CN116391129A - 针对ras蛋白的靶标接合测定法 - Google Patents

针对ras蛋白的靶标接合测定法 Download PDF

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Publication number
CN116391129A
CN116391129A CN202180074299.3A CN202180074299A CN116391129A CN 116391129 A CN116391129 A CN 116391129A CN 202180074299 A CN202180074299 A CN 202180074299A CN 116391129 A CN116391129 A CN 116391129A
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Prior art keywords
ras
protein
kras
methyl
functional element
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Pending
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CN202180074299.3A
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English (en)
Chinese (zh)
Inventor
M·B·罗伯斯
J·R·沃克
J·瓦斯塔
C·科罗纳
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Promega Corp
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Promega Corp
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Publication of CN116391129A publication Critical patent/CN116391129A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/02—Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B (e.g. A = enzyme and B = substrate for A, or A = receptor and B = ligand for the receptor)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/20—Screening for compounds of potential therapeutic value cell-free systems

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)
  • Peptides Or Proteins (AREA)
CN202180074299.3A 2020-08-28 2021-08-27 针对ras蛋白的靶标接合测定法 Pending CN116391129A (zh)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US202063071694P 2020-08-28 2020-08-28
US63/071694 2020-08-28
US202063117080P 2020-11-23 2020-11-23
US63/117080 2020-11-23
US202163160120P 2021-03-12 2021-03-12
US63/160120 2021-03-12
PCT/US2021/047998 WO2022047186A1 (en) 2020-08-28 2021-08-27 Target engagement assay for ras proteins

Publications (1)

Publication Number Publication Date
CN116391129A true CN116391129A (zh) 2023-07-04

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ID=77914456

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202180074299.3A Pending CN116391129A (zh) 2020-08-28 2021-08-27 针对ras蛋白的靶标接合测定法

Country Status (5)

Country Link
US (1) US20220064696A1 (https=)
EP (1) EP4204808A1 (https=)
JP (1) JP2023540934A (https=)
CN (1) CN116391129A (https=)
WO (1) WO2022047186A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20210006356A (ko) 2018-04-04 2021-01-18 아비나스 오퍼레이션스, 인코포레이티드 단백질분해 조절제 및 연관된 사용 방법
CN121419983A (zh) 2023-01-26 2026-01-27 阿尔维纳斯运营股份有限公司 基于小脑蛋白的kras降解protac及其相关用途

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170131278A1 (en) * 2015-10-19 2017-05-11 Araxes Pharma Llc Method for screening inhibitors of ras

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4812409A (en) 1986-01-31 1989-03-14 Eastman Kodak Company Hydrolyzable fluorescent substrates and analytical determinations using same
US4810636A (en) 1986-12-09 1989-03-07 Miles Inc. Chromogenic acridinone enzyme substrates
US6162931A (en) 1996-04-12 2000-12-19 Molecular Probes, Inc. Fluorinated xanthene derivatives
US7429472B2 (en) 2003-01-31 2008-09-30 Promega Corporation Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule
EP1594962B1 (en) 2003-01-31 2014-08-27 Promega Corporation Covalent tethering of functional groups to proteins
US7425436B2 (en) 2004-07-30 2008-09-16 Promega Corporation Covalent tethering of functional groups to proteins and substrates therefor
WO2008118445A1 (en) 2007-03-26 2008-10-02 Promega Corporation Methods to quench light from optical reactions
GB0905840D0 (en) 2009-04-06 2009-05-20 Sagentia Ltd Apparatus and methods
ES2795287T3 (es) 2009-05-01 2020-11-23 Promega Corp Luciferasas de Oplophorus sintéticas con mayor emisión de luz
JP5677041B2 (ja) 2009-11-11 2015-02-25 株式会社ニデック 眼科装置
SG10202103336SA (en) 2010-11-02 2021-04-29 Promega Corp Novel coelenterazine substrates and methods of use
GB201103631D0 (en) * 2011-03-03 2011-04-13 Univ Leeds Identification of candidate therapeutics
JP5751030B2 (ja) 2011-06-03 2015-07-22 ソニー株式会社 表示制御装置、表示制御方法、及びプログラム
WO2013078244A1 (en) 2011-11-21 2013-05-30 Promega Corporation Carboxy x rhodamine analogs
US20140099654A1 (en) 2012-09-26 2014-04-10 Promega Corporation Real-time monitoring
KR102292431B1 (ko) 2012-12-12 2021-08-20 프로메가 코포레이션 세포내 생물발광 공명 에너지 전이를 이용하여 생물활성제에 의한 세포 표적 결합의 인식
US10067149B2 (en) * 2012-12-12 2018-09-04 Promega Corporation Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer
ES2926463T3 (es) 2013-03-15 2022-10-26 Promega Corp Activación de la bioluminiscencia mediante complementación estructural
JP7092677B2 (ja) 2016-02-15 2022-06-28 プロメガ コーポレイション セレンテラジン類似体
JP7212612B2 (ja) 2016-07-28 2023-01-25 プロメガ コーポレイション セレンテラジン類縁体
WO2019173683A1 (en) * 2018-03-08 2019-09-12 Stc. Unm Discovery of novel molecules and repurposed drugs for ras family gtpases
WO2019241438A2 (en) 2018-06-12 2019-12-19 Promega Corporation Multipartite luciferase

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170131278A1 (en) * 2015-10-19 2017-05-11 Araxes Pharma Llc Method for screening inhibitors of ras

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ANDREW S. DIXON等: "NanoLuc Complementation Reporter Optimized for Accurate Measurement of Protein Interactions in Cells.", 《ACS CHEM. BIOL.》, 16 November 2015 (2015-11-16), pages 403 *
DIRK KESSLER等: "Drugging an undruggable pocket on KRAS.", 《PNAS》, vol. 116, no. 32, 6 August 2019 (2019-08-06), pages 4, XP055671320, DOI: 10.1073/pnas.1904529116 *
JAMES D. VASTA等: "Quantitative, Wide-Spectrum Kinase Profiling in Live Cells for Assessing the Effect of Cellular ATP on Target Engagement.", 《CELL CHEMICAL BIOLOGY》, 15 February 2018 (2018-02-15), pages 207 *

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US20220064696A1 (en) 2022-03-03
EP4204808A1 (en) 2023-07-05
WO2022047186A1 (en) 2022-03-03
JP2023540934A (ja) 2023-09-27

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