CN116660547A - Specific protein analysis and detection device with automatic sample adding function - Google Patents
Specific protein analysis and detection device with automatic sample adding function Download PDFInfo
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- CN116660547A CN116660547A CN202310640591.8A CN202310640591A CN116660547A CN 116660547 A CN116660547 A CN 116660547A CN 202310640591 A CN202310640591 A CN 202310640591A CN 116660547 A CN116660547 A CN 116660547A
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- 238000001514 detection method Methods 0.000 title claims abstract description 61
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 25
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 25
- 238000004140 cleaning Methods 0.000 claims abstract description 113
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 47
- 238000002347 injection Methods 0.000 claims abstract description 47
- 239000007924 injection Substances 0.000 claims abstract description 47
- 238000001035 drying Methods 0.000 claims abstract description 31
- 239000000243 solution Substances 0.000 claims abstract description 26
- 239000012460 protein solution Substances 0.000 claims abstract description 19
- 230000000712 assembly Effects 0.000 claims abstract description 10
- 238000000429 assembly Methods 0.000 claims abstract description 10
- 238000002331 protein detection Methods 0.000 claims abstract description 7
- 238000004891 communication Methods 0.000 claims description 27
- 238000001125 extrusion Methods 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 238000005485 electric heating Methods 0.000 claims description 3
- 238000000034 method Methods 0.000 claims 1
- 238000004804 winding Methods 0.000 description 15
- 239000007788 liquid Substances 0.000 description 7
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 4
- 235000017491 Bambusa tulda Nutrition 0.000 description 4
- 241001330002 Bambuseae Species 0.000 description 4
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 4
- 239000011425 bamboo Substances 0.000 description 4
- 230000005540 biological transmission Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000005611 electricity Effects 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000000862 absorption spectrum Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1002—Reagent dispensers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1004—Cleaning sample transfer devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N2035/1027—General features of the devices
- G01N2035/1048—General features of the devices using the transfer device for another function
- G01N2035/1058—General features of the devices using the transfer device for another function for mixing
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a specific protein analysis and detection device with an automatic sample adding function, and relates to the technical field of protein detection; the system comprises a system host, wherein a detection host is arranged on the side surface of the system host, and a top cylinder is arranged on the detection host in a sliding manner; a clamping assembly is arranged in the detection host; a cleaning collecting box is arranged at the top of the top cylinder, and a cleaning cavity and a collecting cavity are arranged in the cleaning collecting box; the top of the top cylinder is also provided with a solution cylinder and a reagent cylinder, and the reagent cylinder and the solution cylinder are respectively communicated with the cleaning cavity; the top cylinder is also provided with a cleaning component and a drying component for cleaning the sample tube; two injection assemblies are arranged in the top cylinder and are used for injecting protein solution and reagent; the invention can realize the automatic addition of the sample, fully clean the sample tube, and completely clean the path for extracting the sample without influencing the subsequent use.
Description
Technical Field
The invention relates to the technical field of protein detection, in particular to a specific protein analysis and detection device with an automatic sample adding function.
Background
Proteins are the material basis of life, are organic macromolecules, are basic organic matters constituting cells, and amino acids are the basic constituent units of proteins; it is a substance that is closely linked to life and to various forms of life activities. Every cell and all important components in the body are protein-involved; proteins in human bodies are various in variety, different in property and function, but are formed by combining more than 20 amino acids according to different proportions, and are continuously metabolized and updated in the body.
Protein analysis can be used to determine the molecular weight, structure, composition and function of proteins, and can also be used to find and identify modifications and other compounds in proteins; the existing protein analysis mode is generally to carry out analysis and detection through a protein detection instrument, namely, different substances have different absorption capacities on light with different wavelengths due to different molecular structures by utilizing a spectrophotometry, so that the protein analysis mode has a special absorption spectrum; however, before analyzing proteins, the protein solution needs to be grouped and placed in an instrument, the flow is complicated, and a container for containing the solution needs to be cleaned later, so that the efficiency of the whole detection flow is low; for example, chinese patent publication No. CN109324200B discloses a sample analyzer, which has the following technical scheme: a host and a sample injector separate from the host; the host comprises a sampling arm, an incubation plate measuring device and a reagent supply device, wherein the sampling arm absorbs reagents and samples from the reagent supply device and a sampling station, and then the reagents and the samples are injected into a reaction cup arranged in the incubation plate measuring device for incubation test; from the technical scheme, the patent mainly solves the problem of how to transport and supply the sample, namely, the automatic adding function of the sample and the reagent is realized; in the description, the stirring mechanism 3b provided in this patent can drive the reagent needle to stir the reagent, but the reagent remains on the reagent needle after stirring, which affects the subsequent use.
Disclosure of Invention
Aiming at the technical problems, the invention can realize automatic addition of the sample, fully clean the sample tube, and completely clean the path for extracting the sample without influencing the subsequent use.
The technical scheme adopted by the invention is as follows: the specific protein analysis and detection device with the automatic sample adding function comprises a system host, wherein a detection host is arranged on the side face of the system host, a top cylinder is arranged on the detection host in a sliding mode, detection cavities which are arranged in a circular array are arranged on the detection host, and sample tubes are arranged in the detection cavities for protein detection; the clamping assembly is arranged in the detection host and used for clamping the sample tube, and comprises a clamping plate I fixedly arranged on the inner side of the detection host and a clamping plate II slidably arranged in the detection host, and the sample tube is clamped through the clamping plate I and the clamping plate II; a vibration supporting frame is arranged at the bottom of the detection host in a sliding manner and is used for supporting the sample tube and vibrating the sample tube so as to mix the protein solution and the reagent; a turntable is rotatably arranged at the top of the top cylinder, a cleaning collecting box is arranged on the turntable, and a cleaning cavity and a collecting cavity are arranged in the cleaning collecting box; the turntable is provided with a solution cylinder for containing a protein solution to be detected and a reagent cylinder for containing a reagent, and the reagent cylinder and the solution cylinder are respectively communicated with the cleaning cavity; the rotary table is also provided with a cleaning component for cleaning the sample tube and a drying component for drying the sample tube, the cleaning component comprises a valve seat which is arranged on the rotary table in a sliding way, the valve seat is respectively communicated with the cleaning cavity and the collecting cavity, the valve seat is provided with a first connecting pipe, one end of the first connecting pipe is rotatably provided with a cleaning cylinder, and the circumference of the cleaning cylinder is provided with a water outlet; the drying assembly comprises a second connecting pipe which is arranged on the turntable in a sliding manner, and a drying cylinder is rotatably arranged at one end of the second connecting pipe; two injection assemblies are arranged in the top cylinder and are respectively communicated with the solution cylinder and the reagent cylinder, the injection assemblies comprise quantitative cylinders for quantitative, and injection pipes are movably arranged at the bottom ends of the quantitative cylinders; a communication box is arranged in the detection host, two matching components are arranged on the outer side of the communication box, and each matching component comprises a matching pipe which is arranged on the outer side of the communication box in a sliding manner and communicated with the communication box; the communicating box is communicated with a collecting and releasing pipe, one end of the collecting and releasing pipe is communicated with the collecting cavity, and one end of the injection pipe is matched with the matching pipe to form a cleaning loop.
Further, the clamping assembly comprises an extrusion ring rotatably arranged in the detection host, an extrusion lug is arranged on the circumference of the extrusion ring, one end of the second clamping plate is in contact extrusion relation with the extrusion lug, and a clamping spring used for providing elasticity is connected between the second clamping plate and the detection host.
Further, a bracket is arranged in the detection host, a motor III is arranged on the bracket, a gear III is connected to an output shaft of the motor III, and a gear pair is formed by the gear III and the inner side of the extrusion ring; the communication case sets up on the support, still is provided with the lift jar in the inside of detecting the host computer, and the lift jar is used for controlling vibrations support frame and slides.
Further, the vibration support frame comprises a support plate, a vibration motor is arranged on the support plate, a support seat is connected to the support plate, a vibration plate is movably arranged on the support seat, and a support spring is connected between the vibration plate and the support seat.
Further, a winding and unwinding cylinder is rotatably arranged on the communication box, the winding and unwinding cylinder is of a hollow structure, one end of the winding and unwinding cylinder is communicated with the communication box, the winding and unwinding pipe is communicated with the winding and unwinding cylinder and is wound on the winding and unwinding cylinder, and a winding and unwinding motor for driving the winding and unwinding cylinder to rotate is arranged on the communication box.
Further, the cooperation subassembly is including setting up the cooperation cylinder in the intercommunication case outside, and the telescopic link and the cooperation pipe fixed connection of cooperation cylinder to drive the cooperation pipe and slide.
Further, a guide rod is fixedly arranged on the outer side of the detection host, a lifting screw rod is rotatably arranged on the outer side of the detection host, and a screw rod motor for driving the lifting screw rod to rotate is arranged on the top of the lifting screw rod; the top cylinder is in sliding connection with the guide rod and forms a spiral pair with the lifting screw rod; the top of the guide rod is provided with a second motor, an output shaft of the second motor is connected with a second gear, and the second gear and the turntable form a gear pair.
Further, the cleaning assembly comprises a first cleaning motor arranged on the turntable and a first cleaning screw rod rotatably arranged on the turntable, the first cleaning motor is used for driving the first cleaning screw rod to rotate, a first sliding seat is slidably arranged on the turntable, the first sliding seat and the first cleaning screw rod form a spiral pair, and the valve seat is fixedly arranged on the first sliding seat; one end of the cleaning cylinder is connected with a first meshing gear; the drying assembly comprises a second cleaning motor arranged on the turntable and a second cleaning screw rod rotatably arranged on the turntable, wherein the second cleaning motor is used for driving the second cleaning screw rod to rotate; the rotary table is provided with a second sliding seat in a sliding manner, the second sliding seat and the second cleaning screw rod form a spiral pair, the second connecting pipe is fixedly connected to the second sliding seat, one end of the drying cylinder is connected with a second meshing gear, and an electric heating wire is arranged in the drying cylinder; the inner side of the top cylinder is rotatably provided with a gear ring, the outer side of the top cylinder is provided with a first motor, an output shaft of the first motor is connected with a gear I which forms a gear pair with the gear ring, and when the meshing gear I and the meshing gear II are respectively meshed with the gear ring, the cleaning cylinder and the drying cylinder are driven to rotate through the gear ring.
Further, injection subassembly is including setting up the position electricity jar on the carousel, is connected with the connecting rod on the telescopic link of position electricity jar, and the one end and the injection tube fixed connection of connecting rod are provided with the ration cylinder on the carousel, are connected with the piston board on the telescopic link of ration cylinder, and the piston board is in inboard sliding connection with the ration section of thick bamboo.
Further, the reagent cylinder and the solution cylinder are respectively communicated with the cleaning cavity through a first pipeline, and the valve seat is respectively communicated with the cleaning cavity and the collecting cavity through a second pipeline; the quantitative cylinders on the two injection assemblies are respectively communicated with the reagent cylinder and the solution cylinder through a pipeline III, and the injection tube is communicated with the quantitative cylinder through a pipeline IV.
Compared with the prior art, the invention has the beneficial effects that: (1) According to the invention, the protein solution and the reagent can be automatically injected into the sample tubes in all directions through the injection assembly, the quantitative cylinder is used for quantitative determination, the amount of the protein solution and the reagent which should be injected into each sample tube is determined, and the sample tubes are vibrated through vibration, so that the mixing of the protein solution and the reagent is realized; (2) The cleaning assembly is used for repeatedly sucking the solution in the sample tube for multiple times to achieve the cleaning effect, namely the solution is sucked away through the water outlet hole on the cleaning cylinder and flows into the collecting cavity, and then cleaning liquid is introduced into the sample tube through the cleaning chamber to clean the sample tube in multiple stages; drying the sample tube through a drying component to remove residual liquid; (3) The invention can clean the injection path of the protein solution and the reagent, and is matched with the injection port of the injection tube through the matching tube; forming a cleaning loop; when cleaning the injection route, carry out the cleaning solution transmission through clean chamber, the cleaning solution is through pipeline one, enters into reagent section of thick bamboo and solution section of thick bamboo, then through two injection components, namely pipeline three, quantitative cylinder, pipeline four, injection tube, cooperation pipe and communication case, then transmit to receive and release section of thick bamboo, receive and release pipe through the communication case, finally to collecting the chamber, pump repeatedly, accomplish the cleanness of injection route, do not influence follow-up detection.
Drawings
FIG. 1 is a schematic diagram of the overall structure of the present invention.
FIG. 2 is a schematic view of another angle of the overall structure of the present invention.
Fig. 3 is a top view of the overall structure of the present invention.
Fig. 4 is a schematic view of a clamping assembly according to the present invention.
Fig. 5 is an enlarged view of a portion of the invention at the a-mark in fig. 4.
FIG. 6 is a schematic diagram of a portion of the inside of the inspection host according to the present invention.
Fig. 7 is a cross-sectional view of a test host according to the present invention.
Fig. 8 is a schematic view of a vibration supporting frame according to the present invention.
Fig. 9 is a cross-sectional view of the top cartridge of the present invention.
Fig. 10 is a schematic view of an injection assembly of the present invention.
Reference numerals: 1-a system host; 2-detecting a host; 3-motor one; 4-gear one; 5-pushing a cylinder; 6-a turntable; 7-a reagent cartridge; 8-a solution cylinder; 9, cleaning a collecting box; 10-a second motor; 11-gear II; 12-a guide rod; 13-a screw motor; 14-lifting screw rods; 15-first pipeline; 16-a second pipeline; 17-clamping plate I; 18-a second clamping plate; 19-clamping a spring; 20-extruding rings; 21-a communication box; 22-winding and unwinding motors; 23-a retractable cylinder; 24-winding and unwinding the pipe; 25-mating the cylinder; 26-fitting the tube; 27-gear three; 28-motor three; 29-a bracket; 30-lifting electric cylinders; 31-supporting plates; 32-a supporting seat; 33-supporting springs; 34-vibrating plate; 35-a vibration motor; 36-cleaning a first motor; 37-cleaning a first screw rod; 38-first slide; 39-valve seat; 40-connecting the pipe I; 41-meshing gear one; 42-cleaning cylinder; 43-ring gear; 44-cleaning a second motor; 45-cleaning a second screw rod; 46-a second slide seat; 47-connecting the pipe II; 48-meshing gear II; 49-heating wire; 50-a drying cylinder; 51-conduit three; 52-a quantitative cylinder; 53-a metering cylinder; 54-position electric cylinder; 55-connecting rods; 56-a syringe; 57-pipe four.
Detailed Description
The invention is described in detail below with reference to the attached drawings: as shown in fig. 1 to 3, a specific protein analysis and detection device with an automatic sample adding function comprises a system host 1, wherein a detection host 2 is arranged on one side of the system host 1, detection cavities which are arranged in a circular array are arranged on the detection host 2, and sample tubes are arranged in the detection cavities for protein detection; the detection host 2 is fixedly connected with a guide rod 12 and is rotatably provided with a lifting screw rod 14, the top end of the lifting screw rod 14 is provided with a screw rod motor 13 for driving the lifting screw rod 14 to rotate, the guide rod 12 is slidably provided with a top cylinder 5, and the top cylinder 5 and the lifting screw rod 14 form a screw pair; a rotary table 6 is rotatably arranged on the top cylinder 5, a second motor 10 is arranged at the top end of the guide rod 12, a second gear 11 is connected to an output shaft of the second motor 10, and the second gear 11 and the rotary table 6 form a gear pair to drive the rotary table 6 to rotate; a cleaning collecting box 9 is arranged on the turntable 6, and a collecting cavity and a cleaning cavity are arranged inside the cleaning collecting box 9.
A solution cylinder 8 for containing a protein solution to be detected and a reagent cylinder 7 for containing a reagent are arranged on the turntable 6, the reagent cylinder 7 and the solution cylinder 8 are respectively communicated with the cleaning cavity through a first pipeline 15, and a first valve pump is arranged at one end of the first pipeline 15; a motor I3 is arranged on the outer side of the top cylinder 5, and a gear I4 is connected to an output shaft of the motor I3; as shown in fig. 9, a ring gear 43 is rotatably mounted on the inner side of the top tube 5, and the ring gear 43 and the first gear 4 constitute a gear pair.
As shown in fig. 9, a cleaning assembly for cleaning the sample tube and a drying assembly for drying the sample tube are provided on the turntable 6; the drying assembly comprises a first cleaning motor 36 arranged on the turntable 6 and a first cleaning screw 37 rotatably arranged on the turntable 6, wherein the first cleaning motor 36 is used for driving the first cleaning screw 37 to rotate; a first sliding seat 38 is slidably arranged on the turntable 6, a valve seat 39 is fixedly arranged on the first sliding seat 38, and the first sliding seat 38 and the first cleaning screw rod 37 form a screw pair; the valve seat 39 is provided with a first connecting pipe 40, one end of the first connecting pipe 40 rotates and is communicated with a cleaning cylinder 42, one end of the cleaning cylinder 42 is connected with a first meshing gear 41, water outlet holes are arranged on the circumference of the cleaning cylinder 42, and when the first meshing gear 41 is meshed with a gear ring 43, the cleaning cylinder 42 is driven to rotate through the gear ring 43; the drying assembly comprises a second cleaning motor 44 arranged on the turntable 6 and a second cleaning screw rod 45 rotatably arranged on the turntable 6, the second cleaning motor 44 is used for driving the second cleaning screw rod 45 to rotate, a second sliding seat 46 is slidably arranged on the turntable 6, the second sliding seat 46 and the second cleaning screw rod 45 form a spiral pair, a second connecting pipe 47 is fixedly connected to the second sliding seat 46, one end of the second connecting pipe 47 is rotatably provided with a drying cylinder 50, one end of the drying cylinder 50 is connected with a second meshing gear 48, and an electric heating wire 49 is arranged in the drying cylinder 50, and when the second meshing gear 48 is meshed with the gear ring 43, the drying cylinder 50 is driven to rotate through the gear ring 43; as shown in fig. 3, the valve seat 39 is respectively communicated with the cleaning cavity and the collecting cavity through a second pipeline 16, and a second valve pump is arranged at one end of the second pipeline 16.
As shown in fig. 9 to 10, two injection assemblies, one for injecting a protein solution to be detected and the other for injecting a reagent, are provided inside the top cartridge 5; the injection assembly comprises a quantifying cylinder 53 and a quantifying cylinder 52 which are arranged on the turntable 6, a piston plate is connected to a telescopic rod of the quantifying cylinder 52, and the piston plate is in sliding connection with the inner side of the quantifying cylinder 53; the rotary table 6 is also provided with a position electric cylinder 54, a telescopic rod of the position electric cylinder 54 is connected with a connecting rod 55, one end of the connecting rod 55 is connected with an injection tube 56, injection is carried out through the injection tube 56, one end of the injection tube 56 is communicated with the quantitative cylinder 53 through a pipeline IV 57, and one end of the pipeline IV 57 is provided with a valve pump III; the quantitative barrels 53 on the two injection assemblies are respectively communicated with the reagent barrel 7 and the solution barrel 8 through a third pipeline 51, and a valve pump IV is arranged at one end of the third pipeline 51.
As shown in fig. 4 to 6, a clamping assembly is arranged in the detection host 2, the clamping assembly comprises a first clamping plate 17 fixedly connected to the inner side of the detection host 2 and a second clamping plate 18 slidably mounted in the detection host 2, a clamping spring 19 which is used for providing elasticity is connected between the second clamping plate 18 and the detection host 2, a squeezing ring 20 is rotatably mounted in the detection host 2, a squeezing convex block is arranged on the circumference of the squeezing ring 20, and one end of the second clamping plate 18 is in contact squeezing relation with the squeezing convex block; the inside of the detection host 2 is connected with a support 29, a third motor 28 is arranged on the support 29, a third gear 27 is connected to an output shaft of the third motor 28, and the third gear 27 and the inner side of the extrusion ring 20 form a gear pair.
The support 29 is provided with a communication box 21, two matching components are arranged on the outer side of the communication box 21, each matching component comprises a matching cylinder 25 arranged on the outer side of the communication box 21, a matching pipe 26 is connected to a telescopic rod of each matching cylinder 25, one end of each matching pipe 26 is communicated with the communication box 21, a collecting and releasing cylinder 23 is rotatably arranged on the communication box 21, a collecting and releasing motor 22 for driving the collecting and releasing cylinder 23 to rotate is arranged on the communication box 21, the collecting and releasing cylinder 23 is of a hollow structure, one end of the collecting and releasing cylinder 23 is communicated with the communication box 21, a collecting and releasing pipe 24 is communicated with the collecting and releasing cylinder 23 in a winding mode, and one end of the collecting and releasing pipe 24 is communicated with a collecting cavity; one end of the syringe 56 mates with the mating tube 26 to form a cleaning circuit.
As shown in fig. 7 and 8, a lifting electric cylinder 30 is arranged in the detection host 2, a vibration support frame is connected to a telescopic rod of the lifting electric cylinder 30, the vibration support frame comprises a support plate 31, a vibration motor 35 is arranged on the support plate 31, a support seat 32 is connected to the support plate 31, a vibration plate 34 is movably arranged on the support seat 32, a support spring 33 is connected between the vibration plate 34 and the support seat 32, a sample tube is supported through the vibration plate 34, and the sample tube is vibrated through the vibration plate 34, so that mixing of a protein solution and a reagent is realized.
Working principle: the protein solution to be detected and the reagent are respectively placed in a solution cylinder 8 and a reagent cylinder 7, a sample tube is placed in a detection cavity in a detection host 2, a third motor 28 drives a third gear 27 to rotate, under the transmission of the gear, an extrusion ring 20 rotates, an extrusion lug pushes a second clamping plate 18, and the sample tube is clamped and fixed through the second clamping plate 18 and the first clamping plate 17; the screw motor 13 works, the lifting screw 14 rotates to enable the top cylinder 5 to slide, the top cylinder 5 and the detection host 2 are spliced into a whole, and then the injection of the protein detection solution and the reagent is carried out.
When the protein solution and the reagent are injected, the position cylinder 54 controls the injection tube 56 to move above the sample tubes in all directions, and the rotary table 6 rotates under the transmission of the gear through the second motor 10 to change the directions so as to inject the protein solution and the reagent into the sample tubes in all directions; the quantification is performed by the quantifying cylinder 53, and the quantifying cylinder 52 controls the position of the piston plate, i.e., determines the amount of protein solution and reagent that should be injected per sample tube; then injecting the protein solution and the reagent through the injection tube 56; after the protein solution and the reagent are injected, the vibration motor 35 works, so that the support plate 31 vibrates, the vibration effect is transmitted to the sample tube through the vibration plate 34, the sample tube is vibrated, the mixing of the protein solution and the reagent is realized, and the mixed solution is detected in the detection cavity.
When the detection is finished, cleaning the sample tube, repeatedly sucking liquid in the sample tube through a cleaning assembly, namely, operating a first cleaning motor 36, rotating a first cleaning screw rod 37, sliding a first sliding seat 38 and a valve seat 39 to enable a cleaning cylinder 42 to enter the sample tube, meshing a first meshing gear 41 with a gear ring 43, sucking solution away through a water outlet hole in the cleaning cylinder 42 and flowing into a collecting cavity, then introducing cleaning liquid into the sample tube through a cleaning chamber, operating a first motor 3, rotating a first gear 4, rotating the gear ring 43 under gear transmission, rotating the cleaning cylinder 42 inside the sample tube, washing the inner wall of the sample tube, repeatedly pumping the cleaning liquid for a plurality of times, and performing multistage cleaning on the sample tube; when the sample tube is subjected to subsequent drying treatment, the second cleaning motor 44 is operated, the second cleaning screw rod 45 is rotated, the second sliding seat 46 and the second connecting pipe 47 slide, the drying cylinder 50 is led into the sample tube, the second meshing gear 48 is meshed with the gear ring 43, the heating wire 49 is heated, the sample tube is dried through the drying cylinder 50, and residual liquid is removed.
When cleaning the injection path, the position cylinder 54 controls the injection tube 56 to slide so that the injection port of the injection tube 56 coincides with the matching tube 26 in the vertical direction, and the matching cylinder 25 controls the matching tube 26 to slide so that the matching tube 26 matches with the injection port of the injection tube 56; forming a cleaning loop; it should be noted that, the winding and unwinding motor 22 controls the winding and unwinding drum 23 to rotate, and can wind and unwind the winding and unwinding tube 24 to control the extension length of the winding and unwinding tube 24; when the injection path is cleaned, cleaning liquid is transmitted through the cleaning cavity, enters the reagent cylinder 7 and the solution cylinder 8 through the first pipeline 15, then passes through two injection components, namely a third pipeline 51, a quantitative cylinder 53, a fourth pipeline 57, an injection tube 56, a matching tube 26 and a communication box 21, and then is transmitted to the collecting and releasing cylinder 23 and the collecting and releasing tube 24 through the communication box 21, finally reaches the collecting cavity, and is repeatedly pumped, so that the cleaning of the injection path is completed without influencing the subsequent detection; when the sample tube is taken out, the support plate 31 can be controlled to slide by the lifting cylinder 30, and the vibration plate 34 supports and pushes the sample tube so as to pick up the sample tube.
Claims (10)
1. The specific protein analysis and detection device with the automatic sample adding function comprises a system host machine (1), wherein a detection host machine (2) is arranged on the side surface of the system host machine (1), a top cylinder (5) is arranged on the detection host machine (2) in a sliding manner, detection cavities which are distributed in a circular array are arranged on the detection host machine (2), and sample tubes are arranged in the detection cavities for protein detection; the method is characterized in that: a clamping assembly is arranged in the detection host machine (2) and used for clamping the sample tube, and comprises a clamping plate I (17) fixedly arranged on the inner side of the detection host machine (2) and a clamping plate II (18) slidingly arranged in the detection host machine (2); a vibration supporting frame is arranged at the bottom of the detection host machine (2) in a sliding manner and is used for supporting a sample tube and vibrating the sample tube so as to mix a protein solution and a reagent; a rotary table (6) is rotatably arranged at the top of the top cylinder (5), a cleaning collecting box (9) is arranged on the rotary table (6), and a cleaning cavity and a collecting cavity are arranged in the cleaning collecting box (9); a solution cylinder (8) and a reagent cylinder (7) are arranged on the turntable (6), and the reagent cylinder (7) and the solution cylinder (8) are respectively communicated with the cleaning cavity; the rotary table (6) is also provided with a cleaning component for cleaning the sample tube and a drying component for drying the sample tube, the cleaning component comprises a valve seat (39) which is arranged on the rotary table (6) in a sliding manner, the valve seat (39) is respectively communicated with the cleaning cavity and the collecting cavity, the valve seat (39) is provided with a first connecting pipe (40), one end of the first connecting pipe (40) is rotatably provided with a cleaning cylinder (42), and the circumference of the cleaning cylinder (42) is provided with a water outlet; the drying assembly comprises a second connecting pipe (47) which is arranged on the turntable (6) in a sliding manner, and a drying cylinder (50) is rotatably arranged at one end of the second connecting pipe (47); two injection assemblies are arranged in the top cylinder (5), the two injection assemblies are respectively communicated with the solution cylinder (8) and the reagent cylinder (7), the injection assemblies comprise a quantitative cylinder (53) for quantitative, and an injection tube (56) is movably arranged at the bottom end of the quantitative cylinder (53); a communication box (21) is arranged in the detection host (2), two matching components are arranged on the outer side of the communication box (21), and each matching component comprises a matching pipe (26) which is arranged on the outer side of the communication box (21) in a sliding manner and communicated with the communication box (21); a collecting and releasing pipe (24) is communicated with the communication box (21), one end of the collecting and releasing pipe (24) is communicated with the collecting cavity, and when one end of the injection pipe (56) is matched with the matching pipe (26), a cleaning loop is formed.
2. The apparatus for analyzing and detecting a specific protein with an automatic sample addition function according to claim 1, wherein: the clamping assembly comprises an extrusion ring (20) rotatably arranged in the detection host machine (2), an extrusion lug is arranged on the circumference of the extrusion ring (20), one end of a clamping plate II (18) is in contact extrusion relation with the extrusion lug, and a clamping spring (19) for providing elasticity is connected between the clamping plate II (18) and the detection host machine (2).
3. The apparatus for analyzing and detecting a specific protein with an automatic sample addition function according to claim 2, wherein: a bracket (29) is arranged in the detection host (2), a motor III (28) is arranged on the bracket (29), a gear III (27) is connected to an output shaft of the motor III (28), and the gear III (27) and the inner side of the extrusion ring (20) form a gear pair; the communication box (21) is arranged on the support (29), and a lifting electric cylinder (30) is further arranged in the detection host (2), and the lifting electric cylinder (30) is used for controlling the vibration support frame to slide.
4. The apparatus for analyzing and detecting a specific protein with an automatic sample addition function according to claim 3, wherein: the vibration support frame comprises a support plate (31), a vibration motor (35) is arranged on the support plate (31), a support seat (32) is connected to the support plate (31), a vibration plate (34) is movably arranged on the support seat (32), and a support spring (33) is connected between the vibration plate (34) and the support seat (32).
5. The apparatus for analyzing and detecting a specific protein with an automatic sample addition function according to claim 3, wherein: the novel retractable tube is characterized in that a retractable tube (23) is rotatably arranged on the communicating box (21), the retractable tube (23) is of a hollow structure, one end of the retractable tube (23) is communicated with the communicating box (21), a retractable tube (24) is communicated with the retractable tube (23) and is wound on the retractable tube (23), and a retractable motor (22) for driving the retractable tube (23) to rotate is arranged on the communicating box (21).
6. The apparatus for analyzing and detecting a specific protein with an automatic sample addition function according to claim 1, wherein: the matching component comprises a matching air cylinder (25) arranged on the outer side of the communication box (21), and a telescopic rod of the matching air cylinder (25) is fixedly connected with the matching pipe (26) so as to drive the matching pipe (26) to slide.
7. The apparatus for analyzing and detecting a specific protein with an automatic sample addition function according to claim 1, wherein: the outer side of the detection host machine (2) is fixedly provided with a guide rod (12), a lifting screw rod (14) is rotatably arranged, and a screw rod motor (13) for driving the lifting screw rod (14) to rotate is arranged at the top of the lifting screw rod (14); the top cylinder (5) is in sliding connection with the guide rod (12) and forms a screw pair with the lifting screw rod (14); the top of the guide rod (12) is provided with a second motor (10), an output shaft of the second motor (10) is connected with a second gear (11), and the second gear (11) and the rotary table (6) form a gear pair.
8. The apparatus for analyzing and detecting a specific protein with an automatic sample addition function according to claim 1, wherein: the cleaning assembly comprises a first cleaning motor (36) arranged on the rotary table (6) and a first cleaning screw rod (37) rotatably arranged on the rotary table (6), the first cleaning motor (36) is used for driving the first cleaning screw rod (37) to rotate, a first sliding seat (38) is slidably arranged on the rotary table (6), the first sliding seat (38) and the first cleaning screw rod (37) form a screw pair, and a valve seat (39) is fixedly arranged on the first sliding seat (38); one end of the cleaning cylinder (42) is connected with a meshing gear I (41); the drying assembly comprises a second cleaning motor (44) arranged on the turntable (6) and a second cleaning screw rod (45) rotatably arranged on the turntable (6), and the second cleaning motor (44) is used for driving the second cleaning screw rod (45) to rotate; a second sliding seat (46) is slidably arranged on the rotary table (6), the second sliding seat (46) and the second cleaning screw rod (45) form a screw pair, a second connecting pipe (47) is fixedly connected to the second sliding seat (46), one end of a drying cylinder (50) is connected with a second meshing gear (48), and an electric heating wire (49) is arranged inside the drying cylinder (50); a gear ring (43) is rotatably arranged on the inner side of the top cylinder (5), a motor I (3) is arranged on the outer side of the top cylinder (5), a gear I (4) forming a gear pair with the gear ring (43) is connected to an output shaft of the motor I (3), and when the meshing gear I (41) and the meshing gear II (48) are respectively meshed with the gear ring (43), the cleaning cylinder (42) and the drying cylinder (50) are driven to rotate through the gear ring (43).
9. The apparatus for analyzing and detecting a specific protein with an automatic sample addition function according to claim 1, wherein: the injection assembly comprises a position electric cylinder (54) arranged on a rotary table (6), a connecting rod (55) is connected to a telescopic rod of the position electric cylinder (54), one end of the connecting rod (55) is fixedly connected with an injection tube (56), a quantitative air cylinder (52) is arranged on the rotary table (6), a piston plate is connected to the telescopic rod of the quantitative air cylinder (52), and the piston plate is in sliding connection with the inner side of the quantitative cylinder (53).
10. The apparatus for analyzing and detecting a specific protein with an automatic sample addition function according to claim 1, wherein: the reagent cylinder (7) and the solution cylinder (8) are respectively communicated with the cleaning cavity through a first pipeline (15), and the valve seat (39) is respectively communicated with the cleaning cavity and the collecting cavity through a second pipeline (16); the quantitative barrels (53) on the two injection assemblies are respectively communicated with the reagent barrel (7) and the solution barrel (8) through a pipeline III (51), and the injection tube (56) is communicated with the quantitative barrels (53) through a pipeline IV (57).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310640591.8A CN116660547A (en) | 2023-06-01 | 2023-06-01 | Specific protein analysis and detection device with automatic sample adding function |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310640591.8A CN116660547A (en) | 2023-06-01 | 2023-06-01 | Specific protein analysis and detection device with automatic sample adding function |
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| Publication Number | Publication Date |
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| CN116660547A true CN116660547A (en) | 2023-08-29 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202310640591.8A Pending CN116660547A (en) | 2023-06-01 | 2023-06-01 | Specific protein analysis and detection device with automatic sample adding function |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118578298A (en) * | 2024-08-07 | 2024-09-03 | 兴化市营丰机械厂 | A shot blasting equipment for multifunctional workpieces |
| CN118759208A (en) * | 2024-09-03 | 2024-10-11 | 湖南合创思医疗科技有限公司 | A fully automatic enzyme-linked immunosorbent assay device and method thereof |
| CN118883480A (en) * | 2024-09-29 | 2024-11-01 | 江苏恩莫阿赛生物技术有限公司 | A bovine serum albumin purity detection device |
| CN118914476A (en) * | 2024-10-09 | 2024-11-08 | 内蒙古鄂尔多斯电力冶金集团股份有限公司 | Alkali solution concentration detection device |
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2023
- 2023-06-01 CN CN202310640591.8A patent/CN116660547A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118578298A (en) * | 2024-08-07 | 2024-09-03 | 兴化市营丰机械厂 | A shot blasting equipment for multifunctional workpieces |
| CN118759208A (en) * | 2024-09-03 | 2024-10-11 | 湖南合创思医疗科技有限公司 | A fully automatic enzyme-linked immunosorbent assay device and method thereof |
| CN118883480A (en) * | 2024-09-29 | 2024-11-01 | 江苏恩莫阿赛生物技术有限公司 | A bovine serum albumin purity detection device |
| CN118914476A (en) * | 2024-10-09 | 2024-11-08 | 内蒙古鄂尔多斯电力冶金集团股份有限公司 | Alkali solution concentration detection device |
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