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Nutrient solution of calcium tyrphostin
CN1162489A
China
Description
translated from
The invention discloses a kind of nutritional solution of short calcium absorption, especially a kind of nutritional solution of CPP-Ca, it belongs to the food technology field that contains the peptide class.
The a large amount of calcium-supplementing nutritive product or the medicine of Xiao Shouing is the preparation that adds V-D with the calcium element mostly in the market, and its curative effect is limited, and life-time service V-D, finds that clinically V-D poisoning case is not within minority.V-D mediation calcium absorption is the factor that universally acknowledged short calcium absorption utilizes, mainly concentrate duodenum and the interior active absorption calcium ion of upper part of small intestine intestinal in vivo from the effect of V-D pharmacology: V-D, and under pH value 7.8 weak basic conditions of small intestinal bottom, the V-D calcium absorption improving function does not have or is not obvious.Yet the small intestinal bottom is than being again the main place of calcium absorption in the long intestinal, and it accounts for 70～80% of the interior calcium absorption threshold value of body, and its calcium absorption mechanism relies on the interior calcium ion concentration gradient difference of intestinal and finishes.In this intestinal district, position, be alkalescence, be easy to form insoluble calcium phosphate, so just limited the absorption of calcium.There is data to prove, the calcium that human body absorbs from diet, its absorbance is only at 21-26%.Improve the absorbance of calcium, must block the interior calcium of intestinal and combine the insoluble calcium phosphate that forms with phosphoric acid.Yet the various goods of replenishing the calcium of Xiao Shouing have almost been ignored the main region of the interior calcium absorption of small intestinal lower intestine in the body in the market.V-D is inoperative in this zone, so the present goods of replenishing the calcium, and its amount of replenishing the calcium is blindly.
The objective of the invention is to solve the drawback of the above-mentioned goods of replenishing the calcium, provide a kind of and needn't use V-D, and existing calcium-enriching function, the bifunctional nutritional solution food that short calcium absorption function is arranged again, the nutritional solution of this food hinders insoluble calcium phosphate formation in the small intestinal lower intestine district in vivo, it is poor to keep increasing the interior solubility calcium ion concentration gradient of intestinal, and to promote the absorption of calcium, its short calcium absorption ability will reach more than 40%.The CPP-Ca that provides (hereinafter to be referred as CPP-Ca) nutritional solution and method for making thereof are wanted safety, nontoxic pair of effect, and wherein the preparation technology of CPP-Ca will have higher extract yield.
Task of the present invention has following technical scheme to finish, and has developed a kind of nutrient liquid, and this nutritional solution is the compositions that is mixed and is formed by following combination,
Components by weight (part)
Deionized water 100
Soluble calcium salt (in calcium constituent) 0.005～0.5
CPP-Ca????????????????????0.005～0.025
Sweeting agent 0.5～15
Acidic flavoring agent 0.1～0.15
Stabilizing agent 0.01～0.2
V-C????????????????????????0.1～0.5
Antiseptic is an amount of
Flavouring agent is an amount of
Peptide in the above component (CPP): calcium=1: (1～20), through the batch mixed dissolving, filter, ultraviolet disinfection, high pressure homogenize, the dilution standardize solution, bottled product is made in fill, and this product is transparent milky colloid solution.
This nutrient liquid, described soluble calcium salt, it can be a calcium lactate, calcium gluconate, calcium pantothenate, calcium acetate, calcium chloride, wherein one or more.
This nutrient liquid, its peptide chain of forming by 25 aminoacid of described CPP-Ca, its molecular weight 4400, its aminoacid sequence structure is: Arg-Glu-Leu-Glu-Glu-Leu-Asn-Val-Pro-Gly-Glu-Ile-Val-Glu-Serp-Leu-Serp-Serp-Serp-Glu-Glu-Ser-Ile-THr-Arg, this peptide chain includes four Serp (phosphoserine), contain four phosphoric acid residues on this Serp, its biologically active, this residue can form complexation calcium salt-CPP-Ca with the calcium complexation, this CPP-Ca product appearance powder that is creamy white.
This nutrient liquid, the preparation process of described CPP-Ca is:
(a), casein is dissolved in 50～85 ℃ of distilled water by 5～30% inventorys, transferring pH value 7～9, homogenizing operating pressure 8～15MPa high pressure homogenize becomes the pasty state base material;
(b), press enzyme concentration 0.01～0.5% and drop into trypsin, transfer pH value 3～6, under 25～65 ℃ of temperature are bathed 1～6 hour, caseinhydrolysate;
(c), behind the enzymolysis, heat rapidly 90 ℃ and be incubated 5 minutes, the deactivation pancreatin;
(d), separate enzymolysis solution under 6000～10000p.r.m, centrifugal 5～20 minutes, must get supernatant;
(e), supernatant crosses liquid under-4～4 ℃, leaves standstill 12～14 hours, removes not hydrolysis macromole;
(f), press the supernatant volume and drop into CaCL
2, press CaCL
2Material amount 20～40% (W/V), fully stirring reaction is 2～5 hours;
(g), press the reactant liquor volume: ethanol=1: (0.3～2.3) adds dehydrated alcohol, and cohesion generates milky floccule precipitation-CPP-Ca, reclaims ethanol;
(h), separation of C PP-Ca, high speed centrifugation is in 6000～10000p.r.m10～30 minute;
(1), vacuum drying, obtain pure product CPP-Ca at vacuum drying below 60 ℃, crushing packing is standby.
This nutrient liquid, described sweeting agent, it can be a sucrose, lactose, xylitol, fructose syrup, stevioside, wherein one or more.
This nutrient liquid, described acidic flavoring agent, it can be a citric acid, tartaric acid, malic acid, wherein one or more.
This nutrient liquid, described stabilizing agent, it can be an xanthan gum, guar gum, arabic gum, tragcanth, wherein one or more.
This nutrient liquid, described antiseptic, it can be a sorbic acid, natrium malicum.
This nutrient liquid, described essence, it can be a strawberry essence, flavoring orange essence, flavoring pineapple essence, honey peach essence.
The manufacture method of this nutrient liquid is as follows:
The component of this nutritional solution follows these steps to preparation:
(1), will add the sweeting agent of 1/5 formula ratio by the stabilizing agent of prescription weighing earlier, dry powder blend will stir, and inputizations glue jar adds deionized water again, fully dissolves under 50～60 ℃ of temperature;
(2), will drop in the blend tank by the soluble calcium salt of prescription weighing, add deionized water, heat 50～60 ℃, fully dissolving;
(3), will by the prescription weighing CPP-Ca, acidic flavoring agent, antiseptic drops in another blend tank successively, adds deionized water, under 50～60 ℃ of temperature fully the dissolving;
(4), in the sweeting agent input sugar bowl with remaining 4/5 formula ratio, add deionized water under 50～60 ℃ of temperature, stir fully dissolving, again through joining in the jar in the filter input;
(5), with lysate among above-mentioned (one)-(three) jar, join in the jar in behind the pipeline ultraviolet disinfection, pumping into, add an amount of flavouring agent again, mix and make concentrated wiring liquid;
(6), concentrated wiring liquid is pumped into high pressure homogenizer, homogenous mass under 8-25MPa;
(7), the concentrated wiring liquid of homogenizing is pumped into dilute preparing tank, fully wash above-mentioned each jar and homogenizer with 2/5 prescription water consumption, dilute preparing tank is gone in the wash water river, adds the deionized water standardize solution, and it is standby to wait to bottle;
(8), the dilution standardize solution nutritional solution, behind the pipeline ultraviolet disinfection, be filled into bottle again, again through pasteurization, make bottled CPP-Ca nutritional solution after the assay was approved.
Nutritional solution of the present invention, its advantage is: successfully developed CPP-Ca salt and made nutrient oral liquor as food additive, can successfully keep the absorption of intravital solubility calcium amount, through measuring: the normal human shows the digestion calcium absorptivity and reaches 46%, reaches the effect that promotes calcium absorption.This nutritional solution warp: acute toxicological experiment, micronucleus test, Salmonella reversion test, the sperm deformity experiment, total number of bacteria and coliform experiment, these five experimental results show that: do not have any poison and pay effect, edible safety is reliable.The contained CPP-Ca of this nutritional solution is a kind of material of biologically active, and it confirms through isotopic tracing very easily by intestinal absorption: CPP-Ca can be absorbed in the body and be deposited among the skeleton.Because CPP-Ca is present in the small intestinal lower intestine, can stop the formation of insoluble calcium phosphate in the intestinal effectively, so just increase calcium ion concentration in the intestinal, thereby improve the diffusion absorbability of calcium, exist and there is no the V-D effect this moment in the intestinal, reached the function of not using V-D and replenishing the calcium.
The extraction preparation method of the CPP-Ca that the present invention taked, owing to adopt under weak basic condition homogenizing casein solution under the PH7-9, abundant homogenizing by the high pressure short time, casein molecule is well-dispersed in the solution, reach abundant and trypsin contact environment, thereby make enzyme digestion reaction carry out rapidly fully, to improve CPP peptide chain cut-out rate.Adopt the supernatant after the high speed centrifugation separation staticly settles with filter at low temperature to add calcium chloride, stir fully reaction, make the phosphoric acid residue complexation formation CPP-Ca salt on the Serp.Calcium salt biologically active on this peptide chain.Again through dehydrated alcohol with 1: (1-2.3) volume ratio, CPP-Ca is extracted in cohesion, makes extract yield near theoretical extraction ratio 15%, has reached 14.5% good yield.
Embodiments of the invention are as follows: protection scope of the present invention is not limited only among the embodiment.
Nutritional solution of the present invention, the wherein preparation of bioactive substance CPP-Ca, such as table 1 column data:
The preparation example of table 1:CPP-Ca
Nutritional solution of the present invention is specifically implemented prescription, as the listed formulation examples of table 2:
Formulation examples (the unit: kg) of table 2:CPP-Ca nutritional solution
| The instance number component | ?????1 | ????2 | ?????3 | ????4 | ????5 | ????6 | ????7 | ????8 |
| Calcium lactate | ????55 | ????50 | ?????180 | ??150 | ???200 | ??150 | ??200 | ??120 |
| Calcium gluconate | ????200 | ?????100 | ||||||
| Calcium acetate | ?????50 | |||||||
| ????CaCL2 | ??40 | |||||||
| Sucrose | ????250 | ????150 | ?????100 | ??80 | ???80 | |||
| Xylitol | ?????100 | ??120 | ???120 | ??200 | ??280 | |||
| Fructose syrup | ????150 | ?????50 | ??50 | ???50 | ??200 | ??50 | ||
| Citric acid | ????7.5 | ????7.5 | ?????7.5 | ??7.5 | ???7.5 | ??7.5 | ??7.5 | |
| Xanthan gum | ????5 | ????7 | ?????7 | ??5 | ???7 | ??5 | ||
| Guar gum | ??5 | ??3 | ||||||
| Tragcanth | ??5 | |||||||
| ?????V-C | ????5 | ????10 | ?????5 | ??10 | ???10 | ??10 | ??10 | ??10 |
| ????CPP-Ca | ????0.5 | ????0.5 | ?????0.5 | ??0.5 | ???0.8 | ??0.5 | ??0.8 | ??0.8 |
| Sodium benzoate | ????2.5 | ????2.5 | ?????2.5 | ??2.5 | ???2.5 | ??2.5 | ??2.5 | ??2.5 |
| Sorbic acid | ????1 | ????1 | ?????1 | ??1 | ???1 | ??1 | ??1 | ??1 |
| Essence | ????0.001 | ????0.001 | ?????0.001 | ??0.001 | ???0.001 | ??0.001 | ??0.001 | ??0.001 |
| Deionized water (1) | ????5000 | ????5000 | ?????5000 | ??5000 | ???5000 | ??5000 | ??5000 | ??5000 |
CPP-Ca nutritional solution actual production process of the present invention is: producing the 2500L nutritional solution with every batch is example.
(1), earlier get 2.5kg xanthan gum and 25kg sucrose, dry powder blend is even, in the inputization glue jar, adds deionized water 200l, and it is standby to translucent colloid solution to heat 50 ℃ ± 5 ℃ stirring and dissolving;
(2), get the 38.75kg calcium lactate and drop into blend tank, add deionized water 150l, and fully the dissolving back is standby to heat 50 ℃ ± 5 ℃;
(3), get CPP-Ca 0.25kg, citric acid 3.75kg, V-C2.5kg, sodium benzoate 1.25kg, sorbic acid 0.5kg drops in another blend tank successively, adds dried up 200l, it is standby to stir fully dissolving under 50 ℃ ± 5 ℃ temperature;
(4), in the 100kg sucrose input sugar bowl with remainder, add deionized water 100l, stir fully dissolving down, again this sugar juice is pumped into duplex strainer and filter the back and join in the jar in dropping at 50 ℃ ± 5 ℃;
(5), with solution among above-mentioned (one)-(three) jar respectively behind the pipeline ultraviolet disinfection, join in the jar in pumping into, adding 1l strawberry essence in this jar mixes and makes concentrated wiring liquid again;
(6), concentrated wiring liquid 650l is pumped into high pressure homogenizer, under 25MPa, that the concentrated wiring liquid homogenizing is complete;
(7), fully wash above-mentioned each jar and homogenizer, wash water imports dilute preparing tank, adds deionized water standardize solution 2500l again, and it is standby to wait to bottle with the 1000l deionized water;
(8), with the nutritional solution of 2500l dilution, behind the pipeline ultraviolet disinfection, go into filling machine and be filled into bottle, again through pasteurization, make bottled CPP-Ca nutritional solution after the assay was approved.
The CPP-Ca nutritional solution of making, through the human consumption absorption experiment, its experiment is reported as follows:
CPP-Ca human consumption absorption test final report I
One, experimental subject:
Get the students of institute earlier, at 18～22 years old age, health status is good, and 30 are divided into two groups at random as experimental subject, are respectively experimental group and matched group.
Two, The time limit of experiment:
Be divided into the second phase, two days food laundering period, experiment periods two days is gathered into food, feces in experiment periods, to be detected.
Three, experimental foods:
Work up recipe in the whole experiment periods by full price food, the content of fixedly having dinner and amount, all food is most with food for experimental subject.In the experiment periods, every meal is reserved one fen and performs an analysis detection.Wherein, the CPP-Ca10mg of experiment periods lunch, supper experimental group clothes calcium gluconate 400mg and 2 preparations of table 1 example, matched group clothes calcium gluconate 400mg refuses to obey CPP-Ca.
Four, feces collection:
Before and after for oral administration at collection feces respectively with the activated carbon label, receive and adopt experiment periods feces, claim gross weight, mix thoroughly, take out partly and detect, calculate and discharge the Ca total amount.
Five, check and analysis and date processing:
Detection method detects by permanganimetric method, measures simultaneously with quality-control sample GBW-08551 Hepar Sus domestica powder during detection, records data.Learn by statistics and handle, calculate the performance digestibility of human body Ca.Six. experimental result:
Seven, conclusion:
| Dietary Ca amount/day (mg) | Mend Ca amount/day (mg) | Discharge Ca/ day (mg) X ± S | The apparent digestibility X of Ca ± S (%) | ????P | |
| Experimental group | ???????480 | ?????400 | ????523.13±57.77 | ????????40.54±6.56 | ＜0.01 |
| Matched group | ???????480 | ?????400 | ????623.64±112.28 | ????????29.13±12.75 |
This experimental result, the digestibility of experimental group (taking the 10mgCPP-Ca group) Ca is 40.54 ± 6.56%, the digestibility of matched group (not obeying the CPP-Ca group) Ca is 29.13 ± 12.75%.Two groups performance digestibility difference has extremely significantly meaning.
CPP-Ca digestibility test report II
One, experimental subject:
Choose certain students of institute, age 22-24 year, healthy man's 12 experimental subjecies by name are divided into two groups at random, are respectively experimental group and matched group.
Two, The time limit of experiment:
Be divided into the second phase, two days food laundering period, experiment periods two days has been collected food, feces in experiment periods.
Three, experimental foods:
Work up recipe in the whole experiment periods by full price food, the content of fixedly having dinner, all food is most with food for experimental subject.In the experiment periods, every meal is reserved one fen and performs an analysis detection.Breakfast experimental group was taken the CPP-Ca of table 1 example 5 preparations and was contained the Ca beverage every day, and the matched group clothes contain the Ca beverage.
Four, feces collection:
For oral administration with the activated carbon label, collect experiment periods feces, claim gross weight, mix thoroughly, take out part and detect, calculate and discharge the Ca total amount.
Five, component analysis and date processing:
Each sample is made 2 parallel samples, detects by permanganimetric method, learns by statistics and handles, and calculates the performance digestibility of Ca.Six, experimental result:
| Take the photograph Ca amount (mg) | Beverage is mended Ca (mg) | Discharge Ca (mg) X ± S | The apparent digestibility X of Ca ± S (%) | ????P | |
| Experimental group | ????398 | ????567 | ??548.8±21.15 | ??????43.126±2.190 | ＜0.05 |
| Matched group | ????398 | ????480 | ??656.2±103.22 | ??????25.372±11.933 |
Seven, conclusion:
By this experimental result, can illustrate that the digestibility 43.126 ± 2.190 of taking CPP-Ca group Ca is higher than refuses to obey CPP-Ca group 25.372 ± 11.933%, difference has significance.
Claims (10)
Hide Dependent
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Claims (10)
Hide Dependent
translated from
1, a kind of nutrient liquid is characterized in that: this nutritional solution is the compositions that is mixed and is formed by following component,
Components by weight (part)
Deionized water 100
Soluble calcium salt (in calcium constituent) 0.005～0.5
CPP-Ca?????????????????????0.005～0.025
Sweeting agent 0.5～15
Acidic flavoring agent 0.1～0.15
Stabilizing agent 0.01～0.2
V-C?????????????????????????0.1～0.5
Antiseptic is an amount of
Flavouring agent is an amount of
Peptide in the above component (CPP): calcium=1: (1～20), through the batch mixed dissolving, filter, ultraviolet disinfection, high pressure homogenize, the dilution standardize solution, bottled product is made in fill, and this product is transparent milky colloid solution.
2, according to the described nutrient liquid of claim 1, it is characterized in that: described soluble calcium salt, it can be a calcium lactate, calcium gluconate, calcium pantothenate, calcium acetate, calcium chloride, wherein one or more.
3, according to the described nutrient liquid of claim 1, it is characterized in that: its peptide chain of forming by 25 aminoacid of described CPP-Ca, its molecular weight 4400, its aminoacid sequence structure is: Arg-Glu-Leu-Glu-Glu-Leu-Asn-Val-Pro-Gly-Glu-Ile-Val-Glu-Serp-Leu-Serp-Serp-Serp-Glu-Glu-Ser-Ile-THr-Arg, this peptide chain includes four Serp (phosphoserine), contain four phosphoric acid residues on this Serp, its biologically active, this residue can form complexation calcium salt-CPP-Ca with the calcium complexation, this CPP-Ca product appearance powder that is creamy white.
4, according to the described nutrient liquid of claim 3, it is characterized in that: the preparation process of described CPP-Ca is:
(a), casein is dissolved in 50～85 ℃ of distilled water by 5～30% inventorys, transferring pH value 7～9, homogenizing operating pressure 8～15MPa, the condition high pressure homogenize becomes the pasty state base material;
(b), press enzyme concentration 0.01～0.5% and drop into trypsin, transfer pH value 3～6, under 25～65 ℃ of temperature are bathed 1～6 hour, caseinhydrolysate;
(c), behind the enzymolysis, heat rapidly 90 ℃ and be incubated 5 minutes, the deactivation pancreatin;
(d), separate enzymolysis solution under 6000～10000p.r.m, centrifugal 5～20 minutes, must get supernatant;
(e), supernatant spends the night under-4～4 ℃, leaves standstill 12～14 hours, removes not hydrolysis macromole;
(f), press the supernatant volume and drop into CacL
2, throw CacL
2Material amount 20～40% (W/V), fully stirring reaction is 2～5 hours;
(g), press the reactant liquor volume: ethanol=1: (0.3～2.3) adds dehydrated alcohol, and cohesion generates milky floccule precipitation-CPP-Ca, reclaims ethanol;
(h), separation of C PP-Ca, high speed centrifugation is in 6000～10000p.r.m10～30 minute;
(1), vacuum drying, obtain pure product CPP-Ca at vacuum drying below 60 ℃, crushing packing is standby.
5, according to the described nutrient liquid of claim 1, it is characterized in that: described sweeting agent, it can be a sucrose, lactose, xylitol, fructose syrup, stevioside, wherein one or more.
6, according to the described nutrient liquid of claim 1, it is characterized in that: described acidic flavoring agent, it can be a citric acid, tartaric acid, malic acid, wherein one or more.
7, according to the described nutrient liquid of claim 1, it is characterized in that: described stabilizing agent, it can be an xanthan gum, guar gum, arabic gum, tragcanth, wherein one or more.
8, according to the described nutrient liquid of claim 1, it is characterized in that: described antiseptic, it can be a sorbic acid, natrium malicum.
9, according to the described nutrient liquid of claim 1, it is characterized in that: described essence, it can be a strawberry essence, flavoring orange essence, flavoring pineapple essence, honey peach essence.
10, the manufacture method of nutrient liquid according to claim 1 is characterized in that:
The component of this nutritional solution follows these steps to preparation:
(1), will add the sweeting agent of 1/5 formula ratio by the stabilizing agent of prescription weighing earlier, dry powder blend will stir, and inputizations glue jar adds deionized water again, fully dissolves under 50～60 ℃ of temperature;
(2), will drop in the blend tank by the soluble calcium salt of prescription weighing, add deionized water, heat 50～60 ℃, fully dissolving;
(3), will by the prescription weighing CPP-Ca, acidic flavoring agent, antiseptic drops in another blend tank successively, adds deionized water, under 50～60 ℃ of temperature fully the dissolving;
(4), in the sweeting agent input sugar bowl with remaining 4/5 formula ratio, add deionized water under 50～60 ℃ of temperature, stir fully dissolving, again through joining in the jar in the filter input;
(5), with lysate among above-mentioned (one)-(three) jar, join in the jar in behind the pipeline ultraviolet disinfection, pumping into, add an amount of flavouring agent again, mix and make concentrated wiring liquid;
(6), concentrated wiring liquid is pumped into high pressure homogenizer, homogenous mass under 8-25MPa;
(7), the concentrated wiring liquid of homogenizing is pumped into dilute preparing tank, fully wash above-mentioned each jar and homogenizer with 2/5 prescription water consumption, wash water pumps into dilute preparing tank, adds the deionized water standardize solution, and it is standby to wait to bottle;
(8), the dilution standardize solution nutritional solution, behind the pipeline ultraviolet disinfection, be filled into bottle again, again through pasteurization, make bottled CPP-Ca nutritional solution after the assay was approved.