CN116067850A - Blood sedimentation detection method and blood analyzer - Google Patents
Blood sedimentation detection method and blood analyzer Download PDFInfo
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- 239000008280 blood Substances 0.000 title claims abstract description 411
- 210000004369 blood Anatomy 0.000 title claims abstract description 410
- 238000001514 detection method Methods 0.000 title claims abstract description 280
- 238000004062 sedimentation Methods 0.000 title claims abstract description 195
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Abstract
The present disclosure provides a blood sedimentation detection method and a blood analyzer to which the blood sedimentation detection method is applied. The blood sedimentation detection method comprises the following steps: filling a proximal tubing section of a sampling position of the sampling device with a first fluid, then sucking a second fluid from the sampling position, and allowing the first fluid and the second fluid to flow to a detection position under the drive of the liquid path support assembly; determining the sampling volume between the detection position and the sample suction position according to the change condition of the signal detected by the blood sedimentation sensor; the drag volume of the blood segment to be measured from the sample sucking position to the detection position before being detected by the blood sedimentation sensor is determined based on a preset blood segment volume and sampling volume between the distal end of the blood segment to be measured and the detection position when detecting the blood sedimentation. The blood sedimentation detection method can overcome the problem of machine-to-machine difference caused by the manufacturing tolerance and the assembly tolerance of the blood analyzer, thereby achieving the purpose of reducing the blood consumption of blood sedimentation detection.
Description
Technical Field
The disclosure relates to the technical field of medical equipment, in particular to a blood sedimentation detection method and a blood analyzer.
Background
Currently, blood analyzers are commonly used for conventional blood measurement, which can measure basic parameters of blood such as erythrocyte count, white blood cell count and classification (three-class or five-class), platelet count, hemoglobin (HGB) content, erythrocyte Sedimentation Rate (ESR), and some clinically significant parameters calculated from these parameters. However, there are manufacturing and assembly tolerances in the manufacturing process of the blood analyzer, such as the total line volume between the sampling needle and the test line connected thereto, and the blood sedimentation test site on the test line is about + -10% from the suction site of the sampling needle. That is, when the volume between the blood sedimentation detection position and the sampling position of the sampling needle is designed to be 200. Mu.L, the actual position deviation thereof can be up to + -20. Mu.L.
In the conventional blood analyzer, the liquid path motion including the sample sucking amount and the dragging distance of the blood sedimentation measurement blood segment is usually fixed, so that the volume of the blood sedimentation measurement blood segment needs to be increased to ensure that the blood sedimentation measurement blood segment can cover the deviation of the blood sedimentation detection position, thereby greatly increasing the blood consumption for blood sedimentation detection.
Disclosure of Invention
Aiming at the technical problems in the prior art, the disclosure provides a blood sedimentation detection method and a blood analyzer, and the blood sedimentation detection method can overcome the problem of inter-machine difference caused by manufacturing tolerance and assembly tolerance of the blood analyzer, thereby achieving the purpose of reducing blood consumption for blood sedimentation detection.
In a first aspect, an embodiment of the present disclosure provides a blood sedimentation detection method, which is applied to a blood analyzer, where the blood analyzer includes a control device, a sampling device, a blood sedimentation detection assembly, and a liquid path support assembly, the control device is electrically connected with the blood sedimentation detection assembly and the liquid path support assembly, respectively, and the blood sedimentation detection assembly includes a detection pipeline and a blood sedimentation sensor arranged for a detection position on the detection pipeline; the liquid path support component is used for providing liquid path support for the sampling device and the blood sedimentation detection component under the control of the control device; the sampling device is used for collecting a blood sample; the blood sedimentation detection method comprises the following steps: filling a proximal tubing section of a sampling device with a first fluid, then drawing a second fluid from the sampling location, and causing the first and second fluids to flow to the detection location under the drive of the fluid circuit support assembly; determining the sampling volume between the detection position and the sample suction position according to the change condition of the signal detected by the blood sedimentation sensor; determining a dragging volume of the blood segment to be measured from the sample sucking position to the detection position before being detected by the blood sedimentation sensor based on a preset blood segment volume and the sampling volume between a distal end of the blood segment to be measured and the detection position when detecting blood sedimentation.
In a second aspect, an embodiment of the present disclosure provides a blood analyzer, including a control device, a sampling device, a blood sedimentation detection assembly, and a fluid path support assembly, where the control device is electrically connected to the blood sedimentation detection assembly and the fluid path support assembly, respectively, and the blood sedimentation detection assembly includes a detection pipeline and a blood sedimentation sensor disposed for a detection position on the detection pipeline; the liquid path support component is used for providing liquid path support for the sampling device and the blood sedimentation detection component under the control of the control device; the sampling device is used for collecting a blood sample; the control device is configured to: filling a proximal tubing section of a sampling device with a first fluid, then drawing a second fluid from the sampling location, and causing the first and second fluids to flow to the detection location under the drive of the fluid circuit support assembly; determining the sampling volume between the detection position and the sample suction position according to the change condition of the signal detected by the blood sedimentation sensor; determining a dragging volume of the blood segment to be measured from the sample sucking position to the detection position before being detected by the blood sedimentation sensor based on a preset blood segment volume and the sampling volume between a distal end of the blood segment to be measured and the detection position when detecting blood sedimentation.
Compared with the prior art, the beneficial effects of the embodiment of the disclosure are that: according to the method and the device, according to the change condition of the signals corresponding to the first fluid and the second fluid which are sequentially inhaled, the sampling volume of the detection position and the sample suction position of the blood analyzer is determined, and the dragging volume is determined through the sampling volume corresponding to the blood analyzer and the preset blood segment volume, so that the problem that the sampling volumes of all the blood analyzers are different due to the inter-machine difference of the blood analyzers is solved, the dragging volumes corresponding to all the blood analyzers are determined in a targeted mode, and the purpose of reducing the blood sample demand of blood sedimentation detection is achieved.
Drawings
In the drawings, which are not necessarily drawn to scale, like numerals may describe similar components in different views. The same reference numerals with letter suffixes or different letter suffixes may represent different instances of similar components. The accompanying drawings illustrate various embodiments by way of example in general and not by way of limitation, and together with the description and claims serve to explain the disclosed embodiments. Wherever possible, the same reference numbers will be used throughout the drawings to refer to the same or like parts. Such embodiments are illustrative and not intended to be exhaustive or exclusive of the present apparatus or method.
FIG. 1 is a block diagram of a blood analyzer according to an embodiment of the present disclosure;
FIG. 2 is a flow chart of a blood sedimentation detection method according to an embodiment of the present disclosure;
FIG. 3 is a schematic illustration of a proximal tubing segment of a blood analyzer filled with a first fluid in a blood sedimentation detection method of an embodiment of the present disclosure;
FIG. 4 is a schematic diagram of a second fluid drawn into a sampling device flowing through a blood sedimentation sensor in a blood sedimentation detection method according to an embodiment of the present disclosure;
FIG. 5 is a schematic diagram of a sampling device sucking a blood segment to be measured in a blood sedimentation detection method according to an embodiment of the present disclosure;
FIG. 6 is a schematic diagram of dragging a blood segment to be tested to a testing position according to an embodiment of the present disclosure;
FIG. 7 is another flow chart of a blood sedimentation detection method according to an embodiment of the present disclosure;
fig. 8 is a schematic diagram of signal variation in the case where the first fluid is a diluent and the second fluid is air in the blood sedimentation detection method according to the embodiment of the present disclosure.
The reference numerals in the drawings denote components:
100-hematology analyzer; 1-a control device; 2-sampling means; 3-a blood sedimentation detection assembly; 31-detecting a pipeline; a 32-blood sedimentation sensor; 4-liquid path support assembly.
Detailed Description
Various aspects and features of the present invention are described herein with reference to the accompanying drawings.
It should be understood that various modifications may be made to the embodiments of the invention herein. Therefore, the above description should not be taken as limiting, but merely as exemplification of the embodiments. Other modifications within the scope and spirit of the invention will occur to persons of ordinary skill in the art.
The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and, together with a general description of the invention given above, and the detailed description of the embodiments given below, serve to explain the principles of the invention.
These and other characteristics of the invention will become apparent from the following description of a preferred form of embodiment, given as a non-limiting example, with reference to the accompanying drawings.
It is also to be understood that, although the invention has been described with reference to some specific examples, those skilled in the art can certainly realize many other equivalent forms of the invention.
The above and other aspects, features and advantages of the present invention will become more apparent in light of the following detailed description when taken in conjunction with the accompanying drawings.
Specific embodiments of the present invention will be described hereinafter with reference to the accompanying drawings; however, it is to be understood that the inventive embodiments are merely examples of the invention, which may be embodied in various forms. Well-known and/or repeated functions and constructions are not described in detail to avoid obscuring the invention in unnecessary or unnecessary detail. Therefore, specific structural and functional details disclosed herein are not intended to be limiting, but merely as a basis for the claims and as a representative basis for teaching one skilled in the art to variously employ the present invention in virtually any appropriately detailed structure.
The specification may use the word "in one embodiment," "in another embodiment," "in yet another embodiment," or "in other embodiments," which may each refer to one or more of the same or different embodiments in accordance with the invention.
Embodiments of the present disclosure provide a blood sedimentation detection method that is applied to a blood analyzer 100. As shown in fig. 1, the blood analyzer 100 includes a control device 1, a sampling device 2, a blood sedimentation detection unit 3, and a fluid path support unit 5. The control device 1 is respectively and electrically connected with the blood sedimentation detection assembly 3 and the liquid path support assembly 5 so as to control the blood sedimentation detection assembly 3 and the liquid path support assembly 5 to cooperate to execute corresponding instructions. The blood sedimentation detection assembly 3 comprises a detection pipeline 31 and a blood sedimentation sensor 32 which is arranged for a detection position on the detection pipeline 31, wherein the blood sedimentation sensor 32 is electrically connected with the control device 1 so that the control device 1 can receive signals detected by the blood sedimentation sensor 32; the fluid path support assembly 5 is configured to provide fluid path support for the sampling device 2 and the blood sedimentation detection assembly 3 under the control of the control device 1.
In some embodiments, the control device 1 may be implemented in various ways, such as, but not limited to, the control device 1 including at least a processing component, RAM, ROM, a communication interface, a memory, and an I/O interface. The processing components, RAM, ROM, communications interfaces, memory, and I/O interfaces communicate over a bus. The processing component may be a CPU, GPU or other chip with computing capabilities. The memory stores various computer programs such as an operating system and application programs for execution by the processor element and data required for execution of the computer programs. In addition, during the blood sample analysis, data stored locally may be stored in memory if desired. The I/O interface is constituted by a serial interface such as USB, IEEE1394, or RS-232C, a parallel interface such as SCSI, IDE, or IEEE1284, and an analog signal interface composed of a D/a converter, an a/D converter, and the like. An input device consisting of a keyboard, a mouse, a touch screen or other control buttons is connected to the I/O interface, and a user can directly input data to the control apparatus 1 using the input device. In addition, a display device having a display function, for example, may be connected to the I/O interface: liquid crystal screens, touch screens, LED display screens, and the like. The control device 1 may output the processed data as image display data to a display device for display, for example: analytical data, instrument operating parameters, etc. The communication interface is an interface that may be any communication protocol presently known. The communication interface communicates with the outside through a network. The control device 1 can transmit data with any device connected via the network via a communication interface in a certain communication protocol.
The sampling device 2 is configured to collect a blood segment to be measured. Specifically, the sampling device 2 is used to collect a blood sample from a sample container containing the blood sample and distribute the blood sample to the blood sedimentation detection assembly 3. The sampling device 2 may comprise a sampling needle for aspirating a blood segment to be measured.
Further, the blood sedimentation detection assembly 3 comprises a detection pipeline 31 and a blood sedimentation sensor 32 arranged for a detection position on the detection pipeline 31, wherein the detection pipeline 31 is used for providing a detection place for blood sedimentation of a blood segment to be detected. The blood sedimentation sensor 32 may include an optical detection module to irradiate the blood segment to be detected in the detection line 31, so as to determine the erythrocyte sedimentation rate of the blood segment to be detected according to the absorption or scattering degree of the light of the blood segment to be detected in the detection line 31. The blood sedimentation sensor 32 may further include a heater and a temperature sensor, and temperature control of the detection line 31 is achieved by the heater and the temperature sensor.
In some embodiments, the blood analyzer 100 may further include a protein detection module or other detection module for detecting a specific protein, which is not specifically limited herein, and may be adaptively added according to actual detection requirements.
In some embodiments, the fluid circuit support assembly 5 may include functional support for fluid actuation, reagent priming, fluid circuit cleaning, waste fluid drainage, and the like. For example, the liquid path supporting component 5 can respectively provide cleaning liquid for the sampling device 2 and the blood sedimentation detecting component 3, so as to clean the sampling device 2 and the blood sedimentation detecting pipeline 31 respectively, and avoid polluting the blood sample to be detected and leading to inaccurate detection results. The liquid path support component 5 can also be connected with the sampling needle through a pipeline so as to keep the pipeline full of diluent, so that the processes of sampling, sample dividing and the like are realized more quickly and reliably. The fluid circuit support assembly 5 may be a pump, syringe or other source of power, such as a positive or negative air pressure source, or the like.
As shown in fig. 2, the blood sedimentation detection method includes steps S101 to S103.
Step S101: the proximal tubing section of the sampling device 2 is filled with a first fluid and then a second fluid is sucked from the sampling site and the first and second fluids are caused to flow to the detection site by the drive of the fluid circuit support assembly 5.
Specifically, the sampling needle of the sampling device 2 may be directly connected to the detection pipeline 31, or may be connected to the detection pipeline 31 through a section of sample sucking pipeline, which is not limited in this application. The sampling needles of the sampling device 2 shown only by way of example in fig. 3 to 6 are each connected directly to the detection line 31.
Specifically, as shown in fig. 3, fig. 3 is a schematic illustration of the first fluid filling the proximal tubing segment. The proximal tubing segment of the sampling site of the sampling device 2, which may be understood as the site of the needle opening of the sampling needle of the sampling device 2, may be understood as a segment of tubing adjacent to the needle opening of the sampling needle, which may include tubing passing through the blood sedimentation sensor 32, is filled with the first fluid via the fluid circuit support assembly 5.
Specifically, as shown in fig. 4, fig. 4 is a schematic view of the flow of the second fluid drawn into the sampling device 2 past the blood sedimentation sensor 32. After filling the proximal tubing segment with the first fluid, the sampling port of the sampling device 2 is aspirated with the power of the fluid circuit support assembly 5 such that the first and second fluids sequentially flow through the blood sedimentation sensor 32, and the blood sedimentation sensor 32 detects the substance flowing therethrough in real time.
Step S102: a sample volume between the detection location and the sample suction location is determined based on the change in the signal detected by the blood sedimentation sensor 32.
Specifically, the first detection signal corresponding to the first fluid and the second detection signal corresponding to the second fluid detected by the blood sedimentation sensor 32 are different, so that the blood sedimentation sensor 32 can determine the change condition of the signals in the process of real-time detection, that is, the first detection signal is detected in the previous period of time of the blood sedimentation sensor 32, when the time t is reached, the detected first detection signal is suddenly changed into the second detection signal corresponding to the second fluid, and after the control device 1 receives the change condition of the signals detected by the blood sedimentation sensor 32, the volume between the sample sucking position of the sampling device 2 and the detection device, that is, the sampling volume can be determined by calculation or the like.
Thus, the problem that the sampling volume of each blood analyzer 100 has errors due to the difference between machines in the prior art is avoided, but the errors can only be overcome by increasing the blood sample amount to detect the blood sedimentation is solved. The present disclosure, however, can determine the sample volume of each blood analyzer 100 by the change condition of the signal detected by the blood sedimentation sensor 32, which is beneficial to providing a detection environment with higher accuracy for the subsequent blood sedimentation detection.
Step S103: the drag volume of the blood segment to be measured from the sample sucking position to the detection position before being detected by the blood sedimentation sensor 32 is determined based on a preset blood segment volume and the sampling volume between the distal end of the blood segment to be measured and the detection position at the time of detecting the blood sedimentation.
Specifically, the distal end of the blood segment to be measured is understood as the end of the blood segment to be measured near the sampling needle, i.e. the end of the blood segment to be measured. The front end of the blood segment to be measured passes through the blood sedimentation sensor 32 first under the power support of the liquid path support component 5, and then the tail end of the blood segment to be measured passes through the blood sedimentation sensor 32.
As shown in fig. 5 and 6, fig. 5 is a schematic view of the sampling device 2 sucking in a blood segment to be tested, and fig. 6 is a schematic view of dragging the blood segment to be tested to a testing position. After the sampling volume is determined, a dragging volume can be determined according to the sampling volume and a preset blood segment volume, so that the blood segment to be detected sucked by the sampling device 2 is dragged to a detection position by the dragging volume.
The above-mentioned preset blood segment volume is understood as delta marked in fig. 6, i.e. the volume between the distal end of the blood segment to be measured and the detection position. The predetermined blood volume may be predetermined based on experience and experimental data. In the case where the blood segment to be measured is dragged to a volume between its distal end and the detection position that is a preset blood segment volume, a relatively accurate blood sedimentation detection result can be obtained via the blood sedimentation sensor 32.
Therefore, the drag volume is determined based on the preset blood segment volume and the sampling volume, and after the drag volume drags the blood segment to be detected to the detection position, the consistency of detection points on the detected blood segment to be detected can be realized when different blood analyzers 100 detect the blood sedimentation, the requirement on the blood sample volume can be reduced, and the blood segment can be saved.
According to the method and the device, according to the change condition of signals corresponding to the first fluid and the second fluid which are sequentially inhaled, the sampling volume of the detection position and the sample suction position of the blood analyzer 100 is determined, and the dragging volume is determined through the sampling volume corresponding to the blood analyzer 100 and the preset blood segment volume, so that the problem that the sampling volumes of the blood analyzers 100 are different due to the inter-machine difference of the blood analyzers 100 is solved, the dragging volumes corresponding to the blood analyzers 100 are determined in a targeted manner, and the blood sample demand of blood sedimentation detection is reduced.
In some embodiments, the first fluid is a diluent, a hemolyzing agent, a latex reagent, or a quality control substance, and the second fluid is a gas.
In some embodiments, the first fluid is a diluent and the second fluid is a hemolysis agent, a latex reagent, or a quality control substance.
The first fluid is taken as a diluent, and the second fluid is taken as air as an example.
In some embodiments, the first fluid and the second fluid are driven towards the detection means before the sampling means 2 aspirates the blood segment to be measured.
Further, as shown in fig. 7, step S102: according to the change condition of the signal detected by the blood sedimentation sensor 32, the sampling volume between the detection position and the sample suction position is determined, and the method specifically comprises the steps of S201 to S203.
Step S201: a first detection signal of the first fluid and a second detection signal of the second fluid are acquired via the blood sedimentation sensor 32, the second detection signal having a change from the first detection signal.
Step S202: a duration of the first detection signal before changing to a second detection signal is determined.
Step S203: a sampling volume between the detection location and the sample-taking location is determined based on the duration and the flow rate of the first fluid.
Specifically, as shown in fig. 8, fig. 8 is a schematic diagram of signal change in the case where the first fluid is a diluent and the second fluid is air. The signal detected by the blood sedimentation sensor 32 at the initial time is a first detection signal corresponding to the diluent, air is sucked in via the sampling device 2 at the initial time, that is, under the power support of the fluid circuit support assembly 5, and at the time t, the blood sedimentation sensor 32 detects a second fluid, that is, air, in the detection circuit 31. The signals continuously detected by the blood sedimentation sensor 32 after the time t are the second detection signals corresponding to the air.
The control device 1 receives the first detection signal, the second detection signal, and the time t at which the first detection signal, the second detection signal, and the time t are changed, and can determine the duration of the flow of the end of the first fluid from the sample suction position to the detection position.
Specifically, a flow sensor may be disposed on the detection line 31, and the flow sensor transmits a flow rate corresponding to the first fluid to the control device 1, and the control device 1 can determine a sampling volume between the detection position and the sample suction position based on the determined duration and flow rate.
Specifically, the first fluid and the second fluid flow in the detection pipeline 31 under the power support of the liquid path support assembly 5, and the control device 1 can determine the flow rate of the first fluid by acquiring the relevant parameters of the liquid path support assembly 5 for providing power. The method for acquiring the flow of the first fluid is not limited, and the accurate flow of the first fluid can be determined.
Specifically, the sample volume can be calculated using the following formula: p=q×t. Where P is the sample volume, Q is the flow of the first fluid, and t is the duration.
In this way, the present disclosure can accurately calculate the above-described sampling volume by the duration of the first detection signal before changing to the second detection signal and the flow rate of the first fluid, in preparation for a subsequent determination of the drag volume.
In some embodiments, step S103: determining a drag volume of the blood segment to be measured from the sample sucking position to the detection position before being detected by the blood sedimentation sensor 32 based on a preset blood segment volume and the sampling volume between a distal end of the blood segment to be measured and the detection position at the time of detecting blood sedimentation, specifically includes: the drag volume is determined based on a difference between the sampling volume and the preset blood segment volume.
Specifically, the drag volume can be calculated using the following formula: v=p- Δ. Wherein V is the dragging volume, P is the sampling volume, and delta is the preset blood volume. Therefore, the dragging volume is determined based on the difference between the sampling volume and the preset blood segment volume, and after the blood segment to be detected is dragged to the detection position by the dragging volume, the consistency of detection points on the detected blood segment to be detected by different blood analyzers 100 during blood sedimentation detection can be realized, the requirement on the blood sample volume can be reduced, and the blood segment can be saved.
In some embodiments, the blood sedimentation detection method further comprises: placing the blood analyzer 100 into a fluid path initialization mode via the fluid path support assembly 5; and executing a measuring point program for determining the sampling volume under the condition that the liquid path initialization mode is completed. Wherein the station procedure is configured to: filling a proximal tubing section of a sample sucking position of the sampling device 2 with a first fluid, then sucking a second fluid from the sample sucking position, and causing the first fluid and the second fluid to flow to the detection position under the drive of the liquid path support assembly 4; a sample volume between the detection location and the sample suction location is determined based on the change in the signal detected by the blood sedimentation sensor 32.
Specifically, after the blood analyzer 100 is powered on, the blood analyzer 100 drives the diluent to flow in each pipeline through the liquid path supporting component 5, so as to clean the pipelines and the components, avoid polluting the blood segment to be tested and affecting the detection result, i.e. execute the liquid path initialization mode. After the liquid path initialization mode is completed, the subsequent measuring point program is executed. The above-described site procedure can be understood as a procedure in which the sample volume is determined by detecting changes in the signals corresponding to the first fluid and the second fluid by the blood sedimentation sensor 32.
In some embodiments, the blood sedimentation detection method further comprises: receiving a trigger signal for triggering a virtual key on a display interface of the blood analyzer 100; and executing a measuring point program for determining the sampling volume based on the trigger signal. Wherein the station procedure is configured to: filling a proximal tubing section of a sample sucking position of the sampling device 2 with a first fluid, then sucking a second fluid from the sample sucking position, and causing the first fluid and the second fluid to flow to the detection position under the drive of the liquid path support assembly 4; a sample volume between the detection location and the sample suction location is determined based on the change in the signal detected by the blood sedimentation sensor 32.
Specifically, the blood analyzer 100 may further include a display device electrically connected to the control device 1, where the display device has a display interface, and the display interface can present the working condition of the blood analyzer 100, and can clearly present the analysis result to the user. The display interface may also be provided with a virtual key, and triggering the virtual key may generate a trigger signal, and the control device 1 executes a measurement point program after receiving the trigger signal, thereby determining the sampling volume.
Specifically, the above-mentioned execution of the measurement point program may be performed when the blood analyzer 100 is first started, may be performed after each time the blood analyzer 100 is powered on, or may be performed when the user activates the virtual key, and in short, the measurement point program should be performed before determining the dragging volume, so that after determining the sampling volume by the measurement point program, the dragging volume drags the blood segment to be measured, which is sucked by the sampling device 2, to the detection position, and the blood sedimentation detection is performed on the blood segment to be measured via the blood sedimentation sensor 32.
In some embodiments, the blood sedimentation detection method further comprises: receiving a pressing signal for pressing an entity key of the blood analyzer 100; a station procedure for determining the sample volume is performed based on the compression signal. Wherein the station procedure is configured to: filling a proximal tubing section of a sample sucking position of the sampling device 2 with a first fluid, then sucking a second fluid from the sample sucking position, and causing the first fluid and the second fluid to flow to the detection position under the drive of the liquid path support assembly 4; a sample volume between the detection location and the sample suction location is determined based on the change in the signal detected by the blood sedimentation sensor 32.
Specifically, the blood analyzer 100 may further include a physical key electrically connected to the control device 1, where the physical key may be disposed on the body of the blood sedimentation analyzer, and a pressing signal may be generated by pressing the physical key, and the control device 1 receives the pressing signal and executes a measurement point procedure, so as to determine the sampling volume.
In some embodiments, the blood sedimentation detection method further comprises: the determined sample volume is stored via a memory of the blood analyzer 100 after the station procedure is performed. After the memory stores the sample volume, the control device 1 can determine the dragging volume based on the sample volume and the preset blood segment volume before detection, and of course, the sample volume is based on the measurement result of the measurement point program executed on the last side, that is, the sample volume is updated along with the measurement result of the measurement point program executed, so that the dragging volume can be determined based on the last measured sample volume during blood sedimentation measurement.
The presently disclosed embodiments also provide a blood analyzer 100, as shown in fig. 1, the blood analyzer 100 including a control device 1, a sampling device 2, a blood sedimentation detection assembly 3, and a fluid path support assembly 5. The control device 1 is respectively and electrically connected with the blood sedimentation detection assembly 3 and the liquid path support assembly 5 so as to control the blood sedimentation detection assembly 3 and the liquid path support assembly 5 to cooperate to execute corresponding instructions. The blood sedimentation detection assembly 3 comprises a detection pipeline 31 and a blood sedimentation sensor 32 which is arranged for a detection position on the detection pipeline 31, wherein the blood sedimentation sensor 32 is electrically connected with the control device 1 so that the control device 1 can receive signals detected by the blood sedimentation sensor 32; the fluid path support assembly 5 is configured to provide fluid path support for the sampling device 2 and the blood sedimentation detection assembly 3 under the control of the control device 1. The control device 1 is configured to: filling a proximal tubing section of a sample sucking position of the sampling device 2 with a first fluid, then sucking a second fluid from the sample sucking position, and causing the first fluid and the second fluid to flow to the detection position under the drive of the liquid path support assembly 5; determining a sampling volume between the detection location and the sample suction location based on a change in the signal detected by the blood sedimentation sensor 32; the drag volume of the blood segment to be measured from the sample sucking position to the detection position before being detected by the blood sedimentation sensor 32 is determined based on a preset blood segment volume and the sampling volume between the distal end of the blood segment to be measured and the detection position at the time of detecting the blood sedimentation.
In some embodiments, the control device 1 may be implemented in various ways, such as, but not limited to, the control device 1 including at least a processing component, RAM, ROM, a communication interface, a memory, and an I/O interface. The processing components, RAM, ROM, communications interfaces, memory, and I/O interfaces communicate over a bus. The processing component may be a CPU, GPU or other chip with computing capabilities. The memory stores various computer programs such as an operating system and application programs for execution by the processor element and data required for execution of the computer programs. In addition, during the blood sample analysis, data stored locally may be stored in memory if desired. The I/O interface is constituted by a serial interface such as USB, IEEE1394, or RS-232C, a parallel interface such as SCSI, IDE, or IEEE1284, and an analog signal interface composed of a D/a converter, an a/D converter, and the like. An input device consisting of a keyboard, a mouse, a touch screen or other control buttons is connected to the I/O interface, and a user can directly input data to the control apparatus 1 using the input device. In addition, a display device having a display function, for example, may be connected to the I/O interface: liquid crystal screens, touch screens, LED display screens, and the like. The control device 1 may output the processed data as image display data to a display device for display, for example: analytical data, instrument operating parameters, etc. The communication interface is an interface that may be any communication protocol presently known. The communication interface communicates with the outside through a network. The control device 1 can transmit data with any device connected via the network via a communication interface in a certain communication protocol.
The sampling device 2 is configured to collect a blood segment to be measured. Specifically, the sampling device 2 is used to collect a blood sample from a sample container containing the blood sample and distribute the blood sample to the blood sedimentation detection assembly 3. The sampling device 2 may comprise a sampling needle for aspirating a blood segment to be measured.
Further, the blood sedimentation detection assembly 3 comprises a detection pipeline 31 and a blood sedimentation sensor 32 arranged for a detection position on the detection pipeline 31, wherein the detection pipeline 31 is used for providing a detection place for blood sedimentation of a blood segment to be detected. The blood sedimentation sensor 32 may include an optical detection module to irradiate the blood segment to be detected in the detection line 31, so as to determine the erythrocyte sedimentation rate of the blood segment to be detected according to the absorption or scattering degree of the light of the blood segment to be detected in the detection line 31. The blood sedimentation sensor 32 may further include a heater and a temperature sensor, and temperature control of the detection line 31 is achieved by the heater and the temperature sensor.
In some embodiments, the blood analyzer 100 may further include a protein detection module or other detection module for detecting a specific protein, which is not specifically limited herein, and may be adaptively added according to actual detection requirements.
In some embodiments, the fluid circuit support assembly 5 may include functional support for fluid actuation, reagent priming, fluid circuit cleaning, waste fluid drainage, and the like. For example, the liquid path supporting component 5 can respectively provide cleaning liquid for the sampling device 2 and the blood sedimentation detecting component 3, so as to clean the sampling device 2 and the blood sedimentation detecting pipeline 31 respectively, and avoid polluting the blood sample to be detected and leading to inaccurate detection results. The liquid path support component 5 can also be connected with the sampling needle through a pipeline so as to keep the pipeline full of diluent, so that the processes of sampling, sample dividing and the like are realized more quickly and reliably. The fluid circuit support assembly 5 may be a pump, syringe or other source of power, such as a positive or negative air pressure source, or the like.
Specifically, the sampling needle of the sampling device 2 may be directly connected to the detection pipeline 31, or may be connected to the detection pipeline 31 through a section of sample sucking pipeline, which is not limited in this application. The sampling needles of the sampling device 2 shown only by way of example in fig. 3 to 6 are each connected directly to the detection line 31.
Specifically, as shown in fig. 3, fig. 3 is a schematic illustration of the first fluid filling the proximal tubing segment. The proximal tubing segment of the sampling site of the sampling device 2, which may be understood as the site of the needle opening of the sampling needle of the sampling device 2, may be understood as a segment of tubing adjacent to the needle opening of the sampling needle, which may include tubing passing through the blood sedimentation sensor 32, is filled with the first fluid via the fluid circuit support assembly 5.
Specifically, as shown in fig. 4, fig. 4 is a schematic view of the flow of the second fluid drawn into the sampling device 2 past the blood sedimentation sensor 32. After filling the proximal tubing segment with the first fluid, the sampling port of the sampling device 2 is aspirated with the power of the fluid circuit support assembly 5 such that the first and second fluids sequentially flow through the blood sedimentation sensor 32, and the blood sedimentation sensor 32 detects the substance flowing therethrough in real time.
Specifically, the first detection signal corresponding to the first fluid and the second detection signal corresponding to the second fluid detected by the blood sedimentation sensor 32 are different, so that the blood sedimentation sensor 32 can determine the change condition of the signals in the process of real-time detection, that is, the first detection signal is detected in the previous period of time of the blood sedimentation sensor 32, when the time t is reached, the detected first detection signal is suddenly changed into the second detection signal corresponding to the second fluid, and after the control device 1 receives the change condition of the signals detected by the blood sedimentation sensor 32, the volume between the sample sucking position of the sampling device 2 and the detection device, that is, the sampling volume can be determined by calculation or the like.
Thus, the problem that the sampling volume of each blood analyzer 100 has errors due to the difference between machines in the prior art is avoided, but the errors can only be overcome by increasing the blood sample amount to detect the blood sedimentation is solved. The present disclosure, however, can determine the sample volume of each blood analyzer 100 by the change condition of the signal detected by the blood sedimentation sensor 32, which is beneficial to providing a detection environment with higher accuracy for the subsequent blood sedimentation detection.
Specifically, the distal end of the blood segment to be measured is understood as the end of the blood segment to be measured near the sampling needle, i.e. the end of the blood segment to be measured. The front end of the blood segment to be measured passes through the blood sedimentation sensor 32 first under the power support of the liquid path support component 5, and then the tail end of the blood segment to be measured passes through the blood sedimentation sensor 32.
As shown in fig. 5 and 6, fig. 5 is a schematic view of the sampling device 2 sucking in a blood segment to be tested, and fig. 6 is a schematic view of dragging the blood segment to be tested to a testing position. After the sampling volume is determined, a dragging volume can be determined according to the sampling volume and a preset blood segment volume, so that the blood segment to be detected sucked by the sampling device 2 is dragged to a detection position by the dragging volume.
The above-mentioned preset blood segment volume is understood as delta marked in fig. 6, i.e. the volume between the distal end of the blood segment to be measured and the detection position. The predetermined blood volume may be predetermined based on experience and experimental data. In the case where the blood segment to be measured is dragged to a volume between its distal end and the detection position that is a preset blood segment volume, a relatively accurate blood sedimentation detection result can be obtained via the blood sedimentation sensor 32.
Therefore, the drag volume is determined based on the preset blood segment volume and the sampling volume, and after the drag volume drags the blood segment to be detected to the detection position, the consistency of detection points on the detected blood segment to be detected can be realized when different blood analyzers 100 detect the blood sedimentation, the requirement on the blood sample volume can be reduced, and the blood segment can be saved.
According to the method and the device, according to the change condition of signals corresponding to the first fluid and the second fluid which are sequentially inhaled, the sampling volume of the detection position and the sample suction position of the blood analyzer 100 is determined, and the dragging volume is determined through the sampling volume corresponding to the blood analyzer 100 and the preset blood segment volume, so that the problem that the sampling volumes of the blood analyzers 100 are different due to the inter-machine difference of the blood analyzers 100 is solved, the dragging volumes corresponding to the blood analyzers 100 are determined in a targeted manner, and the blood sample demand of blood sedimentation detection is reduced.
In some embodiments, the first fluid is a diluent, a hemolyzing agent, a latex reagent, or a quality control substance, and the second fluid is a gas.
In some embodiments, the first fluid is a diluent and the second fluid is a hemolysis agent, a latex reagent, or a quality control substance.
In some embodiments, the first fluid and the second fluid are driven towards the detection means before the sampling means 2 aspirates the blood segment to be measured. The control device 1 is further configured to: acquiring a first detection signal of the first fluid and a second detection signal of the second fluid via the blood sedimentation sensor 32, the second detection signal having a change from the first detection signal; determining a duration of the first detection signal before changing to a second detection signal; a sampling volume between the detection location and the sample-taking location is determined based on the duration and the flow rate of the first fluid.
Specifically, as shown in fig. 8, fig. 8 is a schematic diagram of signal change in the case where the first fluid is a diluent and the second fluid is air. The signal detected by the blood sedimentation sensor 32 at the initial time is a first detection signal corresponding to the diluent, air is sucked in via the sampling device 2 at the initial time, that is, under the power support of the fluid circuit support assembly 5, and at the time t, the blood sedimentation sensor 32 detects a second fluid, that is, air, in the detection circuit 31. The signals continuously detected by the blood sedimentation sensor 32 after the time t are the second detection signals corresponding to the air.
The control device 1 receives the first detection signal, the second detection signal, and the time t at which the first detection signal, the second detection signal, and the time t are changed, and can determine the duration of the flow of the end of the first fluid from the sample suction position to the detection position.
Specifically, a flow sensor may be disposed on the detection line 31, and the flow sensor transmits a flow rate corresponding to the first fluid to the control device 1, and the control device 1 can determine a sampling volume between the detection position and the sample suction position based on the determined duration and flow rate.
Specifically, the first fluid and the second fluid flow in the detection pipeline 31 under the power support of the liquid path support assembly 5, and the control device 1 can determine the flow rate of the first fluid by acquiring the relevant parameters of the liquid path support assembly 5 for providing power. The method for acquiring the flow of the first fluid is not limited, and the accurate flow of the first fluid can be determined.
Specifically, the sample volume can be calculated using the following formula: p=q×t. Where P is the sample volume, Q is the flow of the first fluid, and t is the duration.
In this way, the present disclosure can accurately calculate the above-described sampling volume by the duration of the first detection signal before changing to the second detection signal and the flow rate of the first fluid, in preparation for a subsequent determination of the drag volume.
In some embodiments, the control device 1 is further configured to: the drag volume is determined based on a difference between the sampling volume and the preset blood segment volume.
Specifically, the drag volume can be calculated using the following formula: v=p- Δ. Wherein V is the dragging volume, P is the sampling volume, and delta is the preset blood volume. Therefore, the dragging volume is determined based on the difference between the sampling volume and the preset blood segment volume, and after the blood segment to be detected is dragged to the detection position by the dragging volume, the consistency of detection points on the detected blood segment to be detected by different blood analyzers 100 during blood sedimentation detection can be realized, the requirement on the blood sample volume can be reduced, and the blood segment can be saved.
In some embodiments, the control device 1 is further configured to: placing the blood analyzer 100 into a fluid path initialization mode via the fluid path support assembly 5; and executing a measuring point program for determining the sampling volume under the condition that the liquid path initialization mode is completed. Wherein the station procedure is configured to: filling a proximal tubing section of a sample sucking position of the sampling device 2 with a first fluid, then sucking a second fluid from the sample sucking position, and causing the first fluid and the second fluid to flow to the detection position under the drive of the liquid path support assembly 4; a sample volume between the detection location and the sample suction location is determined based on the change in the signal detected by the blood sedimentation sensor 32.
Specifically, after the blood analyzer 100 is powered on, the blood analyzer 100 drives the diluent to flow in each pipeline through the liquid path supporting component 5, so as to clean the pipelines and the components, avoid polluting the blood segment to be tested and affecting the detection result, i.e. execute the liquid path initialization mode. After the liquid path initialization mode is completed, the subsequent measuring point program is executed. The above-described site procedure can be understood as a procedure in which the sample volume is determined by detecting changes in the signals corresponding to the first fluid and the second fluid by the blood sedimentation sensor 32.
In some embodiments, the blood analyzer 100 further comprises a display device having a display interface in electrical communication with the control device 1. The control device 1 is further configured to: receiving a trigger signal for triggering a virtual key on a display interface of the blood analyzer 100; and executing a measuring point program for determining the sampling volume based on the trigger signal. Wherein the station procedure is configured to: filling a proximal tubing section of a sample sucking position of the sampling device 2 with a first fluid, then sucking a second fluid from the sample sucking position, and causing the first fluid and the second fluid to flow to the detection position under the drive of the liquid path support assembly 4; a sample volume between the detection location and the sample suction location is determined based on the change in the signal detected by the blood sedimentation sensor 32.
Specifically, the blood analyzer 100 may further include a display device electrically connected to the control device 1, where the display device has a display interface, and the display interface can present the working condition of the blood analyzer 100, and can clearly present the analysis result to the user. The display interface may also be provided with a virtual key, and triggering the virtual key may generate a trigger signal, and the control device 1 executes a measurement point program after receiving the trigger signal, thereby determining the sampling volume.
Specifically, the above-mentioned execution of the measurement point program may be performed when the blood analyzer 100 is first started, may be performed after each time the blood analyzer 100 is powered on, or may be performed when the user activates the virtual key, and in short, the measurement point program should be performed before determining the dragging volume, so that after determining the sampling volume by the measurement point program, the dragging volume drags the blood segment to be measured, which is sucked by the sampling device 2, to the detection position, and the blood sedimentation detection is performed on the blood segment to be measured via the blood sedimentation sensor 32.
In some embodiments, the control device 1 is further configured to: receiving a pressing signal for pressing an entity key of the blood analyzer 100; a station procedure for determining the sample volume is performed based on the compression signal. Wherein the station procedure is configured to: filling a proximal tubing section of a sample sucking position of the sampling device 2 with a first fluid, then sucking a second fluid from the sample sucking position, and causing the first fluid and the second fluid to flow to the detection position under the drive of the liquid path support assembly 4; a sample volume between the detection location and the sample suction location is determined based on the change in the signal detected by the blood sedimentation sensor 32.
Specifically, the blood analyzer 100 may further include a physical key electrically connected to the control device 1, where the physical key may be disposed on the body of the blood sedimentation analyzer, and a pressing signal may be generated by pressing the physical key, and the control device 1 receives the pressing signal and executes a measurement point procedure, so as to determine the sampling volume.
In some embodiments, the control device 1 comprises a memory configured to: after executing the station procedure, the determined sample volume is stored. After the memory stores the sample volume, the control device 1 can determine the dragging volume based on the sample volume and the preset blood segment volume before detection, and of course, the sample volume is based on the measurement result of the measurement point program executed on the last side, that is, the sample volume is updated along with the measurement result of the measurement point program executed, so that the dragging volume can be determined based on the last measured sample volume during blood sedimentation measurement.
Furthermore, although exemplary embodiments have been described herein, the scope thereof includes any and all embodiments having equivalent elements, modifications, omissions, combinations (e.g., of the various embodiments across), adaptations or alterations as pertains to the present application. Elements in the claims are to be construed broadly based on the language employed in the claims and are not limited to examples described in the present specification or during the practice of the present application, which examples are to be construed as non-exclusive. It is intended, therefore, that the specification and examples be considered as exemplary only, with a true scope and spirit being indicated by the following claims and their full scope of equivalents.
The above description is intended to be illustrative and not restrictive. For example, the above-described examples (or one or more aspects thereof) may be used in combination with each other. For example, other embodiments may be used by those of ordinary skill in the art upon reading the above description. In addition, in the above detailed description, various features may be grouped together to streamline the application. This is not to be interpreted as an intention that the disclosed features not being claimed are essential to any claim. Rather, the subject matter of the present application is capable of less than all of the features of a particular disclosed embodiment. Thus, the following claims are hereby incorporated into the detailed description as examples or embodiments, with each claim standing on its own as a separate embodiment, and it is contemplated that these embodiments may be combined with one another in various combinations or permutations. The scope of the application should be determined with reference to the appended claims, along with the full scope of equivalents to which such claims are entitled.
The above embodiments are only exemplary embodiments of the present application and are not intended to limit the present application, the scope of which is defined by the claims. Various modifications and equivalent arrangements may be made to the present application by those skilled in the art, which modifications and equivalents are also considered to be within the scope of the present application.
Claims (18)
1. The blood sedimentation detection method is characterized by being applied to a blood analyzer, wherein the blood analyzer comprises a control device, a sampling device, a blood sedimentation detection assembly and a liquid path support assembly, the control device is respectively and electrically connected with the blood sedimentation detection assembly and the liquid path support assembly, and the blood sedimentation detection assembly comprises a detection pipeline and a blood sedimentation sensor which is arranged aiming at a detection position on the detection pipeline; the liquid path support component is used for providing liquid path support for the sampling device and the blood sedimentation detection component under the control of the control device; the sampling device is used for collecting a blood sample; the blood sedimentation detection method comprises the following steps:
filling a proximal tubing section of a sampling device with a first fluid, then drawing a second fluid from the sampling location, and causing the first and second fluids to flow to the detection location under the drive of the fluid circuit support assembly;
determining the sampling volume between the detection position and the sample suction position according to the change condition of the signal detected by the blood sedimentation sensor;
determining a dragging volume of the blood segment to be measured from the sample sucking position to the detection position before being detected by the blood sedimentation sensor based on a preset blood segment volume and the sampling volume between a distal end of the blood segment to be measured and the detection position when detecting blood sedimentation.
2. The method of claim 1, wherein the first fluid and the second fluid are driven to flow to the testing device before the sampling device draws into the blood segment to be tested;
according to the change condition of the signal detected by the blood sedimentation sensor, determining the sampling volume between the detection position and the sample suction position specifically comprises the following steps:
acquiring a first detection signal of the first fluid and a second detection signal of the second fluid via the blood sedimentation sensor, the second detection signal having a change from the first detection signal;
determining a duration of the first detection signal before changing to a second detection signal;
a sampling volume between the detection location and the sample-taking location is determined based on the duration and the flow rate of the first fluid.
3. The method of claim 2, wherein the first fluid is a diluent, a hemolyzing agent, a latex reagent, or a quality control substance, and the second fluid is a gas.
4. The method of claim 2, wherein the first fluid is a diluent and the second fluid is a hemolyzing agent, a latex reagent, or a quality control substance.
5. The method according to claim 1, wherein determining the drag volume of the blood segment to be measured from the sample sucking position to the detection position before the detection by the blood sedimentation sensor based on the preset blood segment volume and the sampling volume between the distal end of the blood segment to be measured and the detection position at the time of detecting blood sedimentation, specifically comprises:
the drag volume is determined based on a difference between the sampling volume and the preset blood segment volume.
6. The blood sedimentation detection method according to claim 2, characterized in that the blood sedimentation detection method further comprises:
placing the blood analyzer into a fluid path initialization mode via the fluid path support assembly;
executing a measuring point program for determining the sampling volume under the condition that the liquid path initialization mode is completed; wherein,
the station procedure is configured to: filling a proximal tubing section of a sampling device with a first fluid, then drawing a second fluid from the sampling location, and causing the first and second fluids to flow to the detection location under the drive of the fluid circuit support assembly; and determining the sampling volume between the detection position and the sample suction position according to the change condition of the signal detected by the blood sedimentation sensor.
7. The blood sedimentation detection method according to claim 1, characterized in that the blood sedimentation detection method further comprises:
receiving a trigger signal for triggering a virtual key on a display interface of the blood analyzer;
executing a measurement point program for determining the sampling volume based on the trigger signal; wherein,
the station procedure is configured to: filling a proximal tubing section of a sampling device with a first fluid, then drawing a second fluid from the sampling location, and causing the first and second fluids to flow to the detection location under the drive of the fluid circuit support assembly; and determining the sampling volume between the detection position and the sample suction position according to the change condition of the signal detected by the blood sedimentation sensor.
8. The blood sedimentation detection method according to claim 1, characterized in that the blood sedimentation detection method further comprises:
receiving a pressing signal for pressing an entity key of the blood analyzer;
performing a station procedure for determining the sample volume based on the compression signal; wherein,
the station procedure is configured to: filling a proximal tubing section of a sampling device with a first fluid, then drawing a second fluid from the sampling location, and causing the first and second fluids to flow to the detection location under the drive of the fluid circuit support assembly; and determining the sampling volume between the detection position and the sample suction position according to the change condition of the signal detected by the blood sedimentation sensor.
9. The blood sedimentation detection method of any one of claims 6-8, further comprising:
the determined sample volume is stored via a memory of the blood analyzer after execution of the site measurement procedure.
10. The blood analyzer comprises a control device, a sampling device, a blood sedimentation detection assembly and a liquid path support assembly, wherein the control device is respectively and electrically connected with the blood sedimentation detection assembly and the liquid path support assembly, and the blood sedimentation detection assembly comprises a detection pipeline and a blood sedimentation sensor which is arranged aiming at a detection position on the detection pipeline; the liquid path support component is used for providing liquid path support for the sampling device and the blood sedimentation detection component under the control of the control device; the sampling device is used for collecting a blood sample; the control device is configured to:
filling a proximal tubing section of a sampling device with a first fluid, then drawing a second fluid from the sampling location, and causing the first and second fluids to flow to the detection location under the drive of the fluid circuit support assembly;
determining the sampling volume between the detection position and the sample suction position according to the change condition of the signal detected by the blood sedimentation sensor;
Determining a dragging volume of the blood segment to be measured from the sample sucking position to the detection position before being detected by the blood sedimentation sensor based on a preset blood segment volume and the sampling volume between a distal end of the blood segment to be measured and the detection position when detecting blood sedimentation.
11. The blood analyzer of claim 10, wherein the first fluid and the second fluid are driven toward the detection device before the sampling device draws into the blood segment to be measured;
the control device is further configured to:
acquiring a first detection signal of the first fluid and a second detection signal of the second fluid via the blood sedimentation sensor, the second detection signal having a change from the first detection signal;
determining a duration of the first detection signal before changing to a second detection signal;
a sampling volume between the detection location and the sample-taking location is determined based on the duration and the flow rate of the first fluid.
12. The hematology analyzer of claim 11, wherein the first fluid is a diluent, a hemolyzing agent, a latex reagent or a quality control substance and the second fluid is a gas.
13. The hematology analyzer of claim 11, wherein the first fluid is a diluent and the second fluid is a hemolysis agent, a latex reagent or a quality control substance.
14. The blood analyzer of claim 10, wherein the control device is further configured to:
the drag volume is determined based on a difference between the sampling volume and the preset blood segment volume.
15. The blood analyzer of claim 11, wherein the control device is further configured to:
placing the blood analyzer into a fluid path initialization mode via the fluid path support assembly;
executing a measuring point program for determining the sampling volume under the condition that the liquid path initialization mode is completed; wherein,
the station procedure is configured to: filling a proximal tubing section of a sampling device with a first fluid, then drawing a second fluid from the sampling location, and causing the first and second fluids to flow to the detection location under the drive of the fluid circuit support assembly; and determining the sampling volume between the detection position and the sample suction position according to the change condition of the signal detected by the blood sedimentation sensor.
16. The blood analyzer of claim 10, further comprising a display device electrically connected to the control device, the display device having a display interface;
the control device is further configured to:
receiving a trigger signal for triggering a virtual key on a display interface of the blood analyzer;
executing a measurement point program for determining the sampling volume based on the trigger signal; wherein,
the station procedure is configured to: filling a proximal tubing section of a sampling device with a first fluid, then drawing a second fluid from the sampling location, and causing the first and second fluids to flow to the detection location under the drive of the fluid circuit support assembly; and determining the sampling volume between the detection position and the sample suction position according to the change condition of the signal detected by the blood sedimentation sensor.
17. The blood analyzer of claim 10, wherein the control device is further configured to:
receiving a pressing signal for pressing an entity key of the blood analyzer;
performing a station procedure for determining the sample volume based on the compression signal; wherein,
The station procedure is configured to: filling a proximal tubing section of a sampling device with a first fluid, then drawing a second fluid from the sampling location, and causing the first and second fluids to flow to the detection location under the drive of the fluid circuit support assembly; and determining the sampling volume between the detection position and the sample suction position according to the change condition of the signal detected by the blood sedimentation sensor.
18. The blood analyzer of any one of claims 15-17, wherein the control device includes a memory configured to:
after executing the station procedure, the determined sample volume is stored.
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