CN116354756A - A kind of organic fertilizer prepared by using compound microbial agent and method thereof - Google Patents

Abstract

The application belongs to the field of fertilizer preparation, and discloses an organic fertilizer prepared by using a compound bacterial agent and a method thereof. In the preparation method, the bacteria liquid prepared by bacillus subtilis and white rot fungus liquid in a weight ratio of (1-2): 1 is used as a compound bacterial agent, and it is added to poultry manure, rice husk, and crushed rice straw , carry out primary fermentation, then add potassium sulfate and magnesium sulfate, carry out secondary fermentation, and dry to obtain organic fertilizer. The fermentation method adopted in the present application effectively improves the enzyme activity of cellulase, protease, and laccase, thereby significantly improving the composting fermentation effect of agricultural waste and shortening the composting fermentation time. In addition, the raw materials used in the organic fertilizer prepared by the present application are simple and easy to obtain, which can provide crops with nutrients such as potassium, magnesium, and phosphorus required for growth, and can give full play to the role of agricultural waste, effectively alleviating the damage to the ecological environment .

Description

A kind of organic fertilizer prepared by using compound microbial agent and method thereof

technical field

The invention belongs to the field of fertilizer preparation, and in particular relates to an organic fertilizer prepared by using a compound bacterial agent and a method thereof.

Background technique

Agricultural waste refers to organic waste discarded during the entire agricultural production process, including plant residues such as straw and animal residues generated in the fields of animal husbandry and fishery. China is a large agricultural country, and it is also the country that produces the largest amount of agricultural waste. The agricultural waste produced every year is calculated in billions of tons, most of which are directly discarded into the environment without proper treatment, which is also harmful to the ecological environment. had a greater impact. In order to realize the sustainable development of agriculture and practice the concept of green development, the waste utilization of agricultural waste has become a key area of research.

Agricultural waste contains a large amount of resources, and composting and fermenting agricultural waste is one of the main ways of secondary utilization of agricultural waste. However, the current method of composting and fermenting agricultural waste mainly adopts natural fermentation, which takes a long time and It will also consume a lot of manpower. Therefore, researchers have discovered that strains can be used as "accelerators" in the fermentation process, but no special research has been done on the selection of fermentation strains. During the fermentation process, there will be certain antagonism between different fermentation strains. To a certain extent, it affects the effect of fermentation, which further affects the utilization efficiency of agricultural waste.

Contents of the invention

The purpose of the present invention is to solve the deficiencies in the prior art, provide a kind of organic fertilizer and method thereof that utilizes composite bacterial agent to prepare, specifically adopt following technical scheme:

An organic fertilizer prepared by using a compound bacterial agent, the raw materials of which include by weight: 30-50 parts of rice straw, 10-20 parts of poultry manure, 5-8 parts of rice husk, and 2-3 parts of compound bacterial agent 1 part to 3 parts of potassium sulfate, 0.1 part to 0.3 parts of magnesium sulfate; the composite bacterial agent is a bacterial liquid composed of Bacillus subtilis and white rot fungus in a weight ratio of (1-2):1.

The present application adopts the method of mixed fermentation of Bacillus subtilis and white rot fungus, and with (1-2):1 as the preferred ratio, effectively improves the cellulase activity and protease activity of Bacillus subtilis, and white rot The laccase activity of the fungus indicates that not only there is no antagonism between the two bacteria, but there is also a certain synergistic promotion effect, and the relay decomposition is carried out step by step and orderly. When the cellulose-degrading enzymes and proteases secreted by Bacillus subtilis and the laccase secreted by white-rot fungi increase in a large amount, the tight combination between lignins is destroyed, thereby accelerating the decomposition stage of agricultural wastes and making agricultural wastes The compost fermentation effect is significantly improved, and the compost fermentation time is shortened. Moreover, the organic fertilizer prepared by the present application uses rice straw, poultry and livestock manure, and rice husk as fermented raw materials. The raw materials used are simple and easy to obtain, and can provide crops with nutrients such as potassium, magnesium, and phosphorus required for growth, and can give full play to agricultural production. The role of waste can effectively alleviate the damage to the ecological environment.

Preferably, the weight ratio of Bacillus subtilis and white-rot fungus in the composite microbial agent is 2:1. When the production of cellulose-degrading enzymes and proteases secreted by Bacillus subtilis is higher than that of laccase secreted by white-rot fungi, the secretion of laccase can be further stimulated, thereby accelerating the decomposition of rice straw, poultry manure and rice husk The effect greatly shortens the time of compost fermentation.

Preferably, the poultry manure used is pig manure with a water content of 81.5%, organic matter of 15%, nitrogen of 0.6%, phosphorus of 0.4%, and potassium of 0.44%. The nutrient content of pig manure is high, balanced, and the fertilizer effect time is long. It also contains more nitrogen, and the proportion of carbon and nitrogen is relatively small. It is easy to be quickly decomposed by microorganisms, thereby releasing nutrients that can be absorbed and utilized by crops.

The application also provides a method for utilizing the organic fertilizer prepared by the compound microbial agent, comprising the following steps:

Bacillus subtilis and white rot fungus are expanded separately first, and the two bacterial solutions obtained from the expansion are mixed evenly to obtain a compound bacterial agent, which is then added to poultry manure, rice husk, and crushed rice straw. Mix well, carry out primary fermentation, then add potassium sulfate and magnesium sulfate, carry out secondary fermentation, and dry to obtain organic fertilizer.

This application adopts a step-by-step fermentation mode, and the fermentation is divided into primary fermentation and secondary fermentation. Potassium sulfate and magnesium sulfate are added during fermentation to supplement nutrients such as potassium and magnesium for microorganisms, which is beneficial to the growth and reproduction of microorganisms. Through the step-by-step fermentation method, a comfortable living environment can be provided for microorganisms, thereby achieving the purpose of accelerating fermentation.

Preferably, the expansion step of Bacillus subtilis comprises: adding 500 μL of Bacillus subtilis liquid to the sterilized LB medium, and culturing for 12 hours at 30° C. and 150 r/min. Add the hyphae mass of white rot fungus to the sterilized PDA medium, and cultivate it for 3 days at 30°C and 150r/min. Sterilizing the medium in advance and operating in a sterile environment fully ensure the singleness of the strains and effectively avoid the contamination of the strains; and a sealing film is also used during the cultivation process to ensure the ventilation of the strains Sex and non-pollution, so that it can fully grow and reproduce in a constant temperature environment.

Preferably, the concentration of the bacillus subtilis and the white rot fungus obtained from the expansion culture is OD ₆₀₀ =0.5. Add the bacillus subtilis and white rot fungus culture obtained from the expansion into the centrifuge tube, then remove the supernatant by centrifugation, wash twice with 0.9% sodium hypochlorite solution, remove the supernatant, and finally adjust the bacterial suspension to OD ₆₀₀ = 0.5. After removing the medium and adjusting the bacterial suspension to the optimal concentration, the self-function of the strain can be fully exerted, and the synergistic promotion effect of the two strains can be enhanced.

Preferably, the primary fermentation time is 8 days, and the secondary fermentation time is 10-12 days.

Preferably, stirring is required every 8 hours during the primary fermentation and secondary fermentation, and stirring is performed 3 times a day. Regular stirring can make the raw materials fully contact each other, so that microorganisms can accelerate the decomposition of raw materials, and stirring can dissipate heat from fermentation, which is convenient for temperature control.

Preferably, the primary fermentation and secondary fermentation are carried out at a temperature of 25°C. Both the primary fermentation and the secondary fermentation are carried out at 25°C, and the primary fermentation temperature is maintained at 30°C-50°C, which is conducive to the survival and activity of Bacillus subtilis and white rot fungi; the secondary fermentation temperature is maintained at 60°C ℃-65℃, which is conducive to the reproduction of microorganisms and kills bacteria in raw materials such as feces.

The beneficial effects of the application are: the application adopts the method of mixed fermentation of Bacillus subtilis and white rot fungus, which effectively improves the enzyme activity of cellulase, protease, and laccase, thereby significantly improving the yield of agricultural waste. Compost fermentation effect, shorten the compost fermentation time. In addition, the raw materials used in the organic fertilizer prepared by the present application are simple and easy to obtain, which can provide crops with nutrients such as potassium, magnesium, and phosphorus required for growth, and can give full play to the role of agricultural waste, effectively alleviating the damage to the ecological environment .

Detailed ways

The conception of the present invention and the technical effects produced will be clearly and completely described below in conjunction with the embodiments, so as to fully understand the purpose, scheme and effect of the present invention. It should be noted that, in the case of no conflict, the embodiments in the present application and the features in the embodiments can be combined with each other.

Example 1

An organic fertilizer prepared by using a compound bacterial agent, its raw materials include: 40 parts of rice straw (crushed to about 8 mm), 20 parts of poultry manure, 6 parts of rice husk, 3 parts of compound bacterial agent, and 2 parts of potassium sulfate in parts by weight , 0.3 parts of magnesium sulfate, the preparation method of this organic fertilizer specifically comprises the following steps:

S1. Strain expansion:

(1) Pipette 500 μL of Bacillus subtilis liquid into each sterilized LB medium on a sterile operating table, seal the parafilm, put it in a biochemical incubator at 30°C and 150 r/min, and incubate for 12 hours;

(2) Pick white rot fungus hyphae blocks into each sterilized PDA medium on the aseptic operation table, seal the sealing film, put it in a biochemical incubator at 30°C and 150r/min, and incubate for 3 days ;

S2, adjusting the concentration of bacteria solution: add the Bacillus subtilis and white rot fungus bacteria solution obtained in S1 into the centrifuge tube, then remove the supernatant by centrifugation, wash twice with 0.9% sodium hypochlorite solution, remove the supernatant, and then use Adjust the bacterial suspension to OD ₆₀₀ =0.5 with an ultraviolet spectrophotometer;

S3, preparation of composite bacterial agent: the Bacillus subtilis obtained in S2 and the white-rot fungus bacterial liquid are prepared in a weight ratio of 1:1 to prepare a composite bacterial agent (that is, 50 mL of Bacillus subtilis and 50 mL of white-rot fungus are contained in every 100 mL of compound bacterial liquid. ); According to the standard analysis method of GB20287-2006 "Agricultural Microbial Inoculant", it is detected that the cellulase activity in the composite bacterial agent is 145.29U/mL, the protease activity is 86.76U/mL, and the laccase activity is 80.60U/mL;

S4. Preparation of organic fertilizer: Mix the raw materials evenly, perform primary fermentation for 8 days and secondary fermentation for 12 days at 25° C., stir once every 8 hours, stir 3 times a day, and dry to obtain organic fertilizer.

Example 2

An organic fertilizer prepared by using a compound bacterial agent, its raw materials include: 40 parts of rice straw (crushed to about 8 mm), 20 parts of poultry manure, 6 parts of rice husk, 3 parts of compound bacterial agent, and 2 parts of potassium sulfate in parts by weight , 0.3 parts of magnesium sulfate, the preparation method of this organic fertilizer specifically comprises the following steps:

S1. Strain expansion:

(1) Pipette 500 μL of Bacillus subtilis liquid into each sterilized LB medium on a sterile operating table, seal the parafilm, put it in a biochemical incubator at 30°C and 150 r/min, and incubate for 12 hours;

(2) Pick white rot fungus hyphae blocks into each sterilized PDA medium on the aseptic operation table, seal the sealing film, put it in a biochemical incubator at 30°C and 150r/min, and incubate for 3 days ;

S2, adjusting the concentration of bacteria solution: add the Bacillus subtilis and white rot fungus bacteria solution obtained in S1 into the centrifuge tube, then remove the supernatant by centrifugation, wash twice with 0.9% sodium hypochlorite solution, remove the supernatant, and then use Adjust the bacterial suspension to OD ₆₀₀ =0.5 with an ultraviolet spectrophotometer;

S3, preparation of composite bacterial agent: the Bacillus subtilis obtained in S2 and the white-rot fungus bacterium liquid are prepared in a weight ratio of 2:1 to prepare a composite bacterial agent (that is, 80 mL of Bacillus subtilis and 40 mL of white-rot fungus are contained in every 120 mL of compound bacterial liquid. ); According to the standard analysis method of GB20287-2006 "Agricultural Microbial Inoculant", it is detected that the cellulase activity in the composite bacterial agent is 164.34U/mL, the protease activity is 107.02U/mL, and the laccase activity is 82.71U/mL;

S4. Preparation of organic fertilizer: mix the raw materials evenly, perform primary fermentation for 8 days and secondary fermentation for 10 days at 25° C., stir once every 8 hours, stir 3 times a day, and dry to obtain an organic fertilizer.

Comparative example 1

An organic fertilizer prepared by using a microbial agent, the raw materials of which include by weight: 40 parts of rice straw (crushed to about 8mm), 20 parts of poultry manure, 6 parts of rice husk, 3 parts of Bacillus subtilis, 2 parts of potassium sulfate , 0.3 parts of magnesium sulfate, the preparation method of this organic fertilizer specifically comprises the following steps (compared to embodiment 1 and only using Bacillus subtilis as fermentation agent):

S1. Strain expansion: pipette 500 μL of Bacillus subtilis liquid into each sterilized LB medium on a sterile operating table, seal the sealing film, and put it in a biochemical incubator at 30°C and 150r/min. Cultivate for 12 hours;

S2, adjusting the concentration of bacterial solution: add the Bacillus subtilis bacterial solution obtained in S1 into a centrifuge tube, then remove its supernatant by centrifugation, and wash twice with 0.9% sodium hypochlorite solution, remove its supernatant, and then use an ultraviolet spectrophotometer Adjust the bacterial suspension to OD ₆₀₀ =0.5;

S3, preparation of microbial inoculum: the Bacillus subtilis bacterial liquid obtained in S2 is used as fermentation inoculum (effective bacteria number ≥ 500 million/mL); analysis and determination are carried out according to GB 20287-2006 "Agricultural Microbial Inoculant" standard analysis method, The activity of cellulase in the fermentation agent was detected to be 56.20U/mL, the activity of protease was 43.28U/mL, and the activity of laccase was not detected;

S4. Preparation of organic fertilizer: Mix the raw materials evenly, under the condition of 25° C., perform primary fermentation for 8 days, secondary fermentation for 15 days, stir once every 8 hours, stir 3 times a day, and dry to obtain organic fertilizer.

Comparative example 2

An organic fertilizer prepared by using a microbial agent, the raw materials of which include by weight: 40 parts of rice straw (crushed to about 8mm), 20 parts of livestock manure, 6 parts of rice husk, 3 parts of white rot fungi, 2 parts of potassium sulfate , 0.3 parts of magnesium sulfate, the preparation method of this organic fertilizer specifically comprises the following steps (compared to embodiment 1 and only using white rot fungus as fermentation agent):

S1. Strain expansion: pick white rot fungus mycelium blocks into each sterilized PDA medium on the aseptic operation table, seal the sealing film, and put it in a biochemical incubator at 30°C and 150r/min , culture 3d;

S2. Adjusting the concentration of bacterial solution: add the white rot fungus bacterial solution obtained in S1 into a centrifuge tube, then remove the supernatant by centrifugation, wash twice with 0.9% sodium hypochlorite solution, remove the supernatant, and then use an ultraviolet spectrophotometer Adjust the bacterial suspension to OD ₆₀₀ =0.5;

S3, preparation of microbial inoculum: use the white rot fungus liquid obtained in S2 as fermentation inoculum (effective bacteria number ≥ 100 million/mL); analyze and measure according to GB 20287-2006 "Agricultural Microbial Inoculant" standard analysis method, It was detected that the cellulase activity in the fermentation agent was 45.14U/mL, the protease activity was 21.86U/mL, and the laccase activity was 54.17U/mL;

S4. Preparation of organic fertilizer: Mix the raw materials evenly, under the condition of 25° C., perform primary fermentation for 8 days, secondary fermentation for 15 days, stir once every 8 hours, stir 3 times a day, and dry to obtain organic fertilizer.

Comparative example 3

An organic fertilizer prepared by using a compound bacterial agent, its raw materials include: 40 parts of rice straw (crushed to about 8 mm), 20 parts of poultry manure, 6 parts of rice husk, 5 parts of compound bacterial agent, 2 parts of potassium sulfate , 0.3 parts of magnesium sulfate, the preparation method of this organic fertilizer specifically comprises the following steps (compared with embodiment 1, adjusting Bacillus subtilis and white rot fungus bacterium liquid is 4: 1 to prepare composite bacterial agent by weight ratio):

S1. Strain expansion:

(1) Pipette 500 μL of Bacillus subtilis liquid into each sterilized LB medium on a sterile operating table, seal the parafilm, put it in a biochemical incubator at 30°C and 150 r/min, and incubate for 12 hours;

(2) Pick white rot fungus hyphae blocks into each sterilized PDA medium on the aseptic operation table, seal the sealing film, put it in a biochemical incubator at 30°C and 150r/min, and incubate for 3 days ;

S2, adjusting the concentration of bacteria solution: add the Bacillus subtilis and white rot fungus bacteria solution obtained in S1 into the centrifuge tube, then remove the supernatant by centrifugation, wash twice with 0.9% sodium hypochlorite solution, remove the supernatant, and then use Adjust the bacterial suspension to OD ₆₀₀ =0.5 with an ultraviolet spectrophotometer;

S3, preparation of composite bacterial agent: the Bacillus subtilis obtained in S2 and the white-rot fungus bacterium liquid are prepared in a weight ratio of 4:1 to prepare a composite bacterial agent (that is, 80 mL of Bacillus subtilis and 20 mL of white-rot fungus are contained in every 100 mL of compound bacterial liquid. ); According to the standard analysis method of GB20287-2006 "Agricultural Microbial Inoculum", it is detected that the cellulase activity in the composite bacterial agent is 112.93U/mL, the protease activity is 60.65U/mL, and the laccase activity is 68.90U/mL;

S4. Preparation of organic fertilizer: mix the raw materials evenly, and perform primary fermentation for 8 days and secondary fermentation for 13 days at 25° C., stirring once every 8 hours, stirring 3 times a day, and drying to obtain an organic fertilizer.

Comparative example 4

An organic fertilizer prepared by using a compound bacterial agent, its raw materials include: 40 parts of rice straw (crushed to about 8 mm), 20 parts of poultry manure, 6 parts of rice husk, 3 parts of compound bacterial agent, and 2 parts of potassium sulfate in parts by weight , 0.3 parts of magnesium sulfate, the preparation method of this organic fertilizer specifically comprises the following steps (compared with embodiment 1, adjusting bacillus subtilis and sulfur bacteria bacterium liquid is 2: 1 to prepare composite bacterial agent by weight ratio):

S1. Strain expansion:

(1) Pipette 500 μL of Bacillus subtilis liquid into each sterilized LB medium on a sterile operating table, seal the parafilm, put it in a biochemical incubator at 30°C and 150 r/min, and incubate for 12 hours;

(2) Add one piece of sulfur bacteria to each sterilized PDA medium on the aseptic operating table, seal the sealing film, put it in a biochemical incubator at 30°C and 150r/min, and incubate for 3 days;

S2, adjusting the concentration of bacterial solution: add the Bacillus subtilis and sulfur bacteria bacterial solutions obtained in S1 into the centrifuge tube, then remove the supernatant by centrifugation, wash twice with 0.9% sodium hypochlorite solution, remove the supernatant, and then use ultraviolet light Adjust the bacterial suspension to OD ₆₀₀ =0.5 with a spectrophotometer;

S3, preparation of composite bacterial agent: the Bacillus subtilis and sulfur bacteria liquid obtained in S2 are prepared in a weight ratio of 2:1 to prepare a composite bacterial agent (that is, 80 mL of Bacillus subtilis and 40 mL of sulfur bacteria are contained in every 120 mL of composite bacterial liquid); According to the standard analysis method of GB 20287-2006 "Agricultural Microbial Agents", it was detected that the cellulase activity in the composite bacterial agent was 44.20U/mL, the protease activity was 26.51U/mL, and the laccase activity was 53.06 U/mL;

S4. Preparation of organic fertilizer: mix raw materials evenly, perform primary fermentation for 8 days, secondary fermentation for 15 days, stir once every 8 hours, stir 3 times a day, and dry to obtain organic fertilizer.

Comparative example 5

An organic fertilizer prepared by using a compound bacterial agent, its raw materials include: 40 parts of rice straw (crushed to about 8 mm), 20 parts of poultry manure, 6 parts of rice husk, 3 parts of compound bacterial agent, and 2 parts of potassium sulfate in parts by weight , 0.3 parts of magnesium sulfate, the preparation method of this organic fertilizer specifically comprises the following steps (compared with embodiment 1 to adjust sulfur bacteria and white rot fungus bacterium liquid to be 2: 1 to prepare composite bacterial agent by weight ratio):

S1. Strain expansion:

(1) Add one sulfur bacteria tablet to each sterilized PDA medium on the aseptic operating table, seal the sealing film, put it in a biochemical incubator at 30°C and 150r/min, and incubate for 3 days;

(2) Pick white rot fungus hyphae blocks into each sterilized PDA medium on the aseptic operation table, seal the sealing film, put it in a biochemical incubator at 30°C and 150r/min, and incubate for 3 days ;

S2. Adjusting the concentration of bacteria solution: add the sulfur bacteria and white rot fungus bacteria solution obtained in S1 into the centrifuge tube, then remove the supernatant by centrifugation, wash twice with 0.9% sodium hypochlorite solution, remove the supernatant, and then use ultraviolet light Adjust the bacterial suspension to OD ₆₀₀ =0.5 with a spectrophotometer;

S3, preparation of composite bacterial agent: the sulfur bacteria obtained in S2 and the white-rot fungus bacterial liquid are prepared in a weight ratio of 2:1 to prepare a composite bacterial agent (that is, 80 mL of sulfur bacteria and 40 mL of white-rot fungus are contained in each 120 mL of compound bacterial liquid); According to the standard analysis method of GB 20287-2006 "Agricultural Microbial Agents", the cellulase activity in the composite bacterial agent was detected to be 50.30U/mL, the protease activity was 22.34U/mL, and the laccase activity was 28.87 U/mL;

S4. Preparation of organic fertilizer: mix raw materials evenly, perform primary fermentation for 8 days, secondary fermentation for 16 days, stir once every 8 hours, stir 3 times a day, and dry to obtain organic fertilizer.

The present application also detects the oxygen consumption rate of the compost by using a portable dissolved oxygen detector. The detection was carried out at a depth of 50cm on the 3rd day of the primary fermentation and the 8th-16th day of the secondary fermentation respectively. The fermentation degree of the compost was detected according to the oxygen consumption rate, and the results are shown in Table 1 below.

Table 1

As shown in Table 1, the oxygen consumption rate of the primary fermentation of Example 2 (the composite bacterial solution prepared by Bacillus subtilis and the white rot fungus bacterial solution in a weight ratio of 2:1 as the composite microbial agent) is the highest, followed by Example 1, The oxygen consumption rate of other comparative examples is significantly lower than that of Examples, which shows that Bacillus subtilis and white rot fungi can have a synergistic promotion relationship, and then by limiting the ratio of addition, the enzyme activity of cellulase and protease can be effectively improved. and laccase enzyme activity, accelerate the decomposition process of rice straw, poultry manure, and rice husk, thereby shortening the fermentation time and increasing the fermentation rate.

Although the description of the invention has been described in considerable detail and with particular reference to a few described embodiments, it is not intended to be limited to any such details or embodiments, or to any particular embodiment, but should be read by reference The appended claims provide the widest possible interpretation of these claims in view of the prior art, effectively encompassing the intended scope of the present invention. Furthermore, the invention has been described above in terms of embodiments foreseeable by the inventors for the purpose of providing a useful description, while insubstantial modifications of the invention which are not presently foreseeable may still represent equivalent modifications of the invention.

Claims (10)

1. A kind of organic fertilizer that utilizes compound bacterial agent to prepare is characterized in that, its raw material comprises according to weight: 30 parts-50 parts of rice straw, 10 parts-20 parts of poultry manure, 5 parts-8 parts of rice husk, composite 2-3 parts of bacteria agent, 1-3 parts of potassium sulfate, 0.1-0.3 parts of magnesium sulfate; the composite bacteria agent is composed of Bacillus subtilis and white rot fungus in a weight ratio of (1-2):1 liquid. 2. The organic fertilizer according to claim 1, characterized in that, the weight ratio of Bacillus subtilis and white rot fungi in the composite microbial agent is 2:1. 3. The organic fertilizer according to claim 1, characterized in that the poultry manure used is 81.5% water content, 15% organic matter, 0.6% nitrogen, 0.4% phosphorus, and 0.44% potassium manure. 4. A method utilizing the organic fertilizer prepared by compound bacterial agent, is characterized in that, comprises the following steps: Bacillus subtilis and white rot fungus are expanded separately first, and the two bacterial solutions obtained from the expansion are mixed evenly to obtain a compound bacterial agent, which is then added to poultry manure, rice husk, and crushed rice straw. Mix well, carry out primary fermentation, then add potassium sulfate and magnesium sulfate, carry out secondary fermentation, and dry to obtain organic fertilizer. 5. method according to claim 4, is characterized in that, the expansion culture of bacillus subtilis comprises the following steps: Take 500 μL of Bacillus subtilis liquid and add it to the sterilized LB medium, and culture it for 12 hours at 30°C and 150 r/min. 6. The method according to claim 4, characterized in that the expansion of white rot fungi comprises the following steps: Add the hyphae mass of white rot fungus to the sterilized PDA medium, and cultivate it for 3 days at 30°C and 150r/min. 7 . The method according to claim 4 , characterized in that the concentration of the Bacillus subtilis and white rot fungi obtained from the expansion culture is OD ₆₀₀ =0.5. 8. The method according to claim 4, characterized in that, the primary fermentation time is 8 days, and the secondary fermentation time is 10 days-12 days. 9. The method according to claim 8, characterized in that, during the primary fermentation and the secondary fermentation, stirring is required every 8 hours, 3 times a day. 10. The method according to claim 9, characterized in that, both the primary fermentation and the secondary fermentation are carried out at 25°C.