CN116426384A - Cell culture device for biological analysis - Google Patents

Abstract

The invention relates to the technical field of cell culture and discloses a cell culture device for biological analysis, which comprises a cell culture device body, wherein a box door is rotatably assembled on the cell culture device body, the inner surface of the cell culture device body is fixedly connected with a partition plate, the partition plate divides the interior of the cell culture device body into a culture area and a material taking area, a through hole is formed in the bottom end position of the partition plate, and a plurality of placing cylinders are assembled in the cell culture device body. When the cell culture device provided by the invention is used for taking materials, the separating mechanism and the taking mechanism can prevent the temperature of the culture area in the cell culture device body from being outwards scattered, so that the temperature of the culture area in the cell culture device body is prevented from being greatly fluctuated, the constant temperature effect of the culture area in the cell culture device body is ensured, and the cell culture effect of the cell culture device body is ensured.

Description

A cell culture device for biological analysis

technical field

The invention relates to the technical field of cell culture, in particular to a cell culture device for biological analysis.

Background technique

Cell culture refers to a method of simulating the internal environment in vitro to enable it to survive, grow, reproduce and maintain its main structure and function. Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. No matter for the entire bioengineering technology or one of the biological cloning technologies, cell culture is an essential process, and cell culture itself is a large-scale cloning of cells. Cell culture technology can transform a single cell into simple single cell or rarely differentiated multi-cell through mass culture, which is an essential part of cloning technology, and cell culture itself is cell cloning. Cell culture technology is an important and commonly used technology in cell biology research methods. Through cell culture, a large number of cells can be obtained, and it can also be used to study cell signal transduction, cell anabolism, and cell growth and proliferation.

The prior art (CN106434343B) discloses a remote biological cell culture device and auxiliary system and its application, which relates to a remote monitoring and early warning biological cell growth environment auxiliary system and its application. Limited to the control of temperature and humidity, it cannot conduct network collection and detailed chart analysis of data by itself, and does not have the function of real-time transmission monitoring and early warning of biological cells. The above technical solution is provided by setting silver ion partitions in the cavity , gas concentration sensor, water level sensor, temperature and humidity sensor, oxygen sensor and conductivity sensor, and realize remote monitoring of the biological cell culture process by connecting the single-board computer remote switch control system with the terminal monitoring and early warning software. The system of the above technical scheme is used For cultivating cells or viruses, microorganisms and plant cells derived from mammals, however, the cell culture device proposed by the above-mentioned technical scheme also has the following disadvantages in actual use: when the staff needs to take out the culture bottle from the inside of the cell culture device, First of all, the door of the device must be opened to take out the culture bottle from the device. Since cell culture needs to be carried out in a constant temperature space, every time the door is opened, the internal temperature of the device will change. Although the cell culture device usually All have a temperature regulation function, but frequent temperature changes are not conducive to the normal growth of cells and cannot meet the growth needs of cells.

Therefore, it is necessary to design a cell culture device for bioanalysis to solve the above problems.

Contents of the invention

The purpose of the present invention is to solve the shortcomings in the prior art, and propose a cell culture device for biological analysis.

In order to achieve the above object, the present invention adopts the following technical solutions:

A cell culture device for biological analysis, comprising a cell culture device body, the cell culture device body is rotatably equipped with a box door, and the interior of the cell culture device body is divided into a culture area and a material retrieving area by a partition plate. The bottom of the partition plate is provided with a port, and the inside of the cell culture device body is equipped with several placement cylinders, one of which is located in the feeding area, and the rest of the placement cylinders are located in the cultivation area. Inside;

A partition mechanism is provided on the partition plate, and the partition mechanism includes a lifting assembly and a sealing assembly;

The inside of the cell culture device body is provided with a retrieving mechanism, and the retrieving mechanism includes a moving component 1, a moving component 2 and a moving component 3;

The inside of the cell culture device body is also provided with a clamping assembly;

A fixing mechanism is provided on several of the placing cylinders, and the fixing mechanism includes a clamping assembly and a triggering assembly.

As a preferred technical solution of the present invention, the lifting assembly includes a fixed frame, a cylinder one, a lifting seat, a guide rail and a sealed door panel, the fixed frame is fixedly connected to the partition plate, and the cylinder one is installed on a fixed On the frame, the lifting seat is fixedly connected to the telescopic end of cylinder one, the guide rail is fixedly connected to the partition plate, the lifting seat is slidably fitted on the guide rail, and the sealed door panel is fixedly connected to the lifting seat on, and the sealing door is slidably fitted in the opening.

As a preferred technical solution of the present invention, the sealing assembly includes four sealing sheets, sealing strip one, several grooves, sealing strip two and several bumps, and the four sealing sheets are set in pairs and fixed respectively Connected to the two facing inner surfaces of the port, the four sealing sheets are respectively located on both sides of the sealing door panel, and the four sealing sheets are all attached to the sealing door panel, and the sealing strip is fixedly connected to the sealing door panel On the bottom surface, several of the grooves are set on the first sealing strip, and the second sealing strip is fixedly connected to the inner bottom surface of the port, and some of the protrusions are fixedly connected to the second sealing strip, and several of the protrusions are respectively Set for several grooves.

As a preferred technical solution of the present invention, the first moving assembly includes a first fixed seat, a first sliding seat and a first drive motor, the first fixed seat is fixedly connected to the inner surface of the cell culture device body, and the first fixed seat A sliding seat is slidably fitted on the first fixed seat, the first driving motor is mounted on the first sliding seat, and the first driving motor is used to drive the first sliding seat to move on the first fixed seat.

As a preferred technical solution of the present invention, the second moving assembly includes a second fixed seat, a second drive motor and a second sliding seat, the second fixed seat is fixedly connected to the first sliding seat, and the second The sliding seat is slidably fitted on the second fixed seat, and the second driving motor is installed on the second sliding seat.

As a preferred technical solution of the present invention, the moving assembly three includes a lifting slider, an assembly frame and a cylinder two, the lifting slider is slidably assembled on the second sliding seat, and the assembly frame is fixedly connected to the lifting slider , the second cylinder is installed on the assembly frame.

As a preferred technical solution of the present invention, the clamping assembly includes a clamping seat, several fixing plates, three jaws, three shafts, three worm gears, a servo motor and a worm, and the clamping seat is fixedly connected On the telescopic end of the cylinder two, several of the fixed plates are fixedly connected to the clamping seat, and the three shafts are all rotatably connected between the fixed plates facing each other, and the three jaws are fixed respectively. Sleeved on the three shafts, the three worm wheels are respectively fixedly sleeved on the three shafts, the servo motor is installed on the clamping seat, the worm is fixedly connected to the telescopic end of the servo motor, and the worm Mesh with three worm gears.

As a preferred technical solution of the present invention, the clamping assembly includes a sliding plate, a base, a limiting groove, two slots, two clamping plates, four sliding slots and four sliding blocks, the sliding plate Slidingly assembled on the inner surface of the placement cylinder, the base is fixedly connected to the top surface of the sliding plate, the limiting groove is opened on the base, the two slots are opened on the inner surface of the placement cylinder, the two clamping The plates are slidably connected in the two slots respectively, and the four chute slots are set in pairs in pairs, and are set on the inner surfaces of the two slots respectively, and the four sliders are in groups of two, and are respectively fixedly connected in the two slots. on a clamping plate, and the four sliders are slidably fitted in the four chute respectively.

As a preferred technical solution of the present invention, the trigger assembly includes an airbag one, a spring one, two airbags two and two springs two, the airbag one is arranged between the inner bottom surface of the placement cylinder and the sliding plate, two The two airbags are respectively arranged between the two slots and the two clamping plates, the spring one is assembled inside the airbag one, and the two springs two are respectively assembled inside the two airbags two, and the two springs are respectively assembled inside the two airbags two. Both the air bag two and the air bag one are connected through a trachea.

As a preferred technical solution of the present invention, anti-skid pads are bonded to the three jaws.

The present invention has the following beneficial effects:

By setting the partition plate, the partition mechanism and the material retrieving mechanism, under the cooperation of the partition plate, the separation mechanism and the material reclaiming mechanism, the clamping assembly can place the culture bottle that needs to be taken out into the placement cylinder in the material retrieving area, Then the sealed door panel can completely cover the opening, which can prevent the temperature of the culture area in the cell culture device body from escaping outwards, thereby avoiding large fluctuations in the temperature of the culture area in the cell culture device body, and ensuring the temperature of the culture area in the cell culture device body. Constant temperature effect, so as to ensure the cell culture effect of the cell culture device body;

By setting the fixing mechanism, when the culture bottle falls into the placement cylinder, the first airbag can press the internal gas into the two airbags through the two tracheas, so that the two airbags are inflated, and the two airbags can be driven when they are inflated. The two clamping plates are close to each other, so that the two clamping plates clamp the culture bottle, and can fix the culture bottle in the placement cylinder to ensure the stability of the culture bottle in the placement cylinder.

Description of drawings

Fig. 1 is a schematic structural view of a cell culture device for biological analysis proposed by the present invention;

Fig. 2 is a structural schematic diagram of a cell culture device for biological analysis proposed by the present invention when the door is opened;

Fig. 3 is a schematic cross-sectional structure diagram of a cell culture device for biological analysis proposed by the present invention;

Fig. 4 is a schematic cross-sectional structure diagram of a cell culture device for biological analysis proposed by the present invention;

Fig. 5 is a structural schematic diagram of a partition mechanism in a cell culture device for biological analysis proposed by the present invention;

Fig. 6 is a schematic structural view of a retrieving mechanism in a cell culture device for biological analysis proposed by the present invention;

7 is a schematic structural view of a clamping assembly in a cell culture device for biological analysis proposed by the present invention;

Fig. 8 is a schematic structural diagram of a fixing mechanism in a cell culture device for biological analysis proposed by the present invention.

In the figure: 1 cell culture device body, 2 box door, 3 partition plate, 4 port, 5 placement cylinder, 6 partition mechanism, 61 lifting component, 611 fixing frame, 612 cylinder one, 613 lifting seat, 614 guiding slide rail , 615 sealed door panel, 62 sealed assembly, 621 sealed sheet, 622 sealed strip 1, 623 groove, 624 sealed strip 2, 625 bump, 7 feeding mechanism, 71 moving component 1, 711 first fixed seat, 712 first Sliding seat, 713 first driving motor, 72 moving assembly two, 721 second fixed seat, 722 second driving motor, 723 second sliding seat, 73 moving assembly three, 731 lifting slider, 732 assembly frame, 733 cylinder two, 8 clamping assembly, 81 clamping seat, 82 fixed plate, 83 jaw, 84 shaft, 85 worm gear, 86 servo motor, 87 worm, 9 fixing mechanism, 91 clamping component, 911 sliding plate, 912 base, 913 limit Position slot, 914 slotting, 915 clamping plate, 916 chute, 917 slide block, 92 trigger assembly, 921 air bag one, 922 spring one, 923 air bag two, 924 spring two.

Implementation

The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention.

Referring to Figures 1-8, a cell culture device for biological analysis includes a cell culture device body 1 on which a door 2 is rotatably mounted, and a partition plate 3 is fixedly connected to the inner surface of the cell culture device body 1, The partition plate 3 divides the interior of the cell culture device body 1 into a culture area and a material retrieving area. The bottom end of the partition plate 3 is provided with a port 4, and the cell culture device body 1 is equipped with a plurality of placement cylinders 5, wherein One placing cylinder 5 is located in the material fetching area, and the remaining multiple placing cylinders 5 are all located in the cultivating area;

A partition mechanism 6 is provided on the partition plate 3, and the partition mechanism 6 includes a lifting assembly 61 and a sealing assembly 62;

The interior of the cell culture device body 1 is provided with a retrieving mechanism 7, and the retrieving mechanism 7 includes a moving component 1 71, a moving component 2 72 and a moving component 3 73;

The inside of the cell culture device body 1 is also provided with a clamping assembly 8;

A fixing mechanism 9 is provided on several placing cylinders 5 , and the fixing mechanism 9 includes a clamping component 91 and a triggering component 92 .

Referring to Fig. 5, the lifting assembly 61 includes a fixed frame 611, a cylinder one 612, a lifting seat 613, a guide rail 614 and a sealed door panel 615, the fixed frame 611 is fixedly connected on the partition plate 3, and the cylinder one 612 is installed on the fixed frame 611 , the lift seat 613 is fixedly connected to the telescopic end of the cylinder one 612, the guide rail 614 is fixedly connected to the partition plate 3, the lift seat 613 is slidably assembled on the guide rail 614, and the sealing door panel 615 is fixedly connected to the lift seat 613, And the sealing door panel 615 is slidably fitted in the opening 4 , and the lifting assembly 61 is provided to facilitate the upward and downward movement of the sealing door panel 615 .

Referring to Fig. 5, the sealing assembly 72 includes four sealing sheets 621, a sealing strip 622, a plurality of grooves 623, a sealing strip 2 624 and a plurality of protrusions 625, and the four sealing sheets 621 are in groups of two, and are respectively fixedly connected to the On the two facing inner surfaces of the port 4, four sealing sheets 621 are respectively located on both sides of the sealing door panel 615, and the four sealing sheets 621 are all attached to the sealing door panel 615, and the sealing strip 1 622 is fixedly connected to the sealing door panel 615. On the bottom surface, several grooves 623 are set on the first sealing strip 622, the second sealing strip 624 is fixedly connected to the inner bottom surface of the port 4, and several protrusions 625 are fixedly connected on the second sealing strip 624, and the plurality of protrusions 625 are respectively facing Several grooves 623 are provided, and sealing strip 1 622 and sealing strip 2 624 can increase the contact area between the sealing door panel 615 and the port 4 to ensure the sealing performance of the joint between the sealing door panel 615 and the port 4. Several bumps 625 and some Groove 623 can increase the contact area between sealing strip 1 622 and sealing strip 2 624, can increase the contact area between sealing strip 1 622 and sealing strip 2 624, and further ensure the sealing of the joint between sealing door panel 615 and port 4 performance, preventing heat from leaking through the connection between the sealing door panel 615 and the port 4.

Referring to Fig. 6, the moving assembly 1 71 includes a first fixed seat 711, a first sliding seat 712 and a first drive motor 713, the first fixed seat 711 is fixedly connected to the inner surface of the cell culture device body 1, and the first sliding seat 712 is slidably assembled On the first fixed base 711 , a first driving motor 713 is installed on the first sliding base 712 , and the first driving motor 713 is used to drive the first sliding base 712 to move on the first fixing base 711 .

Referring to Fig. 6, the moving assembly 2 72 includes a second fixed seat 721, a second drive motor 722 and a second sliding seat 723, the second fixed seat 721 is fixedly connected to the first sliding seat 712, and the second sliding seat 723 is slidably assembled on On the second fixing seat 721 , the second driving motor 722 is installed on the second sliding seat 723 .

With reference to Fig. 6, moving assembly three 73 comprises lift slide block 731, assembly frame 732 and cylinder two 733, and lift slide block 731 is slidingly assembled on the second slide seat 723, and assembly frame 732 is fixedly connected on the lift slide block 731, and cylinder two 733 Installed on the mounting bracket 732.

7, the clamping assembly 8 includes a clamping seat 81, several fixed plates 82, three jaws 83, three shafts 84, three worm gears 85, a servo motor 86 and a worm 87, and the clamping seat 81 is fixedly connected to On the telescopic end of cylinder 2 733, several fixed plates 82 are all fixedly connected on the clamping seat 81, and the three shaft rods 84 are all rotatably connected between the two facing fixed plates 82, and the three clamping jaws 83 are respectively fixed sleeves. Set on the three shafts 84, the three worm gears 85 are respectively fixedly sleeved on the three shafts 84, the servo motor 86 is installed on the clamping seat 81, the worm 87 is fixedly connected to the telescopic end of the servo motor 86, and the worm 87 and the three worm gears 85 mesh with each other, and by setting the clamping assembly 8, it is convenient to clamp the culture bottle.

Referring to Fig. 8, the clamping assembly 91 includes a sliding plate 911, a base 912, a limiting groove 913, two slots 914, two clamping plates 915, four sliding grooves 916 and four sliding blocks 917, and the sliding plate 911 slides Assembled on the inner surface of the placement tube 5, the base 912 is fixedly connected to the top surface of the sliding plate 911, the limit groove 913 is opened on the base 912, two slots 914 are opened on the inner surface of the placement tube 5, and the two clamping plates 915 Slidingly connected in the two slots 914 respectively, the four slide slots 916 are set in pairs, and are set on the inner surfaces of the two slots 914 respectively, and the four sliders 917 are connected in groups of two, and are fixedly connected to the two slots 914 respectively. On the clamping plate 915, and the four sliders 917 are slidably fitted in the four chutes 916 respectively. By setting the clamping assembly 91, the two clamping plates 915 can clamp the culture bottle, and the culture bottle can be fixed on the Place in the cylinder 5 to ensure the stability of the culture bottle in the cylinder 5.

Referring to Fig. 8, the trigger assembly 92 includes an airbag one 921, a spring one 922, two airbag two 923 and two spring two 924, the airbag one 921 is arranged between the inner bottom surface of the placement cylinder 5 and the sliding plate 911, and the two airbag two 923 are respectively arranged between the two slots 914 and the two clamping plates 915, the spring one 922 is assembled inside the airbag one 921, the two spring two 924 are respectively assembled inside the two airbag two 923, and the two airbag two Both the 923 and the air bag 1 921 are connected through a trachea, and the trigger assembly 92 is provided to facilitate the two clamping plates 915 to approach each other.

Referring to FIG. 7 , the three jaws 83 are all bonded with anti-slip pads, and such arrangement can ensure the stability of the three jaws 83 for clamping the culture bottle.

Concrete working principle of the present invention is as follows:

In the initial state, the sealing door 615 completely blocks the port 4. The sealing door 615 can prevent the temperature in the culture area of the cell culture device body 1 from escaping outward through the port 4. When the sealing door 615 completely blocks the port 4 At the same time, several protrusions 625 on the second sealing strip 624 can be snapped into several grooves 623 on the first sealing strip 622, and the first sealing strip 622 and the second sealing strip 624 can increase the contact between the sealing door panel 615 and the port 4 Area, to ensure the sealing performance of the joint between the sealing door panel 615 and the port 4, several bumps 625 and several grooves 623 can increase the contact area between the first sealing strip 622 and the second sealing strip 624, and can increase the contact area between the first sealing strip 622 and the sealing strip 622. The contact area between the two bars 624 further ensures the sealing performance of the joint between the sealed door panel 615 and the port 4, preventing heat from leaking through the joint between the sealed door panel 615 and the port 4;

When it is necessary to take out the culture bottle from the cell culture device body 1, the staff takes out the culture bottle from the placement cylinder 5 through the material taking mechanism 7, and the material taking mechanism 7 first controls the three jaws 83 to move to the right above the culture bottle that needs to be taken out Specifically, when the first driving motor 713 is running, it can drive the first sliding seat 712 to move on the first fixed seat 711, the second sliding seat 723 can move on the second fixed seat 721, and the second driving motor 722 can drive The lifting slider 731 moves up and down, so, under the cooperative action of the first moving assembly 71, the second moving assembly 72 and the third moving assembly 73, the feeding mechanism 7 controls the three jaws 83 to move directly above the culture bottle, and then the lifting The slider 731 drives the three jaws 83 to move downward until the three jaws 83 move to the mouth of the culture bottle, then the servo motor 86 runs, and the servo motor 86 can drive the worm 87 to rotate when the servo motor 86 is running. During the rotation process, the three worm gears 85 can be driven to rotate synchronously, and the three worm gears 85 can drive the three shaft rods 84 to rotate during the rotation process, thereby causing the three jaws 83 to rotate, so that the three jaws 83 Close to each other until the three jaws 83 are in contact with the mouth of the culture bottle, so that the clamping assembly 8 can use the three jaws 83 to clamp the culture bottle, and further, the second driving motor 722 drives the lifting slider 731 moves upwards, so that the clamping assembly 8 drives the culture bottle to move upwards, and then, the first moving assembly 71, the second moving assembly 72 and the third moving assembly 73 drive the culture bottle to move to the position facing the port 4;

Afterwards, cylinder one 612 runs, and cylinder one 612 can drive lifting seat 613 to move upwards during operation, and lifting seat 613 can drive sealing door panel 615 to move upwards when moving up, until sealing door panel 615 no longer blocks port 4, and then, cylinder two 733 Running, the cylinder 2 733 can drive the clamping assembly 8 to move when it is running, until the culture bottle moves to the feeding area in the cell culture device body 1 through the port 4, and the culture bottle moves to the placement cylinder in the feeding area 5, finally, the second drive motor 722 drives the lifting slider 731 to move downward until the culture bottle moves to the inside of the placement cylinder 5;

When no culture bottle is placed in the placement cylinder 5, under the action of the two airbags 923, the two clamping plates 915 are in a state of being far away from each other. When the culture bottle falls into the placement cylinder 5, the culture bottle will fall on the sliding plate 911, the sliding plate 911 will move downward under the gravity of the culture bottle. When the sliding plate 911 moves downward, it can squeeze the air bag one 921. When the air bag one 921 is squeezed, it can press the internal gas through the two air tubes into the two airbags 923, so that the two airbags 923 are inflated, and when the two airbags 923 are inflated, the two clamping plates 915 can be driven close to each other, so that the two clamping plates 915 can clamp the culture bottle , the culture bottle can be fixed in the placement tube 5 to ensure the stability of the culture bottle in the placement tube 5;

When the culture bottle that needs to be taken out falls into the inside of the placement cylinder 5 in the fetching area, the moving assembly 1 71, the moving assembly 2 72 and the moving assembly 3 73 drive the clamping assembly 8 to move to the culture area in the cell culture device body 1 again , cylinder one 612 drives the sealing door 615 to move downwards, so that the sealing door 615 completely blocks the port 4, and finally, the staff opens the box door 2, and takes out the culture bottle from the placement cylinder 5 in the picking area, based on the above operations , under the cooperative action of the separation mechanism and the retrieving mechanism, the clamping assembly 8 can place the culture bottle that needs to be taken out into the placement cylinder 5 in the retrieving area, and then the sealing door plate 615 can completely cover the port 4, and can Avoiding the temperature loss of the culture area in the cell culture device body 1 to the outside, thereby avoiding large fluctuations in the temperature of the culture area in the cell culture device body 1, ensuring the constant temperature effect of the culture area in the cell culture device body 1, thereby ensuring that the cell culture device body 1 for the effect of cell culture.

It should be noted that the specific structures and working principles of the first mobile unit 71 , the second mobile unit 72 and the third mobile unit 73 are not considered as innovative parts of the technical solution, are not shown in the figure, and will not be described in detail here.

The above is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto, any person familiar with the technical field within the technical scope disclosed in the present invention, according to the technical solution of the present invention Any equivalent replacement or change of the inventive concepts thereof shall fall within the protection scope of the present invention.

Claims (10)

1. The cell culture device for biological analysis comprises a cell culture device body (1), wherein a box door (2) is rotatably assembled on the cell culture device body (1), and the cell culture device is characterized in that the inside of the cell culture device body (1) is divided into a culture area and a material taking area through a partition plate (3), a through hole (4) is formed in the bottom end position of the partition plate (3), a plurality of placing cylinders (5) are assembled in the cell culture device body (1), one of the placing cylinders (5) is positioned in the material taking area, and the rest of the placing cylinders (5) are positioned in the culture area;

a separation mechanism (6) is arranged on the separation plate (3), and the separation mechanism (6) comprises a lifting assembly (61) and a sealing assembly (62);

a material taking mechanism (7) is arranged in the cell culture device body (1), and the material taking mechanism (7) comprises a first moving component (71), a second moving component (72) and a third moving component (73);

the inside of the cell culture device body (1) is also provided with a clamping component (8);

a plurality of placing cylinders (5) are respectively provided with a fixing mechanism (9), and the fixing mechanisms (9) comprise clamping assemblies (91) and triggering assemblies (92).

2. The cell culture apparatus for biological analysis according to claim 1, wherein the lifting assembly (61) comprises a fixing frame (611), a first cylinder (612), a lifting seat (613), a guide sliding rail (614) and a sealing door plate (615), the fixing frame (611) is fixedly connected to the partition plate (3), the first cylinder (612) is mounted on the fixing frame (611), the lifting seat (613) is fixedly connected to a telescopic end of the first cylinder (612), the guide sliding rail (614) is fixedly connected to the partition plate (3), the lifting seat (613) is slidably assembled on the guide sliding rail (614), the sealing door plate (615) is fixedly connected to the lifting seat (613), and the sealing door plate (615) is slidably assembled in the through hole (4).

3. The cell culture device for biological analysis according to claim 1, wherein the sealing component (72) comprises four sealing sheets (621), a first sealing strip (622), a plurality of grooves (623), a second sealing strip (624) and a plurality of protruding blocks (625), the four sealing sheets (621) are in pairs and are respectively and fixedly connected to two opposite inner surfaces of the through hole (4), the four sealing sheets (621) are respectively positioned on two sides of the sealing door plate (615), the four sealing sheets (621) are mutually attached to the sealing door plate (615), the first sealing strip (622) is fixedly connected to the bottom surface of the sealing door plate (615), the grooves (623) are respectively formed on the first sealing strip (622), the second sealing strip (624) is fixedly connected to the inner bottom surface of the through hole (4), the protruding blocks (625) are respectively and directly opposite to the grooves (623).

4. The cell culture apparatus according to claim 1, wherein the first moving assembly (71) comprises a first fixing base (711), a first sliding base (712) and a first driving motor (713), the first fixing base (711) is fixedly connected to the inner surface of the cell culture apparatus body (1), the first sliding base (712) is slidably assembled on the first fixing base (711), the first driving motor (713) is mounted on the first sliding base (712), and the first driving motor (713) is used for driving the first sliding base (712) to move on the first fixing base (711).

5. The cell culture apparatus according to claim 4, wherein the second moving assembly (72) comprises a second fixed base (721), a second driving motor (722) and a second sliding base (723), the second fixed base (721) is fixedly connected to the first sliding base (712), the second sliding base (723) is slidably assembled on the second fixed base (721), and the second driving motor (722) is mounted on the second sliding base (723).

6. The cell culture apparatus according to claim 5, wherein the third moving unit (73) comprises a lifting slider (731), a mounting frame (732) and a second cylinder (733), the lifting slider (731) is slidably mounted on the second sliding seat (723), the mounting frame (732) is fixedly connected to the lifting slider (731), and the second cylinder (733) is mounted on the mounting frame (732).

7. The cell culture apparatus for biological analysis according to claim 6, wherein the clamping assembly (8) comprises a clamping seat (81), a plurality of fixing plates (82), three clamping jaws (83), three shaft rods (84), three worm gears (85), a servo motor (86) and a worm (87), the clamping seat (81) is fixedly connected to the telescopic end of a cylinder two (733), a plurality of fixing plates (82) are fixedly connected to the clamping seat (81), the three shaft rods (84) are rotatably connected between the fixing plates (82) opposite to each other, the three clamping jaws (83) are fixedly sleeved on the three shaft rods (84) respectively, the three worm gears (85) are fixedly sleeved on the three shaft rods (84) respectively, the servo motor (86) is mounted on the clamping seat (81), the worm (87) is fixedly connected to the telescopic end of the servo motor (86), and the worm (87) and the worm gears (85) are meshed with each other.

8. The cell culture apparatus for biological analysis according to claim 1, wherein the clamping assembly (91) comprises a sliding plate (911), a base (912), a limiting groove (913), two grooves (914), two clamping plates (915), four sliding grooves (916) and four sliding blocks (917), the sliding plate (911) is slidably assembled on the inner surface of the placement barrel (5), the base (912) is fixedly connected to the top surface of the sliding plate (911), the limiting groove (913) is formed in the base (912), two grooves (914) are formed in the inner surface of the placement barrel (5), two clamping plates (915) are slidably connected in the two grooves (914), four sliding grooves (916) are formed in a group of two grooves (914), four sliding blocks (917) are formed in a group of two grooves (915), are fixedly connected to the two clamping plates (915), and the four sliding blocks (917) are slidably assembled in the four sliding grooves (916).

9. The cell culture apparatus according to claim 8, wherein the trigger unit (92) comprises a first air bag (921), a first spring (922), two second air bags (923) and two second springs (924), the first air bag (921) is arranged between the inner bottom surface of the placement cylinder (5) and the sliding plate (911), the two second air bags (923) are respectively arranged between the two grooves (914) and the two clamping plates (915), the first spring (922) is assembled inside the first air bag (921), the two second springs (924) are respectively assembled inside the two second air bags (923), and the two second air bags (923) are communicated with the first air bag (921) through air pipes.

10. The cell culture apparatus for bioanalytical applications according to claim 7, wherein the three clamping jaws (83) are each bonded with a non-slip pad.

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