CN116368156A - 基于从头设计的蛋白开关的模块化和通用化生物传感器平台 - Google Patents

基于从头设计的蛋白开关的模块化和通用化生物传感器平台 Download PDF

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Publication number
CN116368156A
CN116368156A CN202180049976.6A CN202180049976A CN116368156A CN 116368156 A CN116368156 A CN 116368156A CN 202180049976 A CN202180049976 A CN 202180049976A CN 116368156 A CN116368156 A CN 116368156A
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amino acid
seq
reporter
acid sequence
protein
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CN202180049976.6A
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Chinese (zh)
Inventor
阿尔弗雷多·基亚诺·卢比奥
朴周永
页先伟
大卫·贝克
曹龙兴
布赖恩·考文垂
因娜·戈瑞斯尼克
丽莎·科佐多伊
兰斯·约瑟夫·斯图尔特
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University of Washington
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University of Washington
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/001—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/576—Immunoassay; Biospecific binding assay; Materials therefor for hepatitis
    • G01N33/5761—Hepatitis B
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08—RNA viruses
    • G01N2333/165—Coronaviridae, e.g. avian infectious bronchitis virus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/33—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Clostridium (G)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
    • G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
    • G01N2333/4701—Details
    • G01N2333/4712—Muscle proteins, e.g. myosin, actin, protein

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Communicable Diseases (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN202180049976.6A 2020-05-27 2021-05-25 基于从头设计的蛋白开关的模块化和通用化生物传感器平台 Pending CN116368156A (zh)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US202063030836P 2020-05-27 2020-05-27
US63/030,836 2020-05-27
US202063051549P 2020-07-14 2020-07-14
US63/051,549 2020-07-14
US202063067643P 2020-08-19 2020-08-19
US63/067,643 2020-08-19
PCT/US2021/034104 WO2021242780A2 (en) 2020-05-27 2021-05-25 Modular and generalizable biosensor platform based on de novo designed protein switches

Publications (1)

Publication Number Publication Date
CN116368156A true CN116368156A (zh) 2023-06-30

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CN202180049976.6A Pending CN116368156A (zh) 2020-05-27 2021-05-25 基于从头设计的蛋白开关的模块化和通用化生物传感器平台

Country Status (10)

Country Link
US (1) US20230279056A1 (https=)
EP (1) EP4157854A2 (https=)
JP (1) JP2023527786A (https=)
KR (1) KR20230017215A (https=)
CN (1) CN116368156A (https=)
AU (1) AU2021282172A1 (https=)
CA (1) CA3178016A1 (https=)
IL (1) IL298192A (https=)
MX (1) MX2022014917A (https=)
WO (1) WO2021242780A2 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN121049494A (zh) * 2025-08-27 2025-12-02 南昌大学 用于检测伏马毒素B1的dlucCage生物传感器及其制备方法和应用

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4182027A1 (en) * 2020-07-14 2023-05-24 University of Washington Sars-cov-2 inhibitors
US20240309059A1 (en) * 2021-07-07 2024-09-19 National University Corporation Tokyo Medical And Dental University Peptide having anti-viral activity, anti-viral agent comprising said peptide, and method for producing said anti-viral agent
TW202419461A (zh) * 2022-11-01 2024-05-16 華盛頓大學 全新設計的螢光素酶
WO2024102823A2 (en) * 2022-11-09 2024-05-16 The Regents Of The University Of California Systems and methods for detecting and/or screening protein aggregation and/or disaggregation
IT202200025416A1 (it) * 2022-12-13 2024-06-13 Univ Pisa Biosensore per la rilevazione di particelle virali
WO2025101453A1 (en) * 2023-11-07 2025-05-15 University Of Washington De novo design of potent minibinder antagonists of il-6r, gp130, and il1-r1

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015067302A1 (en) * 2013-11-05 2015-05-14 Ecole Polytechnique Federale De Lausanne (Epfl) Sensor molecules and uses thereof
WO2019241438A2 (en) * 2018-06-12 2019-12-19 Promega Corporation Multipartite luciferase
CA3106279A1 (en) 2018-07-19 2020-01-23 University Of Washington De novo design of protein switches

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN121049494A (zh) * 2025-08-27 2025-12-02 南昌大学 用于检测伏马毒素B1的dlucCage生物传感器及其制备方法和应用
CN121049494B (zh) * 2025-08-27 2026-02-27 南昌大学 用于检测伏马毒素B1的dlucCage生物传感器及其制备方法和应用

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Publication number Publication date
JP2023527786A (ja) 2023-06-30
US20230279056A1 (en) 2023-09-07
CA3178016A1 (en) 2021-12-02
AU2021282172A1 (en) 2022-12-15
WO2021242780A3 (en) 2022-02-17
IL298192A (en) 2023-01-01
MX2022014917A (es) 2023-02-22
KR20230017215A (ko) 2023-02-03
EP4157854A2 (en) 2023-04-05
WO2021242780A2 (en) 2021-12-02

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