CN116531477A - A Chinese veterinary drug oral preparation for veterinary treatment of liver diseases and its preparation method and application - Google Patents

Abstract

The invention discloses a veterinary Chinese medicine oral preparation for treating liver diseases, and a preparation method and application thereof, and belongs to the technical field of veterinary medicines. The raw materials for preparing the traditional Chinese veterinary medicine oral preparation comprise the following components in parts by weight: 20-30 parts of radix curcumae, 25-35 parts of radix astragali, 1-10 parts of radix isatidis, 5-15 parts of semen cuscutae, 1-3 parts of bighead atractylodes rhizome and 1-2 parts of white paeony root. The veterinary Chinese veterinary drug oral preparation for treating liver diseases takes radix curcumae as a monarch, radix astragali as a minister, radix isatidis and semen cuscutae as an adjuvant, and rhizoma atractylodis macrocephalae and radix paeoniae alba as a guide, and can sooth liver, promote bile flow, nourish liver and kidney, promote blood circulation to remove blood stasis, so that the laying rate of laying hens can be well improved, and the eggshell quality can be improved.

Description

A Chinese veterinary drug oral preparation for veterinary treatment of liver diseases and its preparation method, application

technical field

The invention belongs to the technical field of veterinary medicines, and in particular relates to a traditional Chinese veterinary medicine oral preparation for veterinary treatment of liver diseases, a preparation method and application thereof.

Background technique

With the rapid development and improvement of my country's animal husbandry and the further improvement of people's living and cultural standards, the issue of animal-derived food safety has increasingly become the core of people's close attention. With the implementation of relevant national policies of "reducing antibiotics" and "replacing antibiotics", how to ensure the safety and quality of livestock and poultry animal-derived products and reduce the side effects and residues of antibiotics used in livestock and poultry is an urgent issue for the animal husbandry and breeding industry. problem to be solved. Chinese veterinary drug products can effectively solve the above problems due to their advantages of safety, effectiveness and less side effects, and can make great contributions to the development of livestock and poultry breeding in my country. The advantages of Chinese veterinary medicines in the process of use have gradually been highly valued by Chinese veterinary medicine research and development technicians and livestock and poultry producers and breeders of Chinese veterinary medicines. The industrial output value of traditional Chinese veterinary medicine has increased significantly, and is gradually approaching the industrial goal of 10 billion yuan.

The low laying performance of laying hens is an important factor that seriously endangers the laying hen breeding industry. It can not only lead to low production performance and decline in the quality of poultry products, but also affect people's economic income and foreign trade. In recent years, with the continuous increase in the number of laying hens in my country, there are many factors affecting the production performance of laying hens. For example, long-term use of hormone drugs to regulate the production performance of laying hens will easily lead to drug resistance, residual, toxic and side effects, etc. insufficient.

Contents of the invention

In view of the above problems, the present invention attempts to screen the compound drug combination from the perspectives of soothing the liver and gallbladder, nourishing the liver and kidney, promoting blood circulation and removing blood stasis, so as to promote the nutritional metabolism and biotransformation of the liver of laying hens, so as to achieve the purpose of increasing egg production rate and improving eggshell quality . The oral preparation of Chinese veterinary medicine of the present invention, with curcuma as king, astragalus as minister, Radix Radix and Cuscuta as assistant, Atractylodes macrocephala and Radix Paeoniae Alba as envoy, cooperates to invigorate qi and nourish blood, regulate liver and kidney, promote blood circulation and remove blood stasis, clear away heat and nourish The role of Yin.

The first aspect of the present invention is to disclose a Chinese veterinary drug oral preparation for veterinary treatment of liver diseases. The prepared raw materials include the following components in parts by weight:

20-30 parts of curcuma, 25-35 parts of astragalus, 1-10 parts of isatidis, 5-15 parts of dodder, 1-3 parts of Atractylodes macrocephala, and 1-2 parts of white peony.

In some embodiments of the present invention, in the prepared raw materials, the weight ratio of the turmeric, astragalus, isatis root, dodder, atractylodes rhizome and white peony root is 1: (1-1.5): (0.1-0.4): (0.1- 0.5): (0.05-0.1): (0.01-0.05).

In some embodiments of the present invention, the weight ratio of the curcuma, Radix Astragali, Banlangen, Dodder, Atractylodes Rhizome and Radix Paeoniae Alba is 1: (1.1-1.3): (0.1-0.3): (0.3-0.5): (0.06 -0.1): (0.02-0.05).

In some embodiments of the present invention, the prepared raw materials include the following components in parts by weight:

25 parts of turmeric, 30 parts of astragalus, 5 parts of isatidis, 10 parts of dodder, 2 parts of Atractylodes macrocephala, and 1 part of white peony.

In some embodiments of the present invention, the prepared raw materials also include oral preparation excipients.

In some embodiments of the present invention, the oral preparation excipients include deionized water and preservatives.

In some embodiments of the present invention, the turmeric, astragalus, isatidis, dodder, atractylodes rhizome and white peony root are extracted with polar solvents, such as water, ethanol or their aqueous solutions and other polar solvents.

In some embodiments of the present invention, the polar solvent extracts of turmeric, astragalus, isatidis, dodder, atractylodes rhizome and white peony root are preserved in the raw material form of extraction stock solution, diluent, concentrated solution or dry powder.

The second aspect of the present invention is to disclose the preparation method of the veterinary Chinese veterinary drug oral preparation for treating liver diseases described in the first aspect, comprising the following steps:

S01, taking various Chinese herbal medicine raw materials and pulverizing them to obtain raw materials for extraction;

S02, extracting the extraction raw material, the extracted solvent is ethanol aqueous solution;

S03 is filtered to obtain the extract;

S04, add excipients, prepare, and obtain the veterinary Chinese veterinary drug oral preparation for treating liver diseases.

In some embodiments of the present invention, in S02, the extraction solvent is 8-10 times the volume of 60-80% (v/v) ethanol aqueous solution.

In some embodiments of the present invention, in S02, extract at 50-75°C for 2-5 hours, preferably 2-3 times.

In some embodiments of the present invention, during the process of extracting the extracted raw materials, mechanical agitation is used at the same time to improve the extraction effect and reduce the problem of uneven distribution of local materials. The fuzzy control algorithm is used to process the real-time temperature acquired by the temperature sensor in the stirring vessel to dynamically adjust the stirring speed, the steps are as follows:

(1) Define the input variable reaction temperature (T), the reaction temperature change rate (dT/dt) and the output variable stirring speed (v), read the temperature and temperature change rate, and input them into the fuzzy controller;

(2) Initialize the fuzzy controller, calculate the weight value of each rule and the membership function of the output variable according to the membership function of the input variable and the set rule base, and combine the weight value and membership function of all rules Calculate the output membership function to obtain the output membership function; calculate the center of gravity of the output membership function to obtain the fuzzy output value.

(3) Through the defuzzification conversion, the specific and feasible output stirring speed value v is obtained, and the obtained stirring speed signal is output to the stirrer controller to realize temperature control.

(4) Back to (1), continue to wait for the next measured value of reaction temperature f and reaction temperature change rate Δ _f .

Regulated by self-tuning fuzzy control with the following formula:

Among them, β is the adjustment factor of self-calibration fuzzy control, and its value is [0, 1], which is related to f, Δ _f ; L is the step size of fuzzy control, Mε and M1 are the upper and lower limits of the temperature deviation threshold, f(n ) and f(n-1) are the time deviations of n and n-1 respectively, f(ΔT _c , S, α) is the control correction amount, ΔT _c is the deviation between the temperature target temperature and the actual temperature, S is the heating power, α is the mass transfer coefficient.

The third aspect of the present invention is to disclose the application of the veterinary Chinese veterinary drug oral preparation for treating liver diseases described in the first aspect in improving laying hen egg production rate and eggshell quality.

Beneficial effects of the present invention:

The Chinese veterinary drug oral preparation for veterinary treatment of liver diseases of the present invention uses turmeric as the master, astragalus as the minister, Radix Radix and Cuscuta as assistants, Atractylodes Rhizoma Atractylodes Rhizome and Radix Paeoniae Alba as envoys, soothes the liver and gallbladder, nourishes the liver and kidney, activates blood and removes blood stasis , can very well increase the laying rate of laying hens and improve the quality of eggshells.

Detailed ways

Embodiments of the present invention are described below through specific examples, and those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. The present invention can also be implemented or applied through other different specific implementation modes, and various modifications or changes can be made to the details in this specification based on different viewpoints and applications without departing from the spirit of the present invention.

Unless otherwise specified, examples and comparative examples are parallel tests with the same components, component contents, preparation steps, and preparation parameters.

Example 1

Preparation method of Chinese veterinary drug oral preparation for veterinary treatment of liver disease

(1) Take 20 parts of curcuma, 25 parts of astragalus, 3 parts of Radix Radix, 5 parts of dodder, 1 part of Atractylodes macrocephala, and 1 part of Radix Paeoniae Alba, pulverize and mix to obtain raw materials for extraction;

(2) Add 5 times the volume of 70% ethanol aqueous solution, stir, extract at 65°C for 1 hour, filter, add 5 times the volume of 70% ethanol aqueous solution again, stir, extract at 65°C for 2 hours, filter, and combine the two extractions filtrate;

(3) adding 0.1% (w/v) preservative benzoic acid to obtain the veterinary Chinese veterinary drug oral preparation for treating liver diseases.

Example 2

A preparation method of Chinese veterinary drug oral preparation for veterinary treatment of liver disease, the difference from Example 1 is:

The raw materials of Chinese herbal medicine in preparation are: 30 parts of curcuma, 35 parts of astragalus, 10 parts of isatidis, 15 parts of dodder, 3 parts of Atractylodes macrocephala and 2 parts of white peony.

Example 3

A preparation method of Chinese veterinary drug oral preparation for veterinary treatment of liver disease, the difference from Example 1 is:

The raw materials of Chinese herbal medicine in preparation are: 25 parts of curcuma, 30 parts of astragalus, 5 parts of isatidis, 10 parts of dodder, 2 parts of Atractylodes macrocephala and 1 part of white peony.

Example 4

A preparation method of an oral Chinese veterinary medicine for treating liver diseases, the difference from Example 1 is that in the process of extracting the extracted raw materials, mechanical agitation is used to improve the extraction effect and reduce the uneven distribution of local materials. average problem. The fuzzy control algorithm is used to process the real-time temperature acquired by the temperature sensor in the stirring vessel to dynamically adjust the stirring speed, the steps are as follows:

(1) Define the input variable reaction temperature (T), the reaction temperature change rate (dT/dt) and the output variable stirring speed (v), read the temperature and temperature change rate, and input them into the fuzzy controller;

(2) Initialize the fuzzy controller, calculate the weight value of each rule and the membership function of the output variable according to the membership function of the input variable and the set rule base, and combine the weight value and membership function of all rules Calculate the output membership function to obtain the output membership function; calculate the center of gravity of the output membership function to obtain the fuzzy output value.

(3) Through the defuzzification conversion, the specific and feasible output stirring speed value v is obtained, and the obtained stirring speed signal is output to the stirrer controller to realize temperature control.

(4) Back to (1), continue to wait for the next measured value of reaction temperature and reaction temperature change rate.

Regulated by self-tuning fuzzy control with the following formula:

Among them, β is the adjustment factor of self-calibration fuzzy control, and its value is [0, 1], which is related to f, Δ _f ; L is the step size of fuzzy control, Mε and M1 are the upper and lower limits of the temperature deviation threshold, f(n ) and f(n-1) are the time deviations of n and n-1 respectively, f(ΔT _c , S, α) is the control correction amount, ΔT _c is the deviation between the temperature target temperature and the actual temperature, S is the heating power, α is the mass transfer coefficient.

As a result, it was found that for turmeric, astragalus, Radix Radix Radix, Cuscutae, Atractylodes macrocephala, and Radix Paeoniae Alba micropowders below 300 mesh, in the three-blade stirring system heated by the interlayer heating rod of the ethanol aqueous solution extraction solvent of 8 times the volume, the bottom of the reactor was tightly Set at least 3 temperature sensors in a ring to obtain temperature and temperature change rate. The temperature difference between the 2 temperature sensors is greater than 0.2°C or the temperature rise rate is greater than 5°C/min, and the increase rate of the stirrer is 5%. The abnormal deepening of the color of the extract and the generation of by-products can be significantly avoided.

Comparative example 1

A preparation method of Chinese veterinary drug oral preparation for veterinary treatment of liver disease, the difference from Example 1 is:

The raw materials of Chinese herbal medicine in the preparation are: 35 parts of curcuma, 10 parts of astragalus, 3 parts of Radix Radix, 5 parts of dodder, 1 part of Atractylodes macrocephala and 1 part of white peony.

Comparative example 2

A preparation method of Chinese veterinary drug oral preparation for veterinary treatment of liver disease, the difference from Example 1 is:

The raw materials of Chinese herbal medicine in preparation are: 20 parts of curcuma, 25 parts of astragalus, 6 parts of isatidis, 1 part of dodder, 1 part of Atractylodes macrocephala and 1 part of white peony.

Comparative example 3

A preparation method of Chinese veterinary drug oral preparation for veterinary treatment of liver disease, the difference from Example 1 is:

The raw materials of Chinese herbal medicine in the preparation are: 20 parts of turmeric, 25 parts of astragalus, 3 parts of Radix Radix, 5 parts of Cuscuta, and 2 parts of Atractylodes macrocephala.

Effects of Experimental Examples on Egg Production of Laying Hens

Test object: 48-week-old Jingbai No. 1 laying hens

Feeding and management: Layers have free access to food and water, and the chicken house is normally ventilated and supplemented with light. The control group was fed with basal rations, and the basal rations of the test group were supplemented with Chinese veterinary drug oral preparations equivalent to 0.5 g/kg crude drug of the examples and comparative examples (can be appropriately concentrated in volume).

There were no less than 50 laying hens in each test group and control group.

Count the egg production and eggshell quality of laying hens after four weeks, and the results are shown in Table 1 and Table 2. Egg production rate = number of eggs laid per day/total number of laying hens × 100%, average egg weight = total egg weight/total number of eggs. Eggshell thickness Use a vernier caliper to measure the thickness of the tip, blunt end, and side end of the eggshell (remove the mucous membrane) of the egg, and calculate the average value. Eggshell strength was tested using an eggshell strength tester (eggshell strength tester ORKA).

Table 1 egg production performance

The study found that the egg production rate and average egg weight of Examples 1-3 were significantly better than those of Comparative Example 1, and Comparative Example 1 was significantly better than Comparative Examples 2, 3 and the blank control group. The results show that the composition and content of Chinese herbal medicine obtained in the oral preparation of Chinese veterinary medicine of the present invention significantly affect the laying performance of laying hens.

Table 2 eggshell quality

Egg shell thickness mm Egg shell strength kg/m ² Example 1 0.34±0.02b 4.54±0.18b Example 2 0.34±0.01b 4.48±0.27b Example 3 0.34±0.01b 4.49±0.30b Comparative example 1 0.32±0.02a 4.31±0.19a Comparative example 2 0.32±0.01a 4.38±0.64a Comparative example 3 0.32±0.01a 4.32±0.30a control group (blank) 0.32±0.01a 4.37±0.58a

The research found that the eggshell thickness and eggshell strength of Examples 1-3 were significantly better than those of Comparative Examples 1-3 and the blank control group. The results show that the Chinese herbal medicine components and contents obtained in the oral preparation of the Chinese veterinary medicine of the present invention significantly affect the eggshell quality of laying hens.

The preferred specific implementations and examples of the present invention have been described in detail above, but the present invention is not limited to the above-mentioned implementations and examples. Within the scope of knowledge of those skilled in the art, it is also possible to Various changes are made.

Claims (10)

1. The veterinary drug oral preparation for treating liver diseases is characterized in that the prepared raw materials comprise the following components in parts by weight:

20-30 parts of radix curcumae, 25-35 parts of radix astragali, 1-10 parts of radix isatidis, 5-15 parts of semen cuscutae, 1-3 parts of bighead atractylodes rhizome and 1-2 parts of white paeony root.

2. The veterinary drug oral preparation for treating liver diseases according to claim 1, wherein the weight ratio of the radix curcumae, the radix astragali, the radix isatidis, the semen cuscutae, the rhizoma atractylodis macrocephalae and the radix paeoniae alba is 1: (1-1.5): (0.1-0.4): (0.1-0.5): (0.05-0.1): (0.01-0.05).

3. The veterinary drug oral preparation for treating liver diseases according to claim 1 or 2, wherein the weight ratio of radix curcumae, radix astragali, radix isatidis, semen cuscutae, rhizoma atractylodis macrocephalae and radix paeoniae alba is 1: (1.1-1.3): (0.1-0.3): (0.3-0.5): (0.06-0.1): (0.02-0.05).

4. A veterinary drug oral formulation for treating liver diseases according to any one of claims 1-3, wherein the raw materials prepared comprise the following components in parts by weight:

25 parts of radix curcumae, 30 parts of astragalus, 5 parts of radix isatidis, 10 parts of semen cuscutae, 2 parts of bighead atractylodes rhizome and 1 part of white paeony root.

5. The veterinary drug oral preparation for treating liver diseases according to any one of claims 1 to 4, wherein the prepared raw materials further comprise oral preparation auxiliary materials.

6. The veterinary drug oral formulation for treating liver diseases according to any one of claims 1 to 5, wherein the oral formulation auxiliary materials comprise deionized water and a preservative.

7. A method for preparing an oral formulation of a veterinary drug for the treatment of liver diseases according to any one of claims 1 to 6, comprising the steps of:

s01, taking all the Chinese herbal medicine raw materials, and crushing to obtain an extraction raw material;

s02, extracting the extraction raw materials, wherein an extraction solvent is ethanol water solution;

s03, filtering to obtain an extracting solution;

s04, adding auxiliary materials, and blending to obtain the veterinary drug oral preparation for treating liver diseases.

8. The method for preparing an oral preparation of veterinary drugs for treating liver diseases according to claim 7, wherein in S02, the extracted solvent is an aqueous solution of 60-80% (v/v) ethanol in an amount of 8-10 times the volume.

9. The method of preparing an oral formulation of veterinary medicine for treating liver diseases according to claim 7 or 8, wherein in S02, the extraction is performed for 2-5 hours, preferably 2-3 times, at 50-75 ℃.

10. Use of a veterinary drug oral formulation according to any one of claims 1-6 for the treatment of liver diseases for increasing the laying rate of laying hens and for increasing the eggshell quality.