and reducing blood fat and application thereof, and relates to the technical field of preparation of blood fat reducing medicines. According to the application, the natto, the rhizoma polygonati extract and the radix puerariae extract are combined for use for the first time, and the natto composition with the functions of regulating intestines and stomach and reducing blood fat is obtained through the mutual cooperation and the combined action, and the natto composition has an excellent blood fat reducing effect, can provide more medicine choices for patients with hyperlipidemia, and widens the application range of rhizoma polygonati, radix puerariae and the like.
Description
Technical Field
The application relates to the technical field of preparation of hypolipidemic drugs, in particular to a natto composition for regulating intestines and stomach and reducing blood lipid and application thereof.
Background
Along with the change of dietary structure and the acceleration of life rhythm of modern people, intestinal flora imbalance such as diarrhea and constipation and metabolic related diseases such as dyslipidemia appear, and the normal life and physical health of people are seriously affected. The continuous increase of the population suffering from hyperlipidemia brings great social attention, and hyperlipidemia refers to the fact that the blood lipid level such as serum total cholesterol TC level, serum triglyceride TG level and the like in a human body is too high, so that blood is thick, deposits on the wall of a blood vessel, forms thrombus seriously and affects the health of the human body seriously. Therefore, the development of more hypolipidemic agents is becoming an important point of current research.
The natto is a soybean product prepared by fermenting soybeans through bacillus natto (bacillus subtilis), has viscosity, smells relatively bad, tastes slightly sweet, not only maintains the nutritive value of the soybeans, is rich in vitamin K2 and improves the digestibility of proteins, but also generates various physiologically active substances in the fermentation process, and has the health-care effects of dissolving in-vivo fibrin and other regulating physiological functions.
Rhizoma Polygonati is dry rhizome of Polygonatum kingianum, polygonatum sibiricum or Polygonatum cyrtonema of Liliaceae, and has effects of invigorating qi, nourishing yin, invigorating spleen, moistening lung, and invigorating kidney.
The radix Puerariae is dry root of Pueraria lobata Ohwi of Leguminosae, and has effects of relieving fever, promoting salivation, and relieving diarrhea.
At present, no report on the combined application of natto, rhizoma polygonati and radix puerariae as raw materials in the preparation of hypolipidemic drugs is seen in the prior art.
Disclosure of Invention
In order to solve the problems, the application provides the natto composition for regulating intestines and stomach and reducing blood fat and the application thereof, and the natto composition for regulating intestines and stomach and reducing blood fat is obtained by combining the natto, the rhizoma polygonati extract and the radix puerariae extract for the first time, so that more medicine choices can be provided for patients with hyperlipidemia, and the application range of rhizoma polygonati, radix puerariae and the like is widened.
In a first aspect, the application provides a natto composition for regulating intestines and stomach and reducing blood fat, which comprises natto, rhizoma polygonati extract and radix puerariae extract.
Further, the natto composition for conditioning intestines and stomach and reducing blood fat comprises the following components in parts by weight:
25-45 parts of natto, 8-15 parts of rhizoma polygonati extract and 5-10 parts of radix puerariae extract.
Further, the natto composition for conditioning intestines and stomach and reducing blood fat further comprises, in parts by weight: 1-3 parts of licorice extract.
Further, the natto composition for conditioning intestines and stomach and reducing blood fat comprises the following components in parts by weight:
37 parts of natto, 13 parts of rhizoma polygonati extract and 6 parts of radix puerariae extract.
Further, the preparation method of the rhizoma polygonati extract comprises the following steps:
adding the crushed rhizoma polygonati skin into ethanol with the volume fraction of 75-85% and the weight of 8-15 times of the crushed rhizoma polygonati skin at the temperature of 80-90 ℃ for reflux extraction for 1-3 hours, collecting an extracting solution, and concentrating under reduced pressure to obtain an extract;
loading the extract on a polyamide column, eluting with ethanol with the volume fraction of 15-20% which is 3-5 times of the column volume; eluting with ethanol with the volume fraction of 40-45% and the volume fraction of 6-10 times of the column volume, collecting ethanol eluate with the volume fraction of 40-45%, concentrating and drying to obtain the rhizoma polygonati extract.
Further, the preparation method of the kudzuvine root extract comprises the following steps:
adding crushed radix Puerariae into water, performing reduced pressure distillation, stopping reduced pressure distillation when the volume of distillate is half of the volume of the initial water adding amount, collecting reduced pressure distillation extractive solution, concentrating and drying to obtain radix Puerariae extract;
wherein, the working parameters of the reduced pressure distillation extraction comprise: the temperature is 68-72 ℃, the pressure is 50-55 Kpa, and the extraction times are 1-3 times.
Further, the licorice extract is a licorice water extract.
In a second aspect, the application provides the use of the natto composition for regulating stomach and reducing blood fat in the first aspect for preparing a medicament for reducing blood fat and/or a health care product.
In a third aspect, the present application provides a hypolipidemic drug, wherein the active ingredient of the hypolipidemic drug is the natto composition for conditioning intestines and stomach to reduce blood lipid according to any one of the first aspects.
Further, the dosage form of the hypolipidemic drug comprises at least one of granules, capsules, tablets, medicinal granules, oral liquid and powder.
Compared with the prior art, the technical scheme provided by the embodiment of the application has at least the following advantages:
the embodiment of the application provides a natto composition for regulating intestines and stomach and reducing blood fat and application thereof.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the application and together with the description, serve to explain the principles of the application.
In order to more clearly illustrate the embodiments of the application or the technical solutions of the prior art, the drawings which are used in the description of the embodiments or the prior art will be briefly described, and it will be obvious to a person skilled in the art that other drawings can be obtained from these drawings without inventive effort.
Fig. 1 is a schematic flow chart of a preparation method of a natto composition for regulating intestines and stomach and reducing blood lipid in an embodiment of the application.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present application more apparent, the technical solutions of the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present application, and it is apparent that the described embodiments are some embodiments of the present application, but not all embodiments of the present application. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.
Unless otherwise specifically indicated, the various raw materials, reagents, instruments, equipment and the like used in the present application are commercially available or may be prepared by existing methods.
In a first aspect, the application provides a natto composition for regulating intestines and stomach and reducing blood fat, which comprises natto, rhizoma polygonati extract and radix puerariae extract.
According to the application, the natto, the rhizoma polygonati extract and the radix puerariae extract are combined for use for the first time, and the natto composition with the functions of regulating intestines and stomach and reducing blood fat is obtained through the mutual cooperation and the combined action, and the natto composition has an excellent blood fat reducing effect, can provide more medicine choices for patients with hyperlipidemia, and widens the application range of rhizoma polygonati, radix puerariae and the like.
The natto adopted in the application can be a commercial product or can be prepared by self according to the existing natto preparation process. Specifically, the natto can be prepared by the following method: soaking soybeans in water for 12-24 hours; then pressurizing the soaked soybeans (under 2-10 atmospheric pressures) and steaming for 1-3 hours, cooling the steamed soybeans to room temperature, and uniformly spraying bacillus natto liquid (a commercially available product); controlling the temperature of natto at 35-45 ℃ and the relative humidity at 50-90%, fermenting for 12-14 h; and (3) putting the fermented natto into a refrigerator at 4 ℃, taking out, freeze-drying, crushing, and sieving with a 50-60-mesh sieve to obtain the natto.
The extract of the flavescent sophora root is an extract obtained by taking dried rhizome of Polygonatum sibiricum Red as a medicine.
The radix puerariae extract is an extract which is used as a medicine by dried roots of radix puerariae.
In some embodiments, the natto composition for regulating gastrointestinal blood lipid may be composed of natto, rhizoma Polygonati extract and radix Puerariae extract only.
As an implementation mode of the embodiment of the application, the natto composition for conditioning intestines and stomach and reducing blood fat comprises the following components in parts by weight:
25-45 parts of natto, 8-15 parts of rhizoma polygonati extract and 5-10 parts of radix puerariae extract.
The applicant further researches find that the natto, the rhizoma polygonati extract and the radix puerariae extract with the above dosage proportion have better blood fat reducing effect.
In some specific embodiments, the parts by weight of the natto may be 25 parts, 26 parts, 27 parts, 28 parts, 29 parts, 30 parts, 31 parts, 32 parts, 33 parts, 34 parts, 35 parts, 36 parts, 37 parts, 38 parts, 39 parts, 40 parts, 41 parts, 42 parts, 43 parts, 44 parts, 45 parts, etc.
In some embodiments, the parts by weight of the sealwort extract may be 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, etc.
In some embodiments, the parts by weight of the kudzuvine root extract may be 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, etc.
Preferably, the natto composition for conditioning intestines and stomach and reducing blood fat comprises the following components in parts by weight:
37 parts of natto, 13 parts of rhizoma polygonati extract and 6 parts of radix puerariae extract.
As an implementation mode of the embodiment of the application, the natto composition for conditioning intestines and stomach and reducing blood fat further comprises, in parts by weight: 1-3 parts of licorice extract.
Based on natto, rhizoma polygonati extract and radix puerariae extract, a small amount of licorice extract can be added in the application, so that the effect of harmonizing the medicine property is achieved.
In some embodiments, the licorice extract is a licorice aqueous extract, which may be prepared directly from commercial products or by existing aqueous extraction processes. Specifically, the preparation of the licorice water extract may comprise the following steps: adding the crushed liquorice into water with the weight of 8-15 times at the temperature of 95-105 ℃ for reflux extraction for 1-3 hours, collecting the extracting solution, concentrating under reduced pressure, and drying in vacuum to constant weight to obtain the liquorice water extract.
As an embodiment of the present application, the preparation method of the rhizoma polygonati extract includes the following steps:
adding the crushed rhizoma polygonati skin into ethanol with the volume fraction of 75-85% and the weight of 8-15 times of the crushed rhizoma polygonati skin at the temperature of 80-90 ℃ for reflux extraction for 1-3 hours, collecting an extracting solution, and concentrating under reduced pressure to obtain an extract;
loading the extract on a polyamide column, eluting with ethanol with the volume fraction of 15-20% which is 3-5 times of the column volume; eluting with ethanol with the volume fraction of 40-45% and the volume fraction of 6-10 times of the column volume, collecting ethanol eluate with the volume fraction of 40-45%, concentrating and drying to obtain the rhizoma polygonati extract.
The applicant finds that the sealwort extract obtained by adopting the mode of reflux extraction of ethanol with the volume fraction of 75-85 percent and collection of ethanol eluate with the volume fraction of 40-45 percent has better blood fat reducing effect when being further matched with natto and radix puerariae extract.
As an implementation mode of the embodiment of the application, the preparation method of the kudzuvine root extract comprises the following steps:
adding crushed radix Puerariae into water, performing reduced pressure distillation, stopping reduced pressure distillation when the volume of distillate is half of the volume of the initial water adding amount, collecting reduced pressure distillation extractive solution, concentrating and drying to obtain radix Puerariae extract;
wherein, the working parameters of the reduced pressure distillation extraction comprise: the temperature is 68-72 ℃, the pressure is 50-55 Kpa, and the extraction times are 1-3 times.
The applicant finds that the radix puerariae extract obtained by carrying out reduced pressure distillation under the condition of specific parameters has better blood fat reducing effect when being further matched with natto and rhizoma polygonati extract.
The components involved in the natto composition for regulating intestines and stomach and reducing blood fat provided by the embodiment of the application can be commercially available products if no special limitation or specific description exists; meanwhile, the steps and parameters involved in the preparation method can be carried out according to the existing technology unless specifically limited or specified.
In a second aspect, the application provides the application of the natto composition for regulating intestines and stomach and reducing blood fat in preparing a medicament for reducing blood fat and/or a health care product.
The natto composition provided by the embodiment of the application has obvious blood lipid reducing effect, can be applied to the preparation of blood lipid reducing medicines and/or health care products, and has practical application value.
In a third aspect, the present application provides a hypolipidemic drug, wherein the active ingredient of the hypolipidemic drug is the natto composition for conditioning intestines and stomach to reduce blood lipid according to any one of the first aspects.
In some specific embodiments, the hypolipidemic drug provided by the application can be added with pharmaceutical excipients adaptively according to actual dosage forms. The "pharmaceutical excipients" may include "pharmaceutical carriers" and "excipients" for pharmaceutical agents such as physiological saline, glucose, vitamin C, amino acids, etc., which can alter the manner and distribution of the drug into the body, control the release rate of the drug and deliver the drug to the targeted organ. Specifically, it refers to an addition to the main drug in the pharmaceutical preparation. Such as binders, fillers, disintegrants, lubricants in the tablet; wine, vinegar, medicinal juice and the like in the traditional Chinese medicine pill; a base portion in a semisolid formulation ointment, cream; preservative, antioxidant, correctant, aromatic, cosolvent, emulsifier, solubilizer, osmotic pressure regulator, colorant, etc. in liquid preparation.
In some embodiments, the dosage form of the hypolipidemic agent includes at least one of a granule, a capsule, a tablet, a granule, an oral liquid, and a powder.
The application will be further illustrated with reference to specific examples. It is to be understood that these examples are illustrative of the present application and are not intended to limit the scope of the present application. The experimental procedures, which are not specified in the following examples, are generally determined according to national standards. If the corresponding national standard does not exist, the method is carried out according to the general international standard, the conventional condition or the condition recommended by the manufacturer.
Example 1
The example provides a natto composition for regulating intestines and stomach and reducing blood fat, which comprises the following components in parts by weight (10 g for every 1 part by weight): 25 parts of natto, 8 parts of rhizoma polygonati extract and 5 parts of radix puerariae extract.
As shown in fig. 1, the preparation method of the natto composition for conditioning intestines and stomach and reducing blood fat comprises the following steps:
step (1), obtaining natto: soaking semen glycines in water for 24 hr; then pressurizing the soaked soybeans (under 2-10 atmospheric pressure) and steaming for 2 hours, cooling the steamed soybeans to room temperature, and uniformly spraying bacillus natto liquid; controlling the temperature of natto at 40 ℃ and the relative humidity at 80-90%, and fermenting for 14h; refrigerating fermented natto at 4 ℃, taking out, freeze-drying, crushing and sieving with a 50-60-mesh sieve to obtain the fermented natto;
step (2), obtaining a rhizoma polygonati extract: reflux-extracting pulverized rhizoma Polygonati in 80% ethanol 10 times of the total weight at 85deg.C for 2 hr, collecting extractive solution, and concentrating under reduced pressure to obtain extract; loading the extract on a polyamide column, eluting with ethanol with the volume fraction of 18% and 4 times of the column volume; eluting with 42% ethanol with 10 times of column volume, collecting 42% ethanol eluate, concentrating under reduced pressure, and vacuum drying to constant weight to obtain rhizoma Polygonati extract;
step (3), obtaining a kudzuvine root extract: adding the crushed kudzuvine root with a sieve of 30-50 meshes into water at the temperature of 70 ℃ and under the pressure of 53Kpa for first reduced pressure distillation extraction, and stopping reduced pressure distillation when the volume of distillate is half of the volume of the initial water adding amount; then adding half of the volume of the initial water adding amount, carrying out second reduced pressure distillation extraction under the same parameter condition, and stopping reduced pressure distillation when the volume of the distillate is half of the volume of the initial water adding amount; collecting and mixing the first vacuum distillation extractive solution and the second vacuum distillation extractive solution, concentrating under reduced pressure, and vacuum drying to constant weight to obtain radix Puerariae extract;
step (4): mixing the natto obtained in the step (1), the rhizoma polygonati extract obtained in the step (2) and the radix puerariae extract obtained in the step (3), grinding, and sieving with a 50-60-mesh sieve to obtain the natto composition for regulating intestines and stomach and reducing blood fat.
Example 2
This example provides a natto composition for regulating intestines and stomach and reducing blood lipid, which is different from example 1 only in that: the natto composition for conditioning intestines and stomach and reducing blood fat comprises the following components in parts by weight (10 g for every 1 part by weight): 45 parts of natto, 15 parts of rhizoma polygonati extract and 10 parts of radix puerariae extract; the rest steps and parameters are the same.
Example 3
This example provides a natto composition for regulating intestines and stomach and reducing blood lipid, which is different from example 1 only in that: the natto composition for conditioning intestines and stomach and reducing blood fat comprises the following components in parts by weight (10 g for every 1 part by weight): 37 parts of natto, 13 parts of rhizoma polygonati extract and 6 parts of radix puerariae extract; the rest steps and parameters are the same.
Example 4
The example provides a natto composition for regulating intestines and stomach and reducing blood fat, which comprises the following components in parts by weight (10 g for every 1 part by weight): 37 parts of natto, 13 parts of rhizoma polygonati extract, 6 parts of radix puerariae extract and 2 parts of liquorice extract.
The preparation method of the natto composition for regulating intestines and stomach and reducing blood fat comprises the following steps:
step (1), obtaining natto: soaking semen glycines in water for 24 hr; then pressurizing the soaked soybeans (under 2-10 atmospheric pressure) and steaming for 2 hours, cooling the steamed soybeans to room temperature, and uniformly spraying bacillus natto liquid; controlling the temperature of natto at 40 ℃ and the relative humidity at 80-90%, and fermenting for 14h; refrigerating fermented natto at 4 ℃, taking out, freeze-drying, crushing and sieving with a 50-60-mesh sieve to obtain the fermented natto;
step (2), obtaining a rhizoma polygonati extract: reflux-extracting pulverized rhizoma Polygonati skin with 10 times of 80% ethanol at 85deg.C for 2 hr, collecting extractive solution, and concentrating under reduced pressure to obtain extract; loading the extract on a polyamide column, eluting with ethanol with the volume fraction of 18% and 4 times of the column volume; eluting with 42% ethanol with 10 times of column volume, collecting 42% ethanol eluate, concentrating under reduced pressure, and vacuum drying to constant weight to obtain rhizoma Polygonati extract;
step (3), obtaining a kudzuvine root extract: adding crushed radix Puerariae into water at 70deg.C and 53Kpa pressure, performing first reduced pressure distillation extraction, and stopping reduced pressure distillation when the volume of distillate is half of the volume of the initial water addition amount; then adding half of the volume of the initial water adding amount, carrying out second reduced pressure distillation extraction under the same parameter condition, and stopping reduced pressure distillation when the volume of the distillate is half of the volume of the initial water adding amount; collecting and mixing the first vacuum distillation extractive solution and the second vacuum distillation extractive solution, concentrating under reduced pressure, and vacuum drying to constant weight to obtain radix Puerariae extract;
step (4), obtaining licorice extract: reflux extracting pulverized Glycyrrhrizae radix with 10 times of water at 100deg.C for 2 hr, collecting extractive solution, concentrating under reduced pressure, and vacuum drying to constant weight to obtain Glycyrrhrizae radix water extract;
step (5): mixing the natto obtained in the step (1), the rhizoma polygonati extract obtained in the step (2) and the radix puerariae extract obtained in the step (3) with the licorice extract obtained in the step (4), grinding, and sieving with a 50-60-mesh sieve to obtain the natto composition for regulating intestines and stomach and reducing blood fat.
Comparative example 1
This example provides a natto composition for regulating intestines and stomach and reducing blood lipid, which is different from example 1 only in that: regulating natto into red yeast rice; the rest steps and parameters are the same.
Comparative example 2
This example provides a natto composition for regulating intestines and stomach and reducing blood lipid, which is different from example 1 only in that: adjusting the rhizoma Polygonati extract to radix Codonopsis extract (prepared according to step (2) of the preparation method); the rest steps and parameters are the same.
Comparative example 3
This example provides a natto composition for regulating intestines and stomach and reducing blood lipid, which is different from example 1 only in that: adjusting the radix puerariae extract into a radix bupleuri extract (prepared according to the step (3) in the preparation method); the rest steps and parameters are the same.
Comparative example 4
This example provides a natto composition for regulating intestines and stomach and reducing blood lipid, which is different from example 1 only in that: the Polygonatum sibiricum extract is obtained in the preparation method step (2): reflux-extracting pulverized rhizoma Polygonati skin with 10 times of 80% ethanol at 85deg.C for 2 hr, collecting extractive solution, and concentrating under reduced pressure to obtain extract; loading the extract on a polyamide column, eluting with ethanol with the volume fraction of 18% and 4 times of the column volume; eluting with 65% ethanol with a volume fraction of 10 times of column volume, collecting 65% ethanol eluate, concentrating under reduced pressure, and vacuum drying to constant weight to obtain rhizoma Polygonati extract; the rest steps and parameters are the same.
Comparative example 5
This example provides a natto composition for regulating intestines and stomach and reducing blood lipid, which is different from example 1 only in that: in the preparation method, in the kudzu root extract obtained in the step (3): pulverizing radix Puerariae, sieving with 10 mesh sieve, adding 8 times of 60% ethanol, reflux extracting under heating for 4 times each for 1 hr, filtering with 100 mesh filter cloth, and mixing filtrates; placing the filtrate in HPD-100 macroporous adsorbent resin column chromatography, sequentially eluting with 3 times of column water and 5 times of column volume of 85% ethanol, collecting 85% ethanol eluate, recovering ethanol, concentrating under reduced pressure at 50deg.C and 0.08MPa until no alcohol is present, steaming in water bath at 80deg.C to obtain fluid extract, and drying in vacuum drying oven at 70deg.C and 0.08MPa to obtain radix Puerariae extract; the rest steps and parameters are the same.
Test example 1
The present example examined the hypolipidemic efficacy of the natto compositions for conditioning the intestines and stomach and reducing blood lipid provided in examples 1 to 4 and comparative examples 1 to 5.
The testing method comprises the following steps: the method comprises the steps of equally dividing 110 cleaning-grade Wistar rats with male and female halves into 11 groups of 10 rats each; the test groups are normal groups, high-fat control groups and experimental groups 1 to 9 respectively. The normal group was fed with only ordinary basal feed every day, the high fat control group was fed with only high fat feed every day (high fat feed: 10.0% sucrose, egg yolk powder 10%, 15% lard, 1.2% cholesterol, 0.2% sodium cholate and 63.6% ordinary feed), the experimental groups 1 to 9 were fed with high fat feed each day with the natto composition for regulating gastrointestinal hypolipidemia provided in examples 1 to 4 and comparative examples 1 to 5, respectively, and SD rats of each group were free to drink water. SD rats were sacrificed after 30 days of continuous feeding, and the total serum cholesterol TC and triglyceride TG content of each group of SD rats were determined after intraperitoneal venous blood sampling. Wherein, the natto composition in experiment group 1 to 9 is added in an amount of 5mg/kg to the daily fed high fat feed, experiment group 1 corresponds to the natto composition for regulating gastrointestinal blood lipid obtained in example 1, experiment group 2 corresponds to the natto composition for regulating gastrointestinal blood lipid obtained in example 2, experiment group 3 corresponds to the natto composition for regulating gastrointestinal blood lipid obtained in example 3, experiment group 4 corresponds to the natto composition for regulating gastrointestinal blood lipid obtained in example 4, experiment group 5 corresponds to the natto composition for regulating gastrointestinal blood lipid obtained in comparative example 1, experiment group 6 corresponds to the natto composition for regulating gastrointestinal blood lipid obtained in comparative example 2, experiment group 7 corresponds to the natto composition for regulating gastrointestinal blood lipid obtained in comparative example 3, experiment group 8 corresponds to the natto composition for regulating gastrointestinal blood lipid obtained in comparative example 4, and experiment group 9 corresponds to the natto composition for regulating gastrointestinal blood lipid obtained in comparative example 5.
The test results are shown in Table 1.
TABLE 1
| Group of | TC content (mmol/L) | TG content (mmol/L) |
| Normal group | 1.61±0.24 | 1.12±0.30 |
| High fat control group | 5.25±0.41 | 2.24±0.42 |
| Experiment group 1 | 2.31±0.28 | 1.82±0.39 |
| Experiment group 2 | 2.24±0.43 | 1.76±0.26 |
| Experiment group 3 | 1.71±0.40 | 1.23±0.33 |
| Experiment group 4 | 1.68±0.35 | 1.20±0.29 |
| Experiment group 5 | 4.91±0.45 | 2.12±0.27 |
| Experiment group 6 | 4.73±0.34 | 2.09±0.19 |
| Experiment group 7 | 5.09±0.36 | 2.01±0.22 |
| Experiment group 8 | 4.09±0.23 | 1.89±0.18 |
| Experiment group 9 | 4.35±0.44 | 1.91±0.38 |
As can be seen from Table 1, the natto compositions for regulating intestines and stomach and reducing blood lipid provided by examples 1 to 4 of the present application are superior to the natto compositions for regulating intestines and stomach and reducing blood lipid provided by comparative examples 1 to 5 in terms of blood lipid reduction. Therefore, the natto composition provided by the embodiment of the application has excellent blood lipid reducing effect, can provide more drug choices for patients with hyperlipidemia, and widens the application range of rhizoma polygonati, radix puerariae and the like.
Test example 2
50 volunteers suffering from dyspepsia and constipation are selected, the natto composition for regulating intestines and stomach and reducing blood fat provided in the embodiment 4 is taken, a proper amount of pharmaceutical auxiliary materials are added to prepare tablets, the daily dosage is 5g, after the oral administration is carried out for 2 weeks, the symptoms of dyspepsia and constipation of 40 volunteers disappear, the symptoms of dyspepsia and constipation of 5 volunteers are improved, the symptoms of dyspepsia and constipation of 5 volunteers are not obviously improved, the total effective rate is 90%, and the tablet has excellent regulating effect on intestines and stomach.
Various embodiments of the application may exist in a range of forms; it should be understood that the description in a range format is merely for convenience and brevity and should not be construed as a rigid limitation on the scope of the application; it is therefore to be understood that the range description has specifically disclosed all possible sub-ranges and individual values within that range. For example, it should be considered that a description of a range from 1 to 6 has specifically disclosed sub-ranges, such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6, etc., as well as single numbers within the range, such as 1, 2, 3, 4, 5, and 6, wherever applicable. In addition, whenever a numerical range is referred to herein, it is meant to include any reference number (fractional or integer) within the indicated range.
In the present application, unless otherwise specified, terms such as "upper" and "lower" are used specifically to refer to the orientation of the drawing in the figures. In addition, in the description of the present specification, the terms "include", "comprising" and the like mean "including but not limited to". Relational terms such as "first" and "second", and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Herein, "and/or" describing an association relationship of an association object means that there may be three relationships, for example, a and/or B, may mean: a alone, a and B together, and B alone. Wherein A, B may be singular or plural. Herein, "at least one" means one or more, and "a plurality" means two or more. "at least one", "at least one" or the like refer to any combination of these items, including any combination of single item(s) or plural items(s). For example, "at least one (individual) of a, b, or c," or "at least one (individual) of a, b, and c," may each represent: a, b, c, a-b (i.e., a and b), a-c, b-c, or a-b-c, wherein a, b, c may be single or multiple, respectively.
The foregoing is only a specific embodiment of the application to enable those skilled in the art to understand or practice the application. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the application. Thus, the present application is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (10)
1. The natto composition for regulating intestines and stomach and reducing blood fat is characterized by comprising natto, rhizoma polygonati extract and radix puerariae extract.
2. The natto composition for regulating gastrointestinal blood lipid according to claim 1, wherein the natto composition for regulating gastrointestinal blood lipid comprises the following components in parts by weight:
25-45 parts of natto, 8-15 parts of rhizoma polygonati extract and 5-10 parts of radix puerariae extract.
3. The gastrointestinal hypolipidemic natto composition of claim 2, further comprising, in parts by weight: 1-3 parts of licorice extract.
4. The gastrointestinal hypolipidemic natto composition of claim 2, wherein the gastrointestinal hypolipidemic natto composition comprises the following components in parts by weight:
37 parts of natto, 13 parts of rhizoma polygonati extract and 6 parts of radix puerariae extract.
5. The natto composition for regulating gastrointestinal blood lipid lowering according to any one of claims 1 to 4, wherein the preparation method of the rhizoma polygonati extract comprises the following steps:
adding the crushed rhizoma polygonati skin into ethanol with the volume fraction of 75-85% and the weight of 8-15 times of the crushed rhizoma polygonati skin at the temperature of 80-90 ℃ for reflux extraction for 1-3 hours, collecting an extracting solution, and concentrating under reduced pressure to obtain an extract;
loading the extract on a polyamide column, eluting with ethanol with the volume fraction of 15-20% which is 3-5 times of the column volume; eluting with ethanol with the volume fraction of 40-45% and the volume fraction of 6-10 times of the column volume, collecting ethanol eluate with the volume fraction of 40-45%, concentrating and drying to obtain the rhizoma polygonati extract.
6. The natto composition for regulating gastrointestinal blood lipid lowering according to any one of claims 1 to 4, wherein the preparation method of the kudzuvine root extract comprises the following steps:
adding crushed radix Puerariae into water, performing reduced pressure distillation, stopping reduced pressure distillation when the volume of distillate is half of the volume of the initial water adding amount, collecting reduced pressure distillation extractive solution, concentrating and drying to obtain radix Puerariae extract;
wherein, the working parameters of the reduced pressure distillation extraction comprise: the temperature is 68-72 ℃, the pressure is 50-55 Kpa, and the extraction times are 1-3 times.
7. The gastrointestinal hypolipidemic natto composition of any one of claims 1 to 4, wherein the licorice extract is an aqueous licorice extract.
8. Use of a natto composition for regulating gastrointestinal blood lipid lowering according to any one of claims 1 to 7 in the preparation of a medicament for lowering blood lipid and/or a health product.
9. A hypolipidemic drug, characterized in that the active ingredient of the hypolipidemic drug is the natto composition for conditioning intestines and stomach and reducing blood lipid as claimed in any one of claims 1 to 7.
10. The hypolipidemic drug of claim 9, wherein the hypolipidemic drug is in a dosage form comprising at least one of a granule, capsule, tablet, granule, oral liquid, and powder.
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| CN117652544A (en) * | 2023-12-26 | 2024-03-08 | 久睦本(海南)科技股份有限公司 | A kind of hypolipidemic functional food containing natto powder extract and its preparation method and use |
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| CN103223033A (en) * | 2013-05-22 | 2013-07-31 | 武汉真福医药科技发展有限公司 | Novel natto health care composition and its preparation method and application |
| CN105641418A (en) * | 2016-02-06 | 2016-06-08 | 刘会武 | Formula of natto and preparation method |
| CN114533817A (en) * | 2020-11-24 | 2022-05-27 | 内蒙古伊利实业集团股份有限公司 | Composition for improving blood sugar and/or blood fat and application thereof |
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| CN103223033A (en) * | 2013-05-22 | 2013-07-31 | 武汉真福医药科技发展有限公司 | Novel natto health care composition and its preparation method and application |
| CN105641418A (en) * | 2016-02-06 | 2016-06-08 | 刘会武 | Formula of natto and preparation method |
| CN114533817A (en) * | 2020-11-24 | 2022-05-27 | 内蒙古伊利实业集团股份有限公司 | Composition for improving blood sugar and/or blood fat and application thereof |
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| CN117652544A (en) * | 2023-12-26 | 2024-03-08 | 久睦本(海南)科技股份有限公司 | A kind of hypolipidemic functional food containing natto powder extract and its preparation method and use |
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