CN116376672A - A kind of production device of vitamin B2 - Google Patents

Abstract

The invention relates to the technical field of fermentation tanks, and discloses a vitamin B2 production device, which comprises a fermentation tank, wherein the fermentation tank is provided with a fermentation tank body; a stirring assembly and an air inlet assembly are arranged in the fermentation tank; the stirring assembly comprises a driving motor A, a hollow rotating shaft A, a plurality of stirring rods B and side wall scraping plates; the air inlet assembly comprises a driving motor B, a hollow rotating shaft B, two air distribution pipes, a screw conveying blade and a bottom scraping plate; the side wall scraping plate and the nozzle are arranged on the stirring assembly, so that the inner side wall of the lower tank body is convenient to clean; the air inlet component not only ventilates the fermentation tank in the fermentation process, but also can ventilate high-temperature steam or ozone into the fermentation tank to sterilize the fermentation tank in the cleaning process.

Description

A kind of production device of vitamin B2

technical field

The invention belongs to the technical field of fermentation tanks, in particular to a vitamin B2 production device.

Background technique

Vitamin B2, also known as riboflavin, is one of the B vitamins, slightly soluble in water, and stable when heated in neutral or acidic solutions. It is a part of the prosthetic group of yellow enzymes in the body. If it is lacking, it will affect the biological oxidation of the body and cause metabolic disorders.

The process of producing vitamin B2 in the prior art is as follows: put the fermentation medium into the fermenter, and add the sterilized starch glucose solution at one time under the condition of introducing sterile clean air; after disinfection, move into the cultured seed solution , start to ferment. After fermentation for a period of time, measure the amount of residual sugar until the glucose in the tank is exhausted, then start the mechanical stirring after maintaining for a period of time, and add the sterilized starch glucose solution at a certain flow rate; Keep the fermentation temperature at 37-39°C, and control the ventilation to maintain the dissolved oxygen in the fermenter above 20%. From the start of fed-batch feeding, measure the riboflavin titer every 8 hours until the given glucose is consumed in 38-42 hours or When the dissolved oxygen content rises to 60%, the fermentation is stopped, and the fermented liquid enters the extraction process to extract riboflavin. Therefore, in the fermentation process, it is necessary to continuously feed gas and feed sterilized starch glucose solution, which means that it is necessary to continuously replenish materials and discharge gas in the fermenter; Sterilized starch glucose solution, direct addition of sterilized starch glucose solution will affect the temperature of the material in the fermentation tank and affect the fermentation. And in the process of exhausting, the exhaust pipe is directly connected with the external air, and there will be impurities or bacteria entering the fermenter from the exhaust pipe, which will affect the fermentation situation. In addition, in order to ensure the smooth progress of fermentation, the fermenter needs to be cleaned and sterilized before fermentation to avoid the generation of bacteria in the tube; however, it is inconvenient to clean the fermenter in the prior art.

Therefore, there is an urgent need for a vitamin B2 production device to solve the above technical problems.

Contents of the invention

The purpose of the present invention is to overcome the existing technical problems and provide a vitamin B2 production device.

To achieve the above object, the present invention is implemented according to the following technical solutions:

A production device for vitamin B2, comprising a fermenter; the fermenter includes a flange-connected upper cover and a lower tank, the lower tank is provided with a water circulation jacket; the upper end of the upper cover is provided with a feed pipe , exhaust pipe, the bottom of the lower tank is provided with a discharge pipe; the fermentation tank is provided with a stirring assembly and an air intake assembly;

The stirring assembly includes a driving motor A, a hollow rotating shaft A, a plurality of stirring rods A, a plurality of stirring rods B, and a side wall scraper; the hollow rotating shaft A runs through the upper cover and is rotatably connected with the upper cover; a plurality of stirring rods A is fixedly arranged on the left side of the hollow shaft A from top to bottom, and multiple stirring rods B are fixedly arranged on the right side of the hollow shaft A from top to bottom; the stirring rods A are connected to the side wall scrapers through elastic components , the end of the side wall scraper is in contact with the inner side wall of the lower tank; the stirring rod B is a hollow structure, connected with the hollow shaft A, and the end of the stirring rod B is equipped with a nozzle; the head of the hollow shaft A is connected with a rotary joint , the tail is closed; the drive motor A drives the hollow rotating shaft A to rotate; the air intake assembly is used to feed gas into the fermenter.

Preferably, the discharge pipe is L-shaped, including vertical pipes and horizontal pipes;

The air intake assembly includes a drive motor B, a hollow shaft B, two air distribution pipes, a screw conveyor blade, and a bottom scraper; the hollow shaft B runs through the bottom of the vertical pipe and is rotationally connected with the vertical pipe; the two air distribution pipes are left and right symmetrical Set on the hollow rotating shaft B, the air distribution pipe is evenly distributed with air distribution holes; the lower end of the air distribution pipe is connected with a bottom scraper through a connecting rod, and the bottom scraper contacts the bottom of the lower tank body; the screw conveying blade is installed in the vertical pipe on the hollow shaft B; the head of the hollow shaft B is closed, and a rotary joint is installed at the tail; the driving motor B drives the hollow shaft B to rotate.

The drive motor and rotary joint in the present invention can be selected from those commonly used in the field, and those skilled in the art can select and control them according to actual usage requirements. When the present invention is in use, aseptic clean air is introduced into the hollow rotating shaft B, and the air distribution pipe on the hollow rotating shaft B passes the sterile clean air into the fermenter from the bottom, and the spiral conveying paddles store up the air in the vertical pipe The materials in the fermenter are constantly stirred up, and the scraper at the bottom can turn the materials accumulated at the bottom, so that the materials in the fermentation tank are mixed more evenly. After the fermentation is completed, the cleaning liquid (clean water or other liquid for cleaning the tank body) can be passed into the fermenter through the hollow shaft A, and the nozzle on the stirring rod B sprays the cleaning liquid down the side wall of the tank body, and the side wall is scraped The plate cleans the side wall, and the bottom scraper cleans the bottom to clean the lower tank. Subsequently, high-temperature steam or ozone can be introduced into the fermenter through the hollow rotating shaft B to sterilize the interior of the fermenter.

Preferably, an elastic assembly is provided on the left side of each stirring rod A; the elastic assembly includes a mounting plate, a sliding rod, a spring, and a baffle; the mounting plate is fixedly connected to the stirring rod A, and the left end of the sliding rod and the side The wall scraper is fixedly connected, and the right end of the sliding rod passes through the mounting plate and is fixedly connected with the baffle; the sliding rod between the mounting plate and the side wall scraper is sleeved with a spring.

In the arrangement of the elastic component, the spring gives the side wall scraper an elastic force directed horizontally to the side wall of the lower tank body, so that the side wall scraper can be in close contact with the inner side wall of the lower tank body.

Preferably, the lower tank body is provided with a feeding ring pipe, and the lower end surface of the feeding ring pipe is evenly distributed with feeding holes; the feeding ring pipe is located above the stirring rod A; the left side of the lower tank body is connected with a feeding pipe, and the feeding pipe runs through the lower tank body The side wall of the tank is connected with the feeding ring pipe; the feeding pipe outside the lower tank is provided with a valve A, the feeding pipe on the right side of the valve A is connected with a cleaning branch pipe, and the cleaning branch pipe is provided with a valve B.

The feed pipe is used to add sterilized starch glucose solution into the fermenter, and pass into the fermenter through the feeding ring pipe to make the cloth more uniform. The setting of the cleaning branch pipe is convenient to pass cleaning liquid, or high-temperature steam or ozone into the feeding pipe and the feeding ring pipe through the cleaning branch pipe to clean and sterilize the feeding pipe and the feeding ring pipe.

Preferably, the production device further includes a heating tank; a rotatable U-shaped stirring paddle is arranged in the heating tank, a liquid feeding pipe and a liquid suction pipe are connected to the upper surface of the heating tank, and a liquid discharge pipe is connected to the bottom of the heating tank. tube; the outside of the heating tank is provided with a heating jacket, and the inner wall of the heating tank is equidistantly distributed with a number of ultraviolet sterilizing lamps A; the upper surface of the heating tank is provided with a number of ultraviolet sterilizing lamps B; Stretch into the heating tank; the pumping pipe communicates with the feeding pipe through the pipeline.

The heating tank is used to heat and sterilize the starch glucose solution when in use, so that the temperature of the sterilized starch glucose solution is consistent with the temperature in the fermenter, avoiding the influence of the temperature in the fermenter during the process of adding the sterilized starch glucose solution, and maintaining the fermentation stability. The use of ultraviolet sterilizing lamps facilitates the continuous sterilization of the sterilized starch glucose solution in the heating tank to avoid contamination during the heating process.

Preferably, the tail end of the liquid suction pipe extends into the heating tank, and is detachably connected with a transparent connecting pipe; the tail of the transparent connecting pipe is close to the bottom of the heating tank.

The transparent connecting tube is made of a transparent material through which ultraviolet rays can pass, and those skilled in the art can choose it according to the actual use effect, specifically, a quartz tube can be selected. The use of the transparent connecting tube can make the sterilized starch glucose solution also be sterilized by ultraviolet irradiation in the transparent tube.

Preferably, the exhaust pipe is also communicated with an exhaust assembly;

The exhaust assembly shown includes an exhaust box, and the upper end of the exhaust box is connected with an exhaust inlet pipe and an exhaust outlet pipe; a sealing liquid is also stored in the exhaust box; the tail end of the exhaust inlet pipe extends into the sealing liquid Among them, the tail end of the exhaust outlet pipe is far away from the upper end surface of the sealing liquid, and the exhaust pipe is connected with the exhaust inlet pipe.

The sealing liquid should be pure water, or a solution with sterilizing effect. The exhaust box is also provided with a sealing liquid inlet pipe which is convenient for the sealing liquid to enter, and a sealing liquid discharge pipe which is convenient for the sealing liquid to discharge. When in use, the gas discharged from the fermenter enters the exhaust inlet pipe from the exhaust pipe, and the gas discharged from the exhaust inlet pipe enters the sealing liquid, then is discharged from the sealing liquid, and is discharged from the exhaust outlet pipe. The arrangement of the exhaust assembly ensures that the gas discharged from the fermenter can be discharged smoothly without gas entering the fermenter from the exhaust pipe.

Preferably, a buffer ball is detachably connected between the exhaust pipe and the exhaust inlet pipe.

The setting of the buffer ball can make the sealing liquid directly suck back into the buffer ball when the exhaust pipe sucks back, instead of entering the fermenter along the exhaust pipe. The buffer ball is made of transparent material, which is convenient to observe the condition of the buffer ball, so as to replace the new buffer ball in time.

The unrestricted components in the present invention all adopt conventional means in this field, and those skilled in the art can select their models and installation methods according to actual usage requirements, and clearly understand how to install and control them specifically, and will not repeat them here. A detailed description.

The present invention has reached following beneficial effect:

The present invention is simple in structure and easy to operate; the mixing assembly of the present invention is provided with a side wall scraper and a nozzle to facilitate the cleaning of the inner side wall of the lower tank body; the air intake assembly not only ventilates the fermentation tank during the fermentation process, but also During the cleaning process, high-temperature steam or ozone can be introduced into the fermenter to sterilize the fermenter; the setting of the heating tank is convenient to feed the sterilized starch glucose solution that is consistent with the temperature of the material in the fermenter to reduce the flow rate. The effect of adding materials on the internal temperature of the fermenter; the exhaust assembly is set so that during the exhaust process, it can only be exhausted from the exhaust pipe, and there will be no external gas carrying bacteria into the exhaust pipe.

Description of drawings

Fig. 1 is the structural representation of embodiment 1 of the present invention;

Figure 2 is an enlarged view at E in Figure 1;

Fig. 3 is the structural representation of embodiment 2 of the present invention;

Fig. 4 is the structural representation of heating tank in Fig. 3;

FIG. 5 is a schematic structural view of Embodiment 3 of the present invention;

Fig. 6 is a structural schematic diagram of the exhaust assembly and the buffer ball in Fig. 5 .

In the figure: 1. Upper cover body; 2. Lower tank body; 3. Water circulation jacket; 4. Feed pipe; 5. Exhaust pipe; 6. Discharge pipe; 7. Drive motor A; 8. Hollow shaft A ;9, stirring rod A; 10, stirring rod B; 11, side wall scraper; 12, nozzle; 13, rotary joint; 14, vertical pipe; 15, drive motor B; 16, hollow shaft B; 17, spiral Conveying paddle; 18. Bottom scraper; 19. Air distribution pipe; 20. Mounting plate; 21. Slide rod; 22. Spring; 23. Block; 24. Feeding ring pipe; 25. Feeding hole; 27. Valve A; 28. Cleaning branch pipe; 29. Valve B; 30. Heating tank; 31. U-shaped stirring paddle; 32. Adding pipe; 33. Pumping pipe; 34. Draining pipe; 35. Heating jacket ; 36, ultraviolet sterilizing lamp A; 37, ultraviolet sterilizing lamp B; 38, transparent connecting pipe; 39, exhaust box; 40, exhaust inlet pipe; 41, exhaust outlet pipe; 42, sealing liquid; 43, Buffer ball; 44, sealing liquid inlet pipe; 45, sealing liquid discharge pipe; 46, inlet; 47, mouth.

Detailed ways

The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments. The schematic embodiments and descriptions of the present invention are used to explain the present invention, but are not intended to limit the present invention.

Example 1

As shown in Figures 1 to 2, a vitamin B2 production device includes a fermenter; the fermenter includes a flange-connected upper cover 1 and a lower tank 2, and the lower tank 2 is externally provided with a water circulation jacket 3. The upper end surface of the upper cover body 1 is provided with a feed pipe 4 and an exhaust pipe 5, and the bottom end of the lower tank body 2 is provided with a discharge pipe 6; the fermentation tank is provided with a stirring assembly and an air intake assembly;

The stirring assembly includes a driving motor A7, a hollow rotating shaft A8, three stirring rods A9, three stirring rods B10, and a side wall scraper 11; the hollow rotating shaft A8 runs through the upper cover 1 and is rotationally connected with the upper cover 1; One stirring rod A9 is fixedly arranged on the left side of the hollow rotating shaft A8 equidistantly from top to bottom, and three stirring rods B10 are fixedly arranged equidistantly on the right side of the hollow rotating shaft A8 from top to bottom; The scraper 11 is connected, and the end of the side wall scraper 11 is in contact with the inner side wall of the lower tank body 2; the stirring rod B10 is a hollow structure, communicated with the hollow shaft A8, and the end of the stirring rod B10 is equipped with a nozzle 12; the hollow shaft A8 The head is connected with a rotary joint 13, and the tail is closed; the drive motor A7 drives the hollow shaft A8 to rotate;

The air intake assembly is used for introducing gas into the fermenter.

The discharge pipe is L-shaped, including vertical pipe 14 and horizontal pipe;

The intake assembly includes a drive motor B15, a hollow shaft B16, two air distribution pipes 19, a screw conveying paddle 17, and a bottom scraper 18; the hollow shaft B16 runs through the bottom of the vertical pipe 14 and is rotationally connected with the vertical pipe 14; two The air distribution pipe 19 is symmetrically arranged on the hollow rotating shaft B16, and the air distribution pipe 19 is evenly distributed with air distribution holes; the lower end of the air distribution pipe 19 is connected with a bottom scraper 18 through a connecting rod, and the bottom scraper 18 is in contact with the bottom of the lower tank body 2 The screw conveying paddle 17 is installed on the hollow shaft B located in the vertical pipe 14; the head of the hollow shaft B16 is closed, and the tail is equipped with a rotary joint; the drive motor B15 drives the hollow shaft B16 to rotate.

The left side bottom of each stirring rod A9 is all provided with an elastic assembly; Said elastic assembly comprises mounting plate 20, slide bar 21, spring 22, catch plate 23; Mounting plate 20 is fixedly connected with stirring rod A9, and the sliding rod 21 The left end is fixedly connected with the side wall scraper 11, and the right end of the sliding rod 21 passes through the mounting plate 20 and is fixedly connected with the blocking piece 23;

The lower tank body is provided with a feeding ring pipe 24, and the lower end surface of the feeding ring pipe 24 is evenly distributed with feeding holes 25; the feeding ring pipe 24 is located above the uppermost stirring rod A and stirring rod B; the left side of the lower tank body 2 is connected with a feeding Pipe 26, the feed pipe 26 runs through the side wall of the lower tank body 2 and communicates with the feed ring pipe 24; the feed pipe outside the lower tank body 2 is provided with a valve A27, and the feed pipe on the right side of the valve A27 is connected with a cleaning branch pipe 28 , The cleaning branch pipe 28 is provided with a valve B29.

When the present embodiment is in use, bacteria species, culture medium and other raw materials are dropped into the fermenter from the feed pipe. During the fermentation process, the sterile clean air is fed through the air intake assembly, and the sterilized starch glucose solution is fed into the fermenter through the feeding pipe and the feeding ring pipe. After the fermentation is completed, the fermentation tank is cleaned, sterilized and sterilized by using the stirring component and the air intake component.

Example 2

As shown in Fig. 3 and Fig. 4, on the basis of embodiment 1, the described production device also includes a heating tank 30; A liquid feeding pipe 32 and a liquid pumping pipe 33 are connected, and a liquid discharge pipe 34 is connected to the bottom of the heating tank 30; a heating jacket 35 is arranged on the outside of the heating tank 30, and a number of ultraviolet rays are equidistantly distributed on the inner wall of the heating tank 30. The sterilizing lamp A36; the upper end surface of the heating tank 30 is provided with several ultraviolet sterilizing lamps B37; the ultraviolet sterilizing lamp B37 extends into the heating tank 30;

The tail end of the suction pipe 33 extends into the heating tank 30, and is detachably connected with a transparent connecting pipe 38; the tail of the transparent connecting pipe 38 is close to the bottom of the heating tank.

When the present embodiment is in use, the sterilized starch glucose solution can be passed into the heating tank for stirring and heating, and the ultraviolet sterilizing lamp can be used continuously for sterilization. Then the sterilized starch glucose solution whose temperature is consistent with the temperature in the fermentation tank is passed into the fermentation tank through the feed pipe and the feed ring pipe.

Example 3

As shown in Figures 5 and 6, on the basis of Embodiment 2, the exhaust pipe 5 is also connected with an exhaust assembly;

The exhaust assembly shown includes an exhaust box 39, and the upper surface of the exhaust box 39 is connected with an exhaust inlet pipe 40 and an exhaust outlet pipe 41; a sealing liquid 42 is also stored in the exhaust box 39; the exhaust inlet pipe 40 The tail end extends into the sealing liquid 42 , the tail end of the exhaust outlet pipe 41 is away from the upper surface of the sealing liquid 42 , and the exhaust pipe 5 communicates with the exhaust inlet pipe 40 .

A buffer ball 43 is detachably connected between the exhaust pipe 5 and the exhaust inlet pipe 40 . The exhaust box is also provided with a sealing liquid inlet pipe 44 which is convenient for the sealing liquid to enter, and a sealing liquid discharge pipe 45 which is convenient for the sealing liquid to discharge.

The sealing fluid in this embodiment can be pure water.

When this embodiment is in use, the exhaust pipe is detachably connected to the buffer ball through the pipe, and the buffer ball is detachably connected to the exhaust inlet pipe through the pipe. The inlet 46 of the buffer ball is located at the leftmost side of the buffer ball, and the outlet 47 of the buffer ball is located at the rightmost side of the buffer ball. After using for a period of time, the pipeline, buffer ball and exhaust assembly can be disassembled for cleaning and sterilization.

The technical solution of the present invention is not limited to the limitations of the above-mentioned specific embodiments, and any technical deformation made according to the technical solution of the present invention falls within the protection scope of the present invention.

Claims (8)

1. A production device for vitamin B2, comprising a fermenter; said fermenter comprises a flanged upper cover and a lower tank, the lower tank is provided with a water circulation jacket outside; the upper end of the upper cover is provided with an inlet The material pipe, the exhaust pipe, and the bottom of the lower tank are provided with a discharge pipe; the fermentation tank is provided with a stirring assembly and an air intake assembly; it is characterized in that: The stirring assembly includes a driving motor A, a hollow rotating shaft A, a plurality of stirring rods A, a plurality of stirring rods B, and a side wall scraper; the hollow rotating shaft A runs through the upper cover and is rotatably connected with the upper cover; a plurality of stirring rods A is fixedly arranged on the left side of the hollow shaft A from top to bottom, and multiple stirring rods B are fixedly arranged on the right side of the hollow shaft A from top to bottom; the stirring rods A are connected to the side wall scrapers through elastic components , the end of the side wall scraper is in contact with the inner side wall of the lower tank; the stirring rod B is a hollow structure, connected with the hollow shaft A, and the end of the stirring rod B is equipped with a nozzle; the head of the hollow shaft A is connected with a rotary joint , the tail is closed; the drive motor A drives the hollow shaft A to rotate; The air intake assembly is used for introducing gas into the fermenter. 2. The production device of a kind of vitamin B2 according to claim 1, characterized in that: the discharge pipe is L-shaped, including vertical pipes and horizontal pipes; The air intake assembly includes a drive motor B, a hollow shaft B, two air distribution pipes, a screw conveyor blade, and a bottom scraper; the hollow shaft B runs through the bottom of the vertical pipe and is rotationally connected with the vertical pipe; the two air distribution pipes are left and right symmetrical Set on the hollow rotating shaft B, the air distribution pipe is evenly distributed with air distribution holes; the lower end of the air distribution pipe is connected with a bottom scraper through a connecting rod, and the bottom scraper contacts the bottom of the lower tank body; the screw conveying blade is installed in the vertical pipe on the hollow shaft B; the head of the hollow shaft B is closed, and a rotary joint is installed at the tail; the driving motor B drives the hollow shaft B to rotate. 3. The production device of a kind of vitamin B2 according to claim 2, characterized in that: an elastic assembly is provided on the left side of each stirring rod A; The baffle; the mounting plate is fixedly connected with the stirring rod A, the left end of the sliding rod is fixedly connected with the side wall scraper, and the right end of the sliding rod passes through the mounting plate and is fixedly connected with the baffle; the sliding between the mounting plate and the side wall scraper The rod is sheathed with a spring. 4. the production device of a kind of vitamin B2 according to claim 3 is characterized in that: the lower tank body is provided with feeding ring pipe, and the lower end face of feeding ring pipe is evenly distributed with feeding holes; Above; the left side of the lower tank body is connected with a feeding pipe, which runs through the side wall of the lower tank body and communicates with the feeding ring pipe; the feeding pipe outside the lower tank body is provided with a valve A, and the feeding pipe on the right side of the valve A is It is connected with a cleaning branch pipe, and a valve B is arranged on the cleaning branch pipe. 5. The production device of a kind of vitamin B2 according to claim 4, characterized in that: the production device also includes a heating tank; a rotatable U-shaped stirring paddle is arranged in the heating tank, and the upper end surface of the heating tank There are liquid feeding pipes and pumping pipes connected, and the bottom of the heating tank is connected with a liquid discharge pipe; the outside of the heating tank is provided with a heating jacket, and a number of ultraviolet sterilizing lamps A are distributed equidistantly around the inner wall of the heating tank; the heating tank A number of ultraviolet sterilizing lamps B are arranged on the upper end surface of the heating tank; the ultraviolet sterilizing lamp B extends into the heating tank; the pumping pipe communicates with the feeding pipe through a pipe. 6. The production device of a kind of vitamin B2 according to claim 5, characterized in that: the tail end of the pumping pipe extends into the heating tank, and is detachably connected with a transparent connecting pipe; the tail of the transparent connecting pipe is close to Heat the bottom of the jar. 7. The production device of a kind of vitamin B2 according to claim 6, characterized in that: the exhaust pipe is also communicated with an exhaust assembly; The exhaust assembly shown includes an exhaust box, and the upper end of the exhaust box is connected with an exhaust inlet pipe and an exhaust outlet pipe; a sealing liquid is also stored in the exhaust box; the tail end of the exhaust inlet pipe extends into the sealing liquid Among them, the tail end of the exhaust outlet pipe is far away from the upper end surface of the sealing liquid, and the exhaust pipe is connected with the exhaust inlet pipe. 8. A vitamin B2 production device according to claim 7, characterized in that: a buffer ball is detachably connected between the exhaust pipe and the exhaust inlet pipe.

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