CN116500265B - Pullorum source phage detection device - Google Patents

Pullorum source phage detection device Download PDF

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Publication number
CN116500265B
CN116500265B CN202310749190.6A CN202310749190A CN116500265B CN 116500265 B CN116500265 B CN 116500265B CN 202310749190 A CN202310749190 A CN 202310749190A CN 116500265 B CN116500265 B CN 116500265B
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block
air
mounting
long plate
fixedly arranged
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CN116500265A (en
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郭龙宗
李玉燕
胡小然
李贵阳
沈巍
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Qingdao Insight Exbio Co ltd
Yantai Fushan District Animal Epidemic Prevention And Control Center
Shandong Yisheng Livestock & Poultry Breeding Co ltd
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Qingdao Insight Exbio Co ltd
Yantai Fushan District Animal Epidemic Prevention And Control Center
Shandong Yisheng Livestock & Poultry Breeding Co ltd
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G01N33/56916—Enterobacteria, e.g. shigella, salmonella, klebsiella, serratia
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38—Diluting, dispersing or mixing samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00465—Separating and mixing arrangements
    • G01N2035/00534—Mixing by a special element, e.g. stirrer
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The application discloses a chicken white diarrhea source phage detection device, which relates to the technical field of chicken white diarrhea detection, wherein four upright posts are fixedly arranged at the top of a base, the top of each upright post is fixedly provided with a same flat plate, two symmetrically arranged cylinders are fixedly arranged at the bottom of each flat plate, the telescopic ends of the two cylinders are fixedly provided with a same long plate, a fourth air pipe rotationally connected with the long plate is arranged on the long plate in a penetrating way, one end of the fourth air pipe, which is positioned below the long plate, is fixedly provided with a rotary table, the top of each flat plate is fixedly provided with a positioning frame, and glass slides are placed in the positioning frames. The application can automatically realize the mixing of the serum solution and the antigen solution, has simple and convenient operation, can simultaneously quantitatively add three groups of serum solutions, does not need to be manually operated to suck for a plurality of times, has high degree of automation, and can automatically stir and mix the two solutions after the serum solution and the antigen solution are mixed.

Description

Pullorum source phage detection device
Technical Field
The application relates to the technical field of pullorum detection, in particular to a pullorum source phage detection device.
Background
Pullorum disease is an infectious disease caused by salmonella pullorum, which is one of the most serious diseases for chicken industry, salmonella is mainly transmitted by hatching eggs, so that in order to reduce loss, a breeding farm often needs to periodically quarantine breeding hens, and a pullorum source phage detection device is needed, for example, a device for pullorum detection is proposed in Chinese patent number CN 206020431U.
At present, when detecting pullorum source phage, three groups of quantitative antigen solutions are firstly dripped into grooves of a slide plate, then quantitative standard positive serum solution, standard negative serum solution and serum solution to be detected are added into the three groups of antigen solutions step by manual work, after the standard positive serum solution, the standard negative serum solution and the serum solution to be detected are added, the two solutions are manually stirred and mixed, then the mixed result of the serum solution to be detected and the antigen solution is observed and is simultaneously compared with the mixed result of other two groups of mixed solutions, so that the conclusion is drawn that the whole operation is troublesome, the time and the labor are wasted, the automation degree is low, the quantitative addition of the three groups of serum solutions is difficult to be carried out simultaneously, and the working efficiency is reduced.
Disclosure of Invention
The application aims to solve the defects in the prior art and provides a pullorum source phage detection device.
In order to achieve the above purpose, the present application adopts the following technical scheme:
the utility model provides a pullorum source phage detection device, includes the base, the top fixed mounting of base has four pole setting, four the top fixed mounting of pole setting has same dull and stereotyped, the bottom fixed mounting of dull and stereotyped has two cylinders of symmetry setting, two fixed mounting has same longeron on the flexible end of cylinder, run through on the longeron and be provided with rather than rotating the fourth trachea of being connected, be provided with slewing mechanism on the longeron, the one end fixed mounting that the fourth trachea is located under the longeron has the carousel, the top fixed mounting of base has the locating frame, the slide has been placed in the locating frame, be equipped with the recess on the slide, the top fixed mounting of locating frame has the magnet that is located the slide both sides, the top fixed mounting of base has three location section of thick bamboo, the bottom fixed mounting of carousel has first installation piece and second installation piece, be provided with three water-absorbing mechanism between first installation piece and the second installation piece, be provided with clamping mechanism on the first installation piece, the top of carousel is provided with the cross trachea and is located the one end fixed mounting of long and has the carousel under the longeron, the three water-absorbing sleeve thread sleeve is located the thread sleeve, the thread is located the three thread sleeve is evenly in the thread sleeve is located respectively.
Preferably, the rotating mechanism comprises a servo motor fixedly mounted on the long plate, an output shaft of the servo motor is fixedly connected with a rotating shaft, a first gear is fixedly mounted on the outer side wall of the rotating shaft, a second gear is fixedly mounted on the outer side wall of one end of the fourth air pipe, which is positioned on the long plate, and the first gear and the second gear are in meshed connection.
Preferably, the liquid suction mechanism comprises a fixed block arranged between a first installation block and a second installation block, a rectangular groove is formed in the fixed block, a limiting block is arranged in the rectangular groove in a penetrating mode, two sides of the limiting block are close to the side wall of the rectangular groove, two supporting rods which are symmetrically arranged are fixedly installed on the limiting block, the fixed block is arranged between the two supporting rods, convex holes are formed in two side walls of the limiting block, installation grooves are formed in two side inner walls of the rectangular groove, a first spring is fixedly installed on one end inner wall of the installation groove, a lug which is in sliding connection with the inner wall of the installation groove is fixedly installed at one end, two ends, close to the limiting block, of the first spring are mutually matched with the convex holes, a suction nozzle which is communicated with the rectangular groove is embedded in the fixed block, a liquid suction sliding plug which is in sliding connection with the inner wall of the suction nozzle is arranged in the suction nozzle, and a connecting rod is fixedly connected between the liquid suction sliding plug and the limiting block.
Preferably, the clamping mechanism comprises a threaded rod which penetrates through the first mounting block and is in threaded connection with the threaded rod, one end of the threaded rod, which is close to the second mounting block, is rotationally connected with a supporting block, a knob is fixedly arranged at one end, which is far away from the second mounting block, of the threaded rod, the supporting block is in sliding connection with the bottom of the turntable, and three pairs of positioning blocks are fixedly arranged on one side wall, which is close to the second mounting block, of the supporting block.
Preferably, the stirring mechanism is including being located stirring a section of thick bamboo and with stirring a section of thick bamboo inner wall sliding connection's hole piece, run through on the hole piece be provided with rather than rotating the stirring pole of being connected, the one end fixed mounting who stirs the pole and be located on the hole piece has helical blade, the venthole has been seted up to the bottom of stirring a section of thick bamboo, the one end that stirs the pole and be located under the hole piece runs through the venthole and extends to the outside of stirring a section of thick bamboo, the one end fixed mounting who stirs the pole and be located outside the stirring a section of thick bamboo has the button, the bottom of button is equipped with evenly distributed's a plurality of mixing bars, two second springs of fixedly connected with symmetry setting between hole piece and the interior bottom of stirring a section of thick bamboo.
Preferably, the air inlet mechanism comprises an inflator pump fixedly arranged at the bottom of the flat plate, an inflation sliding plug which is in sliding connection with the inner wall of the inflator pump is arranged in the inflator pump, an inflation rod is fixedly connected between the inflation sliding plug and the long plate, a second ventilation pipe and a third ventilation pipe which are communicated with the inside of the inflator pump are arranged on the inflator pump, one ends of the second ventilation pipe and the second ventilation pipe, which are communicated with the inflator pump, are located above the inflation sliding plug, one-way valves are arranged in the second ventilation pipe and the third ventilation pipe, the top of the flat plate is fixedly provided with the ventilator pump, the inside of the ventilator pump is provided with the ventilation sliding plug which is in sliding connection with the inside of the ventilator pump, a third spring is fixedly connected between the top of the ventilation sliding plug and the top of the ventilator pump, a through hole is formed in the top of the ventilator pump, one end of the second ventilation pipe, which is far away from the inflator pump, is communicated with the ventilator pump, is located below the ventilation sliding plug, the bottom of the ventilator pump is provided with a first ventilation pipe which is communicated with the inside of the ventilator pump, and the first ventilation pipe is provided with an electromagnetic valve.
Preferably, the hole block is a rectangular block, and the supporting rod and the round block are both made of magnetic materials.
Preferably, one end of the first vent pipe, which is far away from the vent cylinder, is provided with a rotary joint, one end of the fourth vent pipe, which is positioned on the long plate, is connected with the rotary joint, a first conductive block is arranged at the bottom of the long plate, a second conductive block is arranged at the top of the turntable, and the first conductive block and the second conductive block are both positioned in a circuit of the electromagnetic valve.
The application has the beneficial effects that:
(1) The quantitative standard positive serum solution, the standard negative serum solution and the serum solution to be detected can be automatically sucked through the arrangement of the air cylinder, the long plate, the fourth air pipe, the rotating mechanism and the liquid suction mechanism, and then the sucked standard positive serum solution, standard negative serum solution and serum solution to be detected can be automatically extruded into three grooves in a glass slide respectively, so that the mixing of the serum solution and the antigen solution is automatically realized, and the quantitative serum solution suction device is simple and convenient to operate.
(2) By arranging the stirring mechanism and the air inlet mechanism, after the serum solution and the antigen solution are mixed, the two solutions can be automatically stirred and mixed, and compared with the prior art, the application further improves the automation degree, improves the working efficiency and has compact overall structure;
(3) Through setting up clamping mechanism and thread bush, can carry out quick installation and dismantlement to the spare part of imbibition part and stirring part, easily detect next group pullorum source phage, the practicality is stronger.
Drawings
FIG. 1 is a schematic diagram of a perspective structure of a pullorum source phage detection apparatus according to the present application;
FIG. 2 is a schematic diagram showing the perspective structure of the other side of the pullorum disease source phage detection apparatus according to the present application;
FIG. 3 is a schematic plan view of the inside of the mounting groove of the present application;
FIG. 4 is a schematic plan view of the interior of the suction nozzle of the present application;
FIG. 5 is a schematic plan view of the interior of the mixing drum of the present application;
FIG. 6 is a schematic plan view of an air intake mechanism according to the present application;
FIG. 7 is a top view of the present application after placement of the slide;
fig. 8 is a schematic plan view of the abutting block, the positioning block and the fixing block of the present application.
In the figure: 1. a base; 2. a vertical rod; 3. a flat plate; 4. a servo motor; 5. a first gear; 6. a rotating shaft; 7. a ventilation cylinder; 8. a first vent pipe; 9. a second vent pipe; 10. a second gear; 11. an inflator; 12. a cylinder; 13. an inflation rod; 14. a long plate; 15. a first conductive block; 16. a turntable; 17. a magnet; 18. a glass slide; 19. a positioning frame; 20. a stirring barrel; 21. a positioning cylinder; 22. a fixed block; 23. a second conductive block; 24. a communicating pipe; 25. a threaded rod; 26. a first mounting block; 27. a tightening block; 28. a second mounting block; 29. a knob; 30. rectangular grooves; 31. a mounting groove; 32. a first spring; 33. a bump; 34. a convex hole; 35. a limiting block; 36. a supporting rod; 37. a suction nozzle; 38. a liquid suction sliding plug; 39. a connecting rod; 40. a thread sleeve; 41. a helical blade; 42. a hole block; 43. stirring the mixture uniformly; 44. a second spring; 45. round blocks; 46. a mixing rod; 47. a third gas-passing pipe; 48. a third spring; 49. a through hole; 50. a vent plug; 51. an electromagnetic valve; 52. an inflatable sliding plug; 53. a fourth air pipe; 54. a positioning block; 55. and an air outlet hole.
Detailed Description
The following description of the embodiments of the present application will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present application, but not all embodiments.
Referring to fig. 1-8, a pullorum source phage detection device comprises a base 1, wherein four upright posts 2 are fixedly arranged at the top of the base 1, the same flat plate 3 is fixedly arranged at the top of the four upright posts 2, two symmetrically arranged cylinders 12 are fixedly arranged at the bottom of the flat plate 3, the same long plate 14 is fixedly arranged at the telescopic ends of the two cylinders 12, a fourth air pipe 53 which is rotationally connected with the long plate 14 is arranged on the long plate 14 in a penetrating manner, a rotating mechanism is arranged on the long plate 14 and comprises a servo motor 4 which is fixedly arranged on the long plate 14, a rotating shaft 6 is fixedly connected with an output shaft of the servo motor 4, a first gear 5 is fixedly arranged on the outer side wall of the rotating shaft 6, a second gear 10 is fixedly arranged on the outer side wall of one end of the fourth air pipe 53 which is positioned on the long plate 14, and the first gear 5 is in meshed connection with the second gear 10;
the turntable 16 is fixedly arranged at one end of the fourth air pipe 53 positioned below the long plate 14, the positioning frame 19 is fixedly arranged at the top of the base 1, the glass slide 18 is arranged in the positioning frame 19, the glass slide 18 is provided with a groove, the magnets 17 positioned at two sides of the glass slide 18 are fixedly arranged at the top of the positioning frame 19, the three positioning cylinders 21 are fixedly arranged at the top of the base 1, the first mounting block 26 and the second mounting block 28 are fixedly arranged at the bottom of the turntable 16, three liquid absorbing mechanisms are arranged between the first mounting block 26 and the second mounting block 28 and are mutually connected into a whole, the liquid absorbing mechanisms are convenient to mount and dismount, each liquid absorbing mechanism comprises a fixed block 22 arranged between the first mounting block 26 and the second mounting block 28, a rectangular groove 30 is formed in the fixed block 22, a limiting block 35 is penetratingly arranged in the rectangular groove 30, two sides of the limiting block 35 are close to the side wall of the rectangular groove 30, two supporting rods 36 which are symmetrically arranged are fixedly arranged on the limiting block 35, the fixed block 22 is positioned between the two supporting rods 36, convex holes 34 are formed in the two side walls of the limiting block 35, mounting grooves 31 are formed in the inner walls of the two sides of the rectangular groove 30, first springs 32 are fixedly arranged on the inner walls of one end of each of the two mounting grooves 31, a bump 33 which is in sliding connection with the inner wall of the corresponding mounting groove 31 is fixedly arranged at one end, close to the limiting block 35, of each first spring 32, the bump 33 is mutually matched with the convex holes 34, a suction nozzle 37 which is communicated with the rectangular groove 30 is embedded on the fixed block 22, a liquid suction sliding plug 38 which is in sliding connection with the inner wall of the suction nozzle 37 is arranged in the suction nozzle 37, and a connecting rod 39 is fixedly connected between the liquid suction sliding plug 38 and the limiting block 35;
the clamping mechanism is arranged on the first mounting block 26 and comprises a threaded rod 25 which penetrates through the first mounting block 26 and is in threaded connection with the first mounting block, one end of the threaded rod 25, which is close to the second mounting block 28, is rotationally connected with a supporting block 27, one end of the threaded rod 25, which is far away from the second mounting block 28, is fixedly provided with a knob 29, the supporting block 27 is in sliding connection with the bottom of the turntable 16, and three pairs of positioning blocks 54 are fixedly arranged on one side wall, which is close to the second mounting block 28, of the supporting block 27;
three communicating pipes 24 communicated with a fourth air pipe 53 are arranged above the rotary table 16, three threaded sleeves 40 are fixedly arranged at the bottom of the rotary table 16, one ends of the three communicating pipes 24 far away from the fourth air pipe 53 penetrate through the rotary table 16 and are located right above the adjacent threaded sleeves 40, a stirring barrel 20 is fixedly connected to one ends of the three threaded sleeves 40, stirring mechanisms are arranged on the three stirring barrels 20, each stirring mechanism comprises a hole block 42 which is located in the stirring barrel 20 and is in sliding connection with the inner wall of the stirring barrel 20, each hole block 42 is a rectangular block, stirring rods 43 which are in rotary connection with the hole block 42 are penetrated through the hole block 42, a helical blade 41 is fixedly arranged at one end of each stirring rod 43 located on each hole block 42, an air outlet hole 55 is formed at the bottom of the stirring barrel 20, round blocks 45 are fixedly arranged at one ends of the stirring rods 43 located outside the stirring barrel 20, each supporting rod 36 and each round block 45 are made of magnetic materials, a plurality of uniformly distributed mixing rods 46 are arranged at the bottom of the round blocks 45, and two symmetrical inner bottoms of the stirring barrel 20 are fixedly connected with two springs 44;
an air inlet mechanism is arranged above the long plate 14, the air inlet mechanism comprises an inflator 11 fixedly arranged at the bottom of the flat plate 3, an inflation sliding plug 52 which is in sliding connection with the inner wall of the inflator 11 is arranged in the inflator 11, an inflation rod 13 is fixedly connected between the inflation sliding plug 52 and the long plate 14, a second vent pipe 9 and a third vent pipe 47 which are communicated with the inside of the inflator 11 are arranged on the inflator 11, one ends of the second vent pipe 9 and the third vent pipe 47 which are communicated with the inflator 11 are positioned above the inflation sliding plug 52, one-way valves are arranged in the second vent pipe 9 and the third vent pipe 47, an air vent cylinder 7 is fixedly arranged at the top of the flat plate 3, an air vent sliding plug 50 which is in sliding connection with the inside of the air vent cylinder 7 is arranged in the air vent cylinder 7, a third spring 48 is fixedly connected between the air vent sliding plug 50 and the top end of the air vent cylinder 7, through hole 49 has been seted up at the top of breather tube 7, the one end that inflator pump 11 was kept away from to second breather tube 9 communicates with breather tube 7, the one end that second breather tube 9 and breather tube 7 communicate is located the below of breather slide 50, the bottom of breather tube 7 is provided with the first breather tube 8 that is linked together rather than inside, be provided with solenoid valve 51 on the first breather tube 8, the one end that breather tube 7 was kept away from to first breather tube 8 is equipped with rotary joint, the one end that fourth breather tube 53 is located long board 14 is connected with rotary joint, the bottom of long board 14 is provided with first conducting block 15, the top of carousel 16 is provided with second conducting block 23, first conducting block 15 and second conducting block 23 all are located solenoid valve 51's circuit.
When the application is used, the glass slide 18 is placed into the positioning frame 19, a certain amount of antigen solution is dripped into three grooves in the glass slide 18, then a standard positive serum solution tank, a standard negative serum solution tank and a serum solution tank to be detected are respectively placed in the three positioning cylinders 21, the three fixing blocks 22 are respectively positioned in the three pairs of positioning blocks 54, the tops of the three fixing blocks 22 are propped against the bottom of the turntable 16, the threaded rod 25 is rotated by rotating the knob 29, the propping block 27 is in sliding connection with the bottom of the turntable 16, so that the propping block 27 moves towards the direction close to the second mounting block 28, the clamping and fixing of the fixing blocks 22 are realized, after the three fixing blocks 22 are clamped and fixed, the three suction nozzles 37 are respectively corresponding to the three positioning cylinders 21 due to the positioning action of the positioning blocks 54, the outer side wall of the stirring cylinder 20 is provided with threads, the stirring cylinder 20 is screwed into the threaded sleeve 40, and the top of the stirring cylinder 20 is tightly propped against the bottom of the turntable 16;
when the long plate 14 moves downwards for the first time by starting the air cylinder 12, the three suction nozzles 37 move downwards and respectively enter the standard positive serum solution tank, the standard negative serum solution tank and the serum solution tank to be detected, and the three suction nozzles 37 move downwards and respectively enter the standard positive serum solution tank, the standard negative serum solution tank and the serum solution tank to be detected, the supporting rod 36 is contacted with the top surface of the positioning cylinder 21, so that the limiting block 35 moves upwards in the rectangular groove 30, finally, the convex holes 34 on the limiting block 35 are matched with the convex blocks 33, the first springs 32 are compressed, and the limiting block 35 moves upwards to drive the connecting rod 39 and the liquid suction sliding plug 38 to move upwards, so that quantitative standard positive serum solution, standard negative serum solution and the serum solution to be detected are respectively sucked into the three suction nozzles 37;
when the long plate 14 moves downwards, the air-filling sliding plug 52 slides downwards through the air-filling rod 13, so that air is sucked into the air-filling cylinder 11 through the third air-filling pipe 47, then when the air cylinder 12 moves the long plate 14 upwards to the initial position (highest position), the air-filling sliding plug 52 slides upwards, so that the air sucked into the air-filling cylinder 11 is extruded into the air-filling cylinder 7 through the second air-filling pipe 9, as the first conducting block 15 and the second conducting block 23 are not contacted with each other, the circuit of the electromagnetic valve 51 cannot be connected, the valve of the electromagnetic valve is in a closed state, the air extruded into the air-filling cylinder 7 cannot pass through the first air-filling pipe 8, so that the air-filling sliding plug 50 slides upwards, the third spring 48 is compressed, meanwhile, because the first spring 32 is compressed, so that the limiting block 35 cannot return to the initial position (lowest position), when the long plate 14 returns to the initial position for the first time, the servo motor 4 rotates the rotating shaft 6 and the first gear 5, and then rotates the fourth air-filling pipe 53 through the second gear 10, so that the three fixed blocks 22 and the three cylinders 20 under the rotating disc 16 rotate around the axis of the turntable 16, so that the three fixed blocks 22 and the three cylinders 20 rotate around the twenty hundred degrees, and the three fixed blocks 22 are positioned on the three evenly-positioned slide 20;
then the air cylinder 12 moves the long plate 14 downwards again, the three suction nozzles 37 move downwards and respectively enter the three grooves, the three suction nozzles 37 move downwards to the process of entering the three grooves respectively, the magnetic attraction force of the magnetic material against the magnet 17 is gradually increased, when the suction nozzles 37 completely enter the grooves, the convex holes 34 on the limiting block 35 are not matched with the convex blocks 33 any more, so that the limiting block 35 returns to the initial position, the connecting rod 39 and the liquid suction sliding plug 38 are driven to slide downwards, the solution sucked into the three suction nozzles 37 is respectively extruded into the three grooves, so that the serum solution is automatically and quantitatively added into the antigen solution, and when the long plate 14 moves downwards to the lowest position again, the mixing rod 46 is still positioned above the standard positive serum solution tank, the standard negative serum solution tank and the serum solution tank to be detected, then the air cylinder 12 moves the long plate 14 downwards again to the initial position, in the process, air is sucked into the inflator 11 again through the third air vent pipe 47 and is extruded into the inflator 7 again through the second air vent pipe 9, the circuit of the electromagnetic valve 51 cannot be connected because the first conductive block 15 and the second conductive block 23 are still not contacted with each other, the valve of the electromagnetic valve 51 is in a closed state, and the air extruded into the inflator 7 cannot pass through the first air vent pipe 8, so that the air vent sliding plug 50 continues to slide upwards, and the third spring 48 is further compressed;
the servo motor 4 is then started again, the three fixing blocks 22 and the three stirring cylinders 20 are rotated again for one hundred twenty degrees, so that the three stirring cylinders 20 are positioned right above the three grooves, when the servo motor 4 is started again according to the drawing of fig. 1, the second conductive block 23 is rotated again for one hundred twenty degrees, namely the first conductive block 15 is rotated clockwise for two hundred forty degrees from the position of the drawing of fig. 1, the first conductive block 15 is just positioned, the first conductive block 15 and the second conductive block 23 are contacted with each other, the valve of the electromagnetic valve 51 is opened, after the valve of the electromagnetic valve 51 is in an opened state, the air extruded into the ventilation cylinder 7 passes through the first ventilation pipe 8 and the communication pipe 24 and enters the three stirring cylinders 20 tightly abutted against the turntable 16 by the elastic force generated by the compression of the third spring 48, the high-speed air flow passes through the upper hole of the hole block 42 and is finally ejected from the air outlet hole 55, by providing the spiral blade 41, the high-speed air flow can push the spiral blade 41 to rotate, so as to drive the stirring rod 43, the round block 45 and the mixing rod 46 to rotate, and the high-speed air flow can realize the rotation of the stirring rod 43 in a period of time due to the small air outlet hole 55, the air cylinder 12 can downwards move the long plate 14 again, air is sucked into the inflator 11 through the third air pipe 47 again, the stirring barrel 20 downwards moves, when the stirring barrel 20 downwards moves to reach the lowest position, the round block 45 is gradually increased by the magnetic attraction of the magnet 17, when the stirring barrel 20 downwards moves to the lowest position, the round block 45 and the mixing rod 46 are downwards moved by the magnetic attraction of the magnet 17, the second spring 44 is compressed, the rotating mixing rod 46 finally downwards moves into the groove to stir and mix the antigen solution and the serum solution, when the air cylinder 12 moves the long plate 14 upwards again, air is extruded into the ventilation cylinder 7 again through the second ventilation pipe 9 and is extruded into the stirring cylinder 20 through the first ventilation pipe 8 and the communication pipe 24, the stirring rod 43 rotates again, remixing is achieved, finally the glass slide 18 is taken out, and the mixed solution in the three grooves is observed, so that detection is completed.
The foregoing is only a preferred embodiment of the present application, but the scope of the present application is not limited thereto, and any person skilled in the art, who is within the scope of the present application, should make equivalent substitutions or modifications according to the technical scheme of the present application and the inventive concept thereof, and should be covered by the scope of the present application.

Claims (3)

1. The utility model provides a pullorum source phage detection device, includes base (1), the top fixed mounting of base (1) has four pole settings (2), four the top fixed mounting of pole settings (2) has same flat board (3), its characterized in that, the bottom fixed mounting of flat board (3) has two cylinders (12) of symmetry setting, and two fixed mounting has same longeron (14) on the flexible end of cylinder (12), run through on longeron (14) be provided with fourth trachea (53) rather than rotating connection, be provided with rotary mechanism on longeron (14), one end fixed mounting who is located under longeron (14) of fourth trachea (53) carousel (16), the top fixed mounting of base (1) has locating frame (19), place slide glass (18) in locating frame (19), be equipped with the recess on slide glass (18), the top fixed mounting of locating frame (19) has magnet (17) that are located slide glass (18) both sides, be provided with on the top fixed mounting of base (1) has three locating piece (21) and a third piece (26) and a second fixed mounting of locating piece (28) have between installation carousel (28), the first mounting block (26) is provided with a clamping mechanism, and an air inlet mechanism is arranged above the long plate (14); the rotating mechanism comprises a servo motor (4) fixedly arranged on a long plate (14), an output shaft of the servo motor (4) is fixedly connected with a rotating shaft (6), a first gear (5) is fixedly arranged on the outer side wall of the rotating shaft (6), a second gear (10) is fixedly arranged on the outer side wall of one end of a fourth air pipe (53) positioned on the long plate (14), and the first gear (5) and the second gear (10) are in meshed connection; the pipetting mechanism includes a fixed block (22) disposed between a first mounting block (26) and a second mounting block (28), rectangular grooves (30) are formed in the fixing blocks (22), limiting blocks (35) are arranged in the rectangular grooves (30) in a penetrating mode, two sides of the limiting block (35) are close to the side wall of the rectangular groove (30), two supporting rods (36) which are symmetrically arranged are fixedly arranged on the limiting block (35), the fixed block (22) is positioned between the two supporting rods (36), the two side walls of the limiting block (35) are provided with convex holes (34), mounting grooves (31) are formed in the inner walls of the two sides of the rectangular groove (30), first springs (32) are fixedly mounted in the inner parts of the end parts in the two mounting grooves (31), protruding blocks (33) which are in sliding connection with the inner walls of the mounting grooves (31) are fixedly mounted at one ends, close to limiting blocks (35), of the two first springs (32), the convex blocks (33) are mutually matched with the convex holes (34), the fixed blocks (22) are embedded with suction nozzles (37) communicated with the rectangular grooves (30), a liquid suction sliding plug (38) which is connected with the inner wall of the suction nozzle (37) in a sliding way is arranged in the suction nozzle, a connecting rod (39) is fixedly connected between the liquid suction sliding plug (38) and the limiting block (35); the clamping mechanism comprises a threaded rod (25) which is arranged on a first mounting block (26) in a penetrating mode and is in threaded connection with the threaded rod, one end, close to a second mounting block (28), of the threaded rod (25) is rotationally connected with a supporting and tightening block (27), a knob (29) is fixedly arranged at one end, far away from the second mounting block (28), of the threaded rod (25), the supporting and tightening block (27) is in sliding connection with the bottom of the turntable (16), and three pairs of positioning blocks (54) are fixedly arranged on one side wall, close to the second mounting block (28), of the supporting and tightening block (27); three communicating pipes (24) which are communicated with a fourth air pipe (53) are arranged above the rotary table (16), three thread sleeves (40) are fixedly arranged at the bottom of the rotary table (16), one ends of the three communicating pipes (24) which are far away from the fourth air pipe (53) are respectively positioned in the adjacent thread sleeves (40), the three thread sleeves (40) are internally and respectively connected with an air outlet hole (55) in a threaded manner, the three air outlet hole (20) are respectively provided with an evenly stirring mechanism, the evenly stirring mechanism comprises a hole block (42) which is positioned in the air outlet hole (20) and is in sliding connection with the inner wall of the air outlet hole (20), evenly stirring rods (43) which are rotationally connected with the hole block (42) are arranged on the hole block (42), spiral blades (41) are fixedly arranged at one ends of the evenly stirring rods (43) which are positioned on the hole block (42), air outlet holes (55) are formed in the bottom of the air outlet hole (55) are respectively arranged at one ends of the evenly stirring rods (43) which are positioned under the hole block (42) and extend to the outer side of the air outlet hole (20), the evenly stirring rods (43) are positioned at the outer sides of the air outlet hole (20), a plurality of round pieces (45) are arranged at the bottom of the evenly stirring rod (45), two second springs (44) which are symmetrically arranged are fixedly connected between the hole block (42) and the inner bottom of the stirring cylinder (20); the air inlet mechanism comprises an air charging barrel (11) fixedly arranged at the bottom of a flat plate (3), an air charging sliding plug (52) which is in sliding connection with the inner wall of the air charging barrel (11) is arranged in the air charging barrel (11), an air charging rod (13) is fixedly connected between the air charging sliding plug (52) and a long plate (14), a second air ventilation pipe (9) and a third air ventilation pipe (47) which are communicated with the air charging barrel are arranged on the air charging barrel (11), one ends of the second air ventilation pipe (9) and the third air ventilation pipe (47) are all positioned above the air charging sliding plug (52), one-way valves are arranged in the second air ventilation pipe (9) and the third air ventilation pipe (47), an air ventilation barrel (7) is fixedly arranged at the top of the flat plate (3), an air ventilation sliding plug (50) which is in sliding connection with the inside of the air charging barrel (7) is arranged in the air charging barrel, a third spring (48) is fixedly connected between the air ventilation sliding plug (50) and the top of the air charging barrel (7), a through hole (49) is formed in the top of the air charging barrel (7), one end of the second air ventilation pipe (9) and one end (7) which is communicated with the air charging barrel (7) is far away from the air charging barrel (7), an electromagnetic valve (51) is arranged on the first vent pipe (8).
2. The pullorum source phage detection apparatus as claimed in claim 1, wherein the hole block (42) is a rectangular block, and the abutment bar (36) and the round block (45) are both magnetic materials.
3. The pullorum source phage detection apparatus as claimed in claim 2, wherein the end of the first ventilation pipe (8) far away from the ventilation pipe (7) is provided with a rotary joint, the end of the fourth ventilation pipe (53) located on the long plate (14) is connected with the rotary joint, the bottom of the long plate (14) is provided with a first conductive block (15), the top of the turntable (16) is provided with a second conductive block (23), and the first conductive block (15) and the second conductive block (23) are both located in the circuit of the electromagnetic valve (51).
CN202310749190.6A 2023-06-25 2023-06-25 Pullorum source phage detection device Active CN116500265B (en)

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CN202310749190.6A CN116500265B (en) 2023-06-25 2023-06-25 Pullorum source phage detection device

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CN202310749190.6A CN116500265B (en) 2023-06-25 2023-06-25 Pullorum source phage detection device

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