CN116268011A

CN116268011A - Disinfectant and preparation method thereof

Info

Publication number: CN116268011A
Application number: CN202310047531.5A
Authority: CN
Inventors: 陈广川; 于红光; 吴新磊; 陆萌
Original assignee: Tongxi Group Co ltd
Current assignee: Tongxi Group Co ltd
Priority date: 2023-01-31
Filing date: 2023-01-31
Publication date: 2023-06-23

Abstract

The invention provides a disinfectant and a preparation method thereof, wherein the disinfectant comprises the following components in parts by weight: 0.1-0.3 part of quaternary ammonium salt, 5-10 parts of plant source auxiliary bactericide and 73-79 parts of ethanol water solution. The quaternary ammonium salt is added into the ethanol aqueous solution to prepare the composite disinfectant, the ethanol has antibacterial, sterilizing and disinfecting effects, and the addition of a proper amount of quaternary ammonium salt can promote the effect of killing fungi, spores and viruses; the plant source auxiliary bactericide component is natural, safe and reliable, does not harm the environment and human body, and has stronger antibacterial property.

Description

Disinfectant and preparation method thereof

Technical Field

The invention belongs to the technical field of disinfectants, and particularly relates to a disinfectant and a preparation method thereof.

Background

Along with the continuous development of society, people pay more and more attention to healthy life, in daily life, especially in some public places with large people's flow, such as public washrooms, hospitals, hotels and the like, because the people's flow is large, a good living environment is provided for bacteria, bacteria are easy to breed, cross infection of people is caused, and the public places need to be disinfected regularly.

Alcohol is commonly used in life as a disinfectant for surfaces, hands and skin of general objects. Daily cleaning and disinfection are used for preventing illness in a large aspect. It is known that if dirty places are not cleaned in time, bacteria are most likely to grow, and bacteria spread beyond a certain amount and then spread to nearby objects and then to people. Under the influence of new coronaviruses, the disinfection protection consciousness of people is rapidly improved, and the disinfection protection consciousness of people is rapidly improved from simple masks and disinfection solutions to protective masks and various disinfection electric appliances, so that the sales volume of a large number of disinfection products is also driven to be increased.

Disinfectant products with a high number of disinfectant components are selected in life because viruses are immunocompetent and it is difficult to kill superviruses if only one disinfectant component is included. The disinfectant of the chlorine preparation has strong sterilization capability, small irritation, slow and durable sterilization effect, but has little effect on viruses and unstable property, needs to be prepared and used at present, and has high cancer-causing metabolite trichloromethane; iodine disinfectant has good stability, high safety and strong sterilization capability, but has poor biodegradability, and can damage the environment after long-term use. The alcohol disinfectant can kill bacterial propagules and destroy most lipophilic viruses, but has weak sterilization effect and is ineffective to fungi, spores and viruses. The currently known quaternary ammonium salt product can kill bacteria, fungi, spores and viruses, belongs to a high-efficiency disinfectant, and has the advantages of no toxicity, no stimulation and good stability.

Currently, quaternary ammonium salts for efficient sterilization are mainly classified into five categories: double-chain quaternary ammonium salts, multifunctional quaternary ammonium salts (i.e., quaternary ammonium salts containing multiple functional groups), polyamine-based quaternary ammonium salts (i.e., quaternary ammonium salts containing multiple amine groups), and asymmetric single-chain quaternary ammonium salts, as well as silicon-based quaternary ammonium salts.

CN108391673a discloses a method for preparing a nano silver/quaternary ammonium salt composite disinfectant, wherein quaternary ammonium salt in the prepared composite disinfectant exists in a cationic form in an aqueous solution, has strong affinity adsorption force on the surfaces of all bacteria and viruses, and can destroy tissue cells of the bacteria and viruses, but the quaternary ammonium salt used in the composite disinfectant is small molecules, is easy to volatilize, and cannot permanently maintain the sterilizing effect.

CN102387703a discloses disinfectant compositions comprising a biguanide compound comprising one or more biguanide polymers, one or more further active agents selected from the group of mono-quaternary ammonium salts, and one or more further active agents selected from the group of one or more isothiazolone compounds, which are currently being limited in use by europe and which are at risk of allergy, for disinfecting fabrics in contact with the skin, are bacteriostatic preservatives, are not suitable for rapid disinfection and have an undesirable killing effect on viruses.

CN106146835a discloses a preparation method and application of a high molecular quaternary ammonium salt antibacterial agent, in which polyethylenimine is quaternized with short-chain halohydrocarbon (e.g. bromohexane and methyl iodide), but short-chain quaternized polyethylenimine has poor inactivation effect on viruses (influenza virus, human coronavirus) containing lipophilic envelopes.

The antibacterial and antiviral action mechanism of the quaternary ammonium salt is mainly that cations adsorb negatively charged bacteria through the actions of electrostatic force, hydrogen bond force, hydrophobic combination between surfactant molecules and protein molecules and the like, and the negatively charged bacteria are gathered on cell walls to generate a room resistance effect, so that the bacteria are prevented from growing and dying; meanwhile, the hydrophobic alkyl can also act with hydrophilic groups of bacteria to change the permeability of the membrane, so that the cell structure is destroyed to cause dissolution and death of cells.

A series of quaternary ammonium salt sterilizing disinfectants disclosed in the prior art have the problems of low sterilizing rate, sterilizing time period and the like, so that the development of the disinfectant with high sterilizing rate and stable antibacterial performance is a research focus in the field.

Disclosure of Invention

Aiming at the defects of the prior art, the invention aims to provide a disinfectant and a preparation method thereof, which have higher sterilization rate and stable and durable antibacterial performance.

In order to achieve the aim of the invention, the invention adopts the following technical scheme:

in a first aspect, the present invention provides a disinfectant comprising the following components in parts by weight:

0.1 to 0.3 part of quaternary ammonium salt

5-10 parts of plant source auxiliary bactericide

73-79 parts of ethanol water solution.

The quaternary ammonium salt is added into the ethanol aqueous solution to prepare the composite disinfectant, the ethanol has antibacterial, sterilizing and disinfecting effects, and the addition of a proper amount of quaternary ammonium salt can promote the effect of killing fungi, spores and viruses; the plant source auxiliary bactericide component is natural, safe and reliable, does not harm the environment and human body, and has stronger antibacterial property.

The quaternary ammonium salt is 0.1-0.3 part, for example, 0.15 part, 0.2 part, 0.25 part or the like.

The plant-source auxiliary bactericide is 5-10 parts, for example, 6 parts, 7 parts or 9 parts, etc.

The aqueous ethanol solution may be 73 to 79 parts, for example, 74 parts, 76 parts, 78 parts, or the like.

Preferably, the quaternary ammonium salt comprises any one or a combination of at least two of dioctadecyl dimethyl ammonium chloride, didodecyl dimethyl ammonium bromide, didecyl dimethyl ammonium chloride, didecyl dimethyl ammonium bromide or cetyl trimethyl ammonium bromide.

Preferably, the mass percentage of the ethanol in the ethanol water solution is 75-95%, for example, 80%, 85% or 90% and the like.

Other specific point values in the above numerical ranges are selectable, and will not be described in detail here.

Preferably, the plant-derived auxiliary germicide comprises any one or a combination of at least two of aloe extract, tea extract, peony root extract, pomegranate bark extract, mugwort extract or honeysuckle extract.

Preferably, the botanical auxiliary germicide is a combination of aloe vera extract and mugwort extract.

Preferably, the plant-derived auxiliary bactericide comprises 2-3 parts by weight of aloe extract, for example, 2.3 parts, 2.6 parts or 2.9 parts by weight of wormwood extract, for example, 2-3 parts, 2.6 parts or 2.9 parts by weight of wormwood extract.

Preferably, the plant-derived auxiliary germicide is a combination of tea extract and peony root extract.

Preferably, the plant-derived auxiliary bactericide comprises 2-3 parts by weight of tea extract, for example, 2.3 parts, 2.6 parts or 2.9 parts by weight of peony root extract.

Preferably, the plant-derived auxiliary germicide is a combination of pericarpium Granati extract and flos Lonicerae extract.

Preferably, the plant-source auxiliary bactericide comprises 2-3 parts by weight of pomegranate rind extract, for example, 2.3 parts, 2.6 parts or 2.9 parts by weight of honeysuckle extract.

Preferably, the aloe vera extract is prepared using a process comprising: extracting aloe powder to obtain aloe extract, and drying the aloe extract to obtain the aloe extract.

Preferably, the tea extract is prepared by a process comprising: extracting tea leaves to obtain tea leaf extract, and drying the tea leaf extract to obtain the tea leaf extract.

Preferably, the peony root extract is prepared by a method comprising: extracting peony root powder to obtain peony root extract, and drying the peony root extract to obtain the peony root extract.

Preferably, the pericarpium Granati extract is prepared by a method comprising: extracting pericarpium Granati to obtain pericarpium Granati extractive solution, and drying the pericarpium Granati extractive solution to obtain pericarpium Granati extract.

Preferably, the mugwort extract is prepared by a method comprising: extracting the mugwort powder to obtain mugwort extract, and drying the mugwort extract to obtain the mugwort extract.

Preferably, the honeysuckle extract is prepared by a method comprising: extracting honeysuckle to obtain honeysuckle extract, and drying the honeysuckle extract to obtain the honeysuckle extract.

Preferably, the extraction each independently comprises a step of alcohol extraction and water extraction.

Preferably, the temperature of the alcohol extraction is 65-70 ℃, for example, 66 ℃, 67 ℃ or 68 ℃ and the like, and the time is 1-2h, for example, 1.2h, 1.5h or 1.8h and the like.

Preferably, the alcohol extraction agent is ethanol water solution.

Preferably, the mass percentage of the ethanol in the ethanol water solution is 60-75%, for example, 64%, 68% or 73% and the like.

Preferably, the ratio of the ethanol extract to the liquid is 1 (12-18), for example, 1:14, 1:15 or 1:17 can be adopted.

Preferably, the temperature of the water extraction is 80-100deg.C, for example 82 ℃, 84 ℃, 86 ℃, 88 ℃,90 ℃, 92 ℃, 94 ℃, 96 ℃ or 98 ℃, etc., and the time is 1.5-2 hours, for example 1.6 hours, 1.7 hours or 1.9 hours, etc.

Preferably, the water extraction liquid ratio is 1 (9-13), for example, 1:10, 1:11 or 1:12 can be adopted.

In a second aspect, the present invention provides a method of preparing a disinfectant as provided in the first aspect, the method comprising: and uniformly mixing the quaternary ammonium salt, the plant source auxiliary bactericide and the ethanol water solution to obtain the disinfectant.

Compared with the prior art, the invention has the following beneficial effects:

Detailed Description

The technical scheme of the invention is further described by the following specific embodiments. It will be apparent to those skilled in the art that the examples are merely to aid in understanding the invention and are not to be construed as a specific limitation thereof.

The terms "comprising," "including," "having," "containing," or any other variation thereof, are intended to cover a non-exclusive inclusion. For example, a composition, step, method, article, or apparatus that comprises a list of elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, step, method, article, or apparatus.

"optional" or "any" means that the subsequently described event or event may or may not occur, and that the description includes both cases where the event occurs and cases where the event does not.

The indefinite articles "a" and "an" preceding an element or component of the invention are not limited to the requirement (i.e. the number of occurrences) of the element or component. Thus, the use of "a" or "an" should be interpreted as including one or at least one, and the singular reference of an element or component includes the plural reference unless the amount clearly dictates otherwise.

The description of the terms "one embodiment," "some embodiments," "exemplarily," "specific examples," or "some examples," etc., herein described means that a specific feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present invention. In this document, the schematic representations of the above terms are not necessarily for the same embodiment or example.

Preparation example 1

The preparation example provides an aloe extract, and the preparation method of the aloe extract comprises the following steps:

adding 16 times of 70% ethanol water solution into aloe powder, extracting at 70deg.C for 1.5 hr, and filtering to obtain ethanol extractive solution; adding 11 times of water into the aloe powder after the ethanol extraction, extracting for 2 hours at 90 ℃, filtering to obtain an aqueous extract, combining the ethanol extract and the aqueous extract, and freeze-drying to obtain the aloe extract.

Preparation example 2

The preparation example provides a tea extract, and the preparation method of the tea extract comprises the following steps:

adding 15 times of 75% ethanol water solution into green tea, extracting at 70deg.C for 1.5 hr, and filtering to obtain ethanol extractive solution; adding 10 times of water into the green tea after the alcohol extraction, extracting for 2 hours at 90 ℃, filtering to obtain an aqueous extract, combining the alcohol extract and the aqueous extract, and freeze-drying to obtain the tea extract.

Preparation example 3

The preparation example provides a peony root extract, and the preparation method of the peony root extract comprises the following steps:

adding 15 times of 75% ethanol water solution into peony root, extracting at 70deg.C for 1.5 hr, and filtering to obtain ethanol extract; adding 10 times of water into the peony root after the alcohol extraction, extracting for 2 hours at 90 ℃, filtering to obtain an aqueous extract, combining the alcohol extract and the aqueous extract, and freeze-drying to obtain the peony root extract.

Preparation example 4

The preparation example provides a mugwort extract, and the preparation method of the mugwort extract comprises the following steps:

adding 15 times of 75% ethanol water solution into folium Artemisiae Argyi, extracting at 70deg.C for 1.5 hr, and filtering to obtain ethanol extractive solution; adding 10 times of water into the mugwort after the alcohol extraction, extracting for 2 hours at 90 ℃, filtering to obtain an aqueous extract, combining the alcohol extract and the aqueous extract, and freeze-drying to obtain the mugwort extract.

Preparation example 5

The preparation example provides a honeysuckle extract, and the preparation method of the honeysuckle extract comprises the following steps:

adding 15 times of 75% ethanol water solution into flos Lonicerae, extracting at 70deg.C for 1.5 hr, and filtering to obtain ethanol extractive solution; adding 10 times of water into the honeysuckle after the ethanol extraction, extracting for 2 hours at 90 ℃, filtering to obtain an aqueous extract, combining the ethanol extract and the aqueous extract, and freeze-drying to obtain the honeysuckle extract.

Preparation example 6

The preparation example provides a pomegranate rind extract, and the preparation method of the pomegranate rind extract comprises the following steps:

adding 15 times of 75% ethanol water solution into pericarpium Granati, extracting at 70deg.C for 1.5 hr, and filtering to obtain ethanol extractive solution; adding 10 times of water into the pericarpium Granati after the ethanol extraction, extracting for 2 hours at 90 ℃, filtering to obtain an aqueous extract, combining the aqueous extract and the aqueous extract, and freeze-drying to obtain the pericarpium Granati extract.

Example 1

The embodiment provides a disinfectant, which comprises the following components in parts by weight:

0.2 part of dioctadecyl dimethyl ammonium chloride, 2 parts of aloe extract obtained in preparation example 1, 3 parts of wormwood extract obtained in preparation example 4 and 75 parts of 75% ethanol aqueous solution;

the preparation method comprises the following steps: and uniformly mixing all the components according to the formula amount to obtain the disinfectant.

Example 2

0.3 part of didodecyl dimethyl ammonium bromide, 3 parts of tea extract obtained in preparation example 2, 3 parts of peony root extract obtained in preparation example 3 and 74 parts of 90% ethanol water solution;

Example 3

0.1 part of cetyl trimethyl ammonium bromide, 2 parts of honeysuckle extract obtained in preparation example 5, 3 parts of pomegranate rind extract obtained in preparation example 6 and 73 parts of 95% ethanol aqueous solution;

Example 4

0.2 part of dioctadecyl dimethyl ammonium chloride, 5 parts of aloe extract obtained in preparation example 1 and 75 parts of 75% ethanol water solution;

Comparative example 1

The comparative example provides a disinfectant, which comprises the following components in parts by weight:

the difference from example 1 was only 0.5 part of dioctadecyl dimethyl ammonium chloride, and the rest of the raw materials, amounts and preparation methods were the same as in example 1.

Comparative example 2

the difference from example 1 was only 0.05 part of dioctadecyl dimethyl ammonium chloride, and the rest of the raw materials, amounts and preparation methods were the same as in example 1.

Comparative example 3

the difference from example 1 is only that dioctadecyl dimethyl ammonium chloride is not added, and the rest of raw materials, amounts and preparation methods are the same as in example 1.

Comparative example 4

the difference from example 1 is that aloe extract and mugwort extract are not added, and the other raw materials, the amount and the preparation method are the same as in example 1.

Test example 1

Staphylococcus aureus ATCC6538 and escherichia coli 8099 are taken as test strains, the algebra of the strains is 4 th generation, bacterial suspension is prepared by PBS containing 0.03mol/L, 3.9% of D/E neutralization broth is taken as a neutralizing agent, 3% of bovine serum albumin is taken as an organic interference object, and the identification test is carried out according to the neutralizing agent of 2.1.1.5 of the disinfection technical Specification (2002 edition); 2.1.1.7 quantitative bacterial killing test neutralizing agent identification test and killing performance test were performed.

Preparation of bacterial suspension: selecting bacterial suspension with bacterial content of 1X 108 CFU/mL-5X 108 CFU/mL.

Neutralization agent identification test: the test bacteria are staphylococcus aureus. The test groups are as follows: the method comprises the steps of (1) a disinfectant sample+bacterial suspension, (2) (disinfectant sample+bacterial suspension) +neutralizing agent, (3) neutralizing agent+bacterial suspension, (4) (disinfectant sample+neutralizing agent) +bacterial suspension, (5) a diluent+bacterial suspension, (6) a diluent+neutralizing agent+culture medium, wherein the disinfectant sample is prepared from disinfectant stock solution and water according to a mass ratio of 1:49, and the test is repeated for 3 times for 1 min.

And (3) testing the killing performance: according to the quantitative sterilization test of the suspension, preparing a test solution by using disinfectant stock solution and water according to the mass ratio of 1:4, wherein the action time is 5min and 10min, the test is repeated for 3 times, and the test environment temperature is as follows: 23.5 ℃, relative humidity: 60%.

The results of the killing test of the disinfectants prepared in examples 1 to 4 and comparative examples 1 to 4 on staphylococcus aureus and escherichia coli are shown in table 1.

TABLE 1

According to the table data, when the proportion of the quaternary ammonium salt in the disinfectant is too high, the sterilization effect is not obviously increased, the cost is increased, and when the proportion is too low, the sterilization effect is reduced; when the quaternary ammonium salt is not added, the sterilization effect is greatly reduced; when the plant source auxiliary germicide is not added, the germicide effect is reduced.

The applicant states that the process of the invention is illustrated by the above examples, but the invention is not limited to, i.e. it does not mean that the invention has to be carried out in dependence on the above examples. It should be apparent to those skilled in the art that any modification of the present invention, equivalent substitution of raw materials for the product of the present invention, addition of auxiliary components, selection of specific modes, etc., falls within the scope of the present invention and the scope of disclosure.

Claims

1. The disinfectant is characterized by comprising the following components in parts by weight:

0.1 to 0.3 part of quaternary ammonium salt

5-10 parts of plant source auxiliary bactericide

73-79 parts of ethanol water solution.

2. A disinfectant according to claim 1 wherein the quaternary ammonium salt comprises any one or a combination of at least two of dioctadecyl dimethyl ammonium chloride, didodecyl dimethyl ammonium bromide, didecyl dimethyl ammonium chloride, didecyl dimethyl ammonium bromide or cetyl trimethyl ammonium bromide.

3. A disinfectant according to claim 1 or claim 2, wherein the aqueous ethanol solution has a mass percentage of ethanol of 75 to 95%.

4. A disinfectant according to any one of claims 1 to 3, wherein the botanical auxiliary disinfectant comprises any one or a combination of at least two of aloe vera extract, tea leaf extract, peony root extract, pomegranate bark extract, mugwort extract or honeysuckle extract.

5. The disinfectant according to any one of claims 1 to 4, wherein the botanical auxiliary disinfectant is a combination of aloe vera extract and mugwort extract;

preferably, the plant-source auxiliary bactericide comprises, by weight, 2-3 parts of aloe extract and 2-3 parts of wormwood extract;

preferably, the plant-derived auxiliary germicide is a combination of tea extract and peony root extract;

preferably, the plant source auxiliary bactericide comprises 2-3 parts of tea extract and 2-3 parts of peony root extract in parts by weight;

preferably, the plant-derived auxiliary germicide is a combination of a pomegranate rind extract and a honeysuckle extract;

preferably, the plant-source auxiliary bactericide comprises 2-3 parts by weight of pomegranate rind extract and 2-3 parts by weight of honeysuckle extract.

6. The disinfectant according to claim 5, wherein the aloe extract is prepared by a process comprising: extracting aloe powder to obtain aloe extract, and drying the aloe extract to obtain aloe extract;

7. The disinfectant according to claim 5, wherein the peony root extract is prepared by a process comprising: extracting peony root powder to obtain peony root extract, and drying the peony root extract to obtain the peony root extract;

8. The disinfectant according to claim 5, wherein the mugwort extract is prepared by a method comprising: extracting mugwort powder to obtain mugwort extract, and drying the mugwort extract to obtain mugwort extract;

9. A disinfectant according to any one of claims 6 to 8, wherein the extractions each independently comprise the steps of alcohol extraction and water extraction;

preferably, the temperature of the alcohol extraction is 65-70 ℃ and the time is 1-2h;

preferably, the alcohol extraction agent is ethanol water solution;

preferably, the mass percentage of the ethanol in the ethanol water solution is 60-75%;

preferably, the alcohol extraction liquid ratio is 1 (12-18);

preferably, the temperature of the water extraction is 80-100 ℃ and the time is 1.5-2h;

preferably, the water extraction liquid ratio is 1 (9-13).

10. A method of preparing a disinfectant as claimed in any one of claims 1 to 9, wherein the method comprises: and uniformly mixing the quaternary ammonium salt, the plant source auxiliary bactericide and the ethanol water solution to obtain the disinfectant.