CN116515621B - Multi-temperature-zone PCR amplification system, method and equipment - Google Patents
Multi-temperature-zone PCR amplification system, method and equipment Download PDFInfo
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- CN116515621B CN116515621B CN202310507650.4A CN202310507650A CN116515621B CN 116515621 B CN116515621 B CN 116515621B CN 202310507650 A CN202310507650 A CN 202310507650A CN 116515621 B CN116515621 B CN 116515621B
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- 238000012408 PCR amplification Methods 0.000 title claims abstract description 54
- 238000000034 method Methods 0.000 title claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 104
- 230000007246 mechanism Effects 0.000 claims abstract description 78
- 230000001105 regulatory effect Effects 0.000 claims description 26
- 239000007788 liquid Substances 0.000 claims description 22
- 230000001276 controlling effect Effects 0.000 claims description 19
- 239000002699 waste material Substances 0.000 claims description 15
- 239000003153 chemical reaction reagent Substances 0.000 claims description 7
- 238000007789 sealing Methods 0.000 claims description 5
- 230000006978 adaptation Effects 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 abstract description 4
- 238000007689 inspection Methods 0.000 description 7
- 238000003752 polymerase chain reaction Methods 0.000 description 6
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 238000004891 communication Methods 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 238000004804 winding Methods 0.000 description 2
- 230000004543 DNA replication Effects 0.000 description 1
- 239000012807 PCR reagent Substances 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 150000002972 pentoses Chemical class 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
- B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1838—Means for temperature control using fluid heat transfer medium
- B01L2300/185—Means for temperature control using fluid heat transfer medium using a liquid as fluid
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The utility model provides a multi-temperature-zone PCR amplification system, a multi-temperature-zone PCR amplification method and multi-temperature-zone PCR amplification equipment. According to the utility model, by arranging the water bath constant temperature mechanism and the test tube steering adjusting mechanism, people can drive the test tube to transfer among the first constant temperature water bath, the second constant temperature water bath and the third constant temperature water bath by operating the test tube steering adjusting mechanism, so that a PCR test is performed, traditional air pressure control is replaced, external air control equipment is not needed, the test tube is convenient for people to carry, and the test tube steering adjusting mechanism is suitable for on-site POTC instant test, and the water bath constant temperature mechanism and the test tube steering adjusting mechanism can be matched for centrifugation of a test tube tester, so that the test tube steering adjusting mechanism has strong functionality.
Description
Technical Field
The present utility model relates toInspection apparatusIn particular, to a multi-temperature zone PCR amplification system, method and apparatus.
Background
The Polymerase Chain Reaction (PCR) is a molecular biological technology for amplifying specific DNA fragments, and can be regarded as special DNA replication outside organisms, and the greatest characteristic of the PCR is that trace DNA can be greatly increased, the PCR utilizes the fact that DNA becomes single-stranded at a high temperature of 95 ℃ in vitro, a primer and the single-stranded are combined according to the base complementary pairing principle at a low temperature (usually about 60 ℃), the temperature is adjusted to the optimal reaction temperature (about 72 ℃) of DNA polymerase, and the DNA polymerase synthesizes the complementary strand along the direction from phosphoric acid to pentose (5 '-3').
At present, most PCR amplification devices are mainly controlled by air pressure to continuously move PCR reagents back and forth in three different temperature areas to realize control of the temperature rising and reducing process, and the scheme needs to be externally connected with an air pressure control device, so that the whole volume of the PCR amplification device is larger, the PCR amplification device is inconvenient to carry, and the PCR amplification device is not suitable for on-site POTC instant inspection.
For example: the specification of the portable PCR amplification instrument disclosed in Chinese patent/utility model (utility model number: CN 202023192020.3) discloses that: the PCR amplification instrument in the prior art has the problem of inconvenient carrying and moving; the above patent can be used to demonstrate the drawbacks of the prior art.
Therefore, we have made improvements to this and proposed multi-temperature zone PCR amplification systems, methods and apparatus.
Disclosure of Invention
The utility model aims at: aiming at the fact that the existing PCR amplification device is large in whole size, inconvenient to carry and not suitable for on-site POTC instant test.
In order to achieve the above object, the present utility model provides the following multi-temperature zone PCR amplification system, method and apparatus, to improve the above problems.
The utility model is specifically as follows:
the multi-temperature-zone PCR amplification system comprises PCR amplification equipment, wherein a constant temperature module for heating a test tube and an adjusting module for adjusting the position of the test tube are arranged on the PCR amplification equipment;
the constant temperature module is specifically a water bath constant temperature mechanism, and the water bath constant temperature mechanism comprises a first constant temperature water bath, a second constant temperature water bath and a third constant temperature water bath;
the adjusting module is specifically a test tube steering adjusting mechanism, and through setting up water bath constant temperature mechanism and test tube steering adjusting mechanism, make people turn to adjusting mechanism through operating the test tube, can drive the test tube and shift between first constant temperature water bath, second constant temperature water bath and third constant temperature water bath, carry out the PCR test, traditional atmospheric pressure control has been replaced, do not need external pneumatic control equipment, the people of being convenient for carry, the instant inspection of POTC of applicable scene, and water bath constant temperature mechanism and test tube steering adjusting mechanism cooperation can also be used for the centrifugation of test tube test machine, and is functional strong.
As the preferable technical scheme of the utility model, the PCR amplification equipment further comprises a waste liquid mechanism for collecting waste liquid, by arranging the waste liquid mechanism, the inspected test tube can be placed in the test tube groove on the second round wheel, when one of the reels rotates to roll the sterilized paper towel, the sterilized paper towel can drive the first round wheel to rotate, the second round wheel can rotate, the liquid in the test tube is poured onto the sterilized paper towel and finally rolled on one of the reels, and the first round wheel and the second round wheel can be matched with the concave frame to clamp different pipetting guns, so that the PCR amplification equipment is high in functionality.
A multi-temperature zone PCR amplification method comprising the steps of:
firstly, placing a reagent, and placing a test tube filled with the reagent and to-be-tested liquid on a test tube steering adjusting mechanism;
step two, centrifuging, controlling a test tube steering regulating mechanism and a water bath constant temperature mechanism to rotate the test tube at a high speed, and controlling the water bath constant temperature mechanism and the test tube steering regulating mechanism to stop rotating the test tube after centrifuging;
step three, checking, namely controlling the test tube steering regulating mechanism to enable test tubes on the test tube steering regulating mechanism to be respectively soaked in a first constant temperature water bath, a second constant temperature water bath and a third constant temperature water bath with different temperatures, switching by controlling the test tube steering regulating mechanism, and controlling the test tube steering regulating mechanism to lift after the checking is finished, and taking out the test tubes.
The utility model provides a multi-temperature area PCR amplification equipment, includes and bears seat and sets up the lid that bears seat one side, first constant temperature water bath, second constant temperature water bath and third constant temperature water bath evenly set up in the top that bears the seat, water bath constant temperature mechanism still includes three first spring telescopic link that sets up respectively in first constant temperature water bath, second constant temperature water bath and third constant temperature water bath inner chamber bottom, threaded connection has the paddle on the first spring telescopic link, the screw thread of looks adaptation is all offered along the outer middle part with the paddle of first spring telescopic link, through setting up first spring telescopic link and paddle, makes the chassis when getting into first constant temperature water bath, second constant temperature water bath or third constant temperature water bath, can promote the paddle decline to rotate, and then to the water stirring in first constant temperature water bath, second constant temperature water bath or the third constant temperature water bath, the temperature inspection of messenger's first constant temperature water bath, second constant temperature bath or third constant temperature water bath is more even, and can drive the temperature test tube through setting up first constant temperature telescopic link and paddle, and can drive the temperature test tube in the second constant temperature bath, the rotation position, and can drive the temperature tube in the second constant temperature tube.
As a preferable technical scheme of the utility model, arc grooves are formed in one sides of the first constant temperature water bath pool, the second constant temperature water bath pool and the third constant temperature water bath pool, which are close to the center of the bearing seat, a first spring is fixedly connected to the bottom end inside the arc grooves, and an arc sealing plate is fixedly connected to the top end of the first spring.
As the preferable technical scheme of the utility model, the test tube steering adjusting mechanism is arranged in the middle of the top end of the bearing seat, the test tube steering adjusting mechanism comprises a first electric push rod fixedly arranged at the bottom end of the inner cavity of the bearing seat, the top end of the first electric push rod is connected with an adjusting block through a bearing, three circular arc grooves matched with paddles are formed in the adjusting block, a first T-shaped block is fixedly connected in one circular arc groove, an annular frame is arranged on the first T-shaped block, a first T-shaped groove for the first T-shaped block to slide is formed in the circumferential side of the annular frame, and by arranging the test tube steering adjusting mechanism, people can drive a disc to rise when the adjusting block rises by operating the first electric push rod, and then drive the test tube to move into different first constant temperature water baths, second constant temperature water baths or third constant temperature water baths under the action of a toothed ring and paddles.
As the preferable technical scheme of the utility model, a disc for placing test tubes is arranged in the annular frame, a bottom frame is arranged at the bottom of the disc, a plurality of connecting rods are arranged between the disc and the bottom frame, and the middle part of the bottom end of the bottom frame is provided with the bottom frame for the first spring telescopic rod to pass through.
As the preferable technical scheme of the utility model, the top end of the bearing seat is provided with a toothed ring for driving the blades and the annular frame to rotate through a bearing, the outer edges of the blades, the underframe and the annular frame are provided with teeth matched with the toothed ring, the top end of the bearing seat is symmetrically provided with two motors, the output shaft of each motor is fixedly connected with a gear for driving the toothed ring to rotate, and the bottom of the inner side of each circular arc groove is provided with teeth matched with the blades and the annular frame.
As the preferable technical scheme of the utility model, the waste liquid mechanism comprises an arc-shaped frame arranged at the front side and the rear side of a bearing seat, two first round wheels are arranged in the arc-shaped frame, two opposite sides of the first round wheels are fixedly connected with second round wheels, semicircular grooves for clamping test tubes are formed in the second round wheels, a round shaft is arranged between the arc-shaped frame and the first round wheels, a plurality of elastic sheets distributed along the circumference and transversely are fixedly connected onto the round shaft, clamping blocks are fixedly connected onto the elastic sheets, a plurality of grooves for inserting the clamping blocks are fixedly connected onto the middle of the second round wheels, reels are arranged at two sides of the arc-shaped frame, one end of each reel is connected with one side of the bearing seat through a bearing, disinfection paper towels penetrating through the first round wheels and the arc-shaped frame are wound on the reels, and through the clamping blocks and the grooves, people can adjust the distance between the two second round wheels according to the first round wheels at the outer sides of different pulling sides of the test tubes, and further different test tubes can be placed between the two second round wheels in a proper mode.
As the preferable technical scheme of the utility model, the waste liquid mechanism further comprises two concave frames which are respectively arranged on the front side and the rear side of the bearing seat, one end of each concave frame is connected with one side of the box cover through a rotating shaft, the other ends of the concave frames are connected with a second T-shaped block through rotating shafts, one ends of the front side and the rear side of the bearing seat are respectively provided with a second T-shaped groove for inserting the second T-shaped block, a second spring is arranged in each second T-shaped groove, people can upwards lift the box cover through the concave frames and the second T-shaped blocks, the box cover can move to one side of the bearing seat, and when the box cover covers the bearing seat, the concave frames can be matched with the first round wheels and the arc frames to clamp the pipetting gun.
Drawings
FIG. 1Provided for the utility modelMulti-temperature-zone PCR amplification systemSchematic of (2);
FIG. 2 shows the present utility modelMulti-temperature-zone PCR amplification equipmentIs a structural schematic diagram of (a);
FIG. 3 shows the present utility modelMulti-temperature-zone PCR amplification equipmentIs a schematic cross-sectional structure of the bearing seat;
FIG. 4 shows the present utility modelMulti-temperature-zone PCR amplification equipmentAn enlarged schematic view of the structure at a in fig. 3;
FIG. 5 shows the present utility modelMulti-temperature-zone PCR amplification equipmentIs a schematic cross-sectional structure of the annular frame;
FIG. 6 shows the present utility modelMulti-temperature-zone PCR amplification equipmentIs a schematic cross-sectional structure of the arc-shaped frame;
FIG. 7 shows the present utility modelMulti-temperature-zone PCR amplification equipmentAn enlarged schematic view of the structure at B in fig. 6;
FIG. 8 shows the present utility modelMulti-temperature-zone PCR amplification equipmentA cross-sectional structure schematic diagram of the joint of the bearing seat and the box cover;
FIG. 9 shows the present utility modelMulti-temperature-zone PCR amplification equipmentIs a schematic cross-sectional view of the carrier gear.
The figures indicate:
1. a bearing seat;
2. a water bath constant temperature mechanism; 201. a first constant temperature water bath; 202. a second constant temperature water bath; 203. a third constant temperature water bath; 204. a first spring telescoping rod; 205. a paddle; 206. an arc-shaped groove; 207. a first spring; 208. arc-shaped sealing plates;
3. a test tube steering adjustment mechanism; 301. a first electric push rod; 302. an adjusting block; 303. a motor; 304. a gear; 305. a toothed ring; 306. an annular frame; 307. a first T-shaped slot; 308. a first T-block; 309. a connecting rod; 310. a chassis; 311. a disc;
4. a waste liquid mechanism; 401. an arc-shaped frame; 402. a first round wheel; 403. a second round wheel; 404. a circular shaft; 405. a groove; 406. a spring plate; 407. a clamping block; 408. a reel; 409. a concave frame; 410. a second T-shaped slot; 411. a second spring; 412. a second T-block;
5. and a box cover.
Detailed Description
In order that those skilled in the art will better understand the present utility model, a technical solution in the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in which it is apparent that the described embodiments are only some embodiments of the present utility model, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present utility model without making any inventive effort, shall fall within the scope of the present utility model.
As described in the background art, the whole PCR amplification device is large in size, inconvenient to carry and not suitable for on-site POTC instant test.
In order to solve the technical problem, the utility model provides a multi-temperature-zone PCR amplification system, a multi-temperature-zone PCR amplification method and multi-temperature-zone PCR amplification equipment, which are applied to reduce the volume of a PCR amplification device, are convenient to carry and are suitable for on-site POTC instant inspection.
Specifically, please refer to the drawings1The multi-temperature-zone PCR amplification system specifically comprises:
the PCR amplification equipment is provided with a constant temperature module for heating the test tube and an adjusting module for adjusting the position of the test tube;
the constant temperature module is specifically a water bath constant temperature mechanism 2, and the water bath constant temperature mechanism 2 comprises a first constant temperature water bath 201, a second constant temperature water bath 202 and a third constant temperature water bath 203;
the regulating module is specifically a test tube steering regulating mechanism 3, through setting up water bath constant temperature mechanism 2 and test tube steering regulating mechanism 3, make people turn to regulating mechanism 3 through operating the test tube, alright drive the test tube and shift between first constant temperature water bath 201, second constant temperature water bath 202 and third constant temperature water bath 203, carry out the PCR test, traditional atmospheric pressure control has been replaced, do not need external pneumatic control equipment, people carry conveniently, the instant inspection of POTC of applicable scene, and water bath constant temperature mechanism 2 and test tube steering regulating mechanism 3 cooperation can also be used for the centrifugation of test tube test machine, and is functional strong.
The PCR amplification device further comprises a waste liquid mechanism 4 for collecting waste liquid, the waste liquid mechanism 4 is arranged, the test tube after inspection can be placed in a test tube groove on the second round wheel 403, when one of the reels 408 is rotated by people to roll up the sterilized paper towel, the sterilized paper towel can drive the first round wheel 402 to rotate, the second round wheel 403 is further rotated, liquid in the test tube is poured onto the sterilized paper towel and finally rolled up on one of the reels 408, and the first round wheel 402 and the second round wheel 403 can be matched with the concave frame 409 to clamp different pipette guns, so that the PCR amplification device is high in functionality.
In order to make the person skilled in the art better understand the solution of the present utility model, the technical solution of the embodiment of the present utility model will be clearly and completely described below with reference to the accompanying drawings.
It should be noted that, under the condition of no conflict, the embodiments of the present utility model and the features and technical solutions in the embodiments may be combined with each other.
It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further definition or explanation thereof is necessary in the following figures.
Example 1
Referring to FIG. 1, the multi-temperature region PCR amplification method comprises the following steps:
firstly, placing a reagent, and placing a test tube filled with the reagent and to-be-tested liquid on a test tube steering adjusting mechanism 3;
step two, centrifuging, controlling the test tube steering regulating mechanism 3 and the water bath constant temperature mechanism 2 to rotate the test tube at a high speed, and controlling the water bath constant temperature mechanism 2 and the test tube steering regulating mechanism 3 to stop rotating the test tube after centrifuging;
step three, checking, namely controlling the test tube steering regulating mechanism 3 to enable test tubes on the test tube steering regulating mechanism 3 to be respectively soaked in a first constant temperature water bath 201, a second constant temperature water bath 202 and a third constant temperature water bath 203 with different temperatures, switching by controlling the test tube steering regulating mechanism 3, and controlling the test tube steering regulating mechanism 3 to lift after the checking is finished, and taking out the test tubes.
Example 2
The multi-temperature-area PCR amplification device specifically comprises a bearing seat 1 and a box cover 5 arranged on one side of the bearing seat 1, wherein the first and second constant temperature water baths 201, 202 and 203 are uniformly arranged at the top end of the bearing seat 1, the water bath constant temperature mechanism 2 further comprises three first spring telescopic rods 204 respectively arranged at the bottom ends of the inner cavities of the first and second constant temperature water baths 201, 202 and 203, the first spring telescopic rods 204 are connected with paddles 205 in a threaded manner, the outer edges of the first spring telescopic rods 204 are provided with threads matched with the middle parts of the paddles 205, and the paddles 205 and the first spring telescopic rods 204 are arranged to enable the bottom frame 310 to push the paddles 205 to descend and rotate when entering the first and second constant temperature water baths 201, 202 and 203, so that the first and second constant temperature water baths 202 and 203 are subjected to water stirring in the first and second constant temperature water baths 202 or 203, and the third constant temperature baths 202 can be further enabled to enter the annular constant temperature adjusting frame 202 or 203 in a rotating mode through the paddles 302 or a rotating mode, so that the temperature of the paddles 203 can be more uniformly adjusted, and the temperature of the paddles 203 can be adjusted by rotating the temperature adjusting frame is enabled to be more uniform, and the temperature of the paddles 203 can be adjusted by the rotating temperature of the temperature adjusting frame or the temperature of the first constant temperature bath 200.
Further, as shown in fig. 2, 3 and 4, the first, second and third constant temperature water baths 201, 202 and 203 are provided with arc grooves 206 on the sides close to the center of the bearing seat 1, the bottom inside the arc grooves 206 is fixedly connected with a first spring 207, the top end of the first spring 207 is fixedly connected with an arc sealing plate 208, and when the adjusting block 302 is lifted and lowered by setting the arc sealing plate 208 and the first spring 207, water in the first, second or third constant temperature water baths 201, 202 or 203 cannot flow out.
Example 3
As shown in fig. 2, 3, 5 and 9, the tube steering adjusting mechanism 3 is disposed in the middle of the top end of the bearing seat 1, the tube steering adjusting mechanism 3 includes a first electric push rod 301 fixedly installed at the bottom end of the inner cavity of the bearing seat 1, the top end of the first electric push rod 301 is connected with an adjusting block 302 through a bearing, three circular arc grooves adapted to the paddle 205 are formed in the adjusting block 302, a first T-shaped block 308 is fixedly connected to the inside of one circular arc groove, an annular frame 306 is disposed on the first T-shaped block 308, a first T-shaped groove 307 for sliding the first T-shaped block 308 is formed in the circumferential side of the annular frame 306, and by disposing the tube steering adjusting mechanism 3, people can drive the disc 311 to rise when the adjusting block 302 rises by operating the first electric push rod 301, and then drive the tube to move to different first constant temperature water baths 201, second constant temperature water baths 202 or third constant temperature water baths 203 under the action of the toothed ring 305 and the paddle 205.
Further, as shown in fig. 2, 3, 5 and 9, a disc 311 for placing test tubes is arranged inside the ring frame 306, a bottom frame 310 is arranged at the bottom of the disc 311, a plurality of connecting rods 309 are arranged between the disc 311 and the bottom frame 310, a bottom frame 310 through which the first spring telescopic rod 204 passes is arranged in the middle of the bottom end of the bottom frame 310, and the first spring telescopic rod 204 does not influence the descent of the disc 311 when the disc 311 descends through the bottom frame 310.
Further, as shown in fig. 2, 3, 5 and 9, the top end of the bearing seat 1 is provided with a toothed ring 305 for driving the blade 205 and the annular frame 306 to rotate through a bearing, teeth matched with the toothed ring 305 are arranged on the outer edges of the blade 205, the bottom frame 310 and the annular frame 306, two motors 303 are symmetrically installed on the top end of the bearing seat 1, a gear 304 for driving the toothed ring 305 to rotate is fixedly connected to an output shaft of each motor 303, teeth matched with the blade 205 and the annular frame 306 are arranged at the bottom of the inner side of each circular arc groove, and the toothed ring 305 can be driven to rotate through the arrangement of the motors 303 and the gear 304, so that the adjusting block 302 is driven to rotate.
Example 4
For further optimization of the multi-temperature-zone PCR amplification device provided in embodiment 2 or 3, specifically, as shown in fig. 2, fig. 6, fig. 7 and fig. 8, the waste liquid mechanism 4 includes an arc-shaped frame 401 disposed on the front and rear sides of the bearing seat 1, two first round wheels 402 are disposed in the arc-shaped frame 401, two second round wheels 403 are fixedly connected to opposite sides of the two first round wheels 402, semicircular grooves for clamping test tubes are disposed on the second round wheels 403, a round shaft 404 is disposed between the arc-shaped frame 401 and the first round wheels 402, a plurality of elastic pieces 406 distributed along the circumference and transversely are fixedly connected to the round shaft 404, a plurality of grooves 405 for inserting the clamping pieces 407 are fixedly connected to the middle of the second round wheels 403, reels 408 are disposed on two sides of the arc-shaped frame 401, one end of each reel 408 is connected to one side of the bearing seat 1 through a bearing, paper towel passing between the first round wheels 402 and the arc-shaped frame 401 is wound on the two reels 408, the two reels can be placed between the two second round wheels 402 according to different circles of the first round wheels 403, and the two different test tubes 403 can be pulled by people, and the two different spaces between the two round wheels can be adjusted by setting up the clamping pieces 407 and the two paper towel wheels can be adjusted.
Further, as shown in fig. 2, 6, 7 and 8, the waste liquid mechanism 4 further includes two concave frames 409 respectively disposed on front and rear sides of the carrying seat 1, one end of each concave frame 409 is connected to one side of the box cover 5 through a rotating shaft, the other end of each concave frame 409 is connected to a second T-shaped block 412 through a rotating shaft, one end of the front and rear sides of the carrying seat 1 is provided with a second T-shaped groove 410 into which the second T-shaped block 412 is inserted, a second spring 411 is disposed inside the second T-shaped groove 410, by disposing the concave frames 409 and the second T-shaped block 412, after the box cover 5 is lifted upwards by people, the box cover 5 can be moved to one side of the carrying seat 1, and when the box cover 5 is covered on the carrying seat 1, the concave frames 409 can be matched with the first round wheel 402 and the arc-shaped frame 401 to clamp the pipette.
The multi-temperature-zone PCR amplification equipment provided by the utility model has the following use process:
taking down a pipette between a concave frame 409 and a first round wheel 402, lifting up a box cover 5, moving the box cover 5 to one side of a bearing seat 1, putting a plurality of test tubes filled with reagents and liquid to be tested on a disc 311, lifting up an adjusting block 302 by controlling a first electric push rod 301, enabling a bottom frame 310 to be in contact with a toothed ring 305, enabling teeth on the adjusting block 302 not to be in contact with paddles 205, controlling a gear 304 to drive the toothed ring 305 to rotate, enabling the bottom frame 310 and the annular frame 306 to rotate at a high speed for centrifugal treatment, controlling the adjusting block 302 to descend after centrifugation, enabling the test tubes to enter a first constant temperature water bath 201, a second constant temperature water bath 202 or a third constant temperature water bath 203, controlling the adjusting block 302 to ascend when the water bath needs to be replaced, the teeth on the regulating block 302 are contacted with the blades 205, the toothed ring 305 is controlled to rotate, the toothed ring 305 drives the regulating block 302 to rotate through the blades 205, then the annular frame 306 and the circular disc 311 rotate to the upper part of the first constant temperature water bath 201, the second constant temperature water bath 202 or the third constant temperature water bath 203, the regulating block 302 is controlled to descend, the test tube enters the first constant temperature water bath 201, the second constant temperature water bath 202 or the third constant temperature water bath 203, after the test is finished, the test tube is placed into the semicircular groove, the winding drum 408 far away from the concave frame 409 is rotated, the sterilizing paper towel drives the first circular wheel 402 to rotate, and then liquid in the test tube drops on the sterilizing paper towel along with the rotation and finally is wound on the winding drum 408.
In the present utility model, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; may be mechanically connected, may be electrically connected or may be in communication with each other; either directly or indirectly, through intermediaries, or both, may be in communication with each other or in interaction with each other, unless expressly defined otherwise. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art according to the specific circumstances.
It is apparent that the above-described embodiments are only some embodiments of the present utility model, but not all embodiments, and the preferred embodiments of the present utility model are shown in the drawings, which do not limit the scope of the patent claims. This utility model may be embodied in many different forms, but rather, embodiments are provided in order to provide a thorough and complete understanding of the present disclosure. Although the utility model has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described in the foregoing description, or equivalents may be substituted for elements thereof. All equivalent structures made by the content of the specification and the drawings of the utility model are directly or indirectly applied to other related technical fields, and are also within the scope of the utility model.
Claims (5)
1. The multi-temperature-zone PCR amplification system is characterized by comprising PCR amplification equipment, wherein a constant temperature module for heating a test tube and an adjusting module for adjusting the position of the test tube are arranged on the PCR amplification equipment;
the constant temperature module is specifically a water bath constant temperature mechanism (2), and the water bath constant temperature mechanism (2) comprises a first constant temperature water bath (201), a second constant temperature water bath (202) and a third constant temperature water bath (203);
the adjusting module is specifically a test tube steering adjusting mechanism (3);
the PCR amplification device comprises a bearing seat (1) and a box cover (5) arranged on one side of the bearing seat (1), wherein a first constant temperature water bath (201), a second constant temperature water bath (202) and a third constant temperature water bath (203) are uniformly arranged at the top end of the bearing seat (1), the water bath constant temperature mechanism (2) further comprises three first spring telescopic rods (204) which are respectively arranged at the bottom ends of inner cavities of the first constant temperature water bath (201), the second constant temperature water bath (202) and the third constant temperature water bath (203), paddles (205) are connected to the first spring telescopic rods (204) in a threaded mode, and matched threads are respectively formed in the outer edges of the first spring telescopic rods (204) and the middle parts of the paddles (205);
an arc-shaped groove (206) is formed in one side, close to the center of the bearing seat (1), of the first constant-temperature water bath (201), the second constant-temperature water bath (202) and the third constant-temperature water bath (203), a first spring (207) is fixedly connected to the bottom end inside the arc-shaped groove (206), and an arc-shaped sealing plate (208) is fixedly connected to the top end of the first spring (207);
the test tube steering adjusting mechanism (3) is arranged in the middle of the top end of the bearing seat (1), the test tube steering adjusting mechanism (3) comprises a first electric push rod (301) fixedly arranged at the bottom end of the inner cavity of the bearing seat (1), the top end of the first electric push rod (301) is connected with an adjusting block (302) through a bearing, three arc grooves matched with the paddles (205) are formed in the adjusting block (302), a first T-shaped block (308) is fixedly connected in one of the arc grooves, an annular frame (306) is arranged on the first T-shaped block (308), and a first T-shaped groove (307) for the first T-shaped block (308) to slide is formed in the circumferential side of the annular frame (306);
the novel test tube rack is characterized in that a disc (311) for placing test tubes is arranged inside the annular frame (306), a bottom frame (310) is arranged at the bottom of the disc (311), a plurality of connecting rods (309) are arranged between the disc (311) and the bottom frame (310), and the middle part of the bottom end of the bottom frame (310) is provided with the bottom frame (310) through which a first spring telescopic rod (204) passes;
the top of bearing seat (1) is provided with through the bearing and is used for driving paddle (205) and annular frame (306) pivoted ring gear (305), paddle (205), chassis (310) and annular frame (306) outer edge be equipped with ring gear (305) looks adaptation tooth, two motors (303) are installed to the top symmetry of bearing seat (1), fixedly connected with is used for driving ring gear (305) pivoted gear (304) on the output shaft of motor (303), the inboard bottom of circular arc groove is equipped with the tooth of paddle (205) and annular frame (306) looks adaptation.
2. The multi-temperature-zone PCR amplification system of claim 1, wherein the PCR amplification apparatus further comprises a waste liquid mechanism (4) for collecting waste liquid.
3. The multi-temperature-zone PCR amplification system according to claim 1, wherein the waste liquid mechanism (4) comprises an arc-shaped frame (401) arranged on the front side and the rear side of the bearing seat (1), two first round wheels (402) are arranged in the arc-shaped frame (401), second round wheels (403) are fixedly connected to opposite sides of the first round wheels (402), semicircular grooves for clamping test tubes are formed in the second round wheels (403), a round shaft (404) is arranged between the arc-shaped frame (401) and the first round wheels (402), a plurality of elastic pieces (406) distributed along the circumference and transversely are fixedly connected to the round shaft (404), clamping blocks (407) are fixedly connected to the elastic pieces (406), grooves (405) for inserting the clamping blocks (407) are formed in the middle of the second round wheels (403), reels (408) are formed in two sides of the arc-shaped frame (401), one end of each reel (408) is connected with one side of the bearing seat (1) through a bearing, and a piece of paper towel (408) penetrates through the reels (408) between the two reels (402).
4. The multi-temperature-zone PCR amplification system according to claim 1, wherein the waste liquid mechanism (4) further comprises two concave frames (409) which are respectively arranged on the front side and the rear side of the bearing seat (1), one end of each concave frame (409) is connected with one side of the box cover (5) through a rotating shaft, the other ends of the concave frames (409) are connected with second T-shaped blocks (412) through rotating shafts, second T-shaped grooves (410) for inserting the second T-shaped blocks (412) are respectively formed in one ends of the front side and the rear side of the bearing seat (1), and second springs (411) are arranged in the second T-shaped grooves (410).
5. A multi-temperature-zone PCR amplification method using the PCR amplification apparatus according to claim 1, comprising the steps of:
firstly, placing a reagent, and placing a test tube filled with the reagent and to-be-tested liquid on a test tube steering adjusting mechanism (3);
step two, centrifuging, namely controlling a test tube steering regulating mechanism (3) and a water bath constant temperature mechanism (2) to rotate the test tube at a high speed, and controlling the water bath constant temperature mechanism (2) and the test tube steering regulating mechanism (3) to stop rotating the test tube after centrifuging;
step three, checking, namely controlling the test tube steering regulating mechanism (3), enabling test tubes on the test tube steering regulating mechanism (3) to be respectively soaked in a first constant temperature water bath (201), a second constant temperature water bath (202) and a third constant temperature water bath (203) with different temperatures, switching through controlling the test tube steering regulating mechanism (3), and controlling the test tube steering regulating mechanism (3) to lift after the checking is finished, and taking out the test tubes.
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| CN202310507650.4A CN116515621B (en) | 2023-05-06 | 2023-05-06 | Multi-temperature-zone PCR amplification system, method and equipment |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202310507650.4A CN116515621B (en) | 2023-05-06 | 2023-05-06 | Multi-temperature-zone PCR amplification system, method and equipment |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103820316A (en) * | 2014-03-09 | 2014-05-28 | 北京工业大学 | Real-time fluorescence PCR (polymerase chain reaction) detection system based on rotary type microfluidic chip |
| CN104130933A (en) * | 2014-08-02 | 2014-11-05 | 张金木 | Thermostatic fluorescence PCR (polymerase chain reaction) amplifier |
| CN109609608A (en) * | 2019-01-17 | 2019-04-12 | 浙江大学 | The linear quick dual temperature PCR amplification automatic control device of one kind and control method |
| CN115477997A (en) * | 2022-10-27 | 2022-12-16 | 北京理工亘舒科技有限公司 | Multi-temperature-zone PCR amplification device and amplification method thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2022022551A (en) * | 2020-06-26 | 2022-02-07 | シスメックス株式会社 | Nucleic acid detection method, nucleic acid detection device and module |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103820316A (en) * | 2014-03-09 | 2014-05-28 | 北京工业大学 | Real-time fluorescence PCR (polymerase chain reaction) detection system based on rotary type microfluidic chip |
| CN104130933A (en) * | 2014-08-02 | 2014-11-05 | 张金木 | Thermostatic fluorescence PCR (polymerase chain reaction) amplifier |
| CN109609608A (en) * | 2019-01-17 | 2019-04-12 | 浙江大学 | The linear quick dual temperature PCR amplification automatic control device of one kind and control method |
| CN115477997A (en) * | 2022-10-27 | 2022-12-16 | 北京理工亘舒科技有限公司 | Multi-temperature-zone PCR amplification device and amplification method thereof |
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