CN116751662A - Separator with cytoprotection function - Google Patents
Separator with cytoprotection function Download PDFInfo
- Publication number
- CN116751662A CN116751662A CN202311037821.8A CN202311037821A CN116751662A CN 116751662 A CN116751662 A CN 116751662A CN 202311037821 A CN202311037821 A CN 202311037821A CN 116751662 A CN116751662 A CN 116751662A
- Authority
- CN
- China
- Prior art keywords
- box
- sealing
- wall
- plate
- separator
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000007789 sealing Methods 0.000 claims abstract description 124
- 239000007788 liquid Substances 0.000 claims abstract description 57
- 238000000926 separation method Methods 0.000 claims abstract description 43
- 238000003825 pressing Methods 0.000 claims description 26
- 239000012530 fluid Substances 0.000 claims description 12
- 230000001120 cytoprotective effect Effects 0.000 claims description 8
- 238000000605 extraction Methods 0.000 abstract description 17
- 230000006378 damage Effects 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 230000000694 effects Effects 0.000 description 11
- 230000009471 action Effects 0.000 description 8
- 239000008280 blood Substances 0.000 description 7
- 210000004369 blood Anatomy 0.000 description 7
- 238000005119 centrifugation Methods 0.000 description 3
- 238000006073 displacement reaction Methods 0.000 description 3
- 238000004891 communication Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/10—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by centrifugation ; Cyclones
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Centrifugal Separators (AREA)
Abstract
The invention relates to the technical field of cell separation equipment, in particular to a separator with a cell protection function, which comprises a separation box, a separation motor, a sealing box, a liquid outlet box, a collection box and a sealing cavity, wherein the separation box is internally and rotatably connected with a separation cylinder, the bottom of the separation cylinder is connected with the output end of the separation motor, the outer wall of the bottom of the separation cylinder is provided with a liquid outlet hole, and the interior of the liquid outlet hole is slidably connected with a sealing block; the beneficial effects of the invention are as follows: through the setting of separating drum and collecting box, protection cell when the separation, can the automatic collection after the separation, avoid the cell that artifical contact leads to receive destruction, reduce artificial contact, this device is when carrying out automatic collection to every layer of cell simultaneously, can realize automatic extraction different layers of cells, realize collecting in different classification incasement, avoid appearing the mixed phenomenon of cell after the separation, prevent that every layer of extraction that artifical extraction leads to from not thoroughly, the phenomenon of intermixing.
Description
Technical Field
The invention relates to the technical field of cell separation equipment, in particular to a separator with a cell protection function.
Background
In order to detect the condition of cells, it is necessary to extract blood or the like for detection in order to understand some physical health information, and in order to understand each item of information of cells more precisely, it is generally necessary to separate cells.
Chinese patent (CN 215901976U) discloses a cell centrifugal separation device for gene detection, which comprises a workbench and is characterized in that, the upside of workstation is located both ends position department symmetry and is fixed with two side riser, two the upper end of side riser all is provided with the mounting panel, two one side of mounting panel all rotates and is connected with the pivot, two be fixed with the support between the pivot, one of them the upset motor is installed to one side of mounting panel, the drive end of upset motor is connected with one of them pivot's one end, the downside of support is located middle section position department and installs centrifugal motor.
Above-mentioned patent is through pressing from both sides tight fixed to centrifugal container through pressing from both sides tight actuating mechanism, centrifugal container stability when high-speed rotation has improved centrifugal quality, simultaneously through container washing blow-dry mechanism, can wash and blow-dry centrifugal container automatically, but after the separation finishes, when extracting different layer cells, need the manual work to extract, greatly increased the number of times of manual work contact, lead to the data to appear the error easily, the manual work is extracted and is unable accurate to extract every layer of cell sap, the deviation that appears that leads to the extraction can not realize automatic extraction, greatly reduced separating efficiency.
Therefore, a separator having a cytoprotective function is required to solve the above-mentioned problems.
Disclosure of Invention
In order to solve the above problems, namely, to solve the problems of automatically extracting and storing each layer of different cell fluids, the invention provides a separator with a cytoprotection function.
The utility model provides a separator with cytoprotection function, includes separator box, separation motor, seal box, goes out liquid box, collecting box and seal chamber, the inside rotation of separator box is connected with the separating drum, the bottom of separating drum is connected with the output of separation motor, the liquid outlet has been seted up on the outer wall of separating drum bottom, the inside sliding connection who goes out the liquid outlet has the sealing block, the liquid outlet has one end sealing connection and the fluid conduction of seal box, the inside sliding connection of seal box has the centrifugal piece, the other end and the one end sealing connection and the fluid conduction of play liquid box of seal box, the inside sliding connection of play liquid box has the seal shell, the seal shell with the one end of seal box supports the top, the other end and the one end sealing connection and the fluid conduction of collecting box of play liquid box, collecting box fixed mounting is in on the lateral wall of separator box, the internally mounted of collecting box has the classifying box, the quantity of classifying box is two or more, the top of separator box articulates there is sealed lid, sealed lid and seal chamber sealing connection, the inside sealing chamber is located the inside wall of separating box.
Above-mentioned separator with cytoprotection function, friction line has been seted up on the lateral wall of sealing block, sealing block's top and sealing spring's one end fixed connection, sealing spring's the other end with the inner wall fixed connection of separating drum, sealing block's outer wall and the outer wall fixed connection of pressing the pole, pressing the bottom sliding connection of pressing the pole is in sealing box's inside.
Above-mentioned separator with cytoprotection function, the inside fixed mounting of seal box has the baffle, the seal groove has been seted up to the inner wall of baffle, the one end of centrifugal piece and reset spring's one end fixed connection, reset spring's the other end with seal box inner wall fixed connection, friction line has been seted up on the lateral wall of centrifugal piece, centrifugal piece is kept away from fixed mounting has the drive pinion rack on the outer wall of reset spring one end.
Above-mentioned separator with cytoprotection function, sliding connection has the slip box on the exit end of seal box, the inside rotation of the lateral wall of slip box is connected with drive gear, drive gear's outer wall and drive pinion rack meshing are connected, fixed tooth plate is fixed mounting on the outer wall at slip box top, fixed tooth plate and drive gear meshing are connected.
Above-mentioned separator with cytoprotection function, sliding connection has the slide on the lateral wall of play liquid box, the slide is located go out the outside one end of liquid box with the outer wall of slip box supports the top, the other end of slide sets up to the inclined plane, and supports with the top of briquetting down, the briquetting diminishes from top to bottom, the bottom of pressing the pole is located the lateral wall of slide one end, install the speed limit board on the outer wall of liquid box, the speed limit board with the outer wall of seal box supports the top.
Above-mentioned separator with cytoprotection function, sliding connection has the slide on the lateral wall of play liquid box, the slide is located go out the outside one end of liquid box with the outer wall of slip box supports the top, the other end of slide sets up to the inclined plane, and supports with the top of briquetting down, the briquetting diminishes from top to bottom, the bottom of pressing the pole is located the lateral wall of slide one end, install the speed limit board on the outer wall of liquid box, the speed limit board with the outer wall of seal box supports the top.
Above-mentioned separator with cytoprotection function, sliding connection has the gag lever post on the inner wall of classifying box, install flexible closing plate on the outer wall of gag lever post, the gag lever post is located the bottom fixed mounting of the inside one end of classifying box has the cardboard, the inside sliding connection of classifying box has the kickboard, fixed mounting has the card case on the outer wall of kickboard one end, the inside and the one end fixed connection of drive line of card case, the other end and the outer wall fixed connection of flexible axis of rotation of drive line, flexible axis of rotation rotates to be connected the top of kickboard, the top of flexible axis of rotation rotates to be connected the inside of self-interacting piece.
Above-mentioned separator with cytoprotection function, fixed mounting is supported the kicking block on the outer wall at flexible axis of rotation top, support the kicking block and support the one end of ejector pin and support the top, support the other end of ejector pin with the one end fixed connection of filling board, filling board sliding connection is in the inside of self-interacting piece, the outer wall of supporting the ejector pin and the one end fixed connection of connecting wire, the other end of connecting wire with support the outer wall fixed connection of kicking block, be provided with the scale on the outer wall of classifying box.
Above-mentioned separator with cytoprotection function, the inside rotation of separator case is connected with the drive shaft, the one end of drive shaft with the output drive connection of separation motor, sliding connection has the slide shaft on the outer wall of the drive shaft other end, slide shaft sliding connection is in on the inner wall of separator case, install the rotation gear on the outer wall of slide shaft, rotation gear and driven gear meshing are connected, driven gear installs on the outer wall of drive lead screw, drive lead screw rotates to be connected in the inside of sealed chamber, threaded connection has the flitch on the outer wall of drive lead screw, the screw thread at the both ends of drive lead screw is opposite.
Above-mentioned separator with cytoprotection function, the bottom fixed mounting of sealed lid has the closing plate, the closing plate joint is in the inside of sealed chamber.
The beneficial effects of the invention are as follows:
1. according to the invention, through the arrangement of the separating cylinder and the collecting box, cells can be automatically separated, a large amount of cells can be treated at one time, separation of each layer of cells is protected, layering after separation is realized, meanwhile, the device can be automatically collected after separation, damage to the cells caused by manual contact is avoided, manual contact is reduced, meanwhile, when the device automatically collects each layer of cells, automatic extraction of different layers of cells is realized, collection in different classifying boxes is realized, the phenomenon of mixing of the separated cells is avoided, incomplete extraction of each layer caused by manual extraction is prevented, and the phenomenon of mixing each other is realized.
2. When the centrifugal separation is carried out, the automatic sealing of the liquid outlet hole can be realized when the separation motor is started, the phenomenon that blood leaks when the separation occurs is avoided, meanwhile, the device can automatically open the outlet after the separation is stopped, the cell layer is extracted, the liquid outlet of the device can be closed along with the rotation of the separation cylinder, the automation can be realized when the separation motor is stopped, meanwhile, the driving toothed plate of the device plays a role in guiding, the phenomenon that the centrifugal block deflects under the action of the centrifugation is avoided, and the centrifugal block can be ensured to accurately seal the sealing groove.
3. The centrifugal block is sealed or sealed slowly, the sliding box can be reset, the separated blood can be precipitated through the slow reset of the centrifugal block, sufficient precipitation time is ensured, the phenomenon that the separated blood is not precipitated and is immediately extracted is avoided, meanwhile, the device can play a role in decelerating a separating cylinder which is still rotated under the inertia effect after stopping through the arrangement of the speed limiting plate, and can realize stopping under the action of the speed limiting plate when the speed is slowed down, and the sliding box is automatically clamped, so that the sliding box plays a guiding role, the phenomenon that the outlets are not contacted is avoided, the continuous high-speed rotation is avoided, and the phenomenon that the separating cylinder cannot be clamped after rotation is avoided.
4. According to the invention, through changing the arrangement of the flow plates, cell liquid can flow on two sides, after extraction is finished, the first liquid leakage hole can be automatically sealed, the backward flow can be performed only after the first liquid leakage hole is sealed, and then the cell liquid flows into the second classification box, so that automatic classification collection is realized, and the automatic collection can be realized only by adjusting the height of the limiting rod at first, so that the phenomenon of excessive collection is avoided.
5. The diameter of the rotating gear is far larger than that of the driven gear, the pinion is driven to rotate by the large gear, the phenomenon that the displacement distance of the pushing plate is too large due to the fact that the driving screw rotates too fast is avoided, meanwhile, the device can automatically seal when the device is separated through the arrangement of the sealing plate and the pushing plate, the leakage phenomenon is avoided, and meanwhile, the longer the device is operated, the more tightly the sealing is, and the sealing effect of the device is guaranteed.
Drawings
FIG. 1 is a schematic diagram of a front perspective view of the present invention;
FIG. 2 is a schematic cross-sectional view of a separator tank according to the present invention;
FIG. 3 is a schematic cross-sectional view of a separator cartridge of the present invention;
FIG. 4 is a schematic cross-sectional view of a seal cartridge of the present invention;
FIG. 5 is an enlarged schematic view of the structure of FIG. 4A according to the present invention;
FIG. 6 is a schematic cross-sectional view of a liquid outlet box according to the present invention;
FIG. 7 is a schematic cross-sectional view of the collection box of the present invention;
FIG. 8 is a schematic cross-sectional structural view of the classification box of the present invention;
FIG. 9 is a schematic view of the structure of the inside of the card case of the present invention;
FIG. 10 is a schematic top view of a self-adjusting block of the present invention;
FIG. 11 is a schematic view of the structure of the inside of the side wall of the separator of the present invention;
fig. 12 is a schematic structural view of the sealing cap of the present invention.
In the figure:
1. a separation box; 2. a separation cylinder; 3. separating the motor; 4. a sealing block; 5. a seal box; 6. a centrifugal block; 7. a liquid outlet box; 8. a sealed housing; 9. a collection box; 10. a classification box; 11. sealing cover; 12. sealing the cavity; 13. a seal spring; 14. pressing a pressing rod; 15. a baffle; 16. a return spring; 17. driving the toothed plate; 18. a slide box; 19. a drive gear; 20. fixing the toothed plate; 21. a slide plate; 22. pressing the block; 23. a speed limiting plate; 24. a partition plate; 25. a flow changing plate; 26. a self-adjusting block; 27. a limit rod; 28. a clamping plate; 29. a floating plate; 30. a card box; 31. a driving line; 32. a telescopic rotating shaft; 33. a pushing block; 34. a push rod; 35. a filler plate; 36. a connecting wire; 37. a scale; 38. a drive shaft; 39. a slide shaft; 40. rotating the gear; 41. a driven gear; 42. driving a screw rod; 43. a pushing plate; 44. and (5) sealing the plate.
Detailed Description
Preferred embodiments of the present invention are described below with reference to the accompanying drawings. It should be understood by those skilled in the art that these embodiments are merely for explaining the technical principles of the present invention, and are not intended to limit the scope of the present invention.
1-4, 6 and 11, the embodiment of the invention discloses a separator with a cytoprotective function, which comprises a separating box 1, a separating motor 3, a sealing box 5, a liquid outlet box 7, a collecting box 9 and a sealing cavity 12, wherein the separating box 1 is internally and rotatably connected with a separating cylinder 2, the bottom of the separating cylinder 2 is connected with the output end of the separating motor 3, a liquid outlet hole is formed in the outer wall of the bottom of the separating cylinder 2, the sealing block 4 is slidingly connected in the liquid outlet hole, the liquid outlet hole is hermetically connected with one end of the sealing box 5 and is in fluid conduction, the centrifugal block 6 is slidingly connected in the sealing box 5, the other end of the sealing box 5 is hermetically connected with one end of the liquid outlet box 7 and is in fluid conduction, the sealing shell 8 is propped against one end of the sealing box 5, the other end of the liquid outlet box 7 is hermetically connected with one end of the collecting box 9 and is in fluid conduction, the collecting box 9 is fixedly arranged on the side wall of the separating box 1, the classifying box 10 is internally arranged in the collecting box 9, the quantity of the classifying box 10 is two or more than two sealing boxes 1 are hermetically connected with one end of the sealing box 11, and the sealing box 11 is hermetically connected with the sealing box 11 on the sealing cavity 11 in the sealing box 11, and the sealing box 11 is hermetically connected with the sealing cavity 11 on the top of the sealing box 11 is shown in the sealing box 11 is in the sealing box 11 and is shown in the sealing 11 is in the sealing 12 is in the sealing box 12; this device can separate the cell through the setting of separating drum 2 and collection box 9 voluntarily, can once only handle in a large number simultaneously, the separation of protection every layer of cell, realize the layering after the separation, can the automatic collection after this device separates simultaneously, avoid the cell that manual contact leads to receive destruction, reduce artificial contact, this device is when carrying out automatic collection to every layer of cell simultaneously, can realize the automatic extraction different layer of cells, realize collecting in different classification boxes 10, avoid appearing the mixed phenomenon of cell after the separation, prevent that every layer of extraction that manual extraction leads to is incomplete, the phenomenon of intermixing, this device is after collecting, can be according to the volume of adjusting classification box 10 extraction, realize automatic extraction different cell layers, save respectively, the separation effect is improved, ensure experimental data's accuracy.
As shown in fig. 2-3, friction lines are formed on the side wall of the sealing block 4, the top of the sealing block 4 is fixedly connected with one end of the sealing spring 13, the other end of the sealing spring 13 is fixedly connected with the inner wall of the separating cylinder 2, the outer wall of the sealing block 4 is fixedly connected with the outer wall of the pressing rod 14, and the bottom of the pressing rod 14 is slidably connected in the sealing box 5; when separating, firstly, the pressing rod 14 is pressed, so that the pressing rod 14 drives the sealing block 4 to descend, at the moment, the sealing block 4 seals the liquid outlet hole, then blood is poured into the separating cylinder 2, the pressing rod 14 is loosened, at the moment, under the action of friction lines, the pressing rod sealing block 4 is slowly reset, the liquid outlet hole is in a sealed state, and at the moment, the separating motor 3 is started to separate.
As shown in fig. 4, a baffle 15 is fixedly installed in the seal box 5, a seal groove is formed in the inner wall of the baffle 15, one end of the centrifugal block 6 is fixedly connected with one end of the return spring 16, the other end of the return spring 16 is fixedly connected with the inner wall of the seal box 5, friction lines are formed in the side wall of the centrifugal block 6, and a driving toothed plate 17 is fixedly installed on the outer wall of one end, far away from the return spring 16, of the centrifugal block 6.
As shown in fig. 4-5, a sliding box 18 is slidingly connected to the outlet end of the sealing box 5, a driving gear 19 is rotatably connected to the inside of the side wall of the sliding box 18, the outer wall of the driving gear 19 is meshed with a driving toothed plate 17, a fixed toothed plate 20 is fixedly mounted on the outer wall of the top of the sliding box 18, and the fixed toothed plate 20 is meshed with the driving gear 19; in the initial state of the device, the centrifugal block 6 is not contacted with the sealing groove, the driving toothed plate 17 is not connected with the driving gear 19, the centrifugal block 6 can slide under the centrifugal action when the device performs centrifugal separation, the sealing groove on the centrifugal block 6 and the baffle 15 is propped against at the moment, the sealing of the sealing groove is realized, at the moment, the continuous displacement of the centrifugal block 6 can lead the driving toothed plate 17 to be meshed and connected with the driving gear 19, at the moment, the driving gear 19 drives the fixed toothed plate 20 to displace, and the sliding box 18 slides back to the inside of the sealing box 5; when this device carries out centrifugal separation, can realize the self-sealing to the liquid hole when separating motor 3 starts, when avoiding appearing separating, the phenomenon that blood appears revealing, this device can open the export voluntarily after the separation stops simultaneously, realize extracting the cell layer, realize that the liquid outlet of this device follows the rotation of separating drum 2 and is closed, can open voluntarily when stopping, realize automaticly, the drive pinion rack 17 of this device plays the effect of direction simultaneously, avoid centrifugal piece 6 to appear the phenomenon of skew under the effect of centrifugation, guarantee that centrifugal piece 6 can be right seals the seal groove.
As shown in fig. 6, a sliding plate 21 is slidably connected to the side wall of the liquid outlet box 7, one end of the sliding plate 21 positioned outside the liquid outlet box 7 abuts against the outer wall of the sliding box 18, the other end of the sliding plate 21 is provided with an inclined plane and abuts against the top of a lower pressing block 22, the width of the lower pressing block 22 is reduced from top to bottom, the bottom of the pressing rod 14 is positioned on the side wall of one end of the sliding plate 21, a speed limiting plate 23 is mounted on the outer wall of the liquid outlet box 7, and the speed limiting plate 23 abuts against the outer wall of the sealing box 5; in the initial state of the device, the sealing shell 8 is clamped in the sliding box 18, when the pressing rod 14 is pressed, the pressing rod 14 is simultaneously driven to abut against the side wall at one end of the sliding plate 21, so that the sliding plate 21 pushes the sliding box 18 to reset, at the moment, the sealing shell 8 is disconnected with the sliding box 18, the sealing shell 8 slides into the liquid outlet box 7, the centrifugal block 6 can realize sealing of the sealing groove by a short distance, under the action of long-time centrifugation, the centrifugal block 6 can continuously displace, under the action of friction lines and the like, the centrifugal block 6 slowly displaces, so that the driving toothed plate 17 drives the sliding box 18 to displace inwards, after stopping, the centrifugal block 6 resets under the action of the reset spring 16, and meanwhile resets slowly, at the moment, the driving gear 19 reversely rotates, the sliding box 18 abuts against the sliding plate 21, so that the sealing shell 8 slides into the sliding box 18, the sealing effect is realized, and at the same time, the speed limiting plate 23 abuts against the outer wall of the sealing box 5; the centrifugal block 6 of this device is sealed or sealed all is slower, can satisfy the reset of slip box 18, when the slow reset through centrifugal block 6, can make the blood after separating deposit, guarantee sufficient sedimentation time, avoid appearing not depositing and accomplish and draw immediately, this device can play the effect of slowing down to still rotating separating tube 2 under inertial action after stopping through the setting of speed limiting plate 23 simultaneously, when the speed slows down, can realize stopping under the effect of speed limiting plate 23 to make the automatic joint of slip box 18, play the effect of direction, avoid the phenomenon of contactless between the export, avoid continuous high-speed rotation, and the phenomenon of unable joint after rotatory.
As shown in fig. 7, a baffle plate 24 is fixedly installed in the collecting box 9, liquid leakage holes are formed in the inner wall of the baffle plate 24, the liquid leakage holes are in sealing connection with the top of the classifying box 10 and are in fluid communication, a flow changing plate 25 is fixedly installed at the top of the baffle plate 24, self-adjusting blocks 26 are installed on the inner wall of the liquid leakage holes, and the number of the self-adjusting blocks 26 is two or more; when extracting the cell sap, the cell sap flows into the inside of classifying box 10 by first weeping hole at first, and when the extraction, the effect that changes the class is played to the direction to the fine cell sap of extraction to change the class board 25 for the cell sap has the both sides of changing class board 25 to flow, and the baffle 24 of this device is provided with certain inclination, is convenient for extract.
As shown in fig. 8-9, a limiting rod 27 is slidably connected to the inner wall of the classifying box 10, a telescopic sealing plate is mounted on the outer wall of the limiting rod 27, a clamping plate 28 is fixedly mounted at the bottom of one end of the inside of the classifying box 10, a floating plate 29 is slidably connected to the inside of the classifying box 10, a clamping box 30 is fixedly mounted on the outer wall of one end of the floating plate 29, the inside of the clamping box 30 is fixedly connected with one end of a driving wire 31, the other end of the driving wire 31 is fixedly connected with the outer wall of a telescopic rotating shaft 32, the telescopic rotating shaft 32 is rotatably connected to the top of the floating plate 29, and the top of the telescopic rotating shaft 32 is rotatably connected to the inside of the self-adjusting block 26.
As shown in fig. 7 and 10, a propping block 33 is fixedly installed on the outer wall of the top of the telescopic rotating shaft 32, the propping block 33 is propped against one end of a propping rod 34, the other end of the propping rod 34 is fixedly connected with one end of a filling plate 35, the filling plate 35 is slidably connected in the self-adjusting block 26, the outer wall of the propping rod 34 is fixedly connected with one end of a connecting wire 36, the other end of the connecting wire 36 is fixedly connected with the outer wall of the propping block 33, and a scale 37 is arranged on the outer wall of the classifying box 10; firstly, the height of the limiting rod 27 is adjusted, comparison is carried out through a scale 37, after the adjustment is finished, cell liquid enters the first classification box 10, at the moment, the floating plate 29 rises, after the floating plate rises to the position of the limiting rod 27 after adjustment, a layer of cells are extracted and stored, the clamping box 30 on the floating plate 29 is propped against the clamping plate 28, at the moment, the clamping plate 28 is propped against the driving wire 31, the driving wire 31 is stretched, the driving wire 31 drives the telescopic rotating shaft 32 to rotate, at the moment, the propping block 33 at the top of the telescopic rotating shaft 32 is propped against the propping rod 34, the propping rod 34 drives the filling plate 35 to displace, the filling plate 35 seals a liquid leakage hole, at the moment, the cell liquid continuously extracted flows into the second classification box, and is sequentially extracted in a reciprocating manner; this device can make the cell sap flow both sides again through changing the setting of flow board 25, can seal first weeping hole voluntarily after the extraction finishes, only just can carry out the backward flow after first weeping hole seals, then flows into the inside of second classification case 10, realizes automatic classification collection, only need through the high realization automatic collection that can realize of adjusting gag lever post 27 at first, avoids appearing collecting too much phenomenon.
As shown in fig. 11, the inside of the separation box 1 is rotatably connected with a driving shaft 38, one end of the driving shaft 38 is in driving connection with the output end of the separation motor 3, a sliding shaft 39 is slidably connected on the outer wall of the other end of the driving shaft 38, the sliding shaft 39 is slidably connected on the inner wall of the separation box 1, a rotating gear 40 is mounted on the outer wall of the sliding shaft 39, the rotating gear 40 is in meshing connection with a driven gear 41, the driven gear 41 is mounted on the outer wall of a driving screw 42, the driving screw 42 is rotatably connected inside the sealing cavity 12, a pushing plate 43 is in threaded connection with the outer wall of the driving screw 42, and threads at two ends of the driving screw 42 are opposite.
As shown in fig. 12, a sealing plate 44 is fixedly installed at the bottom of the sealing cover 11, and the sealing plate 44 is clamped in the sealing cavity 12; the inner wall of the sealing plate 44 of the device is provided with a pushing groove, meanwhile, the sealing plate 44 is made of sealing rubber, when the device is used, the sealing plate 44 at the bottom of the sealing cover 11 is clamped in the sealing cavity 12, at the moment, the side wall of the pushing plate 43 is propped against the pushing groove on the inner wall of the sealing plate 44, after the separation motor 3 is started, the driving shaft 38 drives the rotating gear 40 to rotate, the driven gear 41 drives the driving screw 42 to rotate, the pushing plate 43 displaces in the pushing groove, the outer wall of the sealing plate 44 tightly props against the outer wall of the sealing cavity 12, and the sealing of the sealing cavity 12 and the separation box 1 is realized; the diameter of the rotating gear 40 of the device is far larger than that of the driven gear 41, the large gear drives the pinion to rotate, the phenomenon that the displacement distance of the pushing plate 43 is too large due to the fact that the driving screw 42 rotates too fast is avoided, meanwhile, the device can automatically seal when being separated through the sealing plate 44 and the pushing plate 43, the phenomenon of leakage is avoided, meanwhile, the longer the device is, the more tightly sealed, and the sealing effect of the device is guaranteed.
It should be noted that, in the description of the present invention, terms such as "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like, which indicate directions or positional relationships, are based on the directions or positional relationships shown in the drawings, are merely for convenience of description, and do not indicate or imply that the apparatus or elements must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
Furthermore, it should be noted that, in the description of the present invention, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention can be understood by those skilled in the art according to the specific circumstances.
The terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, article, or apparatus/means that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, article, or apparatus/means.
Thus far, the technical solution of the present invention has been described in connection with the preferred embodiments shown in the drawings, but it is easily understood by those skilled in the art that the scope of protection of the present invention is not limited to these specific embodiments. Equivalent modifications and substitutions for related technical features may be made by those skilled in the art without departing from the principles of the present invention, and such modifications and substitutions will fall within the scope of the present invention.
Claims (10)
1. The utility model provides a separator with cytoprotection function, its characterized in that includes separator box (1), separation motor (3), seal box (5), goes out liquid box (7), collection box (9) and sealed chamber (12), the inside rotation of separator box (1) is connected with separating drum (2), the bottom of separating drum (2) is connected with the output of separation motor (3), go out the liquid hole on the outer wall of separating drum (2) bottom, go out the inside sliding connection in liquid hole has sealing piece (4), go out liquid hole and sealing box (5) one end sealing connection and fluid conduction, sealing box (5) inside sliding connection has centrifugal piece (6), sealing box (5) other end and go out liquid box (7) one end sealing connection and fluid conduction, go out liquid box (7) inside sliding connection has sealing shell (8), sealing shell (8) with one end of sealing box (5) supports the top, go out liquid box (7) other end and collection box (9) one end sealing connection and fluid conduction, sealing box (9) are installed on two top (10) are in order to separate the top of two classification boxes (1), the sealing cover (11) is in sealing connection with the sealing cavity (12), and the sealing cavity (12) is positioned on the inner side wall of the separation box (1).
2. The separator with a cytoprotective function according to claim 1, characterized in that friction lines are formed on the side wall of the sealing block (4), the top of the sealing block (4) is fixedly connected with one end of a sealing spring (13), the other end of the sealing spring (13) is fixedly connected with the inner wall of the separating cylinder (2), the outer wall of the sealing block (4) is fixedly connected with the outer wall of a pressing rod (14), and the bottom of the pressing rod (14) is slidably connected inside the sealing box (5).
3. The separator with a cytoprotective function according to claim 2, characterized in that a baffle plate (15) is fixedly installed in the sealing box (5), a sealing groove is formed in the inner wall of the baffle plate (15), one end of the centrifugal block (6) is fixedly connected with one end of a return spring (16), the other end of the return spring (16) is fixedly connected with the inner wall of the sealing box (5), friction lines are formed in the side wall of the centrifugal block (6), and a driving toothed plate (17) is fixedly installed on the outer wall of one end, far away from the return spring (16), of the centrifugal block (6).
4. A separator with cytoprotective function according to claim 3, characterized in that the outlet end of the sealing box (5) is slidingly connected with a sliding box (18), the inside of the side wall of the sliding box (18) is rotationally connected with a driving gear (19), the outer wall of the driving gear (19) is meshed with a driving toothed plate (17), a fixed toothed plate (20) is fixedly arranged on the outer wall of the top of the sliding box (18), and the fixed toothed plate (20) is meshed with the driving gear (19).
5. The separator with a cytoprotective function according to claim 4, wherein a sliding plate (21) is slidably connected to a side wall of the liquid outlet box (7), one end of the sliding plate (21) located outside the liquid outlet box (7) abuts against an outer wall of the sliding box (18), the other end of the sliding plate (21) is set to be an inclined plane and abuts against a top of a pressing block (22), the width of the pressing block (22) is reduced from top to bottom, the bottom of the pressing rod (14) is located on the side wall of one end of the sliding plate (21), a speed limiting plate (23) is mounted on the outer wall of the liquid outlet box (7), and the speed limiting plate (23) abuts against the outer wall of the speed limiting sealing box (5).
6. The separator with the cytoprotective function according to claim 1, wherein a separator plate (24) is fixedly installed in the collecting box (9), a liquid leakage hole is formed in the inner wall of the separator plate (24), the liquid leakage hole is in sealing connection with the top of the classifying box (10) and is in fluid conduction, a flow changing plate (25) is fixedly installed at the top of the separator plate (24), self-adjusting blocks (26) are installed in the inner wall of the liquid leakage hole, and the number of the self-adjusting blocks (26) is two or more.
7. The separator with cytoprotection function according to claim 6, characterized in that, sliding connection has gag lever post (27) on the inner wall of classifying box (10), install flexible closing plate on the outer wall of gag lever post (27), gag lever post (27) are located the bottom fixed mounting of the inside one end of classifying box (10) has cardboard (28), the inside sliding connection of classifying box (10) has floating plate (29), fixed mounting has card case (30) on the outer wall of floating plate (29) one end, the inside of card case (30) and the one end fixed connection of drive line (31), the other end and the outer wall fixed connection of flexible axis of rotation (32) of drive line (31), flexible axis of rotation (32) rotate to connect at the top of floating plate (29), the top of flexible axis of rotation (32) rotates to connect in the inside of self-adjusting piece (26).
8. The separator with a cytoprotection function according to claim 7, characterized in that an abutment block (33) is fixedly installed on the outer wall of the top of the telescopic rotating shaft (32), the abutment block (33) abuts against one end of an abutment rod (34), the other end of the abutment rod (34) is fixedly connected with one end of a filling plate (35), the filling plate (35) is slidably connected inside the self-adjusting block (26), the outer wall of the abutment rod (34) is fixedly connected with one end of a connecting wire (36), the other end of the connecting wire (36) is fixedly connected with the outer wall of the abutment block (33), and scales (37) are arranged on the outer wall of the classifying box (10).
9. The separator with the cytoprotection function according to claim 1, characterized in that a driving shaft (38) is rotatably connected in the separating box (1), one end of the driving shaft (38) is in driving connection with the output end of the separating motor (3), a sliding shaft (39) is slidably connected on the outer wall of the other end of the driving shaft (38), the sliding shaft (39) is slidably connected on the inner wall of the separating box (1), a rotating gear (40) is mounted on the outer wall of the sliding shaft (39), the rotating gear (40) is in meshing connection with a driven gear (41), the driven gear (41) is mounted on the outer wall of a driving screw (42), the driving screw (42) is rotatably connected in the sealing cavity (12), a pushing plate (43) is in threaded connection on the outer wall of the driving screw (42), and threads on the two ends of the driving screw (42) are opposite.
10. The separator with cytoprotective function according to claim 9, characterized in that the bottom of the sealing cover (11) is fixedly provided with a sealing plate (44), and the sealing plate (44) is clamped inside the sealing cavity (12).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311037821.8A CN116751662B (en) | 2023-08-17 | 2023-08-17 | Separator with cytoprotection function |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311037821.8A CN116751662B (en) | 2023-08-17 | 2023-08-17 | Separator with cytoprotection function |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN116751662A true CN116751662A (en) | 2023-09-15 |
| CN116751662B CN116751662B (en) | 2023-11-17 |
Family
ID=87951819
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311037821.8A Active CN116751662B (en) | 2023-08-17 | 2023-08-17 | Separator with cytoprotection function |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116751662B (en) |
Citations (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1068657A (en) * | 1976-05-14 | 1979-12-25 | Herbert M. Cullis | Disposable centrifugal blood processing system |
| US4879031A (en) * | 1987-04-07 | 1989-11-07 | Dideco S.P.A. | Blood centrifugation cell |
| JPH022884A (en) * | 1988-06-13 | 1990-01-08 | Hitachi Ltd | Centrifugal separator for biological cell and separation of the same cell |
| US5171532A (en) * | 1990-02-23 | 1992-12-15 | Eastman Kodak Company | Centrifuge-containing analyzer |
| JPH0690737A (en) * | 1992-09-14 | 1994-04-05 | Hitachi Ltd | Centrifugal separation apparatus for biocell and cell cultivation method |
| US20020147098A1 (en) * | 2001-04-09 | 2002-10-10 | Dolecek Victor D. | Method for handling blood sample to ensure blood components are isolated |
| US20030176267A1 (en) * | 2000-12-29 | 2003-09-18 | Gunter Eberle | Centrifuge comprising a blood bag system with an upper and lower outlet |
| US20080014181A1 (en) * | 2002-03-29 | 2008-01-17 | Ariff Gregory D | Cell separation apparatus and methods of use |
| CN109988706A (en) * | 2017-12-29 | 2019-07-09 | 肖锷 | A kind of centrifugal bottle and stem cell are classified extraction element |
| CN110448751A (en) * | 2019-07-26 | 2019-11-15 | 北京中科盛康科技有限公司 | A kind of Autoblood recovery device |
| CN112574854A (en) * | 2020-12-21 | 2021-03-30 | 上饶市春广科技有限公司 | Cell separation device with biological cell tissue protection function |
| US20210205734A1 (en) * | 2019-06-06 | 2021-07-08 | Pneumatic Scale Corporation | Centrifuge System for Separating Cells in Suspension |
| CN114085769A (en) * | 2021-11-17 | 2022-02-25 | 郭兴华 | Stem cell is drawed with centrifugal intelligence extraction system |
| CN216444094U (en) * | 2021-10-28 | 2022-05-06 | 河南睿英生物技术有限公司 | Efficient T lymphocyte separation kit |
| CN114849911A (en) * | 2022-05-19 | 2022-08-05 | 梁靓 | Blood centrifugal separation equipment |
| CN116179340A (en) * | 2023-03-02 | 2023-05-30 | 中南大学湘雅医院 | Cell multilayer separation device |
| CN116689166A (en) * | 2023-08-07 | 2023-09-05 | 深圳美讯医学检验科技有限公司 | Plasma quick separation preparation facilities |
-
2023
- 2023-08-17 CN CN202311037821.8A patent/CN116751662B/en active Active
Patent Citations (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1068657A (en) * | 1976-05-14 | 1979-12-25 | Herbert M. Cullis | Disposable centrifugal blood processing system |
| US4879031A (en) * | 1987-04-07 | 1989-11-07 | Dideco S.P.A. | Blood centrifugation cell |
| JPH022884A (en) * | 1988-06-13 | 1990-01-08 | Hitachi Ltd | Centrifugal separator for biological cell and separation of the same cell |
| US5171532A (en) * | 1990-02-23 | 1992-12-15 | Eastman Kodak Company | Centrifuge-containing analyzer |
| JPH0690737A (en) * | 1992-09-14 | 1994-04-05 | Hitachi Ltd | Centrifugal separation apparatus for biocell and cell cultivation method |
| US20030176267A1 (en) * | 2000-12-29 | 2003-09-18 | Gunter Eberle | Centrifuge comprising a blood bag system with an upper and lower outlet |
| US20020147098A1 (en) * | 2001-04-09 | 2002-10-10 | Dolecek Victor D. | Method for handling blood sample to ensure blood components are isolated |
| US20080014181A1 (en) * | 2002-03-29 | 2008-01-17 | Ariff Gregory D | Cell separation apparatus and methods of use |
| CN109988706A (en) * | 2017-12-29 | 2019-07-09 | 肖锷 | A kind of centrifugal bottle and stem cell are classified extraction element |
| US20210205734A1 (en) * | 2019-06-06 | 2021-07-08 | Pneumatic Scale Corporation | Centrifuge System for Separating Cells in Suspension |
| CN110448751A (en) * | 2019-07-26 | 2019-11-15 | 北京中科盛康科技有限公司 | A kind of Autoblood recovery device |
| CN112574854A (en) * | 2020-12-21 | 2021-03-30 | 上饶市春广科技有限公司 | Cell separation device with biological cell tissue protection function |
| CN216444094U (en) * | 2021-10-28 | 2022-05-06 | 河南睿英生物技术有限公司 | Efficient T lymphocyte separation kit |
| CN114085769A (en) * | 2021-11-17 | 2022-02-25 | 郭兴华 | Stem cell is drawed with centrifugal intelligence extraction system |
| CN114849911A (en) * | 2022-05-19 | 2022-08-05 | 梁靓 | Blood centrifugal separation equipment |
| CN116179340A (en) * | 2023-03-02 | 2023-05-30 | 中南大学湘雅医院 | Cell multilayer separation device |
| CN116689166A (en) * | 2023-08-07 | 2023-09-05 | 深圳美讯医学检验科技有限公司 | Plasma quick separation preparation facilities |
Non-Patent Citations (1)
| Title |
|---|
| 金绿松;: "自抽连续离心技术的研究――5.自抽连续液/固离心分离机的研制", 长春理工大学学报, no. 01 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN116751662B (en) | 2023-11-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN2938235Y (en) | Coin sorting apparatus | |
| CN212110851U (en) | Centrifugal separation device for food detection | |
| CN116751662B (en) | Separator with cytoprotection function | |
| CN115711769A (en) | Chemical industry detects uses quick sampling equipment | |
| CN112557239A (en) | Device for detecting precipitation amount of dissolved biological capsules | |
| CN112903366A (en) | Stratified granary sampler capable of avoiding grain flowing | |
| CN114659838A (en) | Hydrological monitoring water quality sampling ship and using method thereof | |
| CN111232610A (en) | Unpowered automatic positive and negative cover removing device | |
| CN114699319A (en) | Full-automatic enteric hollow capsule medicine continuous filling equipment | |
| CN219545243U (en) | Automatic packaging system | |
| US4181700A (en) | Centrifuge tube sequential fractionator | |
| CN113074985B (en) | Water sample classification sampling device for ocean management | |
| CN110917656A (en) | Chemical experiment extraction device | |
| CN218056636U (en) | Tealeaves packing carton convenient to use | |
| CN116819015A (en) | Food detection device for food production | |
| CN111746913B (en) | Biological reagent detect reagent box | |
| CN114217083A (en) | Reagent instillation device for medical examination | |
| CN113933469A (en) | Multi-functional food safety inspection uses device | |
| CN208855900U (en) | A kind of empty bag rejecting mechanism | |
| CN111337329A (en) | Automatic salt adding device for salt powder tube sample pretreatment | |
| CN219348376U (en) | Blood sample impurity filtering device for blood detection | |
| CN111904866A (en) | Intelligent medicine box capable of realizing capsule counting and medicine discharging | |
| CN220310706U (en) | Centrifugal machine for walnut oil detection sampling | |
| CN218930299U (en) | Blood glucose test paper pops out box | |
| CN215115335U (en) | Sample collection system for lubricating oil production |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |