CN115966258A

CN115966258A - Method for screening IRES based on levenstan distance and polynucleotides screened thereby

Info

Publication number: CN115966258A
Application number: CN202111435528.8A
Authority: CN
Inventors: 侯强波; 朱佳凤; 仇宗浩; 左炽健; 孙振华
Original assignee: Suzhou Kerui Maide Biomedical Technology Co ltd
Current assignee: Suzhou Kerui Maide Biomedical Technology Co ltd
Priority date: 2021-10-12
Filing date: 2021-11-29
Publication date: 2023-04-14

Abstract

The disclosure belongs to the technical field of bioinformatics and bioengineering, and particularly relates to a method for screening IRES based on a Levenstan distance, a polynucleotide obtained by screening based on the method, a circular nucleic acid molecule containing the polynucleotide, a cyclization precursor nucleic acid molecule, a recombinant expression vector, a recombinant host cell and application. According to the method, the average value of the Levenstan distances between all sample sequences and the sequence to be predicted is compared, whether the IRES exists in the sequence to be predicted can be efficiently and accurately judged, and the method has the advantages of high efficiency and accurate screening result; moreover, the IRES screened by the IRES prediction method provided by the disclosure has high activity, and provides abundant translation initiation elements for applications of the circular nucleic acid molecules in preparation of proteins, vaccines, production of therapeutic proteins or gene therapy means.

Description

Method for screening IRES based on Levensan distance and polynucleotide screened thereby

Technical Field

The present disclosure belongs to the technical field of bioinformatics and bioengineering, and specifically, the present disclosure relates to a use of a polynucleotide in initiating translation of a circular nucleic acid molecule, a polynucleotide having a translation activity of initiating the circular nucleic acid molecule, a circular nucleic acid molecule comprising the polynucleotide, a circularization precursor nucleic acid molecule, a recombinant expression vector, a recombinant host cell, and a use thereof.

Background

Messenger Ribonucleic Acid (mRNA) is transcribed from DNA and provides the genetic information needed for the next translation of a protein. After mRNA coding the antigen protein is injected into a human body, the mRNA can synthesize the antigen protein in the human body, thereby inducing strong cellular immunity and humoral immunity reaction, showing the characteristics of an autoimmune adjuvant, and being an excellent vaccine means. In addition, mRNA has other advantages as a vaccine or for the production of therapeutic proteins, for example, mRNA is transiently expressed in cells, has no risk of integration into the genome, and is independent of the cell cycle, thus having higher safety, compared to DNA vectors; compared with viral vectors, mRNA has no immune resistance brought by the vectors, so that protein expression is easier to realize; compared with recombinant protein, virus and the like, the production process of mRNA is a cell-free system and only relates to in vitro enzyme catalysis reaction, so that the production process is simpler, controllable and low in cost. Currently, mRNA exhibits broad application potential as a vaccine, for the production of therapeutic proteins, as a means of gene therapy, and the like.

The mRNA currently used clinically or preclinically is mainly linear mRNA, and the structure of linear mRNA includes a 5' cap structure (5 ' Cap), a 3' polyadenylic tail (PolyA tail), a 5' untranslated sequence (5 ' untranslated region,5' UTR), a 3' untranslated sequence (3 ' untranslated region,3' UTR), and an Open Reading Frame (ORF), etc. The 5'cap structure is an essential feature of eukaryotic mRNA and is obtained by adding N7-methylguanosine to the 5' end of mRNA. The research finds that the 5' cap structure promotes mRNA translation by combining with a translation initiation complex eif4E, can effectively prevent mRNA degradation and reduce the immunogenicity of mRNA. The main function of the 3' polyadenylation tail is through binding to PolyA binding proteins (PABPs), which interact with eiF4G and eiF4E, mediating mRNA formation into loops, and promoting translation processes, preventing mRNA degradation. 5 'and 3' untranslated sequences, such as those using beta-globin, are effective in preventing mRNA degradation and promoting translation of mRNA into protein.

Circular RNAs (circular RNAs) are a common type of RNA found in eukaryotes. Naturally occurring circRNAs are produced primarily by an intracellular molecular mechanism called "reverse splicing". It has now been found that eukaryotic circRNAs have a variety of molecular cell regulatory functions. For example, circular RNAs can regulate expression of target genes by binding to microRNAs (mirnas); the circular RNA can regulate gene expression by directly binding to a target protein. The circular RNA that has been identified so far functions mainly as a non-coding RNA. However, circular RNAs encoding proteins, i.e., circular mRNAs, also exist in nature. Cyclic mRNA tends to have a longer half-life due to its cyclic nature, and thus it is presumed that cyclic mRNA may have better stability. Methods for forming circular RNA in vivo include chemical methods, protease-catalyzed methods, and ribozyme-catalyzed methods.

An Internal Ribosome Entry Site (IRES) is a cis-acting RNA sequence that can recruit ribosomal subunits to the translation initiation site of mRNA and mediate the translation process in viruses, certain eukaryotes, etc., without relying on a 5' end cap structure. Circular RNAs are closed circular structures lacking typical translation initiation elements, but still can exert translation functions through IRES-mediated ribosome binding to mRNA. Compared with linear mRNA, the circular mRNA has high stability and has important application prospect in protein expression and clinical treatment. The protein expression level of the circular mRNA molecules is influenced by the translation initiation element, so that more IRES elements capable of initiating the translation of the circular mRNA molecules are found, and the method has important significance for improving the protein expression level of the circular mRNA molecules and expanding the application of the circular mRNA molecules in clinical and industrial production.

At present, because the confirmation, action mechanism research and structure research of IRES in the sequence mainly depend on experimental verification, and the screening of IRES active sequences for a large number of sequences with unknown functions consumes a large amount of time and cost, the number of IRES discovered and verified at present is small, and the application of the circular RNA molecule in aspects of protein expression, clinical treatment and the like is limited.

Disclosure of Invention

Problems to be solved by the invention

In view of the problems in the prior art, for example, the screening of sequences comprising IRES is time-consuming and costly, resulting in a small number of IRES sequences that have been validated at present, limiting the applications of circular mRNA molecules in protein expression, clinical therapy, and the like. Therefore, the method for screening the IRES based on the Levenstan distance can efficiently and quickly screen the sequence to be predicted containing the IRES, has accurate screening result, and is beneficial to discovery of new IRES sequences.

In some embodiments, the present disclosure provides a polynucleotide comprising any one of the nucleotide sequences set forth in (i), wherein the polynucleotide is capable of initiating a translation process of a circular nucleic acid molecule, and wherein the IRES activity is high, wherein the polynucleotide is capable of increasing the protein expression level of the circular nucleic acid molecule, and wherein the polynucleotide provides abundant translation initiation elements for further use of the circular nucleic acid molecule.

Means for solving the problems

In a first aspect, the present disclosure provides a method of screening IRES based on levenstein distance, comprising the steps of:

(1) Selecting n sequences containing IRES as sample sequences; wherein n is more than or equal to 1 and n is a natural number;

(2) Respectively carrying out One-Hot coding on the sample sequence and the sequence to be predicted, wherein the classification variables are A, T, C and G;

(3) Traversing the sample sequences, and calculating the Levensan distance between each sample sequence and the sequence to be predicted;

(4) Calculating the mean value of the Levensan distances between all sample sequences and the sequence to be predicted;

(5) And judging whether the sequence to be predicted comprises the IRES or not based on the average value.

In some embodiments, the method for screening IRES based on levenstein distance according to the present disclosure, wherein in step (5), if the mean value is not less than the set prediction threshold, the sequence to be predicted is determined to contain IRES, otherwise the sequence to be predicted is determined to not contain IRES.

In some embodiments, the method of screening for IRES based on levenstein distance according to the present disclosure, wherein the prediction threshold is not less than 0.5.

In some embodiments, the method of screening for IRES based on levenstein distance according to the present disclosure, wherein the prediction threshold is 0.75.

In some embodiments, a method of screening for IRES based on levenstan distance according to the present disclosure, wherein the method further comprises the steps of:

and traversing the sample sequences and respectively searching the longest common substring of each sample sequence and the sequence to be predicted if the sequence to be predicted is judged to contain the IRES.

In some embodiments, a method of screening for IRES based on levenstein distance according to the present disclosure, wherein said method further comprises the steps of:

predicting the secondary structure of the sequence to be predicted containing the IRES, and combining the longest common substring to judge the position of the IRES in the sequence to be predicted.

In some embodiments, the method for screening IRES based on levenstein distance according to the present disclosure, wherein predicting the secondary structure of a sequence to be predicted that is judged to comprise an IRES comprises: predicting the secondary structure of the sequence to be predicted which is judged to contain the IRES by using at least one of RNA software of RNAfold, mfold, RNAfold, vienna and Vienna.

In some embodiments, the method for screening an IRES based on levenstein distance according to the present disclosure, wherein the secondary structure of the sequence to be predicted that is judged to comprise the IRES is predicted using RNAfold software.

In some embodiments, a method of screening for IRES based on levenstan distance according to the present disclosure, wherein the method further comprises the steps of: and carrying out experimental verification on the sequence to be predicted which is judged to contain the IRES, and judging the IRES activity of the sequence to be predicted.

In some embodiments, the method of screening for IRES based on levenstan distance according to the present disclosure, wherein the step of experimentally validating comprises:

constructing a circular nucleic acid molecule using the sequence to be predicted determined to comprise the IRES, wherein the sequence to be predicted is operably linked to a nucleotide sequence encoding a fluorescent protein;

and acquiring a fluorescence signal released by the circular nucleic acid molecule, and judging the IRES activity of the sequence to be predicted according to the fluorescence signal.

In a second aspect, the present disclosure provides a polynucleotide, wherein the polynucleotide is selected from at least one of the following groups (i) - (iv):

(i) <xnotran> SEQ ID NO:1, 2, 3, 4, 9, 10, 11, 13, 14, 15, 17, 18, 19, 20, 25, 26, 27, 28, 41, 42, 45, 46, 51, 56, 59, 72, 79, 91, 98, 101, 104, 106, 107, 110, 115, 116, 117, 118, 119, 122, 123, 125, 127, 129, 130, 135, 139, 165, 179, 180, 183, 186, 188, 198, 200, 215, 216, 217, 218, 219, 220, 221, 222, 223, 225, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 239, 240, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 272, 273, 274, 275, 276, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 289, 291, 293, 294, 296, 298, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 314, 315, 317, 318, 319, 321, 322, 323, 324, 326, 329, 331, 332, 333, 334, 335, 336, 348, 385, 387, 389, 392, 393, 394, 395, 406, 436, 438, 439, 441, 445, 457, 460, 496, 504, 507, 509, 511, 514, 534 ; </xnotran>

(ii) (ii) a mutant sequence of any one of the nucleotide sequences set forth in (i) having a mutated nucleotide at one or more positions corresponding to any one of the nucleotide sequences set forth in (i) and having the translational activity of the starting circular nucleic acid molecule;

(iii) (iii) a nucleotide sequence which is reverse complementary to the hybridizing sequence of the nucleotide sequence shown in (i) or (ii) under high stringency hybridization conditions or very high stringency hybridization conditions and has the translation activity of the initiating circular nucleic acid molecule;

(iv) (iii) a nucleotide sequence having at least 70%, optionally at least 80%, preferably at least 90%, more preferably at least 95%, most preferably at least 98% sequence identity to the nucleotide sequence set forth in any one of (i) or (ii) and having the translational activity of the initiating circular nucleic acid molecule;

preferably, the polynucleotide comprises a nucleotide sequence set forth in any one of seq id nos: .

In some embodiments, the polynucleotide of the present disclosure, wherein the polynucleotide is a polynucleotide comprising an IRES screened according to the method of any one of claims 1-9.

In some embodiments, the polynucleotides according to the present disclosure are as follows (a) ₁ )-(a ₂ ) Use in at least one of:

(a ₁ ) Initiating translation of the circular nucleic acid molecule, or preparing a product for initiating translation of the circular nucleic acid molecule;

(a ₂ ) Increasing the protein expression level of the circular nucleic acid molecule, or preparing a product for increasing the protein expression level of the circular nucleic acid molecule.

In a third aspect, the present disclosure provides a circular nucleic acid molecule, wherein the circular nucleic acid molecule comprises the polynucleotide of the second aspect;

preferably, the circular nucleic acid molecule further comprises a coding region encoding a polypeptide of interest, said coding region being operably linked to the polynucleotide;

optionally, the circular nucleic acid molecule further comprises one or more of the following elements: a 5 'spacer, a 3' spacer, a second exon, a first exon.

In some embodiments, the circular nucleic acid molecules provided by the present disclosure, the 5' spacer comprises the following (b) ₁ )-(b ₂ ) Any one of the sequences set forth in:

(b ₁ ) The nucleotide sequence shown as any sequence of SEQ ID NO 549-550;

(b ₂ ) And (b) ₁ ) A sequence whose nucleotide sequence as set forth has at least 90%, alternatively at least 95%, preferably at least 97%, more preferably at least 98%, most preferably at least 99% sequence identity.

In some embodiments, the circular nucleic acid molecules provided by the present disclosure, the 3' spacer comprises (c) ₁ )-(c ₂ ) Any one of the sequences set forth in:

(c ₁ ) The nucleotide sequence shown as any sequence of SEQ ID NO. 551-553;

(c ₂ ) And (c) ₁ ) The nucleotide sequence has at least 90%, optionally at least 95%, preferably at least 97%, more preferably at least 98%, most preferably at least 99% sequence identity.

In some embodiments, the circular nucleic acid molecule provided by the present disclosure, the second exon comprises the following (d) ₁ )-(d ₂ ) Any one of the sequences set forth in:

(d ₁ ) A nucleotide sequence shown as SEQ ID NO. 555;

(d ₂ ) And (d) ₁ ) A sequence whose nucleotide sequence as set forth has at least 90%, alternatively at least 95%, preferably at least 97%, more preferably at least 98%, most preferably at least 99% sequence identity.

In some embodiments, the disclosure provides a circular nucleic acid molecule, wherein the first exon comprises the following (e) ₁ )-(e ₂ ) Any one of the sequences set forth in seq id no:

(e ₁ ) A nucleotide sequence shown as SEQ ID NO. 554;

(e ₂ ) And (e) ₁ ) A sequence whose nucleotide sequence as set forth has at least 90%, alternatively at least 95%, preferably at least 97%, more preferably at least 98%, most preferably at least 99% sequence identity.

In a fourth aspect, the present disclosure provides a circularised precursor nucleic acid molecule, wherein said circularised precursor nucleic acid molecule is circularised to form a circular nucleic acid molecule as described in the third aspect;

optionally, the circularized precursor nucleic acid molecule further comprises one or more of the following elements:

a 5 'homology arm, a 3' intron, a second exon, a 5 'spacer, a coding region, a 3' spacer, a first exon, a 5 'intron, and a 3' homology arm.

In some embodiments, the 5' homology arm comprises the following (g) ₁ )-(g ₂ ) Any one of the sequences set forth in:

(g ₁ ) A nucleotide sequence as set forth in any one of SEQ ID NOs 558-559;

(g ₂ ) And (g) ₁ ) Nucleotide sequence shownSequences having at least 90%, alternatively at least 95%, preferably at least 97%, more preferably at least 98%, most preferably at least 99% sequence identity.

In some embodiments, the circularized precursor nucleic acid molecules provided by the present disclosure, the 3' homology arm comprises (h) as follows ₁ )-(h ₂ ) Any one of the sequences set forth in:

(h ₁ ) A nucleotide sequence shown as any one of SEQ ID NO 560-561;

(h ₂ ) And (h) ₁ ) A sequence wherein the nucleotide sequence is at least 90%, alternatively at least 95%, preferably at least 97%, more preferably at least 98%, most preferably at least 99% sequence identity.

In some embodiments, the circularized precursor nucleic acid molecules provided by the present disclosure, the 5' intron comprises (j) as follows ₁ )-(j ₂ ) Any one of the sequences set forth in seq id no:

(j ₁ ) A nucleotide sequence shown as SEQ ID NO. 556;

(j ₂ ) And (j) ₁ ) A sequence whose nucleotide sequence as set forth has at least 90%, alternatively at least 95%, preferably at least 97%, more preferably at least 98%, most preferably at least 99% sequence identity.

In some embodiments, the circularized precursor nucleic acid molecules provided by the present disclosure, the 3' intron comprises (k) as follows ₁ )-(k ₂ ) Any one of the sequences set forth in:

(k ₁ ) A nucleotide sequence as shown in any one of SEQ ID NO: 557;

(k ₂ ) And (k) ₁ ) A sequence wherein the nucleotide sequence is at least 90%, alternatively at least 95%, preferably at least 97%, more preferably at least 98%, most preferably at least 99% sequence identity.

In a fifth aspect, the present disclosure provides a recombinant nucleic acid molecule, wherein the recombinant nucleic acid molecule is selected from the group consisting of (f) ₁ )-(f ₂ ) Any one of the following:

(f ₁ ) Comprising the polynucleotide according to the second aspect,

(f ₂ ) Transcribing to form the circularised precursor nucleic acid molecule of the fourth aspect.

In a sixth aspect, the present disclosure provides a recombinant expression vector, wherein the recombinant expression vector comprises the recombinant nucleic acid molecule of the fifth aspect.

In a seventh aspect, the present disclosure provides a recombinant host cell, wherein the recombinant host cell comprises a polynucleotide according to the second aspect, a circular nucleic acid molecule according to the third aspect, a circularised precursor nucleic acid molecule according to the fourth aspect, a recombinant nucleic acid molecule according to the fifth aspect, or a recombinant expression vector according to the sixth aspect.

In an eighth aspect, the present disclosure provides a method of making a circular nucleic acid molecule with an increased level of expression of a protein, wherein the method comprises the step of operably linking the polynucleotide of the second aspect to a coding region of the circular nucleic acid molecule.

In a ninth aspect, the present disclosure provides a circular nucleic acid molecule according to the third aspect, a circularized precursor nucleic acid molecule according to the fourth aspect, a recombinant nucleic acid molecule according to the fifth aspect, or a recombinant expression vector according to the sixth aspect as follows (g) ₁ )-(g ₃ ) Use in at least one of:

(g ₁ ) Expressing the protein, or preparing a product for expressing the protein;

(g ₂ ) Expressing the polypeptide, or preparing a product for expressing the polypeptide;

(g ₃ ) As or in the preparation of nucleic acid vaccines;

optionally, the protein or polypeptide is selected from one or more of: antigens, antibodies, antigen binding fragments, channel proteins, receptors, cytokines, immune checkpoint inhibitors.

ADVANTAGEOUS EFFECTS OF INVENTION

In some embodiments, the method for screening the IRES based on the levenstan distance provided by the disclosure can efficiently and accurately judge whether the IRES exists in the sequence to be predicted, and if the IRES exists, the position of the IRES can be further predicted and positioned by predicting the secondary structure of the sequence to be predicted and combining the secondary structure with the longest common substring of the sequence and the sample sequence, so that possible IRES core sequences in the sequence can be screened out, technical support is provided for screening the IRES with high activity, discovery of new IRES sequences is facilitated, researchers can selectively perform experimental verification on RNA sequences with high probability of existence of the IRES sequences, experimental verification efficiency is improved, and ineffective time and cost are saved.

In some embodiments, the polynucleotide represented by any one of SEQ ID NO 1 to 548 is screened by the methods provided by the present disclosure. Experiments prove that the polynucleotides shown as any sequence of SEQ ID NO. 1-548 have the activity of starting the translation of circular nucleic acid molecules, which shows that the screening method provided by the disclosure has the advantage of high accuracy.

In some embodiments, the present disclosure finds by comparison that when a polynucleotide comprising a nucleotide sequence as defined in any one of (i) is selected according to the method of the present disclosure, the IRES activity of the polynucleotide exceeds that of the CVB3 IRES element having a high translation initiation activity found so far, the protein expression level of the circular nucleic acid molecule can be significantly increased, and the circular nucleic acid molecule is provided with abundant translation initiation elements for use in preparing proteins, as vaccines, producing therapeutic proteins, or as gene therapy means.

In some embodiments, the present disclosure provides a circular nucleic acid molecule comprising a polynucleotide having a nucleotide sequence shown in (i), which can achieve high-level expression of a target polypeptide or protein, and further expand the application effects in the fields of protein production, prevention or treatment of clinical diseases, and the like.

In some embodiments, the present disclosure operably links a polynucleotide as set forth in any of (i) with a coding region of a circular nucleic acid molecule to provide a good basis for efficient expression of a target protein by the circular nucleic acid molecule.

Drawings

FIG. 1 shows an agarose gel electrophoresis of a portion of Linear mRNA molecules and circular mRNA molecules prepared in example 2, wherein the bands indicated by Linear IRES 1 to 30 in the figure sequentially represent electrophoresis bands of Linear mRNA molecules comprising polynucleotides having sequences indicated by SEQ ID NOS 1 to 30, and the bands indicated by circular IRES 1 to 30 in the figure sequentially represent electrophoresis bands of circular mRNA molecules comprising polynucleotides having sequences indicated by SEQ ID NOS 1 to 30.

FIG. 2 shows agarose gel electrophoresis of a portion of Linear mRNA molecules and circular mRNA molecules prepared in example 2, wherein the bands indicated by Linear IRES 31-62 in the figure sequentially represent electrophoresis bands of Linear mRNA molecules comprising polynucleotides having sequences indicated by SEQ ID NOS: 31-62, and the bands indicated by circular IRES 31-62 in the figure sequentially represent electrophoresis bands of circular mRNA molecules comprising polynucleotides having sequences indicated by SEQ ID NOS: 31-62.

FIG. 3 shows agarose gel electrophoresis of a portion of Linear mRNA molecules and circular mRNA molecules prepared in example 2, wherein the bands indicated by Linear IRES 63-94 in the figure sequentially represent electrophoresis bands of Linear mRNA molecules comprising polynucleotides of sequences indicated by SEQ ID NOS 63-94, and the bands indicated by circular IRES 63-94 in the figure sequentially represent electrophoresis bands of circular mRNA molecules comprising polynucleotides of sequences indicated by SEQ ID NOS 63-94.

FIG. 4 shows the results of detection of fluorescent protein expression by a circular mRNA molecule constructed using the polynucleotide obtained by the method of screening for an IRES according to the present disclosure, wherein the column diagram in the figure is, in order from left to right, control 1, control 2, and a circular mRNA molecule comprising the nucleotide sequence shown in SEQ ID NOS: 1, 2, 3, 4, 9, 10, 11, 13, 14, 15, 17, 18, 19, 20, 25, 26, 27, 28, 41, 42, 45, 46, 51, 56, 59, 72, 79, 91.

FIG. 5 shows the results of detection of fluorescent protein expression by a circular mRNA molecule constructed using the polynucleotide obtained by the method for screening IRES of the present disclosure, wherein the column diagram in the figure is, in order from left to right, control 1, control 2, and a circular mRNA molecule comprising the nucleotide sequence shown in SEQ ID NOS: 98, 101, 104, 106, 107, 110, 115, 116, 117, 118, 119, 122, 123, 125, 127, 129, 130, 135, 139, 165, 179, 180, 183, 186, 188, 198, 200, 215.

FIG. 6 shows the results of detection of fluorescent protein expression in circular mRNA molecules constructed using the polynucleotides obtained by the method of screening for IRES of the present disclosure, wherein the column diagrams in the figure are, in order from left to right, control 1, control 2, and circular mRNA molecules comprising the nucleotide sequences shown in SEQ ID NOS: 216, 217, 218, 219, 220, 221, 222, 223, 225, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 239, 240, 242, 243, 244, 245, 246, 247, 248.

FIG. 7 shows the results of detection of fluorescent protein expression by a circular mRNA molecule constructed using the polynucleotides obtained by the method of screening for IRES according to the present disclosure, wherein the column diagrams in the figure are, in order from left to right, control 1, control 2, and a circular mRNA molecule comprising the nucleotide sequence shown in SEQ ID NOS: 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 272, 273, 274, 275, 276, 278, 279, 280.

FIG. 8 shows the results of detection of fluorescent protein expression from a circular mRNA molecule constructed using the polynucleotides obtained by the method of screening IRES according to the present disclosure, wherein the column diagrams in the figure are, in order from left to right, control 1, control 2, and a circular mRNA molecule comprising the nucleotide sequence shown in

SEQ ID NOS

281, 282, 283, 284, 285, 286, 287, 289, 291, 293, 294, 296, 298, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 314, 315, 317.

FIG. 9 shows the results of detection of fluorescent protein expression by a circular mRNA molecule constructed using the polynucleotide constructed by the method for screening an IRES according to the present disclosure, wherein the circular mRNA molecules having the nucleotide sequences shown in

SEQ ID NOS

318, 319, 321, 322, 323, 324, 326, 329, 331, 332, 333, 334, 335, 336, 348, 385, 387, 389, 392, 393, 394, 395, 406, 436, 438, 439, 441, 445, 457, 460, 496, 504, 507, 509, 511, 514, 534 are sequentially represented in the column diagrams from left to right.

FIG. 10 shows a secondary structure diagram of the Human poliovirus 1string Mahoney _CDC5' UTR sequence predicted in this disclosure and where the IRES is present;

FIG. 11 is a graph showing the results of detection of expression of luciferase from Human polivirus 1strain Mahoney v CDC 5' UTR group, human Echovirus29strain JV-10 group and Human Coxsackievirus B3 group.

Detailed Description

Definition of

The words "a" or "an" when used in conjunction with the term "comprising" in the claims and/or the specification may mean "one", but may also mean "one or more", "at least one", and "one or more than one".

As used in the claims and specification, the words "comprising," "having," "including," or "containing" are intended to be inclusive or open-ended and do not exclude additional, unrecited elements or method steps.

Throughout this specification, the term "about" means: a value includes the standard deviation of error for the device or method used to determine the value.

Although the disclosure supports the definition of the term "or" as merely an alternative and "and/or," the term "or" in the claims means "and/or" unless it is explicitly stated that only alternatives or mutual exclusions between alternatives are mutually exclusive.

The term "One-Hot encoding", also known as One-bit-efficient encoding, mainly uses an N-bit status register to encode N states, each state being represented by its own independent register bit and having only One bit active at any time. One-Hot encoding is the representation of classification variables as binary vectors. This first requires mapping the classification values to integer values. Each integer value is then represented as a binary vector, which is a zero value, except for the index of the integer, which is marked as 1.

The term "traverse sample sequence" sample sequence is an object (or element) that is arranged in a column, with each element either preceding or succeeding other elements. The order between the elements is very important. Traversing the sample sequence is to make one and only one visit to each element in the sample sequence in turn along a certain search route. The operations performed to access an element depend on the specific application problem. Traversal sequences are often used in tree and graph searches of data structures.

The term "levenstein distance" is a distance measure of two sequences of strings. Formally, the levenstein distance of two strings is the minimum number of individual character edits (e.g., deletions, insertions, and substitutions) required to change one string to another. The levenstein distance is also referred to as the edit distance, although it is only one of the edit distances, closely related to paired string alignments. Mathematically, the levenstein distance between two strings a, b, levab (i, j) = max (i, j); if min (i, j) =0; levab (i, j) = min (levab (i-1, j) +1, levab (i, j-1) +1, levab (i-1, j-1) + 1) (ai! = bj), where ai! = bj is an indicator function when ai! = bj is 1, otherwise 0. Note that in min terms: the first part corresponds to a delete operation (from a to b), the second part corresponds to an insert operation, and the third part corresponds to a replace operation.

The term "longest common substring" is the substring for which two or more known strings are sought to be the longest. The longest common sub-string differs from the longest common sub-sequence in that the sub-sequences do not have to be contiguous, but the sub-strings do.

The terms "polypeptide", "peptide" and "protein" are used interchangeably herein and are polymers of amino acids of any length. The polymer may be linear or branched, it may comprise modified amino acids, and it may be interrupted by non-amino acids. The term also includes amino acid polymers that have been modified (e.g., disulfide bond formation, glycosylation, lipidation, acetylation, phosphorylation, or any other manipulation, such as conjugation with a labeling component).

The term "polynucleotide" or "nucleic acid molecule" refers to a polymer composed of nucleotides. Polynucleotides may be in the form of individual fragments, or may be a component of a larger nucleotide sequence structure, derived from nucleotide sequences that have been isolated at least once in quantity or concentration, and which are capable of being recognized, manipulated, and recovered in sequence, and their component nucleotide sequences, by standard molecular biology methods (e.g., using cloning vectors). When a nucleotide sequence is represented by a DNA sequence (i.e., A, T, G, C), this also includes an RNA sequence (i.e., A, U, G, C) in which "U" replaces "T". In other words, a "polynucleotide" refers to a polymer of nucleotides removed from other nucleotides (either individually or as a whole) or may be an integral part or component of a larger nucleotide structure, such as an expression vector or a polycistronic sequence. Polynucleotides include DNA, RNA, and cDNA sequences.

The term "circular nucleic acid molecule" refers to a nucleic acid molecule that is in the shape of a closed loop. In some specific embodiments, the circular nucleic acid molecule is a circular RNA molecule. More specifically, the circular nucleic acid molecule is a circular mRNA molecule.

In some embodiments, the circular RNA molecules in the present disclosure are circular by joining the upstream 5 'end to the downstream 3' end of the linear RNA molecule. The circular RNA molecules in the present disclosure form a circle from a circularized precursor RNA molecule through a cleavage and circularization reaction.

The term "linear RNA" refers to an RNA precursor capable of circularizing to form circular RNA, which is typically transcribed from a linear DNA molecule.

The term "linear RNA" refers to an RNA having a translation function, which includes a 5' cap structure (5 ' Cap), a 3' polyadenylic tail (PolyA tail), a 5' untranslated sequence (5 ' untranslated region,5' UTR), a 3' untranslated sequence (3 ' untranslated region,3' UTR), and an Open reading frame (Open reading frame, ORF).

The term "translation initiation element" refers to any sequence element capable of recruiting ribosomes to initiate the translation process of an RNA molecule. Exemplary translation initiation elements are IRES elements, m ⁶ A modified sequence, or the initiation sequence of rolling circle translation, etc.

The term "IRES" (Internal ribosome entry site, IRES) is also known as an Internal ribosome entry site, "Internal ribosome entry site" (IRES) belongs to a translation control sequence, usually located at the 5' end of a gene of interest, and allows translation of RNA in a cap-independent manner. The transcribed IRES can bind directly to the ribosomal subunit so that the mRNA start codon is properly oriented in the ribosome for translation. The IRES sequence is typically located in the 5' UTR of the mRNA (directly upstream of the start codon). IRES functionally replaces the need for various protein factors that interact with eukaryotic translation machinery.

The term "Coding Region" refers to a gene sequence capable of transcribing messenger RNA and ultimately translating into a polypeptide, protein of interest.

The term "expression" includes any step involved in the production of a polypeptide, including but not limited to: transcription, post-transcriptional modification, translation, post-translational modification, and secretion.

The terms "sequence identity" and "percent identity" refer to the percentage of nucleotides or amino acids that are identical (i.e., identical) between two or more polynucleotides or polypeptides. Sequence identity between two or more polynucleotides or polypeptides can be determined by: the nucleotide or amino acid sequences of the polynucleotides or polypeptides are aligned and the number of positions in the aligned polynucleotides or polypeptides containing the same nucleotide or amino acid residue are scored and compared to the number of positions in the aligned polynucleotides or polypeptides containing different nucleotide or amino acid residues. The polynucleotides may differ at one position, for example, by containing different nucleotides (i.e., substitutions or mutations) or deleted nucleotides (i.e., nucleotide insertions or nucleotide deletions in one or both polynucleotides). Polypeptides may differ at one position, for example, by containing different amino acids (i.e., substitutions or mutations) or deleting amino acids (i.e., amino acid insertions or amino acid deletions in one or both polypeptides). Sequence identity can be calculated by dividing the number of positions containing the same nucleotide or amino acid residue by the total number of amino acid residues in the polynucleotide or polypeptide. For example, percent identity can be calculated by dividing the number of positions containing the same nucleotide or amino acid residue by the total number of nucleotides or amino acid residues in the polynucleotide or polypeptide and multiplying by 100.

Illustratively, two or more sequences or subsequences have "sequence identity" or "percent identity" of at least 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% nucleotide when compared and aligned for maximum correspondence as measured using a sequence comparison algorithm or by visual inspection. In certain embodiments, the sequences are substantially identical over the entire length of either or both of the biopolymers (e.g., polynucleotides) being compared.

The term "recombinant nucleic acid molecule" refers to a polynucleotide having sequences that are not linked together in nature. The recombinant polynucleotide may be included in a suitable vector, and the vector may be used for transformation into a suitable host cell. The polynucleotides are then expressed in recombinant host cells to produce, for example, "recombinant polypeptides," "recombinant proteins," "fusion proteins," and the like.

The term "recombinant expression vector" refers to a DNA construct used to express, for example, a polynucleotide encoding a desired polypeptide. Recombinant expression vectors can include, for example, a collection comprising i) genetic elements that have a regulatory effect on gene expression, such as promoters and enhancers; ii) a structural or coding sequence that is transcribed into mRNA and translated into protein; and iii) transcriptional subunits of appropriate transcriptional and translational initiation and termination sequences. The recombinant expression vector is constructed in any suitable manner. The nature of the vector is not critical and any vector may be used, including plasmids, viruses, phages and transposons. Possible vectors for use in the present disclosure include, but are not limited to, chromosomal, non-chromosomal and synthetic DNA sequences, such as viral plasmids, bacterial plasmids, phage DNA, yeast plasmids, and vectors derived from combinations of plasmids and phage DNA, DNA from viruses such as lentiviruses, retroviruses, vaccinia, adenovirus, fowlpox, baculovirus, SV40 and pseudorabies.

The term "host cell" refers to a cell into which an exogenous polynucleotide has been introduced, including progeny of such a cell. Host cells include "transformants" and "transformed cells," which include the primary transformed cell and progeny derived therefrom. Host cells are any type of cell system that can be used to produce the antibody molecules of the invention, including eukaryotic cells, e.g., mammalian cells, insect cells, yeast cells; and prokaryotic cells, e.g., E.coli cells. Host cells include cultured cells, and also include cells within transgenic animals, transgenic plants, or cultured plant tissues or animal tissues. The term "recombinant host cell" encompasses host cells which differ from the parent cell after introduction of a circular nucleic acid molecule, a circularized precursor nucleic acid molecule, a recombinant nucleic acid molecule or a recombinant expression vector, the recombinant host cell being in particular realized by transformation. The host cell of the present disclosure may be a prokaryotic cell or a eukaryotic cell, as long as the cell can be introduced with the circular nucleic acid molecule, the circularized precursor nucleic acid molecule, the recombinant nucleic acid molecule or the recombinant expression vector of the present disclosure.

The term "high stringency conditions" means, for probes of at least 100 nucleotides in length, prehybridization and hybridization at 42 ℃ in 5X SSPE (saline sodium phosphate EDTA), 0.3% sds, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide, following standard southern blotting procedures for 12 to 24 hours. Finally, the carrier material is washed three times, each for 15 minutes, at 65 ℃ using 2 XSSC, 0.2% SDS.

As used in this disclosure, the term "very high stringency conditions" means for probes of at least 100 nucleotides in length, prehybridization and hybridization at 42 ℃ in 5X SSPE (saline sodium phosphate EDTA), 0.3% sds, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide, following standard southern blotting procedures for 12 to 24 hours. Finally, the carrier material is washed three times, each for 15 minutes, at 70 ℃ using 2 XSSC, 0.2% SDS.

Unless defined otherwise or clearly indicated by the background, all technical and scientific terms used in this disclosure have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs.

Technical scheme

In the technical scheme of the disclosure, the meanings represented by the numbers of the nucleotide and amino acid sequence tables in the specification are as follows:

the sequences shown in SEQ ID NO 1-548 and 562-564 are polynucleotide sequences having the translation activity of the initial circular nucleic acid molecule;

the sequence shown in SEQ ID NO. 549 is a nucleotide sequence of 5' spacer sequence 1;

550 is a nucleotide sequence of 5' spacer sequence 2;

the sequence shown as SEQ ID NO 551 is a nucleotide sequence of a 3' spacer sequence 1;

552 is the nucleotide sequence of the 3' spacer sequence 2;

the sequence shown in SEQ ID NO. 553 is the nucleotide sequence of the 3' spacer sequence 3;

the sequence shown as SEQ ID NO. 554 is the nucleotide sequence of exon original 1 (E1) of the class I PIE system;

the sequence shown as SEQ ID NO. 555 is the nucleotide sequence of exon element 2 (E2) of the class I PIE system;

556 is the nucleotide sequence of the 5' intron of the class I PIE system;

the sequence shown in SEQ ID NO. 557 is the nucleotide sequence of the 3' intron of the class I PIE system;

558 is the nucleotide sequence of 5' homology arm sequence 1 (H1);

the sequence shown in SEQ ID NO. 559 is a nucleotide sequence of 5' homology arm sequence 2 (H2);

560 is the nucleotide sequence of 3' homology arm sequence 1;

the sequence shown in SEQ ID NO. 561 is a nucleotide sequence of 3' homology arm sequence 2.

Method for screening IRES based on Levenstan distance

The present disclosure is a method of screening IRES based on levenstein distance, comprising the steps of:

(2) Respectively carrying out One-Hot coding on the sample sequence and the sequence to be predicted, and classifying variables into A, T, C and G;

(4) Calculating the mean value of the Levenstan distances between all the sample sequences and the sequence to be predicted;

The screening method provided by the disclosure realizes screening and judgment of the IRES for a large number of sequence samples to be predicted by utilizing the Levenstein distance for the first time, is beneficial to researchers to selectively carry out experimental verification on the sequences to be predicted with the IRES with higher probability, and effectively saves the time and cost for screening the IRES sequences. Compared with the existing IRES prediction method, the screening method disclosed by the disclosure has the advantages of accurate result and high efficiency.

In some embodiments, in the step (5), if the average value is not less than the set prediction threshold, it is determined that the sequence to be predicted contains the IRES, otherwise, it is determined that the sequence to be predicted does not contain the IRES.

In some specific embodiments, the prediction threshold is not less than 0.5. When the prediction threshold is not less than 0.5, there is a higher probability that the IRES is included in the sequence to be predicted. In some preferred embodiments, the prediction threshold is 0.75. When the prediction threshold is 0.75, an IRES is commonly included in the sequence to be predicted.

In some embodiments, the levenstein distance is calculated by: levenstein distance between two strings a, b, levab (i, j) = max (i, j) if min (i, j) =0; levab (i, j) = min (levab (i-1, j) +1, levab (i, j-1) +1, levab (i-1, j-1) + 1) (ai! = bj), where ai! = bj is an indication function when ai! = bj is 1, otherwise 0. Note that in min terms: the first part corresponds to a delete operation (from a to b), the second part corresponds to an insert operation, and the third part corresponds to a replace operation.

In some embodiments, the method further comprises the steps of: predicting and judging the secondary structure of the sequence to be predicted containing the IRES, and combining the longest common substring to judge the position of the IRES in the sequence to be predicted.

Further, the predicting the secondary structure of the sequence to be predicted which is judged to include the IRES comprises: predicting the secondary structure of the sequence to be predicted which is judged to contain the IRES by using at least one of RNAfold, mfold, RNAfold and Vienna RNA software.

Combining with IRES analysis software such as RNAfold and the like, the position of the IRES in the sequence to be predicted containing the IRES can be further analyzed and positioned, and discovery of a new IRES sequence is facilitated.

In some embodiments, the method further comprises the steps of: and carrying out experimental verification on the sequence to be predicted which is judged to contain the IRES, and judging the IRES activity of the sequence to be predicted.

In some embodiments, the step of experimentally validating comprises: constructing a circular nucleic acid molecule in which a sequence to be predicted is operably linked to a nucleotide sequence encoding a fluorescent protein using a sequence to be predicted determined to comprise an IRES;

In some specific embodiments, the disclosure exemplifies the disclosed Human poliovirus 1string man gene _CDC5' UTR (sequence shown in SEQ ID NO: 564) with IRES activity as the sequence to be predicted, and the determination of whether the sequence shown in SEQ ID NO:564 comprises an IRES using the methods of the disclosure is as follows:

(1) Selecting a sample sequence: selecting a Human Coxsackievirus B3 (CVB 3) virus IRES sequence (SEQ ID NO: 562) and a Human Echovirus29strain JV-10 (E29) virus IRES sequence (SEQ ID NO: 563) which have high activity and are verified by experiments as sample sequences;

(2) One-Hot encoding: determining objects to be coded as a sample sequence and a sequence to be predicted as shown in the following Table1-3, and classifying variables A, T, C and G; each sample has 4 features and is converted into binary vector representation, so that the sequence letter information is converted into digital information;

TABLE1 (SEQ ID NO: 562)

	T	T	A	A	A	A	C	A	G	…	T	A	C	A	G	C	A	A	A
																				A
	0	0	1	1	1	1	0	1	0	…	0	1	0	1	0	0	1	1	1
																				T	1	1	0	0	0	0	0	0	0	…	1	0	0	0	0	0	0	0	0
C	0	0	0	0	0	0	1	0	0	…	0	0	1	0	0	1	0	0	0
																				G	0	0	0	0	0	0	0	0	1	…	0	0	0	0	1	0	0	0	0

Table 2 (SEQ ID NO: 563)

	T	T	A	A	A	A	C	A	G	…	C	A	C	C	G	C	A	A	A
																				A
	0	0	1	1	1	1	0	1	0	…	0	1	0	0	0	0	1	1	1
																				T	1	1	0	0	0	0	0	0	0	…	0	0	0	0	0	0	0	0	0
C	0	0	0	0	0	0	1	0	0	…	1	0	1	1	0	1	0	0	0
																				G	0	0	0	0	0	0	0	0	1	…	0	0	0	0	1	0	0	0	0

Table 3 (SEQ ID NO: 564)

	T	T	A	A	A	A	C	A	G	…	T	G	T	A	T	C	A	T	A
																				A
	0	0	1	1	1	1	0	1	0	…	0	0	0	1	0	0	1	0	1
																				T	1	1	0	0	0	0	0	0	0	…	1	0	1	0	1	0	0	1	0
C	0	0	0	0	0	0	1	0	0	…	0	0	0	0	0	1	0	0	0
																				G	0	0	0	0	0	0	0	0	1	…	0	1	0	0	0	0	0	0	0

(3) Traversing the sample sequences, and calculating the Levenstan distance between each sample sequence and the sequence to be predicted: wherein, a represents a sample sequence, B represents a sequence to be predicted, i and j represent rows and columns in tables 1-3 respectively, and the lewinstein distance between the sequence of Human poliovirus 1string Mahoney xu CDC 5 UTR and the sequence of Human Coxsackievirus B3 (CVB 3) virus IRES is 0.79028 and the sequence of Human poliovirus 1string Mahoney CDC 5 UTR and the sequence of Human Echoviervirus 29 string JV-10 (E29) virus IRES is 0.79380 according to the calculation formula of the lewinstein distance;

(4) The prediction threshold is set to 0.75; the calculated mean of the levenstein distances of the 2 sample sequences from the sequence to be predicted is 0.79204, which is greater than the prediction threshold of 0.75, so the sequence Human poliovirus1 strand mahoney cdc 5' utr sequence to be predicted can be judged to comprise the IRES sequence;

(5) Traversing the sample sequence, and respectively searching the longest common substring of each sample sequence and the sequence to be predicted, wherein the longest common substring of the sequence to be predicted, human poliovirus 1string Mahoney \ u CDC 5 UTR and the sequence of sample sequence Human Coxsackievirus B3 (CVB 3) virus IRES, is GCGGAACCGACTACTTTGGGTGTCCGTGTTTC, and the longest common substring of the sequence to be predicted, human poliovirus 1string Mahoney \ u CDC 5 UTR and the sequence of sample sequence Human Echovirus29strain JV-10 (E29) virus IRES is TCCTCCGGCCCTGAATGCCTAATCCCAC;

(6) Prediction of secondary structure of Human poliovirus 1string Mahoney _CDC5 'UTR sequence using RNAfold software As shown in FIG. 10, the IRES structure in the Human poliovirus 1string Mahoney _CDC5' UTR sequence can be pre-estimated to be within the oval circle marked region in conjunction with the longest common substring.

As shown in FIG. 11, the luciferase protein expression results showed that mRNA protein expression of the group consisting of Human poliovirus1strain Mahoney _CDC5' UTR was significantly higher than that of the control group consisting of Human Echovirus29strain JV-10 and Human Coxsackievirus B3. It can be seen that the method for screening IRES based on the levenstan distance provided by the present disclosure is judged to comprise the IRES to-be-predicted sequence Human poliovirus 1string mahoney _cdc5 'utr sequence, which is experimentally verified to contain IRES and can be applied to expression of circular RNA, and the Human poliovirus 1string mahoney _cdc5' utr sequence IRES activity is significantly higher than the sample sequence Human Coxsackievirus B3 (CVB 3) virus IRES sequence and Human Echovirus29 string JV-10 (E29) virus IRES sequence. Therefore, the IRES prediction method based on the Levenstan distance is high in prediction accuracy, can efficiently and accurately predict whether IRES exists in a sequence to be predicted, is high in activity of the IRES screened by the IRES prediction method, and can be applied to expression of circular RNA.

Further, 548 nucleotide sequences containing IRES are screened and found in the disclosure by the method, and in further experimental verification, the disclosure finds that any one of the nucleotide sequences of SEQ ID NO 1-548 has IRES activity and can initiate expression of a target protein in a circular nucleic acid molecule, which shows that the screening method provided by the disclosure has the advantages of high accuracy and high efficiency.

It is noted that CVB3 IRES is an IRES element that has been found to have high IRES activity and can initiate protein expression of a circular nucleic acid molecule at high levels (Wesselhoeft RA, kowalski PS, anderson DG. Engineering circular RNA for patent and stable transformation in the eukaryotic cells. Nat Commun.2018Jul 6 (1): 2629. Doi. <xnotran> , IRES CVB3 IRES , (SEQ ID NO:1, 2, 3, 4, 9, 10, 11, 13, 14, 15, 17, 18, 19, 20, 25, 26, 27, 28, 41, 42, 45, 46, 51, 56, 59, 72, 79, 91, 98, 101, 104, 106, 107, 110, 115, 116, 117, 118, 119, 122, 123, 125, 127, 129, 130, 135, 139, 165, 179, 180, 183, 186, 188, 198, 200, 215, 216, 217, 218, 219, 220, 221, 222, 223, 225, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 239, 240, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 272, 273, 274, 275, 276, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 289, 291, 293, 294, 296, 298, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 314, 315, 317, 318, 319, 321, 322, 323, 324, 326, 329, 331, 332, 333, 334, 335, 336, 348, 385, 387, 389, 392, 393, 394, 395, 406, 436, 438, 439, 441, 445, 457, 460, 496, 504, 507, 509, 511, 514, 534) mRNA CVB3 IRES, IRES , . </xnotran>

Polynucleotides having translation activity for initiating a circular nucleic acid molecule

Currently, although IRES elements with initiation of protein translation processes have been found in some species (e.g., viruses), viral IRES sequences of different species have low homology and there is currently a lack of clear criteria for determining IRES sequences. Therefore, further research and identification of IRES sequences having the activity of initiating translation of circular nucleic acid molecules is needed.

To solve the above problems, the present disclosure provides polynucleotides derived from different kinds of viruses as shown below:

Echovirus E1(strain Farouk/ATCC VR-1038)、Echovirus E2(strain USA/2013-19511)、Echovirus E3(isolate JSev001)、Echovirus E3(strain 61246-70294)、Echovirus E3(strain61247-622)、Echovirus E3(strain 61245-2710)、Echovirus E3(strain 63038-1131)、Echovirus E3(strain 63040-70881)、Echovirus E3(isolate HNWY-01)、Echovirus E3(isolate ECHO3_INMI1)、Echovirus E3(isolate Env_2016_Sep_E-3)、Echovirus E3(strain Sakhalin-11.293)、Echovirus E3(strain HAI/2016-23067A)、Echovirus E3(strain HAI/2016-23066)、Echovirus E3(strain HAI/2016-23065A)、Echovirus E3(strain HAI/2016-23061)、Echovirus E3(strain HAI/2016-23056)、Echovirus E3(strain HAI/2016-23051A)、Echovirus E3(strain HAI/2016-23050)、Echovirus E3(isolate 123-R2)、Echovirus E3(strain Sakhalin/10_DU145)、Echovirus E3(strain Sakhalin/10_RD)、Echovirus E3(isolate E3/TO/BR/018)、Echovirus E4(strain 2F5)、Echovirus 4(strain AUS250G)、Echovirus E4(strain Pesacek)、Echovirus E5、Echovirus E6、Echovirus 9(strain Barty)、Echovirus 9(strain Hill)、Echovirus E11、Echovirus E12、Echovirus E13(strain HAI/2017-23078B)、Echovirus E13(strain HAI/2016-23072)、Echovirus E13(strain HAI/2016-23073)、Echovirus E13(strain HAI/2016-23075)、Echovirus E13(strain HAI/2017-23082B)、Echovirus E14(strain RO-81-1-79)、Echovirus E14(isolate ETH_P19/E14_2016)、Echovirus E14(isolate NSW-V04-2012-ECHO14)、Echovirus E14(isolate E14/P843/2013/China)、Echovirus E14(isolate E14/P968/2013/China)、Echovirus E15(strain CH 96-51)、Echovirus E16(isolate ETH_P4/E16_2016)、Echovirus E16(isolate E16/P85/2013/China)、Echovirus E16(strain Harrington)、Echovirus 17(strain CHHE-29)、Echovirus E18(isolate PC06/JS/CHN/2019)、Echovirus E18(strain E18/JXY2-2/2019)、Echovirus E18(isolate QD9/SD/CHN/2019)、Echovirus E18(isolate LJ/0530/2019)、Echovirus E18(strain 12J3)、Echovirus E18(strain USA/2015/CA-RGDS-1049)、Echovirus E18(isolate E18-221/HeB/CHN/2015)、Echovirus E18(strain 12G5)、Echovirus E18(isolate E18-393/HeB/CHN/2015)、Echovirus E18(isolate E18-398/HeB/CHN/2015)、Echovirus E18(isolate E18-HeB15-54462/HeB/CHN/2015)、Echovirus E18(isolate E18-HeB15-54498/HeB/CHN/2015)、Echovirus E18(isolate ETH_P12/E18_2016)、Echovirus E18(isolate NSW-V13A-2008-ECHO18)、Echovirus E18(strain A83/YN/CHN/2016)、Echovirus E18(strain A86/YN/CHN/2016)、Echovirus E18(isolate Jena/ST9524/10)、Echovirus E18(isolate Jena/VI10227/10)、Echovirus E18(isolate Kor05-ECV18-054cn)、Echovirus E19(strain HAI/2016-23039B)、Echovirus E19(strain HAI/2016-23036D)、Echovirus E19(strain HAI/2016-23037D)、Echovirus E19(strain HAI/2016-23037E)、Echovirus E19(strain HAI/2016-23042B)、Echovirus E19(strain HAI/2016-23046B)、Echovirus E19(strain HAI/2016-23047)、Echovirus E19(strain HAI/2016-23054)、Echovirus E19(strain HAI/2016-23052)、Echovirus E19(strain HAI/2016-23053)、Echovirus E19(train HAI/2016-23062D)、Echovirus E19(train HAI/2016-23063B)、Echovirus E19(train HAI/2016-23064B)、Echovirus E19(strain HAI/2016-23067B)、Echovirus E19(strain HAI/2016-23070B)、Echovirus E19(strain HAI/2017-23079)、Echovirus E19(strain HAI/2017-23081A)、Echovirus E19(isolate ETH_P3/E19_2016)、Echovirus E19(strain NGR_2014)、Echovirus E19(isolate PDV_BLR_IN)、Echovirus E19(strain Burke)、Echovirus E19(strain K/542/81)、Echovirus E20(isolate E20/TO/BR/016)、Echovirus E20(strain HAI/2016-23038B)、Echovirus E20(strain HAI/2016-23041B)、Echovirus E20(strain HAI/2016-23085B)、Echovirus E20(strain HAI/2016-23065C)、Echovirus E20(strain HAI/2016-23068B)、Echovirus E20(strain HAI/2016-23069)、Echovirus E20(strain HAI/2017-23080B)、Echovirus E20(strain HAI/2017-23081B)、Echovirus E20(HAI/2016-23077B)、Echovirus E20(strain HAI/2017-23083C)、Echovirus E20(strain KM-EV20-2010)、Echovirus E20(strain JV-1)、Echovirus E21(strain 553/YN/CHN/2013)、Echovirus E21(strain Farina)、Echovirus E24(strain VEN/2018-23086)、Echovirus E24(isolate PZ18G/JS/20120703)、Echovirus E24(strain DeCamp)、Echovirus E25(strain USA/2016-19521)、Echovirus E25(strain USA/2018-23126)、Echovirus E25(strain 10-4339-2)、Echovirus E25(strain USA/CA/RGDS-2017-1010)、Echovirus E25(isolate NSW-V07-2007-ECHO25)、Echovirus E25(isolate NSW-V08-2008-ECHO25)、Echovirus E25(isolate NSW-V09-2008-ECHO25)、Echovirus E25(isolate NSW-V58-2010-ECHO25)、Echovirus E25(strain 61241-70868)、Echovirus E25(strain E25/ZE-wly/Zhejiang/CHN/2005)、Echovirus E25(isolate Jena/AN1380/10)、Echovirus E25(strain XM0297)、Echovirus E25(strain E25/2010/CHN/BJ)、Echovirus E25(isolate E25SD2010CHN)、Echovirus E25(strain HN-2)、Echovirus E25(strain JV-4)、Echovirus E26(strain Coronel)、Echovirus E27(isolate ETH_P8/E27_2016)、Echovirus E27(strain Bacon)、Echovirus E29(strain HAI/2016-23048B)、Echovirus E29(strain JV-10)、Echovirus E30(isolate E30/TO/BR/032)、Echovirus E30(isolate TL12C/NM/CHN/2016)、Echovirus E30(isolate TL7C/NM/CHN/2016)、Echovirus E30(strain USA/2018-23125)、Echovirus E30(Echo30/Hokkaido.JPN/21208/2017)、Echovirus E30(strain USA/2015/CA-RGDS-1046)、Echovirus E30(strain USA/2017/CA-RGDS-1048)、Echovirus E30(isolate B001/USA/2016)、Echovirus E30(strain 16-I10)、Echovirus E30(strain 1-B4-TW)、Echovirus E30(strain 2002-59)、Echovirus E30(strain KM/A363/09)、Echovirus E30(isolate 1-MRS2013)、Echovirus E30(isolate 3-MRS2013)、Echovirus E30(isolate 4-MRS2013)、Echovirus E30(isolate 2012EM161)、Echovirus E30(isolate E30SD2010CHN)、Echovirus E30(isolate ECV30/GX10/05)、Echovirus E30(strain Kor08-ECV30)、Echovirus E30(isolate FDJS03_84)、Echovirus 30(strain Bastianni)、Echovirus 31(strain Caldwell)、Echovirus 32(strain PR-10)、Echovirus E33(strain YNK35/CHN/2013)、Echovirus E33(strain YNA12/CHN/2013)、Human poliovirus 1(isolate CHN-Hainan/93-2)、Human poliovirus 1(isolate RUS39223)、Human poliovirus 1(isolate Pak-1)、Human poliovirus 1(isolate TJK35363 clone 6)、Human poliovirus 1(strain 3788ALB96)、Human poliovirus 1(isolate CHN15115/Xinjiang/CHN/2011)、Human poliovirus 1(isolate 29690_c1)、Human poliovirus 1(strain NIE1018316)、Human poliovirus 1(isolate EGY1218587)、Human poliovirus 1(isolate 558/BRA-PE/88)、Human poliovirus 2(isolate Env2008_E2450)、Human poliovirus 2(strain CHA1218985)、Human poliovirus 2(isolate Env2008_E3218)、Human poliovirus 2(strain MAD-2593-11)、Human poliovirus 3(strain PAK1019536)、Human poliovirus 3(isolate Env08_E2886)、Human poliovirus 3(strain SWI10947)、Human poliovirus 3(strain FIN84-2493)、Human poliovirus 3(strain USOL-D-bac)、Enterovirus A71(isolate 2019-EV-A71-R398)、Enterovirus A71(strain USA/2018-23296)、Enterovirus A71(strain 16L)、Enterovirus A76(strain 10-3291-2)、Human enterovirus A76(AY697458)、Enterovirus A89(strain KSYPH-TRMH22F/XJ/CHN/2011)、Human enterovirus A89(AY697459.1)、Enterovirus A90(strain 10-2879-1)、Enterovirus A90(isolate SCH05F/XJ/CHN/2011)、Human enterovirus A90(isolate 01336/SD/CHN/EV90)、Human enterovirus A90(AB192877.1)、Human enterovirus A90(isolate F950027)、Human enterovirus 91(AY697461.1)、Human enterovirus A92(strain RJG7)、Simian enterovirus SV19(strain NOLA-2)、Simian enterovirus SV19(isolate cg4006)、Simian enterovirus SV19(strain M19s(P2))、Simian enterovirus SV43(strain OM112t(P12))、Simian enterovirus SV46(isolate cg5400)、Simian enterovirus SV46(strain RNM5)、Enterovirus B69(strain Toluca-1)、Enterovirus B69(isolate 15_491)、Enterovirus B73(isolate 088/SD/CHN/04)、Human enterovirus B73(isolate 2776-82)、Human enterovirus 74(strain Rikaze-136/XZ/CHN/2010)、Enterovirus B75(isolateY16/XZ/CHN/2007)、Enterovirus B75(isolate 102/SD/CHN/97)、Enterovirus B75(strain USA/OK85-10362)、Human enterovirus B77(strain USA/TX97-10394)、Human enterovirus B77(strain CF496-99)、Human enterovirus B79(strain 17-2255-1_E79)、Human enterovirus B79(AB426610.1)、Human enterovirus B79(strain USA/CA79-10384)、Enterovirus B80(isolate HT-LYKH203F/XJ/CHN/2011)、Human enterovirus B80(isolate HZ01/SD/CHN/2004)、Enterovirus B81(isolate 99279/XZ/CHN/1999)、Human enterovirus B81(strain USA/CA68-10389)、Human enterovirus B82(strain USA/CA64-10390)、Human enterovirus B83(strain USA/CA76-10392)、Enterovirus B83(isolate 99245/XZ/CHN/1999)、Enterovirus B83(isolate AFP341-GD-CHN-2001)、Enterovirus B83(isolate 246/YN/CHN/08)、Enterovirus B84(strain GHA:BAR:TES/2017)、Enterovirus B84(isolate AFP452/GD/CHN/2004)、Human enterovirus B84(isolate CIV2003-10603)、Human enterovirus B85(strain HTPS-MKLH04F/XJ/CHN/2011)、Human enterovirus B85(strain BAN00-10353)、Human enterovirus B86(strain BAN00-10354)、Enterovirus B87(isolate LY02/SD/CHN/2000)、Enterovirus B88(strain 11-4644-1)、Human enterovirus B88(strain BAN01-10398)、Enterovirus B93(isolate 99052/XZ/CHN/1999)、Enterovirus B93(isolate 38-03)、Human enterovirus B97(strain 99188/SD/CHN/1999/EV97)、Human enterovirus B97(strain DT94-0227)、Human enterovirus B97(strain BAN99-10355)、Human enterovirus B98(strain:T92-1499)、Human enterovirus B100(isolate BAN2000-10500)、Human enterovirus B101(strain CIV03-10361)、Enterovirus B106(isolate AKS-AWT-AFP2F/XJ/CHN/2011)、Human enterovirus 106(isolate 148/YN/CHN/12)、Enterovirus C96(strain VEN/2018-23123A)、Enterovirus C96(isolate 127/SD/CHN/1991)、Enterovirus C96(clone V13C)、Enterovirus C99(strain 10L1)、Human enterovirus C104(isolate kvv585-16-TS)、Human enterovirus C105(strain USA/OK/2014-19362)、Human enterovirus C116(strain 126)、Enterovirus C117(strain JX-C117-40-2017)、Human enterovirus C118(isolate CQ5185)、Human enterovirus D68(strain Fermon)、Enterovirus D68(TBp-13-Ph209)、Enterovirus D70(strain JPN/1989-23292)、Enterovirus D94(strain ANG/2010-23293)、Human enterovirus D94(isolate 19/04)、Enterovirus D111(strain ANG/2010-23294)、Enterovirus D111(isolate D111-NGR-KAT-1263)、Simian enterovirus J103(isolate cg8227)、Coxsackievirus A2(isolate HN202009)、Coxsackievirus A2(isolate 16027)、Coxsackievirus A2(isolate CVA2-1388-M14/XY/CHN/2017)、Coxsackievirus A2(isolate CVA2/Shenzhen50/CHN/2012)、Coxsackievirus A2(strain 2260165)、Coxsackievirus A4(strain CA4/JX2204/2014)、Coxsackievirus A4(isolate HK458564/2016)、Coxsackievirus A5(isolate CV-A5-3487-M14-XY-CHN-2017)、Coxsackievirus A5(strain CVA5/13164/HUN/2015)、Coxsackievirus A6(isolate DN1501)、Coxsackievirus A6(strain RYN-A1205)、Coxsackievirus A7(strain MAD-3101-11)、Coxsackievirus A8(isolate 13-467/GS/CHN/2013)、Coxsackievirus A8(isolate C177/CHW/AUS/2017)、Coxsackievirus A8(isolate CV-A8/P82/2013/China)、Human coxsackievirus A8(strain Donovan)、Coxsackievirus A10(isolate TA111R)、Coxsackievirus A10(strain CA10/JX2545/2017)、Coxsackievirus A12(isolate D89)、Coxsackievirus A12(strain QD-LXH535/SD/CHN/2009)、Coxsackievirus A14(strain MAD-72-07)、Coxsackievirus A14(isolate SEN-14-254)、Human coxsackievirus A14(strain G-14)、Coxsackievirus A16(isolate AH17-18/AH/East/CHN/2017-02-12)、Coxsackievirus A16(isolate CV-A16/HVN08.039_HA_GIANGVNM/2008)、Coxsackievirus B1(strain RO-98-1-74)、Coxsackievirus B1(strain CVB1/XM0108)、Coxsackievirus B1(strain B1/Groningen/2011)、Coxsackievirus B2(strain 13-2380-2_B2)、Coxsackievirus B2(strain 14L)、Coxsackievirus B2(strain 08-749-Shimane08-JPN)、Coxsackievirus B2(strain RW41-2/YN/CHN/2012)、Coxsackievirus B2(isolate BCH314)、Coxsackievirus B3(isolate B307)、Coxsackievirus B3(isolate 2001-5)、Coxsackievirus B3(isolate DH09Y/JS/2012)、Coxsackievirus B4(isolate B401)、Coxsackievirus B4(isolate CV-B4/P11/2013/China)、Coxsackievirus B4(isolate Edwards CB4)、Coxsackievirus B5(isolate B501)、Coxsackievirus B5(strain USA/MI/2009-23030)、Coxsackievirus B6(isolate 99148/XZ/CHN/1999)、Coxsackievirus B6(strain LEV15)、Coxsackievirus A9(strain A744/YN/CHN/2009)、Coxsackievirus A9(isolate 2-MRS2013)、Coxsackievirus A1(clone V18A)、Coxsackievirus A1(isolate KS-ZPH01F/XJ/CHN/2011)、Coxsackievirus A11(isolate CV-A11_66122)、Coxsackievirus A13(clone V4B)、Coxsackievirus A13(strain BAN01-10637)、Coxsackievirus A19(strain 2019103106/XX/CHN/2019)、Coxsackievirus A19(strain 8663)、Coxsackievirus A20(strain CAM1976)、Coxsackievirus A21(isolate 12MYKLU412)、Coxsackievirus A21(strain NIV17-608-2)、Coxsackievirus A22(strain 438913)、Coxsackievirus A24(strain 20693_84_CV-A24)、Coxsackievirus A15(strain G-9)、Coxsackievirus A18(strain CAM1972)、Human rhinovirus A2(strain 12L4)、Human rhinovirus A2(strain USA/2018/CA-RGDS-1062)、Human rhinovirus A2(X02316)、Human rhinovirus A7(strain ATCC VR-1117)、Human rhinovirus A8(strain ATCC VR-1118)、Human rhinovirus A9(isolate F01)、Human rhinovirus A9(isolate F02)、Human rhinovirus A9(strain ATCC VR-489)、Human rhinovirus A10(strain ATCC VR-1120)、Human rhinovirus A11(strain RvA11/USA/2021/XHZLKL)、Human rhinovirus A11(strain SCH-107)、Human rhinovirus A11(EF173414)、Human rhinovirus A12(isolate p211)、Human rhinovirus A12(EF173415)、Human rhinovirus A13(strain ATCC VR-1123)、Human rhinovirus A13(isolate F03)、Human rhinovirus A15(isolate 7002)、Human rhinovirus A15(DQ473493)、Human rhinovirus A16(isolate KC939)、Human rhinovirus A16(HRVPP)、Human rhinovirus A18(strain HRVA18/03/ZJ/CHN/2017)、Human rhinovirus 18(strain ATCC VR-1128)、Human rhinovirus 19(strain ATCC VR-1129)、Human rhinovirus A20(strain RvA20/USA/2021/B4Q4QT)、Human rhinovirus A22(strain RvA22/USA/2021/WBLGNP)、Human Rhinovirus A23(strain RvA23/USA/2021/JZHYZ6)、Human rhinovirus A24(strain RvA24/USA/2021/QZ8RX3)、Human Rhinovirus A25(strain RvA25/USA/2021/A8F6KW)、Human Rhinovirus A28(strain RvA28/USA/2021/ADMJHA)、Human Rhinovirus A29(strain RvA29/USA/2021/273658-4)、Human rhinovirus A30(strain MCL-18-H-1135)、Human rhinovirus A31(strain RvA31/USA/2021/273760-4)、Human rhinovirus A32(strain ATCC VR-1142)、Human rhinovirus A33(strain ATCC VR-330)、Human rhinovirus A34(strain ATCC VR-1144)、Human rhinovirus A36(DQ473505.1)、Human rhinovirus A38(strain ATCC VR-1148)、Human rhinovirus A39(strain ATCC VR-340)、Human rhinovirus A40(strain 7D5)、Human rhinovirus A41(strain SC9861)、Human rhinovirus A43(strain ATCC VR-1153)、Human rhinovirus A44(DQ473499)、Human rhinovirus A45(strain ATCC VR-1155)、Human rhinovirus A46(strain RvA46/USA/2021/6EEDHN)、Human rhinovirus A47(strain ATCC VR-1157)、Human rhinovirus A49(isolate F04)、Human rhinovirus A50(strain ATCC VR-517)、Human rhinovirus A51(strain ATCC VR-1161)、Human rhinovirus A53(DQ473507)、Human rhinovirus A54(strain ATCC VR-1164)、Human rhinovirus A55(DQ473511)、Human rhinovirus A56(strain ATCC VR-1166)、Human rhinovirus A57(isolate fs ship#1-hrv-57)、Human rhinovirus A58(strain ATCC VR-1168)、Human rhinovirus A59(strain 16-J2)、Human rhinovirus A60(strain ATCC VR-1473)、Human rhinovirus A61(strain SCH-99)、Human rhinovirus A62(strain ATCC VR-1172)、Human rhinovirus A63(strain ATCC VR-1173)、Human rhinovirus A64(strain ATCC VR-1174)、Human rhinovirus A65(strain ATCC VR-1175)、Human rhinovirus A66(strain ATCC VR-1176)、Human rhinovirus A67(strain ATCC VR-1177)、Human rhinovirus A68(strain ATCC VR-1178)、Human rhinovirus A71(strain ATCC VR-1181)、Human rhinovirus A74(DQ473494)、Human rhinovirus A75(DQ473510)、Human rhinovirus A76(strain ATCC VR-1186)、Human rhinovirus A77(strain ATCC VR-1187)、Human Rhinovirus A78(strain RvA78/USA/2021/177499)、Human rhinovirus A80(strain ATCC VR-1190)、Human rhinovirus A81(isolate F06)、Human rhinovirus A82(strain ATCC VR-1192)、Human_rhinovirus A85(strain RvA85/USA/2021/AR424A)、Human rhinovirus A88(DQ473504.1)、Human rhinovirus A90(strain ATCC VR-1291)、Human rhinovirus A94(strain ATCC VR-1295)、Human rhinovirus A95(strain ATCC VR-1301)、Human rhinovirus A96(strain ATCC VR-1296)、Human rhinovirus A98(strain RvA98/USA/2021/W58KP8)、Human rhinovirus A100(strain ATCC VR-1300)、Human rhinovirus A101(strain SC1124)、Human rhinovirus A103(strain MCL-18-H-1122)、Human rhinovirus B3(NC_038312.1)、Human rhinovirus B4(DQ473490.1)、Human rhinovirus B5(strain ATCC VR-485)、Human rhinovirus B6(DQ473486.1)、Human rhinovirus B17(EF173420)、Human rhinovirus B26(strain ATCC VR-1136)、Human rhinovirus B35(strain ATCC VR-1145)、Human rhinovirus B37(EF173423)、Human rhinovirus B42(strain ATCC VR-338)、Human rhinovirus B48(DQ473488)、Human rhinovirus B52(isolate F10)、Human rhinovirus B69(strain ATCC VR-1179)、Human rhinovirus B70(DQ473489)、Human rhinovirus B72(strain ATCC VR-1182)、Human rhinovirus B79(isolate ZB/CHN/18)、Human rhinovirus B83(strain ATCC VR-1193)、Human rhinovirus B84(strain ATCC VR-1194)、Human rhinovirus B86(strain ATCC VR-1196)、Human rhinovirus B91(strain RvB91/USA/2021/95333)、Human rhinovirus B92(strain ATCC VR-1293)、Human rhinovirus B93(EF173425)、Human rhinovirus B97(strain ATCC VR-1297)、Human rhinovirus B99(strain ATCC VR-1299)、Human rhinovirus C2(isolate 470389)、Human rhinovirus C6(strain RvC6/USA/2021/LCP8K8)、Human rhinovirus C8(strain RvC8/USA/2021/7N6PM0)、Human rhinovirus C9(strain RvC9/USA/2021/96D92H)、Human rhinovirus C10(strain QCE)、Human rhinovirus C11(strain SC9849)、Human rhinovirus C12(strain RvC12/USA/2021/044858)、Human rhinovirus C15(strain RvC15/USA/2021/SUSM75)、Human rhinovirus C17(strain RvC17/USA/2021/T3RVH2)、Human rhinovirus C23(strain RvC23/USA/2021/ULVLFU)、Human rhinovirus C30(strain USA/2015/CA-RGDS-1045)、Human rhinovirus C31(strain RvC31/USA/2021/B8JUE1)、Human rhinovirus C32(strain USA/CA/RGDS-2016-1008)、Human rhinovirus C34(strain RvC34/USA/2021/BYRST7)、Human rhinovirus C35(strain RvC35/USA/2021/70881)、Human rhinovirus C36(strain RvC36/USA/2021/PEXCU4)、Human rhinovirus C39(strain RvC39/USA/2021/71206)、Human rhinovirus C40(strain RvC40/USA/2021/70389)、Human rhinovirus C41(strain USA/CA/2016-RGDS-1006)、Human rhinovirus C42(strain RvC42/USA/2021/278730)、Human rhinovirus C43(strain SC174)、Human rhinovirus C47(isolate CA-RGDS-1001)、Human rhinovirus C50(strain human/Australia/SG1/2008)、Human rhinovirus C51(isolate LZ508)、Human rhinovirus C54(isolate D3490)、Human rhinovirus C56(strain RvC56/USA/2021/466615)、Enterovirus E(isolate HeN-A2)、Enterovirus F(isolate HeN-B62)、Enterovirus G(EV-G/Pig/JPN/Kana-Uchi13/2019/G1_PL-CP)、Enterovirus I Dromedary camel enterovirus(strain 19CC)、Bovine enterovirus GX20-1、Goat enterovirus(isolate NMG-F37)、Aimelvirus 1(strain gpai001)、Ampivirus A1(strain NEWT/2013/HUN)、Equine rhinitis A virus(strain PERV-1)、Foot-and-mouth disease virus-type A(isolate A/BR19-16_08dpi_CB-RF)、Foot-and-mouth disease virus-type Asia 1(isolate Mazbi/QOL-UVAS-Pak/2006)、Foot-and-mouth disease virus-type C(isolate KEN/1/2004)、Foot-and-mouth disease virus O(isolate o6pirbright iso58)、Foot-and-mouth disease virus-type SAT 1(isolate TAN/3/80)、Duck hepatitis A virus 1(strain R85952)、Turkey avisivirus(isolate USA-IN1)、Bopivirus sp(strain bovine/TV-9682/2019-HUN)、Encephalomyocarditis virus(ZM12/14)、Human TMEV-like cardiovirus(NC_010810)、Saffold virus 3(NGT07-987)、Human cosavirus A(strain AM326/BRA-AM/2017)、Cosavirus F(strain NGR_2017_NHP_CV)、Canine picodicistrovirus(strain 209)、Equine rhinitis B virus 1、Simian hepatitis A virus、Hepatovirus D2(isolate KS111230Crimig2011)、Rodent hepatovirus(KEF121Sigmas2012)、Hepatovirus G2(isolate FO1AF48Rhilan2010)、Loch Leven virus(isolate MW12_1o)、Hunnivirus 05VZ(isolate 05VZ-75-RAT099)、Melegrivirus A(NC_023858)、Canine picornavirus、Turdivirus 3、Pasivirus A3(strain swine/Zsana1/2013/HUN)、Passerivirus(sp.strain waxbill/DB01/HUN/2014)、Wenling sharpspine skate picornavirus(strain DHBYCGS18742)、Picornaviridae(sp.rodent/RL/PicoV/FJ2015)、Avian sapelovirus、Marmot sapelovirus 2(strain HT6)、Bat picornavirus(isolate BtPV/13585-58/M.dau/DK/2014)、Bat picornavirus LMA6(isolate DesRot/Peru/LMA6_F_DrPicoV)、Sicinivirus A1(isolate JSY)、Sicinivirus A5(strain RS/BR/2015/1)、Sicinivirus(sp.isolate Environment/NLD/2019/VE_7_picorna_3)、Porcine teschovirus 10(strain Vir 460/88)、Tremovirus A(isolate GDs29)、Yili teratoscincus roborowskii picornavirus 1(strain LPWC175499)、Canine kobuvirus(US-PC0082)、Feline kobuvirus(strain FK-13)、Feline kobuvirus(strain WHJ-1)、Kobuvirus(dog/AN211D/USA/2009)、Murine kobuvirus 1(isolate MKV1/NYC/2014/M014/0146)、Kobuvirus sewage Kathmandu(isolate KoV-SewKTM)、Bovine kobuvirus(strain IL35164)、Kobuvirus cattle/Kagoshima-1-22-KoV/2014/JPN(Kagoshima-1-22-KoV/2014/JPN)、Caprine kobuvirus(isolate MN1/2018)、Ferret kobuvirus(isolate MpKoV38)、Grey squirrel kobuvirus(isolate UK 2010)、Marmot kobuvirus(strain HT9)、Ovine kobuvirus(isolate SKoV-China/SWUN/AB18/2019)、Human parechovirus type 1(PicoBank/HPeV1/a virus p123)、Human parechovirus 3(strain CAU14/2015/KR)、Human parechovirus 4(isolate K251176-02)、Human parechovirus 5(strain CT86-6760)、Human parechovirus 5(4112/SapporoC/July/2018)、Human parechovirus 6(strain:NII561-2000)、Human parechovirus 6(isolate AFW)、Human parechovirus 7、Human parechovirus 14(clone V3C)、Human parechovirus 17(isolate 157Chzj058)、Human parechovirus 18(isolate 11Chzj207)、Human parechovirus 19(isolate 67Chzj11)、Ljungan virus strain 145SL(isolate 145SLG)、Ljungan virus M1146、Ljungan virus 64-7855、Rattus tanezumi parechovirus(strain Wencheng-Rt386-3)、Parechovirus(sp.strain Parchzj-6)、Baskerville virus、Bemisia tabaci picorna-like virus 1(isolate CAU-Q1)、British Admiral virus(isolate MW13_1o)、Carfax virus、Chicken picornavirus 4(isolate 5C)、Chicken picornavirus 5(isolate 27C)、Chicken proventriculitis virus(isolate CPV/Korea/03)、Zebrafish picornavirus-1(strain NCSZCF/ZfPV/2015/North Carolina/USA)、Duck picornavirus(duck/FC22/China/2017)、Eotetranychus kankitus picorna-like virus(strain EKPLV.abc9)、Falcon picornavirus、Feline picornavirus(strain 661F)、French Guiana picornavirus(isolate French_Guiana_Picornavirus)、Leveillula taurica associated picorna-like virus 1(isolate PM-A_DN31116)、Moran virus、Mus musculus picornavirus(strain Wencheng-Mm283)、Ovine picornavirus、Pigeon mesivirus 2(strain pigeon/GALII5-PiMeV/2011/HUN)、Red-necked stint Picornavirus B-like、Sphenigellan virus、Sphenimaju virus、Washington bat picornavirus、Waterwitch virus(isolate MW03_1o)、Aphid lethal paralysis virus、Cricket paralysis virus、Drosophila C virus(strain EB)、Homalodisca coagulata virus-1、Antheraea pernyi iflavirus(isolate LnApIV-02)、Isla virus(strain Cx 1773-5)、Chaetoceros socialis f.radians RNA virus、Apple latent spherical virus。

the polynucleotide provided by the disclosure has translation activity for initiating the circular nucleic acid molecule, can mediate the expression process of the protein in the circular nucleic acid molecule, realizes efficient translation and expression of the protein, and provides a good application basis for the application of the circular nucleic acid molecule.

In some embodiments, the present disclosure provides a polynucleotide (i) having translational activity for initiating a circular nucleic acid molecule, comprising a nucleotide sequence as set forth in any one of SEQ ID NOs 1 to 548. Preferably comprising a nucleotide sequence as set forth in any one of: <xnotran> SEQ ID NO:1, 2, 3, 4, 9, 10, 11, 13, 14, 15, 17, 18, 19, 20, 25, 26, 27, 28, 41, 42, 45, 46, 51, 56, 59, 72, 79, 91, 98, 101, 104, 106, 107, 110, 115, 116, 117, 118, 119, 122, 123, 125, 127, 129, 130, 135, 139, 165, 179, 180, 183, 186, 188, 198, 200, 215, 216, 217, 218, 219, 220, 221, 222, 223, 225, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 239, 240, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 272, 273, 274, 275, 276, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 289, 291, 293, 294, 296, 298, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 314, 315, 317, 318, 319, 321, 322, 323, 324, 326, 329, 331, 332, 333, 334, 335, 336, 348, 385, 387, 389, 392, 393, 394, 395, 406, 436, 438, 439, 441, 445, 457, 460, 496, 504, 507, 509, 511, 514, 534. </xnotran>

The polynucleotide represented by any one of SEQ ID NOS 1 to 548 obtained by screening can recruit ribosomes in a circular nucleic acid molecule and initiate translation of the circular nucleic acid molecule. The level of the polynucleotide mediated circular nucleic acid molecule expression protein shown by the optimized sequence is obviously higher than that of CVB3 IRES, the expression level of target polypeptide and protein can be improved, and abundant translation initiation elements are provided for preparing protein by using circular nucleic acid molecules, serving as vaccines, producing therapeutic protein or serving as gene therapy means and the like.

Although circular nucleic acid molecules show a great application prospect in the aspects of protein expression, prevention or treatment of clinical diseases, a great deal of sequences which can be used for starting translation of circular nucleic acid molecules are not found at present. The screening method provided by the disclosure provides abundant translation initiation sequences for the circular nucleic acid molecules, and has important value for widening the application of the circular nucleic acid molecules in industry and clinic.

In some embodiments, the polynucleotide further comprises a mutant sequence of a nucleotide sequence set forth in any one of (i) (ii) having a mutated nucleotide at one or more positions corresponding to the sequence set forth in any one of (i), and the mutant sequence has translational activity to initiate the circular nucleic acid molecule.

In the present disclosure, a mutant sequence refers to a polynucleotide that comprises alterations (i.e., substitutions, insertions, and/or deletions) at one or more (e.g., several) positions relative to a "wild-type" or "compared" nucleotide sequence, wherein a substitution refers to the substitution of a nucleotide occupying a position with a different nucleotide.

In some embodiments, the mutant sequence comprises one or more nucleotides corresponding to deletions or additions to the 5' end of the nucleotide sequence set forth in any one of (i). In some embodiments, the mutant sequence comprises one or more nucleotides corresponding to deletions or additions to the 3' end of the nucleotide sequence set forth in any one of (i). In some specific embodiments, the mutant sequence comprises one or more nucleotides corresponding to internal deletions, additions and/or substitutions of the nucleotide sequence set forth in any one of (i).

In the present disclosure, the mutant sequence may have increased translational activity of the starting circular nucleic acid molecule, retained or at least partially retained translational activity of the starting circular nucleic acid molecule compared to the unmutated nucleotide sequence. In particular, it is within the scope of the disclosure that the mutated nucleotide does not result in a loss of translational activity of the starting circular nucleic acid molecule of the mutant sequence.

In some embodiments, the polynucleotide having activity of initiating translation of a circular nucleic acid molecule further comprises a nucleotide sequence that is reverse complementary to the hybridizing sequence of the nucleotide sequence set forth in (i) or (ii) and has activity of initiating translation of a circular nucleic acid molecule under high stringency hybridization conditions or very high stringency hybridization conditions.

In some embodiments, a polynucleotide having translational activity for initiating a circular nucleic acid molecule further comprises a nucleotide sequence having at least 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% sequence identity (including all ranges and percentages between these values) to a nucleotide sequence set forth in either (i) or (ii) and having translational activity for initiating a circular nucleic acid molecule.

In some embodiments, the disclosure provides polynucleotides as follows (a) ₁ )-(a ₂ ) Use in at least one of:

The polynucleotide provided by the present disclosure is used for initiating protein translation of circular nucleic acid molecules, has high translation activity, and can realize stable and efficient expression of target proteins.

Circular nucleic acid molecules

The circular nucleic acid molecule provided by the disclosure comprises a polynucleotide as shown in any sequence of (i). The circular nucleic acid molecule has high protein expression efficiency and has wide application prospect in the fields of industrial protein production, nucleic acid vaccines, expression of therapeutic proteins, gene therapy and the like.

In some embodiments, the circular nucleic acid molecule is a circular RNA molecule. More specifically, a circular nucleic acid molecule is a circular mRNA molecule that comprises a coding region encoding a polypeptide of interest. The coding region of a circular mRNA molecule is operably linked to a polynucleotide having the activity of initiating translation of the circular nucleic acid molecule, which can initiate the process of translation of the protein of the circular mRNA molecule.

In some embodiments, the circular mRNA molecule further comprises one or more of the following elements: a 5 'spacer, a 3' spacer, a second exon, a first exon.

In some preferred embodiments, the circular mRNA molecule comprises the following elements connected in sequence: a second exon E2, a 5 'spacer, a polynucleotide having translational activity to initiate a circular nucleic acid molecule, a coding region, a 3' spacer and a first exon E1. The present disclosure finds that circular mRNA molecules having this structure, upon insertion of the polynucleotides provided by the present disclosure, have increased protein expression levels.

In the present disclosure, a coding region may comprise a nucleotide sequence encoding any of the above. The sequence of the coding region is not particularly limited in this disclosure, and is set according to the kind of the target protein to be expressed.

In some specific embodiments, the 5' spacer comprises a nucleotide sequence set forth in any one of SEQ ID NOS 549-550 or a sequence having at least 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100% sequence identity to a nucleotide sequence set forth in any one of SEQ ID NOS 549-550.

In some specific embodiments, the 3' spacer comprises a nucleotide sequence as set forth in SEQ ID NOS: 551-553, or a sequence having at least 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100% sequence identity to a nucleotide sequence set forth in any one of SEQ ID NOS: 551-553.

In some specific embodiments, first exon E1 comprises the nucleotide sequence set forth as SEQ ID NO. 554, or a sequence having at least 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100% sequence identity to the nucleotide sequence set forth in SEQ ID NO. 554.

In some specific embodiments, the second exon E2 comprises the nucleotide sequence set forth in SEQ ID No. 555, or a sequence having at least 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100% sequence identity to the nucleotide sequence set forth in SEQ ID No. 555.

The nucleotide sequences of the elements can further promote the protein translation process of the circular mRNA molecule mediated by the polynucleotide and improve the activity of the polynucleotide for initiating protein translation.

In other embodiments, the circular nucleic acid molecule can also comprise other types of elements, or sequences of elements, which are not specifically limited by this disclosure, as long as the polynucleotide having the sequence shown in SEQ ID NO: 1-548 of this disclosure is capable of initiating protein translation of the circular nucleic acid molecule to achieve high levels of expression of the protein.

In some embodiments, the present disclosure provides circularized precursor nucleic acid molecules that can be circularized to form circular nucleic acid molecules as described above. Further, the circularized precursor nucleic acid molecule is a circularized precursor mRNA molecule.

In some embodiments, the circularized precursor mRNA molecule further comprises one or more of the following elements: a 5 'homology arm, a 3' intron, a second exon, a 5 'spacer, a coding region, a 3' spacer, a first exon, a 5 'intron, and a 3' homology arm.

In some specific embodiments, the circularized precursor mRNA molecule comprises the following elements in sequential linkage:

a 5 'homology arm, a 3' intron, a second exon, a 5 'spacer, a polynucleotide having translational activity to initiate a circular nucleic acid molecule, a coding region, a 3' spacer, a first exon, a 5 'intron, and a 3' homology arm.

Circularization of a precursor mRNA molecule into a loop by the following process: utilizing the ribozyme properties of the intron, the junction of the 5' intron and the first exon is cleaved upon GTP initiation; ribozyme cleavage of the first exon further attacks the junction of the 3 'intron with the second exon, causing cleavage of the junction, dissociation of the 3' intron, and ligation of the first exon and the second exon to form a circular mRNA molecule.

In some specific embodiments, the 5' homology arm comprises a sequence having at least 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100% sequence identity to the nucleotide sequence set forth in any one of SEQ ID NOs 558-559.

In some specific embodiments, the 3' homology arm comprises a sequence having at least 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100% sequence identity to the nucleotide sequence set forth in any one of SEQ ID NOS 560-561.

In some specific embodiments, the 5' intron comprises a sequence having at least 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100% sequence identity to the nucleotide sequence set forth in SEQ ID NO 556.

In some specific embodiments, the 3' intron comprises a sequence having at least 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100% sequence identity to the nucleotide sequence set forth in SEQ ID NO: 557.

In some embodiments, the present disclosure provides a recombinant nucleic acid molecule capable of being transcribed to form a circularized pre-mRNA molecule as described above. Regulatory sequences may also be included in the recombinant nucleic acid molecule in order to enable further transcription of the recombinant nucleic acid molecule into an mRNA molecule. Exemplary, the regulatory sequence is a T7 promoter linked upstream of the 5' homology arm.

In some embodiments, the present disclosure provides a recombinant expression vector comprising the recombinant nucleic acid molecule described above. The vector to which the recombinant nucleic acid molecule is ligated may be any of various vectors commonly used in the art, such as a pUC57 plasmid, and the like. The further recombinant nucleic acid molecule contains restriction enzyme cutting sites, so that the recombinant expression vector is subjected to enzyme cutting to obtain a linearized vector suitable for transcription.

In some embodiments, the present disclosure provides a recombinant host cell comprising at least one of a circular mRNA molecule, a circularized precursor mRNA molecule, a recombinant nucleic acid molecule, and a recombinant expression vector.

Examples

Other objects, features and advantages of the present disclosure will become apparent from the following detailed description. However, it should be understood that the detailed description and specific examples, while indicating specific embodiments of the disclosure, are given by way of illustration only, since various changes and modifications within the spirit and scope of the disclosure will become apparent to those skilled in the art from this detailed description.

The experimental techniques and experimental procedures used in the examples are, unless otherwise specified, conventional techniques, e.g. those not specified in the following examples, generally according to conventional conditions such as Sambrook et al, molecular cloning: the conditions described in the Laboratory Manual (New York: cold Spring Harbor Laboratory Press, 1989), or according to the manufacturer's recommendations. The materials, reagents and the like used in the examples are commercially available from normal sources unless otherwise specified.

Example 1: screening for sequences having translational activity for initiating a circular nucleic acid molecule

(1) Obtaining nucleotide sequences derived from different kinds of viruses as a set of sequences to be predicted.

(2) 583 sample IRES sequence sets that were experimentally verified to be active were downloaded from the iresite database (http:// www.

(3) one-hot coding, namely determining an object to be coded as a sequence set to be predicted obtained in the step (1) and an IRES sequence set selected in the step (2), wherein the classification variables are A, T, C and G; each sample has 4 features and is converted to a binary vector representation. The peptide is represented by SEQ ID: sequence 1 is an example, as shown in table 4 below:

TABLE 4

	T	T	A	A	A	A	C	A	G	…	C	A	C	A	T	C	A	A	A
																				A
	0	0	1	1	1	1	0	1	0	…	0	1	0	1	0	0	1	1	1
																				T	1	1	0	0	0	0	0	0	0	…	0	0	0	0	1	0	0	0	0
C	0	0	0	0	0	0	1	0	0	…	1	0	1	0	0	1	0	0	0
																				G	0	0	0	0	0	0	0	0	1	…	0	0	0	0	0	0	0	0	0

(4) And (3) calculating the Levensan distances, respectively calculating the Levensan distances between each sequence to be predicted and the selected 583 sample IRES sequences, and taking the average value of the Levensan distances. Mathematically, calculating the levenstein distance between two strings a, b, levab (i, j) = max (i, j) if min (i, j) =0; levab (i, j) = min (levab (i-1, j) +1, levab (i, j-1) +1, levab (i-1, j-1) + 1) (ai! = bj), where ai! = bj is an indicator function when ai! = bj is 1, otherwise 0. Note that in min terms: the first part corresponds to a delete operation (from a to b), the second part corresponds to an insert operation, and the third part corresponds to a replace operation. Calculating the mean of the Levenstan distances between the sequence to be predicted and the 583 sample IRES sequences, wherein the mean is up to 1.0, if the mean is more than 0.5, the sequence to be predicted is judged to possibly contain the IRES preliminarily, if the mean is more than 0.75, the sequence to be predicted is judged to possibly contain the IRES, and the Levenstan distance mean is shown in the table 5.

TABLE 5

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Example 2: verification of IRES Activity of sequences to be predicted

2.1 plasmid construction

Plasmids containing different IRES elements and the coding gene eGFP were constructed, and this step was entrusted to the biotechnology company jinsrey for gene synthesis and cloning. A DNA vector for the constructed circular RNA comprising the T7 promoter, the 5 'homology arm (SEQ ID NO: 558), the 3' intron (SEQ ID NO: 557), the second exon E2 (SEQ ID NO: 555), the 5 'spacer (SEQ ID NO: 549), the IRES element, the eGFP coding region sequence, the 3' spacer (SEQ ID NO: 551), the first exon E1 (SEQ ID NO: 554), the 5 'intron (SEQ ID NO: 556), the 3' homology arm (SEQ ID NO: 560), and the XbaI cleavage site useful for plasmid linearization was constructed. The resulting gene fragment was ligated to pUC57 vector.

2.2 Linear plasmid template preparation

2.2.1 plasmid extraction

(1) Activating externally synthesized puncture bacteria at 37 deg.c/220 rpm/3-4 hr.

(2) Taking activated bacterial liquid for amplification culture, wherein the culture conditions are as follows: 37 ℃/220 rpm/overnight.

(3) Extracting plasmid (Tiangen small quantity middle extraction kit without endotoxin), and determining OD value.

2.2.2 plasmid cleavage

The plasmid 2.2.1 is digested by a single XbaI digestion method

The enzyme digestion system is as follows:

TABLE 6

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The cleavage was carried out overnight at 37 ℃. The enzyme-cleaved products were recovered using a universal DNA gel recovery kit (Tiangen Biochemical technology Co., ltd.), the OD value was determined and the enzyme-cleaved products were identified by 1% agarose gel electrophoresis. Purified linear plasmid templates were used for in vitro transcription.

2.2.3 in vitro transcription preparation of mRNA

2.2.3.1 one-step transcriptional cyclization preparation of circular mRNA

1) An in vitro transcription reaction was performed as follows:

TABLE 7

Reagent	Volume of
		10xReaction Buffer	2μl
ATP(20mM)	2μl
		CTP(20mM)	2μl
UTP(20mM)	2μl
		GTP(20mM)	2μl
Linearized DNA template	600ng
		Pyrophosphatase	2μl
RNase inhibitor	2μl
		T7 RNA Polymerase	2μl
RNA Nuclease free Water	Total 20μl

Incubate at 37 ℃ for 2-4h, add 2. Mu.l DNaseI and digest for 15min at 37 ℃.

2) Transcript mRNA purification

The transcription products obtained above were purified by a silica-membrane centrifugal column method (Thermo, geneJET RNA Purification Kit), and the OD value and the RNA size were determined by 1% denaturing agarose gel electrophoresis (FIGS. 1 to 3). The denaturing agarose gel electrophoresis patterns shown in FIGS. 1-3 show that for successful synthesis of linear mRNA and circularized RNA, the circularized mRNA population migrated faster than the linear mRNA on the gel and the bands circularized completely.

2.2.4 transfection of 293T cells with a circular mRNA encoding EGFP and determination of fluorescence intensity

2.2.4.1 cell culture: 293T in DMEM high-glucose medium containing 10% fetal bovine serum and 1% diabody, 5% CO at 37% ₂ Culturing in an incubator. Cells were subcultured every 2-3 days.

2.2.4.2 cell transfection: 293T cells at 1X 10 before transfection ⁵ One/well inoculated in 24-well plates, 5% CO at 37% ₂ Culturing in an incubator. After the cells reached 70-90% confluence, 293T cells were transfected with mRNA at 500 ng/well using Lipofectamine Messenger Max (Invitrogen) transfection reagent as follows:

1) Dilution of Messenger MAX ^TM Reagent

TABLE 8

Reagent	Volume/pore
		MEM serum-free medium	25μl
Messenger MAX ^TM Reagent	0.75μl

After dilution and mixing, the mixture was incubated at room temperature for 10min.

2) Dilution of mRNA

TABLE 9

Reagent	Volume/pore
		mRNA	500ng
MEM serum-free medium	Make up to 25. Mu.l

3) Taking the mixed and diluted Messenger MAX ^TM Reagent and mRNA (1

TABLE 10

Reagent	Volume/pore
		Diluted Messenger MAX ^TM Reagent	25μl
Diluted mRNA	25μl

After dilution and mixing, incubation was performed for 5min at room temperature.

2) Sucking the above mixed solution 50 μ l, slowly adding into 24-well plate, 37 deg.C, 5% CO ₂ And (5) incubation and culture in an incubator.

2.2.4.2 protein expression assay

1) And (3) observing cell fluorescence: 293T cells 24h after transfection were observed for EGFP expression under a fluorescent microscope.

2) Flow cytometry detection of cell mean fluorescence intensity: 293T cells 24h after transfection were examined for mean fluorescence intensity by flow cytometry.

2.2.5 analysis of the results

The circular mRNA molecule of control 1 had NO active IRES sequence added thereto, and the circular mRNA molecule of control 2 had a Coxsackievirus B3 (CVB 3) sequence (SEQ ID NO: 562) having a high IRES activity added thereto. The experimental results are shown in the table below, if the expression quantity of EGFP is more than 0 and less than or equal to 10000, the sequence to be predicted mediates the expression of the circular RNA, and the circular RNA contains the IRES sequence, and if the expression quantity of EGFP is more than 10000, the IRES activity contained in the sequence to be predicted is excellent.

TABLE 11

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As can be seen from Table 11 above, the polynucleotides of the sequences shown in SEQ ID NOS: 1 to 548 of the present disclosure all have the protein translation activity of initiating a circular mRNA molecule, and can be used as IRES elements for constructing circular mRNA molecules having the protein and polypeptide translation activities. In some preferred embodiments, the expression level of EGFP expressed by the circular mRNA molecule constructed by the polynucleotide of the present disclosure exceeds that of the circular nucleic acid molecule constructed by Coxsackievirus B3 (CVB 3) (SEQ ID NO: 562), which indicates that the polynucleotide provided by the present disclosure has a higher IRES activity than the IRES sequence with high activity at present, and is of great significance for increasing the expression level of target protein and polypeptide by the circular nucleic acid molecule.

All technical features disclosed in the present specification may be combined in any combination. Each feature disclosed in this specification may be replaced by an alternative feature serving the same, equivalent, or similar purpose. Thus, unless expressly stated otherwise, each feature disclosed is one example only of a generic series of equivalent or similar features.

Furthermore, from the foregoing description, one skilled in the art can easily ascertain the essential characteristics of this invention, and without departing from the spirit and scope thereof, can make various changes to the invention to adapt it to various usages and conditions, and therefore such changes are intended to fall within the scope of the appended claims.

SEQUENCE LISTING

<110> Suzhou Keruimaide biomedical science and technology Co., ltd

<120> method for screening IRES based on the Levensan distance and polynucleotide screened thereby

<130> 6A23-2103333I-SU

<150> CN202111185073.9

<151> 2021-10-12

<160> 564

<170> PatentIn version 3.5

<210> 1

<211> 743

<212> DNA

<213> Echovirus E1 (strain Farouk / ATCC VR-1038)

<400> 1

ttaaaacagc ctgtgggttg ttcccaccca cagggcccac tgggcgttag cacactggta 60

tcacggtacc tttgtgcgcc tgttttatac tcccccccct aaggaaactt tagaagcaaa 120

gcaattgtga tcaatagtgg gtatggcaca ccagtcatat cttgatcaag cacttctgtt 180

cccccggact tagtaccaat agactgctca agcggttgaa ggggaaaacg ttcgttatcc 240

ggccaactac ttcgagaaac ctagtagcac catgaaagtt gcggagtgtt tcgctcagca 300

cttcccccgt gtagatcagg ctgatgagtc accgtattcc ccacgggcga ccgtgacggt 360

ggctgcgttg gcggcctgcc catggggtaa cccatgggac gctctaaaac agacacggtg 420

cgaagagtct attgagctag ttggtagtcc tccggcccct gaatgcggcc aatcctaact 480

gcggagcaca tactcccaat ccagggagca gtgtgtcgta atgggtaact ctgcagcgga 540

accgactact ttgggtgtcc gtgtttcctt ttattctcac attgactgct tatggtgaca 600

attgaaagat tgttaccata tagctattgg attggtcatc cggtgagcaa tagagctatt 660

gtttatcaat ttgttggatt tgtaccactc aacttttctg ttttgagaac actcaactac 720

atcttactgc taaacacatc aaa 743

<210> 2

<211> 697

<212> DNA

<213> Echovirus E2 (strain USA/2013-19511)

<400> 2

ctagcactcc ggtattgcgg tacccttgtg cgcctgtttt atcctccctc cccctaacgt 60

aatttagaag caagacttta acggtcatta ggtggctcag cacaccagcc gagtcaagac 120

caagcacttc tgttaccccg gactgagtat caataagctg ctcacgtggc tgaaagagaa 180

aacgttcgtt acccggccaa ttacttcgag aaacctagta ccaccatgga ggttgcgcag 240

cgtttcgctc cgcactaccc cagtgtagat caggtcgatg agtcaccgca ttccccacgg 300

gcgaccgtgg cggtggctgc gttggcggcc tgcccatggg gaaacctatg ggacgcttca 360

atactgacat ggtgtgaaga gtctattgag ctaattggta atcctccggc ccctgaatgc 420

ggctaatcct aactgcggag cagacaccca caagccagtg agcagtctgt cgtaacgggc 480

aactctgcag cggaaccgac tactttgggt gtccgtgttt ccttttattc cattactggc 540

tgcttatggt gacaattgag agattgttac catatagcta ttggattggc catccggtga 600

gcaacagagc acttatatat ctctttgttg gattcatacc actagacacc aggagtttta 660

cgtctttggt ttatatttta aggttgaaca cagcaaa 697

<210> 3

<211> 743

<212> DNA

<213> Echovirus E3 (isolate JSev001)

<400> 3

ttaaaacagc ctgtgggttg tacccaccca cagggcccac tgggcgccag cactctggta 60

ttacggtacc cttgtgcgcc tgttttatat cccttcccca aggtaaacgt tagaagcaat 120

gcactcccga tcaatagaag gtgcgacgcg ccagtcgtat ctcgatcaag cacttctgtt 180

tccccggacc gagtatcaat agactgctag cgcggttgaa ggagaaaacg ttcgttaccc 240

gactaactac ttcgagaagc ccagtagcgc catgaaagtt gcagagtgtt tcgctcagca 300

cttcccccgt gtagatcagg ccgatgagtc accgcaatcc ccacgggcga ccgtggcggt 360

ggctgcgttg gcggcctgcc tatggggaaa cccataggac gctcaaatac agacatggtg 420

cgaagagtct attgagctag ttagtactcc tccggcccct gaatgcggct aatcctaact 480

gcggagcaag tactcacaat ccagtgggca gcttgtcgta acgggtaact ctgcagcgga 540

accgactact ttgggtgtcc gtgtttcctt ttattcctac attggctgct tatggtgaca 600

atcaaagagt tgttaccata tagctattgg attggccatc cggtgactaa taaagcaatt 660

atatttcttt ttgttgggtt tgtacccctt aatacaacca gcttcaccac cttgtgttac 720

atcattagac tgaacaaggc aaa 743

<210> 4

<211> 740

<212> DNA

<213> Echovirus E3 (strain 61246-70294)

<400> 4

aaacagcctg tgggttgttc ccacccacag ggcccactgg gcgctagcac tctggtatca 60

tggtaccttt gtgcgcctgt tttatccacc ctccccaaaa tgcaacttag aagcataatc 120

caaacggtca ttaggtagct cagtacacca actgagccca gaccaagcac ttctgtcacc 180

ccggaccgag tatcaatagg ctgctcgcgc ggctgaagga gaaaacgttc gttatccggc 240

caattacttc gagaaaccca gtaccactat gaaagttgcg cagcgtttcg ttccgcacaa 300

ccccagtgta gatcaggtcg atgagtcacc gcgttcccca cgggcgaccg tggcggtggc 360

tgcgttggcg gcctgcccat ggggcaacct atgggacgct tcaatactga catggtgtga 420

agagtctatt gagctaattg gtagtcctcc ggcccctgaa tgcggctaat cctaactgcg 480

gagcagatgc tcacaaacca gtgggcagtc tgtcgtaatg ggtaactctg cagcggaacc 540

gactactttg ggtgtccgtg tttcctttca tttttacact ggctgcttat ggtgacaatt 600

gagagattgt taccatatag ctattggatt ggccatccgg tgactaacag agcaattgtt 660

tacctattta ttggttttgt accgttagat tacaaagttc taaagactct gaatcttatt 720

atagcactca atacagtaaa 740

<210> 5

<211> 723

<212> DNA

<213> Echovirus E3 (strain 61247-622)

<400> 5

ttcccaccca cagggcccac tgggcgctag cactctggta tcatggtacc tttgtgcgcc 60

tgttttatcc accctcccca aaatgcaact tagaagcata atccaagcgg tcattaggta 120

gctcagtaca ccaactgagc ccagaccaag cacttctgtc accccggacc gagtatcaat 180

aggctgctcg cgcggctgaa ggagaaaacg ttcgttatcc ggccaattac ttcgagaaac 240

ccagtaccac tatgaaagtt gcgcagcgtt tcgttccgca caaccccagt gtagatcagg 300

tcgatgagtc accgcgttcc ccacgggcga ccgtggcggt ggctgcgttg gcggcctgcc 360

catggggcaa cctatgggac gcttcaatac tgacatggtg tgaagagtct attgagctaa 420

ttggtagtcc tccggcccct gaatgcggct aatcctaact gcggagcaga tgctcacaaa 480

ccagtgggca gtctgtcgta atgggtaact ctgcagcgga accgactact ttgggtgtcc 540

gtgtttcctt ttatttttac actggctgct tatggtgaca attgagagat tgttaccata 600

tagctattgg attggccatc cggtgactaa cagagcaatt gtttacctat ttattggttt 660

tgtaccgtta gattacaaag ttctaaagac tctgaatctt attatagcac tcaatacagt 720

aaa 723

<210> 6

<211> 730

<212> DNA

<213> Echovirus E3 (strain 61245-2710)

<400> 6

tgggttgttc ccacccacag ggcccactgg gcgctagcac tctggtatca tggtaccttt 60

gtgcgcctgt tttatccacc ctccccaaaa tgcaacttag aagcataatc caaacggtca 120

ttaggtagct cagtacacca actgagccca gaccaagcac ttctgtcacc ccggaccgag 180

tatcaatagg ctgctcgcgc ggctgaagga gaaaacgttc gttatccggc caattacttc 240

gagaaaccca gtaccactat gaaagttgcg cagcgtttcg ttccgcacaa ccccagtgta 300

gatcaggtcg atgagtcacc gcgttcccca cgggcgaccg tggcggtggc tgcgttggcg 360

gcctgcccat ggggcaaccc atgggacgct tcaatactga catggtgtga agagtctatt 420

gagctaattg gtagtcctcc ggcccctgaa tgcggctaat cctaactgcg gagcagatgc 480

tcacaaacca gtgggcagtc tgtcgtaatg ggtaactctg cagcggaacc gactactttg 540

ggtgtccgtg tttcctttca tttttacact ggctgcttat ggtgacaatt gagagattgt 600

taccatatag ctattggatt ggccatccgg tgactaacag agcaattgtt tacctattta 660

ttggttttgt accgttagat tacaaagttc taaagactct gaatcttatt atagcactca 720

atacagtaaa 730

<210> 7

<211> 722

<212> DNA

<213> Echovirus E3 (strain 63038-1131)

<400> 7

tcccacccac agggcccact gggcgctagc actctggtat catggtacct ttgtgcgcct 60

gttttatcca ccctccccaa aatgcaactt agaagcataa tccaaacggt cattaggtag 120

ctcagtacac caactgagcc cagaccaagc acttctgtca ccccggaccg agtatcaata 180

ggctgctcgc gcggctgaag gagaaaacgt tcgttatccg gccaattact tcgagaaacc 240

cagtaccact atgaaagttg cgcagcgttt cgttccgcac aaccccagtg tagatcaggt 300

cgatgagtca ccgcgttccc cacgggcgac cgtggcggtg gctgcgttgg cggcctgccc 360

atggggcaac ccatgggacg cttcaatact gacatggtgt gaagagtcta ttgagctaat 420

tggtagtcct ccggcccctg aatgcggcta atcctaactg cggagcagat gctcacaaac 480

cagtgggcag tctgtcgtaa tgggtaactc tgcagcggaa ccgactactt tgggtgtccg 540

tgtttccttt catttttaca ctggctgctt atggtgacaa ttgagagatt gttaccatat 600

agctattgga ttggccatcc ggtgactaac agagcaattg tttacctatt tattggtttt 660

gtaccgttag attacaaagt tctaaagact ctgaatctta ttatagcact caatacagta 720

aa 722

<210> 8

<211> 714

<212> DNA

<213> Echovirus E3 (strain 63040-70881)

<400> 8

acagggccca ctgggcgcta gcactctggt atcatggtac ctttgtgcgc ctgttttatc 60

caccctcccc aaaatgcaac ttagaagcat aatccaaacg gtcattaggt agctcagtac 120

accaactgag cccagaccaa gcacttctgt caccccggac cgagtatcaa taggctgctc 180

gcgcggctga aggagaaaac gttcgttatc cggccaatta cttcgagaaa cccagtacca 240

ctatgaaagt tgcgcagcgt ttcgttccgc acaaccccag tgtagatcag gtcgatgagt 300

caccgcgttc cccacgggcg accgtggcgg tggctgcgtt ggcggcctgc ccatggggca 360

acccatggga cgcttcaata ctgacatggt gtgaagagtc tattgagcta attggtagtc 420

ctccggcccc tgaatgcggc taatcctaac tgcggagcag atgctcacaa accagtgggc 480

agtctgtcgt aatgggtaac tctgcagcgg aaccgactac tttgggtgtc cgtgtttcct 540

ttcattttta cactggctgc ttatggtgac aattgagaga ttgttaccat atagctattg 600

gattggccat ccggtgacta acagagcaat tgtttaccta tttattggtt ttgtaccgtt 660

agattacaaa gttctaaaga ctctgaatct tattatagca ctcaatacag taaa 714

<210> 9

<211> 682

<212> DNA

<213> Echovirus E3 (isolate HNWY-01)

<400> 9

atcacggtac ctttgtgcgc ctgttttata accctttccc caacgcaaca tagaagctat 60

acacactgat caacagcagg cacagcgcac cagttgtgtc tcgatcaagc acttctgtct 120

ccccggaccg agtatcaata gactgcttac gcggttgaag gagaaaacgt tcgttacccg 180

gctgactact tcgaaaagcc tagtagtgcc atgaaagttg cggagcgttt tgttcagcac 240

ttcccccgtg tagatcaggt cgatgagtca ctgcgttccc cacgggtgac cgtggcagtg 300

gctgcgttgg cggcctgccc gtggggcaac ccatgggacg cttcaataca aacatggtgc 360

gaagagtcta ttgagctagt cagtagtcct ccggcccctg aatgcggcta atcccaactg 420

cggagcatgc gccttcgacc cagagggtag catgtcgtaa cgggcaactc tgcagcggaa 480

ccgactactt tgggtgtccg tgtttccttt tattcttata ctggctgctt atggtgacaa 540

ttgagagatt gttaccatat agctattgga ttggccatcc ggtgagtaat agagctatta 600

tatactcctt tgtgggtttt gtgccactgg atcacaaggt cttgaaaact ctcactttta 660

tcacaacact taacactgca aa 682

<210> 10

<211> 744

<212> DNA

<213> Echovirus E3 (isolate ECHO3_INMI1)

<400> 10

ttaaaacaag gctgtgggtt gtacccgccc acagggccca ctgggcgcca gcactctggt 60

attacggtac ccttgtgcgc ctgttttata tcccttcccc aaggtaaacg ttagaagcaa 120

tgcactcccg atcaatagaa ggtgcgacgc gccagtcgta tctcgatcaa gcacttctgt 180

ttccccggac cgagtatcaa tagactgcta gcgcggttga aggagaaaac gttcgttacc 240

cgactaacta cttcgagaag cccagtagcg ccatgaaagt tgcagagtgt ttcgctcagc 300

acttcccccg tgtagatcag gccgatgagt caccgcaatc cccacgggcg accgtggcgg 360

tggctgcgtt ggcggcctgc ctatggggaa acccatagga cgctcaaata cagacatggt 420

gcgaagagtc tattgagcta gttagtactc ctccggcccc tgaatgcggc taatcctaac 480

tgcggagcaa gtactcacaa tccagtgggc agcttgtcgt aacgggtaac tctgcagcgg 540

aaccgactac tttgggtgtc cgtgtttcct tttattccta cattggctgc ttatggtgac 600

aatcaaagag ttgttaccat atagctattg gattggccat ccggtgacta ataaagcaat 660

tatatttctt tttgttgggt ttgtacccct taatacaacc agcttcacca cattgtgtta 720

catcattaga ctgaacaagg caaa 744

<210> 11

<211> 657

<212> DNA

<213> Echovirus E3 (isolate Env_2016_Sep_E-3)

<400> 11

cacaccctcc ccaaagatgt aacttagaag cactttccaa gtggtcatta ggcagctcag 60

cacaccagct gagtccagac caagcacttc tgttaccccg gaccgagtat caataggctg 120

cttgcgcggc tgaaagagaa aacgttcgtt acccggccaa ttacttcgag aaacctagta 180

ccaccatgaa agttgcgcgg cgtttcgttc cgcacaaccc cagtgtagat caggtcgatg 240

agtcaccgcg ttcctcacgg gcgaccgtgg cggtggctgc gctggcggcc tgcccatggg 300

acaacccatg ggacgcttca atgctgacat ggtgtgaaga gtctattgag ctaattggta 360

gtcctccggc ccctgaatgc ggctaatcct aactgcggag cagataccca caaaccagtg 420

ggcagtctgt cgtaacgggt aactctgcag cggaaccgac tactttgggt gtccgtgttt 480

ccttttattc atacattggc tgcttatggt gacaattgag agattgttac catatagcta 540

ttggattggc catccggtgt ctaacagagc aattgtatac ctatttatag gttttatacc 600

attgaattac aaagtcttag agactctgaa tcttattata acacttaaca cagcaaa 657

<210> 12

<211> 742

<212> DNA

<213> Echovirus E3 (strain Sakhalin-11.293)

<400> 12

ttaaaacagc tctggggttg tacccactca cagggcccac tgggcgctag cacactggta 60

tcacggtacc tttgtgcgcc tgttttataa cccctttccc aaacgcaacg tagaagccac 120

acacactgat cagtagtagg cacagcgcac cagttgtgtc tcgatcaagc acttctgtct 180

ccccggaccg agtatcaata gactgctcac gcggttgaag gagaaaacgt tcgttaaccg 240

gctgactact tcgagaaacc tagtagtact atgaaagttg cgaagcgttt cactcagcac 300

ttcccccgtg tagatcaggc cgatgagtca ctgcgttccc cacgggtgac cgtggcagtg 360

gctgcgttgg cggcctgccc atggggtaac ccatgggacg cttcaatacg gacatggtgc 420

gaagagtcta ttgagctagc cagtagtcct ccggcccctg aatgcggcta atcctaactg 480

cggagcatgt gccctcgatc cagagggtag catgtcgtaa cgggcaactc tgcagcggaa 540

ccgactactt tgggtgtccg tgtttctttt tattcttata ctggctgctt atggtgacaa 600

ttgggagatt gttaccatat agctattgga ttggccaccc ggtgactaat agagctatta 660

tctactcctt tataggtttt gtgccattga actacaaggt cttaaagact ctcaacttta 720

tcatcatact taatactgca aa 742

<210> 13

<211> 687

<212> DNA

<213> Echovirus E3 (strain HAI/2016-23067A)

<400> 13

ggtatcacgg tacctttgtg cgcctgtttt ataacccctc ccccgaatgt aacgtagaag 60

ctgaacctaa gtggtcaata ggcggctcag aacaccatct gggtcttgac caagcacttc 120

tgttaccccg gactgagtat caataagctg ctcacgcggc tgaaggagaa aacgtccgtt 180

acccggccaa ttacttcaag aaacccagta ccaccatgaa ggttgcgcag cgtttcgctc 240

cgcacaaccc cagtgtagat caggtcgatg agtcaccgca ttcctcacgg gcgaccgtgg 300

cggtggctgc gctggcggcc tgcccatggg gcaacccatg ggacgcttca atactgacat 360

ggtgcgaaga gtcaattgag ctaattggta gtcctccggc ccctgaatgc ggctaatcct 420

aactgcggag cagacgccca agctccagtg ggtagtctgt cgtaacgggt aactctgcag 480

cggaaccgac tactttgggt gtccgtgttt ccttttattc ttacattggc tgcttatggt 540

gacaattgag agattgttac catatagcta ttggattggc catccggtga caaatagagc 600

aattgtatat ctttttgttg gattcatacc actcggcaaa acaaatttca gaaccttgtg 660

ttatattatt agattaaata cagcaaa 687

<210> 14

<211> 725

<212> DNA

<213> Echovirus E3 (strain HAI/2016-23066)

<400> 14

tgttcccacc cacggggcct actgggcgcc agcacactgg tatcacggta cctttgtgcg 60

cctgttttat aacccctccc ccgaatgtaa cgtagaagct gaacctaagt ggtcaatagg 120

cggctcagaa caccatctgg gtcttgacca agcacttctg ttaccccgga ctgagtatca 180

ataagctgct cacgcggctg aaggagaaaa cgtccgttac ccggccaatt acttcaagaa 240

acccagtacc accatgaagg ttgcgcagcg tttcgctccg cacaacccca gtgtagatca 300

ggtcgatgag tcaccgcatt cctcacgggc gaccgtggcg gtggctgcgc tggcggcctg 360

cccatggggc aacccatggg acgcttcaat actgacatgg tgcgaagagt caattgagct 420

aattggtagt cctccggccc ctgaatgcgg ctaatcctaa ctgcggagca gacgcccaag 480

ctccagtggg tagtctgtcg taacgggtaa ctctgcagcg gaaccgacta ctttgggtgt 540

ccgtgtttcc ttttattctt acattggctg cttatggtga caattgagag attgttacca 600

tatagctatt ggattggcca tccggtgaca aatagagcaa ttgtatatct ttttgttgga 660

ttcataccac tcggcaaaac aaatttcaga accttgtgtt atattattag attaaataca 720

gcaaa 725

<210> 15

<211> 721

<212> DNA

<213> Echovirus E3 (strain HAI/2016-23065A)

<400> 15

cccacccacg gggcctactg ggcgccagca cactggtatc acggtacctt tgtgcgcctg 60

ttttataacc cctcccccga tcgtaacgta gaagctgaac ctaagcggtc aataggcggc 120

tcagaacacc atctgggtct tgaccaagca cttctgttac cccggactga gtatcaataa 180

gctgcttacg cggctgaagg agaaaacgtc cgttacccgg ccaattactt caagaaaccc 240

agtaccacca tgaaggttgc gcagcgtttc gctccgcaca accccagtgt agatcaggtc 300

gatgagtcac cgcattcctc acgggcgacc gtggcggtgg ctgcgttggc ggcctgccca 360

tggggtaacc catgggacgc ttcaatactg acatggtgcg aagagtctat tgagctaagt 420

ggtagtcctc cggcccctga atgcggctaa tcctaactgc ggagcagacg cccaagctcc 480

agtgggtagt ctgtcgtaac gggtaactct gcagcggaac cgactacttt gggtgtccgt 540

gtttcctttt attcttacat tggctgctta tggtgacaat tgagagattg ttaccatata 600

gctattggat tggccatccg gtgacaaata gagcaattat atatcttttt gttggattca 660

taccactcga caaaacaaat ttcagaacct tgtgttatat tattagatta aatacagcaa 720

a 721

<210> 16

<211> 721

<212> DNA

<213> Echovirus E3 (strain HAI/2016-23061)

<400> 16

tcccacccac agggcccata gggcgctagc actctggtat tgcggtacct ttgtgcgcct 60

gttttatatt cccaccccca atcgcaactt agaagcagta cttcacgacc aacagtgggc 120

gtgacacacc agccacgtct cggtcaagca cctctgtctc cccggactga gtatcaataa 180

gctgctcacg cggctgaggg agaaaacgtc cgttacccgg ccaattactt cgagaaacct 240

agtaccacca cgaaagttgc gcagcgtttc gatccgcaca accccagtgt agatcaggtc 300

gatgagtcac cgcattcccc acgggcgacc gtggcggtgg ctgcgttggc ggcctgccca 360

tgggagaacc cgtgggacgc ttcaatactg acatggtgtg aagagtctat tgagctaatt 420

ggtagtcctc cggcccctga atgcggctaa tcctaactgt ggagcagatt cccgcatgcc 480

agcgggtggt ctgtcgtaac gggcaactct gcagcggaac cgactacttt gggtgtccgt 540

gtttcctttt attcctatat tggctgctta tggtgacaat tgagagattg ttaccatata 600

gctattggat tggccatccg gtgacaaaca gagcaattat ctaccaattt gttggttttg 660

taccattgaa tttcaacgca ttcaaaactc ttaactttat tttgactctt aatacagcaa 720

a 721

<210> 17

<211> 733

<212> DNA

<213> Echovirus E3 (strain HAI/2016-23056)

<400> 17

ctgtgggttg ttcccaccca cggggcctac tgggcgccag cacactggta tcacggtacc 60

tttgtgcgcc tgttttacaa cccctccccc gaacgtaacg tagaagctga acctaagcgg 120

tcaataggcg gctcagaaca ccatctgggt cttgaccaag cacttctgtt gccccggact 180

gagtatcaat aagctgctca cgcggctgaa ggagaaaacg tccgttaccc ggccaattac 240

ttcaagaaac ccagtaccac catgaaggtt gcgcagcgtt tcgctccgca caaccccagt 300

gtagatcagg tcgatgagtc accgcattcc tcacgggcga ccgtggcggt ggctgcgctg 360

gcggcctgcc catggggtaa cccatgggac gcttcaatac tgacatggtg cgaagagtca 420

attgagctaa ttggtagtcc tccggcccct gaatgcggct aatcctaact gcggagcaga 480

cgcccaagct ccagtgggta gtctgtcgta acgggtaact ctgcagcgga accgactact 540

ttgggtgtcc gtgtttcctt ttattcttac attggctgct tatggtgaca attgagaggt 600

tgttaccata tagctattgg attggccatc cggtgacaaa tagagcaatt gtatatcttt 660

ttgttggatt cataccactc gacaaaacaa atttcagaac cttgtgttat atcattagat 720

taaacacagc aaa 733

<210> 18

<211> 708

<212> DNA

<213> Echovirus E3 (strain HAI/2016-23051A)

<400> 18

cccactgggc gctagcacac tggtatcccg gtacctttgt gcgcctgttt tataccccct 60

cctccaaccg taacctagaa gcagcaccat atggccaata gtagacgtgg cgcaccagcc 120

acgttttggt caagcacttc tgtttccccg ggccgagtat caataggctg ctcacgcggc 180

tgaaggagaa agcgtccgtt acccggccaa ttacttcgag aaacctagta ccaccatgga 240

agttgcgcgg cgtttcgctc cacacaaccc cagtgtagat cgggtcgatg agtcaccgca 300

tttccccacg ggtgaccgtg gcggtggctg cgttggcggc ctgcccatga ggaaactcat 360

gggacgcttc aatactgaca tggtgtgaag agtctattga gctaattggt agtcctccgg 420

cccctgaatg cggctaatcc taactgcgga gcagatgctc acaaaccagt gggtggtctg 480

tcgtaatggg caactctgca gcggaaccga ctactttggg tgtccgtgtt tccttttatt 540

ttcacactgg ctgcttatgg tgacaattga aggattgtta ccatatagct attggattgg 600

ccatccggtg acaaacagag cgattgtcta tctatttgtt ggttttgtac cattagattt 660

caaagtcctg aaaaccctca atttcgtctt aacactcaat tcggcaag 708

<210> 19

<211> 723

<212> DNA

<213> Echovirus E3 (strain HAI/2016-23050)

<400> 19

ttcccactca cagggcccac tgggcgctag cacactggta tcccggtacc tttgtgcgcc 60

tgttttatac cccctcctcc aaccgtaacc tagaagcagc accatatggc caatagtaga 120

cgtggcgcac cagccacgtt ttggtcaagc acttctgttt ccccgggccg agtatcaata 180

ggctgctcac gcggctgaag gagaaagcgt ccgttacccg gccaattact tcgagaaacc 240

tagtaccacc atggaagttg cgcggcgttt cgctccacac aaccccagtg tagatcgggt 300

cgatgagtca ccgcatttcc ccacgggtga ccgtggcggt ggctgcgttg gcggcctgcc 360

catgaggaaa ctcatgggac gcttcaatac tgacatggtg tgaagagtct attgagctaa 420

ttggtagtcc tccggcccct gaatgcggct aatcctaact gcggagcaga tgctcacaaa 480

ccagtgggtg gtctgtcgta atgggcaact ctgcagcgga accgactact ttgggtgtcc 540

gtgtttcctt ttattttcac actggctgct tatggtgaca attgaaggat tgttaccata 600

tagctattgg attggccatc cggtgacaaa cagagcgatt gtctatctat ttgttggttt 660

tgtaccatta gatttcaaag tcctgaaaac cctcaatttc gtcttaacac tcaattcggc 720

aag 723

<210> 20

<211> 741

<212> DNA

<213> Echovirus E3 (isolate 123-R2)

<400> 20

ttaaaacagc ctgtgggttg tacccactca cagggcccac tgggcgctag cacactggta 60

tcacggtacc tttgtgcgcc tgttttataa tcccttcccc accgtaacat agaagctaca 120

cacactgatc aacagcaggc acagcacacc agttgtgtct cgatcaagca cttctgtctc 180

cccggaccga gtatcaatag actgcttacg cggttgaagg agaaaacgtt cgtcacccgg 240

ctgactactt cgaaaagcct agtagtacca tgaatgttgc ggagtgtttc gctcagcact 300

tcccccgtgt agatcaggct gatgagtcac tgcattcccc acgggtgacc gtggcagtgg 360

ctgcgttggc ggcctgccca tggggcaacc catgggacgc ttcaatacag acatggtgcg 420

aagagtctat tgagctagtc agtagtcctc cggcccctga atgcggctaa tcctaactgc 480

ggagcatgcg cctccgaccc agggggtagc atgtcgtaat gggcaactct gcagcggaac 540

cgactacttt gggtgtccgt gtttcctttt attcttatat tggctgctta tggtgacaat 600

tgagagattg ttgccatata gctattggat tggccatccg gtgagtaata gagctattat 660

atactccttt gtgggttttg tgccactgga tcacaaggtc ttgaaaactc tcacttttgt 720

cataatacta aacactgcaa a 741

<210> 21

<211> 747

<212> DNA

<213> Echovirus E3 (strain Sakhalin/10_DU145)

<400> 21

tgaccttaaa acagctctgg ggttgtaccc actcacaggg cccactgggc gctagcacac 60

tggtatcacg gtacctttgc gcgcctgctt tataacccct tccccaaacg taacgtagaa 120

gctacacaca ctgatcaata gcaggcacag cgcaccagtt gtgtcccgat cgagcacttc 180

tgtctccccg gaccgagtat caatagactg ctcacgcggt tgaaggagaa aacgttcgtt 240

aaccggctga ctacttcgag aaacctagta gtactatgaa agttgcgaag cgtttcactc 300

agcacttccc ccgtgtaggt caggccgatg agtcactgcg ttccccacgg gtgatcgtgg 360

cagtggctgc gttggcggcc tgcccatggg gtaacccatg ggacgcttca atacggacat 420

ggtgcgaaga gtctattgag ctagccagta gtcctccggc ccctgaatgc ggctaatcct 480

aactgcggag catgtgccct cgatccagag ggtagcatgt cgtaacgggc aactctgcag 540

cggaaccgac tactttgggt gtccgtgttt ctttttattc ttatactggc tgcttatggt 600

gacaattggg agattgttac catatagcta ttggattggc cacccggtga ctaatagagc 660

tattatctac tcctttatag gttttgtgcc attgaactac aaggtcttaa agactctcaa 720

ctttatcatc atacttaata ctgcaaa 747

<210> 22

<211> 742

<212> DNA

<213> Echovirus E3 (strain Sakhalin/10_RD)

<400> 22

ttaaaacagc tctggggttg tacccactca cagggcccac tgggcgctag cacactggta 60

tcacggtacc tttgtgcgcc tgttttataa ccccttcccc aaacgtaacg tagaagctac 120

acacactgat caatagcagg cacagcgcac cagttgtgtc ccgatcgagc acttctgtct 180

ccccggaccg agtatcaata gactgctcac gcggttgaag gagaaaacgt tcgttaaccg 240

gctgactact tcgagaaacc tagtagtact atgaaagttg cgaagcgttt cactcagcac 300

ttcccccgtg tagatcaggc cgatgagtca ctgcgttccc cacgggtgac cgtggcagtg 360

gctgcgttgg cggcctgccc atggggtaac ccatgggacg cttcaatacg gacatggtgc 420

gaagagtcta ttgagctagc cagtagtcct ccggcccctg aatgcggcta atcctaactg 480

cggagcatgt gccctcgatc cagagggtag catgtcgtaa cgggcaactc tgcagcggaa 540

ccgactactt tgggtgtccg tgtttctttt tattcttata ctggctgctt atggtgacaa 600

ttgggagatt gttaccatat agctattgga ttggccaccc ggtgactaat agagctatta 660

tctactcctt tataggtttt gtgccattga actacaaggt cttaaagact ctcaacttta 720

tcatcatact taatactgca aa 742

<210> 23

<211> 571

<212> DNA

<213> Echovirus E3 (isolate E3/TO/BR/018)

<400> 23

cttctgtctc cccggactga gtatcaacag gctgcttgcg cggctgaagg agaaaacgtt 60

cgttaaccgg ccaattactt cgagaaacct agtaccacca tggatgttgc gcagtgtttc 120

gctccgcaca accccagtgt agatcaggtc gatgagtcac cgcaatcccc acgggcgacc 180

gtggcggtgg ctgcgctggc ggcctgccca cggggcaacc cgtgggacgc ttcaatactg 240

acatggtgcg aagagtctat tgagctaatt ggtagtcctc cggcccctga atgcggctaa 300

tcccaactgt ggagcagata cccacatacc agtgggcagt ctgtcgtaac gggcaactcc 360

gcagcggaac cgactacttt gggtgtccgt gtttcctttt acttttatat tggctgctta 420

tggtgacaat tgaaagattg ttgccatata gctattggat tggccaccca gtgagcaaca 480

gagcgataat atttctcttt gttgggttta taccactaga ttttaaaaac tacaacaccc 540

tactttacat tgtttgcttg aacacgagaa a 571

<210> 24

<211> 667

<212> DNA

<213> Echovirus E4 (strain 2F5)

<400> 24

ctgttttaaa aacccttccc ccttttgtaa caaatctaga agtaatacac accgatcaat 60

agcgggcgcg gcacaccagc tgcgtcttga tcaagcactt ctgtctcccc ggaccgagta 120

tcaatagact gctcgcgtgg ttgaaggaga aagcgtccgt tacccgacca actacttcga 180

gaaacccagt agtgctatgg aagctgcgaa gcgtttcgct cagcacaccc ccagtgtaga 240

tcaggtcgat gagtcaccgc attccccacg ggcgaccgtg gcggtggctg cgttggcggc 300

ctgcctacgg agtaacccgt aggacgcttc aatgtggaca tggtgcgaag agtcgattga 360

gctagttggt agtcctccgg cccctgaatg cggctaatcc taactgcgga gcgcgtgcct 420

tcaatccaga gggtggcgtg tcgtaacggg taactctgca gcggaaccga ctactttggg 480

tgtccgtgtt tccttttatt ttgtactggc tgcttatggt gacaattgaa ggattgttgc 540

catatagcta ttggattggc cacccggtgt ctaacagagc aattatttac ctctttgttg 600

gattcgtacc attcaataag actagttacc aaaccttgtg ttatatcctc agattaaaca 660

catcaaa 667

<210> 25

<211> 746

<212> DNA

<213> Echovirus 4 (strain AUS250G)

<400> 25

ttaaaacagc ctgtgggttg ctcccaccca cagggcccac tgggcgtcag cacactggta 60

tcaaggtacc tttgtgcgcc tgttttacaa accctccccc cccttgtaac aaacttagaa 120

gcaatgcaca ccgatcaata gcgggcgcgg cacgccagct gcgtcttgat caagcacttc 180

tgtctccccg gaccgagtat caatagactg cttgcgtggt tgaaggagaa agcgtccgtt 240

acccgaccaa ctacttcgag aagcccagta gcgccatgga agctgcgaag cgtttcgctc 300

agcacacccc cagtgtagat caggtcgatg agtcaccgca ttccccacgg gcgaccgtgg 360

cggtggctgc gttggcggcc tgcctacggg gtaacccgta ggacgcttca atgtagacat 420

ggtgcgaaga gtcgattgag ctagttggta atcctccggc ccctgaatgc ggctaatcct 480

aactgcggag cacgtgcctt caacccagag ggtggcgtgt cgtaacgggc aactctgcag 540

cggaaccgac tactttgggt gtccgtgttt ctttttattt tgtactggct gcttatggtg 600

acaattgaag gattgttacc atatagctat tggattggcc atccggtgcc taacagagca 660

attatttacc tctttgttgg gttcgtacca ttcaataagg ctaattacca aatcttgtgt 720

tacatcctca gattgaacac atcgaa 746

<210> 26

<211> 742

<212> DNA

<213> Echovirus E4 (strain Pesacek)

<400> 26

ttaaaacagc ctgtgggttg cacccaccca cagggcccat tgggcgctag caccctggta 60

ttacggtacc tttgtgcgcc tgttttatac ccccatcccc aatcgaaact tagaagcatt 120

acacactgat caataggagg cgcggcacgc cagccatgcc aagatcaagc acttctgtct 180

ccccggaccg agtatcaata gactgcttgc gcggttgaag gagaaaacgt tcgttacccg 240

gccaactact tcgagaaacc tagtaccacc atgaaagttg cggagtgttt cgctcagcac 300

taccccagtg tagatcaggc cgatgagtca ccgcgttccc cacgggtgac cgtggcggtg 360

gctgcgctgg cggcctgcct atggggcaac ccataggacg ctctaataca gacatggtgt 420

gaagagtcta ttgagctagt tggtgatcct ccggcccctg aatgcggcta atcctaactg 480

cggagcacac gctcacaagc cagtgagtgg tgtgtcgtaa tgggtaactc cgcagcggaa 540

ccgactactt tgggtgtccg tgtttccttt taacttcatt ttggctgctt atggtgacaa 600

ttaagaaatt gttaccatat agctattgga ttggccatcc ggtgactagt agagctatta 660

tatacttgtt tgttggcttt gtaccactaa actataaagt ccttagaact cttgatttta 720

tactaatttt gaataaggca aa 742

<210> 27

<211> 742

<212> DNA

<213> Echovirus E5

<400> 27

ttaaaacagc ctgtgggttg cacccaccca cagggcccac tgggcgctgg tacactggta 60

ttaaggtacc cttgtacgcc tgttttatat ccctcccccg atttgtaact tagaattaaa 120

gcaaacccga tcaatagtag gtgtggcaaa ccagccacat cttgatcaag cacttctgtt 180

tccccggacc gagtatcaat agactgctga cgcggttgaa ggagaaaacg ttcgttaccc 240

ggccaactac ttcgggaaac ctagtaccac catgaaggtt gcgcagtgtt tcgctccgca 300

caaccccagt gtagatcagg tcgatgagcc accgcattcc ccacaggcga ctgtggcggt 360

ggctgcgttg gcggcctgcc catggggtaa cccatgggac gcttcaatac tgacatggtg 420

cgaagagtcc actgagctag ttggtattcc tccggcccct gaatgcggct aatcctaact 480

gcggagcagg cacccacgag ccagtgggca gcctgtcgta atgggtaact ctgcagcgga 540

accgactact ttgggtgtcc gtgtttccat tttcttttta ttggctgctt atggtgacaa 600

tcacggaatt gttaccatat agctattgga ttggccatcc agtgactaac agagcagtaa 660

tattcctttt tgttggtttt gtaccactca ccgtcacgag cctcagaaca ctcgtattca 720

tcttaaccct taacaagata aa 742

<210> 28

<211> 742

<212> DNA

<213> Echovirus E6

<400> 28

ttaaaacagc ctgtgggttg cccccacccg cagggcccat tgggcgctag cacactggta 60

tcacggtacc tttgtgcgcc tgttttatcc tccctcccca agtgcaacgt agaagcaaga 120

cacaaaaggt caacagatag cccagcacac cagttgggtt tcgaccaagc acttctgtct 180

ccccggaccg agtatcaata agctgctcac gcggctgaag gagaaagtgt tcgttacccg 240

gctagttact tcgagaaacc tagtaccacc atgaaggttg cgcagcgttt cgctccgcac 300

aaccccagtg tagatcaggt cgatgagtca ccgcgttccc cacgggcgac cgtggcggtg 360

gctgcgttgg cggcctgccc atggggcaac ccatgggacg cttcaatact gacatggtgc 420

gaagagtcta ttgagctaac tagtagtcct ccggcccctg aatgcggata atcttaactg 480

cggagcaggt gctcacaatc cagtgggtgg cctgtcgtaa cgggcaactc tgcagcggaa 540

ccgactactt tgggtgtccg tgtttccttt tatctctttg ttggctgctt atggtgacaa 600

ttgaacgatt gttgccatat agctattgga ttggccatcc ggtgagcagc agatccatta 660

tttaccagtt tgttggattt gtaccacttg atcacatagt ctttagaaca cttgtcttta 720

ttctaaatct taacaaggca ag 742

<210> 29

<211> 740

<212> DNA

<213> Echovirus 9 (strain Barty)

<400> 29

ttaaaacagc ctgtgggttg ttcccaccca cagggcccat tgggcgctag cacactggta 60

tcacggtacc cttgtgcgcc tgttttatcc acccctcccc atcgtaacct agaagcatga 120

ctccaacggt caatagacag ctcagtaaac caattgagcc ttgaccaagc acttctgtta 180

ccccggactg agtatcaata ggctgctcac gcggccgaag gagaaaatgt tcgttacccg 240

gccaattact tcgagaaacc tagtaccacc atgaaggttg cgcagtgttt cgcttagcac 300

aaccccagtg tagatcaggc cgatgagtca ccgcattccc cacggtgacc gtggcggtgg 360

ctgcgctggc ggcctgccca tggggcaacc catgggacgc ttcaatactg acatggtgtg 420

aagagtctat tgagctaatt ggatgtctcc ggcccctgaa tacggctaat cctaatcgcg 480

gagcagatac ccacgcacca gtgggcagtc tgtcgtaacg ggcaactctg cagcggaacc 540

gactactttg ggtgtccgtg tttcctttta ttcttgtact ggctgcttat ggtgacaatt 600

gagagattgt taccatatag ctattggatt ggccatccag tgtcaaacag agcgattgtc 660

tacttgttca ttggttttat accattgagt tacaaggttc tagccactct taacttcatt 720

ttaacactca atacatcaaa 740

<210> 30

<211> 739

<212> DNA

<213> Echovirus 9 (strain Hill)

<400> 30

ttaaaacagc ctgtgggttg tacccaccca cagggcccac tgggcgccag cactctggta 60

tcgcggtacc tttgtgcgcc tgttttgttt ccctttaccc cgaatggaac ttagaagtaa 120

tgcacactga tcaatagcag gcgtggcacc agccacgtga tgatcaagca ctactgtttc 180

cccggaccga gtatcaatag actgcttgcg cggttgaagg agaaaacgtt cgttatccgg 240

ctaactactt cgagaaaccc agtaacacca tgaaggttgc aggcgatttc gctcagcaca 300

tccccagtgt agatcaggtc gatgagtcac cgcgttccca cgggcgaccg tggcggtggc 360

tgcgttggcg gcctgcctat ggggtaaccc ataggacgct ctaatacgga catggtgcga 420

agagtctatt gagctagtta gtgttctccg gcccctgaat gcggctaatc ctaactgtgg 480

agcacatgcc cctaatccaa ggggtagtgt gtcgtaacgg gcaactccgc agcggaaccg 540

actactttgg gtgtccgtgt ttccttttat tcttacattg gctgcttatg gtgacaattg 600

agagattgtt gccatatagc tattggattg gccatccggt gtctaataga gcgataatct 660

accagtttgt tggatttgta ccactcaact tttcaacact caagacactc aactacatac 720

tagcactcaa cacagtaaa 739

<210> 31

<211> 751

<212> DNA

<213> Echovirus E11

<400> 31

ttaaaacagc ctgtgggttg tacccaccca cagggcccac tgggcgctag cacactggta 60

tcacggtacc tttgtgcgcc tgttttatac ccccttcccg caaccgcaaa tttagaagca 120

aagctaaccc gatcgatagc ggatgcgcat gccagccgca ttttgatcaa gtacttctgt 180

ttccccggac cgagtatcaa tagactgctc acgcggttga aggagaaaac gtccgttacc 240

cgaccaacta cttcgagaaa cctagtaaca tcatgaatgt tgcagggcgt ttcgatcagc 300

acgaccctgg tgtagatcag gctgatgagt caccgcattc cccacgggtg accgtggcgg 360

tggctgcgtt ggcggcctgc ctatggggtg acccatagga cgctctaata cggacatggt 420

gcgaagagtc tattgagcta gttggtagtc ctccggcccc tgaatgcggt taatcctaac 480

tgcggacgac atacccctaa tccaaggggc agtgtgtcgt aacgggcaac tctgcagcgg 540

aaccgactac tttgggtgtc cgtgtttcct tttattttta tactggctgc ttatggtgac 600

aatctcagag ttgttaccat atagctattg gattggccat ccggtgagca acagagctgt 660

catttatcag tttgttggct ttatacctct aaatcacacg gttttttttt tttggaacgc 720

ttgtattcat cttaaccctc aataaggcaa a 751

<210> 32

<211> 741

<212> DNA

<213> Echovirus E12

<400> 32

ttaaaacagc ctgtgggttg tccccaccca cagggcccac tgggcgctag cacactggta 60

tcccggtacc tttgtgcgcc tgttttatat accctcccct cagtaaccta gaagttcatc 120

acaaatgatc aatagttagc tcaacaaacc agttgagcct agatcaagca cttctgttac 180

cccgggctga gtatcaataa gctgttgaca cggctgaagg agaaaacgcc cgttacccga 240

ccagctactt cggagaacct agtatcacca tagaggttgc gtagcgtttc gctccgcaca 300

accccagtgt agatcaggtc gatgagtcac cgcgttcccc acaggcgact gtggcggtgg 360

ctgcgttggc ggcctgccca tggggttacc catgggacgc ttcaatactg acatggtgtg 420

aagagttgac tgagctagct ggtagtcctc cggcccctga atgcggctaa tcctaactgt 480

ggagcaagtg cccacaaccc agtgggtggc ttgtcgtaat gggcaactct gcagcggaac 540

cgactacttt gggtgaccgt gtttctcttt attcttatat tggctgctta tggtgacaat 600

ctcagagttg ttaccatata gctattggtt tggccaacca gtgactaaca gagcaattat 660

atatcttttc attggattta tacctataaa caacaccagt tataccactt tgtgttacat 720

tattggcttg aactcgaaaa g 741

<210> 33

<211> 727

<212> DNA

<213> Echovirus E13 (strain HAI/2017-23078B)

<400> 33

gttgttccca cccgcagggc ccactgggcg ctagcacact ggtatctcgg tacctttgtg 60

cgcctgtttt atagcccctc ccccgaatgt aacgtagaag ctgaacccaa gcggtcaata 120

ggcggctcag aacaccatct gggtcttgac caagcacttc tgttaccccg gactgagtat 180

caacaagctg ctcacgcggc tgaaggagaa aacgttcgtt acccggccaa ttacttcgag 240

aagcccagta ccaccatgaa ggttgcgcag cgtttcgctc cgcacaaccc cagtgtagat 300

caggtcgatg agtcaccgcg ttcctcacgg gtgaccgtgg cggtggctgc gctggcggcc 360

tgcccatggg gtaacccatg ggacgcttca atactgacat ggtgcgaaga gtcgattgag 420

ctaattggta gtcctccggc ccctgaatgc ggctaatcct aactgcggag cagatgccca 480

agctccagtg ggtagtctgt cgtaatgggt aactctgcag cggaaccgac tactttgggt 540

gtccgtgttt ccttttattc ttacattggc tgcttatggt gacaattgag agattgttac 600

catatagcta ttggattggc catccggtga caaatagagc aattatatat ctttttgtcg 660

gattcgtacc actcgacaaa acaaatttcc aaaccttgtg ttacattatt agactaaata 720

cagcaaa 727

<210> 34

<211> 698

<212> DNA

<213> Echovirus E13 (strain HAI/2016-23072)

<400> 34

gccagcacac tggtatctcg gtacctttgt gcgcctgttt tatagcccct cccccgaatg 60

taacgtagaa gctgaaccta agtggtcaat aggcggctca gaacaccatc tgggtcttga 120

ccaagcactt ctgttacccc ggactgagta tcaataagct gctcacgcgg ctgaaggaga 180

aaacgttcgt tacccggcca attacttcga gaaacccagt atcaccatga aggttgcgca 240

gcgtttcgct ccgcacaacc ccagtgtaga tcaggtcgat gagtcaccgc gttcctcacg 300

ggcgaccgtg gcggtggctg cgctggcggc ctgcccatgg ggcaacccat gggacgcttc 360

aatgctgaca tggtgcgaag agtcaattga gctaattggt agtcctccgg cccctgaatg 420

cggctaatcc caactgcgga gcagacgccc aaactccagt gggtagtctg tcgtaacggg 480

taactctgca gcggaaccga ctactttggg tgtccgtgtt tccttttatt cttacactgg 540

ctgcttatgg tgacaattga gagattgtta ccatatagct attggattgg ccatccggtg 600

acaaatagag caattatata tctttttgtt ggattcgtac cactcgacaa aacaaatttc 660

aaaaccttgt gttacattat tagactaaat acagcaaa 698

<210> 35

<211> 722

<212> DNA

<213> Echovirus E13 (strain HAI/2016-23073)

<400> 35

tcccacccac ggggcccacc gggcgccagc acactggtat ctcggtacct ttgtgcgcct 60

gttttatagc ccctcccccg aatgtaacgt agaagctgaa cctaagtggt caataggcgg 120

ctcagaacac catctgggtc ttgaccaagc acttctgtta ccccggactg agtatcaata 180

agctgctcac gcggctgaag gagaaaacgt tcgttacccg accaattact tcgagaaacc 240

cagtatcacc atgaaggttg cgcagcgttt cgctccgcac aaccccagtg tagatcaggt 300

cgatgagtca ccgcgttcct cacgggcgac cgtggcggtg gctgcgctgg cggcctgccc 360

atggggcaac ccatgggacg cttcaatgct gacatggtgc gaagagtcaa ttgagctaat 420

tggtagtcct ccggcccctg aatgcggcta atcccaactg cggagcagac gcccaaactc 480

cagtgggtag tctgtcgtaa cgggtaactc tgcagcggaa ccgactactt tgggtgtccg 540

tgtttccttt tattcttaca ctggctgctt atggtgacaa ttgagagatt gttaccatat 600

agctattgga ttggccatcc ggtgacaaat agagcaatta tatatctttt tgttggattc 660

gtaccactcg acaaaacaaa tttcaaaacc ttgtgttaca ttattagact aaatacagca 720

aa 722

<210> 36

<211> 726

<212> DNA

<213> Echovirus E13 (strain HAI/2016-23075)

<400> 36

gtgttcccac ccacggggcc tactgggcgc cagcacactg gtatcacggt acctttgtgc 60

gcctgtttta taacccctcc cccgaatgta acgtagaagc tgaacctaag cggtcaatag 120

gcggctcaga acaccatctg ggtcttgacc aagcacttct gttaccccgg actgagtatc 180

aataagctgc ttacgcggct gaaggagaaa acgttcgtta cccggccaat tacttcaaga 240

aacccagtac caccatgaag gttgcgcagc gtttcgctcc acacaacccc agtgtagatc 300

aggtcgatga gtcaccgcgt tcctcacggg cgaccgtggc ggtggctgcg ctggcggcct 360

gcccatgggg caacccatgg gacgcttcaa tactgacatg gtgcgaagag tcaattgagc 420

taattggtag tcctccggcc cctgaatgcg gctaatccta actgcggagc agacgcccaa 480

gctccagtgg gtagtctgtc gtaacgggta actctgcagc ggaaccgact actttgggtg 540

tccgtgtttc cttttattct tacattggct gcttatggtg acaattgaga gattgttacc 600

atatagctat tggattggcc atccggtgac aaatagagca attgtatatc tttttgttgg 660

attcatatca ctcgacaaaa caaatttcag aaccttgtgt tatattatta gattaaatac 720

agcaaa 726

<210> 37

<211> 721

<212> DNA

<213> Echovirus E13 (strain HAI/2017-23082B)

<400> 37

cccacccgca gggcccaccg ggcgctagca cactggtatc tcggtacctt tgtgcgcctg 60

ttttatagcc cctcccccga atgtaacgta gaagctgaac ccaagtggtc aataggcggc 120

tcagaacacc atctgggtct tgaccaagca cttctgttac cccggactga gtatcaacaa 180

gctgctcacg cggctgaagg agaaaacgtt cgttacccgg ccaattactt cgagaagccc 240

agtaccacca tgaaggttgc gcagcgtttc gctccgcaca accccagtgt agatcaggcc 300

gatgagtcac cgcgttcctc acgggtgacc gtggcggtgg ctgcgctggc ggcctgccca 360

tggggtaacc catgggacgc ttcaatactg acatggtgcg aagagtcgat tgagctaatt 420

ggtagtcctc cggcccctga atgcggctaa tcctaactgc ggagcagatg cccaagctcc 480

agtgggtagt ctgtcgtaat gggtaactct gcagcggaac cgactacttt gggtgtccgt 540

gtttcctttt attcttacat tggctgctta tggtgacaat tgagagattg ttaccatata 600

gctattggat tggccatccg gtgacaaata gagcaattat atatcttttt gtcggattcg 660

taccactcga caaaacaaat ttccaaacct tgtgttacat tgttagacta aatacagcaa 720

a 721

<210> 38

<211> 741

<212> DNA

<213> Echovirus E14strain RO-81-1-79

<400> 38

taagtttcaa ttgggggttg tacccaccca cagggcccat tgggcgctag cactctggta 60

tcacggtacc cttgtgcgcc tgttttataa ccccccccca attgaaactt agaagttgca 120

cgcactgatc aatagcaaac atagcaaccc agctatgtca agatcaagca ctcctgtctc 180

cccggaccga gtatcaatag actgctcacg cggtcgaagg agaaagtgtt cgttacccgg 240

ctaactactt cgaaaaacct agtaacacca tgaaagttgc gaagtgtttc gctccgcact 300

tccccagtgt agatcaggcc gatgagtcac cgagtacccc acgggcgacc gtgtcggtgg 360

ctgcgttggc ggcctgccta cggggcgacc cgtaggacgc ttcaatacag acatggtgcg 420

aagagtctat tgagctagtt agtagtcctc cggcccctga atgcggctaa tcctaactgc 480

ggagcacgtg ctctcaagcc agagagtggc gtgtcgtaac gggcaactct gcagcggaac 540

cgactacttt gggtgtccgt gtttccttta tttcttactc tggctgctta tggtgacaat 600

tgagagattg ttaccatata gctattggat tggccatccg gtgagcaaca gagcgatcat 660

ataccaattt gttggcttca tcccacttga cctaaaagcc ctacagacgc taaattttat 720

tatcatttta aacaatacaa a 741

<210> 39

<211> 687

<212> DNA

<213> Echovirus E14 (isolate ETH_P19/E14_2016)

<400> 39

tggtatcacg gtacctttgt gcgcctgttt tatctacccc tccccaatgt aatttagaag 60

catatgaaaa gcggtcaata gccggctcag tatgccaact gagtcttgac caagcacttc 120

tgttaccccg gactgagtat caataagctg ctcgcgcggc tgaaggagaa aacgttcgtt 180

acccggccaa ttacttcgag aaacccagta ccaccatgga ggttgcgcgg cgtttcgctc 240

cacacaaccc cagtgtagat caggtcgatg agtcaccgcg ttccccacgg gcgaccgtgg 300

cggtggctgc gttggcggcc tgcccatggg ttagcccatg ggacgcttca atactgacat 360

ggtgcgaaga gtctattgag ctaattggta gccctccggc ccctgaatgc ggctaatcct 420

aactgcggag cagacgccta catgccagtg ggtagtctgt cgtaacgggc aactctgcag 480

cggaaccgac tactttgggt gtccgtgttt ccttttatct ttgtattggc tgcttatggt 540

gacaattgaa agattgttac catatagcta ttggattggc catccagtga caaacagagc 600

gattgtttac tcatttatag gttttgtacc acttgactac aaggccctaa tcactctcaa 660

ttatatattg atactaaata cgacaaa 687

<210> 40

<211> 276

<212> DNA

<213> Echovirus E14 (isolate NSW-V04-2012-ECHO14)

<400> 40

gctaatccta actgcggagc agatacccac acaccagtgg gcagtctgtc gtaacgggca 60

actctgcagc ggaaccgact actttgggtg tccgtgtttc cttttattca tatactggct 120

gcttatggtg acaattgaaa gattgttacc atatagctac tggattggcc atccggtgac 180

aaatagagcg gttatatatc tttttgttgg ttttgtacca ctgaacccta tagttctaaa 240

aactctcaat ctaattatat tgttcaacac agcaaa 276

<210> 41

<211> 743

<212> DNA

<213> Echovirus E14 (isolate E14/P843/2013/China)

<400> 41

ttaaaacagt ctgtgggttg ttcccactca cagggcccac gtggcgctag cacaccggta 60

tttcggtacc cttgtgcgcc tgttttatgc acccctagcc ccacgtaact tagaagggaa 120

gcaacaacga tcaacagcag gtgtggcatt ccagccacat cgtgatcaag cacttctgtc 180

tccccggact gagtatcaat aagctgctcg cgcggctgaa ggagaaaacg ttcgttaccc 240

ggccaattac ttcgagaaac ccagtaccac catggaggtt gcgcagtgtt tcgttccgca 300

caaccccagt gtagatcagg tcgatgagtc accgcatccc ccacgggcga ccgtggcggt 360

ggctgcgttg gcggcctgcc catggggtaa cccatgggac gcttcaatac tgacatggtg 420

tgaagagtct attgagctaa ttggtagtcc tccggcccct gaatgcggct aatcctaact 480

gcggagcaga tacccataaa ccaatgggca gtctgtcgta acgggcaact ctgcagcgga 540

accgactact ttgggtgtcc gtgtttcctt ttattcttta tttggctgct tatggtgaca 600

attgagagat tgttgccata tagctattgg attggccatc cggtgacaaa cagagcgatc 660

gtgtacttat ttattggttt tgtaccacgt gaatttaaag ttttacaaac cctaaatctc 720

attattggac ttaatacagc aaa 743

<210> 42

<211> 743

<212> DNA

<213> Echovirus E14 (isolate E14/P968/2013/China)

<400> 42

ttaaaacagt ctgtgggttg ttcccactca cagggcccac gtggcgctag cacaccggta 60

tttcggtacc cttgtgcgcc tgttttatgc acccctagcc ccacgtaact tagaagggaa 120

gcaacaacga tcaacagcag gtgtggcatt ccagccacat cgtgatcaag cacttctgtc 180

tccccggact gagtatcaat aagctgctcg cgcggctgaa ggagaaaacg ttcgttaccc 240

ggccaattac ttcgagaaac ccagtaccac catggaggtt gcgcagtgtt tcgttccgca 300

caaccccagt gtagatcagg tcgatgagtc accgcatccc ccacgggcga ccgtggcggt 360

ggctgcgttg gcggcctgcc catggggtaa cccatgggac gcttcaatac tgacatggtg 420

tgaagagtct attgagctaa ttggtagtcc tccggcccct gaatgcggct aatcctaact 480

gcggagcaga tacccataaa ccaatgggca gtctgtcgta acgggtaact tcgcagcgga 540

accgactact ttgggtgtcc gtgtttcctt ttattcttat attggctgct tatggtgaca 600

attgaaagat tgttgccata tttctattgg attggccatc cggtgacaaa tatagcaatt 660

gtgtacctat ttgttggttt tgtaccactg aattacaaag ttttaaaaac cctcgatttc 720

attgtcgaac ttaacacagc aaa 743

<210> 43

<211> 743

<212> DNA

<213> Echovirus E15 (strain CH 96-51)

<400> 43

ttaaaacagc ctgtgggttg tacccaccca cagggcccac tgggcgctag cactctggta 60

ttacggtacc cttgtgcgcc tgttttatat accccgcccc aagcaaacgc tagatgtaac 120

gcacttatga tcaatagcag gcgtggcact ccagccacgt tatgatcaag cacttctgtc 180

tccccggacc gagtatcaat agactgctca cgcggtcgaa ggagaaaacg ttcgttaccc 240

gaccagctac ttcgagaaac ctagtaactc catggaggtt gcagagtgtt tcgttcagca 300

cttcccccgt gtagatcagg ctgatgagtc accgcgttcc tcacgggcga ccgtggcggt 360

ggctgcgttg gcggcctgcc tatgggtata cccataggac gctctaatac tgacatggtg 420

cgaagagtct attgagctag ttggtagtcc tccggcccct gaatgcggct aatcctaact 480

gcggagcaca tacccccaac ccagggggca gtgtgtcgta acgggtaact ctgcagcgga 540

accgactact ttgggtgtcc gtgtttcctt ttattcttac attggctgct tatggtgaca 600

attgagagat tgttaccata tagctattgg attggccatc cagtgactaa cagagctatt 660

atttaccttt ttgttggctt cgtatcactt ggtttaaaag aggttagtac tttatattgc 720

attatattac taaacacgag aaa 743

<210> 44

<211> 719

<212> DNA

<213> Echovirus E16 (isolate ETH_P4/E16_2016)

<400> 44

ccacccacag ggcccatcgg gcgcatgcac actggtatca cggtaccttt gtgcgcctgt 60

tttatacacc ccttcccgat gtaacgtaga atcacaacac aaagggtcaa taggaggctc 120

agtacgccaa ctgggtcacg accaagcact tctgtcaccc cggaccgagt atcaataagc 180

tgctcgcgcg gctgaaggag aaagcgttcg ttacccggcc atttacttcg agaaacctag 240

tagcaccatg gaggttgcgc ggcgtttcgc tccacacgac cccggtgtag atcaggccga 300

tgagtcaccg cactcctcac gggcgaccgt ggcggtggct gcgctggcgg cctgcccatg 360

gggtaaccca tgggacgctt caagactgac atggtgcgaa gagtctattg agctgaatgg 420

tagtcctccg gcccctgaat gcggctaatc ctaactgcgg agcagatacc caccagccag 480

tgggcagtct gtcgtaatgg gcaactctgc agcggaaccg actactttgg gtgtccgtgt 540

ttctttttat tttcatattg gctgcttatg gtgacaattg atagattgtt gccatatagc 600

tattggattg gccatccggt gtctaataga gcaattatat tccaatttgt tggatttgta 660

ccccttaacc ttaaagtcat aagaacactt agttttgtgt tactattgaa cacattaaa 719

<210> 45

<211> 743

<212> DNA

<213> Echovirus E16 (isolate E16/P85/2013/China)

<400> 45

ttaaaacagt ctgtgggttg taccccctca cagggcccac gtggcgctag cacaccggta 60

tctcggtacc cttgtgcgcc tgttttatac accctcaccc ccatgtaact tagaaggaaa 120

gcaacaatga tcaacagcag gtgtggcatc ccagtcacat cgtgatcaag cacttctgtc 180

tccccggact gagtatcaat gggctgctcg cgcggctgaa ggagaaaacg ttcgttaccc 240

ggccaattac ttcgagaaac ccagtaacac catggaggtt gcgcagtgtt tcgctccaca 300

caaccccagt gtagatcagg tcgatgagcc accgcattcc ccacgggcga ccgtggtggt 360

ggctgcgctg gcggcctgcc catggggtaa cccatgggac gcttcaatac tgacatggtg 420

tgaagagtct attgagctaa ttggtagtcc tccggcccct gaatgcggct aatcctaact 480

gcggagcaga tactcacatg ccagtgagca gtctgtcgta acgggtgact ctgcagcgga 540

accgactact ttgggtgtcc gtgtttcctt ttattcctta tttggctgct tatggtgaca 600

attgagagat tgttgccata tagctattgg attggccatc cagtgacaaa cagagctatt 660

gtgtacctgt ttgttggttt tgtaccacta aattttaaag tcttaagaac catcaacttg 720

atcatattac tgaatacaag aaa 743

<210> 46

<211> 742

<212> DNA

<213> Echovirus E16 (strain Harrington)

<400> 46

ttaaaacagc ctgtgggttg ttcccaccca cagggcccac tgggcgcaag cacactggta 60

ttacggtacc tttgtgcgcc tgttttacat cccctctccc attataactt agaagcacat 120

acaaaacgac caataggtgg tccagcactc cagctgggct ctggtcaagt acttctgtta 180

ccccggacta agtatcaata agcggctaac gcggctgaag gagaaagcgt ccgttacccg 240

gccaactact tcgggaaatc tagtaacacc atgaaggttg cgcagtgttt cgctccgcac 300

aaacccagtg tagatcaggt cgatgagtca ccgcattccc cacaggcgac tgtggcggtg 360

gctgcgttgg cggcctgccc atggggcaac ccatgggacg cttcaatact gacatggtgc 420

gaagaatcta ctgagctagt tggtagtcct ccggcccctg aatgcggcta atcctaactg 480

cggagcacat acccctaacc caaggggcag tgtgtcgtaa cgggcaactc tgcagcggaa 540

ccgactactt tgggtgtccg tgtttccttt tattcttata ctggctgctt atggtgacaa 600

ttgagaaatt gttaccatat agcttttgga ttggccatcc agtgactaac agagctatta 660

tttacttgtt tgttggcttt gtaccactca accttagagt tttaaggact ctcaatttgg 720

ttctgctatc gaatacaaca aa 742

<210> 47

<211> 743

<212> DNA

<213> Echovirus 17 (strain CHHE-29)

<400> 47

ttaaaacagc ctgtgggttg ttcccaccca cagggcccat tgggcgctag cactctggta 60

ttgcggtacc tttgtgcgcc tgttttatat tccctccccc tgaccgtaac ttagaagtaa 120

cacacaccga tcaacagtaa gcatggcaca ccagccatgt tttgatcaag cacttctgtt 180

atcccggact gagtatcaat agactgctca cgcggttgaa ggagaaaacg ttcgttaccc 240

ggccaactac ttcgaaaaac ctagtaacac catggaagtt gcagagtgtt tcgctcagca 300

ctaccccagt gtagatcagg tcgatgagtc accgcattcc ccacgggcga ccgtggcggt 360

ggctgcgttg gcggcctgcc tatggggaaa cccataggac gctctaatac agacatggtg 420

cgaagagtct attgagctag ttggtagtcc tccggcccct gaatgcggct aatcctaact 480

gcggagcaca caccctcaag ccagggggca gtgtgtcgta acgggcaact ctgcagcgga 540

accgactact ttgggtgtcc gtgtttcccc ttactcttgt attggctgct tatggtgaca 600

attttggaat tgttgccata tagctattgg attggccatc cggtgacaaa cagagcgatt 660

gtatacctat tcgttggact tgtaccgctc aacgttacga gtctaacgac tttatactac 720

atcatctggt tgaacacagc aaa 743

<210> 48

<211> 743

<212> DNA

<213> Echovirus E18 (isolate PC06/JS/CHN/2019)

<400> 48

ataaaacagc ctgtgggttg ttcccaccca cagggcccac cgggcgctag cacactggta 60

tctcggtacc tttgtgcgcc tgttttatcc acccttttcc catttaattt agaagcacgc 120

gccaagacgg tcgataggga gcccagtaag ccaattgggc tgtgaccaag cacttctgta 180

accccgggcc gagtatcaat aagctgctcg cgcggctgaa agagaaaaag ctcgttaccc 240

ggccaattac ttcgagaaac ctagtagcat catgaaagtt gcgcagcgtt tcgctccaca 300

caaccccagt gtagatcagg ccgatgagtc accgcgttcc ccacgggtga ccgtggcggt 360

ggctgcgatg gcggcctgcc catggggcca cccatgggac gcttcaatac tgacatggtg 420

tgaagagtct attgagctaa ttggtagtcc tccggcccct gaatgcggct aatcctaact 480

gcggagcaga tgcccacgca ccagtgggaa gtctgtcgta acgggtaact ctgcagcgga 540

accgactact ttgggtgtcc gtgtttcctt ttatttctac tctggctgct tatggtgaca 600

attgaaaggt tgttaccata tagccattgg attggccatc cggtgtcaaa tagagcgatt 660

atttattcgt ttgttgggtt tgtaccactg aattacaaag ccttaaacac tcttaactac 720

attttaatcc ttaactcatc aaa 743

<210> 49

<211> 743

<212> DNA

<213> Echovirus E18 (strain E18/JXY2-2/2019)

<400> 49

ttaaaacagc ctgtgggttg ttcccaccca cagggcccac cgggcgctag cacactggta 60

tcacggtacc tttgtgcgcc tgttttatcc acccttttcc cacttaatct agaagcacgc 120

accaagacgg tcaataggga gcccagtaaa ccaattgggc tatgaccaag cacttctgta 180

accccgggcc gagtatcaat aagctgctcg cgcggctgaa ggagaaaaag ctcgttaccc 240

ggccaattac ttcgagaaac ccagtagcat catgaaagtt gcgcagcgtt tcgctccaca 300

caaccccagt gtagatcagg ccgatgagtc accgcgttcc ccacgggtga ccgtggcggt 360

ggctgcgctg gcggcctgcc catggggcca cccatgggac gcttcaatat tgacatggtg 420

tgaagagtct attgagctaa ttggtagtcc tccggcccct gaatgcggct aatcctaact 480

gcggagcaga tgcccacaca ccagtgggaa gtctgtcgta acgggtaact ctgcagcgga 540

accgactact ttgggtgtcc gtgtttcctt ttatttctac tctggctgct tatggtgaca 600

attgaaaggt tgttaccata tagctattgg attggccatc cggtgtcaaa tagagcgatt 660

atttacttgt ttgttgggtt tgtaccactg aactacaaag ttttaaacac tcttaactac 720

atcttaatcc ttaactcatc aaa 743

<210> 50

<211> 726

<212> DNA

<213> Echovirus E18 (isolate QD9/SD/CHN/2019)

<400> 50

gttttcccca ccacagggcc caccgtgcgc tagccccatg gtatctcggt acccttgtgc 60

gcctgtttta tccacccttt tcccatttaa cttagaagca cgcgccaaga cggtcaaaag 120

ggagcccagt aagccaattg ggctatgacc aagcactcct gtaaccccgg gccgagtatc 180

aataagctgc tcgcgcggct gaaggagaaa aagctcgtta cccggccaat tacttcgaga 240

aacccagtag catcatgaaa gttgcgcagc gtttcgctcc acacaacccc agtgtagatc 300

aggtcgatga gtcaccgcgt tccccacggg tgaccgtggc ggtggctgcg ctggcggcct 360

gcccatgggg ccacccatgg gacgcttcaa tactgacatg gtgtgaagag tctattgagc 420

taattggtag tcctccggcc cctgaatgcg gctaatccta actgcggagc agatgcccac 480

acaccagtgg gaagtctgtc gtaacgggta actctgcagc ggaaccgact actttgggtg 540

tccgtgtttc cttttatttc tactctggct gcttatggtg acaattgaaa ggttgttacc 600

atatagctat tggattggcc atccggtgtc acatagagcg attatttact tgtttgttgg 660

gtttatacca ctgaactata aagttttaaa tactcttaac tacattttaa tccttaactc 720

attaaa 726

<210> 51

<211> 743

<212> DNA

<213> Echovirus E18 (isolate LJ/0530/2019)

<400> 51

ttataacagc ctgtgggttg ttcccactca cagggcccac cgggcgctag cacaccggta 60

tctcggtacc tttgtgcgcc tgttttatcc acccttttcc catttaactt agaagcacgc 120

gccaagacgg tcaataggga gcccagtaaa ccaattgggc tatgaccaag cacttctgta 180

accccgggcc gagtatcaat aagctgctca cgcggctgaa ggagaaaaag ctcgttaccc 240

ggccaattac ttcgagaaac ccagtagcat catgaaagtt gcgcagcgtt tcgctccaca 300

caaccccagt gtagatcagg ccgatgagcc accgcgttcc ccacgggtga ccgtggcggt 360

ggctgcgctg gcggcctgcc catggggcca cccatgggac gcttcaatac tgacatggtg 420

tgaagagtct attgagctaa ttggtagtcc tccggcccct gaatgcggct aatcctaact 480

gcggagcaga tgcccacgca ccagtgggaa gtctgtcgta acgggtaact ctgcagcgga 540

accgactact ttgggtgtcc gtgtttcctt ttatttttac tctggctgct tatggtgaca 600

attgaaaggt tgttaccata tagctattgg attggccatc cggtgtcaaa tagagcgatt 660

atttacttgt ttgttgggtt tgtgccactg aactacaaag ttttgaacac tcttaactac 720

attctaatcc ttaacttatt aaa 743

<210> 52

<211> 744

<212> DNA

<213> Echovirus E18 (strain 12J3)

<400> 52

ttaaaacagc ctgtgggttg ttcccaccca cagggcccac agggcgccag cacactggta 60

tcacggtacc tttgtgcgcc tgttttatac accctcaccc ccgatgtaac ttagaagcac 120

aactaaagcg gtcaacagaa agctcagtaa tccaactgag cgccgaccaa gcacttctgt 180

taccccggac cgagtatcaa taagctgctc gcgcggctga aggagaaaac gttcgttacc 240

cggccaatta cttcgagaaa cctagtaaca ccatgaaagt tgcgtagtgt ttcgctccgc 300

acaaccccag tgtagatcag gtcgatgagt caccgcaatc cccacgggcg accgtggcgg 360

tggctgcgtt ggcggcctgc ccatggggca acccatggga cgcttcaata ctgacatggt 420

gtgaagagtc tattgagcta attggtagtc ctccggcccc tgaatgcggc taatcccaac 480

tgcggagcag acgctcgcat gccagcgagt agtctgtcgt aacgggcaac tctgcagcgg 540

aaccgactac tttgggtgtc cgtgtttccc tttattttta tattggctac ttatggtgac 600

aattgaaaga ttgttaccat atagctattg gattggccat ccggtgacaa acagagcgat 660

catctactct tttgttgggt tcgttccatt gaatcacgag gtattgaaca cactcaattt 720

cattttaacc cttaatacac taag 744

<210> 53

<211> 741

<212> DNA

<213> Echovirus E18 (strain USA/2015/CA-RGDS-1049)

<400> 53

ttaaaacagc ctgtgggttg ctcccaccca cagggcccaa tgggcgctag cacactagta 60

ttacggtact tttgtgcgcc tgttttatcc ctcacccccg atgtaactta gaagcataat 120

taaagcggtc aatagaaagc tcagtaatcc aactgagtgc cgaccaagca cttctgttac 180

cccggaccga gtatcaataa gctgttcgcg cggctgaagg agaaaacgtt cgttacccgg 240

ccaattactt cgagaaacct agtaacacca tgaaagttgc gtagtgtttc gctccgcaca 300

accccagtgt agatcaggtc gatgagtcac cgcaatcccc acgggcgacc gtggcggtgg 360

ctgcgttggc ggcctgccca tggggcaacc catgggacgc ttcaatactg acatggtgtg 420

aagagtctat tgagctaatt ggtagtcctc cggcccctga atgcggctaa tcccaactgc 480

ggagcagacg ctcgcatgcc agcgagtagt ctgtcgtaac gggcaactct gcagcggaac 540

cgactacttt gggtgtccgt gtttcccttt atttttatat tggctgctta tggtgacaat 600

tgaaagattg ttaccatata gctattggat tggccatccg gtgacaaaca gagcgatcat 660

ctactctttt gttgggttcg ttccattgaa tcacaaggta ttgaacacac tcaatttcat 720

tttaaccctt aatacactaa g 741

<210> 54

<211> 743

<212> DNA

<213> Echovirus E18 (isolate E18-221/HeB/CHN/2015)

<400> 54

ttataacagc ctgtgggttg tacccaccca cagggcccac cgggcgctag cacactggta 60

tctcggtacc tttgtgcgcc tgttttatcc gcccttctcc catttaattt agaagcacgc 120

gccaagacgg tcaataggga gcccagtaag ccaattgggc catgaccaag cacttctgta 180

accccgggcc gagtatcaat aagctgctcg cgcggctgaa ggagaaaaag ctcgttaccc 240

ggccaattac ttcgagaagc ccagtagcat catgaaagtt gcgcagtgtt tcgctccgca 300

caaccccagt gtagatcagg ccgatgagtc accgcgttcc ccacgggtga ccgtggcggt 360

ggctgcgctg gcggcctgcc catggggcca cccatgggac gcttcaatac tgacatggtg 420

tgaagagtct attgagctaa ttggtagtcc tccggcccct gaatgcggct aatcctaact 480

gcggagcaga tgcccacgca ccagtgggaa gtctgtcgta atgggtaact ctgcagcgga 540

accgactact ttgggtgtcc gtgtttcctt ttatttctac tctggctgct tatggtgaca 600

attgaaaggt tgttaccata tagctattgg attggccatc cggtgacaaa tagagcgatt 660

atttacttgt ttgttgggtt tgtgccactg aactacaaag ctttaaacac tcttaactac 720

attttaatcc ttaactcatt aaa 743

<210> 55

<211> 742

<212> DNA

<213> Echovirus E18 (strain 12G5)

<400> 55

taaaacagcc tgtgggttgt tcccacccac agggcccaca gggcgccagc acactggtat 60

cacggtacct ttgtgcgcct gttttatgca ccctcaccct caatgtaact tagaagtaca 120

gccaagcggt caatagaagc ttagtaatcc aactgagctc tgaccaagca cttctgttgc 180

cccggaccga gtatcaataa gctgctcgcg cggctgaagg agaaaacgtt cgttatccgg 240

ccaattactt cgagaaacct agtaacgcca tgaaagttgc gcagtgtttc gctccgcaca 300

accccagtgt agatcaggtc gatgagtcac cgcaaacccc acgggcgacc gtggcggtgg 360

ctgcgttggc ggcctgccca tggggcaacc catgggacgc ttcaatactg acatggtgtg 420

aagagtctat tgagctagtt ggtagtcctc cggcccctga atgcggctaa tcctaactgc 480

ggagcagacg cttgcatacc agcgagtagt ctgtcgtaac gggcaactct gcagcggaac 540

cgactacttt gggtgtccgt gtttcctttt atcttttaca ttggctgctt atggtgacaa 600

ttgaaagatt gttgccatat agctattgga ttggccatcc ggtgacaaac agagcgatca 660

tctattcttt cgttgggttc gttccattga atcacgaggt attgaataca ctcaacctca 720

ttctaaccct taatacacta aa 742

<210> 56

<211> 743

<212> DNA

<213> Echovirus E18 (isolate E18-393/HeB/CHN/2015)

<400> 56

ttataacagc ctgtgggttg tacccaccca gagggcccac cgggcgctag cacactggta 60

tctcggtacc tttgtgcgcc tgttttatcc gcccttctcc catttaattt agaagcacgc 120

gccaagacgg tcaataggga gcccagtaag ccaattgggc catgaccaag cacttctgta 180

accccgggcc gagtatcaat aagctgctcg cgcggctgaa ggagaaaaag ctcgttaccc 240

ggccaattac ttcgagaaac ccagtagcac catgaaagtt gcgcagtgtt tcgctccaca 300

caaccccagt gtagatcagg ccgatgagtc accgcgttcc ccacgggtga ccgtggcggt 360

ggctgcgctg gcggcctgcc catggggcca cccatgggac gcttcaatac tgacatggtg 420

tgaagagtct attgagctaa ttggtagtcc tccggcccct gaatgcggct aatcctaact 480

gcggagcaga tgcccacgca ccagtgggaa gtctgtcgta acgggtaact ctgcagcgga 540

accgactact ttgggtgtcc gtgtttcctt ttatttctac tctggctgct tatggtgaca 600

attgaaaggt tgttaccata tagctattgg attggccatc cggtgacaaa tagagcgatt 660

atttacttgt ttgttgggtt tgtaccactg aactacaaag ctttgaacac tctttactac 720

attttaatcc ttaactcatt gaa 743

<210> 57

<211> 743

<212> DNA

<213> Echovirus E18 (isolate E18-398/HeB/CHN/2015)

<400> 57

ttataacagc ctgtgggttg tacccaccca cagggcccac cgggcgctag cacactggta 60

tctcggtacc tttgtgcgcc tgttttatcc acccttctcc catttaattt agaagcacgc 120

gccaagacgg tcaataggga gcccagtaag ccaattgggc catgaccaag cacttctgta 180

accccgggcc gagtatcaat aagctgctcg cgcggctgaa ggagaaaaag ctcgttaccc 240

ggccaattac ttcgagaagc ccagtagcat catgaaagtt gcgcagtgtt tcgctccgca 300

caaccccagt gtagatcagg ccgatgagtc accgcgttcc ccacgggtga ccgtggcggt 360

ggctgcgctg gcggcctgcc catggggcca cccatgggac gcttcaatac tgacatggtg 420

tgaagagtct attgagctaa ttggtagtcc tccggcccct gaatgcggct aatcctaact 480

gcggagcaga tgcccacgca ccagtgggaa gtctgtcgta atgggtaact ctgcagcgga 540

accgactact ttgggtgtcc gtgtttcctt ttatttctac tctggctgct tatggtgaca 600

attgaaaggt tgttaccata tagctattgg attggccatc cggtgacaaa tagagcgatt 660

atttacttgt ttgttgggtt tgtaccactg aactacaaag ctttaaacac tcttaactac 720

attttaatcc ttaactcgtt aaa 743

<210> 58

<211> 743

<212> DNA

<213> Echovirus E18 (isolate E18-HeB15-54462/HeB/CHN/2015)

<400> 58

ttataacagc ctgtgggttg tacccaccca cagggcccac agggggccag cacactggta 60

tctcggtacc tttgtgggcc tgttttatcc acccttcccc cattttattt agaagcatgc 120

accaaaacgg tcaacaggga gcccagtaag ccacttgttc attgaccaag tacttctgtt 180

accccggacc gagtatcaat aggctgctcg cgcggctgaa ggagaaaacg ttcgttaccc 240

ggccaattac ttcgagaaac ccagtagcgc catggaggtt gcgcagcgtt tcgctccgca 300

caaccccagt gtagatcagg tcgatgagcc accgcgttcc ccacgggcga ccgtggcggt 360

ggctgcgttg gcggcctgcc catgggacta cccatgggac gcttcaatac tgacatggtg 420

tgaaaagtct attgagctaa ttggtagtcc tccggcccct gaatgcggct aatcctaact 480

gcggagcaga cgcccacaca ccagtgggcg atctgtcgta atgggcaact ctgcagcgga 540

accgactact ttgggtgtcc gtgtttcctt ttattcttac cctggctgct tatggtgaca 600

attgagaagt tgttgccata tagctattgg attggccatc cggtgacgag cagggcgata 660

atttatttgt ttatcgggtt tgtaccactg aactacaagg ttttaaacac tttggatttt 720

attctgatcc tcaactcact aaa 743

<210> 59

<211> 743

<212> DNA

<213> Echovirus E18 (isolate E18-HeB15-54498/HeB/CHN/2015)

<400> 59

ttaaaacagc ttgtgggttg ttcccaccca cagggcccac tgggcgctag cacactggta 60

tctcggtacc tttgtgcgcc tgttttatcc acccttttcc catttaactt agaagcacgc 120

gccaagacgg tcaataggga gcccagtaag ccaattgggc tatgaccaag cacttctgta 180

accccgggcc gagtatcaat aagctgctcg cgcggctgaa ggagaaaaag ctcgttaccc 240

ggccaattac ttcgagaaac ctagtagcat catgaaagtt gcgcagtgtt tcgctccaca 300

caaccccagt gtagatcagg ccgatgagtt accgcgttcc ccacgggtga ccgtggcggt 360

ggctgcgctg gcggcctgcc catggggcca cccatgggac gcttcaatac tgacatggtg 420

tgaagagtct attgagctaa ttggtagtcc tccggcccct gaatgcggct aatcctaact 480

gcggagcaga tgcccacgta ccagtgggaa gtctgtcgta acgggtaact ctgcagcgga 540

accgactact ttgggtgtcc gtgtttcctt ttatttctac tctggctgct tatggtgaca 600

attgaaaggt tgttaccata tagctattgg attggccatc cggtgtcaaa tagagcgatt 660

atttacttgt ttgttgggtt tgtaccactg gactacaaag ctttaaacac tcttgactac 720

attttaatcc ttaactcatt aaa 743

<210> 60

<211> 647

<212> DNA

<213> Echovirus E18 (isolate ETH_P12/E18_2016)

<400> 60

atacagtaac atagaagtca gaaacctaca cgaccaatag caggcgtgac gcaccagtca 60

tgtcttggtc aaacacttct gtttccccgg actgagtatc aataagctgc tcacgcggct 120

gaaggagaaa acgttcgtta tccggctaac tacttcgaga aacctagtaa caccatgaaa 180

gttgcagagt gtttcgctca gcacttcccc ccgtgtagat caggtcgatg agtcactgca 240

tcccccacgg gcgaccgtgg cagtggctgc gttggcggcc tgcctatggg gcaacccata 300

ggacgctcta atacagacat ggtgtgaaga gtctattgag ctarytrgta gtcctccggc 360

ccctgaatgc ggctaatccc aactgcggag caggcaccca caaaccagcg ggaagtctgt 420

cgtaatgggc aactctgcag cggaaccgac tactttgggt gtccgtgttt ccttttattc 480

atattttggc tgcttatggt gacaattgaa aggttgttac catatagcta ctggattggc 540

catccggtga caaatagagc aattatctat ttgtttgttg gttttatacc gctgaaccac 600

aaggttttaa ataaccttaa atacattata ttactgaaca ccttaaa 647

<210> 61

<211> 290

<212> DNA

<213> Echovirus E18 (isolate NSW-V13A-2008-ECHO18)

<400> 61

ggcccctgaa tgcggctaat cctaactgcg gagcagacgc tcacacacca gtgggaagtc 60

tgtcgtaatg ggcaactctg cagcggaacc gactactttg ggtgtccgtg tttcctttta 120

ttcttaccct ggctgcttat ggtgacaatt gaaaggttgt taccatatag ctattggatt 180

ggccacccgg tgacaagtag agcgattatt tatttgtttg ttgggtttgt accactgaac 240

tacaaggttt taaacacttt agacttcatt ctaatcctta actcactaaa 290

<210> 62

<211> 744

<212> DNA

<213> Echovirus E18 (strain A83/YN/CHN/2016)

<400> 62

ttaaaacagc cccgtgggtt gttcccaccc acagggccca ccgggcgcta gcacactggt 60

atctcggtac ctttgtgcgc ctgttttatc cacccttttc ccatttaatt tagaagcacg 120

cgccaagacg gtcaataggg agcccagtaa gccaattggg ctatgaccaa gcacttctgt 180

aaccccgggc tgagtatcaa taagctgctc gcgtggctga aggagaaaaa gctcgttacc 240

cggccaatta cttcgagaaa cccagtagca tcatgaaagt tgcgcagtgt ttcgctccac 300

acaaccccag tgtagatcag gccgatgagt caccgcgttc cccacgggtg accgtggcgg 360

tggctgcgct ggcggcctgc ccatggggcc acccatggga cgcttcaata ctgacatggt 420

gtgaagagtc tattgagcta attggtagtc ctccggcccc tgaatgcggc taatcctaac 480

tgcggagcag atgcccacgc accagtgggg agtctgtcgt aacgggtaac tctgcagcgg 540

aaccgactac tttgggtgtc cgtgtttcct tttatttcta ctctggctgc ttatggtgac 600

aattgaaagg ttgttaccat atagctattg gattggccat ccggtgacaa atagagcgat 660

tatttacttg tttgttgggt ttgtgccact gaactacaaa gctttaaaca ctcttaacta 720

cattttaatc cttaactcat taaa 744

<210> 63

<211> 743

<212> DNA

<213> Echovirus E18 (strain A86/YN/CHN/2016)

<400> 63

ttataacagc ctgtgggttg tacccaccca gagggcccac cgggcgctag cagggtggta 60

tctcggtacc tttgtgcgcc tgttttatcc gcccttctcc catttaattt agaagcacgc 120

gccaagacgg tcaataggga gcccagtaag ccaattgggc catgaccaag cacttctgta 180

accccgggcc gagtatcaat aagctgctcg cgcggctgaa ggagaaaaag ctcgttaccc 240

ggccaattac ttcgagaagc ccagtagcat catgaaagtt gcgcagtgtt tcgctccgca 300

caaccccagt gtagatcagg ccgatgagtc accgcgttcc ccacgggtga ccgtggcggt 360

ggctgcgctg gcggcctgcc catggggcca cccatgggac gcttcaatac tgacatggtg 420

tgaagagtct attgagctaa ttggtagtcc tccggcccct gaatgcggct aatcctaact 480

gcggagcaga tgcccacgca ccagtgggaa gtctgtcgta atgggtaact ctgcagcgga 540

accgactact ttgggtgtcc gtgtttcctt ttatttctac tctggctgct tatggtgaca 600

attgaaaggt tgttaccata tagctattgg attggccatc cggtgacaaa tagagcgatt 660

atttacttgt ttgttgggtt tgtgccactg aactacaaag ctttaaacac tcttaactac 720

attttaatcc ttaactcatt aaa 743

<210> 64

<211> 738

<212> DNA

<213> Echovirus E18 (isolate Jena/ST9524/10)

<400> 64

cagcctgtgg gttgtaccca cccacagggc ccacgtggcg ctagcacact ggtattccgg 60

tacctttgtg cgcctgtttt atccaccccg tcccccactg taacttagaa gatatgcaac 120

cctgatcaac agcaggtgtg acactccagt cacatcacga tcaagcactt ctgttgcccc 180

ggaccgagta tcaataagcc actcgcgtgg ctgaaggaga aaacgttcgt tatccgacca 240

actacttcga gaaacctagt accaccctgg aagttgcgta gcgtttcgct ccacacaacc 300

ccagtgtaga tcgggtcgat gagtcaccgc gttcctcacg ggcgaccgtg gcggtggctg 360

cgttggcggc ctgcccatgg ggcaacccat gggacgcttc aatactgaca tggtgcgaag 420

agtctattga gctagttggt ggtcctccgg cccctgaatg cggctaatcc taactgcgga 480

gcagataccc acatgccagt gggcgatctg tcgtaatggg taactctgca gcggaaccga 540

ctactttggg tgtccgtgtt tccttttatt cttatattgg ctgcttatgg tgacaattga 600

gaagttgtta ccatatagct attggattgg ccatccggtg acaaatagag cgattgttta 660

tttgtttgtt ggatttatac cactgaactt caaggtttta aacaccctca attttgtact 720

agttcttaat acatcaaa 738

<210> 65

<211> 734

<212> DNA

<213> Echovirus E18 (isolate Jena/VI10227/10)

<400> 65

ctgtgggttg tacccaccca cagggcccac gtggcgctag cacactggta ttccggtacc 60

tttgtgcgcc tgttttatcc accccatccc ccactgtaac ttagaagata tgcaaccctg 120

atcaacagca ggtgtgacac tccagtcaca tcacgatcaa gcacttctgt tgccccggac 180

cgagtatcaa taagccactc gcgtggctga aggagaaaac gttcgttatc cgaccaacta 240

cttcgagaaa cctagtacca ccctggaagt tgcgtagcgt ttcgctccac acaaccccag 300

tgtagatcgg gtcgatgagt caccgcgttc ctcacgggcg accgtggcgg tggctgcgtt 360

ggcggcctgc ccatggggca acccatggga cgcttcaata ctgacatggt gcgaagagtc 420

tattgagcta gttggtagtc ctccggcccc tgaatgcggc taatcctaac tgcggagcag 480

atacccacat gccagtgggc gatctgtcgt aatgggtaac tctgcagcgg aaccgactac 540

tttgggtgtc cgtgtttcct tttattctta tattggctgc ttatggtgac aattgagaag 600

ttgttaccat atagctattg gattggccat ccggtgacaa atagagcgat tgtttatttg 660

tttgttggat ttgtaccact gaacttcaag gttttaaaca ccctcaattt tgtactagtt 720

cttaacacat taaa 734

<210> 66

<211> 743

<212> DNA

<213> Echovirus E18 (isolate Kor05-ECV18-054cn)

<400> 66

ttaaaacagc ctgtgggttg tccccatcca cagggcccac cgggcgctag cacactggta 60

tctcggtacc tttgtgcgcc tgttttatcc acccttttcc catccaactt agaagcacgc 120

accaaaacgg tcaataggaa gcctagtaag ccaactgggt tttgaccaag cacttctgtt 180

tccccggacc gagtatcaat aggctgctcg cgcggctgaa ggagaaaacg ttcgttaccc 240

ggccaattac ttcgagaaac ccagtagcac catgaaggtt gcgcagtgtt tcgctccgca 300

catccccagt gcagatcagg ccgatgagcc accgcgtccc ccacgggcga ccgtggcggt 360

ggctgcgttg gcggcctgcc catgggacca cccatgggac gcttcaatac tgacatggtg 420

tgaagagtct attgagctaa ttggtagtcc tccggcccct gaatgcggct aatcctaact 480

gcggagcaga tgcccacaca ccagtgggaa gtctgtcgta atgggtaact ctgcagcgga 540

accgactact ttgggtgtcc gtgtttcctt ttattcttac tctggctgct tatggtgaca 600

attgaaaggt tgttaccata tagctattgg attggccacc cggtgacaag taaagcgatt 660

atttatttgt ttgttgggtt tgtaccactg aactataagg ctttaaacac tcttaacttc 720

attctaatcc ttaattcact aaa 743

<210> 67

<211> 725

<212> DNA

<213> Echovirus E19 (strain HAI/2016-23039B)

<400> 67

ttgatcccac ccacagggcc catagggcgt tagcactctg gtattgcggt acctttgtgc 60

gcctgtttta tattcccacc cccaatcgca acttagaagc agtacttcac gaccaacagt 120

gggcgtgaca caccagccac gtctcggtca agcacttctg tctccccgga ctgagtatca 180

ataagctgct cacgcggctg aaggagaaaa cgtccgttat ccggccaatt acttcgagaa 240

acctagtacc gccatgaaag ttgcgcagcg tttcgatccg cacaacccca gtgtagatca 300

ggtcgatgag tcaccgcgtt ccccacgggt gaccgtggcg gtggctgcgt tggcggcctg 360

cccatgggag aacccatggg acgcttcaat actgacatgg tgtgaagagt ctattgagct 420

aattggtagc cctccggccc ctgaatgcgg ctaatcctaa ctgcggagca gattcccgca 480

tgccagcggg tggtctgtcg taacgggcaa ctctgcagcg gaaccgacta ctttgggtgt 540

ccgtgtttcc ttttattcct gtatcggctg cttatggtga caattgagag attgttacca 600

tatagctatt ggattggcca tccggtgaca aacagagcaa ttatttacca atttattggt 660

tttgtaccat tgaattttaa cgtattcaaa actcttaacc ttattttggc tcttaataca 720

gcaaa 725

<210> 68

<211> 523

<212> DNA

<213> Echovirus E19 (strain HAI/2016-23036D)

<400> 68

ggaaaaaacg tccgttatcc ggccaattac ttcaagaaac ccagtaccac catgaaggtt 60

gcgcagcgtt tcgctccgca caaccccagt gtagatcagg tcgatgagtc accgcattcc 120

tcacgggcga ccgtggcggt ggctgcgctg gcggcctgcc catggggcaa cccatgggac 180

gcttcaatac tgacatggtg cgaagagtca attgagctaa ttggtagtcc tccggcccct 240

gaatgcggct aatcctaact gtggagcaga ttcccgcatg ccagcgggtg gtctgtcgta 300

acgggcaact ctgcagcgga accgactact ttgggtgtcc gtgtttcctt ttattcctat 360

atcggctgct tatggtgaca attgagagat tgttaccata tagctattgg attggccatc 420

cggtgacaaa cagagcaatt atttaccaat ttattggttt tgtaccattg aattttaacg 480

tattcaaaac tcttaacctt attttggctc ttaatacagc aaa 523

<210> 69

<211> 716

<212> DNA

<213> Echovirus E19 (strain HAI/2016-23037D)

<400> 69

cccacagggc ccatagggcg ctagcactct ggtattgcgg tacctttgtg cgcctgtttt 60

atattcccac ccccaatcgc aacttagaag cagtacttca cgaccaacag tgggcgtgac 120

acaccagcca cgtctcggtc aagcacctct gtctccccgg actgagtatc aataagctgc 180

tcacgcggct gagggagaaa acgtccgtta cccggccaat tacttcgaga aacctagtac 240

caccacgaaa gttgcgcagc gtttcgatcc gcacaacccc agtgtagatc aggtcgatga 300

gtcaccgcgt tccccacggg cgaccgtggc ggtggctgcg ttggcggcct gcccatggga 360

gaacccatgg gacgcttcaa tactgacatg gtgtgaagag tctattgagc taattggtag 420

tcctccggcc cctgaatgcg gctaatccta actgtggagc agattcccgc atgccagcgg 480

gtggtctgtc gtaacgggca actctgcagc ggaaccgact actttgggtg accgtgtttc 540

cttttattcc tatattggct gcttatggtg acagttgaga gattgttacc atatagctat 600

tggattggcc atccggtgac aaacagagca attatctacc aatttatcgg ttttgtacca 660

ttgaatttca acgcattcaa aaccctcaac tttattttga ctcttaatac agcaaa 716

<210> 70

<211> 579

<212> DNA

<213> Echovirus E19 (strain HAI/2016-23037E)

<400> 70

accaagcact tctgtytccc cggactgagt atcaataagc tgctcacgcg gctgaaggag 60

aaaacgtccg ttatccggcc aattacttcg agaaacctag taccgccatg aaagttgcgc 120

agcgtttcga tccgcacaac cccagtgtag atcaggtcga tgagtcaccg cgttccccac 180

gggtgaccgt ggcggtggct gcgttggcgg cctgcccatg ggagaaccca tgggacgctt 240

caatactgac atggtgtgaa gagtctattg agctaattgg tagccctccg gcccctgaat 300

gcggctaatc ctaactgcgg agcagattcc cgcatgccag cgggtggtct gtcgtaacgg 360

gcaactctgc agcggaaccg actactttgg gtgtccgtgt ttccttttat tcctatatcg 420

gctgcttatg gtgacaattg agagattgtt accatatagc tattggattg gccatccggt 480

gacaaacaga gcaattattt accaatttat tggttttgta ccattgaatt ttaacgtatt 540

caaaactctt aaccttattt tggctcttaa tacagcaaa 579

<210> 71

<211> 681

<212> DNA

<213> Echovirus E19 (strain HAI/2016-23042B)

<400> 71

gcggtacctt tgtgcgcctg ttttatattc ccacccccca atcgcaactt agaagcagta 60

cttcacgacc aacagtgggc gtgacacacc agccacgtct cggtcaagca cctctgtctc 120

cccggactga gtatcaataa gctgctcacg cggctgaggg agaaaacgtc cgttacccgg 180

ccaattactt cgagaaacct agtaccacca cgaaagttgc gcagcgtttc gatccgcaca 240

accccagtgt agatcaggtc gatgagtcac cgcgttcccc acgggcgacc gtggcggtgg 300

ctgcgttggc ggcctgccca tgggaaaacc catgggacgc ttcaatactg acatggtgtg 360

aagagtctat tgagctaatt ggtagtcctc cggcccctga atgcggctaa tcctaactgc 420

ggagcagatt cccgcatgcc agcgggtggt ctgtcgtaac gggcaactct gcagcggaac 480

cgactacttt gggtgtccgt gtttcctttt attcctatat tggctgctta tggtgacaat 540

tgagagattg ttaccatata gctattggat tggccatccg gtgacaagca gagcaattat 600

ctaccaattt attggttttg taccattgaa tttcaacgca ttcaaaactc ttaactttat 660

tttgactctt aatacagcaa a 681

<210> 72

<211> 727

<212> DNA

<213> Echovirus E19 (strain HAI/2016-23046B)

<400> 72

ggttgatccc acccacaggg cccatagggc gctagcactc tggtattgcg gtacctttgt 60

gcgcctgttt tatattccca cccccaattg caacttagaa gcagtactta acgaccaaca 120

gtgggcgtga cacaccagcc acgtctcggt caagcacttc tgtctccccg gactgagtat 180

caaaaagctg ctcacgcggc tgaaggagaa aacgtccgtt atccggccaa ttacttcgag 240

aaacctagta ccgccatgaa agttgcgcag cgtttcgatc cgcacaaccc cagtgtagat 300

caggtcgatg agtcaccgcg ttccccacgg gtgaccgtgg cggtgactgc gttggcggcc 360

tgcccatggg agaacccatg ggacgcttca atactgacat ggtgtgaaga gtctattgag 420

ctaattggta gtcctccggc ccctgaatgc ggctaatcct aactgcggag cagattcccg 480

catgccagcg ggtggtctgt cgtaacgggc aactctgcag cggaaccgac tactttgggt 540

gtccgtgttt ccttttattc ttatattggc tgcttatggt gacaattgag agattgttac 600

catatagcta ttggattggc catccggtaa caaacagagc aattatttac caatttattg 660

gttttgtacc attgaatttt aacacattca aaactcttaa ccttatcttg gctcttaata 720

cagcaaa 727

<210> 73

<211> 725

<212> DNA

<213> Echovirus E19 (strain HAI/2016-23047)

<400> 73

ttgatcccac ccacagggcc catagggcgc tagcactctg gtattgcggt acctttgtgc 60

gcctgtttta tattcccacc cccaattgca acttagaagc agtacttaac gaccaacagt 120

gggcgtgaca caccagccac gtctcggtca agcacttctg tctccccgga ctgagtatca 180

aaaagctgct cacgcggctg aaggagaaaa cgtccgttat ccggccaatt acttcgagaa 240

acctagtacc gccatgaaag ttgcgcagcg tttcgatccg cacaacccca gtgtagatca 300

ggtcgatgag tcaccgcgtt ccccacgggt gaccgtggcg gtgactgcgt tggcggcctg 360

cccatgggag aacccatggg acgcttcaat actgacatgg tgtgaagagt ctattgagct 420

aattggtagt cctccggccc ctgaatgcgg ctaatcctaa ctgcggagca gattcccgca 480

tgccagcggg tggtctgtcg taacgggcaa ctctgcagcg gaaccgacta ctttgggtgt 540

ccgtgtttcc ttttattctt atattggctg cttatggtga caattgagag attgttacca 600

tatagctatt ggattggcca tccggtaaca aacagagcaa ttatttacca atttattggt 660

ttcgtaccat tgaattttaa cacattcaaa actcttaacc ttatcttggc tcttaataca 720

gcaaa 725

<210> 74

<211> 725

<212> DNA

<213> Echovirus E19 (strain HAI/2016-23054)

<400> 74

ttgatcccac ccacagggcc catagggcgt tagcactctg gtattgcggt acctttgtgc 60

gcctgtttta tattcccacc cccaatcgca acttagaagc agtacttcac gaccaacagt 120

gggcgtgaca caccagccac gtctcggtca agcacttctg tctccccgga ctgagtatca 180

ataagctgct cacgcggctg aaggagaaaa cgtccgttat ccggccaatt acttcgagaa 240

acctagtacc gccatgaaag ttgcgcagcg tttcgatccg cacaacccca gtgtagatca 300

ggtcgatgag tcaccgcgtt ccccacgggt gaccgtggcg gtggctgcgt tggcggcctg 360

cccatgggag aacccatggg acgcttcaat actgacatgg tgtgaagagt ctattgagct 420

aattggtagc cctccggccc ctgaatgcgg ctaatcctaa ctgcggagca gattcccgca 480

tgccagcggg tggtctgtcg taacgggcaa ctctgcagcg gaaccgacta ctttgggtgt 540

ccgtgtttcc ttttattcct gtatcggctg cttatggtga caattgagag attgttacca 600

tatagctatt ggattggcca tccggtgaca aacagagcaa ttatttacca atttattggt 660

tttgtaccat tgaattttaa cgtattcaaa actcttaacc ttattttggc tcttaataca 720

gcaaa 725

<210> 75

<211> 725

<212> DNA

<213> Echovirus E19 (strain HAI/2016-23052)

<400> 75

ttgatcccac ccacagggcc catagggcgt tagcactctg gtatcacggt acctttgtgc 60

gcctgtttta tattcccacc cccaatcgca acttagaagc agtacttcac gaccaacagt 120

gggcgtgaca caccagccac gtctcggtca agcacttctg tctccccgga ctgagtatca 180

ataagctgct cacgcggctg aaggaaaaaa cgtccgttat ccggccaatt acttcgagaa 240

acctagtacc gccatgaaag ttgcgcagcg tttcgatccg cacaacccca gtgtagatca 300

ggccgatgag tcaccgcgtt ccccacgggt gaccgtggcg gtggctgcgt tggcggcctg 360

cccatggggg aacccatggg acgcttcaat actgacatgg tgtgaagagt ctattgagct 420

aattggtagc cctccggccc ctgaatgcgg ctaatcctaa ctgcggagca gattcccgca 480

tgccagcggg tggtctgtcg taacgggcaa ctctgcagcg gaaccgacta ctttgggtgt 540

ccgtgtttcc ttttattcct atatcggctg cttatggtga caattgagag attgttacca 600

tatagctatt ggattggcca tccggtgaca aacagagcaa ttatttacca atttattggt 660

tttgtaccat tgaattttaa cgtattcaaa actcttaacc ttattttggc tcttaataca 720

gcaaa 725

<210> 76

<211> 724

<212> DNA

<213> Echovirus E19 (strain HAI/2016-23053)

<400> 76

tgatcccacc cacagggccc atagggcgtt agcactctgg tatcacggta cctttgtgcg 60

cctgttttat attcccaccc ccaatcgcaa cttagaagca gtacttcacg accaacagtg 120

ggcgtgacac accagccacg tctcggtcaa gcacttctgt ctccccggac tgagtatcaa 180

taagctgctc acgcggctga aggaaaaaac gtccgttatc cggccaatta cttcgagaaa 240

cctagtaccg ccatgaaagt tgcgcagcgt ttcgatccgc acaaccccag tgtagatcag 300

gccgatgagt caccgcgttc cccacgggtg accgtggcgg tggctgcgtt ggcggcctgc 360

ccatggggga acccatggga cgcttcaata ctgacatggt gtgaagagtc tattgagcta 420

attggtagcc ctccggcccc tgaatgcggc taatcctaac tgcggagcag attcccgcat 480

gccagcgggt ggtctgtcgt aacgggcaac tctgcagcgg aaccgactac tttgggtgtc 540

cgtgtttcct tttattccta tatcggctgc ttatggtgac aattgagaga ttgttaccat 600

atagctattg gattggccat ccggtgacaa acagagcaat tatttaccaa tttattggtt 660

ttgtaccatt gaattttaac gtattcaaaa ctcttaacct tattttggct cttaatacag 720

caaa 724

<210> 77

<211> 577

<212> DNA

<213> Echovirus E19 (train HAI/2016-23062D)

<400> 77

caagcacttc tgtatccccg gactgagtat cagtaggctg ctcgcgcggc tgaaggagaa 60

aacgttcgtt acccggccac ttacttcgag aaacctagta acgccatgaa agttgcgcag 120

cgtttcgatc cgcacaaccc cagtgtagat caggtcgatg agtcaccgcg ttccccacgg 180

gcgaccgtgg cggtggctgc gttggcggcc tgcccatggg agaacccatg ggacgcttca 240

atactgacat ggtgtgaaga gtctattgag ctaattggta gtcctccggc ccctgaatgc 300

ggctaatcct aactgtggag cagattcccg catgccagcg ggtggtctgt cgtaacgggc 360

aactctgcag cggaaccgac tactttgggt gtccgtgttt ccttttattc ctatattggc 420

tgcttatggt gacaattgag agattgttac catatagcta ttggattggc catccggtga 480

caaacagagc gattatctac caatttattg gttttgtacc attgaatttc aacgcattca 540

aaactcttaa ctttattttg actcttaata cagcaaa 577

<210> 78

<211> 701

<212> DNA

<213> Echovirus E19 (train HAI/2016-23063B)

<400> 78

gggcgctagc actctggtat tgcggtacct ttgtgcgcct gttttatgtt cccaccccca 60

atcgcaactt agaagcggta cttcacgacc aacagtgggc gtgacacacc agccacgtct 120

cggtcaagca cttctgtctc cccggactga gtatcaatag gctgctcacg cggctgaagg 180

agaaaacgtc cgttatccgg ccaattactt cgagaaacct agtaccgcca tgaaagttgc 240

gcagcgtttc gatccgcaca accccagtgt agatcaggtc gatgagtcac cgcgttcccc 300

acgggtgacc gtggcggtgg ctgcgttggc ggcctgccca tgggagaacc catgggacgc 360

ttcaatactg acatggtgtg aagagtctat tgagctaatt ggtagtcctc cggcccctga 420

atgcggctaa tcctaactgc ggagcagatt cccgcatgcc agcgggtggt ctgtcgtaac 480

gggcaactct gcagcggaac cgactacttt gggtgtccgt gtttcctttt attcctatat 540

tggctgctta tggtgacaat tgagagattg ttaccatata gctattggat tggccatccg 600

gtgacaaaca gagcgattat ttaccaattt attggttttg taccgttgaa ttttaacaca 660

ttcagaactc ttaaccttat tttggctctt aatacagcaa a 701

<210> 79

<211> 715

<212> DNA

<213> Echovirus E19 (train HAI/2016-23064B)

<400> 79

ccacagggcc catagggcgc tagcactctg gtattgcggt acctttgtgc gcctgtttta 60

tgttcccacc cccaatcgca acttagaagc ggtacttcac gaccaacagt gggcgtgaca 120

caccagccac gtctcggtca agcacttctg tctccccgga ctgagtatca ataggctgct 180

cacgcggctg aaggagaaaa cgtccgttat ccggccaatt acttcgagaa acctagtacc 240

gccatgaaag ttgcgcagcg tttcgatccg cacaacccca gtgtagatca ggtcgatgag 300

tcaccgcgtt ccccacgggt gaccgtggcg gtggctgcgt tggcggcctg cccatggggt 360

aacccatggg acgcttcaat actgacatgg tgtgaagagt ctattgagct aattggtagt 420

cctccggccc ctgaatgcgg ctaatcctaa ctgcggagca gattcccgca tgccagcggg 480

tggtctgtcg taacgggcaa ctctgcagcg gaaccgacta ctttgggtgt ccgtgtttcc 540

ttttattcct atattggctg cttatggtga caattgagag attgttacca tatagctatt 600

ggattggcca tccggtgaca aacagagcga ttatttacca atttattggt tttgtaccgt 660

tgaattttaa cacattcaga actcttaacc ttattttggc tcttaataca gcaaa 715

<210> 80

<211> 558

<212> DNA

<213> Echovirus E19 (strain HAI/2016-23067B)

<400> 80

ggactgagta tcaataagct gctcacgcgg ctgagggaga aaacgtccgt tacccggcca 60

attacttcga gaagcctagt accaccacga aagttgcgca gcgtttcgat ccgcacaacc 120

ccagtgtaga tcaggtcgat gagtcaccgc gttccccacg ggcgaccgtg gcggtggctg 180

cgttggcggc ctgcccatgg gagaacccat gggacgcttc aatactgaca tggtgtgaag 240

agtctattga gctaattggt agtcctccgg cccctgaatg cggctaatcc taactgtgga 300

gcagattccc gcatgccagc gggtggtctg tcgtaacggg caactctgca gcggaaccga 360

ctactttggg tgtccgtgtt tccttttatt cctatattgg ctgcttatgg tgacaattga 420

gagattgtta ccatatagct attggattgg ccatccggtg acaaacagag caattatcta 480

ccaatttatt ggttttgtac cattgaatta caacgcattc aaaactctta actttatttt 540

ggctcttaat acagcaaa 558

<210> 81

<211> 726

<212> DNA

<213> Echovirus E19 (strain HAI/2016-23070B)

<400> 81

tttgatccca cccacagggc ccatagggcg ctagcactct ggtattgcgg tacctttgtg 60

cgcctgtttt atattcccac ccccaatcgc aacttagaag cagtacttca cgaccaacag 120

tgggcgtgac aaaccagcca cgtctcggtc aagcacttct gtctccccgg actgagtatc 180

aataagctgc tcacgcggct gagggagaaa acgtccgtta tccggccgat tacttcgaga 240

aacccagtac caccacgaaa gttgcgcagc gtttcgatcc gcacaacccc agtgtagatc 300

aggtcgatga gtcaccgcgt tccccacggg tgaccgtggc ggtggctgcg ttggcggcct 360

gcccatgggg gaacccatgg gacgcttcaa tactgacatg gtgtgaagag tctattgagc 420

taattggtag tcctccggcc cctgaatgcg gctaatccta actgtggagc agattcccgc 480

atgccagcgg gtggtctgtc gtaacgggca actctgcagc ggaaccgact actttgggtg 540

tccgtgtttc cctttaatcc tatattggct gcttatggtg acaattgaga gattgttacc 600

atatagctat tggattggcc atccggtgac aaacagagca attatttacc aatttattgg 660

ttttgtgcca ttgaatttca acgcattcaa aactcttaac cttattttga ctcttaatac 720

agcaaa 726

<210> 82

<211> 725

<212> DNA

<213> Echovirus E19 (strain HAI/2017-23079)

<400> 82

ttgatcccac ccacagggcc catagggcgc tagcactctg gtattgcggt acctttgtgc 60

gcctgtttta tattcccacc cccaatcgca acttagaagc agtacttcac gaccaacagt 120

gggcgtgaca aaccagccac gtctcggtca agcacttctg tctccccgga ctgagtatca 180

ataagctgct cacgcggctg agggagaaaa cgtccgttat ccggccaatt acttcgagaa 240

acccagtacc accacgaaag ttgcgcagcg tttcgatccg cacaacccca gtgtagatca 300

ggtcgatgag tcaccgcgtt ccccacgggt gaccgtggcg gtggctgcgt tggcggcctg 360

cccatggggg aacccatggg acgcttcaat actgacatgg tgtgaagagt ctattgagct 420

aattggtagt cctccggccc ctgaatgcgg ctaatcctaa ctgtggagca gattcccgca 480

tgccagcggg tggtctgtcg taacgggcaa ctctgcagcg gaaccgacta ctttgggtgt 540

ccgtgtttcc ctttaatcct atattggctg cttatggtga caattgagag attgttacca 600

tatagctatt ggattggcca tccggtgaca aacagagcaa ttatttacca atttattggt 660

tttgtaccat tgaatttcaa cgcattcaaa actcttaacc ttattttgac tcttaataca 720

gcaaa 725

<210> 83

<211> 680

<212> DNA

<213> Echovirus E19 (strain HAI/2017-23081A)

<400> 83

gcggtacctt tgtgcgcctg ttttatattc ccacccccaa acgcaactta gaagcagtac 60

ttcacgacca acagtgggcg tggcacacca gccacgtctc ggtcaagcac ttctgtctcc 120

ccggactgag tatcaataag ctgatcgcgc ggctgaagga gaaaacgtcc gttatccggc 180

caattacttc gagaaaccta gtaccaccat gaaagttgcg tggcgtttcg atccgcacaa 240

ccccagtgta gatcaggtcg atgagtcacc gcgttcccca cgggtgaccg tggcggtggc 300

tgcgttggcg gcctgcccat gggggaaccc atgggacgct tcaatactga catggtgtga 360

agagtctatt gagctaattg gtagtcctcc ggcccctgaa tgcggctaat cctaactgcg 420

gagcagattc ccgcatgcca gcgggtggtc tgtcgtaacg ggcaactctg cagcggaacc 480

gactactttg ggtgtccgtg tttcctttta ttcctatatt ggctgcttat ggtgacaatt 540

gagagattgt taccatatag ctattggatt ggccatccgg tgacaaacag agcaattatt 600

taccaattta ttggttttgt accattgaat tttaacgcat tcaaaactct taaccttatt 660

ttggctctta atacatcaaa 680

<210> 84

<211> 673

<212> DNA

<213> Echovirus E19 (isolate ETH_P3/E19_2016)

<400> 84

ctgtgtgcgc ctgttttatc caccctaccc tgtcgtaact tagaagttaa tcaactatgg 60

tcactaggcg gctcagtgtg ccaactgagt ctcgaccaag cacttctgtt accccggact 120

gagtatcaat aggctgctca cgcggctgaa ggagaaaacg ttcgttaccc ggccaattac 180

ttcgagaaac ctagtaccac catgaaggtt gcgcagcgtt tcgctccgca caaccccagt 240

gtagatcagg tcgatgagtc accgcattcc ccacgggcga ccgtggcggt ggctgcgttg 300

gcggcctgcc catggggcaa cccatgggac gcttcaatac tgacatggtg cgaagagtct 360

attgagctaa ttggtagtcc tccggcccct gaatgcggct aatcctaact gcggagcaga 420

cactcacaat ccagtgagca gtctgtcgta atgggcaact ctgcagcgga accgactact 480

ttgggtgtcc gtgtttcctt ttattcttat actggctgct tatggtgaca attgaaagat 540

tgttaccata tagctattgg attggccatc cagtgactaa tagagcgatc atttatttgt 600

ttgttggttt tgtcccatta aactataaag ttttgaacac acttaatttt atcatactgc 660

ttaactcatc aaa 673

<210> 85

<211> 742

<212> DNA

<213> Echovirus E19 (strain NGR_2014)

<400> 85

ttttaaagcc tgtgggttgt tcccacccac agggcccatt gggcgctagc actctggtat 60

tgcggtacct ttgtgcgcct gttttatatc cccctccccc atttgaaact tagaagcaag 120

acacaccgat caatagctga tatggcaagc cagccatatc ttgatcaagt acttctgttt 180

ccccggaccg agtatcaata gactgcttgc gcggtcgaag gagaaagcgt tcgttacccg 240

gctaattact tcgagaaacc tagtaacacc atgaaagttg cagagtgttt cgctcagcac 300

aaccccagtg tagatcaggc tgatgagtca ccgcattccc cacgggtgac cgtggcggtg 360

gctgcgttgg cggcctgcct gtggggcaac ccacaggacg ctctaataca gacatggtgt 420

gaagagtcta ttgagctaat tagtagtcct ccggcccctg aatgcggcta atcctaactg 480

cggagcacgt acccccattc caggaggtgg cgtgtcgtaa cgggtaactc tgcagcggaa 540

ccgactactt tgggtgtccg tgtttccttt tatctttaca ctggctgctt atggtgacaa 600

ttgaaagatt gttaccatat agctattgga ttggccatcc ggtgacaaat agagcgattg 660

tatatttgtt cattggattt gtaccactaa gctataaagc attaaacact cttaacctta 720

tcctagtgct taactcaaca aa 742

<210> 86

<211> 745

<212> DNA

<213> Echovirus E19 (isolate PDV_BLR_IN)

<400> 86

ttaaaacagg gtgtgggtta ttcccaccca cagggcccac tgggcgccag cacactggta 60

tcacggtacc cttgtgcgcc tgttttatat acccctcccc taaaatgtaa cttagaagca 120

aatccacaaa gaccaacagc agacacagcg cgccagttgt gttacggtca agcacttctg 180

ttaccccgga ccgagtatca ataagctact cacgtggctg aaggagaaaa cgttcgttac 240

ccgcccgatt acttcgagaa gcctagtaac gccatggagg ttgcgcggcg tttcgctccg 300

cacaatccca gtgtagatca ggtcgatgag ccaccgcatt ccccacgggt gaccgtggcg 360

gtggctgcgc tggcggcctg cccatggggc aacccatggg acgcttcaat actgacatgg 420

tgcgaagagc ctattgagct aattgggagt cctccggccc ctgaatgcgg ctaatcccaa 480

ctgcggagca ggcgcccacg tgccagtggg tagtctgtcg taacgggcaa ctctgcagcg 540

gaaccgacta ctttgggtgt ccgtgtttcc ttttattcct acattggctg cttatggtga 600

caattgagag attgttacca tatagctatt ggattggcca tccggcgaca aacagagcga 660

ttgtttattt gttcattggt tttataccac taaactatac aactttaaac accctcaatt 720

ttattttaac gcttaacaca gcaaa 745

<210> 87

<211> 742

<212> DNA

<213> Echovirus E19 (strain Burke)

<400> 87

ttaaaacagc ctgtgggttg tacccaccca cagggcccaa tgggcgctag cactccggta 60

ttacggtacc cttgtgcgcc tgttttattt ccccttcccc aactgaaact tagaagaaat 120

acacaatgat cgacagtaag cgtggcgcac cagccatgtt ttgatcaagc acttctgttt 180

ccccggacca agtatcaata gactgctcac gcggttgaag gagaaagcgt tcgttaaccg 240

gctaactact tcgagaaacc cagtaacacc atgaaagttg cagagtgttt cgctcagcac 300

tcccccagtg tggatcaggt cgatgagtca ctgcgttccc cacgggtgac cgtggcagtg 360

gctgcgttgg cggcctgcct gtggggcaac ccacaggacg ctctaatacg gacatggtgt 420

gaagagtcta ttgagctagt tagtagtcct ccggcccctg aatgcggcta atcctaactg 480

tggagcacat gcccctaacc caaggggtgg tgtgtcgtaa tgggtaactc cgcagcggaa 540

ccgactactt tgggtgaccg tgtttccttt tatttttaca ttggctgcat atggtgacga 600

ttgagagatt gttgccatat agctattgga ttggccatcc agtgtctaac agagctattg 660

tatatctttt tgttggattt gtaccacttg acttgaaaga ggtcaggaca ctacaataca 720

ttatactgct gaatactgca aa 742

<210> 88

<211> 722

<212> DNA

<213> Echovirus E19 (strain K/542/81)

<400> 88

tccccaccca cagggcccac tgggcgctag cacaccggta ttgcggtacc tttgtgcgcc 60

tgttttatct acccttcccc tacgtaactt agaagtacag cccatatggt caatagacag 120

ctcagtaaac caactgggct atgaccaagc acttctgtta ccccggactg agtatcaata 180

ggctgctcac gcggctgaag gagaaaacgt tcgttacccg gccaattact tcgaaaagct 240

tagtaccacc atggaggttg cgcagtgttt cgctccgcac aaccccagtg tagatcaggc 300

cgatgagtca ccgcgaccct cacgggcgac cgtggcggtg gctgcgctgg cggcctgccc 360

atggggcaac ccgtgggacg cttcaatact gacatggtgc gaagagccta ttgagctaat 420

tggtattcct ccggcccctg aatgcggcta atcccaactg cggagcagat acccacacac 480

cagtgggcag tctgtcgtaa cgggcgactc tgcagcggaa ccgactactt tgggtgtccg 540

tgtttccttt tattcattta ctggctgctt atggtgacaa ttgagaaatt gttaccatat 600

agctattgga ttggccatcc ggtgagcaac agagcgatta tatacctctt tgttggattc 660

atatcactta actttaacaa ttataacacc ctactttaca ttatttgttt gaacacgaaa 720

ag 722

<210> 89

<211> 687

<212> DNA

<213> Echovirus E20 (isolate E20/TO/BR/016)

<400> 89

ggtatcacgg tacctttgtg cgcctgtttt atgcaccccc cccccattgc aacttagaag 60

cacaccaaac acggtcaata ggcggttcag tgcgccaact gggcctcgac caagcacttc 120

tgttaccccg gaccgagtat caataggctg ctcgcgcggc tgaaggagaa aacgtccgtt 180

acccggccaa ttacttcggg aaacctagta ccaccatgga ggttgcgcgg cgtttcgctc 240

cacacagccc cagtgtagat caggtcgatg agtcaccgcg ttccctacgg gcgaccgtag 300

cggtggctgc gttggcggcc tgcccatggg gcgacccatg ggacgcttca atactgacat 360

ggtgtgaaga gtctactgag ctaattggta ttcctccggc ccctgaatgc ggctaatccc 420

aactgcggag cagataccca catgccagtg ggcagtctgt cgtaatgggt aactctgcag 480

cggaaccgac tactttgggt gtccgtgttt ccttttattc ttatattggc tgcttatggt 540

gacaattgaa agattgttgc catatagcta ttggattggc catccagtaa caaacagagc 600

gattgtttat ttgtttgttg gcttcgtgcc gttaaatcat aagattttaa aaacccttaa 660

tttcattttg ctacttaata gtacaaa 687

<210> 90

<211> 655

<212> DNA

<213> Echovirus E20 (strain HAI/2016-23038B)

<400> 90

acaccctccc ccgagtgcaa cttagaagat atccacacac ggtcaatagg cggctcagta 60

tgccaactga gtcatgacca agcacttctg ttcccccgga ctgagtatca ataagctgct 120

tacgtggctg aaggagaaaa cgttcgttat ccggccaatt acttcgagaa gcttagtacc 180

accatggagg ttgcgcagtg tttcgttccg cacaacccca gtgtagatca ggtcgatgag 240

tcaccgcact cctcacgggc gaccgtggcg gtggctgcgc tggcggcctg cccctggggc 300

aacccctggg acgctccaat actgacatgg tgcgaagagc ctattgagct aattggtagt 360

cctccggccc ctgaatgcgg ctaatcctaa ctgcggagca gacacccaca agccagtggg 420

cagtctgtcg tagtgggtaa ctctgcagcg gaaccgacta ctttgggtgt ccgtgtttcc 480

ttttattcct acattggctg cttatggtga caattgagag attgttacca tatagctatt 540

ggattggccg tccagtgaca aacagagcga ttatctatct atttgttggc ttcgtaccat 600

taaaccttag agtcctaaag accctcaatt tgattataca atttaactct gtgaa 655

<210> 91

<211> 736

<212> DNA

<213> Echovirus E20 (strain HAI/2016-23041B)

<400> 91

agcctgtggg ttgttcccac ccacagggcc caccgggcgc tagcacactg gtatcccggt 60

acctttgtgc gcctgtttta cacaccctcc cccgatggca acttagaaga tttccacaca 120

cggtcaatag gcggctcagt ataccaactg ggtcatgacc aagcacttct gttcccccgg 180

actgagtatc aataagctgc tcacgtggct gaaggagaaa acgttcgtta tccggccaat 240

tacttcgaga agcttagtac caccatagag gttgcgcagt gtttcgctcc gcacaacccc 300

agtgtagatc aggtcgatga gtcaccgcac tcctcacggg cgaccgtggc ggtggctgcg 360

ctggcggcct gcccatgggg caacccatgg gacgcttcaa tactgacatg gtgcgaagag 420

cctattgagc taattggtat tcctccggcc cctgaatgcg gctaatccta actgcggagc 480

agatacccac gagccagtgg gcagtctgtc gtaatgggta actctgcagc ggaaccgact 540

actttgggtg tccgtgtttc cttttattcc tatattggct gctaatggtg acaattgaga 600

gattgttgcc atatagctat tggattggcc atccggtgac aaacagagcg attatctatc 660

tatttgttgg cttcatacca ttaaacctta gagtcctaaa gaccctcaac ttaattatac 720

aatttaactc tgcgaa 736

<210> 92

<211> 685

<212> DNA

<213> Echovirus E20 (strain HAI/2016-23085B)

<400> 92

tatcacggta cctttgtgcg cctgttttat acaccctccc ccgagtgcaa cttagaagat 60

atccacacac ggtcaatagg cggctcagta tgccaactga gtcatgacca agcacttctg 120

ttcccccgga ctgagtatca ataagctgct tacgtggctg aaggagaaaa cgttcgttat 180

ccggccaatt acttcgagaa gcttagtacc accatggagg ttgcgcagtg tttcgttccg 240

cacaacccca gtgtagatca ggtcgatgag tcaccgcact cctcacgggc gaccgtggcg 300

gtggctgcgc tggcggcctg cccatggggc aacccatggg acgctccaat actgacatgg 360

tgcgaagagc ctattgagct aattggtagt cctccggccc ctgaatgcgg ctaatcctaa 420

ctgcggagca gacacccacg agccagtggg cagtctgtcg taatgggtaa ctctgcagcg 480

gaaccgacta ctttgggtgt ccgtgtttcc ttttattcct acattggctg cttatggtga 540

caattgagag attgttacca tatagctatt ggattggccg tccagtgaca aacagagcga 600

ttatctatct atttgttggc ttcgtaccat taaaccttag agtcctaaag accctcaatt 660

tgattataca atttaactct gtgaa 685

<210> 93

<211> 699

<212> DNA

<213> Echovirus E20 (strain HAI/2016-23065C)

<400> 93

cgctagcaca ctggtatccc ggtacctttg tgcgcctgtt ttatacaccc tcccccgaca 60

gcaacttaga agatttccac acacggtcaa taggcggctc agtatgccaa ctgggtcatg 120

accaagtact tctgtctccc cggactgagt atcaataagc tgctcacgtg gctgaaggag 180

aaaacgttcg ttatccggcc aattacttcg agaagcttag taccaccatg gaggttgcgc 240

agtgtttcgt tccgcacaac cccagtgtag atcaggtcga tgagtcaccg cactcctcac 300

gggcgaccgt ggcggtggct gcgctggcgg cctgcccatg gggcacccca tgggacgctt 360

caatactgac atggtgcgaa gagcctattg agctaattgg taatcctccg gcccctgaat 420

gcggctaatc ctaactgcgg agcagatacc ctcgagccag tgggcagtct gtcgtaatgg 480

gcaactctgc agcggaaccg actactttgg gtgtccgtgt ttccttttat tcctatattg 540

gctgcctatg gtgacaattg agagattgtt accatatagc tattggattg gccatccagt 600

gacaaacaga gcgattatct acttatttgt tggcttcata ccattaaatc ttagagtcct 660

aaagaccctc aacttgattg taaaatttaa ctctgtgaa 699

<210> 94

<211> 655

<212> DNA

<213> Echovirus E20 (strain HAI/2016-23068B)

<400> 94

acaccctccc ccgagtgcaa cttagaagat atccacacac ggtcaatagg cggctcagta 60

tgccaactga gtcatgatca agcacttctg ttcccccgga ctgagtatca ataagctgct 120

tacgtggctg aaggagaaaa cgttcgttat ccggccaatt acttcgagaa gcttagtacc 180

accatggagg ttgcgcagtg tttcgttccg cacaacccca gtgtagatca ggtcgatgag 240

tcaccgcact cctcacgggc gaccgtggcg gtggctgcgc tggcggcctg cccatggggc 300

aacccatggg acgcttcaat actgacatgg tgcgaagagc ctattgagct aattggtagt 360

cctccggccc ctgaatgcgg ctaatcctaa ctgcggagca gacacccaca agccagtggg 420

cagtctgtcg taatgggtaa ctctgcagcg gaaccgacta ctttgggtgt ccgtgtttcc 480

ttttattcct atattggctg cttatggtga caattgagag attgttacca tatagctatt 540

ggattggccg tccagtgaca aacagagcga ttatctatct atttgttggc ttcgtaccat 600

taaaccttag agtcctaaag accctcaatt tgattataca atttaactct gcgaa 655

<210> 95

<211> 725

<212> DNA

<213> Echovirus E20 (strain HAI/2016-23069)

<400> 95

tgttcccacc cacagggccc accgggcgct agcacactgg tatctcggta cctttgtgcg 60

cctgttttat acacccttcc ccgagtgcaa cttagaagat atccacacac ggtcaatagg 120

cggctcagta tgccaactga gtcatgacca agcacttctg ttcccccgga ctgagtatca 180

ataagctgct tacgtggctg aaggagaaaa cgttcgttat ccggccaatt acttcgagaa 240

gcttagtacc accatggagg ttgcgcagtg tttcgttccg cacaacccca gtgtagatca 300

ggtcgatgag tcaccgcact cctcacgggc gaccgtggcg gtggctgcgc tggcggcctg 360

cccatggggc aacccatggg acgcttcaat actgacatgg tgcgaagagc ctattgagct 420

aattggtagt cctccggccc ctgaatgcgg ctaatcctaa ctgcggagca gacacccacg 480

agccagtggg cggtctgtcg taatgggtaa ctctgcagcg gaaccgacta ctttgggtgt 540

ccgtgtttcc ttttatccat acattggctg cttatggtga caattgagag attgttacca 600

tatagctatt ggattggccg tccagtgacg aacagagcga ttatctatct atttgttggc 660

ttcgtaccat taaaccttag agtcctaaag accctcaatt tgattataca atttaactct 720

gcgaa 725

<210> 96

<211> 610

<212> DNA

<213> Echovirus E20 (strain HAI/2017-23080B)

<400> 96

ataggcggct cagtatgcca actgggtcat gaccaagcac ttctgtctcc ccggactgag 60

tatcaataag ctgctcacgt ggctgaagga gaaaacgttc gttatccggc caattacttc 120

gagaagctta gtaccaccat ggaggttgcg cagtgtttcg ttccgcacaa ccccagtgta 180

gatcaggtcg atgagtcacc gcactcctca cgggcgactg tggcggtggc tgcgctggcg 240

gcctgcccat ggggcacccc atgggacgct tcaatactga catggtgcga agagcctatt 300

gagctaattg gtaatcctcc ggcccctgaa tgcggctaat cctaactgcg gagcagatac 360

cctcgagcca gtgggcagtc tgtcgtaatg ggcaactctg cagcggaacc gactactttg 420

ggtgtccgtg tttcctttta ttcctatatt ggctgcttat ggtgacaatt gagagattgt 480

taccatatag ctattggatt ggccatccag tgacaaacag agcgattatc tacttatttg 540

ttggctttgt accattaaat cttagagtcc ttaagaccct caacttgatt atacaattta 600

actctgcgaa 610

<210> 97

<211> 699

<212> DNA

<213> Echovirus E20 (strain HAI/2017-23081B)

<400> 97

cgctagcaca ctggtatccc ggtacctttg tgcgcctgtt ttacgcaccc tcccccgaca 60

gcaacttaga agatttccac acacggtcaa taggcggctc agtatgccaa ctgggtcatg 120

accaagcact tctgtctccc cggactgagt atcaataagc tgctcacgtg gctgaaggag 180

aaaacgttcg ttatccggcc aattacttcg agaagcttag taccaccatg gaggttgcgc 240

agtgtttcgt tccgcacaac cccagtgtag atcaggtcga tgagtcaccg cactcctcac 300

gggcgactgt ggcggtggct gcgctggcgg cctgcccatg gggcacccca tgggacgctt 360

caatactgac atggtgcgaa gagcctattg agctaattgg taatcctccg gcccctgaat 420

gcggctaatc ctaactgcgg agcagatacc ctcgagccag tgggcagtct gtcgtaatgg 480

gcaactctgc agcggaaccg actactttgg gtgtccgtgt ttccttttat tcctatattg 540

gctgcttatg gtgacaattg agagattgtt accatatagc tattggattg gccatccagt 600

gacaaacaga gcgattatct acttatttgt tggctttgta ccattaaatc ttggagtcct 660

taagaccctc aacttgatta tacaatttaa ctctgcgaa 699

<210> 98

<211> 563

<212> DNA

<213> Echovirus E20 (HAI/2016-23077B)

<400> 98

cccccggact gagtatcaat aagctgctta cgtggctgaa ggagaaaacg ttcgttatcc 60

ggccaattac ttcgagaagc ttagtaccac catggaggtt gcgcagtgtt tcgttccgca 120

caaccccagt gtagatcagg tcgatgagtc accgcactcc tcacgggcga ccgtggcggt 180

ggctgcgctg gcggcctgcc catggggcaa cccatgggac gcttcaatac tgacatggtg 240

cgaagagcct attgagctaa ttggtagtcc tccggcccct gaatgcggct aatcctaact 300

gcggagcaga tacccacgag ccagtgggca gtctgtcgta atgggtaact ctgcagcgga 360

accgactact ttgggtgtcc gtgtttcctt ttattcctac attggctgct tatggtgaca 420

attgagagat tgttaccata tagctattgg attggccgtc cagtgacaaa cagagcgatt 480

atctatctat ttgttggttt cgtaccatta aaccttagag tcctaaagac cctcaatttg 540

attatacaat taaactctgc gaa 563

<210> 99

<211> 670

<212> DNA

<213> Echovirus E20 (strain HAI/2017-23083C)

<400> 99

gtgctcctgt tttacgcacc ctcccccgac agcaacttag aagatttcca cacacggtca 60

ataggcggct cagtatgcca actgggtcat gaccaagcac ttctgtctcc ccggactgag 120

tatcaataag ctgctcacgt ggctgaagga gaaaacgttc gttatccggc caattacttc 180

gagaagctta gtaccaccat ggaggttgcg cagtgtttcg ttccgcacaa ccccagtgta 240

gatcaggtcg atgagtcacc gcactcctca cgggcgactg tggcggtggc tgcgctggcg 300

gcctgcccat ggggcacccc atgggacgct tcaatactga catggtgcga agagcctatt 360

gagctaattg gtaatcctcc ggcccctgaa tgcggctaat cctaactgcg gagcagatac 420

cctcgagcca gtgggcagtc tgtcgtaatg ggcaactctg cagcggaacc gactactttg 480

ggtgtccgtg tttcctttta ttcctatatt ggctgcttat ggtgacaatt gagagattgt 540

taccatatag ctattggatt ggccatccag tgacaaacag agcgattatc tacttatttg 600

ttggctttgt accattaaat cttggagtcc ttaagaccct caacttgatt atacaattta 660

actctgcgaa 670

<210> 100

<211> 744

<212> DNA

<213> Echovirus E20 (strain KM-EV20-2010)

<400> 100

ttaaaacagc ctgtgggttg ttcccacccg cagggcccac ggggcgctag cacactggta 60

ttaaggtacc cttgtgcgcc tgttttatac acccctcccc ctaatgtaac ttagaagcat 120

gacttaagtg gtcaatagat agctcagtaa tccaactgag ttccgaccaa gtacttctgt 180

taccccggac cgagtatcaa taagctgttc acgcggctga aggagaaagc gttcgttatc 240

cggccaatta cttcgagaaa cccagtatca ccatgaaagt tgcgcagtgt ttcgctccgc 300

acaaccccag tgtagatcag gtcgatgagt caccacagtc ctcacgggcg accgtggtgg 360

tggctgcgtt ggcggcctgc ccatggggca acccatggga cgcttcaata ctgacatggt 420

gtgaagagtc tattgagctg gatggtatcc ctccggcccc tgaatgcggc taatcctagc 480

tgcggagcag acgctcgcat gccaacgggt agtctgtcgt accgggtaac tctgcagcgg 540

aaccgactac tttgggtgtc cgtgtttcct tttatcctta tatcggctgc ttatggtgtc 600

aattgaaaga ttgttaccat atagctaatg gattggccat ccgataacaa atagagtgat 660

tatctagttt tttgttggat ttgttccatt gaactacaag gtattgaaca cccttaattt 720

cattttaaca ctcaacacaa caaa 744

<210> 101

<211> 742

<212> DNA

<213> Echovirus E20 (strain JV-1)

<400> 101

ttaaaacagc ctgtgggttg ttcccaccca cagggcccat tgggcgctag cactctggta 60

ttacggtacc tttgtgcgcc tgttttatgt cccctccccc aatcgcaact tagaagcaac 120

acacactgat caacagtaag cgtggcatac cagccacgtt ttgatcaagc acttctgtta 180

ccccggactg agtatcaata gactgctcac gcggttgaag gagaaagcgt tcgttatccg 240

gccaactact tcgagaaacc tagtaacacc gtgaaagttg cagagtgttt cgctcagcac 300

taccccagtg tagatcaggt cgatgagtca ccgcattccc cacgggcgac cgtggcggtg 360

gctgcgctgg cggcctgcct acggggaaac ccgtaggacg ctctaataca gacatggtgc 420

gaagagtcta ttgagctagt tggtagtcct ccggcccctg aatgcggcta atcctaactg 480

cggagcacac acccccaagc cagggggcag tgtgtcgtaa cgggtaactc tgcagcggaa 540

ccgactactt tgggtgtccg tgtttcattt tattcctatg ctggctgctt atggtgacaa 600

ttgacagatt gttaccatat agctattgga ttggccatcc ggtgactaat agagccatta 660

tataccactt tgttgggttt ataccactca acttgaaaga ggtcaaaaca ctacagctca 720

tcattaaatt gaacacaaca aa 742

<210> 102

<211> 744

<212> DNA

<213> Echovirus E21 (strain 553/YN/CHN/2013)

<400> 102

ttaaaacagc ctgtgggttg cacccactca cagggcccat tgggcgctag cacaccggta 60

tcctggtacc cttgtgcgcc tgttttatcc acccttcccc cattgtaact tagaagttaa 120

tcaaacaagg tcgataggtg gctcagtaat ccaactgagt cttgaccaag cactactgtc 180

tccccggact gagtaccaat acgctgctaa cgtggctgaa ggggaaaacg ttcgttatcc 240

ggccaattac ttcgagaaac ctagtatcac catgaaggtt gcgcagcgtt tcgctccgca 300

cgacccctgt gtagatcagg ccgatgagtc accgcactcc ccacaggcga ctgtggcggt 360

ggctgcgctg gcggcctgcc catggggcaa cccatgggac gcttcaatac tgacatggtg 420

cgaagagtct attgagctaa ttggtagtcc tccggcccct gaatgcggct aatcctaact 480

gtggagcaga cacccacacg ccagtgggca gtctgtcgta acgggcaact ccgcagcgga 540

accgactact ttgggtgtcc gtgtttccat tttatttcta tgctggctgc ttatggtgac 600

aattgaaaga ttgttaccat atagctattg gattggccat ccggtgacaa acagagcaat 660

tgtctactta tttgttggat tcataccact ggacaacgca gtctatcata ctttgtgtta 720

catcattaaa ctgaacacag caaa 744

<210> 103

<211> 741

<212> DNA

<213> Echovirus E21 (strain Farina)

<400> 103

ttaaaacagc ctgtgggttg ttcccaccca cagggcccac cgggcgctag cacactggta 60

tcgcggtacc tttgtgcgcc tgttttattt accctgccct gatgtaactt agaagcatga 120

caccaacgat caatagcagg ctcaatgcac caattgggtc aagatcaagc acttctgtta 180

ccccggactg agtatcaata agctgctagc gcggctgaag gagaaaaagt tcgttacccg 240

gccagctact tcgagaaacc tagtatcacc atgaaagttg cgtggcgttt cgctccgcac 300

aaccccagtg tagatcaggt cgatgagtca ccgcattccc cacgggcgac cgtggcggtg 360

gctgcgttgg cggcctgccc gtggagcaat ccatgggacg ctataataca gacatggtgt 420

gaagagtcta ttgagctagc tggtagtcct ccggcccctg aatgcggcta atcctaactg 480

cggagcaggt acccacgaac cagtgggcag tctgtcgtaa tgggcaactc cgcagcggaa 540

ccgactactt tgggtgtccg tgtttccttt tatttcaact tggctgctta tggtgacaat 600

tgagagattg ttaccatata gctattggat tggccatccg gtgactaaca gagctatcat 660

ataccttttt attggctttg tgccacttac tctgaaagag gtcaagactc tgcattatat 720

catactattg aatgctataa a 741

<210> 104

<211> 721

<212> DNA

<213> Echovirus E24 (strain VEN/2018-23086)

<400> 104

cccacccata gggcccactg ggcgctagca cactggtatc acggtacctt tgtgcgcctg 60

ttttatttac cctcccccaa ctgaaaatta gaagccaaac tcaaacggtc aataggaaac 120

ccagtgaacc aactgggctg cgaccaagca cttctgttac cccggactga gtatcaatag 180

gctgctcgcg cggctgaagg agaaaacgtt cgttacccgg ccaattactt cgagaaacct 240

agtaccacca tgaaagttgc gaaacgtttc actccgcaca accccagtgt agatcgggtc 300

gatgagtcac cgcattcccc acgggtgacc gtggcggtgg ctgcgttggc ggcccgccca 360

tggggcaacc catgggacgc ttcaatactg acatggtgtg aagagtctat tgagctaatt 420

ggtagtcctc cggcccctga atgcggctaa tcctaactgc ggatcagaca cccacacgcc 480

agtgggcagt ctgtcgtaat gggcaactct gcagcggaac cgactacttt gggtgtccgt 540

gtttcttttt atccttatac tggctgctta tggtgacaat tgaaagattg ttaccatata 600

gctattggat tggccatccg gtatctaaca gagctattat ttacttattt gttggtttcg 660

tacctctcag tatcagagtt ttgagaacac ttaacttcat actactgcta aacactgcaa 720

g 721

<210> 105

<211> 746

<212> DNA

<213> Echovirus E24 (isolate PZ18G/JS/20120703)

<400> 105

tttaaaaaca agcctgtggg ttgctcccac ccacagggcc cactgggcgc tagcacactg 60

gtatcacggt acctttgtgc gcctgtttta tccgcccccc ccccttgtaa tttagaagat 120

tcgcacagac ggtcgacaga cactccagca caccagctgg acactgacca agcactcctg 180

ttaccccgga ctgagtatca ataggctgcc cacgcggctg aaggagaaaa cgttcgttac 240

ccggccaatt acttcgagaa acttagtaac gccatgaaag ttgcgcagtg tttcgctccg 300

cacaacccca gtgtagatca ggtcgatgag tcaccgcttt ccccacgggc gaccgtggcg 360

gtggctgcgt tggcggcctg cccatggggt aacccatggg acgcttcaat actgacatgg 420

tgcgaagagt ctattgagct aattggtagt cctccggccc ctgaatgcgg ctaatcctaa 480

ctgcggagca gatactcaca agccagtgag ctgtctgtcg taacgggcaa ctctgcagcg 540

gaaccgacta ctttgggtgt ccgtgtttcc tctttattct tgatttggct gcttatggtg 600

acaattgaaa aattgttacc gtatagctat tggattggcc atccagtgac aaatagagca 660

ataatttatt tgttcattgg ctttgtacca ttaaaccata aggttttcaa cactcttcaa 720

ttcattattg cacttaactc atcaaa 746

<210> 106

<211> 742

<212> DNA

<213> Echovirus E24 (strain DeCamp)

<400> 106

ttaaaacagc ctgtgggttg cacccaccca cagggcccac agggcgctag cactctggta 60

tcacggtacc tttgtgcgcc tgttttatta ccccttcccc aattgaaaat tagaagcaat 120

gcacaccgat caacagcagg cgtggcgcac cagtcacgtc tcgatcaagc acttctgttt 180

ccccggaccg agtatcaata gactgctcac gcggttgaag gagaaagtgt tcgttatccg 240

gctaaccact tcgagaaacc cagtaacacc atgaaagttg cagggtgttt cgctcagcac 300

ttccccagtg tagatcaggt cgatgagtca ccgcgttccc cacgggcgac cgtggcggtg 360

gctgcgttgg cggcctgcct atgggttaac ccataggacg ctctaataca gacatggtgc 420

gaagagttta ttgagctggt tagtatccct ccggcccctg aatgcggcta atcctaactg 480

cggagcacgt gcctccaatc cagggggttg catgtcgtaa cgggtaactc tgcagcggaa 540

ccgactactt tgggtgtccg tgtttccttt tattcttata ctggctgctt atggtgacaa 600

tcgaggaatt gttaccatat agctattgga ttggccatcc ggtgtctaac agagcgatta 660

tatacctctt tgttggattt atgcagctca ataccaccaa ctttaacaca ttgaaatata 720

tcttaaagtt aaacacagca aa 742

<210> 107

<211> 744

<212> DNA

<213> Echovirus E25 (strain USA/2016-19521)

<400> 107

ttaaaccagc ctgtgggttg ttcccaccca cagggcccac tgggcgctag cacactggta 60

tcccggtacc tttgtgcgcc tgttttaatc accccccccc caaacgtaac ttagaagttt 120

ataactcatg gtcaataggc gactcagcat accagctggg tcttgaccaa gcactcctgt 180

aaccccggac cgagtatcaa taggctgttt gcacggctga aggagaaaac gtccgttatc 240

cgaccaatta cttcgagaaa cctagtacca ccgtgaaggt tgcgcagtgt ttcgctccgc 300

acaaccccag tgtagatcag gtcgatgagt caccgcactc ctcacgggcg accgtggcgg 360

tggctgcgct ggcggcctgc ccatggggca acccatggga cgcttcaata ctgacatggt 420

gtgaagagtc tattgagcta attggtagtc ctccggcccc tgaatgcggc taatcctaac 480

tgtggagcag atacccacac gccagtgggc agtctgtcgt aacgggcaac tctgcagcgg 540

aaccgactac tttgggtgtc cgtgtttcct tttattctta tactggctgc ttatggtgac 600

aattgagaga ttgttaccat atagctactg gattggccat ccaataacaa acagagcaat 660

tgtttatctt tttgttggtt ttgtaccatt aaattacaag gttttgaaaa ccctcaaatt 720

ggtcatactg tttaatacag caaa 744

<210> 108

<211> 579

<212> DNA

<213> Echovirus E25 (strain USA/2018-23126)

<400> 108

ggtcaagcac ttctgtttcc ccggaccaag tatcaatagg ctgctcacgc ggctgaagga 60

gaaagtgttc gttatccggc caattacttc gagaaaccta gtaccaccat ggaagttgcg 120

cagcgtttcg ctccgcacga ccccggtgta gatcaggccg atgagtcacc gcactcccca 180

cgggtgaccg tggcggtggc tgcgctggcg gcctgctcat ggggcaaccc atgggacgct 240

tcaatactga catggtgtga agagtctatt gagctaattg gtagtcctcc ggcccctgaa 300

tgcggctaat cccaaccgcg gagcggattc ccacactcca gtgggcagtc tgtcgtaacg 360

ggcaactctg cggcggaacc gactactttg ggtgtccgtg tttcatttta ttccttattg 420

gctgcttatg gtgacaattg aaagattgtt gccatatagc tattggattg gccatccagt 480

gacaaacaga gcgattgtct atctatttgt tggtttcata ccattaaatt tcaaagtttt 540

aaagactatc aacttgatta tattgcttaa tacagcaaa 579

<210> 109

<211> 571

<212> DNA

<213> Echovirus E25 (strain 10-4339-2)

<400> 109

cttctgttat cccggactga gtatcaataa gctgcttgcg cggctgaagg agaaaacgtt 60

cgttatccgg ccaattactt cgagaaacct agtaccacca tgaaggttgc gcggcgtttc 120

gctccacaca accccagtgt agatcaggcc gatgagtcac cgcactcccc acgggcgacc 180

gtggcggtgg ctgcgttggc ggcctgccca tggggcaacc catgggacgc ttcaatactg 240

acatggtgtg aagagtctat tgagctaatt ggtagtcctc cggcccctga atgcggctaa 300

tcctaactgc ggagcagata cccacatgcc agtgggcagt ctgtcgtaat gggtaactct 360

gcagcggaac cgactacttt gggtgtccgt gtttcctttt attctaatat tggctgctta 420

tggtgacaat tgagagattg ttgccatata gctattggat tggccatcca gtgacaaaca 480

gagcaattgt ttatctattt gttggtttca taccattaaa tcttaaagct ttaagaaccc 540

tcaactttat tatacaactt aattcagtac a 571

<210> 110

<211> 643

<212> DNA

<213> Echovirus E25 (strain USA/CA/RGDS-2017-1010)

<400> 110

aagcgtaact tagaagctta cctcacgtgg tcaataggcg gctcagtaca ccaactgggt 60

cttgaccaag cacttctgtt accccggacc gagtatcaat aggctgccca cgcggctgaa 120

ggagaaaacg ttcgttaccc ggccaattac ttcgagaaac ctagtatcac catggaagtt 180

gcgcggcgtt tcgctccaca caaccccagt gtagatcagg ctgatgagtc accgcactcc 240

tcacgggcga ccgtggcggt ggctgcgttg gcggcctgcc catggggcaa cccatgggac 300

gcctcaatac tgacatggtg tgaagagtct attgagctaa ttggtagtcc tccggcccct 360

gaatgcggct aatcctaact gcggagcaga cactcacaag ccagtgggcg gtctgtcgta 420

acgggcaact ctgcagcgga accgactact ttgggtgtcc gtgtttcctt ttattcctac 480

attggctgct tatggtgaca attgagagat tgttaccata tagctattgg attggccatc 540

cggtgacaaa cagagcgatt gtctattcat ttgtcggttt tgtaccatta aactccaaag 600

tcttgaaaac cctcaaccta atcatcctac ttaactcagt aaa 643

<210> 111

<211> 571

<212> DNA

<213> Echovirus E25 (isolate NSW-V07-2007-ECHO25)

<400> 111

cttctgtttc cccggactga gtatcaataa gctgctcgcg cggctgaaag agaaaatgtt 60

cgttatccgg ccaattactt cgagaaaccc agtatcacca tggaggttgc gcagcgtttc 120

gctccgcacg accccagtgt agatcaggcc gatgagtcac cgcattcctc acgggcgacc 180

gtggcggtgg ctgcgttggc ggcctgccca tggggcaacc catgggacgc ttcaatactg 240

acatggtgtg aagagtctat tgagctaatt ggtagtcctc cggcccctga atgcggctaa 300

tcccaactgc ggagcagata ctcacatgcc agtgagcagt ctgtcgtaac gggcaactct 360

gcagcggaac cgactacttt gggtgtccgt gtttcctttt attcttatat tggctgctta 420

tggtgacaat tgagagattg ttaccatata gctattggat tggccatcca gtgacaaaca 480

gggcaatagt ttatctattt gttggtttta taccactgaa tttcaaggtt ttaaaaaccc 540

ttaacttggt cttactactc aattcagtaa g 571

<210> 112

<211> 571

<212> DNA

<213> Echovirus E25 (isolate NSW-V08-2008-ECHO25)

<400> 112

cttctgtttc cccggactga gtatcaataa gctgctcgcg cggctgaagg agaaaacgtt 60

cgtcacccgg ccaattactt cgagaaacct agtaccacca tggaggttgc gcagcgtttc 120

gctccacaca accccagtgt agatcaggtc gatgagtcac cgcactcctc acgggcgacc 180

gtggcggtgg ctgcgttggc ggcctgccca tggggcaacc catgggacgc ttcaatactg 240

acatggtgcg aagagtctat tgagctaatt ggtagtcctc cggcccctga atgcggctaa 300

tcctaactgc ggagcagata cccacatgcc agtgggcagt ctgtcgtaat gggtaactct 360

gcagcggaac cgactacttt gggtgtccgt gtttcctttt attcctacat tggctgctta 420

tggtgacaat tgagagattg ttaccatata gctattggat tggccatcca gtgacaaaca 480

gagcaattgt ttatctattt gttggtttca tatcactgaa tttcaaagtg ttaaaaaccc 540

ttaacctgat catactactt aattcagcaa a 571

<210> 113

<211> 276

<212> DNA

<213> Echovirus E25 (isolate NSW-V09-2008-ECHO25)

<400> 113

gctaatccta actgcggagc agatacccac atgccagtgg gcagtctgtc gtaatgggta 60

actctgcagc ggaaccgact actttgggtg tccgtgtttc cttttattcc tacattggct 120

gcttatggtg acaattgaga gattgttacc atatagctat tggattggcc atccagtgac 180

aaacagagca attgtttatc tatttgttgg tttcatatca ctgaatttca aagtgttaaa 240

aacccttaac ctgatcatac tacttaattc agcaaa 276

<210> 114

<211> 276

<212> DNA

<213> Echovirus E25 (isolate NSW-V58-2010-ECHO25)

<400> 114

gctaatccca actgcggggc agatacccac atgccagtgg gcagtctgtc gtaacgggca 60

actctgcagc ggaaccgact actttgggtg tccgtgtttc cttttattat tatattggct 120

gcttatggtg acaattgaga gattgttacc atatagctat tggattggcc atccggtatc 180

aaatagagca atcatttatc tatttgttgg atttgttcca ttaaatttta aagttttgca 240

aactctcaat ttaattacat tacttaattc agtgaa 276

<210> 115

<211> 711

<212> DNA

<213> Echovirus E25 (strain 61241-70868)

<400> 115

agggcccact gggcgctagc actctggtat tgcggtacct ttgtgcgcct gttttatggc 60

ccccccccag ttggaactta gaagtgaagc ctctcgacca atagtgggcg tggcacacca 120

gccacgtctt ggtcaagcac atctgtttcc ccggactgag tatcaataaa ctgctcgcgc 180

ggttgaagga gaaaacgttc gttacccggc taactacttc gggaaaccca gtagtatcat 240

ggaaattgcg aggcgtttcg ctcagcactc cccccgtgca gatcaggtcg atgagtcact 300

gcacccccca cgggtgaccg tggcagtggc tgcgttggcg gcctgcctat ggggtgaccc 360

ataggacgct tcaaaatgga catggtgcga agagtctact gagctagtta gtagtcctcc 420

ggcccctgaa tgcggcaaat cctaaccgcg gagcaagtgt ccacaaacca gtgggttgct 480

tgtcgtaatg ggtaactctg cggcggaacc gactactttg ggtgtccgtg tttcctttta 540

tcttttatta tggctgctta tggtgacaat tgagagattg ttaccatata gctattggat 600

tggccatccg gtgaacaaca gagcgatcat ttacctcttt gttggtttcg ttccattgag 660

tcccaaagtt tttacgacac tcaaatttat tctcctactt aacaaagcaa a 711

<210> 116

<211> 744

<212> DNA

<213> Echovirus E25 (strain E25/ZE-wly/Zhejiang/CHN/2005)

<400> 116

tttaaaacag cctgtgggtt gttcccaccc acagggccca ttgggcgcca gcacactggt 60

atttcggtac ctttgtgcgc ctgttttatc cacccctccc ccaaacgtaa cttagaagct 120

caacacacat ggtcagtagg tggcccagta agccaaccgg gtcttgacca agcacttctg 180

ttaccccgga ccgagtatca ataggctgct cacgcggctg aaggagaaag tgttcgttat 240

ccggccaatt acttcgagaa acctagtacc accatggaag ttgcgcagcg tttcgctccg 300

cacgacccca gtgtagatca ggccgatgag tcaccgcact cctcacgggc gaccgtggcg 360

gtggctgcgc tggcggcctg cccatggggc aacccatggg acgcttcaat gctgacatgg 420

tgcgaagagt ctattgagct aattggtaat cctccggccc ctgaatgcgg ctaatcctaa 480

ctgcggagca gatatccaca cgccagtggg cagtctgtcg taacgggcaa ctctgcagcg 540

gaaccgacta ctttgggtgt ccgtgtttct ctttattctt tattggctgc ttatggtgac 600

aattgaaaga ttgttgccat atagctattg gattggccat ccagtgacaa acagagcgat 660

cgtttatcta tttgttggtt tcataccatt aaattttaaa gttttgaaaa ctatcaactt 720

gatcatacta cttaatacag caaa 744

<210> 117

<211> 733

<212> DNA

<213> Echovirus E25 (isolate Jena/AN1380/10)

<400> 117

ctgtgggttg ttcccaccca cagggcccac tgggcgttag cactctggta ttgcggtacc 60

tttgtgcgcc tgttttatgg cccccccccc agttggaact tagaagtgaa gcttctcgac 120

caatagtggg cgtggcacac cagtcacgtc ttggtcaagc acatctgttt ccccggactg 180

agtatcaata aactgcttgc gcggttgaag gagaaaacgt tcgttacccg gctaactact 240

tcgggaagcc cagtagtatc atggaaattg cgaagcgttt cgctcagcac tccccccgtg 300

tagatcaggt cgatgagtca ctgcaccccc cacgggtgac cgtggcagtg gctgcgttgg 360

cggcctgcct atggggtgac ccataggacg cttcaaaatg gacatggtgc gaagagccta 420

ctgagctagt tagtagtcct ccggcccctg aatgcggcta atcctaaccg cggagcaagc 480

gtccacaaac cagtgggttg cttgtcgtaa tgggtaactc tgcggcggaa ccgactactt 540

tgggtgtccg tgtttccttt tatcttttat tgtggctgct tatggtgaca attgagagat 600

tgttaccata tagctattgg attggccatc cggtgaacaa cagagcggtc atttacctct 660

ttgttggttt cgttccattg agtcccaaag tttttacgac actcaaattt attctcctac 720

ttaacaaagc aaa 733

<210> 118

<211> 743

<212> DNA

<213> Echovirus E25 (strain XM0297)

<400> 118

ttaaaacagc ctgtgggttg ttcccaccca tagggcccac tgggcgctag cacactggta 60

ttgtggtacc tttgtgcgcc tgttttatct acccctcccc caaacgtgac ttagaagctc 120

aacacatgtg gtcagcaggt ggcccagtat accaactggg tcttgatcaa gcacttctgt 180

taccccggac cgagtatcaa taggctgctc acgcggctga aggagaaagt gttcgttatc 240

cggccaatta ctacgagaaa cctagtacca ccatggaagt tgcgcggcgt ttcgctccgc 300

acaaccccag tgtagatcag gccgatgagt caccgcactc ctcacgggcg accgtggcgg 360

tggctgcgct ggcggcctgc ccatggggca acccatggga cgcttcaata ccgacatggt 420

gtgaagagtc tattgtgcta attggtaatc ctccggcccc tgaatgcggc taatcccaac 480

tgcggagcag atacccacat gccagtgggc agtctgtcgt aacgggtaac tctgcagcgg 540

aaccgactac tttgggtgtc cgtgtttctt tttattcttt attggctgct tatggtgaca 600

attgagagat tgttgccata tagctattgg attggccatc cggtgacaaa cagaacaata 660

gtttatctat ttgttggttt cataccatta aattttaaag tattaaagac tatcaacttg 720

atcatactac ttaatacagc aaa 743

<210> 119

<211> 743

<212> DNA

<213> Echovirus E25 (strain E25/2010/CHN/BJ)

<400> 119

ttaaaacagc ctgtgggttg ttcccaccca tagggcccac tgggcgctag cacactggta 60

ttacggtacc tttgtgcgcc tgttttattc acccctctcc caaacgtaac ttagaagctc 120

aacacatgtg gtcagtaggc agcccagtat gccaactggg tcttgaccaa gcacttctgt 180

taccccggac tgagtatcaa taggctgctc acgcggctga aggagaaagt gttcgtcacc 240

cggccaatta cttcgagaaa cctagtatca ccatggaagt tgcgcagcgt ttcgctccgc 300

acgaccccag tgtagatcag gccgatgagt caccgcactc ctcacgggcg accgtggcgg 360

tggctgcgct ggcggcctgc ccatgaggca actcatggga cgcttcaata ctgacatggt 420

gtgaagagtc tattgagcta attggtagtc ctccggcccc tgaatgcggc taatcctaac 480

cgcagagcag atacccacac gccagtgggc agtctgtcgt aacgggtaac tctgcggcgg 540

aaccgactac tttgggtgtc cgtgtttctt tttattcttt attggctgct tatggtgaca 600

attgaaagat tgttgccata tagctattgg attggccatc cagtgacaaa cagagcgact 660

gtttatctat ttgttggttt cataccatta aattttaaag tattaaagac tatcaacttg 720

atcatactac ttaatacagc aaa 743

<210> 120

<211> 743

<212> DNA

<213> Echovirus E25 (isolate E25SD2010CHN)

<400> 120

ttaaaaccag gctgtgggtt gttcccaccc acagggccca ctgggcgcta gcacactggt 60

attgcggtac ctttgtgcgc ctgttttata taccctttcc caaatgtaac ttagaagttt 120

aaaccacatg gtcagtagac accccagtac accaactggg tttggaccaa gcacttctgt 180

taccccggac tgagtatcaa taagctgctc gcgcggctga aggagaaaac gttcgttatc 240

cgatcaatta cttcgagaaa cctagtaaca ccatgaaagt tgcgcggcgt ttcgttccgc 300

acaaccccag tgtagatcag gtcgatgagt caccgcattc cccacgggcg accgtggcgg 360

tggctgcgtt ggcggcctgc ccatggggta acccatggga cgcttcaata ctgacatggt 420

gcgaagagtc tattgagcta attggtattc ctccggcccc tgaatgcggc taatcctaac 480

tgcggagcag atacccacat accagtgggc agtctgtcgt aasgggcaac tctgcagcgg 540

aaccgactac tttgggtgtc cgtgtttcct tttattcttt attggctgct tatggtgaca 600

attgaaagat tgttgccata tagctattgg attggccatc cagtgacaaa cagagcgatt 660

gtttatctat ttgttggttt cataccatta aattttaaag tcttaaaaac tattaacttg 720

atcatactac ttaatacagc aaa 743

<210> 121

<211> 743

<212> DNA

<213> Echovirus E25 (strain HN-2)

<400> 121

ttaaaaccag gctgtgggtt gttcccaccc atagggccca ctgggcgcca gcactctggt 60

attccggtac ctttgtgcgc ctgttttatc cacccctccc ccaaacgtaa cttagaagct 120

caacacatgt ggtcagtagg tagcccagta tgccaactgg gtcttgacca agcacttctg 180

ttaccccgga ccaagtatca ataggctgct cacgcggctg aaggagaaag tgttcgttac 240

ccggccgatt acttcgagaa acctagtacc accatggaag ttgcgcagcg tttcgctccg 300

cacgacccca gtgtagatca ggccgatgag tcaccgcact cctcacgggc gaccgtggcg 360

gtggctgcgc tggcggcctg cccatggggc aacccatggg acgcttcaat actgacatgg 420

tgtgaagagt ctattgagct aattggtagt cctccggccc ctgaatgcgg ctaatcctaa 480

ccgcggagca gatacccaca cgccagtggg cagtctgtcg taacgggcaa ctctgcggcg 540

gaaccgacta ctttgggtgt ccgtgtttca ttttattctt tattggctgc ttatggtgac 600

aattgaaaga ttgttgccat atagctatgg attggccatc cagtgacaaa cagagcgatt 660

gtttatctat ttgttggttt cataccatta aattttaaag ttttaagaac tatcaacttg 720

atcatactac ttaatacaac aaa 743

<210> 122

<211> 741

<212> DNA

<213> Echovirus E25 (strain JV-4)

<400> 122

ttaaaacagc ctgtgggttg tacccaccca cagggcccac tgggcgctag cactctggta 60

ctacggtacc tttgtgtgcc tgttttatac ccctccccct actgaaactt agaagcaatt 120

cataccgatc aatagtgggc gtggcacacc agccgtgtct agatcaagca ctcctgtttc 180

cccggaccga gtatcaatag actgctcacg cggttgaagg agaaaacgtt cgttatccgg 240

ctaactactt cgaaaaacct agtaacacca tgaaagttgc ggagtgtttc actcagcact 300

tccccagtgt agatcaggtc gatgagtcac cgcattcctc acgggcgacc gtggcggtgg 360

ctgcgctggc ggcctgccta tggggtgacc cataggacgc tctaatacag acatggtgcg 420

aagagtctat tgagctagtt agtagtcctc cggcccctga atgcggataa tcctaactgt 480

ggagcagata cccacgaacc agtgggcagt ctgtcgtaac gggcaactcc gcagcggaac 540

cgactacttt gggtgtccgt gtttcctttt attccaaatc tggctgctta tggtgacaat 600

tgagagattg ttgccatata gctattggat tggccatccg gtgaataata gagcgataat 660

atatttgttt gttggattcg tgccacttag tctgaaagtt ttgagaacac tcaactacgt 720

tttattgctg aatagtgcaa g 741

<210> 123

<211> 741

<212> DNA

<213> Echovirus E26 (strain Coronel)

<400> 123

ttaaaacagc ctgtgggttg ttcccaccca cagggcccac tgggcgctag cacaccggta 60

tcacggtacc cttgtgcgcc tgttttatcc acctcccccg ttgtaactta gaagctcaat 120

acaaatggtc gatagccagc ccgatacgcc aattgggttt tgaccaagca cttctgttac 180

cccggactga gtaccaatag gctgccaaag cggctgaagg ggaaaacgtt cgttacccca 240

ccaactactt cgagaaacct agtaccacca tgaagattgc gcagcgtttc gctcagcaca 300

accccagtgt agatcaggtc gatgagtcac cgcattcccc acaggcgact gtggcggtgg 360

ctgcgttggc ggcctgccca tggggctacc catgggacac ttcaatatcg acatggtgcg 420

aagagtctat tgagctagtt ggtagtcctc cggcccctga atgcggataa tcctaactgc 480

ggagcaggca cccacaagcc agtgggcagc ctgtcgtaac gggcaactct gcagcggaac 540

cgactacttt gggtgtccgt gtttcttttt attcttacac tggctgctta tggtgacaat 600

tgaagaattg ttaccatata gctattggat tggccatcca gcgactaaca gagctattat 660

cttcttgttt gtggggtacg taccactcaa tttcacagta gtaaggactc tcaatttcat 720

tttgtgcttg aataaggcga a 741

<210> 124

<211> 538

<212> DNA

<213> Echovirus E27 (isolate ETH_P8/E27_2016)

<400> 124

gctcacgcgg ctgaaggaga aaacgttcgt tacccggcca attacttcga gaaacctagt 60

accactatga aggttgcgca gcgtttcgct ccgcacaacc ccagtgtaga tcaggccgat 120

gagtcaccgc atccctcacg ggcgaccgtg gcggtggctg cgttggcggc ctgcccatgg 180

ggcaacccat gggacgcttc aatactgaca tggtgcgaag agtctattga gctaattggt 240

agtcctccgg cccctgaatg cggctaatcc taactgcgga gcagataccc gcgcaccagc 300

gggcagtctg tcgtaatggg caactctgca gcggaaccga ctactttggg tgtccgtgtt 360

tccttttatt cttatactgg ctgcttatgg tgacaattga gagattgtta ccatatagct 420

attggattgg ccacccggtg actaacagag cgattatcta ccaatttgtt ggttttgtgc 480

cattggactt ttcaacccta agaaccctca atttcatatt agtgcttaac acagcaaa 538

<210> 125

<211> 744

<212> DNA

<213> Echovirus E27 (strain Bacon)

<400> 125

ttaaaacagc ctgtgggttg tacccaccta cagggcccac tgggcgtcag cacactggta 60

tcccggtacc tttgtgcgcc tgttttatat tccccacccc acgttgtaac ttagaagcaa 120

tgcaatttcg gtcagtagta gacgcggcaa cccagtcgtg tttcgaccaa gcacttctgt 180

aaccccggac cgagtatcaa taagctgctc gcgcggctga aggagaaacc gttcgttacc 240

cggctaacta cttcgagaag cctactaaca ccatgaacgt tgcagagtgt ttcactcagc 300

acttcccccg tgtagatcag gtcgatgagt caccgcgatc cccacgggcg accgtggcgg 360

tggctgcgtt ggcggcctgc ctgtggggca acccacagga cgctctaata cagacatggt 420

gcgaagagcc tattgagcta gttagtagtc ctccggcccc tgaatgcggc taatcctaac 480

tgcggagcac atgcccacaa accagtgggt ggtgtgtcgt aacgggcaac tctgcagcgg 540

aaccgactac tttgggtgtc cgtgtttctt tttattctca tactggctgc ttatggtgac 600

aattgaaaga ttgttgccat atagctattg gattagccat ccggtgtcaa acagagcaat 660

tatttaccta ttcgttggtt ttgcaccctt gaattttaag gttcttaaaa cacttaatct 720

catattaaaa ctcaattcag caaa 744

<210> 126

<211> 576

<212> DNA

<213> Echovirus E29 (strain HAI/2016-23048B)

<400> 126

aagcacttct gttgccccgg actgagtatc aataggctgc tcacgcggct gaaggagaaa 60

acgttcgtta cccggccaat tacttcgaga aacttagtac caccatgaag gttgcgcggc 120

gtttcgctcc acacaacccc agtgtagatc aggtcgatga gtcaccgcgt tccccacggg 180

cgaccgtggc ggtggctgcg ttggcggcct gcccatgggt caacccatgg gacgcttcaa 240

tactgacatg gtgtgaagag tctattgagc taattggtat tcctccggcc cctgaatgcg 300

gctaatccta actgcggagc agatacccac atgccagtgg gcagtctgtc gtaacgggca 360

actctgcagc ggaaccgact actttgggtg tccgtgtttc cttttatcct tatactggct 420

gcttatggtg acaattgaga gattgttacc atatagctat tggattggcc atccggtgac 480

aagcagagca attatttatt tgtttgttgg cttcgtgcca ttaaatagta aagttctaat 540

aaccctcaac ctcatcttgt tacttaatag tgcaaa 576

<210> 127

<211> 743

<212> DNA

<213> Echovirus E29 (strain JV-10)

<400> 127

ataaaacagc ctgtgggttg tacccaccca cagggcccac tgggcgctag cactctggta 60

ttacggtacc cttgtgcgcc tgttttatct tcccttccct cagtgcaact tagaagtata 120

atcttaatga tcagtaggtg gctcagcatg ccagctgggt cttgatcaag cacttctgtt 180

accccggact gagtatcaat aagctgctcg cgcggctgaa ggagaaagcg ttcgttaccc 240

ggccaattac ttcgagaaac ctagtaccac catggaagtt gcgcggcgtt tcgctccgca 300

caatcccagt gtagatcagg tcgatgagtc accgcattcc ccacgggcga ccgtggcggt 360

ggctgcgttg gcggcctgcc catggggcaa cccatgggac gcttcaatac tgacatggtg 420

cgaagagtct attgagctaa ttggtagtcc tccggcccct gaatgcggct aatcctaact 480

gcggagcaga tacccacaag ccagtgggcg atctgtcgta acgggcaact ctgcagcgga 540

accgactact ttgggtgtcc gtgtttcttt ttattcctat actggctgct tatggtgaca 600

attgagaaat tgttaccata tagctattgg attggccatc cggtgactaa cagagcaatt 660

gtatacctgt ttgttggttt tgtgccattg aacttcaaag ttttaaaaac actcaattta 720

ataattctac ttaatacaac aag 743

<210> 128

<211> 768

<212> DNA

<213> Echovirus E30 (isolate E30/TO/BR/032)

<400> 128

ggacaggata ccagtgtgct tgcgcccagt gggccctgtg ggttgttccc acccacaggg 60

cccactgggc gctagcacac tggtatcacg gtacctttgt gcgcctgttt tatccaccct 120

taccccaaag caacttagaa gcacgcacca tgcggccgat aggcggctca gtacgccaac 180

tgagtcttgg ccaagcactt ctgttacccc gggccgagta tcaataagct gcttgtgtgg 240

ctgaaggaga aagcgtccgt tacccggcca actacttcga gaaacctagt accaccatgg 300

atgttgcgtg gcgtttcgct ccgcacaacc ccagtgtaga tcaggtcgat gagtcaccgc 360

acccctcacg ggcgaccgtg gcggtggctg cgctggcggc ctgcctatgg ggcaacccat 420

aggacgcttc aatacggaca tggtgagaag agtctattga gctagttagt agtcctccgg 480

cccctgaatg cggctaatcc taactgcgga gcaggcgttc acaaaccagt ggatagcctg 540

tcgtaacggg caactctgca gcggaaccga ctactttggg tgtccgtgtt tccttttatt 600

cctatactgg ctgcttatgg tgacaattga tagattgtta ccatatagct gttggattgg 660

ccatccggtg actaatagag ctatcatcta cttgtttgtt ggtttcgtgc cactcaacta 720

caaagcctta caaaccctaa actttatttt gacactcaat acagcaag 768

<210> 129

<211> 743

<212> DNA

<213> Echovirus E30 (isolate TL12C/NM/CHN/2016)

<400> 129

ttaaaacagc ctgtgggttg ttcccacccg cagggcccac tgggcgctag cacactggta 60

tcacggtact tttgtgcgcc tgttttatcc acccttcccc gaatgaaact tagaagtttg 120

aattaaacgg tcaataggaa acccagtaca ccaactgggt catgaccaag cacttctgtg 180

accccggacc gagtatcaat aagctactca cgtggctgaa ggagaaaacg ttcgttaccc 240

ggccaattac ttcgagaaac ttagtaccac catgaaagtt gcgcagcgtt tcgctccgca 300

cgaccccagt gtagatcagg ctgatgagtc accgcgttcc ccacgggtga ccgtggcggt 360

ggctgcgttg gcggcctgcc cttggggcaa cccaagggac gcttcaatac tgacatggtg 420

cgaagagtct attgagctaa ttggtagtcc tccggcccct gaatgcggct aatcccaact 480

gcggagcaga tacccacatg ccagtgggca gtctgtcgta atgggcaact ctgcagcgga 540

accgactact ttgggtgtcc gtgtttcctt tatttcttac actggctgct tatggtgaca 600

attgagagat tgttgccata tagctattgg attggccatc cggtgtctaa tagagcaatt 660

atctaccttt ttgttggatt tattccactc aactataaag tactcaaaac acttacatac 720

attgtagatc tcaacacaac aaa 743

<210> 130

<211> 743

<212> DNA

<213> Echovirus E30 (isolate TL7C/NM/CHN/2016)

<400> 130

ttaaaacagc ctgtgggttg ttcccacccg cagggcccac tgggcgctag cacactggta 60

tcacggtact tttgtgcgcc tgttttatcc acccttcccc aaatgaaact tagaagtttg 120

aattaaacgg tcaataggaa acccagtaca ccaactgggt catgaccagg cacctctgtg 180

accccggacc gagtatcaat aagctactca cgtggctgaa ggagaaaacg ttcgttaccc 240

ggccaattac ttcgagaaac ttagtaccac catgaaagtt gcgcagcgtt tcgctccgca 300

cgaccccagt gtagatcagg ctgatgagtc accgcgttcc ccacgggtga ccgtggcggt 360

ggctgcgttg gcggcctgcc cttggggcaa cccaagggac gcttcaatac tgacatggtg 420

cgaagagtct attgagctaa ttggtagtcc tccggcccct gaatgcggct aatcccaact 480

gcggagcaga tacccacatg ccagtgggca gtctgtcgta atgggcaact ctgcagcgga 540

accgactact ttgggtgtcc gtgtttcctt tatttcttac actggctgct tatggtgaca 600

attgagagat tgttgccata tagctattgg attggccatc cggtgtctaa cagagcaatt 660

atctaccttt ttgttggatt tattccactc aactataaag tactcaaaac acttacatac 720

attgtagatc tcaacacaac aaa 743

<210> 131

<211> 580

<212> DNA

<213> Echovirus E30 (strain USA/2018-23125)

<400> 131

tggtcaagca cttctgtttc ccggaccgag tatcaataag ctactcacgt ggctgaagga 60

gaaaacgttc gttacccgac caactacttc gagaaaccta gtaccaccat gaatgttgcg 120

cggcgtttcg ctccgcacta ccccagtgta gatcaggtcg atgagtcacc gcatccctca 180

cgggcgaccg tggcggtggc tgcgctggcg gcctgcccat gggacaaccc atgggacgct 240

tcaacacaga catggtgtga agagtctatt gagctagttg gtagtcctcc ggcccctgaa 300

tgcggctaat cctaactgcg gagcagatcc ccacaagcca gtgggcagtc tgtcgtaatg 360

ggcaactctg cagcggaacc gactactttg ggtgtccgtg tttcctttta ttctcatact 420

ggctgcttat ggtgacaatt gaaggattgt taccatatag ctattggatt ggccatccgg 480

tgtctaacag agcaataatt tatctttttg ttggattcgt cccacttaac tacagagcac 540

ttgcaacgct caattacatc atagatctca acctaacaaa 580

<210> 132

<211> 742

<212> DNA

<213> Echovirus E30 (Echo30/Hokkaido.JPN/21208/2017)

<400> 132

ttaaaacagc ctgtgggttg atcccaccca cagggcctat tgggcgctag cacactggta 60

tcacggtacc tttgtgcgcc tgttttaccc acccttcccc gaaagaaact tagaagctca 120

acttgtgtgg tcaacagcaa gctcagtacg ccaactgggc cacgaccaag cacttctgtt 180

accccggact gagtatcaat aagctgctcg cgtggctgaa ggagaaaacg ttcgttatcc 240

ggccaattac ttcaagaaac ccagtaccac catgaaagtt gcgcggcgtt tcgttccgca 300

caaccccagt gtagatcagg ccgatgagcc accgcgttcc ccacgggcga ccgtggcggt 360

ggctgcgttg gcggcctgcc catggggcaa cccatgggac gcttcaatac tgacatggtg 420

cgaagagtct attgagctaa ttggtagtcc tccggcccct gaatgcggct aatcctaacc 480

gcggagcaga tacccacatg ccagtgggca gtctgtcgta acgggcaact tcgcggcgga 540

accgactact ttgggtgtcc gtgtttcctt ttattttata ctggctgctt atggtgacaa 600

ttgagagatt gttaccatat agctattgga ttggccaacc ggtgtccaac agagcgatcg 660

tttacttgtt tgttggattc gtgcccttga actacaaagc actcgtaacg cttaaataca 720

ttatagacct caacacagca aa 742

<210> 133

<211> 398

<212> DNA

<213> Echovirus E30 (strain USA/2015/CA-RGDS-1046)

<400> 133

ggcgaccgtg gtggtggctg cgctggcggc ctgcccatgg ggcaacccat gggacgcttc 60

aatacagaca tggtgtgaag agtctattga gctagttggt agtcctccgg cccctgaatg 120

cggctaatcc taactgcgga gcagatcccc acacaccagt gggcagtctg tcgtaatggg 180

taactctgca gcggaaccga ctactttggg tgtccgtgtt tccttttatt cttatactgg 240

ctgcttatgg tgacaattga aagattgtta ccatatagct attggattgg ccatccagtg 300

tctaacagag caataattta tctttttgtt ggattcgtcc cacttgacta cagagcactt 360

gcaacgctca attacattat agatctcaac acaataaa 398

<210> 134

<211> 598

<212> DNA

<213> Echovirus E30 (strain USA/2017/CA-RGDS-1048)

<400> 134

gtacaccaac tgggccatga ccaagcactt ctgtgacccc ggaccgagta tcaataagct 60

gctcacgtgg ctgaaggaga aaacgttcgt tacccggcca attacttcga gaaacctagt 120

accgccatga aagttgcgca gcgtttcgct ccgcacgacc ccagtgtaga tcaggctgat 180

gagtcaccgc gttccccacg ggtgaccgtg gcggtggctg cgttggcggc ctgcccttgg 240

ggcaacccaa gggacgcttc aatactgaca tggtgcgaag agtctattga gctaattggt 300

agtcctccgg cccctgaatg cggctaatcc caactgcgga gcagataccc acataccagt 360

gggcagtctg tcgtaatggg caactctgca gcggaaccga ctactttggg tgtccgtgtt 420

tcctttattt ctcacactgg ctgcttatgg tgacaattga gagattgttg ccatatagct 480

attggattgg ccatccggtg tctaatagag caattatcta cctttttgtt ggatttatcc 540

cacttaacta taaagcactc aaaacactaa tatacattgt ggatcttaac acaacaaa 598

<210> 135

<211> 658

<212> DNA

<213> Echovirus E30 (isolate B001/USA/2016)

<400> 135

tatccaccct ctccccatag taacttagaa gtatgtttca tatggtcaat aggcggctca 60

gtatgccaac tgagtctcga ccaagcactt ctgttgcccc ggaccgagta tcaataagct 120

actcacgtgg ctgaaggaga aaacgttcgt tacccgacca actacttcga gaaacctagt 180

accaccatgg atgttgcgcg gcgtttcgct ccgcactacc ccagtgtaga tcaggtcgat 240

gagtcaccgc attcctcacg ggcgaccgtg gtggtggctg cgctggcggc ctgcccatgg 300

ggcaacccat gggacgcttc aatacagaca tggtgtgaag agtctattga gctagttggt 360

agtcctccgg cccctgaatg cggctaatcc taactgcgga gcagatcccc acacaccagt 420

gggcagtctg tcgtaatggg taactctgca gcggaaccga ctactttggg tgtccgtgtt 480

tccttttatt ctcatactgg ctgcttatgg tgacaattga aagattgtta ccatatagct 540

attggattgg ccatccagtg tctaacagag caataattta tctttttgtt ggattcgtcc 600

cacttgacta cagagcactt gcaacgctca attatattat agatctcaac acaacaag 658

<210> 136

<211> 576

<212> DNA

<213> Echovirus E30 (strain 16-I10)

<400> 136

gcctattggt actcggtccg gggtaacaga agtgcttggc caaggtggct gaaggggaaa 60

acgttcgtta accgactaat aacttcgaga aacctagtac caccatgaaa gttgcgcagt 120

gtttcgctcc acacaacccc agtgtagatc aggtcgatga gtcaccgcgt tccccacggg 180

tgaccgtggc ggtggctgcg ttggcggcct gcccatgggg caacccatgg gacgcttcaa 240

tactgacatg gtgcgaagag tctattgagc ttattggtag tcctccggcc cctgaatgcg 300

gctaatccta actgcggagc aggtacccac aagccagtgg gtggcctgtc gtaacgggca 360

actctgcagc ggaaccgact actttgggtg tccgtgtttc cttttattct taaattggct 420

gcttatggtg acaattgaaa ggttgttgcc atatagctat tggattggcc atccggtgtc 480

taatagagca attatctacc tttttgttgg attcattcca ctcaactata aaacactcaa 540

aacactcata tacattgtag gtctcaacac agcaaa 576

<210> 137

<211> 502

<212> DNA

<213> Echovirus E30 (strain 1-B4-TW)

<400> 137

gccaattact tcgagaaacc tagtaccacc atggaggttg cgcagcgttt cgctccgcac 60

agccccagtg cagatcaggc cgatgagtca ccgcattcct cacgggcgac cgtggcggtg 120

gctgcgttgg cggcctgccc atggggcaac ccatgggacg cttcaatact aacatggtgt 180

gaagagtcta ttgagctaat tggtagtcct ccggcccctg aatgcggcta atcctaactg 240

cggagcagat actcacatgc cagtgggcag tctgtcgtaa cgggcaactc tgcagcggaa 300

ccgactactt tgggtgtccg tgtttccttt tattcttata ctggctgctt atggtgacaa 360

ttgagaaatt gttaccatat agctattgga ttggccatcc agtgacaaac agagcaattg 420

tttacctatt tgtcggcttc ataccgttaa attttagagt tttgaagacc cttaacttga 480

ttatactatt caactctgcg aa 502

<210> 138

<211> 708

<212> DNA

<213> Echovirus E30 (strain 2002-59)

<400> 138

cccattgggc gctagcacac tggtattgcg gtacctttgt gcgcctgttt tatccaccct 60

tccctcaaat gaaacttaga agcttaactt gaatggtcaa caggaaaccc agtacgccaa 120

ctgggtcgcg accaagcact tctgtaaccc cggactgagt atcaataagc tgcttgcgtg 180

gctgaaagag aaaacgtccg ttacccggcc aattacttcg agaaacctag taccaccatg 240

gaggttgcgc agtgtttcgc tccgcacaac cccagtgcag atcgggccga tgagtcaccg 300

cgttccccac gggcgaccgt ggcggtggct gcgctggcgg cctgcccatg gggtaaccca 360

tgggacgctt caatactgac atggtgcgaa gagtctattg agctagttgg tagtcctccg 420

gcccctgaat gcggctaatc ccaactgcgg agcagatacc cacacgccag tgggcagtct 480

gtcgtaacgg gcaactctgc agcggaaccg actactttgg gtgtccgtgt ttccttttat 540

cttatactgg ctgcttatgg tgacaattga gagattgtta ccatatagct actggattgg 600

ccatccggtg tctaacagag cgatcattta tctgtttgtt ggatttgtgc ccttaaacct 660

caaagttctc aacactctta accttgtact acaatttaac tcagcaaa 708

<210> 139

<211> 743

<212> DNA

<213> Echovirus E30 (strain KM/A363/09)

<400> 139

ttaaaacagc ctgtgggttg aacccatcca cagggcccac tgggcgcaag cacactggta 60

tcacggtacc tttgtgcgcc tgttttatcc acccttcccc gaacgaaact tagaagtttg 120

acttaaacgg tcaatagaaa gcccagtaca ccaactgggt cgtgaccaag cacttctgtg 180

accccggact gagtatcaat aagctactca cgtggctgaa ggagaaaacg ttcgttatcc 240

ggccaattac ttcgagaaac ttagtaccac catgaaggtt gcgtagcgtt tcgctccgca 300

caaccccagt gtagatcagg tcgatgagtc accgcgttcc tcacgggcga ccgtggcggt 360

ggctgcgttg gcggcctgcc cttggggcaa cccaagggac gcttcaatac tgacatggtg 420

cgaagagtct attgagctaa ttggtagtcc tccggcccct gaatgcggct aatcctaact 480

gcggagcaga tacccacatg ccagtgggca gtctgtcgta atgggcaact ctgcagcgga 540

accgactact ttgggtgtcc gtgtttcctt ttattcttat actggctgct tatggtgaca 600

attgagaaat tgttaccata tagctattgg attggccatc cagtgtccaa tagagcaatc 660

atttaccttt tcgttggatt tattccactt gactacaaag cactcaaaac actcgaatac 720

attgtagacc tcaatacaac aaa 743

<210> 140

<211> 646

<212> DNA

<213> Echovirus E30 (isolate 1-MRS2013)

<400> 140

cccgaaagaa acttagaagc tcaacttgtg tggtcaatag taagatcagt acgccaactg 60

agccacgacc aacgtacttc tgttaccccg gactgagtat caataagctg ctcacgtggc 120

tgaaggagaa aacgtccgtt acccggccaa ttacttcgag aaacccagta ccaccatgaa 180

agttgcgcgg cgtttcgctc cgcacaaccc cagtgtagat cgggccgatg agtcaccgcg 240

ttccccacgg gcgaccgtgg cggtggctgc gttggcggcc tgcccatgga gcaatccatg 300

ggacgcttca atactgacat ggtgcgaaga gtctattgag ctaattggta gtcctccggc 360

ccctgaatgc ggctaatcct aactgcggag cagatactca catgccagtg ggcagtctgt 420

cgtaacgggc aacttcgcag cggaaccgac tactttgggt gtccgtgttt ccttttattt 480

catactggct gcttatggtg acaattgaga gattgttacc atatagctat tggattggcc 540

atccagtgtc caacagagcg atcatttact tgtttgttgg attcgtaccc ttgaactaca 600

aagcactcgt aacgctcaaa cacattatag acctccacac agcaaa 646

<210> 141

<211> 555

<212> DNA

<213> Echovirus E30 (isolate 3-MRS2013)

<400> 141

actgagtatc aataagctgc tcacgtggct gaaggagaaa acgttcgtta tccgaccaat 60

tacttcgaga aacccagtac caccatgaaa gttgcgcggc gtttcgctcc gcacaacccc 120

agtgtagatc gggccgatga gtcaccgcgt tccccacggg cgaccgtggc ggtggctgcg 180

ttggcggcct gcccatggag caatccatgg gacgcttcaa tactgacatg gtgcgaagag 240

tctattgagc taattggtag tcctccggcc cctgaatgcg gctaatccta actgcggagc 300

agatacccac aagccagtgg gcagtctgtc gtaacgggca acttcgcagc ggaaccgact 360

actttgggtg tccgtgtttc cttttatttt atactggctg cttatggtga caattgagag 420

attgttacca tatagctatt ggattggcca tccggtgtcc aacagagcga tcatttactt 480

gtttgttgga ttcgtaccct tgaactacaa agcactcgta acgcttaaat acgttataga 540

cctcaacaca gcaaa 555

<210> 142

<211> 708

<212> DNA

<213> Echovirus E30 (isolate 4-MRS2013)

<400> 142

gcccactggg cgctagcaca ctggtatcac ggtacctttg tgcgcctgtt tttcctaccc 60

tttcccgaaa gaaacttaga agctcaactt gtgtggtcaa tagtaagctc agtacgccaa 120

ctgagccacg accaagcact tctgttaccc cggactgagt atcaataagc tgctcacgtg 180

gctgaaggag aaaacgttcg ttatccgacc aattacttcg agaaacccag taccaccatg 240

aaagttgcgc ggcgtttcgc tccgcacaac cccagtgtag atcgggccga tgagtcaccg 300

cgttccccac gggcgaccgt ggcggtggct gcgttggcgg cctgcccatg gagcaatcca 360

tgggacgctt caatactgac atggtgcgaa gagtctattg agctaattgg tagtcctccg 420

gcccctgaat gcggctaatc ctaactgcgg agcagatacc cacaagccag tgggcagtct 480

gtcgtaacgg gcaacttcgc agcggaaccg actactttgg gtgtccgtgt ttccttttat 540

tttatactgg ctgcttatgg tgacaattga gagattgtta ccatatagct attggattgg 600

ccatccggtg tccaacagag cgatcattta cttgtttgtt ggattcgtac ccttgtacta 660

caaaacactc gtaacgctta aatacgttat agacctcaac acagcaaa 708

<210> 143

<211> 741

<212> DNA

<213> Echovirus E30 (isolate 2012EM161)

<400> 143

ttaaaacagc ctgtgggttg ttcccacccg cagggcccac tgggcgctag cacactggta 60

ttacggtacc tttgtgcgcc tgttttattt cccttcccca aaacgtaatt tagaagcagc 120

acttcacgac caatagcagg cgtggcacac cagccatgtc ttggtcaagc acttctgtaa 180

ccccggactg agtatcaata aactgctcac gcggtcgaag gagaaaacgt ccgttacccg 240

gctaactact tcgagaaact tagtaacgcc atgaaaattg cgaagtgttt cgctcagcac 300

atccccagtg tagatcaggt tgatgagtca ccgcattccc cacaggtaac tgtggcggtg 360

gctgcgttgg cggcctgccc atggggtgac ccatgggacg cttcaatacg gacatggtgt 420

gaagagtcta ttgagctagt tagtagtcct ccggcccctg aatgcggcta atcctaactg 480

tggagcatgc gcccccactc cagagggtag cgtgtcgtaa tgggtaactc cgcagcggaa 540

ccgactactt tgggtgtccg tgtttcctct tattttatat tggctgctta tggtgacaat 600

tgaaagattg ttaccatata gctattggat tggccatccg gtgacaaaca gagctataat 660

ttaccagttt gtgggatttg ttccgttaag tttgaaagtc tttgatacat ttagtttaat 720

attattactt aatagtgcaa a 741

<210> 144

<211> 743

<212> DNA

<213> Echovirus E30 (isolate E30SD2010CHN)

<400> 144

ttaaaaccgc actgtgggtt ggacccaccc acagggccca atgggcgcta gcacactggt 60

atcacggtac ctttgtgcgc ctgttttatc cacccctccc cgaacgaaac ttagaagttt 120

gagttaaacg gtcaatagga gacccagtac accaactggg ccgtgaccaa gcacttctgt 180

gaccccggac cgagtatcaa taagctactc acgtggctga aggagaaacg ttcgttaccc 240

ggccaattac ttcgagaaac ttagtaccac catgaaggtt gcgcagcgtt tcgctccgca 300

caaccccagt gtagatcagg ccgatgagtc accgcgttcc ccacgggtga ccgtggcggt 360

ggctgcgttg gcggcctgcc cttggggcaa cccaagggac gcttcaatac tgacatggtg 420

cgaagagtct attgagctaa ttggtagtcc tccggcccct gaatgcggct aatcccaact 480

gcggagcaga tacccacata ccagtgggca gtctgtcgta atgggcaact ctgcagcgga 540

accgactact ttgggtgtcc gtgtttcctt tatttcttac actggctgct tatggtgaca 600

attgagagat tgttaccata tagctattgg attggccatc cggtgtctaa tagagcaatt 660

atctaccttt ttgttggatt tattccactt gactataaag cactcaaaac acttgaatac 720

attgtggatc ttaacacgac aaa 743

<210> 145

<211> 744

<212> DNA

<213> Echovirus E30 (isolate ECV30/GX10/05)

<400> 145

ttaaaacagc ctgtgggttg ctcccaccca cagggcccac tgggcgctag cacactggta 60

tcccggtacc tttgtgcgcc tgttttatcc atcccacccc cagctgtaac ttagaagttc 120

gacacacatg gtcaatagat ggctcagtgc accaactggg tttagaccaa gcacttctgt 180

taccccggac cgagtatcaa taagctgctc gcgcggctga aggagaaaac gttcgttacc 240

cggccaatta cttcgagaaa cctagtacca ccatggaggt tgcgcagcgt ttcgctccgc 300

acagccccag tgtagatcag gccgatgagc caccgcattc ctcacgggcg accgtggcgg 360

tggctgcgct ggcggcctgc ccatggggca acccatggga cgcttcaata ctaacatggt 420

gtgaagagtc tattgagcta attggtagtc ctccggcccc tgaatgcggc taatcctaac 480

tgcggagcag atactcacat gccagtgggc agtctgtcgt aacgggcaac tctgcagcgg 540

aaccgactac tttgggtgtc cgtgtttcct tatattctta tactggctgc ttatggtgac 600

aattgagaaa ttgttaccat atagctattg gattggccat ccagtgacaa acagagcaat 660

tgtttaccta tttgttggct tcataccgtt gaattttaga gttttgaaga cccttaactt 720

gattacacta tttaactctg taaa 744

<210> 146

<211> 744

<212> DNA

<213> Echovirus E30 (strain Kor08-ECV30 )

<400> 146

ttaaaacagc ctgtgggttg ctcccaccca cagggcccac tgggcgctag cacactggta 60

tcccggtacc tttgtgcgcc tgttttatcc accccacccc cagctgtaat ttagaagttc 120

gacacacatg gtcaatagat ggctcagtgc accaactggg tttagaccaa gcacttctgt 180

taccccgggc cgagtatcaa taagctgctc gcgcggctga aggagaaaac gctcgttatc 240

cggccaatta cttcgagaaa cctagtacca ccatggaggt tgcgcagcgt ttcgctccgc 300

acagccccag tgtagatcag gccgatgagt caccgcgttc ctcacgggcg accgtggcgg 360

tggctgcgtt ggcggcctgc ccatggggca acccatggga cgcttcaata ctaacatggt 420

gtgaagagtc tattgagcta attggtagtc ctccggcccc tgaatgcggc taatcctaac 480

tgcggagcag atactcacat gccagtgggc agtctgtcgt aacgggcaac tctgcagcgg 540

aaccgactac tttgggtgtc cgtgtttcct tttattctta tactggctgc ttatggtgac 600

aattgagaaa ttgttaccat atagctattg gattggccat ccagtgacaa acagagcgat 660

tgtttaccta tttgtcggtt tcataccgtt gaactttaga gttttgaaga ccctcaactt 720

gattatatta ttcaactctg taaa 744

<210> 147

<211> 743

<212> DNA

<213> Echovirus E30 (isolate FDJS03_84)

<400> 147

ttaaaacagc ctgtgggttg gacccaccca tagggcccac tgggcgctag cacactggta 60

tcacggtacc tttgtgcgcc tgttttatcc acccttcccc gaacgaaact tagaagtttg 120

acttaaacgg tcaatagaaa acccagtaca ccaactgggt cgtgaccaag cacttctgtg 180

accccggact gagtatcaat aagctactca cgtggctgaa ggaggaaacg ttcgttaccc 240

ggccaattac ttcgagaaac ttagtaccac catgaaggtt gcgtagcgtt tcgctccaca 300

cgaccccagt gtagatcagg tcgatgagtc accgcgttcc ccacgggtga ccgtggcggt 360

ggctgcgttg gcggcctgcc cttggggcaa cccaagggac gcttcaatac tgacatggtg 420

cgaagagtct attgagctaa ttggtagtcc tccggcccct gaatgcggct aatcccaact 480

gcggagcaga tacccacatg ccagtgggca gtctgtcgta acgggcaact ctgcagcgga 540

accgactact ttgggtgtcc gtgtttcctt ttattcttat actggctgct tatggtgaca 600

attgagagat tgttaccata tagctattgg attggccatc cagtgtctaa tagagcaatc 660

atctaccttt ttgttggatt tattccactt gactataaag cactcaaaac acttgaatac 720

attgtaaatc ttaacacaac aaa 743

<210> 148

<211> 750

<212> DNA

<213> Echovirus 30 (strain Bastianni)

<400> 148

ttaaaacagc ctgtgggttg atcccaccca cagggcccac tgggcgccag cacactggta 60

tcgtggtacc tttgtgcgcc tgttttatac cccctacccc cacatgtact ctagaagcaa 120

ggcaaacctg accaatagta ggtgtggcac accagccgca ttttggtcaa gcacttctgt 180

ttccccggac cgagtatcaa taagctgctc acgcggctga aggagaaacc gttcgttacc 240

cgaccagcta cttcgagaaa cctagtaaca ctatgaacgt tgcggagtgt ttcgttcagc 300

acttcccccg tgtagatcag gtcgatgagt caccgcattc ctcacgggtg accgtggcgg 360

tggctgcgtt ggcggcctgc ctacgggttc gcccgtagga cgctctaata ccgacatggt 420

gtgaagagtc cattgagcta gctggtagtc ctccggcccc tgaatgcggc taatcctaac 480

tgcggagcag gtgctcacag accagtgagt agcctgtcgt aacgggcaac tctgcagcgg 540

aaccgactac tttgggtgtt tttccttttt tcttctctta tattggctgc ttatggtgac 600

aattaaagaa ttgttaccat atagctattg gattggccat ccggtgacga gcagagccat 660

tgtttacctc tttgttggat ttgtaccttt gaaccacaaa gtcttgaata ccattcatct 720

cattttaaag ttcaactcag ctaaaagaaa 750

<210> 149

<211> 741

<212> DNA

<213> Echovirus 31 (strain Caldwell)

<400> 149

ttaaaacagc ctgtgggttg ttcccaccca cagggcccat gggcgctagc acactggtat 60

cgtggtacct ttgtgcgcct gttttataca ccctccccat ttgtaactta gaagtaaagc 120

aatattgatc aatagcaagc atggcacacc agccgtgttc agatcaagca cttctgtaac 180

cccggactga gtatcaatag gctgccaaag cggctgaagg agaaaacgtt cgttaaccgg 240

ccaactactt cgagaagccc agtatcacca tgaaagttgc agagcgtttc gctcagcact 300

ccccccgtgt agattaggtc gatgagtcac cgcatccccc acaggcgact gtggcggtgg 360

ctgcgttggc ggcctaccta cggggcaacc cgtaggacgc tctaatacag acatggtgag 420

aagagcctat tgagctagtt ggtagtcctc cggcccctga atgcggataa tcctaactgc 480

ggagcatgtg ctcgcaatcc agcgagaggc atgtcgtaac gggcaactct gcagcggaac 540

cgactacttt gggtgtccgt gtttcctttt attccttgtt tggctgctta tggtgacaat 600

tgaaagattg ttgccatata gctattgggt cggccacctg gtatctaaca gagcaattat 660

ttacttgttt gttggttttg tacccttaaa tctcaaggtt cttgaaaccc ttaactacac 720

attgaaactc aattcattga a 741

<210> 150

<211> 732

<212> DNA

<213> Echovirus 32 (strain PR-10)

<400> 150

tgtgggttgt tcccacccac agggcccact gggcgctagt acactggtac caaggtacct 60

ttgtacgcct gttttatacc ccctccccga tccgaaactt agaagtaaag caaacccgat 120

caatagtagg tgcgacactc cagtcgtatc tcgatcaagc acttctgttt ccccggactg 180

agtatcaata gactgcccac gcggttgaag gagaaaacgt ccgtcatccg gctaactact 240

tcgagaaacc cagtagcacc atggaagttg cagagtgttt cgctcagcac taaccccgtg 300

tagatcaggc tgatgagtca ccgcattccc cacgggtgac cgtggcggtg gctgcgttgg 360

cggcctgcct atggggcaac ccataggacg ctctaatacg gacatggtgt gaagagtcta 420

ttgagctagt tagtaatcct ccggcccctg aatgcggcta atcctaactg cggagcacat 480

actcctactc caaggagcag tgtgtcgtaa cgggcaactc tgcagcggaa ccgactactt 540

tgggtgtccg tgtttcctat taacatatca ctggctgctt atggtgacaa ttgaaagatt 600

gttaccatat agctattgga ttggccatcc ggtgtcaaac agagcaatag tgtacctatt 660

cgctgggttt atcccattga ctgttaaaga tatacaaact ctctacttga taatacaact 720

aaacactgta aa 732

<210> 151

<211> 743

<212> DNA

<213> Echovirus E33 (strain YNK35/CHN/2013)

<400> 151

ttaaaacagc ctgtgggttg ttcccaccca cagggcccac gtggcgttag cacactggta 60

tcccggtacc tttgtgcgcc tgttttatac accccaaacc ccaatgtaaa ttagaagaaa 120

ataacaccga tcaatagtgg gtgtggcaca acagccacat catgatcaag cactactgtt 180

cccccggact gagtatcaat aagctgctcg cgcggctgaa ggagaaaacg ttcgttatcc 240

ggccaattac ttcgagaaac ctagtaccac catgaaggtt gcgcagtgtt tcgctccaca 300

caaccccagt gtagatcagg tcgatgagtc accgcattcc ccacaggcga ctgtggcggt 360

ggctgcgctg gcggcctgcc catggggtga cccatgggac gcttcaatac tgacatggtg 420

tgaagagtct attgagctaa ttggtagtcc tccggcccct gaatgcggct aatcctaact 480

gcggagcaga tacccacgca ccagtgggta gtctgtcgta acgggtaact ctgcagtgga 540

accgactact ttgggtgtcc gtgtttcctt ttattctcac attggctgct tatggtgaca 600

attgaaagat tgttaccata tagctactgg attggccatc cggtgacaaa cagagcaatt 660

gtgttcctct tcgatgggtt catacctctc aacaagatca gctttcgaac tttgtgctac 720

atcatcaagc taaacacaac aaa 743

<210> 152

<211> 743

<212> DNA

<213> Echovirus E33 (strain YNA12/CHN/2013)

<400> 152

ttaaaacagc ctgtgggttg ttcccaccca cagggcccac gtggcgttag cacactggta 60

tcccggtacc tttgtgcgcc tgttttatac accccaaacc ccaatgtaac ttagaagaaa 120

ataacaccga tcaatagtgg gtgtggcaca ccagccacat catgatcaag cactactgtt 180

cccccggact gagtatcaat aagctgctcg cgcggctgaa ggagaaaacg ttcgttatcc 240

ggccaattac ttcgagaaac ctagtaccac catgaaggtt gcgcagtgtt ttgctccaca 300

caaccccagt gtagatcagg tcgatgagtc accgcattcc ccacaggcga ctgtggcggt 360

ggctgcgctg gcggcctgcc catggggtga cccatgggac gcttcaatac tgacatggtg 420

tgaagagtct attgagctaa ttggtagtcc tccggcccct gaatgcggct aatcctaact 480

gcggagcaga tacccacgca ccagtgggca gtctgtcgta acgggtaact ctgcagcgga 540

accgactact ttgggtgtcc gtgtttcctt ttattctcac attggctgct tatggtgaca 600

attgaaagat tgttaccata tagctactgg attggccatc cggtgacaaa cagagcaatt 660

gtgttcctct tcgttgggtt catacctctc aacaagatca gctttcgaac tttgtgctac 720

atcatcaagc taaacacaac aaa 743

<210> 153

<211> 747

<212> DNA

<213> Human poliovirus 1 (isolate CHN-Hainan/93-2)

<400> 153

ttaaaacagc ctgtgggttg tacccaccca cagggcccac tgggcgctag cactctgatt 60

ccacggaatc tttgtgcgcc tgttttataa cccttccccc catttcgcaa cttagaagca 120

acgcgcatca ctgatcaaca gcaggcatgg cgttccagcc atgtcttgat caagcactcc 180

tgtttccccg gactgagtat caatagactg cttacgcggt tgaaggagaa aacgttcgtt 240

acccggctaa ctacttcgag aaacttagta acgccatcga agctgcaggg tgtttcgctc 300

agcacttccc ccgtgtagat caggccgatg agtcaccgca atccccacgg gcgaccgtgg 360

cggtggctgc gttggcggcc tgcctatggg gcaacccata ggacgcccta aagtggacat 420

ggtgcgaaga gtctattgag ctagttagta gtcctccggc ccctgaatgc ggctaatcct 480

aactgcggag cacataccct caagccagag ggcagtgtgt cgtaacgggt aactctgcag 540

cggaaccgac tactttgggt gtccgtgttt ccttttattc ttactatggc tgcttatggt 600

gacaattcaa gaattgttac catatagcta ttggattggc catccggtgt ctaacagagc 660

tattgtgtac ttatttgttg ggtacgttcc attatcttac acatccctta acacattacg 720

ttttatcgtg aagctaaata caagaag 747

<210> 154

<211> 745

<212> DNA

<213> Human poliovirus 1 (isolate RUS39223)

<400> 154

ttaaaacagc tctggggttg ttcccacccc agaggcccac gtggcggcta gcactctggt 60

atcacggtac ctttgtgcgc ctgttttatg tccccgcccc catgtaactt tagaagcaat 120

acacggtgtt cattaggagg ggcacaagcc agtgttccta tgaacaagca cttctgtttc 180

cccggtgacc tcgtataggc tgtgcccacg gctaaaaacg tgcgatccgt tacccgcttg 240

ggtacttcga gaagcccagt accaccttgg aatcttcgac gcgttgcgct cagcactcta 300

ccccgagtgt agcttaggct gatgagtctg ggcattcccc actggtgaca gtggcccagg 360

ctgcgttggc ggcctaccca ttgctcgcgc aatgggacgc tagatgtgaa caaggtgtga 420

agagcctatt gagctgccta agagtcctcc ggcccctgaa tgcggctaat cctaaccacg 480

gagcaagcgc cttcaaccca gggggtggct tgtcgtaacg cgcaagtctg tggcggaacc 540

gactactttg ggtgtccgtg tttccctttg tttttattat ggctgcttat ggtgacaatc 600

attgattgcc atcataaagc gaattggatt ggccatccgg tggaagttac atacatcatc 660

catctatttg ttggatttgc ttcactaaca cagttcacaa tcaacttggt atatataata 720

atagtgatta gagaaaaata ccata 745

<210> 155

<211> 743

<212> DNA

<213> Human poliovirus 1 (isolate Pak-1)

<400> 155

taaaacagct ctggggttgt ccccacccca gaggcccacg tggcggctag tactctggta 60

tcgcggtacc tttgtacgcc tgttttacac ccctacccca tgcaacttta gaagcaaatc 120

aataagttca ataggggggg gtacaaacca gtaccaccac gaacaagcac ttctgtaacc 180

ccggtgatct tgtataagct gtgcccacgg ctgaaagcga gtgatccgtt atccgcttag 240

gtacttcgag aagcctagta tcaccttgga atcttcgatg cgttgcgctc agcactctat 300

cccgagtgta gcttaggctg atgagtctgg gcgttcccca ccggcgacgg tggcccaggc 360

tgcgttggcg gcctacccat ggctaacgcc atgggacgct agatgtgaac aaggtgtgaa 420

gagcctattg agctacttaa gagtcctccg gcccctgaat gcggctaatc ccaaccacgg 480

agcaggtgcc ttcaacccag agggtggcct gtcgtaacgc gcaagtctgt ggcggaaccg 540

actactttgg gtgtccgtgt ttccttttat cttttatatg gctgcttatg gtgacaatca 600

tagattgtta tcataaagtg acttggattg gccatccggt aaaatacaaa cacatcatct 660

acttgtttgt tggatttacc ccactcaccc aatttactcc cagcataaca tccattgttt 720

gttaacaaga tactgctacc aca 743

<210> 156

<211> 745

<212> DNA

<213> Human poliovirus 1 (isolate TJK35363 clone 6)

<400> 156

ttaaaacagc tctggggttg ttcccacccc agaggcccac gtggcggcta gcactctggt 60

atcacggtac ctttgtgcgc ctgttttatg tccccgcccc catgtaactt tagaagcaat 120

acacggtgtt cattaggagg ggcacaagcc agtgttccta tgaacaagca cttctgttcc 180

cccggtgacc tcgtataggc tgtgcccacg gctaaaaacg agcgatccgt tacccgcttg 240

ggtacttcga gaagcctagt accaccttgg aatcttcgac gcgttgcgct cagcactcta 300

ccccgagtgt agcttaggct gatgagtctg ggcattcccc actggtgaca gtggcccagg 360

ctgcgttggc ggcctaccca tggctcgcgc catgggacgc tagatgtgaa caaggtgtga 420

agagcctatt gagctaccta agagtcctcc ggcccctgaa tgcggccaat cctaaccacg 480

gagcaagcgc cttcaaccca gggggtggct tgtcgtaacg cgcaagtctg tggcggaacc 540

gactactttg ggtgtccgtg tttcccttta tttttactat ggctgcttat ggtgacaatc 600

attgattgcc atcataaagc gaattggatt ggccatccgg tggaagttat atacatcatc 660

tatctatttg ttggatttgc ttcactaaca cagttcacaa tcaacttggt atatataata 720

atagtgatta gagaaaaata ccata 745

<210> 157

<211> 554

<212> DNA

<213> Human poliovirus 1 (strain 3788ALB96)

<400> 157

gtataggctg tactcacggc tgaaaacaaa cgatccgtta tccgcttagg tacttcgaga 60

agcctagtac catcttggaa tcttcgacgc gttgcgctca gcactctacc ccgagtgtag 120

cttaggctga tgagtctggg cgttccccac cggtgacggt ggcccaggct gcgttggcgg 180

cctacccatg gctcacgcca tgggacgcta gttgtgaaca aggtgtgaag agcctattga 240

gctacctaag agtcctccgg cccctgaatg cggctaatcc taaccacgga gcaagtgcct 300

tcagcccaga aggtagcttg tcgtaacgcg caagtctgtg gcggaaccga ctactttggg 360

tgtcccgtgt ttccccttat tttcattgtg gctgcttgcg ggactataat tgattgccat 420

cataaagcga tttggattgg ccatccggag aaagttaaac acgttgttta tttattcgtt 480

ggattcacct cattaaccca aattacaccc agcttgatat atatagtgtt gccgactaga 540

caaaatcacc acca 554

<210> 158

<211> 744

<212> DNA

<213> Human poliovirus 1 (isolate CHN15115/Xinjiang/CHN/2011)

<400> 158

tttaaacagc tctggggttg ttcccacccc agaggcccac gtggcggcta gtactctggt 60

atcgcggtac ctttgtacgc ctgttttata cccctacccc atgtaacttt agaagcagtt 120

caacaagttc aatagggggg gtgcaaacca gcaccaccac gaacaagcac ttctgtaacc 180

ccggtgacct tgtataagct gtgcccacgg ctgaaagcga gtgatccgtt atccgcttaa 240

gtacttcgag aagcttagta tcaccttgga atcttcgatg cgttgcgctc agcactctat 300

cccgagtgta gcttaggctg atgagtctgg gcgttcccca ccggcgacgg tggcccaggc 360

tgcgttggcg gcctacccat ggctaacgcc atgggacgct agatgtgaac aaggtgtgaa 420

gagcctattg agctacttaa gagtcctccg gcccctgaat gcggctaatc ccaaccacgg 480

agcaggtgcc ttcaacccag agggcggcct gtcgtaacgc gcaagtctgt ggcggaaccg 540

actactttgg gtgtccgtgt ttccttttat cttttatatg gctgcttatg gtgacaatca 600

tagattgtta tcataaagcg acttggattg gccatccggt aaaatacaaa cacatcatct 660

acttgtttgt tggatttact ccactcaccc aatttactcc cagcataata tccattgttt 720

tgttaacaag acattgctat cacg 744

<210> 159

<211> 737

<212> DNA

<213> Human poliovirus 1 (isolate 29690_c1)

<400> 159

acagctctgg ggttgcaccc accccagagg cccacgtggc ggctagtact ccggtattgc 60

ggtacccttg tacgcctgtt ttatactccc ttcccgtaac ttagacgcac aaaaccaagt 120

tcaatagaag ggggtacaaa ccagtaccac cacgaacaag cacttctgtt tccccggtga 180

tgttgtatag actgcttgcg tggttgaaag cgacggatcc gttatccgct tatgtacttc 240

gagaagccca gtaccacctc ggaatcttcg atgcgttgcg ctcagcactc aaccccagag 300

tgtagcttag gctgatgagt ctggacatcc ctcaccggtg acggtggtcc aggctgcgtt 360

ggcggcctac ctatggctaa cgccatggga cgctagttgt gaacaaggtg tgaagagcct 420

attgagctac ataagaatcc tccggcccct gaatgcggct aatcccaacc tcggagcagg 480

tggtcacaaa ccagtgattg gcctgtcgta acgcgcaagt ccgtggcgga accgactact 540

ttgggtgtcc gtgtttcctt ttattttatt gtggctgctt atggtgacaa tcacagattg 600

ttatcataaa gcgaattgga ttggccatcc ggtgaaagtg agatttatta tctatctgtt 660

tgctggattc gctccattaa gtgtgtttac tctaagtata atttcaacag ttatttcaat 720

cagacaattg tatcata 737

<210> 160

<211> 713

<212> DNA

<213> Human poliovirus 1 (strain NIE1018316)

<400> 160

cagaggccca cgtggcggct agtactccgg tattgcggta cccttgtacg cctgttttat 60

actcccttcc cgtaacttag acgcacaaaa ccaagttcaa tagaaggggg tacaaaccag 120

taccaccacg aacaagcact tctgtttccc cggtgatgtt gtatagactg cttgcgtggt 180

tgaaagcgac ggatccgtta tccgcttatg tacttcgaga agcccagtac cacctcggaa 240

tcttcgatgc gttgcgctca gcactcaacc ccagagtgta gcttaggctg atgagtctgg 300

acatccctca ccggtgacgg tggtccaggc tgcgttggcg gcctacctat ggctaacgcc 360

atgggacgct agttgtgaac aaggtgtgaa gagcctattg agctacataa gaatcctccg 420

gcccctgaat gcggctaatc ccaacctcgg ggcaggtggt cacaaaccag tgattggcct 480

gtcgtaacgc gcaagtccgt ggcggaaccg actactttgg gtgtccgtgt ttccttttat 540

tttaatgtgg ctgcttatgg tgacaatcac agattgttat cataaagcga attggattgg 600

ccatccggtg aaagtgagat tcattatcta tctgtttgct ggattcgctc cattgagtgt 660

gtttactcta agtacaattt caacagttat ttcaatcaga caattgtatc ata 713

<210> 161

<211> 744

<212> DNA

<213> Human poliovirus 1 (isolate EGY1218587)

<400> 161

ttaaaacagc tctggggttg ttcccacccc agaggcccac gtggcggcta gtactctggt 60

atcgcggtac ctttgtacgc ctgttttata cccccacccc atgtaacttt agaagcagtt 120

caacaagttc aatagggggg gtgcaaacca gcaccaccac gaacaagcac ttctgtaacc 180

ccggtaacct tgtataagct gtacccacgg ctgaaagcga gtgatccgtt atccgcttaa 240

gtacttcgag aagcctagta tcaccttaga atcttcgatg cgttgcgctc agcactctat 300

cccgagtgta gcttaggctg atgagtctgg gcgttcctca ccggcgacgg tggcccaggc 360

tgcgttggcg gcctacccat ggctaacgcc atgggacgct agatgtgrac aaggtgtgaa 420

gagcctattg agctacttaa gagtcctccg gcccctgaat gcggctaatc ccaaccacgg 480

agcaggtgcc ttcaacccag agggtggcct gtcgtaacgc gcaagtctgt ggcggaaccg 540

actactttgg gtgtccgtgt ttccttttat cttttatatg gctgcttatg gtgacaatca 600

tagattgtta tcataaagcg acttggattg gccatccggt aaaatacaaa cacatcattt 660

acttgtttgt tggatttact ccactctccc aatttactcc tagcataata tccattgttt 720

tgttaacaag acattactat caca 744

<210> 162

<211> 747

<212> DNA

<213> Human poliovirus 1 (isolate 558/BRA-PE/88)

<400> 162

ttaaaacagc tctggggttg ttcccacccc agaggcccac gtggtggcca gtactctggt 60

attacggtac ctttgtacgc ctgttttata ctcccttccc ctgtaactta gaagcaatct 120

aacctagttc aataggaggg ggtacaaacc agtaccacta cgaacaagca cttctgttac 180

cccggtgaag ttgtatagac tgtgcccacg gtcgaaaatg actgatccgt tatccgctca 240

tgtacttcgg gaagcctagt accatcttcg gatcttcgat gcgttgcgct cagcactcga 300

ccccagagtg tagcttaggt cgatgagtct ggacaatcct cactggcgac agtggtccag 360

gctgcgttgg cggcctacct gtggtccaaa gccacaggac gctagttgtg aacaaggtgt 420

gaagagccta ctgagctact tgagagtcct ccggcccctg aatgcggcta atcccaacca 480

cggagcaagt agttgcaaac cagcaaccag cttgtcgtaa cgcgtaagtc tgtggcggaa 540

ccgactactt tgggtgtccg tgtttccttt tatttttaca ctggctgctt atggtgacaa 600

tcatagattg ttatcataag gcgaattgga ttggccaccc ggtgtaagtc agatcaattg 660

tctactcgtt tgttggatat actccaataa cgcactacac tcttgagctt aacagagttg 720

ttctgctggc gagaagttgc tatcgca 747

<210> 163

<211> 744

<212> DNA

<213> Human poliovirus 2 (isolate Env2008_E2450)

<400> 163

ttaaaaccag ctgtgggttg ttcccacccc agaggcccac gcggcggcta gcactccggt 60

attacggtac ccttgtgcgc ctgttttata ctcccttccc ccgcaattta gaagcatgaa 120

accaggttca atagtaaggg gtacaaacca gtaccactat gaacaagcac ttctgtctcc 180

ccggtgatat tgcatagact gctcacgcgg ttgaaagcga ttgatccgtc atccgcttgt 240

gtacttcgag aagcctagta tcaccttcgg aacttcgacg cgttgcgctc agcacccgac 300

cccggggtgt agcttgggct gatgagtctg gacgctcctc accggcgacg gtggtccagg 360

ctgcgttggc ggcctacccg tggttaccac cgcgggacgc tacaagtgaa caaggtgtga 420

agagcctatt gagctacaca agagtcctcc ggcccctgaa tgcggctaat cccaaccacg 480

gagcaggcgg tcacgaacag tggctggctt gtcgtaacgc gcaagtctgt ggcggaaccg 540

actactttgg gtgtccgtgt ttcctgttat ttttatcatg gctgcttatg gtgacaatcg 600

ttgattgtta tcataaagcg aattggattg gccgtccgga aagtgttgtg tcgcatatac 660

tgtttagtga agctactaca ttaattcaat ctctcactcg gttagtcaaa cagagctcag 720

atagagtaaa acatcattaa cgcg 744

<210> 164

<211> 747

<212> DNA

<213> Human poliovirus 2 (strain CHA1218985)

<400> 164

ttaaaacagc tctggggttg ttcccacccc agaggcccac gtggcggcca gtactccggt 60

attacggtac ccttgtacgc ctgttttata ctcccttccc ctgtaactta gaagcataca 120

aaccaagttc aatagaaggg ggtacaaacc agtaccacca cgaacaagca ctcctgtttc 180

cccggtgaca ttgcatagac tgtacccacg gctgaaagcg atcgatccgt tacccgctcc 240

tgtacttcga gaagcctagt atcatcttgg aatcttcgat gcgttgcgct cagcactcaa 300

ccccagagtg tagcttaggc tgatgagtct ggacgtcccc caccggcgac ggtggtccag 360

gctgcgttgg cggcctacct gtggcccaaa gccacaggac gctagttgtg aacaaggtgt 420

gaagagccta ttgagctaca agagagtcct ccggcccctg aatgcggcta atcctaacca 480

cggagcaggc agttgcaaac cagcaaccgg cctgtcgtaa cgcgcaagtc tgtggcggaa 540

ccgactactt tgggtgtccg tgtttccttt tatttttaca atggctgctt atggtgacaa 600

tcatagattg ttatcataaa gcgacttgga ttggccatcc ggtgaaagta aaacacattg 660

tttacttgtt tgttggattc actccaatta actcttttac ttacaaactc attacaacaa 720

ctctattaat tagagataag catcaca 747

<210> 165

<211> 745

<212> DNA

<213> Human poliovirus 2 (isolate Env2008_E3218)

<400> 165

ttaaaacagc tctggggttg ttcccacccc agaggcccac gtggcggcta gcactccggt 60

attacggtac ccttgtgcgc ctgttttata ctcccttccc ccgcaattta gaagcatgaa 120

acccggttca atagtaaggg gtacaaacca gtaccactat gaacaagcac ttctgtctcc 180

ccggtgatat tgtatagact gctcacgcgg ttgaaagcga ttgatccgtt atccgcttgt 240

gtacttcgag aagcctagta ccaccttcgg aacttcgacg cgttgcgctc agcacccgac 300

cccggggtgt agcttgggct gatgagtctg gacgatcctc accggcgacg gtggtccagg 360

ctgcgttggc ggcctacccg tggttcacac cacgggacgc tacaagtgaa caaggtgtga 420

agagcctatt gagctacaca agagtcctcc ggcccctgaa tgcggctaat cccaaccacg 480

gagcaggcgg tcacgaacca gtggctagct tgtcgtaacg cgcaagtctg tggcggaacc 540

gactactttg ggtgtccgtg tttcctgtta tttttatcat ggctgcttat ggtgacaatc 600

attgattgtt atcataaagc gaattggatt ggccgtccgg aaagtgtttt atcacatata 660

ctgtttggtg aagctactac attaattctg cctctcactc ggttagtcaa acagaactca 720

gacagagtaa aacatcacca acgca 745

<210> 166

<211> 712

<212> DNA

<213> Human poliovirus 2 (strain MAD-2593-11)

<400> 166

cccacgtggc ggccagtact ccggtatcac ggtacccttg tacgcctgtt ttatactccc 60

ttcccccgta acttagaagc aatgaaacca agttcaatag aagggggtac aaaccagtac 120

caccacgaac aagcacttct gtttccccgg tgacgttgca tagactgtcc ccacggttga 180

aggcggctga tccgttatcc gcttgtgtac ttcgagaagc ctagtaccat cttggaatct 240

ccgatgcgtt gcgctcagca ctcaacccca gagtgtagct taggctgatg agtctggaca 300

ttccccaccg gcgacggtgg tccaggctgc gttggcggcc tacctgtggt ccaaaaccac 360

aggacgctag ttgtgaacaa ggtgtgaaga gcctattgag ctacatgaga gtcctccggc 420

ccctgaatgc ggctaatccc aaccacggag caggtgtttg caatccagca aatagcctgt 480

cgtaacgcgc aagtctgtgg cggaaccgac tactttgggt gtccgtgttt ccttttattt 540

tcatactggc tgcttatggt gacaatctcg gattgttatc atagagcaaa ttggattggc 600

catccggtga aaatcagatt gattatttaa ctttttgttg gatttacatc tttgacatca 660

catactccga atctcattaa gatagtgctc ttgatacgac atcatcgtca tt 712

<210> 167

<211> 748

<212> DNA

<213> Human poliovirus 3 (strain PAK1019536)

<400> 167

tggggttaaa acagctctgg ggttgttccc accccagagg cccacgtggc ggctagtact 60

ctggtatcat ggtacctttg tacgcctgtt ttatacccct accccacgta actttagaag 120

caactcaaca agttcaatag ggggggtaca aaccagtacc accacgaaca agcactcctg 180

ttaccccggt gatcttgcat aagctgtgcc cacggctgaa ggcgagagat ccgttatccg 240

cttgagtact tcgagaagcc tagtatcacc ttgggatctt cgacgcgttg cgctcagcac 300

tccacccgag tgtagcttag gctgatgagc ctgggcattc cccaccggcg acggtggccc 360

aggctgcgtt ggcggcctac ccatggctaa caccatggga cgctagatgt gaacaaggtg 420

tgaagagcct attgagctac tcaagagtcc tccggcccct gaatgcggct aatcccaacc 480

acggagcagg tgtcttcaac ccagaaggta gcctgtcgta acgcgtaagt ctgtggcgga 540

accgactact ttgggtgtcc gtgtttcctt ttatctttta tatggctgct tatggtgaca 600

atcacagatt gttatcataa agcgatttgg attggccatc cggtgaagta caaacacatt 660

atctacttgt ttgttggatt tactccactc acacacccca cccccaatat aacatctatt 720

gttttgttaa caagaaatta ctttcgaa 748

<210> 168

<211> 729

<212> DNA

<213> Human poliovirus 3 (isolate Env08_E2886)

<400> 168

ttaaaaacag ctctggggtt gttcccaccc cagaggccca cgtggcggct agtactctgg 60

tattacggta cctttgtacg cctgttttat atcccctttc ccctgtaact cagaagcatg 120

aagtttaagc tcaacaggag gggtgcaaac cagcacctct acgagcaagc actactgttt 180

ccccggtgaa gttacataga ctgttcccac ggttgaaagt gattgatccg ttatccgctc 240

atgtacttcg agaagcctag tatcgctctg gaatcttcga tgcgttgcgc tcaacactcg 300

accccggagt gtagcttagg ccgatgagtc tggacattcc ccactggcga cagtggtcca 360

ggctgcgttg gcggcctacc tgtggtccaa agccacagga cgctagttgt gaacagggtg 420

tgaagagcct attgagctgc atgagagtcc tccggcccct gaatgcggat aatcctaacc 480

atggagcagg caactgcaac ccagcagtca gcctgtcgta acgcgtaagt ccgtggcgga 540

accgactact ttgggtgtcc gtgtttcctt ttatttttga atggctgctt atggtgacaa 600

tcacagattg ttatcataaa gcaaattgga ttggccatcc agtgaatcac taccgagttt 660

gttaaatcta tttcagagac acacaactcc tcaacgcact atacctaaac agaggattat 720

actacagcg 729

<210> 169

<211> 746

<212> DNA

<213> Human poliovirus 3 (strain SWI10947)

<400> 169

ttaaaacagc tctggggttg ttcccacccc agaggcccac gtggcggcta gtactccggt 60

atcacggtac ccttgtacgc ctgttttata ctcccttccc cgtaacttag aagtatacaa 120

accaagttca atagaagggg gtacaaacca gtaccaccac gaacaagcac ttctgtttcc 180

ccggtgaagt tgcatagatt gtccccacgg tcgaaagcga ctgatccgtt atccgctctt 240

gtacttcggg aagcctagta tcaccttcgg atcttcgatg cgttgcgctc agcactcaac 300

cccagagtgt agcttaggtc gatgagtctg gacgaacctc actggtgaca gtggtccagg 360

ctgcgttggc ggcctacctg tggcccaaag ccacaggacg ctagttgtga acaaggtgtg 420

aagagcctac tgagctacat gagagtcctc cggcccctga atgcggctaa tcccaaccac 480

ggagcaggtg gtcacaaacc agtggtttgc ctgtcgtaac gcgaaagtct gtggcggaac 540

cgactacttt gggtgtccgt gtttcctttt attcttatta tggctgctta tggtgacaat 600

cttagattgt tatcataaag cgaattggat tggccatccg gggaaaatta agcatatcat 660

acacttgttt gttggattca cccctctgat tacacatact cttggcttga ttaggattgt 720

tgcattgata agacactatt gttgca 746

<210> 170

<211> 732

<212> DNA

<213> Human poliovirus 3 (strain FIN84-2493)

<400> 170

gggttgttcc caccccagag gcctacgtgg cggctagtac tccggtatca cggtaccctt 60

gtacgcctgt tttatactcc cttccccgta acttagaagt aaacaagcca agttcaatag 120

aagggggtac aaaccagtac caccacgaac aagcacttct gtttccccgg tgaagttgca 180

tagattgttc ccacggtcga aagcgactga tccgttaccc gctcatgtac ttcgggaagc 240

ctagtatcac cttcggatct tcgatgcgtt gcgctcagca ctcaacccca gagtgtagct 300

taggtcgatg agtctggaca aacctcactg gcgacagtgg tccaggctgc gttggcggcc 360

tacctgtggc ccaaagtcac aggacgctag ttgtgaacaa ggtgtgaaga gcctactgag 420

ctacatgaga gtcctccggc ccctgaatgc ggctaatctc aaccacggag caggtagtca 480

caaaccagtg acttgcctgt cgtaacgcga aagtctgtgg cggaaccgac tactttgggt 540

gtccgtgttt ccttttattc tcattatggc tgcttatggt gacaatccta gattgttatc 600

ataaagcgag ttggattggc catccggtga aaatcaagca catcatatac ttgtttgttg 660

ggtataaccc tctgattgca cgcactcttg gcttggttag gattattgtg tggaaaaggc 720

accatagtta ca 732

<210> 171

<211> 747

<212> DNA

<213> Human poliovirus 3 (strain USOL-D-bac)

<400> 171

ttaaaacagc tctggggttg ctcccacccc agaggcccac gtggcggcca gtactctggt 60

attgcggtac ctttgtacgc ctgttttata ctccctcccc ccgtgcaact ttagaagaaa 120

tccacaaagt tcaatagagg gggtgcaaac cagcaccacc acgaacaagc acttctgttt 180

ccccggtgat gtcgtataag ctgtacccac ggctgaaggc gacggatccg ttatccgctt 240

gagtacttcg agaagcctag tattaccttg gaatcttcga cgcgttgcgc tcagcactct 300

accccgagtg tagcttaggt cgatgagtct gggcacgccc caccggcgac ggtggcccag 360

gctgcgttgg cggcctaccc atggctatca ccatgggacg ctagttgtga acaaggtgtg 420

aagagcctat tgagctaccc aagagtcctc cggcccctga atgcggctaa tcctaaccac 480

ggagcaagtg tcctcaaccc aggggatggc ttgtcgtaac gcgaaagtct gtggcggaac 540

cgactacttt gggtgtccgt gtttcctttt atttttatgt atggctgctt atggtgacaa 600

tcaaaggttg ttaccataaa gcaatttgga ttggccatcc ggtgagaatc aaacatatta 660

tctacctgtt tgttgggttt tcttctttta cttgaacaat acctctaata ataactgcta 720

tattgtcaat aagacattat catcaca 747

<210> 172

<211> 747

<212> DNA

<213> Enterovirus A71 (isolate 2019-EV-A71-R398)

<400> 172

ttaaaacagc ctgtgggttg tacccaccca cagggcccac tgggcgctag cactctgatt 60

ccacggaatc tttgtgcgcc tgttttataa cccttccccc catttcgcaa cttagaagca 120

acgcgcatca ctgatcaaca gcaggcatgg cgttccagcc atgtcttgat caagcactcc 180

tgtttccccg gactgagtat caatagactg cttacgcggt tgaaggagaa aacgttcgtt 240

acccggctaa ctacttcgag aaacttagta acgccatcga agctgcaggg tgtttcgctc 300

agcacttccc ccgtgtagat caggccgatg agtcaccgca atccccacgg gcgaccgtgg 360

cggtggctgc gttggcggcc tgcctatggg gcaacccata ggacgcccta aagtggacat 420

ggtgcgaaga gtctattgag ctagttagta gtcctccggc ccctgaatgc ggctaatcct 480

aactgcggag cacataccct caagccagag ggcagtgtgt cgtaacgggt aactctgcag 540

cggaaccgac tactttgggt gtccgtgttt ccttttattc ttactatggc tgcttatggt 600

gacaattcaa gaattgttac catatagcta ttggattggc catccggtgt ctaacagagc 660

tattgtgtac ttatttgttg ggtacgttcc attatcttac acatccctta acacattacg 720

ttttatcgtg aagctaaata caagaag 747

<210> 173

<211> 747

<212> DNA

<213> Enterovirus A71 (strain USA/2018-23296)

<400> 173

ttaaaacagc ctgtgggttg tacccaccca cagggcccac cgggcgctag cactctgatt 60

ccacggaatc tttgtgcgcc tgttttataa cccccctccc cacctcgcaa cttagaagca 120

acgcgcatca ctgatcaaca gcaggcatgg cgcgccagcc atgtcttgat caagcacttc 180

tgtttccccg gactgagtat caatagactg cttacgcggt tgaaggagaa aacgttcgtt 240

acccggctaa ctacttcgag aaacttagta acaccatcga agctgcaggg tgtttcgctc 300

agcacttccc ccgtgtagat caggccgatg agtcaccgca atccccacgg gcgaccgtgg 360

cggtggctgc gttggcggcc tgcctatggg gcaacccata ggacgcccta aagtggacat 420

ggtgcgaaga gtctattgag ctagttagta gtcctccggc ccctgaatgc ggctaatcct 480

aactgcggag cacataccct caagccagag ggtggtgtgt cgtaacgggt aactctgcag 540

cggaaccgac tactttgggt gtccgtgttt ccttttattc ttactgtggc tgcttatggt 600

gacaattcaa gagttgttac catatagcta ttggattggc catccggtgt ctaacagagc 660

tattgtgtac ttatttgttg ggtacgttcc attatcttac acatcccttg acacattaca 720

ttttattgtg aagctaaata caagaag 747

<210> 174

<211> 724

<212> DNA

<213> Enterovirus A71 (strain 16L)

<400> 174

ccgcccacag ggcccactgg gcgctagcac tctgattcca cggaatcttt gtgcgcctgt 60

tttatamyyy tctcccctat ttcgcaactt agaagcaacg cgcatcactg atcaacagca 120

ggcatggcgc tccagccatg tcttgatcaa gcactcctgt ttccccggac cgagtatcaa 180

tagactgctt acgcggttga aggagaaaac gttcgttayc cggctaacta cttcgagaaa 240

cttagtaaca ccatcgaagc tgcagggtgt ttcgctcagc acttcccccg tgtagatcag 300

gccgatgagt caccgcaatc cccacgggcg accgtggcgg tggctgcgtt ggcggcctgc 360

ctatggggca acccatagga cgccctaaag tggacatggy gcgaagagtc tattgagcta 420

gttagtagtc ctccggcccc tgaatgcggc taatcctaac tgcggagmwc rkamsctcaa 480

gccagagggt agtgtgtcgt aacgggcaac tctgcagcgg aaccgactac tttgggtgtc 540

cgtgtttcct tttattctta ctgtggctgc ttatggtgac aattcaagaa ttgttaccat 600

atagctattg gattggccat ccggtgtcta acagagctat tgtgtactta tttgttgggt 660

acgttccatt atcttacaca tcccttaaca cattacattt tattgtgaag ctaaatacaa 720

gaag 724

<210> 175

<211> 756

<212> DNA

<213> Enterovirus A76 (strain 10-3291-2)

<400> 175

cagacgtgtg ctcttcccga tgtgggttgt acccacccac agggcccaat gggcgctagc 60

acaccggtat cacggtaccc ttgtgcgcct gttttatgca ccctccccct atgtaactta 120

gaagaaacaa acacacgatc aatagtaggc ttggcgcgcc agctaagtct tgatcaagca 180

cttctgttac cccggactga gtatcaatag gctgtaccca cggctgaaag agaaaacgtt 240

cgttacccgg ctatctactt cgggaaacct agtaccatca tgaagactgc aaagcgtttc 300

gctcagcaca accccagtgt agatcaggtc gatgagtcac tgcattcctc acaggcgact 360

gtggcagtgg ctgcgttggc ggcctgccta tggggaaacc cataggacgc tttaatgttg 420

acatggtgcg aagagtctac tgagctaggt agtagtcctc cggcccctga atgcggctaa 480

tcccaaccac ggagcaagcg ctcacaatcc agtgagtagc ttgtcgtaat gcgaaagtct 540

gtggcggaac cgactacttt gggtgtccgt gtttcctttt atttttatca tggctgctta 600

tggtgacaat ctaagattgt tatcatatag ctattggatt ggccatccgg tgaactctag 660

agatattatc tatttgtttg ttggttttac caagttaaac atcactagtt acaccacctt 720

actttatatt atactactga ataatagaaa aagaaa 756

<210> 176

<211> 747

<212> DNA

<213> Human enterovirus A76 (AY697458)

<400> 176

tttaaaacag cctgtgggtt gtacccaccc acagggccca ctgggcgcta gcacaccggt 60

attacggtac ccttgtgcgc ctgttttata taccctcccc cttgtaactt agaagaaaca 120

aataaacgat caatagtagg cttagcgcac cagctaagtc ttgatcaagc acctctgtta 180

ccccggactg agtatcaata agctgtaccc acggctgaaa gagaaaacgt tcgttatccg 240

gctatctact tcgggaaacc tagtaccatc atgaagattg caaagcgttt cgctcagcac 300

aaccccagtg tagatcaggt cgatgagtca ctgcattccc cacaggcgac tgtggcagtg 360

gctgcgttgg cggcctgcct acggggaaac ccgtaggacg ctttaatgtt aacatggtgc 420

gaagagtcta ctgagctagg tagtagtcct ccggcccctg aatgcggcta atcctaacca 480

cggagcaggt gctcacactc cagtgagtgg cctgtcgtaa cgcgcaagtc tgtggcggaa 540

ccgactactt tgggtgtccg tgtttccttt tatttttatc atggctgctt atggtgacaa 600

tctaagattg ttatcatata gctattggat tggccatccg gtgaactcta gagatattat 660

ttacttgttt gttggtttta tcaagttaaa tatcaccagt tacaccactc tcctttacat 720

tgtactactg aacaatagaa agagaaa 747

<210> 177

<211> 747

<212> DNA

<213> Enterovirus A89 (strain KSYPH-TRMH22F/XJ/CHN/2011)

<400> 177

ttaaaacagc ctgtgggttg tacccaccca cagggcccac tgggcgctag cacactggta 60

tcacggtacc tttgtgcgcc tgttttatgc accctttttc cctgtaactc agaagaaaca 120

aacaaacgat caatagtggg cttagcgctc cagctaagtc ttgatcaagc acttctgtta 180

ccccggaccg agtatcaata ggctgtaccc acggctgaaa gagaaaacgt tcgttatccg 240

gctatctact tcgggaaacc tagtaccatc atgaagattg caaagcgttt cgctcagcac 300

aaccccagtg tagatcaggt cgatgagtca ctgcattccc cacaggtgac tgtggcagtg 360

gctgcgttgg cggcctgcct atggggaaac ccataggacg ctttaatatt gacatggtgc 420

gaagagtcta ctgagctagg tagtagtcct ccggcccctg aatgcggcta atcccaacca 480

cggagcaggc gctcacactc cagtgagtag cttgtcgtaa cgcgaaagtc tgtggcggaa 540

ccgactactt tgggtgtccg tgtttccttt tatttttatc atggctgctt atggtgacaa 600

tctaagattg ttatcatata gctattggat tggccatccg gtgaattcta gagacattat 660

ttacttgttt gttggtttta ccacactgaa cattaccaat cacagaactc tcctttacat 720

cttgttgtta aacaatagaa agagaaa 747

<210> 178

<211> 747

<212> DNA

<213> Human enterovirus A89 (AY697459.1)

<400> 178

ttaaaacagc ctctgggttg cacccaccca cagggcccac tgggcgctag cacactggta 60

tcacggtacc tttgtgcgcc tgttttatac accctccccc cttgtaactt agaagaaaca 120

aacaaacgat caatagtagg cttagcactc cagctaagtc tcgatcaagc acttctgtta 180

ccccggaccg agtatcaata ggctgtaccc acggctgaaa gagaaaacgt tcgttacccg 240

gctatctact tcgggaaacc cagtaccatc atgaagattg caaagcgttt cgctcagcac 300

aaccccagtg tagatcaggt cgatgagtca ctgcattccc cacaggcgac tgtggcagtg 360

gctgcgttgg cggcctgcct atggggaaac ccataggacg ctttaatatt gacatggtgc 420

gaagagtcta ctgagctagg tagtagtcct ccggcccctg aatgcggcta atcccaacca 480

cggagcaggc gctcacactc cagtgagtag cttgtcgtaa tgcgaaagtc tgtggcggaa 540

ccgactactt tgggtgtccg tgtttccttt tatttttatc atggctgctt atggtgacaa 600

tctaagattg ttatcatata gctattggat tggccatccg gtgaattcta gagatattat 660

ttacttgttt gttggtttta ccacattaaa cattaccaat tacagaactc tcctctacat 720

cctgttattg aataatagaa agagaaa 747

<210> 179

<211> 744

<212> DNA

<213> Enterovirus A90 (strain 10-2879-1)

<400> 179

aaacagctct agggttgttc ccaccctagg ggcccacgtg gcggctagca ctctggtatc 60

acggtacctt tgtgcgcctg ttttataccc cctcccctat gtaacttaga agctattaaa 120

taaagttcaa tagaaggggg tacaaaccag tgccaccacg aacaaacact tctgtttccc 180

cggtgaagct acatagactg ttcccacggt cgaaagtagc agatccgtta tccgctttgg 240

tacttcgaga aacctagtat catcttggaa tcttcgatgc gttgcgctca gcactcaacc 300

ccagagtgta gcttaggtcg atgagtctgg acgatcctca ctggcgacag tggtccaggc 360

tgcgttggcg gcctacctgt ggtgaaagcc acaggacgct agttgtgaac aaggtgtgaa 420

gagtctattg agctaccaaa gagtcctccg gcccctgaat gcggctaatc ccaaccacgg 480

agcaagtgct cacaatccag tgagtggctt gtcgtaacgc gcaagtctgt ggcggaaccg 540

actactttgg gtgtccgtgt ttccttttat ttttatcatg gctgcttatg gtgacaatct 600

aagattgtta tcatatagct attggattgg ccatccggtg accagtagag acattatata 660

cttatttgtt ggctttgtta aattagacac aactacactt aaatctcttc tcttcatcac 720

acaattgaat attagaaaaa gaaa 744

<210> 180

<211> 748

<212> DNA

<213> Enterovirus A90 (isolate SCH05F/XJ/CHN/2011)

<400> 180

ttaaaacagc tctgggggtg ttcccaccct agaggcccac gtggcggcta gcactctggt 60

atcacggtac ctttgtgcgc ctgttttata tccctttccc catgcaactt agaagttatt 120

aaacaaagtt caatagaagg gggtacgaac cagtgccacc acgaacaagc acttctgttt 180

ccccggtgaa gctgcataga ctgtccccac ggttgaaagc ggcagatccg ttatccgctt 240

tggtacttcg agaaacccag taccaccttg gaatcttcga tgcgttgcgc tcagcactca 300

accccagagt gtagcttagg ttgatgagtc tggacgatcc tcactggcga cagtggtcca 360

ggctgcgttg gcggcctacc tgtggtgaaa accacaggac gctagatgtg aacaaggtgt 420

gaagagtcta ttgagctacc aaagagtcct ccggcccctg aatgcggcta atcctaacca 480

cggagcaagt acccacaaac cagtgggcag cttgtcgtaa cgcgcaagtc tgtggtggaa 540

ccgactactt tgggtgtccg tgtttccttt ttattttatc atggctgctt atggtgacaa 600

tctaagattg ttatcatata gctattggat tggccatccg gtagactaac agagatatta 660

tatacctatt tgttggtttt attagattga atactatcgc attcaaaact cttctttata 720

tcatacaatt gaatagcaga aagagaaa 748

<210> 181

<211> 749

<212> DNA

<213> Human enterovirus A90 (isolate 01336/SD/CHN/EV90)

<400> 181

tttaaaacag cctgtgggtt gttcccaccc acagggccca ctgggcgcta gcacaccggt 60

atctcggtac ccttgtgcgc ctgttttata cacccttccc cctttgtaac ttagaagaaa 120

tgaacaaatg atcaatagga ggcttagcac accagctagg tcttgatcaa gcacttctgt 180

taccccggac tgagtatcaa taagctgtat ccacggctga aagagaaaac gttcgttatc 240

cggccattta cttcgggaaa cctagtacca tcatgaaggt tgcaaagcgt ttcgctcagc 300

acaaccccag tgtagatcag gtcgatgagt caccgcattc cccacaggcg accgtggcag 360

tggctgcgtt ggcggcctgc ccatggggca acccatagga cgcttcaatg ttgacatggt 420

gtgaagagtc tattgagcta agtagtaatc ctccggcccc tgaatgcggc taatcctaac 480

cacggagcag gcgctcacac tccagtgagt ggcctgtcgt aacgcgcaag tctgtggcgg 540

aaccgactac tttgggtgtc cgtgtttcct tttattttta taatggctgc ttatggtgac 600

aatctaagat tgttatcata tagctattgg attggccatc cggtgaattc tagagatatt 660

atctacttat ttgttggttt taccaagttt aatatcacta gttataccac tctcctttat 720

atcatacaat tgaatagtag aaagagaaa 749

<210> 182

<211> 749

<212> DNA

<213> Human enterovirus A90 (AB192877.1)

<400> 182

tttaaaacag ctctagggtt gttcccgccc tagaggccca cgtggcggct agcactctgg 60

tatcacggta cctttgtgcg cctgttttat accccctccc cccatgtaac ttagaagcta 120

ttaaacaaag ttcaatagaa gggggtacaa accagtacca ccacgaacaa gcacttctgt 180

ttccccggtg aagctacatg gactgttccc acggttgaaa gtggcagatc cgttatccgc 240

tttggtactt cgagaaacct agtatcatct tggaatcttc gacgcgttgc gctcagcact 300

caaccccaga gtgtagctta ggtcgatgag tctggacgat ccccactggc gacagtggtc 360

caggctgcgt tggcggccta cctgtggcga aagccacagg acgctagttg tgaacaaggt 420

gtgaagagtc tattgagcta ccaaagagtc ctccggcccc tgaatgcggc taatcccaac 480

cacggagcaa gtgctcacaa accagtgagt ggcttgtcgt aacgcgcaag tctgtggcgg 540

aaccgactac tttgggtgtc cgtgtttcct tttattttta tcatggctgc ttatggtgac 600

aatctaagat tgttatcata tagctattgg attggccatc cggtgactaa cagagatatc 660

atatatctat ttgttggttt tgttaagcta gacacaactg cattcaattc tcttctcttc 720

atcacacaat tgaataatag aaagagaaa 749

<210> 183

<211> 748

<212> DNA

<213> Human enterovirus A90 (isolate F950027)

<400> 183

tttaaaacag ctctagggtt gttcccaccc tagaggccca agtggcggct agcactctgg 60

tattacggta cctttgtgcg cctgttttat atcccttccc ccatgtaact tagaagatat 120

taaacaaagt tcaataggag ggggtacaaa ccagtgccac cacgaacaaa cacttctgtt 180

tccccggtga agctacatag actgttccca cggttgaaag tggcagatcc gttatccgct 240

ttggtacttc gagaaaccta gtaccacctt ggaatcttcg atgcgttgcg ctcagcactc 300

aaccccagag tgtagcttag gtcgatgagt ctggacgatc ctcactggcg acagtggtcc 360

aggctgcgtt ggcggcctac ctgtggcgaa agccacagga cgctagttgt gaacaaggtg 420

tgaagagtct attgagctac caaagagtcc tccggcccct gaatgcggct aatcccaacc 480

acggagcaag tgcccacaaa ccagtgggtg gcttgtcgta atgcgtaagt ctgtggcgga 540

accgactact ttgggtgtcc gtgtttcctt ttatttttat catggctgct tatggtgaca 600

atctaagatt gttatcatat agctattgga ttggccatcc ggtgactaac agagatcttg 660

catacctgtt tgttggtttt actaaactag atatagttac atttaaaact cttctttata 720

tcatacagtt gaatagtaga aagagaaa 748

<210> 184

<211> 745

<212> DNA

<213> Human enterovirus 91(AY697461.1)

<400> 184

ttaaacagct ctagggttgt tcccacccta gaggcccacg tggcggctag cactctggta 60

tcacggtacc tttgtgcgcc tgttttatat ccctttcccc atgtaactta gaagttatta 120

aacaaagttc aatagaaggg ggtacaaacc agtgccacca cgaacaagca cttctgtttc 180

cccggtgaag ctgtatagac tgtctccacg gttgaaaatg gcagatccgt tacccgcttt 240

ggtacttcga gaaacctagt atcaccttgg aatcttcgat gcgttgcgct cagcactcaa 300

ccccagagtg tagcttaggt cgatgagtct ggacgatcct cactggtgac agtggtccag 360

gctgcgttgg cggcctacct gtggtgaaaa ccacaggacg ctagttgtga acaaggtgtg 420

aagagtctat tgagctacca aagagtcctc cggcccctga atgcggctaa tcctaaccac 480

ggagcaagtg ccttcaaacc agtaggtagc ttgtcgtaac gcgcaagtct gtggcggaac 540

cgactacttt gggtgtccgt gtttcctttt attttatcat ggctgcttat ggtgacaatc 600

taagattgtt atcatatagc tattggattg gccatccggt gactaacaga gatctcatat 660

acctttttgt tggttttact aaattaaata ttaccacgtt caagacactt ctccttatca 720

tacaactaaa tagtgagaag agaaa 745

<210> 185

<211> 702

<212> DNA

<213> Human enterovirus A92 (strain RJG7)

<400> 185

tttaaaacag cctgtgggtt gtacccaccc acagggccca cgcggcgtta gtactctggt 60

atcacggtac ctttgtatgc ctgttttatc tcccttcccc cgtaacgtta gaagaatgaa 120

caaaagacaa ttagcaaatc caccaaccca ggtgggtttt cgtcaagcac ttctgtttcc 180

ccggacctag taccaataag ctgtgcccac ggctgaaggg gaaaacgttc gttatccggc 240

tacctacttc gagaagccta gtaccaccaa tgattgtctc aagtgttacg ctcagcacaa 300

ccccagtgta gttcaggtcg atgagtcacc gaattcccca cgggcgaccg tgtcggtggc 360

tgcgttggcg gcctgcctgc gagtttcact cgcaggacgc ttgattcatg acatggtgcg 420

aagagcctat tgagctaggc agtagtcctc cggcccctga atgcggctaa tcctaaccac 480

ggagccatgc gtcacaaacc agtggcgtca tggtcgtaac gagcaattct gtggcggaac 540

cgactacttt gggtgtccgt gtttcctgtt ttatttacct tggctgctta tggtgacaat 600

ctagagttgt catcatatag cgattggatt ggccatccgg atattacttt gtgtgattta 660

tctcttgctc gtaagcaatt ttaaatagtt tatagcatca tt 702

<210> 186

<211> 632

<212> DNA

<213> Simian enterovirus SV19 (strain NOLA-2)

<400> 186

ggtaccctat cgtcgcctgt tttatttccc ttcccccgta acgttagaag aaagtacaat 60

tgacaaatag taaatccacc aacccaggtg ggttttcgtc aagcacttct gtttccccgg 120

acctagtacc aataagctgt atccacggct gaaggggaaa acgttcgtta cccggctacc 180

tacttcgaga agcctagtac catcattgaa tgtctcaagt gttacgttca gcacaacccc 240

agtgtagttc aggtcgatga gtcaccgaga tccccacggg cgaccgtgtc ggtggctgcg 300

ttggcggcct gcctgtgagt tttgctcaca ggacgcttga ttcatgacat ggtgcgaaga 360

gcctattgag ctaggtagta gtcctccggc ccctgaatgc ggctaatcct aaccacggag 420

ccatgcgttg caaaccagcg acgttgtggt cgtaacgagc aattctgtgg cggaaccgac 480

tactttgggt gtccgtgttt cctgttttac ttaccttggc tgcttatggt gacaattata 540

tagttgtcat catatagcta ttggattggc catccggtta tataaaatca cttattactt 600

gctcaagcaa ttcttatcta atcacagata aa 632

<210> 187

<211> 562

<212> DNA

<213> Simian enterovirus SV19 (isolate cg4006)

<400> 187

ccaccaaccc aggtgggttt tccgtcaagc acttctgtct ccccggactt agtaccaata 60

ggctgtaccc acggccgaaa gggaaaacgt tcgttacccg gctacctact tcgagaagcc 120

tagtaacatc attgaatgtc tcaagtgtta cattcagcac aaccccagtg tagttcaggt 180

cgatgagtca ccgaattccc cacgggcgac cgtgtcggtg gctgcgttgg cggcctgcct 240

gcgggtctca ctcgcaggac gcttgattca tgacatggtg cgaagagcct attgagctag 300

gtagtagtcc tccggcccct gaatgcggct aatcccaacc acggagccat gcgctacaat 360

ccagtggtgt tgtggtcgta acgagtaatt ctgtggcgga accgactact ttgggtgtcc 420

gtgtttcctg ttttatttac cttggctgct tatggtgaca atctagagtt gtcatcatat 480

agcgattgga ttggccatcc ggatatatct tttgtgtgat ttacctcttg ctaccaagca 540

attgtcactt gttaataata cc 562

<210> 188

<211> 700

<212> DNA

<213> Simian enterovirus SV19 (strain M19s (P2))

<400> 188

ttaaaacagc ctgtgggttg tacccacccg cagggcccac tgggcgctag tacactgata 60

tcacggtacc tttgtacgcc tgttttatct ccctaccccc atcgtaactt agaagcttaa 120

caatacagat caatagcctc tgcaccaaac caggtgtgga atgatcaagc atttctgtta 180

ccccggacct agtaccaata ggctgtgtca aacggctgaa ggggaaaaag ttcgttaccc 240

ggccacctac ttcgagaagc ctagtagcat cactgaagct gtgaagcgtt acgatcagca 300

caaccccagt gtagttcagg tcgatgagtc actgcattcc ccacaggcga ctgtagcagt 360

ggctgcgttg gcggcctgcc cttggtctct gaccaaggga cgctttggag tggatatggt 420

gcgaagagcc tattgagcta ggtggtagtc ctccggcccc tgaatgcggc taatcctaac 480

cccggagctg gtgcacacaa accagtgtgt ggccagtcgt aatgagcaat tctgggatgg 540

aaccgactac tttgggtgtc cgtgtttcct gtttcactta ccttggctgc ttatggtgac 600

aattgtatag ttgtcatcat atagctattg gattggccat ccggatatta ttcttgtgtt 660

tatttgtgta atttgatata atttagaacc ctgtataaga 700

<210> 189

<211> 698

<212> DNA

<213> Simian enterovirus SV43 (strain OM112t (P12))

<400> 189

ttaaaacagc ctgtgggttg ttcccaccca cagggcccac tgggcgctag tactctggta 60

ttacggtacc tttgtacgcc tgttttacaa cccttccccc atgtaactta gaagcaacaa 120

ataaacgacc aatagcacac tcaccaatcc agatgagtga tggtcaagca cttctgttac 180

cccgggctta gtaccaatag actgccatcg cggttgaagg ggaaaacgct cgttatccgg 240

ctctctactg cgagaagcct agtaacgcct tagaaatgct caagcgttgc gctcagcaca 300

accccagtgt agctcaggtc gatgagtcac tgcattcccc acgggcgacc gtggcagtgg 360

ctgcgtttgc ggcctgccta cagctctacg ctgtaggacg cttgtgtatg gacatggtgt 420

gaagagccca ttgcgctagg gagtagtcct ccggcccctg aatgcggcta atcctaaccc 480

cggagctggt gcttgtaaac caacaagtgg ccagtcgtaa tgggcaactc tgggatggaa 540

ccgactactt tgggtgtccg tgtttcctgt tttatttacc ttggctgctt atggtgacaa 600

tctagtgttg ttgccatata gcgattggat tggccatccg gatattgttt tagacacttg 660

tgtttgtgag tagatctctt ctgacatagg atataaca 698

<210> 190

<211> 581

<212> DNA

<213> Simian enterovirus SV46 (isolate cg5400)

<400> 190

ggttttcgtc aagcacttct gtttccccgg acttagtacc aataggctgt acccacggct 60

gaaagggaaa acgttcgtta tccggctacc tacttcgaga agcctagtat catcattgaa 120

tgtctcaagt gttacgctca gcacaacccc agtgtagttc aggctgatga gtcaccgaat 180

tccccacggg cgaccgtgtc ggtggctgcg ttggcggcct gcctgcgagt ttcactcgca 240

ggacgcttga ttcatgacat ggtgcgaaga gcctattgag ctaggtagta gtcctccggc 300

ccctgaatgc ggctaatccc aaccacggag catccactga caaaccagtc agcagggtgt 360

cgtaacgggt aactctgtgg cggaaccgac tactttgggt gtccgtgttt cctgttttac 420

ttactctggc tgcttatggt gacaatttac ggattgttac catatagcta ttggattggc 480

catccggatt acaagcaaat aatattattg tttgtgggat ttcttccctt gaatcacact 540

aattacttca ttttattaat tgttttaaag ttagttgcat c 581

<210> 191

<211> 730

<212> DNA

<213> Simian enterovirus SV46 (strain RNM5)

<400> 191

tttaaaacag cctgtgggtt gtacccactc acagggccca cgtggcgtta gtactctggt 60

atcacggtac ctttgtatgc ctgttttatc tcccttcccc cgtaacgtta gaagaataaa 120

caaatgacaa ctagcaaatc caccaaccca ggtgggtttt cgtcaagcac ttctgtttcc 180

ccggacttag taccaatagg ctgtacccac ggctgaaggg gaaaatgttc gttacccggc 240

tacctacttc gaaaagccta gtaccaccaa tgattgtctc aagtgttacg ttcagcacaa 300

ccccagtgta gttcaggccg atgagtcacc gaattcccca cgggcgaccg tgtcggtggc 360

tgcgttggcg gcctgcctgc gggtttcacc cgcaggacgc ttgattcatg acatggtgcg 420

aagagcctat tgagctaggt agtagtcctc cggcccctga atgcggctaa tcctaaccac 480

ggagcattca ctggcaaacc agccagcaga atgtcgtaat gggtaactct gtggcggaac 540

cgactacttt gggtgtccgt gtttcctgtt ttacttattt tggctgctta tggtgacaat 600

ttacggattg ttaccatata gctattggat tggccatccg gattattgca atactactat 660

ttgtgggatt tctccctttg tgttataata actaccccat tctaacatta gctctcaagt 720

ttaatacact 730

<210> 192

<211> 744

<212> DNA

<213> Enterovirus B69 (strain Toluca-1)

<400> 192

ttaaaacagc ctgtgggttg tacccaccca cagggcccac tgggcgctag cacactggta 60

tcacggtacc tttgtgcgcc tgttttatac cccctacccc aactttaaac ttagaagcaa 120

agcaaacccg atcaatagca gatgtagcaa gccagttgca tcttgatcaa gtacttctgt 180

ctccccggac tgagtatcaa tagactgctc acgcggttga aggagaaaac gttcgttacc 240

cgaccaacta cttcgagaaa cctagtaaca ccatgaaagt agcagggtgt ttcgatcagc 300

acaaccccag tgtagatcag gctgatgagt caccgcatcc cccacgggcg accgtggcgg 360

tggctgcgct ggcggcctgc ccatggggca acccatggga cgctctaata tggacatggt 420

gtgaagagtc tattgagcta gttggtagtc ctccggcccc tgaatgcggc taatcctaac 480

tgcggagcac ataccccaaa gccaaggggc agtgtgtcgt aacgggcaac tctgcagcgg 540

aaccgactac tttgggtgtc cgtgtttcct tttattctca aactggctgc ttatggtgac 600

aatctcagag ttgttaccat atagctattg gattggccat ccggtgagca acagagcaat 660

tgtctaccaa tttgttggat ttattccact caatctagcg gttttcagaa cattgaatta 720

tattctaacc ctcaacaaga cgaa 744

<210> 193

<211> 708

<212> DNA

<213> Enterovirus B69 (isolate 15_491)

<400> 193

gcccactggg cgttagcact ctggtattaa ggtacctttg tgcgcctgtt ttataatccc 60

ttcccaactt gcaacttaga agtaacacac tatgaccaat agggggcgtg gcaaaccagc 120

catgccttgg tcaagcactt ctgtttcccc ggactgagta tcaataagct gtccacacgg 180

ctgaaggaga aaacgtccgt tacccggcca gttacttcga gaaacctagt accaccatga 240

aagttgcgta gcgtttcgct ccgcacatcc ccagtgtaga tcaggtcgat gagtcaccgc 300

aatccccacg ggcgaccgtg gcggtggctg cgctggcggc ctgcccatgg ggcaacccat 360

gggacgctac aatactgaca tggtgtgaag agtctattga gctaactggt agtcctccgg 420

cccctgaatg cggctaatcc taactgcgga gcagataccc acacgccagt gggcagtctg 480

tcgtaacggg caactctgca gcggaaccga ctactttggg tgtccgtgtt tccttttatt 540

catattttgg ctgcttatgg tgacaattga gagattgtta ccatatagct attggattgg 600

ccatccggtg acaaacagag ctatcatcta tcttttcatc ggcttcgtac cgctgaacca 660

caaagtattt aacaccctca atttagtatt ggccttcaat atagcaaa 708

<210> 194

<211> 745

<212> DNA

<213> Enterovirus B73 (isolate 088/SD/CHN/04)

<400> 194

tttaaaacag cctgtgggtt gttcccaccc acagggccca ctgggcgcta gcacactggt 60

atcacggtac ctttgtgcgc ctgttttata cacccccccc tacatcgtaa tttagaagtc 120

caagccaaac ggtcaataga caacccagta tgccaactgg gtcccgacca agcacttctg 180

ttgccccgga ccgagtatca ataagctgct tgcgcggctg aaggagaaag cgttcgttac 240

ccggccaatt acttcgagaa acccagtaac accatggaag ttgcgtggcg tttcgctccg 300

cacaacccca gtgtagatca ggtcgatgag tcaccgcaac cctcacgggc gaccgtggcg 360

gtggctgcgt tggcggcctg cccatgggac aacccatggg acgcttcaat accgacatgg 420

tgcgaagagt ctattgagct aattggtatt cctccggccc ctgaatgcgg ctaatcctaa 480

ctgcggagca gacacccaca tgccagtggg cagtctgtcg taacgggaaa ctctgcagcg 540

gaaccgacta ctttgggtga ccgtgtttcc tattatcctt attctggctg cttatggtga 600

caattgaaag attgttacca tatagctatt ggattggcca tccggtgtct aatagagcta 660

ttatatacct ctttgttgga tttataccac ttaacaaagt aaactaccag actctgtgct 720

atatcattgg attgaacacg gtgaa 745

<210> 195

<211> 657

<212> DNA

<213> Human enterovirus B73 (isolate 2776-82)

<400> 195

gcggccccct cccccactgt aacttagaag caatgcatac cgatcaacag tgggcgtggc 60

aaaccagcca tgtcttgatc aaacacttct gtttccccgg actgagtatc aatagactgc 120

tcgcgcggtt gaaggagaaa acgttcgtta tccggccaac tacttcgaga agcttaagta 180

acatcatgaa agttacggag cgtttcgttc agcacatccc cagtgtagat caggccgatg 240

agtcactgct ctccccacgg gcgaccgtgg cagtggctgc gctggcggcc tgcccatggg 300

gtgacccatg ggacgctcta agatggacat ggtgcgaaga gcctattgag ctagttggta 360

gtcctccggc ccctgaatgc ggctaatccc aactgcggag catgtgtctt cactccagaa 420

gatagcatgt cgtaacgggc aactctgcag cggaaccgac tactttgggt gtccgtgttt 480

ccttttattc ctacactggc tgcttatggt gacaattcaa gaattgttac catatagcta 540

ttggattggc catccggtga gcaatagagc aattatctac caatttgttg gatttgtacc 600

cctcgatttt acagccattg gaacacttaa atacatctta gtactcaaca cgacaaa 657

<210> 196

<211> 741

<212> DNA

<213> Human enterovirus 74 (strain Rikaze-136/XZ/CHN/2010)

<400> 196

ttaaaacagc tctggggttg ttcccaccca cagggcccac tgggcgctag catactggta 60

tcacggtacc tttatgcgcc tgttttatcc acccctcccc aacgtaacct agaagacact 120

catggatgat caataggagg ctcggcatgc cagctgagtc tggatcaagc acttctgttg 180

ccccggaccg agtatcaata ggctgctcac gcggctgaag gagaaaaagt tcgttatccg 240

gccaattact tcgagaaacc cagtaccacc atgaaggttg cgtggcgttt cgctccgcac 300

agccccagtg tagatcaggt cgatgagtca ccgcgttccc cacgggcgac cgtggcggtg 360

gctgcgttgg cggcctgccc atggggtaac ccatgggacg cttcaatact gacatggtgc 420

gaagagtcta ttgagctaat tggtagtcct ccggcccctg aatgcggcta atcctaactg 480

cggagcagac gtccacaaac cagtgggtgg tctgtcgtaa cgggcaactc tgcagcggaa 540

ccgactactt tgggtgtccg tgtttccttt attcttatat tggctgctta tggtgacaat 600

tgagagattg ttgccatata gctattggat tggccatccg gtttgcaata gagcgattat 660

actcctcttt gttgggtttg tacctctcaa cctcaatagt tacaggacat tattatacat 720

tatccagctg aacacgaaaa g 741

<210> 197

<211> 743

<212> DNA

<213> Enterovirus B75 (isolate Y16/XZ/CHN/2007)

<400> 197

tttaaaacag ctctggggtt gttcccaccc acagggccca tcgggcgcta gcacactggt 60

attacggtac ctttgtgcgc ctgttttata cgcccttccc ccgtgtaact tagaagcaca 120

tcctacgcgg tcgatagata gctcagcaat ccagctgggc tctgaccaag cacttctgtt 180

accccggacc gagtatcaat aggctgctca cgcggctgaa ggagaaaacg ttcgttaccc 240

ggccaattac ttcgagaaac ctagtaccgc catgaaagtt gcgaagcgtt tcgctccaca 300

caaccccagt gtagatcagg tcgatgagtc accgcaatcc ccacgggcga ccgtggcggt 360

ggctgcgttg gcggcctgcc catggggcaa cccatgggac gcttcaatac cgacatggtg 420

cgaagagtct attgagctaa ttggtagtcc tccggcccct gaatgcggct aatcctaact 480

gcggagcaga tactcgcaaa ccagcgggca gtctgtcgta acgggtaact ctgcagcgga 540

accgactact ttgggtgtcc gtgtttccct ttattcttat gctggctgct tatggtgaca 600

attgaagaat tgttaccata tagctattgg attggccatc cagtgacaag tagagcgatc 660

atttacctat ttgttggctt tgtaccattg aattttaaag ccttgaaaac cttaaatctc 720

gttatattgc tcaacgctac aaa 743

<210> 198

<211> 744

<212> DNA

<213> Enterovirus B75 (isolate 102/SD/CHN/97)

<400> 198

tttaaaacag cctgtgggtt gtttccaccc acagggccca cagggcgcta gcactttggt 60

atcacggtac ctttgtgcgc ctgttttata acccttcccc cccacagaaa cttagaagca 120

ccacacaccg atcaatagaa agcgtggcgc accagccatg tcttgatcaa gcacttctgt 180

ttccccggac cgagtatcaa tagactgctc gcgcggtcga aggagaaaac gtccgttatc 240

cggctgacta cttcgagaaa cccagtaaca ccatggaaat tgcggagtgt ttcgatcagc 300

acatccccag tgtagatcag gtcgatgagt caccgcattc cccacgggtg accgtggcgg 360

tggctgcgtt ggcggcctgc ctacggggca tcccgtagga cgcttcaaaa tggacatggt 420

gcgaagagtc tattgagcta gtcagtagtc ctccggcccc tgaatgcggc taatcctaac 480

tgcggagcgc gcgcctccaa cccagggggt agcgcgtcgt aacgggtaac tctgcagcgg 540

aaccgactac tttgggtgtc cgtgtttctt tttatcatta cattggctgc ttatggtgac 600

aattgaaagg ttgttaccat atagctattg gattggccac ccggtgtcta atagagcgat 660

tgtgtacctt tttgttgggt ttgtgccact ttgtaaaaca gcctatcaga ctttgtgctt 720

tatagtcaaa ctaaatacag caaa 744

<210> 199

<211> 747

<212> DNA

<213> Enterovirus B75 (strain USA/OK85-10362)

<400> 199

tttaaaacag tcctgtgggt tgttacccac ccacagggcc cactgggcgc cagcacactg 60

gtatcacggt acctttgtgc gcctgtttta tatacccttc ccctactaag taacttagaa 120

gcaattaaac aaagaccaac agtaagcaca gcgcaccagc tgtgtttcgg tcaagcactt 180

ctgttacccc ggactgagta tcaataggct actcacgtgg ctgaaggaga aaacgttcgt 240

tacccgacca attacttcga gaagcctagt aacaccatgg aggttgcgcg gcgtttcgct 300

ccacacaacc ccagtgcaga tcaggtcgat gagccaccgc attccccacg ggcgaccgtg 360

gcggtggctg cgttggcggc ctgcccatgg ggcaacccat gggacgcttc aatactgaca 420

tggtgcgaag agtctattga gctaattggt agtcctccgg cccctgaatg cggctaatcc 480

caactgcgga gcagataccc acgcgccagt gggcagtctg tcgtaatggg caactctgca 540

gcggaaccga ctactttggg tgtccgtgtt tcctttattc ttatactggc tgcttatggt 600

gacaattgag agattgttac catatagcta ttggattggc catccggtga cgagcagagc 660

tattatttac ctatttgttg gttttatacc attgaactat aaagttttaa aaaccttaaa 720

cttcatctta gtactcaata cagcaaa 747

<210> 200

<211> 749

<212> DNA

<213> Human enterovirus B77 (strain USA/TX97-10394)

<400> 200

ttaaaacagg cttgttgggt tgttcccacc cacaaggccc actgggcgct agcacactgg 60

tattccggta cctttgtgcg cctgttttat accccctccc ccccccgtaa caaacttaga 120

agcaaagtaa actcgatcaa tagccggtgt gacaaaccag tcgcatcttg atcaagcact 180

tctgtctccc cggaccgagt atcaataaac tgctcacgcg gttgaaggag aaagcgttcg 240

ttacccgacc aattacttcg aaaaacctag taccaccatg aaagttgcgg ggtgtttcgc 300

tcagcacaac cccagtgtag atcaggtcga tgagtcaccg cattccccac gggcgaccgt 360

ggcggtggct gcgctggcgg cctgcctatg gggcaaccca taggatgctc taaaacggac 420

atggtgcgaa gagtctattg agctagttgg tagtcctccg gcccctgaat gcggctaatc 480

ctaactgcgg agcaggcacc tacaagccag tgggcagcct gtcgtaacgg gcaactctgc 540

agcggaaccg actactttgg gtgtccgtgt ttccttttac ctcaattttg gctgcttatg 600

gtgacaattg aaagattgtt accatatagc tattggattg gccatccggt gactaacaga 660

gctattgtgt accttttcgt tggttttata ccactcaccc tcgcggaggt taagatttta 720

ctttatataa tacacttgaa cacattaaa 749

<210> 201

<211> 742

<212> DNA

<213> Human enterovirus B77 (strain CF496-99)

<400> 201

ttaaaacagc ctgtgggttg tacccaccca cagggcccat tgggcgctag cacactggta 60

ttccggtacc cttgtgcgcc tgttttatct acccttccct actgcaactt agaagttaaa 120

cagtcacggt cgataggcgg ttcagtaaac caactggatc ttgaccaagc acttctgtta 180

ccccggactg agtatcaata agctgcttac gtggctgaag gagaaaacgt tcgttacccg 240

gccaattact tcgagaaacc tagtaccacc atgaaggttg cgcagcgttt cgctccgcac 300

aaccccagtg tagatcaggt cgatgagtca ccgcactcct cacgggcgac cgtggcggtg 360

gctgcgctgg cggcctgccc atggggcaac ccatgggacg cttcaatact gacatggtgc 420

gaagagtcta ttgagctaat tggtaatcct ccggcccctg aatgcggcta atcccaactg 480

cggagcaggt acccacacac cagtgggcag cctgtcgtaa cgggcaactc tgcagcggaa 540

ccgactactt tgggtgtccg tgtttccttt tattcctata ctggctgctt atggtgacaa 600

ttgaaagatt gttaccatat agctattgga ttggccatcc agtgacaaac agagcaattg 660

tctatctatt tgtcggtttt gtaccaataa atttcaaagt cctaaaaacc ctcaatttca 720

tattgttact taacacaaca ag 742

<210> 202

<211> 733

<212> DNA

<213> Human enterovirus B79 (strain 17-2255-1_E79)

<400> 202

ctgtgggttg ttcccaccca cagggcccac tgggcgccag cactctgatt ccacggaatc 60

tttgtgcgcc tgttttacaa ccctccccaa tttgtaacgt agaagcaata cacactactg 120

atcaatagta ggcatggcgc gccagtcatg tcatgatcaa gcacttctgt tccccggact 180

gagtatcaat agactgctca cgcggttgaa ggagaaaacg ttcgttaccc ggctaactac 240

ttcaagaaac ctagtagcac catggaagct gcggagtgtt tcgctcagca cttcccccgt 300

gtagatcagg tcgatgagtc actgcaatcc ccacgggcga ccgtggcagt ggctgcgttg 360

gcggcctgcc tatggggtaa cccataggac gctctaatgt ggacatggtg cgaagagtct 420

attgagctag ttagtagtcc tccggcccct gaatgcggct aatcctaact gcggagcaca 480

tgccttcaac ccaggaggtg gtgtgtcgta acgggtaact ctgcagcgga accgactact 540

ttgggtgtcc gtgtttcctt ttatccttat attggctgct tatggtgaca atcacggaat 600

tgttgccata tagctattgg attggccatc cggtgtctaa cagagctatt gtatacctat 660

ttgttggatt tgtaccacta aattacaagg ttttaaacac tctcaacctt ataatactac 720

tcaactcagc aaa 733

<210> 203

<211> 733

<212> DNA

<213> Human enterovirus B79 (AB426610.1)

<400> 203

ctgtgggttg ttcccaccca cagggcccac tgggcgccag cactctgatt ccacggaatc 60

tttgtgcgcc tgttttacaa ccctccccaa tttgtaacgt agaagcaata cacactactg 120

atcaatagta ggcatggcgc gccagtcatg tcatgatcaa gcacttctgt tccccggact 180

gagtatcaat agactgctca cgcggttgaa ggagaaaacg ttcgttaccc ggctaactac 240

ttcaagaaac ctagtagcac catggaagct gcggagtgtt tcgctcagca cttcccccgt 300

gtagatcagg tcgatgagtc actgcaatcc ccacgggcga ccgtggcagt ggctgcgttg 360

gcggcctgcc tatggggtaa cccataggac gctctaatgt ggacatggtg cgaagagtct 420

attgagctag ttagtagtcc tccggcccct gaatgcggct aatcctaact gcggagcaca 480

tgccttcaac ccaggaggtg gtgtgtcgta acgggtaact ctgcagcgga accgactact 540

ttgggtgtcc gtgtttcctt ttatccttat attggctgct tatggtgaca atcacggaat 600

tgttgccata tagctattgg attggccatc cggtgtctaa cagagctatt gtatacctat 660

ttgttggatt tgtaccacta aattacaagg ttttaaacac tctcaacctt ataatactac 720

tcaactcagc aaa 733

<210> 204

<211> 745

<212> DNA

<213> Human enterovirus B79 (strain USA/CA79-10384)

<400> 204

ttaaaacagc ctgtgggttg tacccaccca cagggcccac tgggcgctag cacactggta 60

tcccggtacc tttgtgcgcc tgttttatac cccctccccc atatcgtaac atagaagcaa 120

tgcactcccg gtcaatagct gacgtgacaa accagttgcg ttttgaccaa gcacttctgt 180

aaccccggac cgagtatcaa taagctgctt gcgcggctaa aggagaaacc gtccgttacc 240

cggctaacta cttcgagaaa cctagtacca ccatgaacgt tgcggagtgt ttcgctcagc 300

acttcccccg tgtagatcag gtcgatgagc caccgcattc cccacgggcg accgtggcgg 360

tggctgcgtt ggcggcctgc ctgtggggca acccacagga cgctctaata tagacatggt 420

gtgaagagtc tattgagcta gttagtagtc ctccggcccc tgaatgcggc taatcctaac 480

tgcggagcac atacccctaa tccaaggggc agtgtgtcgt aacgggcgac tctgcagcgg 540

aaccgactac tttgggtgtc cgtgtttcct tttatctttt atattggctg cttatggtga 600

caattgcaga gttgttacca tatagctttt ggattggcca tccggtgagc aatagagctg 660

ttatttatca atttgttgga ttcgtcgcac tcaaccacac agtctttaga acactcggat 720

ttatcttgac ccttaatacg agaaa 745

<210> 205

<211> 742

<212> DNA

<213> Enterovirus B80 (isolate HT-LYKH203F/XJ/CHN/2011)

<400> 205

ttaaaacagc tctggggttg ttcccaccca cagggcccac cgggcgccag cactctggta 60

tcacggtacc tttgtgcgcc tgttttatac ccccgtcccc ggttgtaact tagaagtaac 120

actccacgac cgatagtagg tgtggcacgc cagccatgtc ttggtcaagc acttctgttt 180

ccccggattg agtatcaata ggctgttcac acggccgaag gagaaaacat ccgttatccg 240

gccaactact tcgagaaacc tagtaccacc atgaaggttg cgcagtgttt cgctccgcac 300

aaccccagtg tagatcaggc cgatgagtca ccgctttccc cacgggcgac cgtggcggtg 360

gctgcgttgg cggcctgccc atggggaaat ccatgggacg cttcaatact gacatggtgc 420

gaagagtcta ttgagctagt tggtattcct ccggcccctg aatgcggcta atcctaactg 480

cggagcagac acccacatgc cagtgggcag tctgtcgtaa cgggcaactc tgcagcggaa 540

ccgactactt tgggtgtccg tgtttccttt tattcttata ctggctgctt atggtgacaa 600

ttgaaagatt gttaccatat agctattgga ttggccatcc tgtgactaac agagctattg 660

tatacctatt tgttggtttt gtgccactga attacaaggc tttaaacacc ctcaacttta 720

taatactact caactcatta aa 742

<210> 206

<211> 742

<212> DNA

<213> Human enterovirus B80 (isolate HZ01/SD/CHN/2004)

<400> 206

ttaaaacagc ctgtgggttg atcccaccca cagggcccac tgggcgctag cacactggta 60

tcccggtacc tttgtgcgcc tgttttattt acccttcccc aatgtaattt agaagtttat 120

cccaaatggt caatagacaa cccagcacgc cagctgggct tcgaccaagc acttctgtta 180

ccccggaccg agtatcaata ggctgctcac gcggctgaaa gagaaaatgt tcgttacccg 240

gccaaccact tcgagaaact tagtatcacc atgaaagttg cgcagtgttt cgctccgcac 300

agccccagtg tagatcaggt cgatgagtca ccgcgttccc cacgggcgac cgtggcggtg 360

gctgcgttgg cggcctgccc atggggcaac ccatgggacg cttcaatact gacatggtgc 420

gaagagtcta ttgagctggt tggtagtcct ccggcccctg aatgcggcta atcctaactg 480

cggagcagat acctacacac cagtgggcag tctgtcgtaa cgggcaactc tgcggcggaa 540

ccgactactt tgggtgtccg tgtttccttt tattcttata ttggctgctt atggtgacaa 600

ttgaaagatt gttaccatat agctattgga ttggccatcc agtgacaaac agagctatcg 660

tgtacctatt tgttggtttt gtaccactga accgtaaggt gttagacact ctaaacctca 720

taattctgtt caattcagca aa 742

<210> 207

<211> 741

<212> DNA

<213> Enterovirus B81 (isolate 99279/XZ/CHN/1999)

<400> 207

ttaaaacagc tctggggttg ctcccaccca cagggcccaa tgggcgctag cacactagta 60

ttgaggtact tttgtgcgcc tgttttatcc accctctccc cacgtaactt agaagcaaaa 120

ctcattcggt caatagacag cccagtaagc caactgggca atgaccaagc acttctgttc 180

cccggactga gtatcaatag actgctcacg cggttgaagg agaaatcgtt cgttacccgg 240

ccaattactt cgagaaacct agtaccacca tggagattgc gaagtgtttc gctccgcaca 300

accccagtgt agatcaggct gatgagtcac cgcattcctc acgggcgacc gtggcggtgg 360

ctgcgctggc ggcctgccca tggggcaacc catgggacgc ttcaatatta acatggtgtg 420

aagagtctat tgagctaatt ggtagtcctc cggcccctga atgcggctaa tcctaactgc 480

ggagcagaca cccacacgcc agtgggcagt ctgtcgtaac gggaaactct gcagcggaac 540

cgactacttt gggtgtccgt gtttcctttt attcttatat tggctgctta tggtgacaat 600

tgaaagattg ttaccatata gctattggat tggccatcca gtgacaaatc gagcgatcat 660

ttacttgttt gttggtttcg tcccgctaaa ctttaaagtt gtgaatactc ttaactttat 720

tatagcacag aacacatcac t 741

<210> 208

<211> 739

<212> DNA

<213> Human enterovirus B81 (strain USA/CA68-10389)

<400> 208

ttaaaacagc ctgtgggttg tacccaccca cagggcccat tgggcgcttg cacactggta 60

ttccggtacc tttgtgcgcc tgttttatac atcctacccc cgtaacttag aagcaagatc 120

acccgatcac caggaaatgt ggcaaaccag tcacattctg atcaagcact tctgtttccc 180

cggactgagt atcaataggc tgttcacacg gctgaaggag aaaacgttcg ttacccggcc 240

aactacttcg aaaaacctag tatcaccgtg aacgttgcag agtgtttcgc tcagcacttt 300

ccccgtgtag atcaggtcga tgagtcaccg cattccccac gggcgaccgt ggcggtggct 360

gcgttggcgg cctgcctgcg gggcaacccg taggacgctc taatacagac acggtgtgaa 420

gagtctattg agctagttgg tagtcctccg gcccctgaat gcggctaatc ctaactgcgg 480

agcgagcgct tgtaatccaa caggtagctc gtcgtaacgg gtaactctgc agcggaaccg 540

actactttgg gtgaccgtgt ttccttttat ttcattactg gctgcttatg gtgacaatca 600

cagagttgtt accatatagc tattggattg gccatccggt gtcaaataga gcaattgtgt 660

ttctttttgt tgggtttgta ccacttgact tgaccagcta caacactctg tgctacattg 720

ttagattgaa cacagcaaa 739

<210> 209

<211> 745

<212> DNA

<213> Human enterovirus B82 (strain USA/CA64-10390)

<400> 209

ttaaaacagc ctgtgggttg cacccacccg cagggcccac tgggcgctag tacactggta 60

ccccggtacc cttgtacgcc tgttttattt ctccctcccc caaccagtaa cctagaagta 120

aagcaaaccc gatcaatagc aggtgcgaca caccagtcgt atcttgatca agcacttctg 180

taaccccggg ccgagtatca atagactgct cacgcggttg agggagaaaa cgctcgttat 240

ccggctgact acttcgagaa acccagtaac accatagaag ttgcagagtg tttcgctcag 300

cactttcccc gtgtagatca ggtcgatgag tcaccgcatt ccccacgggt gaccgtggcg 360

gtggctgcgt tggcggcctg cctatggggc aacccatggg acgctctaat acagacatgg 420

tgtgaagagt caattgagct agtcagtatt cctccggccc ctgaatgcgg ctaatcctaa 480

ctgcggagca cgtgcctcca atccaggggg tggcgtgtcg taacgggtaa ctctgcagcg 540

gaaccgacta ctttgggtgt ccgtgtttcc ttttattctt atcttggctg cttatggtga 600

caattgaagg attgttacca tatagctatt ggattggcca tccggtgact aacagagcaa 660

tcatatacca atttgttggt tttataccat tgaattacaa agttctaatc acccttaaat 720

tcattttaac acttaatacg tcaaa 745

<210> 210

<211> 742

<212> DNA

<213> Human enterovirus B83 (strain USA/CA76-10392)

<400> 210

tttaaaacag cctgtgggtt gttcccaccc acagggccca ctgggcgcta gcactctggt 60

atcacggtac ccttgtgcgc ctgttttata tccccacccc agtggaacat tagaagcagc 120

acacctatga tcaatagtgg gcgtggcatc cagtcacgtt atgatcaagc acttctgtga 180

ccccggactg agtatcaata gactgctcac gcggttgaag gagaaaacgt tcgttacccg 240

gccagttact tcgagaaacc cagtaacacc atgaatgttg cagagtgttt cgctcagcac 300

ttcccccgtg tagatcaggt cgatgagcca ctgcattccc cacgggcgac cgtggcagtg 360

gctgcgttgg cggcctgcct atggggtcac ccataggacg ctctaatact gacatggtgc 420

gaagagtcta ttgagctaac tggtagtcct ccggcccctg aatgcggcta atcctaactg 480

tggagcacat tcccccaatc cagggggcag tgtgtcgtaa cgggcaactc tgcagcggaa 540

ccgactactt tgggtgtccg tgtttctttt tattctcata ttggctgctt atggtgacaa 600

ttacagaatt gttaccatat agctattgga ttggccatcc ggtgactaac agagcaatta 660

tttatcaatt cgttggattt gtcccactcg atttcaaagc agtcaataca ctctcatata 720

tcgttacact caacacaaca aa 742

<210> 211

<211> 744

<212> DNA

<213> Enterovirus B83 (isolate 99245/XZ/CHN/1999)

<400> 211

tttaaaacag ctctggggtt gttcccacct acagggccca ttgggcgcta gcactctggt 60

atcacggtac ccttgtgcgc ctgttttaca gcccctcccc cgaacatgaa cttagaagca 120

ccacacaccg atcaacagcg ggcgtagcat accagctacg tcttgatcaa gcacttctgt 180

ttccccggac tgagtatcaa tagactacta acgtggtcga aggagaaaac gttcgttacc 240

cggccagcta cttcgaaaag cctagtatca ccatggaaat tgcggagtgt ttcgctcagc 300

gcacccccag cgtagatcag gccgatgagt caccgcgtcc ctcacgggcg accgtggcgg 360

tggctgcgtt ggcggcctgc ctacggggca acccgtagga cgcttcaata tggacatggt 420

gcgaagagcc cattgagcta gttggtagtc ctccggcccc tgaatgcggc taatcctaac 480

tgcggagcgc tcgcctccaa accagggggt tgtgcgtcgt aatgggtaac tctgcagcgg 540

aaccgactac tttgggtgtc cgtgtttcct tttattttta tcatggctgc ttatggtgac 600

aattgaaagg ttgttaccat atagctattg gattggccat ccagtgtcta acagagcaat 660

tatatacctc tttgttggat ttgtaccgct aaacaagaca aattatcaaa ctttgtgcct 720

tattgttaaa ttaaacacaa caag 744

<210> 212

<211> 744

<212> DNA

<213> Enterovirus B83 (isolate AFP341-GD-CHN-2001)

<400> 212

tttaaaacag cctgtgggtt gttcccaccc acagggccca ctgggcgcta gcactctggt 60

atcacggtac ctttgtgcgc ctgttttata gcccctccct tagataggaa tttagaagca 120

ctgcacaccg atcaatagca ggcgcggcac gccagccacg tcttgatcaa gcacttctgt 180

ttccccggac cgagtatcaa tagactgtta acgcggtcga aggagaaaac gttcgttatc 240

cggccaacta cttcgagaaa cctagtaacg ccatggaagt tgcggagtgt ttcgctcagc 300

acatccccag tgtagatcag gtcgatgagt caccgcatcc ctcacgggtg accgtggcgg 360

tggctgcgtt ggcggcctgc ctacggggca acccgtagga cgcttcaata tggacatggt 420

gcgaagagta tattgagcta gttggtagtc ctccggcccc tgaatgcggc taatcctaac 480

tgcggagcgc tcgcccccaa tccagggggt cgtgcgtcgt aacgggtaac tctgcagcgg 540

aaccgactac tttgggtgtc cgtgtttcct tttatcttta tcacggctgc ttatggtgac 600

aattgaaaga ttgttaccat atagctattg gattggccat cctgtgtcta acagagcaat 660

catatatctt tttgttggat ttgtgccact tgataaaata agttaccaga ctttgtgttt 720

tatcatcaga ctaaacacaa caaa 744

<210> 213

<211> 742

<212> DNA

<213> Enterovirus B83 (isolate 246/YN/CHN/08)

<400> 213

tttaaaacag cctgtgggtt gttcccaccc acagggccca ctgggcgtaa gcacactggt 60

atcacggtac ctttgtgcgc tgttttatcc acccctcccc aatgtaactt agaagcacta 120

aatgaatggt caacaggggg cccagcacac cagttgagtc ctgaccaagc acttctgtca 180

ccccggactg agtatcaata agctactcac gtggctgaag gagaaaacgt tcgttacccg 240

gccaattact tcgagaaact tagtaccacc atgaaggttg cgcagcgttt cgctccacac 300

aaccccagtg tagatcaggc cgatgagtca ccgcattccc cacgggcgac cgtggcggtg 360

gctgcgctgg cggcctgccc gaggggcaac cctcgggacg cttcaatacc gacatggtgc 420

gaagagtcta ttgagctaat tggtagtcct ccggcccctg aatgcggcta atcctaactg 480

cggagcagat acccacacgc cagtgggcag tctgtcgtaa tgggtaactc tgcagcggaa 540

ccgactactt tgggtgtccg tgtttccttt tattcttaca ctggctgctt atggtgacaa 600

ttgagagatt gttaccatat agctattgga ttggccatcc ggttagtaac agagcgattg 660

tttacttgtt tgttggtttt atacccttga acctcagagt acttgaaaca cttgatttta 720

taataaaatt gaacacaaca aa 742

<210> 214

<211> 726

<212> DNA

<213> Enterovirus B84 (strain GHA:BAR:TES/2017)

<400> 214

ggttgttccc acccacaggg cccattgggc gctagcacac tggtatcacg gtacccttgt 60

gcgcctgttt tatacaccct tccccaacga aacttagaag taaagcaaca aggtcaatag 120

gtagctcagt gagccaactg agttatgacc aagcacttct gtcaccccgg actgagtatc 180

aataggctgc tcacgcggct gaaggagaaa gcgttcgtta tccggctaac tacttcgaga 240

aacctagtac caccatgaag gttgcgtagc gtttcgatca gcacaacccc ggtgtagatc 300

aggccgatga gtctccgcat tcctcacggg cgaccgtggc ggtggctgcg ctggcggcct 360

gcccatgggg caacccatgg gacgcttcaa tactgacatg gtgcgaagag tctattgagc 420

tagttagtag tcctccggcc cctgaatgcg gctaatccta actgcggagc acacaccccc 480

aacccaggag gcagtgtgtc gtaacgggta actctgcagc ggaaccgact actttgggtg 540

tccgtgtttc cttttattct tatactggct gcttatggtg acaattgaga gattgttacc 600

atatagctat tggattggcc atccggtgac taacagagct attgtatacc tgtttgttgg 660

ttttatacca ttaaattgca aagttctaga gaccctcaat tttatcctat tactaaacgc 720

agcaaa 726

<210> 215

<211> 742

<212> DNA

<213> Enterovirus B84 (isolate AFP452/GD/CHN/2004)

<400> 215

ttaaaacagc ctgtgggttg ttcccaccca cagggcccac tgggcgctag cacactggta 60

tcacggtacc cttgtgcgcc tgttttatca accccttccc gatgtaactt agaagccaaa 120

tcttaccggt caacaggtgg tccagtatgc caactgggct ctgaccaagc acttctgtta 180

ccccggactg agtatcaata ggctgctcac gcggctgaag gagaaaatgt ccgttacccg 240

gccaattact tcgagaaacc tagtaccacc atgaaggttg cgcggcgttt cgctccacac 300

aaccccagtg cagatcaggt cgatgagcca ccgcttcccc cacgggcgac cgtggcggtg 360

gctgcgttgg cggcctgccc atgggacaac ccatgggacg cttcaatact gacatggtgc 420

gaagagtcta ttgagctaat tggtagtcct ccggcccctg aatgcggcta atcctaactg 480

tggagcagat gcccgcaagc cagcgggtgg tctgtcgtaa cgggcaactc cgcagcggaa 540

ccgactactt tgggtgaccg tgtttccttt tatttttata ctggctgctt atggtgacaa 600

ttgaaagatt gttaccatat agctattgga ttggccatcc ggtgactaac agagctatca 660

tatatctatt tgttggttac gtcccacttg actatagagt tctacgcacc ttatatttca 720

ttttagatct gaacaaagcg ag 742

<210> 216

<211> 744

<212> DNA

<213> Human enterovirus B84 (isolate CIV2003-10603)

<400> 216

ttaaaacagc ctgtgggttg ttcccaccca cagggcccac cgggcgccag cacactggta 60

tcacggtacc cttgtgcgcc tgttttatcc accccttccc acagtaactt agaagcacac 120

catgtatacg gtcaataggc ggctcagtac accaactggg ccacgaccaa gcacttctgt 180

taccccggac tgagtatcaa taagctgctc acgcggctga aggagaaaac gttcgttacc 240

cggccaatta cttcgagaaa cccagtacca ccatgaaggt tgcgcagcgt ttcgttccgc 300

acaaccccag tgtagatcag gtcgatgagt caccgcgttc ctcacgggtg accgtggcgg 360

tggctgcgtt ggcggcctgc ccatggggca acccatggga cgcttcaata ctgacatggt 420

gcgaagagtc tattgagcta attggtagtc ctccggcccc tgaatgcggc taatcctaac 480

tgcggagcag atacccacaa accagtgggc agtctgtcgt aacgggcaac tctgcagcgg 540

aaccgactac tttgggtgtc cgtgtttcct tttatcctta cactggctgc ttatggtgac 600

aattgagaaa ttgttaccat atagctattg gattggccat ccggtgacaa acagagcagt 660

tgtttaccta tttgttggtt tcgtaccgct gaaccttaaa gttctaaaga ccctcaattt 720

tatcttagca cttaacacag caaa 744

<210> 217

<211> 744

<212> DNA

<213> Human enterovirus B85 (strain HTPS-MKLH04F/XJ/CHN/2011)

<400> 217

ttaaaacagc ctgtgggttg ttcccaccca cagggcccac tgggcgctag cacactggta 60

ttacggtacc tttgtgcgcc tgttttatat acccccccca agtgcaactt agaagcaaga 120

catgaccggt cagcaggtga ttcagtaaac caactgagtc ttgaccaagc acttctgttt 180

ccccggaccg agtatcaata ggctgcttac gcggctgaag gagaaagtgt tcgttacccg 240

gccaattact tcgagaagcc tagtaccacc atggaggttg cgcggcgttt cgctccgcac 300

acccccagtg tagatcaggc cgatgagcca ccgcattccc cacgggtgac cgtggcggtg 360

gctgcgttgg cggcctgccc atggggtaac ctatgggacg cttcaatact gacatggtgc 420

gaagagccta ttgagctaat tggtagtcct ccggcccctg aatgcggcta atcctaactg 480

cggagcaggt acccacatac cagtgggcag cctgtcgtaa cgggtaactc tgcagcggaa 540

ccgactactt tgggtgaccg tgtttccttt tattttttcc tattggctgc ttatggtgac 600

aatcgaggaa ttgttgccat atagctattg gattggccat ccggtgtcaa atagagcgat 660

tatttacctg tttgttggat ttgtaccact gaactttaag gcgctcatca ctctcaacta 720

tattttatcc cttaacgcaa caaa 744

<210> 218

<211> 742

<212> DNA

<213> Human enterovirus B85 (strain BAN00-10353)

<400> 218

ttaaaacagc ctgtgggttg ttcccaccca cagggcccac cgggcgctag cacactggta 60

ttgcggtacc tttgtgcgcc tgttttatac acccctcctt aatgtaactc agaagtacaa 120

cattaccggt caacagatga ctcagtaaac caactgagcc tcgaccaagc acttctgttt 180

ccccggaccg agtatcaata ggctgctcac gcggctgaag gagaaagtgt tcgttacccg 240

gccaattact tcgagaagcc cagtaccacc atggaggttg cgcggcgttt cgctccgcac 300

atccccagtg tagatcaggc tgatgagtca ccgcgttccc cacgggtgac cgtggcggtg 360

gctgcgctgg cggcctgccc atggggtaac ccatgggacg cttcaatact gacatggtgt 420

gaagagccta ttgagctaat tggtagtcct ccggcccctg aatgcggcta atcctaactg 480

cggagcagat acccacacac cagcgggcag tctgtcgtaa cgggcaactc tgcagcggaa 540

ccgactactt tgggtgtccg tgtttccttt tatttttatg ctggctgctt atggtgacaa 600

ttgaggaatt gttaccatat agctattgga ttggccatcc agtgtccaac agagcgatca 660

tttacctgtt cgttgggttc gtaccactaa accacaagtc actcagcaca cttaattaca 720

ttataactct caacacaaca ag 742

<210> 219

<211> 742

<212> DNA

<213> Human enterovirus B86 (strain BAN00-10354)

<400> 219

ttaaaacagc ctgtgggttg tacccaccca cagggcccac tgggcgctag cacactggta 60

ttacggtacc cttgtgcgcc tgttttattt acccctcccc cacgcaacct agaagcatga 120

cttaagcggt caatagatgg ctcagtatgc caactgagtt atgaccaagc acttctgtta 180

ccccggaccg agtatcaata ggctgctcac gcggctgaag gagaaagtgt tcgttacccg 240

gccagttact tcgagaaacc tagtatcatc atgaaggttg cgaggcgttt cgctccacac 300

gaccccggtg tagatcaggc tgatgagtca ccgcattccc cacgggcgac cgtggcggtg 360

gctgcgctgg cggcctgccc atggggcaac ccatgggacg cttcaatatt gacatggtgc 420

gaagagtcta ttgagctaac tggtagtcct ccggcccctg aatgcggcta atcctaactg 480

cggagcagac acccacatgc cagtgggcag tctgtcgtaa cgggcaactc tgcagcggaa 540

ccgactactt tgggtgtccg tgtttccttt tattcttata ttggctgctt atggtgacaa 600

ttgaaagatt gttgccatat agctattgga ttggccatcc agtgactaac agagcaattg 660

tctacctgtt cgttggttac gtaccactaa attataaagt tttgaacaca cttaatttta 720

ttatagcact taacacctta aa 742

<210> 220

<211> 743

<212> DNA

<213> Enterovirus B87 (isolate LY02/SD/CHN/2000)

<400> 220

ttaaaacagc ctgtgggttg ttcccaccca cagggcccat agggcgccag cactctggta 60

tcccggtacc tttgtgcgcc tgttttatcc acccttcccc aagcgtaact tagaagccca 120

tcatacacgg tcaataggtg gttcagtatg ccaactgagt cttgaccaag cacttctgtt 180

accccggact gagtatcaat aagctgctca cgcggctgaa ggagaaaacg ttcgttatcc 240

ggccaattac ttcgagaaac ctagtaacac catgaaggtt gcgcagcgtt tcgctccgca 300

caaccccagt gtagatcagg tcgatgagtc accgcactcc ccacgggcga ccgtggcggt 360

ggctgcgttg gcggcctgcc catggggcaa cccatgggac gcttcaatac tgacatggtg 420

cgaagagtct attgagataa ttggtagtcc tccggcccct gaatgcggct aatcctaact 480

gcggagcaga tacccacatg ccagtgggca gtctgtcgta acgggcaact ctgcagcgga 540

accgactact ttgggtgtcc gtgtttcctt ttatttctat actggctgct tatggtgaca 600

attgacagat tgttaccata tagctattgg attggccatc cggtgacgaa cagagcaatt 660

atttacttat ttgttggttt tataccatta aatttcaaag ttttaaaaac cctcaactta 720

gttttgtcac tcaactcagc aaa 743

<210> 221

<211> 743

<212> DNA

<213> Enterovirus B88 (strain 11-4644-1)

<400> 221

tgtgctcttc cgatctggtt gttcccaccc acagggccca ctgggcgcca gcacactggt 60

attacggtac ccttgtgcgc ctgttttatc cacccccccc cactgtaact tagaagataa 120

ccacacgcgg tcaataggtg actcagtacg ccaactgggt cgcgaccaag cacttctgtt 180

accccggacc gagtatcaat aagctgctcg cgcggctgaa ggagaaaacg ttcgttaccc 240

ggccaattac ttcgagaaac ctagtaccac catggaggtt gcgcagcgtt tcgctccgca 300

caaccccagt gtagatcagg tcgatgagtc accgcaatcc ccacaggcga ctgtggcggt 360

ggctgcgttg gcggcctgcc catggggaaa cccatgggac gcttcaatat cgacatggtg 420

cgaagagtct attgagctaa ttggtagtcc tccggcccct gaatgcggct aatcctaact 480

gtggagcaga tacccacact ccagtgggca gtctgtcgta atgggcaact ctgcagcgga 540

accgactact ttgggtgtcc gtgtttcctt ttattcctat gctggctgct tatggtgaca 600

attgacagat tgttaccata tagctactgg attggccatc cggtgacaag cagagcgatt 660

gtttacttat ttgttggttt cattccactc aattataaag tactaaacac ccttaatttc 720

attataactc ttaacacgtt aaa 743

<210> 222

<211> 744

<212> DNA

<213> Human enterovirus B88 (strain BAN01-10398)

<400> 222

ttaaaacagc ctgtgggttg atcccaccca cagggcccac tgggcgctag cacactggta 60

ttccggtact tttgtgcgcc tgttttatac acccttcccc ttcatgtaac ttagaagtaa 120

aacacaaacg gtcaataggt agctcagcaa accagctgag cctcggccaa gcacttctgt 180

taccccggac tgagtatcaa taagctgtta acacggctga aggagaaaac gttcgttatc 240

cggccaatta cttcgagaaa cctagtaaca ccatgaaggt tgcgtagcgt ttcgctccac 300

acaaccccag tgtagatcag gtcgatgagt caccgctctc cccacgggtg accgtggcgg 360

tggctgcgtt ggcggcctgc ccatggagca ctccatggga cgcttcaata ctgacatggt 420

gtgaagagtc tattgagcta attggtagtc ctccggcccc tgaatgcggc taatcctaac 480

tgcggagcag acacccacac gccagtgggc agtctgtcgt aatgggcaac tctgcagcgg 540

aaccgactac tttgggtgtc cgtgtttcct tttattctta cattggctgc ttatggtgac 600

aatcaaggaa ttgttaccat atagctattg gattggccat ccggtatcta acagagcaat 660

tgtttacctc ttcaccggtt ttgtaccatt gaattgcaat agtctcaaca ccctttgcta 720

tatcattaaa ttgaactcag caaa 744

<210> 223

<211> 752

<212> DNA

<213> Enterovirus B93 (isolate 99052/XZ/CHN/1999)

<400> 223

aaagcaggct ttaaaacagc tctggggttg ttcccaccca cagggcccac tgggcgctag 60

cactctggta tcacggtacc tttgtgcgcc tgtttttcca cccctcccca tacgcaactt 120

agaagagaga ctcaaccggt caatagacag cctagtacac caactaggtt ctgaccaaga 180

acttctgttt ccccggaccg agtatcaata ggctgcttac gcggctgaag gagaaaacgt 240

tcgttacccg gccaattact tcgagaaacc tagtatcacc atggaagttg cgcagtgttt 300

cgctccgcac aaccccagtg tagatcaggt cgatgagtca ccgcattccc cacaggcgac 360

tgtggcggtg gctgcgttgg cggcctgccc gtggggcaac ccacgggacg cttcaatacc 420

gacatggtgc gaagagtcta ttgagctaat tggtagtcct ccggcccctg aatgcggcta 480

atcctaactg tggagcagat acccacgcgc cagtgggcag tctgtcgtaa tgggcaactc 540

cgcagcggaa ccgactactt tgggtgtccg tgtttccttt tatcctcata atggctgctt 600

atggtgacaa ttgaaagatt gttaccatat agctattgga ttggccatcc ggtgactaac 660

agagcaatta tttatttgtt tgttgggttt gtaccattga actacaaggt ccttaatact 720

ctaaactaca ttataagtct taatacagtg ca 752

<210> 224

<211> 744

<212> DNA

<213> Enterovirus B93 (isolate 38-03)

<400> 224

tttaaaacag cctgtgggtt gttcccrctc acagggccca ctgggcgcta gcacwctggt 60

atyacggtac cyttgtgcgc ctgttttacc acccctttcc ccaaagcaat ttagaagaaa 120

gactcaaccg gtcaatagac agcctagtac accaactagg ccctgaccaa gtacttctgt 180

ttccccggac tgagtatcaa taggctgctt acgcggccga aggagaaaac gttcgttacc 240

cggccaatta cttcgagaaa cccagtacca ccatgaaagt tgcgcagtgt ttcgctccgc 300

acaaccccag tgtagatcag gtcgatgagt caccgcatcc cccacaggtg actgtggcgg 360

tggctgcgtt ggcggcctgc ccatggggca acccatggga cgcttcaata ccgacatggt 420

gcgaagagtc tattgagcta attggtagtc ctccggcccc tgaatgcggc taatcctaac 480

tgtggagcag atacccacac accagtgggc agtctgtcgt aatgggcaac tccgcagcgg 540

aaccgactac tttgggtgtc cgtgtttcct tttattctca tattggctgc ttatggtgac 600

aattgaaagg ttgttgccat atagctattg gattggccac ccagtgacta acagagctat 660

tgtttatttg tttgttggat ttgtaccatt gaattacaag gtccttaata ctcttaacta 720

catcataaac ctcaatacga cgcg 744

<210> 225

<211> 742

<212> DNA

<213> Human enterovirus B97 (strain 99188/SD/CHN/1999/EV97)

<400> 225

ttaaaacagc ctgtgggttg ttcccaccca cagggcccac tgggcgctag cacactggta 60

tcacggtacc tttgtgcgcc tgttttatcc acccttcccc aagtgactta gaagcatacc 120

acacacggtc aacaggcggc tcaatgcacc aattgagtca tgaccaagta cttctgttac 180

cccggactta gtatcaataa gctgctcacg cggctgaagg agaaaacgtt cgttacccgg 240

ccaattactt cgagaaacct agtagcacca tggaggttgc gcggcgtttc gctccacaca 300

accccagtgt agatcaggct gatgagtcac cacaatcctc acgggcgacc gtggtggtgg 360

ctgcgctggc ggcctgccca tggggtaacc catgggacgc tacaatactg acatggtgtg 420

aagagtctat tgagctaatt ggtagtcctc cggcccctga atgcggctaa tcctaactgc 480

ggagcagata cccacacacc agtgggcagt ctgtcgtaac gggcaactct gcagcggaac 540

cgactacttt gggtgtccgt gtttcctttt ttctcttata ctggctgctt atggtgacaa 600

ttgaaagatt gttaccatat agctattgga ttggccatcc agtgacaaac agagcgattg 660

tttacctgtt cattggtttc gtaccgctga attacacagt tttaaacacc cttaatctta 720

ttttggcact taatacatca aa 742

<210> 226

<211> 742

<212> DNA

<213> Human enterovirus B97 (strain DT94-0227)

<400> 226

ttaaaacagc ctgtgggttg ttcccatcca cagggcccac tgggcgctag cacactggta 60

tcacggtacc tttgtgcgcc tgttttattt cccccctccc gaagtaacct agaagcaggt 120

aatatacggt caataggtga cacagtatac caactgggtc ttgaccaagc acttctgtta 180

ccccgggctg agtatcaata agctacttac gtggctgaag gagaaaacgc tcgttacccg 240

gccaattact tcgagaaacc tagtaacacc atgaaggttg cgcagtgttt cgctccgcac 300

aaccccagtg tagaccaggt cgatgagtca ccgcattccc cacgggcgac cgtggcggtg 360

gctgcgttgg cggcctgccc atggggcaac ccatgggacg cttcaaaact gacatggtgc 420

gaagagtcta ttgagctaat tggtagtcct ccggcccctg aatgcggcta atcccaactg 480

cggagcaaat acccacatgc cagtgggcag tctgtcgtaa tgggtaactc tgcagcggaa 540

ccgactactt tgggtgtccg tgtttctttt tattcttata ctggctgctt atggtgacaa 600

ttgaggaatt gttgccatat agctattgga ttggccatcc ggtgacaaac agagcaatta 660

tttacttgtt tgttggattc gtaccgctaa accacagagt actaagcacc cttaatttta 720

ttatattact taactcgaca aa 742

<210> 227

<211> 742

<212> DNA

<213> Human enterovirus B97 (strain BAN99-10355)

<400> 227

ttaaaacagc ctgtgggttg ttcccaccca cagggcccac tgggcgccag cacactggta 60

tcacggtacc tttgtgcgcc tgttttatcc acccttcccc aatgtaactt agaagtaaaa 120

ctagaaagat caatagcaag cacagtatgc caactgtgtt atgatcaagc acttctgtta 180

ccccggactg agtatcaata agctgctaaa gcggctgaag gagaaaacgt tcgttacccg 240

gccaactact tcgagaaacc cagtaccacc atgaaggttg cgcagtgttt cgctccacac 300

aatcccagtg tagatcaggt cgatgagtca ccgcgttccc cacgggcgac cgtggcggtg 360

gctgcgttgg cggcctgccc atgggatgac ccatgggacg cttcaatact gacatggtgc 420

gaagagtcta ttgagctagt tggtagtcct ccggcccctg aatgcggcta atcctaactg 480

cggagcagat acccacacac cagtgggcag tctgtcgtaa cgggtaactc tgcagcggaa 540

ccgactactt tgggtgtccg tgtttctttt tatcctcaaa ttggctgctt atggtgacaa 600

ttgataagtt gttaccatat agctattgga ttggccatcc ggtgtctaac agagctattg 660

tttatttatt tgttggtttc gtgccactaa attacaaagt ttttgaaact ctcaacttca 720

ttttgacact caataaagca aa 742

<210> 228

<211> 742

<212> DNA

<213> Human enterovirus B98 (strain: T92-1499)

<400> 228

ttaaaacagc ctgtgggttg ttcccaccca cagggcccaa tgggcgccag cactctggta 60

ttacggtacc tttgtgcgcc tgttttatat cccctccccc aattgtaact tagaagcaac 120

acgcaccgac caacagtggg cgtggcacac cagccacgtc ttggtcaagc acatctgttt 180

ccccggactg agtatcaata gactgctcac gcggtcgaag gagaaaacgt tcgttatccg 240

gccaactact tcgagaagcc cagtaacacc atgaatattg cgaagtgttt cgctcagcac 300

actcccagtg tagatcaggt cgatgagtca ccgcattccc cacgggcgac cgtggcggtg 360

gctgcgttgg cggcctgcct atggggcaac ccgtaggacg cttcaatatg gacatggtgt 420

gaagagtcta ttgagctagt tggtagtcct ccggcccctg aatgcggcta atcctaaccg 480

cggagcaggc acccacaaac cagtgggcag cttgtcgtaa cgggtaactc tgcggcggaa 540

ccgactactt tgggtgtccg tgtttccttt tattcttata ctggctgctt atggtgacaa 600

ttgagagatt gttgccatat agctattgga ttggccatcc ggtgacaaac agagcaattg 660

tttatctatt tgttggtttt ataccgttaa gttttaaagt cctaaaaacc cttaacttgg 720

tcatactact caactcagca aa 742

<210> 229

<211> 744

<212> DNA

<213> Human enterovirus B100 (isolate BAN2000-10500)

<400> 229

ttaaaacagt ctgtgggttg ttcccaccca cagggcccac gtggcgccag cacactggta 60

tctcggtacc tttgtgcgcc tgttttatcc accctaaacc ccgttgtaac ttagaagatg 120

agcaacactg atcaatagtg ggtgcaacat gccagttgca tcacgatcaa gcacttctgt 180

atccccggac tgagtatcaa taggctgctc acgcggctga aggagaaaac gttcgttatc 240

cggccagtta cttcgagaaa cctagtaaca tcatggaggt tgcgcagtgt ttcgctccgc 300

acacccccag tgtagatcag gtcgatgagt caccgcgttc cccacgggcg accgtggcgg 360

tggctgcgtt ggcggcctgc ccatgggata acccatggga cgcttcaata ctgacatggt 420

gtgaagagtc tattgagcta actggtagtc ctccggcccc tgaatgcggc taatcctaac 480

tgtggagcag acacccacat gccagtgggc agtctgtcgt aacgggcaac tccgcagcgg 540

aaccgactac tttgggtgtc cgtgtttcct tttattccta tattggctgc ttatggtgac 600

aattgaaaga ttgttaccat atagctattg gattggccat ccggtgtcaa acagagcaat 660

catttatttg tttgttggtt ttatacctct gaactttaaa gtgctaaaga ctctcaattt 720

tatattatat ctcaacaaat cagg 744

<210> 230

<211> 742

<212> DNA

<213> Human enterovirus B101 (strain CIV03-10361)

<400> 230

ttaaaacagc ctgtgggttg ttcccaccca cagggcccac tgggcgccag cactctggta 60

ttacggtacc tttgtgcgcc tgttttatat ccccctcccc acttgaaact tagaagtgaa 120

acacactgat caatagttga catggcaagc cagccatgtc ttgatcaagc acctctgttt 180

ccccggacct agtatcaata gactgctcgc gcggttgaaa gagaaagcgt tcgttacccg 240

gctaactact tcgagaaact tagtaacacc atggaagttg cggagtgttt cgctcagcac 300

atccccagtg tagatcaggc cgatgagtca ccgcattccc cacgggcgac cgtggcggtg 360

gctgcgttgg cggcctgcct gtggggcaac ccacaggacg ctctaataca aacatggtgc 420

gaagagtcta ttgagctagt tagtagtcct ccggcccctg aatgcggcta atcctaactg 480

cggagcacgt gcttccaatc caggaggtgg cgtgtcgtaa cgggtaactc tgcagcggaa 540

ccgactactt tgggtgtccg tgtttccttt tattcttgca ttggctgctt atggtgacaa 600

ttgagagatt gttaccatat agctattgga ttggccatcc ggtgacaaac agagcaatta 660

tatatctctt tgttggatac attccattga actacaagga gttaaaaacc ctcaattata 720

ttctgttctt gaattccgca ag 742

<210> 231

<211> 742

<212> DNA

<213> Enterovirus B106 (isolate AKS-AWT-AFP2F/XJ/CHN/2011)

<400> 231

ttaaaacagc ctgtgggttg ctcccaccca cagggcccaa tgggcgccag cacactagta 60

ttacggtact tttgtgcgcc tgttttatcc accctccccc cacgtaacat agaagcaaaa 120

cctacacggt caataggtgg ctcagtaagc caactgagcg gtgaccaagc acctctgtta 180

ccccggaccg agtatcaata ggctgctcac gcggctgaag gagaaaacgt tcgttacccg 240

gccaattact tcgagaaacc cagtatcacc atgaagattg cgaagtgttt cgctccgcac 300

aaccccagtg tagatcgggc tgatgagtca ccgcattcct cacgggcgac cgtggcggtg 360

gctgcgctgg cggcccgcct atggggcaac ccatgggacg cttcaatatt gacatggtgt 420

gaagagtcta ttgagctaat tggtagtcct ccggcccctg aatgcggcta atcctaactg 480

tggagcagat acccacgcac cagtgggcag tctgtcgtaa cgggcaactc tgcagcggaa 540

ccgactactt tgggtgtccg tgtttccttt tattcttata ctggctgctt atggtgacaa 600

ttgagagatt gttaccatat agctattgga ttggccatcc ggtgacaaac agagcaattg 660

tctacttatt cattggtttc gtaccactaa attacacagc attaaacact ctcaatttta 720

ttttaacact taatacatta aa 742

<210> 232

<211> 742

<212> DNA

<213> Human enterovirus 106 (isolate 148/YN/CHN/12)

<400> 232

ttaaaacagc ctgtgggttg ttcccaccca cagggcccat ggggcgccag cacactggta 60

ttgcggtacc tttgtgcgcc tgttttattc accctttccc aatgaaactt agaagcttaa 120

ctcaaccggt cgatagacag cccagtacac caactgggtt ttgaccaagt actcctgttt 180

ccccggactg agtatcaata agctgctcgc gcggctgaag gagaaaacgt tcgttacccg 240

gccaattact tcgagaaacc tagtaccacc atgaacgttg cgcagtgttt cgctccgcac 300

aaccccagtg tagatcaggt cgatgagtca ccgcaatccc cacgggcgac cgtggcggtg 360

gctgcgctgg cggcctgccc atggggcatc ccatgggacg cttcaatact gacatggtgc 420

gaagagtcta ttgagctaat tggtaatcct ccggcccctg aatgcggcta atcctaactg 480

cggggcagac acccacacgc cagtgggcag tctgtcgtaa cgggtaactc cgcagcggaa 540

ccgactactt tgggtgtccg tgtttccttt tatctttata ttggctgctt atggtgacaa 600

ttgaaagatt gttgccatat agctattgga ttggccatcc ggtgtctaat agagcaatta 660

tatatctctt tgttggcttc atcccgttaa ataaagtgag ctaccagacc ctgtgctata 720

ttatcaaatt gaacacaata aa 742

<210> 233

<211> 707

<212> DNA

<213> Enterovirus C96 (strain VEN/2018-23123A)

<400> 233

gcggccagta ctccggtatt acggtaccct tgtacgcctg ttttatactc ccttccccgt 60

aacttagaag acttcaaaga aatccaagtt caataggagg gggtacaaac cagtaccacc 120

acgaacaagc acttctgttc ccccggtgaa gttgtataga ctgtacccac ggttgaaaac 180

gactgatccg ttatccgctc taatacttcg agaagcttag tatcatcttg gaatcttcga 240

tgcgttgcgc tcagcactca accctggagt gtagcttagg tcgatgagtc tggacgttcc 300

tcaccggtga cggtggtcca ggctgcgttg gcggcctacc tgtggcccaa agccacagga 360

cgctagttgt gaacaaggtg tgaagagcct attgagctat tagagattcc tccggcccct 420

gaatgcggct aatcccaacc acggagcagg cgcttacaaa ccagtaagta gcttgtcgta 480

acgcgaaagt ctgtggcgga accgactact ttgggtgtcc gtgtttcctt ttatttttaa 540

atggctgctt atggtgacaa tcattgattg ttatcataaa gcgagttgga ttggccatcc 600

ggtgaaagtg agactcatca tatatctatt tgttggattt actcctttga ctaagtatac 660

tctcaacatt atattcattg taatattaat tagaagatac tatcata 707

<210> 234

<211> 747

<212> DNA

<213> Enterovirus C96 (isolate 127/SD/CHN/1991)

<400> 234

ttaaaacagc tctggggttg ttcccacccc agaggcccac gtggcggcca gtacaccggt 60

attacggtac ccttgtacgc ctgttttata ctccctaccc ctgcaactta gaagcaaata 120

atccaagttc aatagtaggg ggtacaaacc agtaccacca cgaacaagca cttctgtttc 180

cccggtgatg ttgtatagac tgtacccacg gttgaaaaca attgatccgt tacccgctct 240

tgtacttcga gaagcctagt atcatcttgg aaccttcgat gcgttgcgct cagcactcaa 300

ccccagagtg tagcttgggt cgatgagtct ggacaatcca caccggcgac ggtggtccag 360

gctgcgttgg cggcctacct gtggcccaaa gccacaggac gctagttgtg aacaaggtgt 420

gaagagccta ttgagctaca agagagtcct ccggcccctg aatgcggcta atcccaacca 480

cggagcaggt gatcacaacc cagtgattag cctgtcgtaa tgcgtaagtc tgtggcggaa 540

ccgactactt tgggtgaccg tgtttccttt tatttttatt gtggctgctt atggtgacaa 600

tcttagattg ttatcataaa gcgatttgga ttggccatcc ggtgaaagtt aaacacatta 660

tttacttgtt tgttggattc actccgttgt cctctcgcac tacgtatatt attaacgctg 720

tgctattaat taggtcaata catcaca 747

<210> 235

<211> 744

<212> DNA

<213> Enterovirus C96 (clone V13C)

<400> 235

ttaaaacagc tctggggttg ttcccacccc agaggcccac gtggcggcca gtacaccggt 60

attacggtac ccttgtacgc ctgttttata ctcccttccc cgcaacttag aagttaaaca 120

aaccaagttc aatagaaggg ggtgcaaacc agtaccacca cgaacaagca cttctgtttc 180

cccggtgacg tcatataggt tgtaaccacg gccaaaagtg actgatccgt tatccgctct 240

tgtacttcga gaagcccagt accatcttgg gatcttcgat gcgttgcgct cagcactcga 300

acccggagtg tagcttaggc cgatgagtct ggacattccc caccggtgac ggtggtccag 360

gctgcgttgg cggcctacct gtggtccaag ccacaggacg ctagatgtga acaaggtgtg 420

aagagcctat tgagctacaa gggaatcctc cggcccctga atgcggctaa tcctaactac 480

ggagcagacg gtcacaaacc agtgaatggt ctgtcgtaat gcgcaagtct gtagcggaac 540

cgactacttt gggtgtccgt gtttcctttt atctttttaa tggctgctta tggtgacaat 600

cctagattgt tatcataaag cgatttggat tggccatcca gtgatatttg aaaccattac 660

tgtattgcct gctaaggcta ttttaaagac ttaataaaca caacttcatt caactcaatt 720

acaccaacta acaactgtct cata 744

<210> 236

<211> 724

<212> DNA

<213> Enterovirus C99 (strain 10L1)

<400> 236

caccccagag gcccacgtgg cggctagtac tccggtatca cggtaccctt gtacgcctgt 60

tttatgctcc cttccccttg taacttagaa gcaaacaaca ctagttcaat aggagggggt 120

acaaaccagt accaccacga acacgcactt ctgtttcccc ggtgaagctg cataagctgt 180

tcccacggct gaaagcgact gatccgtcat ccgctcaggt acttcgggaa gcctagtacc 240

atcttggaat cttcgatgcg ttgcgctcag cactcaaccc cagagtgtag cttaggctga 300

tgagtctgga tgtgcctcac tggtgacagt ggtccaggct gcgttggcgg cctacccatg 360

gcccaaagcc atgggacgct agttgtgaac aaggtgtgaa gagcctactg agctacctga 420

gagtcctccg gcccctgaat gcggctaatc ccaaccacgg agcgagtgcc cacaatccag 480

tgagtggctc gtcgtaatgc gcaagtctgt ggcggaaccg actactttgg gtgtccgtgt 540

ttccttttaa ccttattatg gctgcttatg gtgacaatca tagattgtta tcataaagcg 600

aattggattg gccatccggt gaaagtcaga acgctaattt atctgtttgt tggatacacc 660

aaagttaact gcaatacact aaccttgatt cgcatagtgt tgttaattag atatcattac 720

caca 724

<210> 237

<211> 717

<212> DNA

<213> Human enterovirus C104 (isolate kvv585-16-TS)

<400> 237

ttaaaacagc ctgggggttg ttcccacccc cagggtccaa tggacgctag tacaccggta 60

tcacggtacc cttgtacgyc tgttttataa tccccttccc ccgtaacgtt agaagcacaa 120

aagcaaccgc tcaatagaca ccatacatcc agtgtggtta cgagcaagca cttctgtctc 180

cccggttgcg ccctatatgc tgtgtgaacg gcaaaaaggg gcaatatcgt tacccgcttg 240

actactacgg gaaacccagt atcatcttcg attgatttga gagttgcgtt cagcgcctga 300

cccccggcgt agctcaggct gatgaggctc cgaacacccc acaggtgact gtgtcggagc 360

ctgcgttggc ggcctgccca taccctgcgg tatgggacgc tcacttcatg acatggtgcg 420

aagagtctac tgtgctagtc aagattcctc cggcccctga atgtggataa tcctaaccac 480

tgatcctatg ttcgcaatcc agcgttcagt aggtcgtaac gcgcaagtcg gtggcggaac 540

caactacttt gggtgtccgt gtttccttta tttcctactg aatgcttatg gtgacaattg 600

ttttgatcag ttgttgccat tgctcgcatt cagtgtgcaa ttacaaaaac aaaactatca 660

gttgttacca tccctcacgt tcagtgtata atcacaaaaa cgagtttttc tgttata 717

<210> 238

<211> 635

<212> DNA

<213> Human enterovirus C105 (strain USA/OK/2014-19362)

<400> 238

cccctaggat ccactggatg ttagtacact ggtatcgcgg tacctttgta cgcctgtttt 60

atatcccctt ccccgcaact ttagaagtat caaaagtacc gctcaatagc cgccacgctc 120

ccagtgtggt ttcgagcaag cacttctgtt ttcccggttg cgtcccatat gctgtgcaaa 180

cggcaaaaag ggacaatatc gttacccgct tgtatactac gggaaaccta gtaccgccat 240

tgattgtatt gagggttgcg ctcatcacct ttcccggtgt agctcaggct gatgaggctc 300

agaatccccc acaggtaact gtgtctgagc ctgcgttggc ggcctgccct cgccttatgg 360

cgtgggacgc tcactacatg acatggtgcg aagagtctac tgtgctatac aagagtcctc 420

cggcccctga atgtggataa tcctaaccac tgatcccacg tacgcaaacc agcgtgtagt 480

gggtcgtaac gcgcaagtcg gtggcggaac cgactacttt gggtgaccgt gtttccttta 540

ttacctattg aatgtttatg gtgacaattg tttgattcag ttgttaccat ttgctacatt 600

caataaccta gtgtcaaacc gattgaattg ctaca 635

<210> 239

<211> 689

<212> DNA

<213> Human enterovirus C116 (strain 126)

<400> 239

ttcccactcc agaggcccac gtggcggcta gtactctggt attgcggtac ctttgtacgc 60

ctgttttata ccccctcccc aagtaacttt agaagcaatt cacaaagttc aatagagggg 120

gtacaaacca gtaccaccac gaacaagcac ttctgtttcc ccggtgaagc tacatagact 180

gtccccacgg tcgaaggtgg cagatccgtt atccgctcaa gtacttcgag aagcctagta 240

ccaccttgga atcttcgatg cgttgcgctc agcactctac cccgagtgta gcttaggtcg 300

atgagtctgg gcatgcccca ccggtgacgg tggcccaggc tgcgttggcg gcctacccgt 360

ggctaacgcc atgggacgct agttgtgaac aaggtgtgaa gagcctattg agctacttga 420

gagtcctccg gcccctgaat gcggctaatc ctaaccacgg agcaagtgct cacaaaccag 480

tgagtggctt gtcgtaacgc gtaagtctgt ggcggaaccg actactttgg gtgtccgtgt 540

ttccttttat tttcattatg gctgcttatg gtgacaatct aagattgtta tcatatagct 600

tttggattgg ccatccggtg atttgaaaga gatatattta ccttattgtt ctaccatatc 660

taattactca tcacagccac ataatcaca 689

<210> 240

<211> 672

<212> DNA

<213> Enterovirus C117 (strain JX-C117-40-2017)

<400> 240

tttaaaacag cttgagggtt gttcccaccc tcagggccta ctgggcgcta gtacaccggt 60

atctcggtac ccttgtacgc ctgttttatc ttccctcccc aaaagtaact ttagaagcaa 120

gtaaactcac agctcaacag ccatcgcacc gccagtgcga ttttgagcaa gcactcctgt 180

ttccccggtt gcgtcccata tgctgtgaaa acggcagaag gggacaatat cgttacccgc 240

ttatctacta cgggaagcct agtaacacca ttgagcactt tgagggttgc gttcagcgct 300

aaacccagcg tagctcaggc tgatggggct tcggaatccc cacgggtgac cgtgtcgaag 360

cccgcgttgg cggcctgccc acaccaatgg gtgtgggacg ctcaatttgt gacatggtgc 420

gaagagccta ctgtgctagg tgagagtcct ccggcccctg aatgtggcta atcctaacca 480

ccgaacccgt ggttgcaatc cagcaactag cgggccgtaa tgcgcaagtc ggtgatggaa 540

ccaactactt tgggtgaccg tgtttccttt atctcttata ttgcaatttt atggtgacat 600

ttatagtatt taagtgttac catatattgc gcaaatacat tgtaataata cagagttatt 660

actacggaaa tc 672

<210> 241

<211> 669

<212> DNA

<213> Human enterovirus C118 (isolate CQ5185)

<400> 241

tttttaaaac agcctggggg ttgtttcccc ctccgaggtc ccctggacgc ccgtaccccg 60

gcatcacggt gcccttgtat gcctgtttta taatcccccc cccccgcaac tttagaagaa 120

aacgaactcc gagctcagta ggcgtcacgt ttccagcgtg actacgagca agcacttctg 180

tttccccggt tgcgccctat atgctgtgtg aacggcagaa aggggcaaaa tcgttatccg 240

cttgattact gcgggacgcc tagtatcact atggattgac ttgagggttg cgctcagcac 300

atccccagtg tagcttgggc cgatgaggct ccgaattccc cacgggcgac cgtgtcggag 360

cctgcgttgg cggcctaccc gcgcctcacg gcgtgggacg ctcatttcat gacatggtgc 420

gaagagtcta ctgcgctaat caaggctcct ccggcccctg aatgtggcta atcctaacca 480

ctgatcctgt gagcacacgc cagtgcccag caggtcgtaa tgcgcaagtc ggtggcggaa 540

ccaactactt tgggtgtccg tgtttccttt atttcctcat tgaatgctta tggtgacaat 600

tgttgagtac agttgttacc attactgcac tctgcaaata gtgaataaac cacgcaacaa 660

tataacaca 669

<210> 242

<211> 732

<212> DNA

<213> Human enterovirus D68 (strain Fermon)

<400> 242

ttaaaacagc tctggggttg ttcccactca agggcccacg tggcggctag tactctggta 60

tctcggtacc tttgtacgcc tgttttaatt ccctccccaa cgtaacttag aagcttttaa 120

accaaagctc aataggtgga gcgcaaacca gcgctcttat gagcaagcac ttctgtctcc 180

ccggtgtggt tgtatagact gtccccacgg ttgaaaacaa cttatccgtt aaccgctata 240

gtacttcgag aaacctagta ttgccttcgg agtgttgatg cgttgcgctc agcacactaa 300

cccgtgtgta gcttgggtcg atgagtctgg acgtacccca ctggcgacag tggtccaggc 360

tgcgttggcg gcctactcat ggtgaaaacc atgagacgct agacatgaac aaggtgtgaa 420

gagtctattg agctgctata gagtcctccg gcccctgaat gcggctaatc ctaaccatgg 480

agcaagtgct cacaaaccag tgagttactt gtcgtaacgc gcaagtccgt ggcggaaccg 540

actactttgg gtgtccgtgt ttcacttttt acttttatga ctgctaatgg tgacaattta 600

atattgttac catttggctt gtcgaattga tcacataaga tctatagttt tgttcactga 660

tttgctttga aataatctca cctcaaaacc tccagtacat aacatttaaa gagtttaaac 720

ttatttataa ca 732

<210> 243

<211> 710

<212> DNA

<213> Enterovirus D68 (TBp-13-Ph209)

<400> 243

ttaaaacagc cttggggttg ttcccacccc aagggcccac gtggcggcta gtactctggt 60

atttcggtac ctttgtacgc ctgttttatc tccctcccta atgtaactta gaagctttca 120

aatcaaagct caataggtgg agcgcaagcc agcgcttccg tgagcaagca ctcctgtttc 180

cccggtgcgg ttgtatagac tgttcccacg gttgaaaaca acctatccgt tatccgctat 240

agtacttcga gaaacctagt atcacctttg gattgttgat gcgttgcgct cagcacacta 300

acccgtgtgt agcttgggtc gatgagtctg gacatacccc actggtgaca gtggtccagg 360

ctgcgttggc ggcctactca tggtgaaaac catgagacgc taaacatgaa caaggtgtga 420

agagtctatt gagctactat agagtcctcc ggcccctgaa tgcggctaat cttaaccatg 480

gagcaagtgc ttacaaacca gtgagttgct tgtcgtaatg cgcaagtccg tggcggaacc 540

gactactttg ggtgtccgtg tttcactttt tacttttatg actgcttatg gtgacaattt 600

aatattgtta ccatttagct agtcaaatta atcatataaa actctaaatc ccatctaaca 660

attcacattt ggatagcttc attttgagga ttttaaacct atttacaaag 710

<210> 244

<211> 726

<212> DNA

<213> Enterovirus D70 (strain JPN/1989-23292)

<400> 244

ttaaaacagc tctggggttg ttcccacccc agaggcccac gtggcggcta gtactccggt 60

actccggtac ccttgtacgc ctgttttata ctcccctccc caagcaactt tagaagatat 120

aaactaatgt tcaacaggag ggggtacaaa ccagtaccac cacgaacaga cacttctgtt 180

tccccggtga agttgcatag actgtaccca cggttgaaag cgatgaatcc gttatccgct 240

taggtacttc gagaagccta gtatcgtctt ggaatcttcg atgcgttgcg ctcagcactc 300

taccccgagt gtagcttagg tcgatgagcc tggacacccc acaccggcga cggtggtcca 360

ggctgcgttg gcggcctacc catggctaac accatgggac gctagttgtg aacaaggtgt 420

gaagagccta ttgagctacc taagagtcct ccggcccctg aatgcggcta atcccaacca 480

cggagcaaat gctcacaatc cagtgagtgg tttgtcgtaa cgcgcaagtc tgtggcggaa 540

ccgactactt tgggtgtccg tgtttccttt tatttttatt atggctgctt atggtgacaa 600

tctgagattg ttattatata gctattggat tggccatccg gtgatatctt gaaattttgc 660

tataattttc cacaaatttt acaacatcac actacacctt cttttaaaca attgagacaa 720

ctcata 726

<210> 245

<211> 753

<212> DNA

<213> Enterovirus D94 (strain ANG/2010-23293)

<400> 245

ttaaaacagc tctggggttg ttcccacccc agaggcccac gtggcggcta gtactccggt 60

attacggtac ccttgtacgc ctgttttata ctcccttccc ccgtaactta gaagtaacac 120

aaatccaagt tcaatagaag ggggtacaaa ccagtaccac catgaacaag cacttctgtt 180

tccccggtga aatcatagac tgtacccacg gttgaaagtg atcgatccgt catccgctct 240

tgtacttcga gaagcctagt atcaccttgg aatcttcgat gcgttgcgct caacactcaa 300

ccccagagtg tagcttaggc tgatgagtct ggacactccc caccggcgac ggtggtccag 360

gctgcgttgg cggcctacct gtggtccaaa gccacaggac gctagttgtg aacaaggtgt 420

gaagagccta ttgagctaca ggagagtcct ccggcccctg aatgcggcta atcccaacca 480

cggagcaggt ggtcataatc caatgattgg cctgtcgtaa cgcgcaagtc tgtggcggaa 540

ccgactactt tgggtgtccg tgtttccttt tattttatca ttggctgctt atggtgacaa 600

tctaagattg ccatcatata gcttttggat tggccatccg gtgatacttc gagttattta 660

gcttgctgct tacatttatt ttaaacaaca tccagttacc agcattccta ttttgttttt 720

ctttcattct gagttatcta ataagtagtt aga 753

<210> 246

<211> 681

<212> DNA

<213> Human enterovirus D94 (isolate 19/04)

<400> 246

ttggtacctt tgggggcctg ttttatatcc cttccctcat tgtaacttag aagtttcaaa 60

atacagctca atagacagta gtgcaaacca gtactgctac gagcaagtac ttctgttacc 120

ccggtgaagt tgcatagact gtacccacgg ttgaaagtga ctgatccgtt atccgcttta 180

gtacttcgag aagcctagta acatcttgga atcttcgatg cgttgcgctc agcactcaac 240

cccagagtgt agcttaggtc gatgagtctg gacattcctc accggcgacg gtggtccagg 300

ctgcgttggc ggcctacctg tggcccaaag ccacaggacg ctagttgtga acaaggtgtg 360

aagagcctat tgagctacta aagagtcctc cggcccctga atgcggctaa tcccaaccac 420

ggagcaagtg ctcacaatcc agtgagtggc ttgtcgtaac gagcaattct gtggcggaac 480

cgactacttt gggtgtccgt gtttcctttt atttttatca ttggctgctt atggtgacaa 540

tctaagattg ccatcatata gcttttggat tggccatccg gtgatatttc gagctataca 600

acttactgtt tacagctctt ttaaatagca ttcaattacc agcactcata ctttgttttg 660

aattactacc aaatagtcag a 681

<210> 247

<211> 742

<212> DNA

<213> Enterovirus D111 (strain ANG/2010-23294)

<400> 247

ttaaaacagc tctggggttg ttcccacccc agaggcccac gtggcggcca gtactccggt 60

attgcggtac ccttgtacgc ctgttttata ctcccttccc ccgtaactta gaagaaataa 120

accaagttca ctaggagggg gtacaaacca gtaccaccac gaacaagcac ttctgtttcc 180

ccggtgacat tgcatagact gtacccacgg ttgaaagcga ctgatccgtt acccgctact 240

gtacttcgag aagcctagta ccatcttgga atcttcgacg cgttgcgctc agcactcaac 300

cccagagtgt agcttaggtc gatgagtctg gacaatcccc accggtgacg gtggtccagg 360

ctgcgttggc ggcctacctg tggcccaaag ccacaggacg ctagttgtga acaaggtgtg 420

aagagcctat tgagcttcag tagagtcctc cggcccctga atgcggctaa tcccaaccac 480

ggagcaggtg gtcataatcc aatgactggc ctgtcgtaac gcgcaagtct gtggcggaac 540

cgactacttt gggtgtccgt gtttcctttt attttgtcaa tggctgctta tggtgacaat 600

ctaagattgc catcatatag cttttggatt ggccatccgg tattattttg agttatttga 660

tttgttgtcc acagctcttt acagtatcac ttaactatta ttgtttgtat tttgctcctt 720

ggatcacaac caggtaatca ga 742

<210> 248

<211> 709

<212> DNA

<213> Enterovirus D111 (isolate D111-NGR-KAT-1263)

<400> 248

aggcccacgt ggcggccagt actctggtat tacggtacct ttgtacgcct gttttatatc 60

ccttccccca atgtaactta gaagttctta aacatagctc aatagacagt agtacacacc 120

agtactgcta cgagcaagca cttctgttac cccggtgaag ttacatagac tgtacccacg 180

gttgaaagcg actgatccgt tatccgcttt agtacttcga gaagcctagt aatatcttgg 240

aatcttcgat gcgttgcgct caacactcaa ccccagagtg tagcttaggt cgatgagtct 300

ggacattccc caccggcgac ggtggtccag gctgcgttgg cggcctacct gtgacccaaa 360

gtcacaggac gctagttgtg aacaaggtgt gaagagccta ttgagctact aaagagtcct 420

ccggcccctg aatgcggcta atcccaacca cggagcaagt gctcacaacc cagtgagtgg 480

cttgtcgtaa cgagtaattc tgtggcggaa ccgactactt tgggtgtccg tgtttccttt 540

atttttatca ttggctgctt atggtgacaa tctaagattg ccatcatata gcttttggat 600

tggccatccg gtgatatttt gagttattca aattgctatt tacagcattc tttgtacact 660

gttcattcat cactatttgc attttgttct tgaattttaa acagtcaaa 709

<210> 249

<211> 654

<212> DNA

<213> Simian enterovirus J103 (isolate cg8227)

<400> 249

tttgtacgcc tattttaaaa cccctacccc cgtaacttag aagcatttga cacaagttca 60

atagtaggac catcatccag gtggttcatc gaacaagcac ttctgttacc ccggtcctag 120

tacctgtgaa gctgtaccca cggcggaagg ggaaaacgat cgttacccgg ctatctactt 180

cgagaagcct agtaacagca ttgaaactct tgagcgttgc gttcagcaca gtaacccctg 240

tgtagctttg gctgatgagt ctgggcattc cccactggcg acagtggccc aggctgcgtt 300

ggcggccaac cgatccgggc aaccgggtcg gacgcttatt tgtggacatg gtgtgaagag 360

cctattgagc tagatagtag tcctccggcc cctgaatgcg gctaatccta actccggagc 420

aaatgcacgc aacccagcgt gtagtttgtc gtaacgcgta agtctggagc ggaaccgact 480

actttgggtg tccgtgtttc ctgttttacc ttatttggct gcttatggtg acaatatagt 540

attgttacca tatagctatt ggattggcca tccggtgaac agatttcgac aaactctatt 600

ttactgttgt tcacagccta aagttacaaa tcttactctg actcagtagt aatc 654

<210> 250

<211> 680

<212> DNA

<213> Coxsackievirus A2 (isolate HN202009)

<400> 250

cgcgtcttgt gcgctgtttt ataggcctcc tccttaatag gttacttaga agctgacatt 60

tacacgacca atagtgggcg tggcgcacca gccacgtctt ggtcaagcac tcctgtttcc 120

ccggaccgag tatcaatagg ctgctcacgt ggctgaaaga gaaagtgttc gttatccggc 180

taactacttc gggaaaccca gtaacaccat gaaagttgca gagtgtttcg ctcagcactt 240

cccccgtgta gatcaggctg atgagtcact gcgaacccca cgggcgaccg tggcagtggc 300

tgcgttggcg gcctgcctat ggggtaaccc ataggacgct ctaatacaga catggtgcga 360

agagtctact gagctagtta gtattcctcc ggcccctgaa tgcggctaat cctaactgcg 420

gagcacatac cctcaatcca gagggcggtg tgtcgtaacg ggcaactctg cagcggaacc 480

gactactttg ggtgtccgtg tttcctttta tccttatgct ggctgcttat ggtgacaatt 540

gaaaggttgt taccatatag ctattggatt ggccatccgg tgactaacag agctttggtt 600

tatcaattca ttgggttcgt gcccttatca tctacagttt atacaacttt ggtatacttg 660

ttatatctta atagtaagaa 680

<210> 251

<211> 746

<212> DNA

<213> Coxsackievirus A2 (isolate 16027)

<400> 251

ttaaaacagc ctgtgggttg tacccaccca cagggcccac tgggcgttag cacactgatt 60

ctacggaacc tttgtgcgcc tgttttatat ccctccccta aaatagtaac ttagaagttg 120

aacatttaca cgaccaatag tgggcgtggc gcaccagcca cgtcttggtc aagcactcct 180

gtttccccgg actgagtatc aataggctgc tcacgtggct gaaggagaaa gtgttcgtta 240

tccggctaac tacttcggga aacccagtaa caccatgaaa gttgcagagt gtttcgctca 300

gcacttcccc cgtgtagatc aggctgatga gtcactgcga accccacggg cgatcgtggc 360

agtggctgcg ttggcggcct gcctatgggg caacccatag gacgctctaa tacagacatg 420

gtgcgaagag tctactgagc tagttggtat tcctccggcc cctgaatgcg gctaatccta 480

actgcggagc acatgccctc aatccagagg gtggtgtgtc gtaacgggca actctgcagc 540

ggaaccgact actttgggtg tccgtgtttc cttttactct tatactggct gcttatggtg 600

acaattgaaa ggttgttacc atatagctat tggattggcc atccggtgac taacagagct 660

ttgatttatc aattcattgg gtttgtgccc ttatcatcta cagtttactc aaccttggtg 720

tacttgttat atcttaatag taagaa 746

<210> 252

<211> 746

<212> DNA

<213> Coxsackievirus A2 (isolate CVA2-1388-M14/XY/CHN/2017)

<400> 252

ttaaaacagc ctgtgggttg tacccaccca cagggcccac tgggcgttag cacactgatt 60

ctacggaatc tttgtgcgcc tgttttatat ccctccccta aaatagtaat ttagacgttg 120

aacatttaca cgaccaatag tgggcgtggc gcaccagtca cgtcttggtc aagcactcct 180

gttcccccgg accgagtatc aataggctgc tcacgtggct gaaggagaaa gtgttcgtta 240

tccggctaac tacttcggga aacccagtaa caccatgaaa gttgcagagt gtttcgttca 300

gcacttcccc cgtgtagatc aggctgatga gtcactgcaa accccacggg cgaccgtggc 360

agtggctgcg ttggcggcct gcctatgggg taacccatag gacgctctaa tacagacatg 420

gtgcgaagag tctactgagc tagttagtat tcctccggcc cctgaatgcg gctaatccta 480

actgcggagc acatgccctc aatccagagg gtggtgtgtc gtaatgggca actctgcagc 540

ggaaccgact actttgggtg tccgtgtttc cttttattct tatactggct gcttatggtg 600

acaattgaaa agttgttacc atatagctat tggattggcc atccggtgaa taacagagct 660

ttggtttatc agttcattgg gttcgtgccc ttatcatcta cagcttacac aactttggtg 720

tacttgttat atcttaatag taagaa 746

<210> 253

<211> 746

<212> DNA

<213> Coxsackievirus A2 (isolate CVA2/Shenzhen50/CHN/2012)

<400> 253

ttaaaacagc ctgtgggttg tacccaccca cagggcccac tgggcgttag cacactgatt 60

ctacggaatc tttgtgcgcc tgttttatat ccctccccta aaatggtaac ttagaagttg 120

aacatttaca cgaccaatag tgggcgcggc gcaccagcca cgtcttggtc aagcactcct 180

gtttccccgg actgagtatc aataggctgc tcacgtggct gaaggagaaa gtgttcgtta 240

tccggctaac tacttcggga aacccagtaa caccatgaaa gttgcagagt gtttcgctca 300

gcacttcccc cgtgtagatc aggctgatga gtcactgcga accccacggg cgaccgtggc 360

agtggctgcg ttggcggcct gcctatgggg caacccatag gacgctctaa tacagacatg 420

gtgcgaagag tctactgagc tagttagtat tcctccggcc cctgaatgcg gctaatccta 480

actgcggagc acatgccctc aatccagagg gtggtgtgtc gtaacgggca actctgcagc 540

ggaaccgact actttgggtg tccgtgtttc cttttattcc tgtactggct gcttatggtg 600

acaattgaaa ggttgttacc atatagctat tggattggcc atccggtgac taacagagct 660

ttggtttatc aattcattgg gttcgtaccc ttatcatcta cagtttattc aactttggtt 720

tacttgttat atcttaatag caagaa 746

<210> 254

<211> 746

<212> DNA

<213> Coxsackievirus A2 (strain 2260165)

<400> 254

ttaaaacagc ctgtgggttg tacccaccca cagggcccac tgggcgttag cacactgatt 60

ctacggaatc tttgtgcgcc tgttttatat ccctccccta aaatagtaac ttagaagttg 120

aacatctaca cgaccaatag tgggcgtggc gcaccagcca cgtcttggtc aagcactcct 180

gtttccccgg actgagtatc aataggctgc tcacgtggct gaaggagaaa gtgttcgtta 240

tccggctaac tacttcggga aacccagtaa caccatgaaa gttgcagagt gtttcgctca 300

gcacttcccc cgtgtagatc aggctgatga gtcactgcga accccacggg cgaccgtggc 360

agtggctgcg ttggcggcct gcctatgggg caacccatag gacgctctaa tacagacatg 420

gtgcgaagag tctactgagc tagttggtat tcctccggcc cctgaatgcg gctaatccta 480

actgcggagc acatgccctc aatccagagg gtggtgtgtc gtaacgggca actctgcagc 540

ggaaccgact actttgggtg tccgtgtttc cttttactct tatactggct gcttatggtg 600

acaattgaaa ggttgttacc atatagctat tggattggcc atccggtgac taacagagct 660

ttgatttatc aattcattgg gtttgtgccc ttatcatcta cagtttattc aaccttggtg 720

tacttgttat atcttaatag taagaa 746

<210> 255

<211> 744

<212> DNA

<213> Coxsackievirus A4 (strain CA4/JX2204/2014)

<400> 255

cagtgggccc tgtgggttgt acccacccac agggcccact gggcgctagc actctgattc 60

cacgggatcc ttgtgcgcct gttttatatc ccctccccaa gttgtaactt agaagtatta 120

cactacaatg atcagtagca ggcgtggcgc accagccatg tctcgatcaa gcacttctgt 180

ttccccggac cgagtatcaa tagactgcta acgcggttga aggagaaaac gttcgttatc 240

cggctaacta cttcgagaaa cctagtagca ccatcgaagc tgcggggcgt ttcgctcagc 300

acttcccccg tgtagatcag gtcgatgagt cactgcaatc ctcacgggcg accgtggcag 360

tggctgcgtt ggcggcctgc ctatggggca acccatagga cgcttcaaag tggacatggt 420

gtgaagagtc tattgagcta gttagtagtc ctccggcccc tgaatgcggc taatcctaac 480

tgcggagcac ataccttcaa tccagaaggt agtgtgtcgt aacgggcaac tctgcagcgg 540

aaccgactac tttgggtgtc cgtgtttcct tttattctct tattggctgc ttatggtgac 600

aattgaagaa ttgttaccat atagctattg gattggccat ccagtgtcta acagagcaat 660

tgtgtacttg tttgttggtt ttgttccctt gtcttacacg tctcttagca ccttgtatta 720

cattataaaa ctgaactcgg aaag 744

<210> 256

<211> 748

<212> DNA

<213> Coxsackievirus A4 (isolate HK458564/2016)

<400> 256

tttaaaacca agctgtgggt tgtacccact cacagggccc actgggcgct agcactctga 60

ttccgcggaa tctttgtgcg cctgttttat aacccctccc ccgatttgta atttagaagt 120

aatgcacacc actgatcaac agcaagcgtg gcgcgccagc catgttcaga tcaaatactt 180

ctgttttccc ggactgagta tcaatagact gctcacgcgg ttgaaggaga aaacgttcgt 240

tacccggcta actacttcga gaaacctagt agcaccatcg aaactgcaga gtgtttcgct 300

cagcactttc cccgtgtaga tcaggtcgat gagtcactgc aatccccacg ggcgaccgtg 360

gcagtggctg cgttggcggc ctgcctatgg gcttgcccat aggacgctct aatgtggaca 420

tggtgtgaag agtctattga gctagttggt agtcctccgg cccctgaatg cggctaatcc 480

taactgcaga gcgcataccc tcaatccagg gggtggtgcg tcgtaacggg caactctgca 540

gcggaaccga ctactttggg tgtccgtgtt tccttttatt cttatattgg ctgcttatgg 600

tgacaattga gagattgttg ccatatagct attggattgg ccatccagtg tctaacagag 660

caattgtata cttgtttgtt ggattcgttc cactgtcata tacatccact aacaccttat 720

actatattac aaaattgaac acaagaaa 748

<210> 257

<211> 745

<212> DNA

<213> Coxsackievirus A5 (isolate CV-A5-3487-M14-XY-CHN-2017)

<400> 257

ttaaaacagc ctgtgggttg tacccacccg cagggcccac tgggcgctag cactctggta 60

ccacggtacc tttgtgcgcc tgttttatct ccccaaaccc cgtaacgcta cattagaagt 120

taaacaatat gaccgatagt cagtggggca acccagcctc actttggtca agcacttctg 180

ttctcccggg cttagtacca ataagctgct cgcgcggctg aaggggaacg cgcccgttat 240

ccggctaact acttcgagaa acctagtacc accatggaag ttgcagagtg tttcgctcag 300

cactacccca gtgtagatca ggctgatgag tcaccgtgca ccccacgggc gaccgtgacg 360

gtgactgcgt tggcggcctg cccatggtgt ataccatggg acgctctaat acagacatgg 420

tgcgaagagt ctattgagct agttagtagt cctccggccc ctgaatgcgg ctaatcctaa 480

ctgcggagca cgcgctctca acccagagag tagcgtgtcg taacgggtaa ctctgcagcg 540

gaaccgacta ctttgggtgt ccgtgtttct ttttattctt acattggctg cttatggtga 600

caattgagag attgttacca tatagctatt ggattggcca tccggtgtgt aacagagcta 660

tcatatattc ttttgttggc ttcgtgccac tagattatac tgcaattgtt actttaaggt 720

acatcttact tctgaatacg gtaaa 745

<210> 258

<211> 746

<212> DNA

<213> Coxsackievirus A5 (strain CVA5/13164/HUN/2015)

<400> 258

tttaaaacag cctgtgggtt gtacccaccc acagggccca ctgggcgcta gcactctggt 60

actacggtac ccttgtgcgc ctgttttatc ttcccaaacc ccgtaacgct acactagaag 120

ttagacactg tgaccaatag tcagtggggc aacccagccc cacttcggtc aagtacctct 180

gtttccccgg gtctagtacc aataggctgc ttacgcggct gaaggggagc gcgcccgtta 240

tccggctaac tacttcgaga aacctagtac caccatggaa gttgcagagt gtttcgctca 300

gcacaacccc agtgtagatc aggtcgatga gtcaccgtac accccacggg cgaccgtgac 360

ggtggctgcg ttggcggcct gcccatggtg cacaccatgg gacgctctaa tacagacatg 420

gtgcgaagag tctattgagc tagttagtag tcctccggcc cctgaatgcg gctaatccta 480

actgcggagc atgtgctctc aacccagagg gtagcatgtc gtaacgggca actctgcagc 540

ggaaccgact actttgggtg tccgtgtttc cttttattct tatattggct gcttatggtg 600

acaattgaaa gattgttacc atatagctat tggattggcc atccggtgtg caatagagct 660

attgtatatt cttttgttgg ctttgtgcca ttagattata cggcaactgg tactttaaga 720

tacattcagc ttttgaacac agcaaa 746

<210> 259

<211> 741

<212> DNA

<213> Coxsackievirus A6 (isolate DN1501)

<400> 259

gacttgtggg ttgtacccac ccacagggcc cactgggcgc tagcacactg attctacgga 60

atctttgtgc gcctgtttta taaccccttc ccccaaaact gtaacttaga agaatattac 120

actattgatc aatagcaggc atggcgcgcc agtcatgtct agatcaagca cttctgtctc 180

cccggactga gtatcaatag actgctagcg cggttgaagg agaaaacgtc cgttacccgg 240

ctaactactt cgagaaactt agtagcacca ttgaagctgc ggagtgtttc gttcagcact 300

cccccagtgt agatcaggtc gatgagtcac tgcactcccc acgggcgacc gtggcagtgg 360

ctgcgttggc ggcctgccta tggggcaacc cataggacgc tctaaagtgg acatggtgtg 420

aagagtctat tgagctagtt agtagtcctc cggcccctga atgcggctaa tcccaactgc 480

ggagcacatg ctctcaatcc agagggtggt gtgtcgtaac gggcaactct gcagcggaac 540

cgactacttt gggtgtccgt gtttcctttt attcttatat tggctgctta tggtgacaat 600

tgagagattg ttaccataca gctattggat tggccatcca gtgacaagca gagccttgat 660

atacttgttc gtgggttttg ttccactcac cagtcgtaca gttcatactc taaagtacat 720

tctgattctg aacaatagaa a 741

<210> 260

<211> 748

<212> DNA

<213> Coxsackievirus A6 (strain RYN-A1205)

<400> 260

ttaaaacagc cctgtgggtt gtacccaccc acagggccca ctgggcgcta gcacactgat 60

tctatggaat ctttgtgcgc ctgttttata accccttccc caaaactgta acttagaaga 120

atatcacact accgatcaat agcaggcatg gcgcgccagt catgtctaga tcaagcactt 180

ctgtctcccc ggattgagta tcaatagact gctagcgcgg ttgaaggaga aaacgtccgt 240

tacccggcta actacttcga gaaacttagt agcaccattg aagctgcgga gtgtttcgct 300

cagcactctc ccagtgtaga tcaggtcgat gagtcactgc actccccacg ggcgaccgtg 360

gcagtggctg cgttggcggc ctgcctatgg ggcaacccat aggacgctct aaagtggaca 420

tggtgcgaag agtctattga gctagttagt agtcctccgg cccctgaatg cggctaatcc 480

taactgcgga gcacatgccc tcaatccagg gggtggtgtg tcgtaacggg caactctgca 540

gcggaaccga ctactttggg tgtccgtgtt tccttttttt cttatattgg ctgcttatgg 600

tgacaattga gagattgtta ccatatagct attggattgg ccatccagtg acaaacagag 660

ctttgatata cttgttcgtg ggtttcgttc cactcatcag tcgtacagtt catactttaa 720

agtacattct gattctgaac aatagaaa 748

<210> 261

<211> 746

<212> DNA

<213> Coxsackievirus A7 (strain MAD-3101-11)

<400> 261

tttaaaacag cctgtgggtt gcacccaccc acagggccca ctgggcgcca gcactctgat 60

tccgcggaat ctttgtgtgc ctgttttatc acccttcccc caaacttgca acttagaagc 120

agcacttcac tgaccaatag caggcgtggc gcaccagcca tgtctaggtc aagcacttct 180

gtttccccgg actgagtatc aataggctgc tcacgcggct gaaggagaaa acgttcgtca 240

tccggctaac tacttcgaga aacctagtag caccggtgaa gctgcagagt gtttcgctca 300

gcacttcccc cgtgtagatc aggtcgatga gtcactgcac tccccacggg cgaccgtggc 360

agtggctgcg ctggcggcct gcctatgggt caacccatag gacgctctaa tgtggacacg 420

gtgcgaagag tctattgagc tagttagtag tcctccggcc cctgaatgcg gctaatccta 480

actgcggagc acatgccccc gaaccagggg gtggtgtgtc gtaacgggca actctgcagc 540

ggaaccgact actttgggtg accgtgtttc cttttgttct tattatggct gcttatggtg 600

acaattgaag aattgttacc atatagctat tggattggcc atccagtgtc taacagagct 660

atcatatacc tatttgttgg attcgttcta ttgtcataca catcactcaa tacattacat 720

tttattgtga gattaaatac aagaaa 746

<210> 262

<211> 748

<212> DNA

<213> Coxsackievirus A8 (isolate 13-467/GS/CHN/2013)

<400> 262

ttaaaacagc ctgtgggttg cacccaccca cagggcccac tgggcgctag cactctgatt 60

ctatggaatc tttgtgcgcc tgttttataa ccccctcccc caagtttgta atttagaagc 120

aaagtaccgt aacgatcaac agcaggcgtg gcgtaccagc catgtctcga tcaagcactt 180

ctgtttcccc ggacagagta tcaatagact actcacgtgg ttgaaggaga aaacgtccgt 240

tatccggcta actacttcga gaaacctagt aacaccattg aagctgcaga gcgtttcgct 300

cagcacttac cccgtgtaga tcaggtcgat gagtcactgc attccccacg ggcgaccgtg 360

gcagtggctg cgttggcggc ctgcctatgg ggtcacccat aggacgctct aatgtggaca 420

tggtgcgaag agtctattga gctagttagt agtcctccgg cccctgaatg cggctaatcc 480

taactgcgga gcgcataccc tcaaaccagg gggtggtgcg tcgtaacggg taactctgca 540

gcggaaccga ctactttggg tgtccgtgtt tccttttatt ttcttactgg ctgcttatgg 600

tgacaattga agaattgttg ccatatagct attggattgg ccatccggtg tgtaacagag 660

caatcatcta tttgtttgtc gggtatatcc cactaacgct caactctttt aacacattac 720

agtatatact aatcctgaat acaagaaa 748

<210> 263

<211> 721

<212> DNA

<213> Coxsackievirus A8 (isolate C177/CHW/AUS/2017)

<400> 263

ccactcacag ggcccactgg gcgctagcac tctggtaata cgttaccttt gtgcgcctgt 60

tttatctccc cctaccctgg aacaccttag aagcaaagca tctctgatca atagaaggtg 120

cagcgaacca gctgcgtctc gatcaagcac ttctgtttcc ccggactgag tatcaataga 180

ctgctaacgc ggtcgaagga gaaaacgttc gttatccggc tagctacttc gagaagccta 240

gtaacaccat gaaagttgca gagtgtttca ctcagcactt cccccgtgta gatcaggtcg 300

atgagtcacc gcgatcccca cgggcgaccg tggcggtggc tgcgttggcg gcctgcctat 360

ggggcaaccc ataggacgct ctaatacaga catggtgtga agagcctatt gagctagcta 420

gtagtcctcc ggcccctgaa tgcggctaat cctaactgcg gagcacatgc tcgtgaacca 480

gcgagtagtg tgtcgtaatg ggcaactctg cagcggaacc gactactttg ggtgtccgtg 540

tttcttttta ttttctctat tggctgctta tggtgacaat taaagaattg ttaccatata 600

gctattggat tggccatccg gtgactaata gagctattat atatctcttt gttggatttg 660

tccccttgag gctcactgct tataacacac tttgttatat attgactctc aactcagcaa 720

a 721

<210> 264

<211> 747

<212> DNA

<213> Coxsackievirus A8 (isolate CV-A8/P82/2013/China)

<400> 264

ttaaaacagc ctgtgggttg ttcccactca cagggcccac tgggcgctag cacactgatt 60

ctccggtacc tttgtgtgct tgttttagta tccctccccc aaactcgtaa cttagaagaa 120

taacacgcta tcgatcaata gcaggcatgg cgcaccagcc atgtctcgat caagcacttc 180

tgtttccccg gacagagtat caatagacta ctcacgtggt tgaaggagaa aacgtccgtt 240

atccggctaa ctacttcgag aaacctagta acaccattga agctgcagag cgtttcgttc 300

agcacttacc ccgtgtagat caggtcgatg agtcactgca ttccccacgg gcgaccgtgg 360

cagtggctgc gttggcggcc tgcctatggg gtcacccata ggacgctcta atgtggacat 420

ggtgcgaaga gtctattgag ctagttagta gtcctccggc ccctgaatgc ggctaatcct 480

aactgcggag cgcataccct caaaccaggg ggtagtgcgt cgtaacgggc aactctgcag 540

cggaaccgac tactttgggt gtccgtgttt ccttttattt tcttactggc tgcttatggt 600

gacaattgaa gaattgttgc catatagcta ttggattggc catccggtgt gtaacagagc 660

aatcatctat ttgtttgccg ggtacatccc attaacgctc aactctttta gtacattaca 720

gtatatacta atcctgaata caagaaa 747

<210> 265

<211> 743

<212> DNA

<213> Human coxsackievirus A8 (strain Donovan)

<400> 265

ttaaaacagc ctgtgggttg cacccaccca cagggcccaa tgggcgtcag cactctggta 60

ccgaggtacc tttgtgcgcc tgttttattt ccccttcccc tgatgcaact tagaagctcc 120

gaactaatga tcaatagtag gtgtggcacg ccagccacat cttgatcaag cacttctgtt 180

tacccggacc gagtatcaat aagctgcgca agcggctgaa ggagaaagcg ttcgttatcc 240

ggccaactac ttcgagaagc ttagtaccac catgaacgtt gcagagtgtt tcgttcagca 300

caaccccggt gtagatcagg ccgatgagtc accgcgttcc ccatgggcga ccatggcggt 360

ggctgcgttg gcggcctgcc catggagcaa tccatgggac gctctaatac tgacatggtg 420

cgaagagcct attgagctag ttggtgatcc tccggcccct gaatgcggct aatcctaact 480

gcggagcatg cgcccacaag ccagtgggtg gtgtgtcgta acgggtaact ctgcagcgga 540

accgactact ttgggtgtcc gtgtttcctt ttatctttac attggctgct tatggtgaca 600

attgaagaat tgttaccata tagctattgg attggccatc cggtgtgcaa tagagcgatt 660

atatacctat ttgttggatt tgttccattg acatatagat ctcttaacac tctacaacac 720

atcttgatct tgaacacgag aaa 743

<210> 266

<211> 726

<212> DNA

<213> Coxsackievirus A10 (isolate TA111R)

<400> 266

gtgggggtat cacagggccc actgggcgct agcactctga ttctgcggaa tctttgtgcg 60

cctgttttat aacctccccg aaacttgcaa cttagaagtt atgtacgtta ctgatcaata 120

gcaggcgtgg cacaccagcc atgtcttgat caagcacttc tgtacccccg gactgagtat 180

caatagactg ctcacgtggt cgaaggagaa aacgttcgtt atccggctaa ctacttcgag 240

aaacctagta acaccactga aactgcggag tgtttcgctc agcacttccc ccgtgtagat 300

caggtcgatg agccactgct taccccacgg gtgaccgtgg cagtggctgc gttggcggcc 360

tgcctatggg gcaacccata ggacgctcta aagtggacat ggtgcgaaga gtctattgag 420

ctagttagta gtcctccggc ccctgaatgc ggctaatcct aactgcggag cgcatgcccc 480

caaaccagag ggtggtgcgt cgtaacgggt aactctgcag cggaaccgac tactttgggt 540

gtccgtgttt cttttattct cataatggct gcttatggtg acaattgagg aattgttacc 600

atatagctat tggattggcc atccggtgtg caacagagct attatctact tgtttgttgg 660

atacattcca ttaacaccca aatccttcaa tacattgcat tacattctaa cgctgaacgc 720

aagaaa 726

<210> 267

<211> 747

<212> DNA

<213> Coxsackievirus A10 (strain CA10/JX2545/2017)

<400> 267

tttaaaacag cctgtgggtt gtacccaccc acagggccca ctgggcgcta gcactccgat 60

tctgcggaat ctttgtgcgc ctgttttata accctccccc gaaacttgta atttagaagt 120

tatgtacgtt actgatcaac agcaggcgtg gcacaccagc catgtcttga tcaagcactt 180

ctgtaacccc ggactgagta tcaatagact gctcacgtgg tcgaaagaga aaacgttcgt 240

tacccggcta actacttcga gaaacctagt agcaccactg aagctgcgga gtgtttcgct 300

caacacttcc cccgtgtaga tcaggtcgat gagtcactgc ttaccccacg ggtgaccgtg 360

gcagtggctg cgttggcggc ctgcctatgg ggcaacccat aggacgctct aaagtggaca 420

tggtgtgaag agtctattga gctagttagt agtcctccgg cccctgaatg cggctaatcc 480

taactgcgga gcgcatgccc ccaaaccaga gggtggtgcg tcgtaacggg taactctgca 540

gcggaaccga ctactttggg tgtccgtgtt tcttttattc ttataatggc tgcttatggt 600

gacaattgag gaattgttac catatagcta ttggattggc catccggtgt gcaacagagc 660

tattatctac ttgtttgttg gtttcattcc attgacacct aaatccttca atacattgca 720

ctatattcta acgttgaacg caagaaa 747

<210> 268

<211> 740

<212> DNA

<213> Coxsackievirus A12 (isolate D89)

<400> 268

ttaaaacagc ctgtgggttg cacccactca cagggcccac tgggcgtcag cactctggta 60

cctcggtacc tttgtgcgcc tgttttatat ccccccccca ttgaaactta gaagcaatgc 120

acttcgatca atagcgggcg cggcgcacca gccacgtcat gatcaagcac ttctgtttcc 180

ccggaccgag tatcaataga ctgctaacgc ggttgaggga gaaaacgtcc gttacccggc 240

caactgcttc gagaaatcca gtaacatcat gaaaattgca gagtgtttcg ctcagcactt 300

ccccagtgta gatcaggtcg atgagtcacc gcgttcctca cgggcgaccg tggcggtggc 360

tgcgttggcg gcctgcctgt ggggcaaccc gcaggacgct ctaatatgga catggtgtga 420

agaatctatt gagccagttg gtagtcctcc ggcccctgaa tgcggctaat cctaactgcg 480

gagcacgcgc cctcaatcca gtgggtagcg tgtcgtaatg ggtaactctg cagcggaacc 540

gactactttg ggtgtccgtg tttcctttta ttcttacatt ggctgcttat ggtgacaatt 600

gagagattgt taccatatag ctattggatt ggccatccgg tatcgaatag agcggttatc 660

tatcaattta ttggatttac tccgttgagt gtgcagcagt atcgcacttt gcttttaata 720

gtgagactga atagtgcaaa 740

<210> 269

<211> 739

<212> DNA

<213> Coxsackievirus A12 (strain QD-LXH535/SD/CHN/2009)

<400> 269

ttaaaacagc ctgtgggttg cacccactca cagggcccac tgggcgtcag cactctggta 60

cctcggtacc tttgtgcgcc tgttttatat ccccccccat tgaaacttag aagcaatgca 120

cctcgatcaa tagcgggcgt ggcgcaccag ccacgtcatg atcaagcact tctgtttccc 180

cggaccgagt atcaatagac tgctaacgcg gttgagggag aaaacgtccg ttacccggcc 240

aactgcttcg agaaatccag taacatcatg aaaattgcag agtgtttcgc tcagcacttc 300

cccagtgtag atcaggtcga tgagtcaccg cgttccccac gggcgaccgt ggcggtggct 360

gcgttggcgg cctgcctgtg gagcaaccca caggacgctc taatatagac atggtgtgaa 420

gaatctattg agccagttgg tagtcctccg gcccctgaat gcggctaatc ctaactgcgg 480

agcacgtgcc ctcaatccag tgggtagcgt gtcgtaatgg gtaactctgc agcggaaccg 540

actactttgg gtgtccgtgt ttccatttat tcttacattg gctgcttatg gtgacaattg 600

aaagattgtt gccatatagc tattggattg gccatccggt atcgaataga gcggttatct 660

atcagtttat tggatttatc ccgttgagtg tgcagcagta tcgcactttg cttttaatag 720

tgagactgaa tagtataaa 739

<210> 270

<211> 750

<212> DNA

<213> Coxsackievirus A14 (strain MAD-72-07)

<400> 270

ttaaaacagc tctggggttg tacccactca cagggcccac tgggcgctag cactctgatt 60

ctacggaatc cttgtgcgcc tgttttataa ccccctccca atatgtaact tagaagcagc 120

acatactacc gatcaatagt gggcatggcg caccagccat gtctcgatca agcacttctg 180

tttccccgga ctaagtatca atagactgct cacgcggttg aaggagaaag cgtccgttac 240

ccggctaact acttcgagaa acccagtagc accactgaag ctgcagagtg tttcgctcag 300

cacttccccc gtgtagatca ggtcgatgag tcaccgcaat ccccacgggc gaccgtggcg 360

gtggctgcgt ggcggcctgc ctgtggggca acccacagga cgctctaaag tggacatggt 420

gcgaagagtc tattgagcta gttagtagtc ctccggcccc tgaatgcggc taatcctaac 480

tgcggagcgc atgccttcaa accagatggt agtgcgtcgt aatgggcaac tctgcagcgg 540

aaccgactac tttgggtgtc cgtgtttcct tttattctta cattggctgc ttatggtgac 600

aatcacagaa ttgttaccat atagctattg gattggccat ccggtgtgta atagagcaat 660

aatttactct tttgttgctt ttgttccatt gaccttcact acactgaaca ctttacagta 720

catacttcaa ctcaactcca gaaagcaatc 750

<210> 271

<211> 708

<212> DNA

<213> Coxsackievirus A14 (isolate SEN-14-254)

<400> 271

ggggccggag ctctggtgat acgttacctt tgtgcgcctg ttttatttcc ctaccccgga 60

acaatctaga agcaacgcgc tactggccaa tagaaagtgt agcaaaccag ctacatcccg 120

gtcaagcact tctgtctccc cggaccgagt atcaataaac tgctaacgcg gttgaaggag 180

aaaaggttcg ttacccggcc aactacttcg aaaagcccag taacaccatg aaagttgcag 240

agtgtttcgc tcagcacttc ccccgtgtag atcaggtcga tgagtcaccg cgatccccac 300

gggcgaccgt ggcggtggct gcgttggcgg cctgcctatg gggcaaccca taggacgctc 360

taatacagac atggtgcgaa gagcctattg agctagttgg tagtcctccg gcccctgaat 420

gcggctaatc ctaactgcgg agcttatgct cgcgaaccag cgagtagtga gtcgtaacgg 480

gtaactctgc agcggaaccg actactttgg gtgtccgtgt ttccttttat tctcatattg 540

gctgcttatg gtgacaatca aagaattgtt accatatagc tattggattg gccatccggt 600

gtgcaacaga gctattgtct actcttttgt tggatttgtt ccattgtcct ctttcaccac 660

actaaatact ctacagtata tactttccct taattcaagg aaacgatc 708

<210> 272

<211> 755

<212> DNA

<213> Human coxsackievirus A14 (strain G-14)

<400> 272

ttaaaacagc ctgtgggttg tacccaccca cagggcccac tgggcgctag cacactgatt 60

ctgcgggatc tttgtgcgcc tgttttatat cccctccccc caaactaggt aacttagaag 120

attagcactt gtatgaccaa tagtaggcgt gacgcgccag tcatgtcttg gtcaagcact 180

tctgtttccc cggactgagt atcaataagc tgcttgcgcg gctgaaggag aaaacgttcg 240

ttacccggct aactacttcg agaaacctag taacaccatg aaagttgcgg agtgtttcgc 300

tcagcacttc ccccgtgtag atcaggtcga tgagtcactg caaaccccac gggtgaccgt 360

ggcagtggct gcgttggcgg cctgcctatg ggtcaaccca taggacgctc taatacagac 420

atggtgtgaa gagtctattg agctagttag tagtcctccg gcccctgaat gcggctaatc 480

ctaactgcgg agcacacgct tgcaaaccag tgagtagtgt gtcgtaatgg gcaactctgc 540

agcggaaccg actactttgg gtgtccgtgt ttccttttat tctcatactg gctgcttatg 600

gtgacaattg aagaattgtt actatatagc tattggattg gccatccggt gtgtaacaga 660

gcgataatct actcctttgt tgcatttgtt ccactaaccc ttactacatt agacattcta 720

caatacatac tttatctcaa ctccaagaaa cgaac 755

<210> 273

<211> 744

<212> DNA

<213> Coxsackievirus A16 (isolate AH17-18/AH/East/CHN/2017-02-12)

<400> 273

ttaaaacagc tctggggttg tccccaccca cagggcccac tgggcgctag cacactggtt 60

ctgcgggacc tttgtgcgcc tgttttataa cccttcccta agcagtaact tagaagcttt 120

gtacaatcat ggccaatagt gggcgtggcg cgccagtcac gtcttggtca agcacttctg 180

tatccccgga ctgagtatca atagactgct cacgcggttg aaggagaaaa cgttcgttac 240

ccggctaact acttcgagaa acctagtagc accatgaaag ttgcggagtg tttcgctcag 300

cacttccccc gtgtagatca ggtcgatgag ccactgtaaa ccccacgggc gaccgtgaca 360

gtggctgcgt tggcggcctg cccatggggt aacccatggg acgctctaat acagacatgg 420

tgcgaagagt ctattgagct agttagcagt cctccggccc ctgaatgcgg ctaatcctaa 480

ctgcggagcg cgcaccttca acccaggggg cggcgcgtcg taatgggtaa ctctgcagcg 540

gaaccgacta ctttgggtgt ccgtgtttcc ttttattctt tattggctgc ttatggtgac 600

aattgaaaag ttgttaccat atagctattg gattggccat ccggtgtcta acagagctat 660

tgtttaccta ttcattggct ttgtccctct taatctcaag gccatccaaa ctctcgatta 720

catattgctc ctcagctgta agaa 744

<210> 274

<211> 730

<212> DNA

<213> Coxsackievirus A16 (isolate CV-A16/HVN08.039_HA_GIANGVNM/2008)

<400> 274

ggttgttccc acccacaggg cccactgggc gctagcacac tgattctacg ggatctttgt 60

gcgcctgttt tataaccctt cccctaagca gcaacttaga agctttgtac aatcacgacc 120

agtagtgggc gtggcgcgcc agtcacgtct tggtcaagca cttctgtatc cccggactga 180

gtatcaatag actgctcacg cggttgaagg agaaaacgtt cgttacccgg ctaactactt 240

cgagaaacct agtagcaccg tgaaagttgc ggagtgtttc gctcagcact tcccccgtgt 300

agatcaggtc gatgagtcac tgtaaacccc acgggcgacc gtgacagtgg ctgcgttggc 360

ggcctgccca tggggtaacc catgggacgc tctaatacag acatggtgtg aagagtctat 420

tgagctagtt agtagtcctc cggcccctga atgcggctaa tcctaactgc ggagcgcgca 480

ccctcaaccc agggggcggc gtgtcgtaat gggtaactct gcagcggaac cgactacttt 540

gggtgtccgt gtttcctttt attccttatt ggctgcttat ggtgacaatt gaaaagttgt 600

taccatatag ctattggatt ggccatccgg tgtctaatag agctattgtt tacctattta 660

ttggatacgt ccctcttaat ctcaaagcca ttcaaactct tgattatata ttgctcctta 720

actgtaagag 730

<210> 275

<211> 742

<212> DNA

<213> Coxsackievirus B1 (strain RO-98-1-74)

<400> 275

ttaaaacagc ctgggggttg ttcccaccca cagggcccac tgggcgctgg cacactggta 60

ttacggtacc tttgtgcgcc tgttttattt acccttcccc aatgcaactt agaagtataa 120

ctcaaacggt caacagataa ctcagtatgc cagctgagcc tagaccaagc acttctgtta 180

ccccggaccg agtatcaata agctgctcgc gcggctgaag gagaaaaagt tcgttacccg 240

gccaattact tcgagaaact tagtaccacc atggaggttg cgcagtgttt cgctccacac 300

acccccagtg tagatcaggc cgatgagtca ccgcgttccc cacgggcgac cgtggcggtg 360

gctgcgttgg cggcctgccc atggggcaac ccatgggacg cttcaatact gacatggtgc 420

gaagagtcta ttgagctaat tggtagtcct ccggcccctg aatgcggcta atcctaactg 480

cggagcagat acccacgcgc cagtgggcag tctgtcgtaa tgggcaactc tgcagcggaa 540

ccgactactt tgggtgtccg tgtttccttt tattcttgta ttggctgctt atggtgacaa 600

ttgagagatt gttaccatat agctattgga ttggccatcc ggtgacaaac agggcgatca 660

tctatttgtt tgttggtttt ataccattga actacaaagt cctagatact cttaacctta 720

tattaaaatt taacacatta aa 742

<210> 276

<211> 734

<212> DNA

<213> Coxsackievirus B1 (strain CVB1/XM0108)

<400> 276

tcctgtgggt tgatcccacc cacagggccc actgggcgct agcacactgg tattccggta 60

cctttgtgcg cctgttttat acacccttcc caagtgtaac ttagaagctt atcacacacg 120

gtcaacaggt gactcagtat gccaactggg tcttgatcaa gcacttctgt ttccccggac 180

tgagtatcaa taagctgctc acgcggctga aggagaaaac gttcgttaac cggccaatta 240

cttcgagaaa cctagtaaca ccatggaggt tgcgcagtgt ttcgctccac acaaccccag 300

tgtagatcag gccgatgagt caccgcactc ctcacgggcg accgtggcgg tggctgcgct 360

ggcggcctgc ccatgggata acccatggga cgcttcaata ctgacatggt gcgaagagtc 420

tattgagcta attggtagtc ctccggcccc tgaatgcggc taatcctaac tgcggagcag 480

atacccacac gccagtgggc agtctgtcgt aatgggcaac tctgcagcgg aaccgactac 540

tttgggtgtc cgtgtttcct tttattttta tactggctgc ttatggtgac aattgagaga 600

ttgttaccat atagctattg gattggccat ccagtgacaa acagagcgat tatttattta 660

tttgttggtt atatcccatt aagccaaaag gccctagtta ctctcaactt tatactattg 720

cttaatacaa cgaa 734

<210> 277

<211> 741

<212> DNA

<213> Coxsackievirus B1 (strain B1/Groningen/2011)

<400> 277

taaaacagcc tgtgggttgt tcccacccac agggcccatt gggcgctagc acactgatat 60

cacggtacct ttgtgcgcct gttttatcta cccctccccg atgcaactta gaagcatgtt 120

acaaacggtc aataggtggt tcagttaacc aactgagcct cgaccaagca cttctgttac 180

cccggactga gtatcaatag gctgctcgcg tggctgatgg agaaaacgtc cgttacccgg 240

ccaattactt cgagaaacct agtaccacca tggaagttgc gcagcgtttc gctccgcaca 300

accccagtgt agatcaggtc gatgagtcac tgcgttcccc acgggtgacc gtggcagtgg 360

ctgcgttggc ggcctgccca tggggtatcc catgggacgc ttcaatactg acatggtgtg 420

aagagtctat tgagctaatt ggtagtcctc cggcccctga atgcggctaa tcctaactgc 480

ggagcagata cccacgcacc agtgggcagt ctgtcgtaac gggcaactct gcagcggaac 540

cgactacttt gggtgaccgt gtttcctttt attttaatat tggctgctta tggtgacaat 600

tgaaagattg ttgccatata gctattggat tggccatccg gtaacaaaca gagcaattat 660

ttacctgttt attggtttta tacctctcaa ccacaaggtt ataagtactc tcaaccttgt 720

tttagcattt aacacagcaa a 741

<210> 278

<211> 735

<212> DNA

<213> Coxsackievirus B2 (strain 13-2380-2_B2)

<400> 278

agcctgtggg ttgcacccac ccacagggcc cactgggcgc cagcactctg gtactgcggt 60

acctttgtgc gcctgtttta tcaccccacc ccatccggaa cgtagaagct agacttaaat 120

ggtcaataga cggcccagta tgccaattgg gtctcgacca agcactcctg tttccccggg 180

tttagtatca ataagctgct cacgtggctg aaggagaaaa cacccgttat ccggccaact 240

acttcgagaa acttagtacc gctatgaacg ttgcaaggcg tttcgctcag cacttccccc 300

gtgtagatca ggccgatgag tcaccgcatt cctcacgggc gaccgtggcg gtggctgcgc 360

tggcggcctg cctatgggct tgcccatagg acgccttaat actgacatgg tgcgaagagt 420

ctattgagct agttggtagt cctccggccc ctgaatgcgg ctaatcctaa ctgcggagca 480

catactcaca aaccagtgag cagtgtgtcg taacgggcaa ctctgcagcg gaaccgacta 540

ctttgggtgt ccgtgtttcc ttttattcct acactggctg cttatggtga caattaaaga 600

attgttacca tatagctatt ggattggcca tccggtgtct aacagagcta ttatatactt 660

gtttgttgga ttcataccac ttgattccaa tagctataac accctacttt acatcgttca 720

tttgaacacg agaaa 735

<210> 279

<211> 720

<212> DNA

<213> Coxsackievirus B2 (strain 14L)

<400> 279

cccacccaca gggcccactg ggcgtcagca cactggtatt gcggtaccct tgtgcgcctg 60

ttttatccac cccacccaaa agcaacttag aagcacgtca tatacggtca ataggcggcc 120

cagcactcca actgggtctc gaccaagcac ttctgttacc ccggaccgag tatcaatagg 180

ctgctcacgc ggctgaggga gaaagcgttc gttacccgac caattacttc gagaaaccta 240

gtaccaccat gaaggttgcg cagcgtttcg ttccgcacaa ccccagtgta gatcaggccg 300

atgagtcacc gcattcccca cgggcgaccg tggcggtggc tgcgttggcg gcctgcccat 360

ggggcaaccc atgggacgct tcgatactga catggtgtga agagtctatt gagctaattg 420

gtagtcctcc ggcccctgaa tgcggctaat cctaactgcg gagcagatac ccacaaacca 480

gtgggcggtc tgtcgtaacg ggcaactctg cagcggaacc gactactttg ggtgtccgtg 540

tttcctttta ttcttgtatt ggctgcttat ggtgacaatt gagagattgt taccatatag 600

ctattggatt ggccatccgg tgacaaatag agcaatcatc tatctattcg ttggttttgt 660

accgctaacc tttaaagttt taaaaacttt aaatttcatt ttagccctaa acatagcaaa 720

<210> 280

<211> 742

<212> DNA

<213> Coxsackievirus B2 (strain 08-749-Shimane08-JPN)

<400> 280

ataaaacagc ctgtgggttg ttcccaccca cagggcccac tgggcgtttg cacactggta 60

tcgcggtacc tttgtgcgcc tgttttatcc accctcccct aattgaattt agaagtcaag 120

cacaaccggt caacagatag cccagcatac cagctgggtt tcgaccaagc acttctgttt 180

acccgggctg agtatcaata ggctgctcac gcggctgaag gagaaaacgt tcgttacccg 240

gccaattact tcgagaaacc tagtaccgcc atggacgttg cgcagcgttt cgttccacac 300

aaccccagtg tagatcaggt cgatgagtca ccgcatcccc cacgggtgac cgtggcggtg 360

gctgcgctgg cggcctgccc atggggtaac ccatgggacg cttcaatgct gacatggtgc 420

gaagagtcta ttgagctaat tggtagtcct ccggcccctg aatgcggcta atcctaactg 480

cggagcagac acccacacgc cagtgggcag tctgtcgtaa cgggcaactc cgcagcggaa 540

ccgactactt tgggtgtccg tgtttccttt tattctttca ttggctgctt atggtgacaa 600

ttgagaggtt gttaccatat agctattgga ttggccatcc ggtgagcaac agagcaatta 660

tatttctttt tgttggatat ataccacttg actctaataa ctacaatacc ctgctttata 720

ttctccaatt gaatacgaaa ag 742

<210> 281

<211> 742

<212> DNA

<213> Coxsackievirus B2 (strain RW41-2/YN/CHN/2012)

<400> 281

ttaaaacacc ctgtgggttg ttcccaccca cagggcccat agggcgctag cacactggta 60

ttgcggtacc tttgtgcgcc tgttttattc atccttcccc aatggaactt agaagcttaa 120

ctcaaccggt caatagacag cccagtacac caactgggtt acgaccaggc actcctgttt 180

ccccggactg agtatcaata agctgctcac gcggctgaag gagaaaacgt tcgttacccg 240

gccaattact tcgagaaacc tagtaccgcc atggacgttg cgcagtgttt cgctccgcac 300

aaccccagtg tagatcaggt cgatgagtca ccgcgatcct cacgggcgac cgtggcggtg 360

gctgcgctgg cggcctgccc atggggcaac ccatgggacg cttcaatact gacatggtgt 420

gaagagtcta tcgagctaat tggtagtcct ccggcccctg aatgcggcta atcctaactg 480

cggggcagat acccacacgc cagtgggcag tctgtcgtaa cgggcaactc cgcagcggaa 540

ccgactactt tgggtgtccg tgtttccttt tattcttata ttggctgctt atggtgacaa 600

ttgaaagatt gttgccatat agctattgga ttggccatcc agtcagtaat agagcaatta 660

tatttctttt tgttgggttt gtaccacttg atattaacaa ttataacacc ttactataca 720

tcgtccattt gaacacaaga aa 742

<210> 282

<211> 688

<212> DNA

<213> Coxsackievirus B2 (isolate BCH314)

<400> 282

tggtattgcg gtacctttgt gcgcctgttt tattcaccct tccccaatga aacttagaag 60

tttaactcaa ccggtcgata gacagcccag tacaccaact gggttttgac caagtactcc 120

tgttttcccc ggactgagta tcaataagct gctcgcgcgg ctgaaggaga aaacgttcgt 180

tacccggcca attacttcga gaaacctagt accaccatgg acgttgcgca gtgtttcgct 240

ccgcacaacc ccagtgtaga tcaggtcgat gagtcaccgc aatccccacg ggcgaccgtg 300

gcggtggctg cgctggcggc ctgcccatgg ggcaacccat gggacgcttc aatactgaca 360

tggtgcgaag agtctattga gctaattggt agtcctccgg cccctgaatg cggctaatcc 420

taactgcggg gcagataccc acacgccagt gggcagtctg tcgtaacggg taactccgca 480

gcggaaccga ctactttggg tgtccgtgtt tccttttatt cttatattgg ctgcttatgg 540

tgacaattga aagattgttg ccatatagct attggattgg ccatccagtc agcaatagag 600

caattatatt tctttttgtt gggtttgtac cacttgataa taacaactac aacaccttac 660

tatacattat ccatttgaac acgagaaa 688

<210> 283

<211> 728

<212> DNA

<213> Coxsackievirus B3 (isolate B307)

<400> 283

gagggttcca tgcccacagg gcccactggg cgctagcaca ctggtatcgt ggtacctttg 60

tgcgcctgtt ttatccaccc tgaccccaag gcaacgtaga agcataccac taaaggtcaa 120

taggcgatcc agcactccag ctgggttaag accaagcact tctgttaccc cggaccgagt 180

accaataagc tgctcacgcg gctgaagggg aaagcgttcg ttacccaacc aattacttcg 240

agaaacctag taccaccgtg aaagttgcgc agcgtttcgc tcagcacaac cccagtgtag 300

atcaggttga tgagtcaccg cattccccac gggcgaccgt ggcggtggct gcgttggcgg 360

cctgcccatg gggcaaccca tgggacgctt caatactgac atggtgtgaa gagtctattg 420

agctaattgg taatcctccg gcccctgaat gcggctaatc ctaactgcgg agcagatacc 480

cacacaccag tgggcagtct gtcgtaatgg gtaactctgc agcggaaccg actactttgg 540

gtgtccgtgt ttccttttaa cttatactgg ctgcttatgg tgacaattga tagattgtta 600

ccatatagct attggattgg ccatccagtg acaagcagag ctatcattta cctgtttgtt 660

ggttttgtcc cattgaattt taaggttcta aaaactattg actttatctt agcactcaat 720

acggcaaa 728

<210> 284

<211> 742

<212> DNA

<213> Coxsackievirus B3 (isolate 2001-5)

<400> 284

ttaaaacagc ctgtgggttg ttcccaccca cagggcccac tgggcgctag cacactggta 60

ttgcggtacc tttgtgcgcc tgttttatcc accctcccca accgtaattt agaagcacga 120

acaatgtggt caacaggcaa cccagtgcac caactgggtc ttgaccaagc acttctgtta 180

cccccggact gagtatcaat aagctgctcg cgtggctgaa agagaaaacg ttcgttaccc 240

ggccaattac ttcgagaaac ctagtatcac catggaagtt gcgcagtgtt tcgctccgca 300

caaccccagt gtagatcagg tcgatgagtc accgcaaccc tcacgggtga ccgtggcggt 360

ggctgcgctg gcggcctgcc cgtggggcaa cccatgggac gcttcaatac tgacatggtg 420

tgaagagtct attgagctaa ttggtagtcc tccggcccct gaatgcggct aatcctaact 480

gcggagcaga tatccacaca ccagtggaca gtctgtcgta atgggcaact ctgcagcgga 540

accgactact ttgggtgtcc gtgtttcctt ttatctttta ttggctgctt atggtgacaa 600

ttgagagatt gttaccatat agctattgga ttggccatcc ggtgagcaac agagcagttg 660

tattcctctt tgttggattt gtaccattaa actataataa ctacaacacc ctactttaca 720

ttatccactt aaacacacgg aa 742

<210> 285

<211> 742

<212> DNA

<213> Coxsackievirus B3 (isolate DH09Y/JS/2012)

<400> 285

ttaaaacagc ctgtgggttg ttcccaccca cagggcccac tgggcgctag cacactggta 60

ttgcggtacc tttgtgcgcc tgttttatcc gccctcccca gcagtaacct agaagcatga 120

ataatgtggt caataggaag ctcagtaaac caactgggtc ttgaccaaac acttctgtta 180

ccccggactg agtatcaaga agctgctcgc gtggctgaag gagaaaacgt tcgttacccg 240

gccaattact tcgagaaacc cagtaccacc atggaagttg cgcagcgttt cgctccgcac 300

aaccccagtg tagatcaggt cgatgagtca ccgcaatcct cacgggtgac cgtggcggtg 360

gctgcgctgg cggcctgccc atggggcaac ccatgggacg cttcaatact gacatggtgc 420

gaagagtcta ttgagctaat tggtagtcct ccggcccctg aatgcggcta atcctaactg 480

cggagcagat atccacacac cagtggacag tctgtcgtaa tgggcaactc tgcagcggaa 540

ccgactactt tgggtgtccg tgtttccttt tatctttata ttggctgctt atggtgacaa 600

ttgagagatt gttaccatat agctattgga ttggccatcc ggtgagcaac agagcaattg 660

tattcctctt tgtaggattt gtgccgttga attacaacaa ctacaacacc ttactctaca 720

tcatccactt aaacacgcgg aa 742

<210> 286

<211> 731

<212> DNA

<213> Coxsackievirus B4 (isolate B401)

<400> 286

gtgggttgtt cccacccgca gggcccactg ggcgctagca cactggtatc acggtacctt 60

tgtgcgcctg ttttacctcc ctccccccca gatgcaactt agaagcagca cttcacgacc 120

aatagcaggc gtggcatacc agctatgtct tggtcaagca cttctgtaac cccggactga 180

gtatcaataa actgctcacg cggtcgaaag agaaaacgtc cgttacccgg ctaactactt 240

cgagaaacct agtagtgcca tgaaagttgc gaagtgtttc gctcagcaca tccccagtgt 300

agatcaggtc gatgagtcac cgcattcccc acaggtaact gtggcggtgg ctgcgttggc 360

ggcctgccca tggggtgacc catgggacgc ttcaatacag acatggtgtg aagagtctat 420

tgagctagtt agtagtcctc cggcccctga atgcggctaa tcctaactgt ggagcatgtg 480

cccccactcc agggggtagc gtgtcgtaac gggtaactcc gcagcggaac cgactacttt 540

gggtgtccgt gtttcctttt attcttacat tggctgctta tggtgacaat tgaaagattg 600

ttaccatata gctattggat tggccacccg gtgacaaaca gagctataat ctaccaattt 660

gtgggatttg ttccaataag tttgagagtc ttaaagacac ttaatttgat attactactc 720

aatagtgcaa a 731

<210> 287

<211> 739

<212> DNA

<213> Coxsackievirus B4 (isolate CV-B4/P11/2013/China)

<400> 287

ttaaaacagg ctgtgggttg gccatccggt gagcccactg ggcgctagca cactggtatc 60

acggtacctt tgtgcgcctg ttttacttcc cttccctata tgtaacttag aagcagcact 120

ctacgaccaa tagtaggcgt ggcacaccag ccatgtcttg gtcaagcact tctgtaaccc 180

cggaccgagt atcaatagac tgctcacgcg gtcgaaggag aaaacgtccg ttacccgacc 240

aactacttcg agaaacctag taacgccatg aaaattgcga ggtgtttcgc tcagcacgcc 300

cccagtgtag atcaggtcga tgagtcaccg cgttccccac aggtgactgt ggcggtggct 360

gcgttggcgg cctgcccatg gggtgaccca tgggacgctt caatacggac atggtgtgaa 420

gagtctattg agctagttgg tagtcctccg gcccctgaat gcggctaatc ctaactgtgg 480

agcacgcgcc cccgctccag agggtagcgt gtcgtaacgg gtaactccgc agcggaaccg 540

actactttgg gtgtccgtgt ttccttttat tcttatactg gctgcttatg gtgacaattg 600

aaagattgtt accatatagc tattggattg gccacccggt gacaaacaga gcaataattt 660

atcaatttgt agggtttgtt ccaataaatt tgaaagtctt gaagacactt aatttaatat 720

tactactcaa tagtgcaaa 739

<210> 288

<211> 744

<212> DNA

<213> Coxsackievirus B4 (isolate Edwards CB4)

<400> 288

ttaaaacagc ctgtgggttg tacccaccca cagggcccac tgggcgttag cacactggta 60

tcccggtacc tttgtgcgcc tgttttatat ccccttcccc atgttgtaac ttagaagcaa 120

tacaatttcg gtcagtagct gacgcgacaa accagttgcg ttttgaccaa gcacctctgt 180

gaccccggac cgagtatcaa taagctgctt gcgcggctga aggagaaacc gttcgttacc 240

cggctaacta cttcgaaaac cctagtaaca ccatgaacgt tgcggagtgt ttcgctcagc 300

acttcccccg tgtagttcag gtcgatgagt caccgcgttc tccacgggtg accgtggcgg 360

tggctgcgtt ggcggcctgc ctgtggggca acccacagga cgctctaata cagacatggt 420

gcgaagagtc tattgagcta gttagtagtc ctccggcccc tgaatgcggc taatcctaac 480

tgcggagcac atgcccacaa accagtgggt ggtgtgtcgt aacgggtaac tctgcagcgg 540

aaccgactac tttgggtgtc cgtgtttcta tttattccta tactggctgc ttatggtgac 600

aattgagaga ttgttgccat atagctattg gattggccat ccggtgtcga atagagcaat 660

tatttaccta tttgttggtt tcataccctt aaaccttaaa gtttttaaga ctcttagtct 720

tatactacaa tttaactcag taaa 744

<210> 289

<211> 730

<212> DNA

<213> Coxsackievirus B5 (isolate B501)

<400> 289

gtgggttgtt cccacccata gggcccactg ggcgccagca cactggtatc acggtacctt 60

tgtgcgcctg ttttacctgc ccttccccaa cgtaacctag aagcatgatc catgcggtca 120

atagccggct cggtgcacca accgggtcac gaccaagcac ttctgttacc ccggactgag 180

tatcaatagg ctgctcgcgt ggctgaagga gaaaacgttc gttacccggc caattacttc 240

gagaaaccta gtaccacctt gaaagttgcg cagcgtttcg ttccgcacaa ccccagtgta 300

gatcaggtcg atgagtcacc gcattcccca cgggcgaccg tggcggtggc tgcgttggcg 360

gcctgcccat ggggtgaccc atgggacgct tcaatactga catggtgcga agagtctatt 420

gagctaattg gtagtcctcc ggcccctgaa tgcggctaat cctaactgcg gagcagatac 480

ccacgagcca gtgggcagtc tgtcgtaatg ggtaactctg cagcggaacc gactactttg 540

ggtgtccgtg tttcctttta actcaatttt ggctgcttat ggtgacaatt gaaagattgt 600

tgccatatag ctattggatt ggccatccgg tgacaaacag agcaattgtc tacctttttg 660

ttggttttgt tccaataaat tttaaagttt taaggaccat taacttaata ttggcactta 720

atacagcaaa 730

<210> 290

<211> 711

<212> DNA

<213> Coxsackievirus B5 (strain USA/MI/2009-23030)

<400> 290

agggcccact gggcgccagc actctggtat cacggtacct ttgtgcgcct gttttaaaac 60

cctctcccca atttgaaact tagaagcaat acacctcgat caatagtagg catgacacgc 120

cagccatgtc ttgatcaagc acttctgttt ccccggactg agtatcaata aactgcttgc 180

gcggtcgaag gagaaaacgt ccgttacccg actaactact tcgagaaacc cagtaacacc 240

atggaaattg cggagtgttt cactcagcac attcccagtg tagatcaggt cgatgagtca 300

ccgcattccc cacgggtgac cgtggcggtg gctgcgctgg cggcctgccc atggggcaac 360

ccatgggacg cttcaatatg gacatggtgt gaagagtcta ttgagctagt tagtagtcct 420

ccggcccctg aatgcggcta atcctaactg cggagcacgt gcctccattc cagggggtgg 480

cgtgtcgtaa cgggcaactc tgcagcggaa ccgactactt tgggtgtccg tgtttctttt 540

aattttatac tggctgctta tggtgacaat tgaaagattg ttgccatata gctattggat 600

tggccatccg gtatccaaca gagcaattgt gtaccttttt gttggatttg taccacttac 660

cagaacaagt tttcatacac tgtgttacat tattagacta aacacagaaa a 711

<210> 291

<211> 742

<212> DNA

<213> Coxsackievirus B6 (isolate 99148/XZ/CHN/1999)

<400> 291

ttaaaacagc ctgtgggttg atcccaccca cagggcccaa cgggcgctag cacactggta 60

ttacggtacc tttgtgcgcc tgttttatcc acccctcccc aacgcaactt agaagcaaag 120

gaatgatgat caacaggaag ctcagtatgc caactgagtg atgatcaagc acttctgtta 180

ccccggaccg agtatcaata agctgctcgc gcggctgaag gagaaaacgt tcgttacccg 240

gccagctact tcgagaagcc cagtaccacc atgaaagttg cgcggcgttt cgctccgcac 300

aaccctagtg tagatcaggt cgatgagtca ccgcgttccc cacgggcgac cgtggcggtg 360

gctgcgttgg cggcctgccc atggggtaac ccatgggacg cttcaatact gacatggtgc 420

gaagagtcta ttgagctagt tggtagtcct ccggcccctg aatgcggcta atcctaactg 480

cggagcagat acccacgaac cagtgggcag tctgtcgtaa tgggcaactc tgcagcggaa 540

ccgactactt tgggtgtccg tgtttccttt tattcattta ttggctgctt atggtgacaa 600

ttgagagatt gttaccatat agctattgga ttggccatcc ggtgacaaac agagcaattg 660

tttacctatt tgtgggattc ataccactag atcacaaagt tctaaacacc ctcaattaca 720

ttcttgtact taataaatta aa 742

<210> 292

<211> 718

<212> DNA

<213> Coxsackievirus B6 (strain LEV15)

<400> 292

cacccacagg gcccactggg cgctagcact ctggtattgc ggtacctttg tgcgcctgtt 60

ttacccaccc tcccccaacg taacttagaa gcctgacata tacggtcaca agccagccca 120

gtacgccaac tgggtaccga ccaagcactt ctgttacccc ggactgagta tcaataagct 180

gccaaagcgg ctgaaggaga aaacgttcgt tatccggcca attacttcga gaaacccagt 240

aacaccatga aagttgcgta gcgtttcatt cagcacaatc ccagtgtaga tcaggtcgat 300

gagtcaccgc attccccaca ggcgactgtg gcggtggctg cgttggcggc ctgcccatgg 360

ggcaacccat gggacgcttc aatactgaca tggtgcgaag agtctattga gctaattggt 420

agtcctccgg cccctgaatg cggctaatcc taactgcgga gcaggcactc gcagaccagc 480

gagcagcttg tcgtaatggg caactccgca gcggaaccga ctactttggg tgtccgtgtt 540

tcctatttcc tttacactgg ctgcttatgg tgacaattga gaaattgtta ccatatagct 600

attggagtgg ccatccggtg actaacagag caataatata tttgtttgtt ggctttgtgc 660

cacttaattt gaaggttgtt aagacgctca atctgatact acttctaaat aaagcaaa 718

<210> 293

<211> 703

<212> DNA

<213> Coxsackievirus A9 (strain A744/YN/CHN/2009)

<400> 293

attgggcgca gcactctggt atcacggtac ctttgtgcgc ctgttttata caccctcccc 60

gaatgtaact tagaagcttc acctagatgg tcaatagaag gtccagcaca ccagttggat 120

tccgaccaag cacttctgtt accccggact gagtatcaat aggccgtttg cacggctgaa 180

ggagaaaacg ttcgttaccc ggccaattac ttcgagaaac ctagtaacac catgaaagtt 240

gcgcagtgtt tcgctccgca cgaccccggt gtagatcagg tcgatgagcc accgcgttcc 300

ccacgggcga ccgtggcggt ggctgcgctg gcggcctgcc catggggcaa cccatgggac 360

gcttcaatac tgacatggtg cgaagagtct attgagctaa ttggtagtcc tccggcccct 420

gaatgcggct aatcctaact gcggagcagg caccctcaag ccagagggca gcttgtcgta 480

acgggcaact ctgcagcgga accgactact ttgggtgtcc gtgtttcctt ttgttcttga 540

actggctgct tatggtgaca attgaaagat tgttaccata tagctattgg attggccatc 600

cagtgacaaa cagagcgatt atatatttgt ttgttggttt tgttccatta aactataaag 660

ccttaaacac acttaaattt gtcttactac tcaactcagc aaa 703

<210> 294

<211> 711

<212> DNA

<213> Coxsackievirus A9 (isolate 2-MRS2013)

<400> 294

agggcccact gggcgctagc acactggtat cacggtacct ttgtgcgcct gttttatttc 60

cccatccccg ccgtaactta gaagtctcac ttaagcggtc aatagatagc ccagtaaacc 120

aactgggttc cgaccaggca cttctgttac cccggaccga gtatcaatag gctactcacg 180

tggccgaagg agaaaacgtt cgttacccgg ccaattactt cgagaaactt agtaacacca 240

tggaagttgc gcagtgtttc gctccgcaca accccagtgt agatcaggtc gatgagtcac 300

cgcgtacccc acgggcgacc gtggcggtgg ctgcgttggc ggcctgccca tggggcaacc 360

catgggacgc ttcaataccg acatggtgcg aagagtctat tgagctaatt ggtagtcctc 420

cggcccctga atgcggctaa tcctaactgc ggagcagacg cctacgaacc agtgggtagt 480

ctgtcgtaac gggcaactct gcagcggaac cgactacttt gggtgtccgt gtttcctttt 540

attcttacac tggctgctta tggtgacaat tgagagattg ttaccatata gctattggat 600

tggccatccg gtgacaaaca gagctattgt ttatctattt attggtttca taccactaaa 660

cttcaaggtc ctaggaaccc tcaatttaat attactactt aatagtgcaa a 711

<210> 295

<211> 698

<212> DNA

<213> Coxsackievirus A1 (clone V18A)

<400> 295

gataggttgt tccsacccca gaggcccacg tggmggmtag taykhkggta tcacggtacc 60

tttgtacgcc tgttttacat cccctcccca agtaacttta gaagtaaatc ataaagttca 120

atagaagggg gtgcaagcca gcaccaccac gaacaagcac ttctgtttcc ccggtgaagc 180

cgtatagact gcccccgcgg ttgaaaatgg cagatccgtt atccgctcac gtacttcgag 240

aagcctagta tcaccttgga atcttcgacg cgttgcgctc agcactctac cccgagtgta 300

gcttaggtcg atgagtctgg gcatacccca ccggtgacgg tggcccaggc tgcgttggcg 360

gcctacccat ggttaacacc atgggacgct agttgtgaac aaggtgtgaa gagcctattg 420

agctacctga gagtcctccg gcccctgaat gcggctaatc ccaaccacgg agcaagtgct 480

cacaaaccag tgggtagctt gtcgtaacgc gcaagtctgt ggcggaaccg actactttgg 540

gtgtccgtgt ttccttttat ttttatcatg gctgcttatg gtgacaatct aagattgtta 600

tcatatagct attggattgg ccatccggtg atttttgaaa gtgatatttg ccttgctatt 660

tcattgcatt tgcttactta taatactgca aactcaca 698

<210> 296

<211> 711

<212> DNA

<213> Coxsackievirus A1 (isolate KS-ZPH01F/XJ/CHN/2011)

<400> 296

ttaaaacagc tctggggttg ttcccacccc agaggcccac gtggcggcta gtacactggt 60

atcacggtac ctttgtacgc ctgttttata tcccctcccc catgcaactt tagaagtaat 120

tcaaatagtt caatagaggg ggtacaaacc agtaccacca cgaacaagca cttctgtttc 180

cccggtgagg ctatatagac tgttcccacg gtcgaaggtg gctgatccgt tatccgcttg 240

agtacttcga gaagcctagt accacctttg gatcttcgat gcgttgcgct cagcactcta 300

ccccgagtgt agcttaggct gatgagtctg ggcatacccc accggcgacg gtggcccagg 360

ctgcgttggc ggcctacccg tggctaacgc catgggacgc tagttgtgaa caaggtgtga 420

agagcctatt gagctactta agagtcctcc ggcccctgaa tgcggctaat cccaaccacg 480

gagcaagtgc tcacaaacca gtgggtggct tgtcgtaacg cgcaagtctg tggcggaacc 540

gactactttg ggtgtccgtg tttcctttta tttttatcat ggctgcttat ggtgacaatc 600

taagattgtt gtcatatagc gtttggattg gccatccggt gacttcgaaa gcgatatatt 660

tgactcattg tcttactgaa tttacctatt tattacactt gtataacaat a 711

<210> 297

<211> 747

<212> DNA

<213> Coxsackievirus A11 (isolate CV-A11_66122)

<400> 297

ttaaaacagc tctggggttg ttcccacccc agaggcccac gtggcggcca gtacaccggt 60

attacggtac ccttgtacgc ctgttttata ctcccttccc cctgtaactt agaagcaata 120

aaccaagttc aacagaaggg ggtacaaacc agtaccacca cgaacaagca cttctgtttc 180

cccggtgagg tcgtatagac tgtccccacg gtcgaaaacg actaatccgt tatccgctct 240

tgtacttcga gaagcctagt atcatcttgg aatcttcgat gcgttgcgct cagcactcga 300

ccccggagtg tagcttaggc cgatgagtct ggacgttccc caccggcgac ggtggtccag 360

gctgcgttgg cggcctacct gtggcccaaa gccacaggac gctagttgtg aacaaggtgt 420

gaagagccta ttgagctaca tgagagtcct ccggcccctg aatgcggcta atcctaacca 480

cggagcaggt agttgcaatc cagtaactgg cctgtcgtaa tgcgtaagtc tgtggcggaa 540

ccgactactt tgggtgtccg tgttyccttt tatttttata atggctgctt atggtgacaa 600

tcattgattg ttatcataaa gcgatttgga ttggccatcc ggtgagaatt agatcaatta 660

tctacttatt tgttggattt acacccttat ctatatacac actaaaccta attagaatta 720

ccttgttaat tagacattat tatcaca 747

<210> 298

<211> 691

<212> DNA

<213> Coxsackievirus A13 (clone V4B)

<400> 298

ggtattacgg tacccttgta cgcctgtttt atactcccct cccccgtaac ttagaagact 60

tcaaaatcaa agttcaatag gagggggtac aaaccagtac caccacgaac aagcacttct 120

gtttccccgg tgatgttgta tagactgtct ccacggtcga aaacgactga tccgttatcc 180

gctctagtac ttcgagaagc ccagtaccac cttggaatct tcgatgcgtt gcgctcagca 240

ctcaatccca gagtgtagct taggttgatg agtctggacg tccctcaccg gcgacggtgg 300

tccaggctgc gttggcggcc tacctgtggc ccaaagccac aggacgctag ttgtgaacaa 360

ggtgcgaaga gcctattgag ctactagaga ttcctccggc ccctgaatgc ggctaatccc 420

aaccacggag caggtactca caacccagtg agcagcctgt cgtaacgcgc aagtctgtgg 480

cggaaccgac tactttgggt gtccgtgttt ccttttattt ttatatggct gcttatggtg 540

acaatcattg attgttatca taaagcgaat tggattggcc atccggtgaa agttagactt 600

atcatttact tgtttgttgg attcactcct ttgactaaat tcacccctga catcatctta 660

gttgtgatac tgattaggag acttcatcat a 691

<210> 299

<211> 745

<212> DNA

<213> Coxsackievirus A13 (strain BAN01-10637)

<400> 299

ttaaaacagc ctgtgggttg ttcccacccc agaggcccac gtggcggcta gtacwcyggt 60

atcacggtac ctttgtacgc ctgttttata tcccctcccc atgcaacttt agaagcaatt 120

caacaagttc aatagaaggg ggtacaaacc agtaccacca cgaacaagca cttctgtttc 180

cccggtgatc tcgcataagc tgtgcccacg gctgaaggca agtgatccgt tatccgcttg 240

ggtacttcga gaagcctagt accaccttgg gatcttcgat gcgttgcgct cagcactcta 300

ccccgagtgt agcttgggcc gatgagtctg ggcattcccc accggtgacg gtggcccagg 360

ctgcgttggc ggcctaccca tggttaacac catgggacgc tagttgtgaa caaggtgtga 420

agagcctatt gagctactca agaatcctcc ggcccctgaa tgcggctaat cccaaccacg 480

gagcaggtgc ccccaaacca gagggtggcc tgtcgtaacg cgcaagtctg tggcggaacc 540

gactactttg ggtgtccgtg tttcctttta tctcttatat ggctgcttat ggtgacaatc 600

atagattgtt atcataaagc gaattggatt ggccatccgg tgaagtacaa acacattatc 660

tacctatttg ttggatttac tcctctcaca ttcaacactc ccaagttaat acaaattgtt 720

cttttaatta gggaaataca ccaca 745

<210> 300

<211> 714

<212> DNA

<213> Coxsackievirus A19 (strain 2019103106/XX/CHN/2019)

<400> 300

ttttttaaaa cagctctggg gttgttccca ccccagaggc ccacgtggcg gctagtactc 60

tggtattaag gtacctttgt acgcctgttt tatctcccct cccccaagta actttagaag 120

taattcaaac agttcaatag agggggtaca aaccagtacc accacgaaca agcacttctg 180

tttccccggt gaagctgtat agactgtacc cacggtcgaa aacggtagat ccgttatccg 240

ctcaagtact tcgagaagcc tagtattacc ttggaatctt cgatgcgttg cactcagcac 300

tctacccccg agtgtagctt aggtcgatga gtctgggcat accccaccgg taacggtggc 360

ccaggctgcg ttggcggcct acccatggct aacgccatgg gacgctagtt gtgaacaagg 420

tgtgaagagc ctattgagct acttgagagt cctccggccc ctgaatgcgg ctaatcccaa 480

ccacggagca agtgcctgca tgccagcagg tagcttgtcg taacgcgtaa gtctgtggcg 540

ggaccgacta ctttgggtgt ccgtgtttcc ttttattctt attatggctg cttatggtga 600

caatctaaga ttgttatcat atagcttttg gattggccat ccggtaatta ttcgactttc 660

aaacgctcgt ttatcttatt tcatcacaca tccgtttgat taattgacta taca 714

<210> 301

<211> 715

<212> DNA

<213> Coxsackievirus A19 (strain 8663)

<400> 301

ttaaaacagc tctggggttg ttcccacccc agaggcccac gtggcggcta gtactctggt 60

attacggtac ctttgtacgc ctgttttaca ctcccttccc tacgcaactt tagaagttta 120

acataaagtt caatagaggg ggtacaaacc agcaccacca cgaacaagca cttctgtttc 180

cccggtgaaa ctgcatagac tgtaaccacg gttgaaagcg gttgatccgt tatccgcttg 240

agtacttcga gaagcctagt accaccttgg aatctccgat gcgttgcgct ccacactcta 300

ccccgagtgt agcttaggcc gatgagtctg ggcgttcccc accggcgacg gtggcccagg 360

ctgcgttggc ggcctaccca tggttaacgc catgggacgc tagttgtgaa caaggtgtga 420

agagcctatt gagctactca agagtcctcc ggcccctgaa tgcggctaat cccaaccacg 480

gagcgagtgc tcacaatcca gtgagtggct tgtcgtaacg cgcaagtctg tggcggaacc 540

gactactttg ggtgtccgtg tttcctttta tttttactat ggctgcttat ggtgacaatc 600

taggattgtt atcatatagc ttttggattg gccatccggt gacttctaac gttcaagtag 660

tttactcgct taattttcta aacacattct cagttgttga attataaact tcaca 715

<210> 302

<211> 745

<212> DNA

<213> Coxsackievirus A20 (strain CAM1976)

<400> 302

ttaaaacagc tctggggttg ttcccacccc agaggcccac gtggcggcta gtactctggt 60

attacggtac ctttgtacgc ctgttttata tcccctcccc ccagtaactt agaaacaaaa 120

aacccaagtt caataggagg gggtacaaac cagtaccacc acgaacaagc acttctgttt 180

tccccggtga ggttgtatag actgtcccca cggtcgaaaa tgactgatcc gttacccgct 240

cctgtacttc gagaagccca gtaacatctt ggaatcttcg atgcgttgcg ctcagcactc 300

aaccccagag tgtagcttag gtcgatgagt ctggacgctc cccaccggcg acggtggtcc 360

aggctgcgtt ggcggcctac ctgtggtcca aagccacagg acgttagttg tgaacaaggt 420

gtgaagagcc tattgagcta caagagagtc ctccggcccc tgaatgcggc taatcccaac 480

catggagcag atgattgcaa accagcaatt ggtctgtcgt aatgcgtaag tctgtggcgg 540

aaccgactac tttgggtgtc cgtgtttcct ttttactctt taatggctgc ttatggtgac 600

aatcacggat tgttatcata aagcgaattg gattggccat ccggtgagaa tcagaacgat 660

tatttcttcc tcgtggattt gtaccaataa caccttacac accgcactta atcagagtta 720

tattgtgtga gatttcatta ccata 745

<210> 303

<211> 689

<212> DNA

<213> Coxsackievirus A21 (isolate 12MYKLU412)

<400> 303

caccccagag gcccacgtgg cggctagtac tctggtatca cggtaccttt gtacgcctgt 60

tttatatccc ttcccccgta actttagaag cttaacaaaa gttcaataga aggggtgcaa 120

accagtacct ccacgaacaa gcacttctgt ctccccggtg aagtcacata gactgtaccc 180

acggtcaaaa gtgattgatc cgttatccgc ttgagtactt cgagaagcct agtatcacct 240

tggaatcttc gatgcgttgc gctcaacact ctaccccggg tgtagcttag gctgatgagt 300

ctgggcatcc cccaccggtg acggtggccc aggctgcgtt ggcggcctac ccatggctaa 360

cgccatggga cgctagttgt gaacaaggtg tgaagagcct attgagctac tcaagaatcc 420

tccggcccct gaatgcggct aatcccaacc acggagcaat cgctcacgat ccagtgagta 480

ggttgtcgta atgcgcaagt tcgtggcgga accgactact ttgggtgtcc gtgtttccct 540

ttttacttat actggctgct tatggtgaca atttacagat tgttaccata tagctattgg 600

attggccatc cagtattgtg ttacatatat taaagtactt ttccacaagc aacaactaca 660

ttgtgtttga accacaatag ccactacaa 689

<210> 304

<211> 736

<212> DNA

<213> Coxsackievirus A21 (strain NIV17-608-2)

<400> 304

ccggggttgt tcccaccccg gaggcccacg tggcggctag tactctggta ccacggtacc 60

cttgtacgcc tgttttatat cccctcccca cgcaacttta gaagaaattc accaagttca 120

ataggggggg tacgaaccag taccaccacg aacaagcact tctgtttccc cggtgatttt 180

acataggctg tacccacggc tgaaagtgaa tgatccgtta cccgcttgag tacttcgaga 240

agcctagtac caccttggaa tctccgatgc gttgcgttca gcactctaac ccgagtgtag 300

cttaggctga tgagtctggg cgatccccac cggcgacggt ggcccaggct gcgttggcgg 360

cctacccgtg gctaacgcta cgggacgcta gttttgaaca aggtgtgaag agcctattga 420

gctacccaag aatcctccgg cccctgaatg cggctaatcc caaccatgga gcaagtgccc 480

tcaagccagg gggtggcttg tcgtaacgcg taagtctgtg gcggaaccga ctactttggg 540

tgtccgtgtt tccttttatt tcatatttgg ctgcttatgg tgacaatcac agattgttat 600

cataaagcga attggattgg ccatccagtg aaatataagc tcattgttta tctccttgtt 660

ggatttgttc tgttaggaaa ttcctcaata aagttgactc gggtaatctt agtgattaga 720

agtcatcatt tcaaca 736

<210> 305

<211> 712

<212> DNA

<213> Coxsackievirus A22 (strain 438913)

<400> 305

ttaaaacagc tctggggttg atcccacccc agaggcccac gtggcggcta gtactctggt 60

attacggtac ctttgtacgc ctgttttata tcccctcccc caagtaactt tagaagcaat 120

tcaaacagtt caatagaggg ggtacaaacc agtaccacca cgaacaagca cttctgtttc 180

cccggtgaag ctgtatagac tgttcccacg gttgaaaatg gcagatccgt tatccgcttg 240

agtacttcga gaagcctagt atcaccttgg aatcttcgac gcgttgcgct cagcactcta 300

ccccgagtgt agcttaggtc gatgagtctg ggcatacccc accggcgacg gtggcccagg 360

ctgcgttggc ggcctaccca tggccaacgc catgggacgc tagttgtgaa caaggtgaga 420

agagcctatt gagctactta agagtcctcc ggcccctgaa tgcggctaat cccaaccacg 480

gagcaagtgc tcacaaacca gtgagtagct tgtcgtaacg cgcaagtctg tggcggaacc 540

gactactttg ggtgtccgtg tttcctttta tttttactat ggctgcttat ggtgacaatc 600

taagattgtt atcatatagc tgttggattg gccatccggt gatttattga gatttaacgt 660

tcgcctgttt ttcttactac atctacccac cagtttcact catataacaa ca 712

<210> 306

<211> 747

<212> DNA

<213> Coxsackievirus A24 (strain 20693_84_CV-A24)

<400> 306

tttaaaacag ctctggggtt gttcccaccc cagaggccca cgtggcggct agtacactgg 60

tattacggta cccttgtacg cctgttttac acccctttcc cccgtaactt agaagcttta 120

aatccaagtt caacagtcag tggtacaacc cagtactacc acgaacaagc acttctgtta 180

ccccggtgag attgcatagg ccgtccccac ggctaaaagc gattgatccg ttatccgctc 240

ttgtacttcg agaagcctag taagaccttg ggatcctcga tgcgttgcgc tcaacactca 300

accccagagt gtagcttggg ctgatgagtc tggacattcc tcaccggtga cggtggtcca 360

ggctgcgttg gcggcctacc tgtggcccaa agccacagga cgctagttgt gaacaaggtg 420

tgaagagcct attgagctac aagagagtcc tccggcccct gaatgcggct aatcccaacc 480

acggagcagg cgattgcaat ccagcaacta gcctgtcgta acgcgcaagt ctgtggcgga 540

accgactact ttgggtgtcc gtgtttcctt ttattacata atggctgctt atggtgacaa 600

tcacagattg ttgtcataaa gcgaattgga ttggccatcc ggtgagaatt aaaacaatta 660

tttacttatt tgttggattt gttcctatac acacccaaac accttgcttg attgaagtaa 720

ttctgttgat aagacaacac tattaca 747

<210> 307

<211> 734

<212> DNA

<213> Coxsackievirus A15 (strain G-9)

<400> 307

ttaaaacagc tctggggttg ttcccacccc agaggcccac gtggcggcta gtacaccggt 60

attacggtac ccttgtacgc ctgttttata ctccctcccc cccgtaactt agaagtacaa 120

aactcaagtt caataggagg gggtacaaac cagtaccacc acgaacgagc acttctgtct 180

ccccggtgag gttacataga ctgtccccgc ggttgaaagt aaccgatccg ttatccgctc 240

ttgtccttcg agaagcccag taccatcttg gaatcttcga tgcgttgcgc tcaacactca 300

accccagagt gtagcttagg tcgatgagtc tggacactcc ccaccggcga cggtggtcca 360

ggctgcgttg gcggcctacc tgtggcccaa agccacagga cgctagttgt gaacaaggtg 420

tgaagagcct attgagctac aagagagtcc tccggcccct gaatgcggct aatcccaacc 480

acggagcagg tgacttcaag ccagaagttg gcctgtcgta acgcgtaagt ctgtggcgga 540

accgactact ttgggtgtcc gtgtttccct ttattcttac aatggctgct tatggtgaca 600

atcatagatt gttatcataa agcgaattgg attggccatc cggtgagaat cagatcagtt 660

acttacctgt ttgttggatt cactccgttg acactttaca tactaaccct tgtcaataca 720

cctgtattat cata 734

<210> 308

<211> 747

<212> DNA

<213> Coxsackievirus A18 (strain CAM1972)

<400> 308

ttaaaacagc tctggggttg ttcccacccc agaggcccac gtggcggcca gtacactggt 60

attacggtac ctttgtacgc ctgttttatg ctcccttccc ctgcaactta gaagattaca 120

aaccaagttc aatagaaggg ggtgcaaacc agtaccacca cgaacaagca cttctgtttc 180

cccggtgaac ttgtatagac tgtacccacg gtcgaaaacg agtgatccgt tatccgctct 240

tgtacttcga gaagcccagt accttcttgg gatcattgat gcgttgcgct cagcactcga 300

ccccggagtg tagcttaggc tgatgagtct ggacatccct caccggcgac ggtggtccag 360

gctgcgttgg cggcctacct gtggtccaaa gccacaggac gctaaatgtg aacaaggtgt 420

gaagagccta ttgagctaca tgagagtcct ccggcccctg aatgcggcta atcctaacca 480

cggagcaggt ggttgcaaac cagcaattgg cctgtcgtaa tgcgtaagtc tgtggcggaa 540

ccgactactt tgggtgtccg tgtttctctt tatttttaca atggctgctt atggtgacaa 600

tcttagattg ttgtcataaa gcgaattgga ttggccatcc ggtgaagata aagcatatca 660

tttatctatt tattggattc actccattaa actcttggac ttatcattta atacaagtag 720

tgttattgat aagaagtgtt caccaca 747

<210> 309

<211> 612

<212> DNA

<213> Human rhinovirus A2 (strain 12L4)

<400> 309

ttaaaactgg atccaggttg ttcccacctg gatctcccat agggagtggt actctgctat 60

tacggtagcc ttgtatgcca gttttatctc ccctccccca tgtaacttag aagttttaca 120

caaggaccaa tagtcggtaa tcagccagat tactaaaggt caagcacttc tgtttccccg 180

gtcaatgctg atatgctcca acagggcaaa aacagcagcg atcgttatcc gcaaagcgcc 240

tacgcaaagc ttagtagcat ctccgaaatt gtctggttgg tcgttccgcc attttcccct 300

tggtagacct ggcagatgag gctagaaata ccccactggc gacagtgttc tagcctgcgt 360

ggctgcctgc acaccctatg ggtgtgaagc caaacaatgg acaaggtgtg aagagccccg 420

tgtgctcgct ttgactcctc cggcccctga atgtggctaa ccttaaccct gcagctagag 480

cacgcaaacc agcgtgtttc tagtcgtaat gagcaattgc gggatgggac caactacttt 540

gggtgtccgt gtttcacttt ttctttttat attgcttatg gtgacaatat atacatcata 600

tatattgaca cc 612

<210> 310

<211> 568

<212> DNA

<213> Human rhinovirus A2 (strain USA/2018/CA-RGDS-1062)

<400> 310

gggtactctg ctattacggt agccttgtat gccggtttta cctcccctcc cccatgtaac 60

ttagaagttt tacacaagga ccaatagtcg gtaatcagcc agattactaa aggtcaagca 120

cttctgtttc cccggtcaat gctgatatgc tccaacaggg caaaaacagt agcgatcgtt 180

atccgcaaag cgcctacgca aagcttagta gcatctccga aattgtctgg ttggtcgttc 240

cgccattttc cccttggtag accttgcaga tgaggctaga aataccccac tggcgacagt 300

gttctagcct gcgtggctgc ctgcacaccc tatgggtgtg aagccaaaca atggacaagg 360

tgtgaagagc cccgtgtgct cgctttgatt cctccggccc ctgaatgtgg ctaaccttaa 420

ccctgcagct agagcacgca aaccagcgtg tttctagtcg taatgagtaa ttgcgggatg 480

ggaccaacta ctttgggtgt ccgtgtttca ctttttcctt ttatattgct tatggtgaca 540

atatatatac agcatatata ttgacacc 568

<210> 311

<211> 610

<212> DNA

<213> Human rhinovirus A2 (X02316)

<400> 311

ttaaaactgg atccaggttg ttcccacctg gatttcccac agggagtggt actctgttat 60

tacggtaact ttgtacgcca gttttatctc ccttccccca tgtaacttag aagtttttca 120

caaagaccaa tagccggtaa tcagccagat tactgaaggt caagcacttc tgtttccccg 180

gtcaatgttg atatgctcca acagggcaaa aacaactgcg atcgttaacc gcaaagcgcc 240

tacgcaaagc ttagtagcat ctttgaaatc gtttggctgg tcgatccgcc atttcccctg 300

gtagacctgg cagatgaggc tagaaatacc ccactggcga cagtgttcta gcctgcgtgg 360

ctgcctgcac accctatggg tgtgaagcca aacaatggac aaggtgtgaa gagccccgtg 420

tgctcgcttt gagtcctccg gcccctgaat gtggctaacc ttaaccctgc agctagagca 480

cgtaacccaa tgtgtatcta gtcgtaatga gcaattgcgg gatgggacca actactttgg 540

gtgtccgtgt ttcacttttt cctttatatt tgcttatggt gacaatatat acaatatata 600

tattggcacc 610

<210> 312

<211> 618

<212> DNA

<213> human rhinovirus a7 (strain atcc vr-1117)

<400> 312

ttaaaactgg gagtgggttg ttcccactca ctccacccac gtggtgctgt acactgttat 60

tccggtaact ttgtacgcca gtttttccct cccttccctc attatgtaac ttagaagcat 120

gaacatatgg accaataggc aataatcatc cagattattg aaggtcaagc acttctgttt 180

ccccggtcaa taaagatatg ctttacccaa ggcaaaaact tttgagatcg ttacccgcac 240

atcaactacg caaagcctag tatcaccttt gatgtactaa gactggtcgt ttcctgcaac 300

cccagcagta gacctggcag atgaggctgg acgtacccca ctggcgacag tggtccagcc 360

tgcgtggctg cctgctcgcc catatgggcg agaagcctta ttattgacaa ggtgtgaaga 420

gccgcgtgtg cttggtgtga gtcctccggc ccctgaatgt ggctaacctt aaccctgcag 480

ccattgctca taatccaatg agttagtggt cgtaatgcgc aagtgcggga tgggaccaac 540

tactttgggt gtccgtgttt cttatttttc tttcatgtgt gttatatggt gacaatttat 600

agtatataag ttgtcatc 618

<210> 313

<211> 606

<212> DNA

<213> Human rhinovirus A8 (strain ATCC VR-1118)

<400> 313

ttaaaactgg gttcaggttg ttcccatctg aacttcccac gtggagtagt acactattat 60

tacggtaatt ttgtacgcca gttttacctc cctccccaaa tgtaacttag aagatgtaca 120

caacgaccaa caggcggtgg ttatccaaac catcaaaggt caagcacttc tgtttccccg 180

gtcaatgttg atatgctcca acagggcaaa aacaactgcg atcgttatcc gcaagatgcc 240

tacgcaaagc ttagtaacac tttggaagat ttatggctgg tcgttccact ataccccata 300

gtagacctgg cagatgaggc tagaaatacc ccactggtga cagtgttcta gcctgcgtgg 360

ctgcctgcgc accctatggg tgcgaagcca tacattggac aaggtgtgaa gagccccgtg 420

tgctcatctt gagtcctccg gcccctgaat gtggctaacc ttaaccccgc agccagtgca 480

cacaatccag tgtgtttttg gtcgtaatga gtaattgtgg gatgggacca actactttgg 540

gtgtccgtgt ttcacttttt cctttattat gcttatggtg acaatatata catatatatt 600

gacacc 606

<210> 314

<211> 613

<212> DNA

<213> Human rhinovirus A9 (isolate F01)

<400> 314

ttaaaactgg atctgggttg ttcccactca ggtctcccac gtggagtagt acactgttat 60

tacggtaact ttgtacgcca gttttacatc ccctcccccc aagtaactta gaagataagc 120

acacaggcca ataggagatg atcagccaga tcattatggg ccaagcactt ctgtttcccc 180

ggtcgaagtt gatatgctcc aacagggcaa aaacaactga gatcgttatc cgcaaagcgc 240

ctacgcaaag cctagtaact atctggaaat tacttggttg gtcgctccgc cataccccat 300

ggtagacctg gcagatgagg ctagaaattc cccactggcg acagtgttct agcctgcgtg 360

gctgcctgca cacccttttt gggtgtgaag ccaagtaatg gacagggtgt gaagagcccc 420

gtgtgctcgc tttgagtcct ccggcccctg aatgtggcta accttaaccc cgcagctaga 480

gcacacaacc cagtgtgtat ctagtcgtaa tgagcaattg cgggatggga ccaactactt 540

tgggtgtccg tgtttcttgt ttttctttta tatttgctta tggtgacaat atatagtgat 600

atatattggc atc 613

<210> 315

<211> 612

<212> DNA

<213> Human rhinovirus A9 (isolate F02)

<400> 315

ttaaaactgg atctgggttg ttcccactca ggtctcccac gtggagtagt acactgttat 60

tacggtaact ttgtacgcca gttttatatc ccctcccctc aagtaattta gaagataaac 120

acacagacca ataggagatg atcagccaga tcattatggg tcaagcactt ctgtttcccc 180

ggtcgaagtt gatatgctcc aacagggcaa aaacaactga gatcgttatc cgcaaagcgc 240

ctacgcaaag cctagtaact atctggaaat tacttggttg gtcgctccgc cataccccat 300

ggtagacctg gcagatgagg ctagaaattc cccactggcg acagtgttct agcctgcgtg 360

gctgcctgca cacccttttg ggtgtgaagc caagtaacgg acagggtgtg aagagccccg 420

tgtgctcgct ttgagtcctc cggcccctga atgtggctaa ccttaaccct gcagctagag 480

cacacaaccc agtgtgtatc tagtcgtaat gagcaattgc gggatgggac caactacttt 540

gggtgtccgt gtttcttgtt tttcttttat atttgcttat ggtgacaata tatagtgata 600

tatattggca tc 612

<210> 316

<211> 611

<212> DNA

<213> Human rhinovirus A9 (strain ATCC VR-489)

<400> 316

ttaaaactgg atctgggttg ttcccactca gatctcccac gtggagtagt acactattat 60

tacggtaatc ttgtacgcca gttttataat ccccttaccc aagtaactta gaagataaac 120

acaaagacca ataggagatg atcatccaga tcatcatagg tcaagcactt ctgtttcccc 180

ggtcaaggtt gatatgctct aacagggcaa aaacagctga gatcgttatc cgcaaagcgc 240

ctacgcaaag cctagtaact atctggaagt tgcttggttg gtcgctccgc catatcccat 300

ggtagacctg gcagatgagg ctagaaattc cccactggtg acagtgttct agcctgcgtg 360

gctgcctgca caccctctgg gtgtgaaacc aagtaatgga cagggtgtga agagccccgt 420

gtgctcgctt tgagtcctcc ggcccctgaa tgtggctaac cttaaccctg cagctagggc 480

acacaatcca gtgtgtatct agtcgtaatg agcaattgcg ggatgggacc aactactttg 540

ggtgtccgtg tttcttgttt ttcttttatg tttgcttatg gtgacaatat atagtaatat 600

atrttggcat c 611

<210> 317

<211> 608

<212> DNA

<213> Human rhinovirus A10 (strain ATCC VR-1120)

<400> 317

ttaaaactgg atccagattg ttcccatctg gatttcccac gtggagttgt acactattat 60

tccggtaatt ttgtacgcca gttttatgta cccccccctg taacttagaa gttttgaaca 120

aggaccaata ggcagtaacc acccaggttg ctaaaggtca agcacttctg tttccccggt 180

caaagttgat atgctccaac agggcaaaaa caactgagat cgttacccgc aaggtgccta 240

cgcaaagcct agtaacccct tgaacgatgt gtggttggtc gttcggctgt accccacagt 300

agacctggca gatgaggcta gatgttcccc actggcgaca gtgttctagc ctgcgtggct 360

gcctgcgcac tctctgagtg cgaagccata catttgacaa ggtgtgaaga gccccgtgtg 420

ctcaccttga gtcctccggc ccctgaatgt ggctaacctt aaccctgcag ctagtacatg 480

caatccagca tgcagctagt cgtaacgagc aattgcggga cgggaccaac tactttgggt 540

gtccgtgttt cactttttcc ttttatattt gcttatggtg acaatatata gatttatata 600

ttgtcacc 608

<210> 318

<211> 595

<212> DNA

<213> Human rhinovirus A11 (strain RvA11/USA/2021/XHZLKL)

<400> 318

ccacctggat ctcccatgtg gagttgtact ctattattcc ggtaattttg tacgccagtt 60

ttatctcccc ttcccctttt gtaacttaga agatgaactc accgaccaat aggtgacatt 120

tatccaaaat gtcaaaggtc aagcacttct gtttccccgg tcaaggatga tatgctccaa 180

cagggcaaaa acatctgcga tcgttatccg caaaacaact acgcaaagcc tagtaccact 240

tttgaaagta tgtggttggt cgctccacta taaacccaat agtagacctg gcagatgagg 300

ctagaagctc cccactggta acagtgttct agcctgcgtg gctgcctgcg tgcctccagt 360

aggcacgaag ccatatattt gacaaggtgc gaagagcccc gtgtgcttgt tttgagtcct 420

ccggcccctg aatgtggcta accttaaccc tgcagctagt gtgcacaatc cagtgcatgg 480

ctagtcgtaa tgagcaattg cgggatggga ccaactactt tgggtgtccg tgtttcttgt 540

ttttcctttt atttgcttat ggtgacaata tatataatac atatatattg gcatc 595

<210> 319

<211> 594

<212> DNA

<213> Human rhinovirus A11 (strain SCH-107)

<400> 319

ccacgtggga gatccaggtg gagttgtact ctattattcc ggtaattttg tacgccagtt 60

ttatctcccc ttcccttttg taacttagaa gatgaactta ccgaccaata ggtggcattt 120

atccaaaatg tcaaaggtca agtacttctg tttccccggt taaggatgat atgctccaac 180

agggcaaaaa catctgcgat cgttatccgc aaaacaacta cgcaaagcct agtaccactt 240

ttgagagtat gtggttggtc gctccactat aaacccaata gtagacctgg cagatgaggc 300

tagaagttcc ccactggtaa cagtgttcta gcctgcgtgg ctgcctgcgt gcctccagta 360

ggcacgaagc catatatttg acaaggtgtg aagagccccg tgtgcttgtt ttgagtcctc 420

cggcccctga atgtggctaa ccttaaccct gcagctagtg tgcgcaatcc agtgcatggc 480

tagtcgtaat gagcaattgc gggatgggac caactacttt gggtgtccgt gtttcttgtt 540

tttcctttta tttgcttatg gtgacaatat atataataca tatatattgg catc 594

<210> 320

<211> 618

<212> DNA

<213> Human rhinovirus A11(EF173414)

<400> 320

ttcaaactgg atccaggttg ttcccacctg gatctcccac gtggagttgt actctattat 60

tccggtaatt ttgtatgcca gttttatctc cccttccctt ttgtaactta gaagatgaac 120

tcaccgacca ataggtggca tttatccaga atgtcaaagg tcaagcacct tctgtttccc 180

cggtcaaagt tgatatgctc caacagggca aaaacatctg cgatcgttat ccgcaaagca 240

actacgcaaa gcctagtatc acttttgaga gtatgtggtt ggtcgctcca ctataaaccc 300

aatagtagac ctggcagatg aggctagaag tcccccactg gtaacagtgt tctagcctgc 360

gtggctgcct gcgtgcctct ggtaggcacg aagccatata tttgacaagg tgtgaagagc 420

cccgtgtgct tgctttgagt cctccggccc ctgaatgtgg ctaaccttaa ccctgcagct 480

agtgtgcata atccaatgca tggctagtcg taatgagcaa ttgcgggatg ggaccaacta 540

ctttgggtgt ccgtgtttct catttttcct tttatttgct tatggtgaca atatatataa 600

tacatatata ttggcatc 618

<210> 321

<211> 604

<212> DNA

<213> Human rhinovirus A12 (isolate p211)

<400> 321

gttgtggttg atcccaccac actgcccact gggcgtagtg ctctcatatt ccggtatgct 60

tgcacgccag ttttattccc ccttccccca ttgtaactta gaagcaaagc acacatgatc 120

aataggagat aacctgccag gttatttaag atcaagcact tctgtttccc cggcaagtgt 180

gaatatgctt tacccaaggc aaaagctaat cttgtcgtta tccgcaaggt gcctacgaga 240

agcctagtaa tgctcaagat gtaatttggc tggtcgctcc gctgtttccc acagtagacc 300

tggcagatga ggctagacat tccccaccag cgatggtggt ctagcctgcg tggctgcctg 360

cacaccctat gggtgtgaag ccaaatttac tgacagggtg tgaagagcct attgtgctca 420

ccttgattcc tccggcccct gaatgtggct aatcctaacc ccgctgctat tgcacacgaa 480

ccagtgtgtg gatagtcgta atgagcaata gtgggatgga accaactact ttgggtgtcc 540

gtgtttcttg tttttcttta atttgtctta tggtcacaat atatatataa catatattgt 600

gatc 604

<210> 322

<211> 613

<212> DNA

<213> Human rhinovirus A12(EF173415)

<400> 322

ttcaaactgg gttgtggttg atcccaccac aactgctcac tgagcgtagt gctctcatat 60

tccggtatgc ttgcacgcca gttttactcc cctcccctca ttgtaactta gaagcaaagc 120

acacctgatc aataggagat aacttgccag gttatctaag atcaagcact tctgtttccc 180

cggcaagtgt gaatatgctt tacccaaggc aaaagctgat cttgtcgtta tccgcaaggt 240

gcctacgaga agcctagtaa tgctcaagat gtggtttgac tggtcgctcc gctgttcccc 300

acagtagacc tggcagatga ggctggacat tccccaccag cgatggtggt ccagcctgcg 360

tggctgcctg cacaccctat gggtgtgaag ccaaatttac tgacagggtg tgaagagcct 420

attgtgctca ccttgattcc tccggcccct gaatgtggct aatcctaacc ccgctgctat 480

tgcacacgaa ccagtgtgta gatagtcgta atgagcaata gtgggatgga accaactact 540

ttgggtgtcc gtgtttcttg tttttcttta tttgtcttat ggtcacaata tatatataac 600

gtatattgtg atc 613

<210> 323

<211> 615

<212> DNA

<213> Human rhinovirus A13 (strain ATCC VR-1123)

<400> 323

ttaaaactgg gttcgggttg ttcccaccca aaccacccac gtggtgtagt acactgttat 60

cccggtaact ttgtacgcca gttttatctc cccaccccct tttgtaactt agaagttatg 120

cacaatgacc aatagtgggc aatcatccag attgtcatcg gtcgagcact cctgtttccc 180

cggttcaagt tgatatgctc taacagggca aaaacaattt gaatcgttac ccgcaaggtg 240

cctacacaga acttagtacc attctggaaa attcttggct ggtcgctcag ttactacccc 300

agtaatagac ctggcagatg gggctagaag ttccccactg gcgacagtgt tctagcctgc 360

gtggctgcct gcacaccctt cttgggtgtg aagcctagaa ttggacaggg tgtgaagagt 420

cgcgtgtgct catcttgagt cctccggccc ctgaatgcgg ctaaccctaa ccctgcagcc 480

attgcccaca acccagtggg tctatggtcg taatgagcaa ttgcgggatg ggaccgacta 540

ctttgggtgt ccgtgtttca ctttttactt attatttgct tatggtgaca atatatagat 600

acatatattg acacc 615

<210> 324

<211> 616

<212> DNA

<213> Human rhinovirus A13 (isolate F03)

<400> 324

ttaaaactgg gtctgggttg ttcccaccca aaccacccac gcggtgtagt acactgttat 60

tccggtaact ttgtacgcca gttttatatc cccacccccc ttttgtaact tagaagttat 120

gcacactgac caatagtggg caatcatcca gattgtcatc ggtcgagcac ttctgtttcc 180

ccggtttgag ttgatatgct ccaccagggc aaaaacaact cagatcgtta tccgcaagat 240

gcctacacag aacttagtac cattctggaa aattcttggc tggtcgttca attactaccc 300

cagtaataga cctggcagat gaggctagaa gttccccact ggcgacagtg ttctagcctg 360

cgtggctgcc tgcacaccct tctggggtgt gaagcctaga attggacagg gtgtgaagag 420

tcgcgtgtgc tcatcttgag tcctccggcc cctgaatgcg gctaacccta accctgcagc 480

cattgcccac aatccagtgg gtttatggtc gtaatgagca attgcgggat gggaccgact 540

actttgggtg tccgtgtttc actttttact tattatttgc ttatggtgac aatatataga 600

tacatatatt gacacc 616

<210> 325

<211> 525

<212> DNA

<213> Human rhinovirus A15 (isolate 7002)

<400> 325

gttacatttg gggtaactta gaagcatgta cacaacgatc aataggacga tggctatcca 60

agtcatctaa gatcaagcac ttctgtctcc ccggtgagtg tgaatatgct ttacccaagg 120

caaaagctaa tctcatcgtt atccgcaagg tgcctacggg aagcctagta atattctgaa 180

gatattttgg ttggtcgctc agttgcactc acccatgcaa tagacctggc agatggggct 240

aggcgtacct cactggtaac agtggcctag cccgcgtggc tgcctgcaca ccttcgggtg 300

tgaagccatg atattgacag ggtgtgaaga gcctactgtg ctcaccttga gtcctccggc 360

ccctgaatgt ggctaatcct aaccccgtat ccattgcaca caaaccagtg tgtctatggt 420

cgtaatgggt aactacggga tggaaccaac tactttgggt gtccgtgttt cttgtttttc 480

tttaatactg cttatggtga cattatatat agatatagtg tcatc 525

<210> 326

<211> 615

<212> DNA

<213> Human rhinovirus A15 (DQ473493)

<400> 326

ttaaaactgg gttcaggttg atcccacctg aacctcccaa gcggagtagt actctgttat 60

ttcggtaact ttgtacgcca gttttatatc cccctcccca tgtaacttag aagatgagct 120

cacagaccaa tagccggcaa tcatccagat tgtcaatggt caagcacttc tgtttccccg 180

gtcaacgttg atatgctcca acagggcaaa aacaactgag atcgttatcc gcaaagtgcc 240

tacgcaaagc ctagtaatgc tttcgaagtc atttggttgg tcgctccgct gtaacccaca 300

gtagacctgg cagatgaggc tagaaactcc ccactggcga cagtgttcta gcctgcgtgg 360

ctgcctgcac accctttggg tgtgaagcca aattacggac aaggtgtgaa gagccccgtg 420

tgctcacttt gagtcctccg gcccctgaat gtggctaacc ttaaccctgc agctagagtg 480

cacaatccag tgtatttcta gtcgtaatga gcaattgcgg gatgggacca actactttgg 540

gtgtccgtgt ttcactttct ttttttatct tttatttgct tatggtgaca atatattgaa 600

ttatatattg acacc 615

<210> 327

<211> 617

<212> DNA

<213> Human rhinovirus A16 (isolate KC939)

<400> 327

ttaaaactgg atctgggttg ttcccactca gatcacctac atggtgttgt acactattat 60

cacggtaatt ttgtacgcca gttttatacc cccttcccca aatgtaactt agaagcaaag 120

caaactgatc aacagggggc aatcgtccag attgtcaaag atcaagcact tctgtttccc 180

cggtcaaagt tgatatgctc cacaagggca aaaacaactg agatcgttat ccgcaagatg 240

cctacgcaaa gcctagtaat acattgaaag atacttggtt ggtcgctcag ctgttaaccc 300

aacagtagac ctggtggatg aggctagaga ttcccctccg gcgacggagt tctagcctgc 360

gtggctgcct gcacacccat tgggtgtgaa gccaagtatt ggacaaggtg tgaagagccg 420

cgtgtgctca tcttgagtcc tccggcccct gaatgtggct aaccttaaac ctgcagccag 480

tgcacacaaa ccagtgtgta gctggtcgta atgagcaatt gcgggatggg accaactact 540

ttgggtgtcc gtgtttcact ttttcctttt attattgctt atggtgacaa tatatatagc 600

atatatatat tgttatc 617

<210> 328

<211> 625

<212> DNA

<213> Human rhinovirus A16 (HRVPP)

<400> 328

ttaaaactgg atctgggttg ttcccaccca gatcacctac atggtgttgt acactattat 60

tacggtaatc ttgtacgcca gttttatact ccctttccca aatgtaactt agaagcaaag 120

caaactgatc aataggggac aatcatccag attgtcaaag atcaagcact tctgtttccc 180

cggtcaaagt tgatatgctc cacaagggca aaaacaactg agatcgttat ccgcaagatg 240

cctacgcaaa gcctagtaat acattgaaag atacttggtt ggtcgctcag ctgttaaccc 300

aacagtagac ctggtagatg aggctagaga ttcccctccg gcgacggagt tctagcctgc 360

gtggctgcct gcacacccac tgggtgtgaa gccaagtatt ggacaaggtg tgaagagccg 420

cgtgtgctca tcttgagtcc tccggcccct gaatgtggct aaccttaaac ctgcagccag 480

tgcacacaat ccagtgtgta gctggtcgta atgagcaatt gcgggatggg accaactact 540

ttgggtgtcc gtgtccgtgt ttcacttttt ttccttttat tattgcttat ggtgacaata 600

tatatagcat atatatattg ttatc 625

<210> 329

<211> 609

<212> DNA

<213> Human rhinovirus A18 (strain HRVA18/03/ZJ/CHN/2017)

<400> 329

actgaatcca ggttgttccc acctggattt cccacgtgga gtagtacact gttattccgg 60

taactttgta cgccagtttt atatcccctc cccttttgta acttagaaga taagcacaag 120

gaccaatagg tggtggctat cccagtcact aaaggtcaag cacttctgtt tccccggtca 180

atgttgatat gctccaccag ggcaaaaaca acagagatcg ttatccgcaa agtgcctacg 240

caaagcctag tagcactttg aatggtatgt ggttggtcgt tccgctattt cccatagtag 300

acctggcaga tgaggctaga aattccccac tggcgacagt gttctagcct gcgtggctgc 360

ctgcgtgtcc taacggacac gaagccatat acttgacaag gtgtgaagag ccccgtgtgc 420

tcactttgag tcctccggcc cctgaatgtg gctaacctta accctgcagc taaggcacac 480

aacccagtgt gaacttagtc gtaatgagca attgcgggac gggaccaact actttgggtg 540

tccgtgtttc actttttacc ttttataatg cttatggtga caatatataa taatttgtat 600

attggcacc 609

<210> 330

<211> 611

<212> DNA

<213> Human rhinovirus 18 (strain ATCC VR-1128)

<400> 330

ttaaaactga atccaggttg ttcccacctg gatttcccat gtggagtagt acactgttat 60

tccggtaact ttgtacgcca gttttataac cccttcccct tatgtaactt agaagataag 120

cacaaagacc aataggtggt ggctatccca gtcactaaag gtcaagcact tctgtttccc 180

cggtcaacgt tgatatgctc caccagggca aaaacaaccg agatcgttat ccgcaaagtg 240

cctacgcaaa gcctagtagc actttgaata gcatgtggtt ggtcgctcca ctattcccca 300

tagtagacct ggcagatgag gctagaaatc ccccactggc gacagtgttc tagcctgcgt 360

ggctgcctgc gtgtcctaac ggacacgaag ccatatgttt gacaaggtgt gaagagcccc 420

gtgtgctcac tttgagtcct ccggcccccg aatgtggcta accttaaccc tgcagctggg 480

gcatacaatc cagtgtgaac tcagtcgtaa tgagcaattg cgggatggga ccaactactt 540

tgggtgtccg tgtttcactt ttttttataa tgcttatggt gacaatatat aataatttgt 600

atattggcac c 611

<210> 331

<211> 614

<212> DNA

<213> Human rhinovirus 19 (strain ATCC VR-1129)

<400> 331

ttaaaactgg atctgggttg ttcccaccca gatcatccac gtgatgttgt gctctattat 60

tccggtaatt ttgcacgcca gtttttcatc ccttccccct ttgtaactta gaagacttaa 120

acaaatgacc aataggaagc aacaaaccag ttgcgatagg tcaagcactt ctgtttcccc 180

ggtcaatgtt gatatgctcc aacggggcaa aaacagcagc gatcgttatc cgcaaggtgc 240

ctacgcaaag cctagtagcg ctcttgaaat tgtttggttg gtcgctcaag tacaaaccca 300

gtactagacc tggcagatga ggctagaaaa cccccactgg cgacagtgtt ctagcctgcg 360

tggctgcctg cacacccatt tgggtgtgaa gccaaacaac ggacagggtg tgaagagcca 420

cgtgtgctca tcttgattcc tccggcccct gaatgcggct aaccttaaac ctgcagccat 480

tgctcacaat ccagtgagtt ggtggtcgta atgagcaatt gcgggatggg accgactact 540

ttgggtgtcc gtgtttcact ttttaccttg ttttatgctt atggtgacaa tatatagtag 600

tatatattga cacc 614

<210> 332

<211> 591

<212> DNA

<213> Human rhinovirus A20 (strain RvA20/USA/2021/B4Q4QT)

<400> 332

gatctcctac atggagttgt actctattat tccggtaatt ttgtacgcca gttttatctc 60

ccctatcccc tttatgtaac ttagaagttt tgtacacacc gaccaatagg tggtatttaa 120

ccaaaatacc aaaggtcaag cacttctgtt accccggtca agattgatat gctccaccag 180

ggcaaaaaca gtcgcgatcg ttacccgcaa ggcaactacg caaagcctag tatcattttt 240

gatggtgtat ggttggtcgt tccgctatga acctgatagt agacctggca gatgaggcta 300

gaaaatcccc actggtaaca gtgttctagc ctgcgtggct gcctgcgtac cccctgtggg 360

tacgaagccg tacatttgac aaggtgtgaa gagccccgtg tgcttgcttt gagtcctccg 420

gcccctgaat gtggctaacc ttaaccctgc agctagtgtg cacaagccag tgcatagcta 480

gtcgtaatga gcaattgcgg gatgggacca actactttgg gtgtccgtgt ttcttgtttt 540

tcttttatgt ttgcttatgg tgacaatata tttaatacat atattgacac c 591

<210> 333

<211> 611

<212> DNA

<213> Human rhinovirus A22 (strain RvA22/USA/2021/WBLGNP)

<400> 333

ataaaactgg cgtgcaaagt tcccatccag atcacccaag cggtgtagtg ctctgttatt 60

ccggtaactt tgcacgccag ttttattacc cctttcccca gtaacttaga agttttaata 120

cactgaccaa tagggtgcga caaccagtcg tacaaggtca agcacttctg tttccccggt 180

caatgttgat atgctcctcc agggcaaaaa caacagcgat cgttatccgc aagatgccta 240

cgcaaagcct agtagcatct ttgaaaatac ttggttggtc gctcatgtgc aaccccagca 300

ttagacctgg cagatgaggc tagaaattcc ccactggcga cagtgttcta gcctgcgtgg 360

ctgcctgcac accctaacgg gtgtgaagcc tggtattgga caaggtgtga agagccccgt 420

gtgctcatct tgagtcctcc ggcccctgaa tgcggctaac cttaaacctg cagccattgc 480

tcacaatcca gtgagtctat ggtcgtaatg agcaattgcg ggatgggacc gactactttg 540

ggtgtccgtg tttctctttt tacttaaatt tgagcttatg gtgacaatat atagcaatat 600

atattggcat c 611

<210> 334

<211> 523

<212> DNA

<213> Human Rhinovirus A23 (strain RvA23/USA/2021/JZHYZ6)

<400> 334

ccccctcccc catgtaactt agaagttttg cacagagacc aataggcagt aatcatccag 60

attacttacg gtcaagtact tctgtttccc cggtcaaagt tgatatgctc caccagggca 120

aaaacagctg cgatcgttat ccgcaaagcg cctacgcaaa gcttagtagc actctggaag 180

ttgtctggct ggtcgctcca ccattccccc tggtagacct ggcagatgag gctagaaact 240

ccccactggc gacagtgttc tagcctgcgt ggctgcctgc acaccctctg ggtgtgaagc 300

catacaatgg acagggtgtg aagagccccg tgtgctcgct ttgagtcctc cggcccctga 360

atgtggctaa ccttaaccct gcagctagag cgcacaaccc agtgtgtttc tagtcgtaac 420

gagcaattgc gggatgggac caactacttt gggtgtccgt gtttccttgt tttctttaaa 480

tgtttgctta tggtgacaat atatacgtat atatattgac acc 523

<210> 335

<211> 620

<212> DNA

<213> Human rhinovirus A24 (strain RvA24/USA/2021/QZ8RX3)

<400> 335

ttaaaactgg atctaggttg ttaccaccta gatctcctat gtggagttgt actctattat 60

tccggtaatt ttgtacgcca gttttattct cccctccccc cgacgtaact tagaagttga 120

acataccgac caataggtaa catttatcca gaatgttaaa ggtcaagcac ttctgtttcc 180

ccggtcaaag ttgatatgct ccaacagggc aaaaacaacc gcgatcgtta tccgcaaagc 240

aactacgcaa agcctagtat catttttgag agtatgtggt tggtcgctcc actataaacc 300

caatagtaga cctggcagat gaggctagaa gttccccact ggtaacagtg ttctagcctg 360

cgtggctgcc tgcgtgctcc ttgtgagcac gaagccatat atttgacaag gtgtgaagag 420

ccccgtgtgc ttgttttgaa tcctccggcc cctgaatgtg gctaacctta accctgcagc 480

tagcgtgtac agtccagtgc aaagctagtc gtaatgagca attgcgggac gggaccaact 540

actttgggtg tccgtgtttc ttgtttttct tttgtatttg cttatggtga caatatatat 600

acagcatata tattggcatc 620

<210> 336

<211> 588

<212> DNA

<213> Human Rhinovirus A25 (strain RvA25/USA/2021/A8F6KW)

<400> 336

ggtgtagtac tctggtatta tgtacctttg tacgcctgtt cttcccattg taccctccct 60

aaatttccaa cccaagtaac gttagaagtt tcacataaaa gtacaacagg aagcaccaca 120

tccagtggtg ttttgtacaa gcacttctgt ttccccggag cgaggtatag gctgtaccca 180

atgccaaaag cctttaaccg ttatccgcca accaactacg taaaagctag taacatcttg 240

tttgtaactt ggcgttcgat caggtggatt ccccctccac tagtttggtc gatgaggcta 300

ggagttcccc acgggtgacc gtgtcctagc ctgcgtggcg gccaacccag cacttgctgg 360

gacgcccttt tatagacatg gtgtgaagac tcgcatgtgc ttggttgtga ttcctccggc 420

ccctgaatgc ggctaacctt aaccccggag ccttgtgttg caaaccagca acattaaggt 480

cgtaacgagc aattctggga tgggaccgac tactttgggt gtccgtgttt ccaatttttc 540

ttattattgt cttatggtca cagcatattt gataaatata ctgtgatc 588

<210> 337

<211> 614

<212> DNA

<213> Human Rhinovirus A28 (strain RvA28/USA/2021/ADMJHA)

<400> 337

ttaaaactga atctaggttg ttcccaccta gattacccac gtggtgtagt actctattat 60

tacggtaatt ttgtacgcca gttttatgct ccccccccca cttgtaactt agaagtttta 120

aactcaaaga ccaataggcg gtgattatcc aaatcatcaa cggtcaagca cttctgtttc 180

cccggttaga ttgatacgct ccaacagggc gaaaacaact ctaatcgtta tccgcaagat 240

gcctacgcaa agcctagtat catcttcgat tctatttggt tggtcgctcg gctacaaacc 300

cagtagtaga cctggcagat gaggctggaa ataccccact ggcgacagtg ttctagcctg 360

cgtggctgcc tgcacaccct atgggtgtga agccaagtag ttgacaaggt gtgaagagcc 420

ccgtgtgctc atcttgattc ctccggcccc tgaatgtggc taaccttaaa cctgcagcca 480

atatacacaa tccagtgtat tgttggtcgt aatgagtaat tgcgggatgg gaccaactac 540

tttgggtgtc cgtgtttctc tttttacctt tattattgct tatggtgaca gtatatagta 600

tatatattgt cact 614

<210> 338

<211> 597

<212> DNA

<213> Human Rhinovirus A29 (strain RvA29/USA/2021/273658-4)

<400> 338

gttgttccca cctggatctc ctacatggag ttgtactcta ttattccggt aatcttgtac 60

gccagtttta tatcccttcc ccttttgtaa cttagaagtt aaacacacag accaataggt 120

gatggctatc caagtcatta acggtcaagc acttctgtct ccccggttcg aattgatacg 180

ctccaccagg gcgaaaacaa ttcgtatcgt tatccgcaaa gtgactacgc aaagcctagt 240

aataccttga agaacttatg gttggtcgct ccactaaacc ccatagtaga cctggcagat 300

gaggctagaa gtaccccact ggtaacagtg ttctagcctg cgtggctgcc tgcacaccct 360

ctgggtgtga agccatattt ttgacaaggt gtgaagagcc ccgtgtgctc actttgagtc 420

ctccggcccc tgaatgtggc taaccttaac cctgcagcta gtgcatgcaa tccagcatgt 480

tgctagtcgt aacgagcaat tgcgggatgg gaccaactac tttgggtgtc cgtgtttcac 540

tttttacctt ttatttgctt atggtgacaa tatatataat acatatatat tggcatc 597

<210> 339

<211> 601

<212> DNA

<213> Human rhinovirus A30 (strain MCL-18-H-1135)

<400> 339

gatctaggtt gtccccacct agatctccca cagggagtgg tactctgtta ttacggtaac 60

tttgtatgcc agttttatct cccctacccc ttatgcaact tagaagtttt acacaaagac 120

caataggcag taaccatcca ggttacttaa ggtcaagcac ttctgtttcc ccggttgacg 180

ttgatatgct ccaacagggc aaaaacaaca gaaatcgtta cccgcaaagt gcctacgcaa 240

agcctagtaa cactttagaa gttgtctggt tggtcgctcc gccatttccc ctggtagacc 300

tggcagatga ggctagaaat accccactgg cgacagtgtt ctagcctgcg tggctgcctg 360

cacaccctat gggtgtgaag ccatacaatg gacaaggtgt gaagagcccc gtgtgctcac 420

tttgagtcct ccggcccctg aatgtggcta accttaaccc tgcagctagg gcacataatc 480

cagtgtgtat ctagtcgtaa tgagcaattg cgggacggga ccaactactt tgggtgtccg 540

tgtttctctt tttatcctta tatttgctta tggtgacaat atatgaatat atattgacac 600

c 601

<210> 340

<211> 598

<212> DNA

<213> Human rhinovirus A31 (strain RvA31/USA/2021/273760-4)

<400> 340

gtgtactact ccacgtggga agtacgggtg gagtagtaca ctattattac ggtaatcttg 60

tacgccagtt ttataacccc ttcccttttg taacttagaa gtttgaactt aagaccaata 120

ggtggtaatc aaccagatta ctaaaggtca agcacttctg tttccccggt caatattgat 180

atgctccacc agggcaaaaa caattgcgat cgttatccgc aaagtgccta cgcaaagctt 240

agtaacactt tgaacaatat atggttggtc gttccgctat tccccatagt agacctggca 300

gatgaggctg gaaactcccc actggcgaca gtgttccagc ctgcgtggct gcctgcgcac 360

tctcacgagt gcgaagccat atatttgaca aggtgtgaag agccccgtgt gctcactttg 420

agtcctccgg cccctgaatg tggctaacct taaccctgca gctagtgcat acaagccagt 480

atgtagctag tcgtaatgag caattgcggg atgggaccaa ctactttggg tgtccgtgtt 540

tcacttttat ctttattatt tgcttatggt gacagtatat agtgtatata ttgttacc 598

<210> 341

<211> 610

<212> DNA

<213> Human rhinovirus A32 (strain ATCC VR-1142)

<400> 341

ttaaaactgg atccgggttg ttcccacccg gatctcccac gtggagttgt acactattat 60

tacggtaatt ttgtacgcca gttttatatc ccttacccca agtaacttag aagataaaca 120

cacagaccaa taggaggtgg tcatccagat cactaagggt caagcacttc tgtttccccg 180

gtcaaggttg atatgctcca acagggcaaa aacagctgag atcattatcc gcaaagtgcc 240

tacgcaaagc ctagtaacta cctcgaaagt acttgactgg tcgctccgcc ataccccatg 300

gtagacctgg cagatgaggc tagaaatccc ccactggtga cagtgttcta gcctgcgtgg 360

ctgcctgcac accctttggg tgtgaagcca agtaatggac agggtgtgaa gagtcgagtg 420

tgctcacttt gagtcctccg gcccctgaat gtggctaacc ctaaccctgc agctagggca 480

cacaatccag tgtgtatcta gtcgtaatga gcaattgcgg gatgggacca actactttgg 540

gtgtccgtgt ttcttgtttt tcatttataa ttgcttatgg tgacaatata tagtattata 600

tattggcgtc 610

<210> 342

<211> 619

<212> DNA

<213> Human rhinovirus A33 (strain ATCC VR-330)

<400> 342

ttaaaactgg atctaggttg ttcccaccta gatctcctac acggagttgt actctgttat 60

tccggtaact ttgtacgcca gttttatacc cccttccccc tttgtaactt agaagctgaa 120

catactgacc aataggcggc attcaaccag aatgtcaaag gtcaagcact tctgtttccc 180

cggttaaagc tgatatgctc caacagggca aaaacagcct taatcgttat ccgcaagaca 240

actacgcaaa gcctagtatc atctttgaaa gtatgtggtt ggtcgctccg ctataaactc 300

aatagcagac ctggcagatg aggctagaaa ctccccactg gcgacagtgt tctagcctgc 360

gcggctgcct gcgtgcccta tacgggtacg aagccatata tttgacaagg tgtgaagagc 420

cccgtgtgct tgtcttgagt cctccggccc ctgaatgtgg ctaaccttaa ccctgcagct 480

ggtatgcaca atccagtaca tggccagtcg taatgagcaa ttgcgggatg ggaccaacta 540

ctttgggtgt ccgtgtttcc tattttacct ttatatttgc ttatggtgac aatatatata 600

taacatatat attggcatc 619

<210> 343

<211> 611

<212> DNA

<213> Human rhinovirus A34 (strain ATCC VR-1144)

<400> 343

ttaaaactgg gtccaggttg ttcccacctg gacttcccac gtggagttgt gcactgttat 60

tccggtaact ttacacgcca gttttatctc ccttcccaaa ygtaacttag aagatgtaca 120

cagcgaccaa taggtggcaa ctagccaagt tgcttaaggt caagcacttc tgtctccccg 180

gtcaatgttg atacgctctc ccagggcgaa aacaactgag atcgttatcc gcaaggcgcc 240

tacacaaaac ctagtaacat cttgaatggt atgtggctgg tcgttccgct aaaccccata 300

gtagacctgg cagatgaggc tagaaattcc ccactggcga cagtgttcta gcctgcgtgg 360

ctgcctgcgt gtccttacgg acacgaagcc atatacttga caaggtgtga agagtcccgt 420

gtgctcgtct tgagtcctcc ggcccctgaa tgtggctaac cttaaccctg cagccagtgc 480

gcacaaacca gtgtgtagct ggtcgtaatg agtaattgcg ggatgggacc aactactttg 540

ggtgtccgtg tttcactttt taccttttat tatgcttatg gtgacaatat atagatgcat 600

atattgacac c 611

<210> 344

<211> 616

<212> DNA

<213> Human rhinovirus A36(DQ473505.1)

<400> 344

ttaaaactgg gagtgggttg ttcccactca ctccacccat gcggtgttgt acactgttat 60

tacggtaacc ttgtacgcca gttttgccac ccttccccat tatgtaactt agaagtttgt 120

acaacaagac caataggtga taattatcca gattatcaaa ggtcaagcac ttctgtttcc 180

ccggtcgatg aggatatgct ttacccaagg caaaaacctt aaagatcgtt atccccacac 240

tgcctacaca gagcctagta ccacttttga tgtaattagg ttggtcgctc cctgcaaacc 300

cagcagtaga cctggcagat gaggctggac actccccact ggtgacagtg gtccagcctg 360

cgtggctgcc tgctcaccct attgggtgag aagcctactt attgacaaga tgtgaagagc 420

cgcgtgtgct cagtgtgctt cctccggccc ctgaatgtgg ctaaccttaa ccctgcagcc 480

gctgcccata atccaatggg tttacggtcg caacgcgcaa gtgcgggatg ggaccaacta 540

ctttgggtgt ccgtgtttcc tatttttctt ttatttgcat tttatggtga caatttatag 600

tatatagatt gtcgtc 616

<210> 345

<211> 610

<212> DNA

<213> Human rhinovirus A38 (strain ATCC VR-1148)

<400> 345

ttaaaactgg atctgggttg ttcccactca gatctcccat gcggagttgt actctattat 60

tccggtaatc ttgtacgcca gtttttacac cctcccccct tgtaacttag aagaaagtac 120

ataacgacca ataggtggca attaaccaaa ttgtcaacgg tcaagcactt ctgtttcccc 180

ggtcaagatt gatacgctcc aacagggcga aaacaattga gatcgttatc cgcaaagtgc 240

ccacgcaaag cctagtaaca ctttgaaagg tctatggttg gtcgttcagc taaaccccat 300

agtagacctg gcagatgagg ctagaaattc cccactggcg acagtgttct agcctgcgtg 360

gctgcctgca caccctcacg ggtgtgaagc catatactcg acaaggtgtg aagagccccg 420

tgtgctcact ttgagtcctc cggcccctga atgtggctaa ccttaacccc gcagctagtg 480

catacaaacc agtatgtggc tagtcgtaat gagcaattgt gggatgggac caactacttt 540

gggtgtccgt gtttcacttt tttccttttg tttgcttatg gtgacaatat atatagtata 600

tattgacacc 610

<210> 346

<211> 613

<212> DNA

<213> Human rhinovirus A39 (strain ATCC VR-340)

<400> 346

ttaaaactgg atctgggttg ttcccaccta gatcacctac agggtgtagt acactgttat 60

tacggtaact ttgtacgcca gttttatatc ccctccccga tgtaacttag aagcaagcac 120

aaacgtccaa taggcggtga ttatccaaat cacttacgga caagcacttc tgtctccccg 180

gcaactgctg atatgctcca acagggcaaa aacagcggaa gtcgtcatcc gcaaaatgcc 240

tacgcaaagc ctagtatcac tctagaaggt gtgtggttgg tcgctcagcc gtgaacctga 300

cggtagacct ggcagatgag gctagaaata ccccactggc gacagtgttc tagcctgcgt 360

ggctgcctgc acaccctcac gggtgtgaag ccattcattg gacagggtga gaagagccca 420

gtgtgctcat tttgagtcct ccggcccctg aatgtggcta accttaaacc tgcagccaat 480

gcacacaacc cagtgtgtat ttggtcgtaa cgagcaattg cgggatggga ccaactactt 540

tgggtgtccg tgtttctttt tattcttatc atattgccta tggtgacaat atatactgtt 600

atatattgtt atc 613

<210> 347

<211> 612

<212> DNA

<213> Human rhinovirus A40 (strain 7D5)

<400> 347

ttaaaactgg gtccaggttg ttcccacctg gatctcctac atggagttgt actctgttat 60

tccggtaacc ttgtacgcca gttttatatc ccctccccaa atgtaactta gaagttatac 120

acacagacca ataggcggtg atcctccaga tcactaacgg tcaagcactt ctgtctcccc 180

ggtttgaatt gatacgctcc aacagggcga aaacaatttg aatcgttacc cgcaaagtgc 240

ctacgcaaag cctagtagca ctttagaaaa tttatggttg gtcgctcagc tataccccct 300

agtagacctg gcagatgagg ctagaagtac cccactggcg acagtgttct agcctgcgtg 360

gctgcctgca caccctcacg ggtgtgaagc catatatttg acaaggtgtg aagagccccg 420

tgtgctcact ttgagtcctc cggcccctga atgtggctaa ccttaaccct gcagctagtg 480

catgtaatcc aacatgttgc tagtcgtaat gagcaattgc gggatgggac caactacttt 540

gggtgtccgt gtttcacttt ttacctttta tttgcttatg gtgacaatat atataatata 600

tatattggca tc 612

<210> 348

<211> 613

<212> DNA

<213> Human rhinovirus A41 (strain SC9861)

<400> 348

taaaactggg tttgggttga tcccacccaa accacccatg tggtgtagta cactgttatt 60

tcggtaactt tgtacgccag ttttatctcc ccttccccct tcgtaactta gaagtaacac 120

ataatgacca atagcgggca atcatccaga ttgtcactgg tcaagcactt ctgtttcccc 180

ggttcagatt gatatgctcc aacagggcaa aaacagtttg aatcgttacc cgcaagatgc 240

ctacgcagaa cctagtatca cactgaaaga ttcttggttg gtcgttcagt tacatcccca 300

gtaacagacc tggcagatga ggctagaagt tccccactgg cgacagtgtt ctagcctgcg 360

tggctgcctg cacacccatt tgggtgtgaa gcctagaatt ggacagggtg tgaagagtcg 420

agtgtgctca tcttgagtcc tccggcccct gaatgcggct aaccttaacc ccgcagccat 480

tgcccacaaa ccagtgggtt tatggtcgta atgagcaatt gtgggatggg accgactact 540

ttgggtgtcc gtgtttcact ttttacttat tatttgctta tggtgacaat atatagatac 600

atatattgac acc 613

<210> 349

<211> 613

<212> DNA

<213> Human rhinovirus A43 (strain ATCC VR-1153)

<400> 349

ttaaaactgg gtccaggttg ctcccacctg gatcactcac tgagtgttgt atactgttat 60

tacggtaact ttatacgcca gttttatctc cccttacccc aatgtaactt agaagtttgt 120

acataccgac caatagtgag caatcatcca gattgtttaa ggtcaagcac ttctgttacc 180

ccggtcagcg ttgatatgct ccaacagggc aaaaacaaca atgatcgtta tccacaagat 240

gcctacacag agcctagtaa cattctagaa aaattctggt tggtcgctca attgcaaacc 300

cagcaataga cctggcagat gaggctggac attccccact ggcgacagtg gtccagcctg 360

cgtggctgcc tgcacactcc tttggagtgt gaagccataa tttggacagg gtgcgaagag 420

ccgcgtgtgc tcatcttgag tcctccggcc cctgaatgcg gctaacctta aacctgcagc 480

cattgtttgc aatccagcag atrtatggtc gtaatgagca attgcgggat gggaccgact 540

actttgggtg tccgtgtttc ctttatttcc atttatatgc ttatggtgac aatatatgat 600

atatattgac acc 613

<210> 350

<211> 612

<212> DNA

<213> Human rhinovirus A44(DQ473499)

<400> 350

ttaaaactgg gtccaggttg ttcccacctg gatctcctac atggagttgt actctattat 60

cccggtaatc ttgtacgcca gttttatatc ccttcccttt ttgtaactta gaagttaaac 120

acacagacca ataggtggtg gctatccaag tcattaatgg tcaagcactt ctgtttcccc 180

ggtttagatt gatacgctcc accagggcga aaacaattta aatcgttatc cgcaaaatga 240

ctacgcaaag cctagtaata tcttgaagga cttatggttg gtcgctccac taaaccccat 300

agtagacctg gcagatgagg ctagaaatac cccactggag acagtgttct agcctgcgtg 360

gctgcctgca caccctatgg gtgtgaagcc atactttcga caaggtgtga agagccccgt 420

gtgctcattt tgagtcctcc ggcccctgaa tgtggctaac cttaaccctg cagctagtgc 480

atgcaatcca gcatgttgct agtcgtaacg agcaattgcg ggatgggacc aactactttg 540

ggtgtccgtg tttcactttt tccttttatt tgcttatggt gacaatatat atagtacata 600

tatattggca tc 612

<210> 351

<211> 615

<212> DNA

<213> Human rhinovirus A45 (strain ATCC VR-1155)

<400> 351

ttaaaactgg gtcgtggttg ttcccaccac gactatctac ggatgtagtg ctcttgtatt 60

ccggtacgct tgcacgccag ttttgtcacc cccccctaca ttgtaactta gaagattaac 120

acaaagacca ataggcggca atgaaccaca ttgtcaacgg tcaagcactt ctgtttcccc 180

ggtcaatcta gatatgcttt accaaaggca aaaactggag cgatcgttat ccgcaaggtg 240

cctacgggaa acccagtagc attctttatt tgatctggtt ggttgctcag ccgttaaacc 300

caaacggtaa acctggcaga tgaggctgga agatccccac cagcgatggt gttccagcct 360

gcgtggctgc ctgcacaccc gaaagggtgt gaagcctttt caaagacagg gtgcgaagag 420

tctactgtgc tcaccttgat tcctccggcc cctgaatgtg gctaatccta accccgtagc 480

tgtggtgtgc aatccagcac attcgcagtc gtaatgggta actgcgggat ggaaccaact 540

actttgggtg tccgtgtttc tctttttcct tttatatatg cttatggtga caattatacg 600

gatatagttg tcatc 615

<210> 352

<211> 609

<212> DNA

<213> Human rhinovirus A46 (strain RvA46/USA/2021/6EEDHN)

<400> 352

ttaaaactga atctgggttg ttcccaccca gatttcccat gtggagtagt actctattat 60

tccggtaatt ttgtacgcca gttttataac ccctacccta agcaacttag aagataagca 120

cactcgacca atagtgggtg attatccaaa tcatcaaagg tcaagcactt ctgtttcccc 180

ggttgaagtt gatatgcccc accagggcaa aaacaactga gatcgttatc cgcaaaatgc 240

ctacacaaag cctagtatca tcttgaaagg cgcagagttg gtcgttccgc tattccccat 300

agtagacctg gcagatgagg ctagaaaacc cccactggcg acagtgttct agcctgcgtg 360

gctgcctgcg tgccttgcaa ggcacgaagc cctgtgtttg acaaggtgtg aagagccccg 420

tgtgctcatt ttgattcctc cggcccctga atgtggctaa ccttaaccct gcagctagtg 480

cacacaaacc agtgtgtatc tagtcgtaat gagcaattgc gggacgggac caactacttt 540

gggtgtccgt gtttctcttt ttacttttta cttgcttatg gtgacaatat atagatatat 600

attgacacc 609

<210> 353

<211> 612

<212> DNA

<213> Human rhinovirus A47 (strain ATCC VR-1157)

<400> 353

ttaaaactgg gtgtgggttg ttcccaccca cacctcccac gtggagtagt acactgttat 60

cacggtaatt ttgtacgcca gttttataac ccctttccct tttgtaactt agaagtttga 120

acttgagacc mataggcggt ggtcaaccag atcactaaag gtcaagcact tctgtttccc 180

cggtcaacgt tgatatgctc cagcagggca aaaacagytg cgatcgttat ccgcaaagtg 240

cctacgcaaa gcttagtaac atcttgaatg atatatggtt ggtcgttcca ctaaacccca 300

tagtagacct ggcagatgag gctggaaatt ccccactggc gacagtgttc cagcctgcgt 360

ggctgcctgc gcactcttat gagtgcgaag ccatatattt gacaaggtgt gaagagcccc 420

gtgtgctcac tttgagtcct ccggcccctg aatgtggcta accttaaccc tgcagctagt 480

gcatacaatc cagtgtgtag ctagtcgtaa tgagcaattg cgggatggga ccaactactt 540

tgggtgtccg tgtttcactt ttccttttat gatttgctta tggtgacagt atatagttta 600

tatattgttg cc 612

<210> 354

<211> 615

<212> DNA

<213> Human rhinovirus A49 (isolate F04)

<400> 354

ttaaaactgg atctgggttg ttcccacttg gatctcccaa agggagtggt actctgttat 60

tacggtaact ttgtacgcca gttttatcct ccccttaccc catgtaactt agaagttttt 120

cataaagacc aatagttggt aatcatccag attacttaag gtcaagcact tctgtttccc 180

cggtcaatgt tgatatgctc caacagggca aaaacaactg cgatcgttat ccgcaaagcg 240

cctacgcaaa gcttagtagc acctttgaga ttgtttggtt ggtcgttccg ccatttcccc 300

tggtagacct ggcagatgag gctagaagta ccccactggc gacagtgttc tagcctgcgt 360

ggctgcctgc acaccctgtg ggtgtgaagc caaataatgg acaaggtgtg aagagccccg 420

tgtgctcgct ttgagtcctc cggcccctga atgcggctaa ccttaaccct gcagctagag 480

catacaaacc agtgtgtatc tagtcgtaat gagcaattgc gggatgggac cgactacttt 540

gggtgtccgt gtttcacttt ttttttactt ataattgctt atggtgacaa tatatatata 600

gtatatattg acacc 615

<210> 355

<211> 610

<212> DNA

<213> Human rhinovirus A50 (strain ATCC VR-517)

<400> 355

ttaaaactgg gtccaggttg ttcccacctg gatctcctaa tgggagttgt actctgttat 60

tccggtaact ttgtatgcca gttttatctc cctaccccca atgtaactta gaagatgtac 120

acaacgacca ataggtgatg atcaaccaga ttatcaaagg tcaagcactt ctgttttccc 180

ggttagagtt gatatgctcc aacagggcaa aaacaacttt aatcgttatc cgcaaagcgc 240

ctacgcaaag cctagtaaca gcttcgaaga tttatggttg gtcgttccgc tgttccccac 300

agtagacctg gcagatgagg ctagagattc cccactggta acagtgttct agcctgcgtg 360

gctgcctgcg cacccttcgg gtgcgaagcc atatttcgga caaggtgcga agagccccgt 420

gtgctcgctt tgagtcctcc ggcccctgaa tgtggctaac cttaaccctg cagctagtgc 480

atacaaacca gtatgtggct agtcgtaatg agcaattgcg ggatgggacc aactactttg 540

ggtgtccgtg tttctctttt tccttttatt attgcttatg gtgacaatat atagtttata 600

tattggcacc 610

<210> 356

<211> 613

<212> DNA

<213> Human rhinovirus A51 (strain ATCC VR-1161)

<400> 356

ttaaaactgg gtgtgggttg tacccaccca cactgtctac ggacgtagtg ctctcatatt 60

acggtatgtt tgcacgccag tttttacccc ccccccaaat ttgtaacatt agaagtctgc 120

actcttaacc tggtcaatag gaggtagcaa tccatgctat ctaagaccaa gcacttctgt 180

taccccggta tgcggagaat agactccaaa agggttgaag ctccaaatat cgttatccgc 240

acaattacta cacaaagcct agtaatatct tgataagctc tttggttggt cgttcagctg 300

ttacccccag tagacttggc agatgaggct gggtgttccc cactggcgac agtgacccag 360

cctgcgtggc tgcctacaca cctggagaca ggtgtgaagc caaaagtgga caaggtgtga 420

agagccccgt gtgctaattg tgattcctcc ggcccctgaa tgcggctaat ccaaccccac 480

agccaatgca tgtaaaccaa catgtatttg gtcgtaacgg gtaactgtgg gacggaaccg 540

actactttgg gtgtccgtgt ttccttttat ttttrttgct tatggtgaca agtattggta 600

tatatttgtc atc 613

<210> 357

<211> 612

<212> DNA

<213> Human rhinovirus A53(DQ473507)

<400> 357

ttaaaactgg atctgggttg ctcccaccca gattacccaa gtggtgtagt actctgttat 60

tacggtagct ttgtacgcca gttttatcac ccctccccac aatgtaactt agaagtttta 120

cacttacaga ccaataggat gtgattatcc aaatcactaa aggtcaagca cttctgtttc 180

cccggttaga tttgatacgc tccaacaggg cgaaaacaga tctgatcgtt atccgcaaga 240

tgcctacgca aagcttagta gcgctctgaa tgctttttgg ctggttgttc cactacaaac 300

ccagtagtaa acctggcaga tgaggctaga agttccccac tggcgacagt gttctagcct 360

gcgtggctgc ctgcacaccc tatgggtgtg aagccaatca gttgacaggg tgtgaagagc 420

cccgtgtgct catcttgagt cctccggccc ctgaatgtgg ctaaccttaa acccgcagcc 480

aaggtgtgca aaccagcaca ttcttggtcg taacgagtaa ttgtgggatg ggaccaacta 540

ctttgggtgt ccgtgtttct ttttattctt ttacaatgcc tatggtgaca atatatagta 600

tatattgtca tt 612

<210> 358

<211> 611

<212> DNA

<213> Human rhinovirus A54 (strain ATCC VR-1164)

<400> 358

ttaaaactgg gtctaggttg ttcccaccta gacctcctac atggagttgt actctattat 60

ttcggtaatt ttgtacgcca gttttatatc ccctttcccc acgtaactta gaagtttaac 120

acactgacca ataggtggca attaaccaaa ttgctaaagg tcaagcactt ctgtttcccc 180

ggtcaagatt gatacgctct accagggcga aaacaattga gatcgttamc cgcaaagtgc 240

ctacgcgaag cctagtaaca tcttgaagaa tttatggttg gtcgctccac tattccccat 300

agtagacctg gcagatgagg ctagaaattc cccactggcg acagtgttct agcctgcgtg 360

gctgcctgca cacccttacg ggtgtgaagc catatatttg acaaggtgtg aagagcctag 420

tgtgctcact ttgagtcctc cggcccctga atgtggctaa ccttaaaccc gtagctagtg 480

catacaatcc agtgtgtggc tagtcgtaac gagcaattac gggatgggac caactacttt 540

gggtgtccgt gtttcacttt ttccttttat atttgcttat ggtgacaata tatagattat 600

atattgacac c 611

<210> 359

<211> 522

<212> DNA

<213> Human rhinovirus A55(DQ473511)

<400> 359

accttcctcc atgtaactta gaagatgtac actcagacca ataggtgata atcctccaga 60

ttatctaagg tcaagcactt ctgtttcccc ggtctaaact gatatgctcc aacagggcaa 120

aaacagtgca gatcgttatc cgcaggatgc ctacgcaaag tctagtaacc cctttgaaga 180

tatttggttg gtcgctccac tattccccat agtagacctg gcagatgagg ctagaaattc 240

cccactggtg acagtgttct agcctgcgtg gctgcctgca caccctattg ggtgtgaagc 300

caaatattgg acaaggtgtg aagagccccg tgtgctcatc ctgagtcctc cggcccctga 360

atgtggctaa ccttaaccct gcagctagtg catgcaatcc agcatgtagc tagtcgtaat 420

gagcaattgc gggatgggac caactacttt gggtgtccgt gtttcacttt ttaccttata 480

tttgcttatg gtgacaatat atattgcata tatattggca tc 522

<210> 360

<211> 613

<212> DNA

<213> Human rhinovirus A56 (strain ATCC VR-1166)

<400> 360

ttaaaactgr gttcaggttg ttcccacctg gacttcctat gtggagttgt acactgttat 60

cccggtaacc ttgtacgcca gttttataca cccttccctg taacttagaa gttataagca 120

cgaggaccaa taggtagtga ctttccaggt cactaaaggt caagcacttc tgtctccccg 180

gtcaaagttg atatgctctc cccagggcaa aaacaattga gatcgttatc cgcaatgtgc 240

ctacgcaaag cctagtaata tcttagatga tatatggttg gtcgctccgc catttcccat 300

ggtagacctg gcagatgagg ctagaaactc cccactggcg acagtgttct agcctgcgtg 360

gctgcctgcg cactctctga gtgcgaagcc atatatttga caaggtgtga aragccccgt 420

gtgctcacat tgaatcctcc ggcccctgaa tgtggctaac cttaaacctg cagctagtgc 480

acacaaacca gtgtgtagct agtcgtaacg agcaattgcg ggatgggacc aactactttg 540

ggtgtccgtg tttcactttt ttccttttat atttgcttat ggtgacaata tatataatac 600

atatattgac acc 613

<210> 361

<211> 614

<212> DNA

<213> Human rhinovirus A57 (isolate fs ship#1-hrv-57)

<400> 361

ttaaaactgg atccaggttg ttcccacctg gatctcctac gggagttgta ctctgttatt 60

tcggtaacct tgtacgccag ttttatctcc cctcccccaa ttgtaactta gaagcaatac 120

acaaggacca ataggcagtg attaaccaga tcacttaagg tcaagcactt ctgtttcccc 180

ggtctgagtt gatatgctct aacagggcaa aaacaagtca gatcgttatc cgcaaagtgc 240

ctacgcaaag cctagtatca tctttgaaaa tgtatggttg gtcattccgc tgaaccccac 300

agtagacctg gcagatgagg ctagaaaccc cccactggcg acagtgttct agcctgcgtg 360

gctgcctgca cacccttatg ggtgtgaagc catatatttg acaaggtgtg aagagccccg 420

tgtgctcact ttgagtcctc cggcccctga atgtggctaa ccctaaccct gcagctagtg 480

catacaagcc agtatgttgc tagtcgtaac gagcaattgc gggatgggac caactacttt 540

gggtgtccgt gtttcacttt ttacctttat actgcttatg gtgacaatat atatatagca 600

tatatattga cacc 614

<210> 362

<211> 618

<212> DNA

<213> Human rhinovirus A58 (strain ATCC VR-1168)

<400> 362

ttaaaactgg gagtaggttg ttcccaccta ctctacccac gtggtgttgt acactgttat 60

cacggtaact ttgtatgcca gttttgccac ccttccccgt agcgtaactt agaagtttgt 120

acacttagac caataggcga taatcatcca gattatcaaa ggtcaagcac ttctgtttcc 180

ccggtcaatg aggatatgct tttcccaagg caaaaacctt agagatcgtt atccccacat 240

tgcctacaca gagcctagta tcacctttga tgtatttaga ctggtcgctc cctgcaaacc 300

cagcagtaga cctggcagat gaggctggac gttccccact ggcgacagtg gtccagcctg 360

cgtggctgcc tgcccaccca tttgggtggg aagcctaact attgacatgg tgtgaagagc 420

cgcgtgtgct caatgtgcct cctccggccc ctgaatgtgg ctaaccttaa ccccgcagcc 480

gctgcccatg atccagtggg tatacggtcg taacgcgcaa gtgtgggatg ggaccaacta 540

ctttgggtgt ccgtgtttcc tgtttttctt ttttttatgc attttatggt gacaatttat 600

ggtatataga ttgtcatc 618

<210> 363

<211> 614

<212> DNA

<213> Human rhinovirus A59 (strain 16-J2)

<400> 363

ttaaaactgg atccaggttg ttcccacctg gatctcccaa gtggagtggt acactattat 60

cccggtaatt ttgtacgcca gttttataac ccctcccccc cttgtaactt agaagatgta 120

aacacagacc aataggtggt aactaaccaa gttactaaag gtcaagcact tctgtttccc 180

cggctgatat tgatatgctc caccagggca aaaacaatag aagtcgttat ccgcaaagtg 240

cctacgcaaa gcctagtagc actttagatt gtgtatggtt ggtcgctcag ctattccccc 300

tagtagacct ggcagatgag gctagaaata ccccactggt aacagtgttc tagcctgcgt 360

ggctgcctgc gcaccctttc gggtgcgaag ccatatacat gacaaggtgt gaagagcccc 420

gtgtgctcac tttgagtcct ccggctcctg aatgtggcta accttaaacc tgcagccagt 480

gcacataagc caatgtgtag ctggtcgtaa tgagtaattg cgggatggga ccaactactt 540

tggatgtccg tgtttcttgt ttttccttta attttgctta tggtgacaat atatagataa 600

tatatattgg catc 614

<210> 364

<211> 613

<212> DNA

<213> Human rhinovirus A60 (strain ATCC VR-1473)

<400> 364

ttaaaactgg atccaggttg ttcccacctg gatctcctaa cgggagtagt acactattat 60

tacggtaatt ttgtacgcca gttttatgat cccctccccc ttttgtaact tagaagttaa 120

acaaaatgac caataggcgg caatctccca gattgtcaaa ggtcaagcac ttctgtttcc 180

ccggtcaaag ctgatatgct ccaacagggc aaaaacagct gagatcgtta cccgcaaagt 240

gcctacgcaa agcttagtaa cacttttgaa gttacgtggt tggtcgctca gccaaacccc 300

atggtagacc tggcagatga ggctagaagt accccactgg cgacagtgtt ctagcctgcg 360

tggctgcctg cacaccctct gggtgtgaag ccatataatg gacaaggtgt gaagagcccc 420

gtgtgctcac tttgagtcct ccggcccctg aatgtggcta accttaaccc tgcagctagt 480

gcatgcaatc cagtatgtat ctagtcgtaa tgagcaattg cgggatggga ccaactactt 540

tgggtgtccg tgtttccttt ttatccttat tgattgctta tggtgacaat atatagtatt 600

atatattgac rcc 613

<210> 365

<211> 618

<212> DNA

<213> Human rhinovirus A61 (strain SCH-99)

<400> 365

ttaaaactgg gtctgggttg tccccaccca gaccacccac gtggtgttgt acactgttat 60

cccggtaact ttgtacgcca gttttaaact cccttacccc ttttgcaact tagaagttat 120

acacaccgac caacagcaga caatcatcca gattgtttat ggtcaagcac ttctgtctcc 180

ccggtctcct ctgatatgct ccaacagggc aaaaacagag tagatcgtta cccgcaagat 240

gcctacgcaa cgcctagtaa cactttgaag tgattttggt tggtcgctca tttacaaccc 300

cagtaataga cctggcagat gaggctagat gttccccact ggcgacagtg atctagcctg 360

cgtggctgcc tgcacaccct ttgtgggtgt gaagccaaat ttatgacaag gtgcgaagag 420

ccacgtgtgc tcatcttgag tcctccggcc cctgaatgcg gctaacctta accctgcagc 480

cattgctcac aaaccagtga gtatgtggtc gtaatgagaa attgcgggac gggaccgact 540

actttgggtg tccgtgtttc actttttacc ttatttttgc ttatggtgac aatatatata 600

tagatatata ttgacacc 618

<210> 366

<211> 614

<212> DNA

<213> Human rhinovirus A62 (strain ATCC VR-1172)

<400> 366

ttaaaactgg atccaggttg ttcccacctg gatytcctac gtggagttgt actctattat 60

tccggtaatc ttgtacgcca gttttatatt ccctacccct tttgtaactt agaagttaag 120

cacacagacc aataggcggt gaccacccaa gtcactaacg gtcaagcact tytgtttccc 180

cggttaagat tgatacgctc caacagggcg aaaacaatyt aaatcgttaa ccgcaaagtg 240

actacgcaaa gcttagtaat accttgaaga atttatggct ggtcgttccg ctataacccc 300

tagtagacct ggcagatgag gctagaaata ccccactggt aacagtgttc tagcctgcgt 360

ggctgcctgc acaccctttt tgggtgtgaa gccatatatt tgacaaggtg tgaagagccc 420

cgtgtgctca ctttgagtcc tccggcccct gaatgtggct aaccttaacc ctgcagctag 480

tgtatgtaat ccaacatatg gctagtcgta atgagcaatt gcgggatggg accaactact 540

ttgggtgtcc gtgtttcact ttttcctttt attttgctta tggtgacaat atatataata 600

tatatattgg catc 614

<210> 367

<211> 618

<212> DNA

<213> Human rhinovirus A63 (strain ATCC VR-1173)

<400> 367

ttaaaactgg gtccaggttg ttcccacctg gacctcctaa gtggagtggt acactattat 60

tccggtaatt ttgtacgcca gttttatctc cccacccccc ttttgtaatt tagaagttat 120

gaacacagac caataggtgg caactagcca agttgttaaa ggtcaagcac ttctgtctcc 180

ccggctgacg ttgatatgct ccaacagggc aaaaacaatg aaagtcgtta tccgcaaagt 240

gcctacgcaa agcctagtaa cactttgaaa tgtctatggt tggtcgctca gctattcccc 300

atagtagacc tggcagatga ggctagaaat accccactgg taacagtgtt ctagcctgcg 360

tggctgcctg cgcaccctcc ccgggtgcga agccatatat atgacaaggt gcgaagagcc 420

ccgtgtgctc actttgaatc ctccggctcc tgaatgtggc taaccttaac cccgcagcca 480

gtgcacataa gccaatgtgt ggctggtcgt aatgagcaat tgtgggatgg gaccaactac 540

tttggatgtc cgtgtttcct gtttttcttt tattcttgct tatggtgaca atatatatag 600

tttatatata ttggcatc 618

<210> 368

<211> 614

<212> DNA

<213> Human rhinovirus A64 (strain ATCC VR-1174)

<400> 368

ttaaaactgg atctgggttg ttcccaccca gatcacccaa gtggtgtagt actctgtcat 60

tccggtgact ttgtacgcca gtttttacac cccccccttc ttgtaactta gaagaattaa 120

gttcagacca ataggaaaac gacaaaccag tcgtttaagg tcaagcactt ctgtttcccc 180

ggtcaatgct gatatgcttc accaaggcaa aaacagcagc gatcgttacc cgcaagacgc 240

ctacgcaaag cctagtaatg cctttgaaga tatttgtctg gtcactcagg tacaatccca 300

gtactagacc tggcagatga ggctggaaaa tccccactgg cgacagtgtt ccagcctgcg 360

tggctgcctg cacacccata cgggtgtgaa gccaaatatt ggacaaggtg tgaagagccc 420

cgtgtgctcg tcttgagtcc tccggcccct gaatgcggct aaccttaacc ccacagccat 480

tgcttgcatt ccagcaagtc tatggtcgta atgagcaatt gtgggatggg accgactact 540

ttgggtgtcc gtgtttcact ttttaccttt tattatgctt atggtgacaa tatatagcgt 600

tatatattgg catc 614

<210> 369

<211> 623

<212> DNA

<213> Human rhinovirus A65 (strain ATCC VR-1175)

<400> 369

ttaaaactgg gtgtgggttg cacccaccca cactgtctac ggacgtagtg ctctcatatt 60

acggtatgct tgcacgccag tttgtttccc ctttcccctt ttgtaactat agaagattgc 120

acttaatctc gaccaatagg cagtagtgat ccacactact tacggtcaag cacttctgtt 180

accccggtat gcggagaata ggctctgaaa agggctgaag cttcaattat cgtcatccgc 240

gcaattacta cgcaaagctt agtaacttcc caagaaattc tttggttggc cgttccgctg 300

gtaccccacc agtaggcttg gcagatgagg ctgagtaatc cccactggcg acagtgactc 360

agcctgcgtg gctgcctaca cacctggtaa caggtgtgaa gccatagaaa ggacagggtg 420

tgaagagccc cgtgtgctaa ttgtgattcc tccggcccct gaatgcggct aatctaaccc 480

cacatccaat gcgcataagc caatgcgtat ttggtcgtaa tgggcaactg tgggacggaa 540

ccgactactt tgggtgtccg tgtttctttt tattccttat aattagttca tggtgacaag 600

tataagtgtt tatatttgtc atc 623

<210> 370

<211> 616

<212> DNA

<213> Human rhinovirus A66 (strain ATCC VR-1176)

<400> 370

ttaaaactgg atctaggttg ttcccaccta gatctcccat gtggagtagt actctattat 60

tacggtaatt ttgtacgcca gttttatatc cccttcccct tttgtaactt agaagtcgtg 120

aacacagacc aataggtggc gatcacccag atcgcttaag gtcaagcact tctgtttccc 180

cggtcaacgt tgatatgctc caccagggca aaaacaacgg agatcgttat ccgcaaggtg 240

cctacgcaaa gcctagtaac ctcttggagg atatatggtt ggtcgctcag ctatacccca 300

tagtagacct ggcagatgag gctagaaact ccccactggc gacagtgttc tagcctgcgt 360

ggctgcctgc gcactcttat gagtgcgaag ccatatattt gacaaggtgt gaagagcccc 420

gtgtgctcac cttgagtcct ccggcccctg aatgtggcta accctaaccc tgcagctagt 480

gcatgaaatc catcatgttg ctagtcgtaa tgagcaattg cgggatggga ccaactactt 540

tgggtgtccg tgtttcactt tttcctttta tatttgctta tggtgacaat atatatataa 600

catatatatt ggcatc 616

<210> 371

<211> 611

<212> DNA

<213> Human rhinovirus A67 (strain ATCC VR-1177)

<400> 371

ttaaaactgg atctaggttg ttcccaccta gatctcccaa gtggagtggt acactattat 60

cacggtaatt ttgtacgccg gttttatctc cccttacccc aagtaactta gaagtattac 120

ataaagacca acaggcagtg attatccaga tcactaaagg tcaagcactt ctgtctcccc 180

ggtcaaagtt gatatgctcc aacagggcaa aaacaactga gatcgttatc cgcaaagtgc 240

ctacgcagag cctagtaact accttgaaat tacttggttg gtcgttccac caaaccccat 300

ggtagacctg gcagatgagg ctagaaattc cccactggta acagtgttct agcctgcgtg 360

gctgcctgca caccctttgg gtgtgaagcc aagtaatgga cagggtgtga agagccgagt 420

gtgctcactt tgagtcctcc ggcccctgaa tgtggctaac cttaaccctg cagctagagc 480

gcgcaaacca gcgtgtatct agtcgtaatg agcaattgcg ggacgggacc aactactttg 540

ggtgtccgtg tttcttttta ttcttatatt tgcttatggt gacaatatat aattatttgt 600

atattggcac c 611

<210> 372

<211> 622

<212> DNA

<213> Human rhinovirus A68 (strain ATCC VR-1178)

<400> 372

ttaaaactgg gtccaggttg ttcccacctg gatctcctac gtggagttgt actctattat 60

tccggtaatt ttgtacgcca gttttatctc ccctacccct ctatgtaact tagaagttta 120

tgcacagcgg ccaataggtg acatttatcc aaaatgtcaa aggtcaagca cttctgttac 180

cccggttaga attgatatgc tccaacaggg caaaaacaat tttgatcgtt atccgcaagg 240

caactacgca aagcctagta ccatttttga tgatgtgtgg ttggtcgctc cactgtgaac 300

ccgacagtag acctggcaga tgaggctaga aattccccac tggtaacagt gttctagcct 360

gcgtggctgc ctgcgtaccc ttttgcgggt acgaagccat acatttgaca aggtgtgaag 420

agccccgtgt gcttgccttg agtcctccgg cccctgaatg tggctaacct taaccctgca 480

gctagtgtgc acaatccagt gcatggctag tcgtaacgag caattgcggg atgggaccaa 540

ctactttggg tgtccgtgtt tcttgttttt cttttatatt tgcttatggt gacaatatat 600

acataatata tatattggca cc 622

<210> 373

<211> 625

<212> DNA

<213> Human rhinovirus A71 (strain ATCC VR-1181)

<400> 373

ttaaaactga gtgtgggttg ttcccaccca cactgtctac ggacgtagtg ctctcatatt 60

ccggtatgtt tgcacgccag tttgcctctc cttttcccac gtaacgttag aagcttatgc 120

actaaatcga ccactaggtg agtgataaac catatcattc tttggtcaag cacttctgtt 180

tccccggtat gcagggaata ggccctcccc agggctgaag ccttaattat cgttatccgc 240

acatttacta cgcaaagcct attagcgttc tggaagttca ctggctggtc gttccgctag 300

actaacccgt ctagtagacc tggcagatga ggctggataa accccactgg cgacagtgat 360

ccagcctgcg tggctgcctg cacacctgga aacaggtgtg aagcccatga atggacaggg 420

tgtgaagagc cccgtgtgct aagtgtgagt cctccggccc ctgaatgcgg ctaatctaac 480

cccacagcta atacacacaa accagtgtgg atttagtcgt aatgggtaac tgtgggacgg 540

aaccgactac tttgggtgtc cgtgtttcct tttattcctt ttatattgct tatggtgaca 600

ggtatataga tatatatttg ttacc 625

<210> 374

<211> 610

<212> DNA

<213> Human rhinovirus A74(DQ473494)

<400> 374

ttaaaactgg gtttgggttg atcccaccca aacctcccaa gcggagtggt actctgttat 60

ttcggtaact ttgtacgcca gttttatatt ccctcccccc tttaaactgt aaagctaagc 120

acacagacca atagctgaca atcatccaga ttgttaatgg tcaagcactt ctgtttcccc 180

ggtcaacgtt gatatgctcc aacagggcaa aaacaactgc gatcgttatc cgcaaagtgc 240

ctacgcaaag cctagtagca cctttgaagt catttggttg gtcgctccgc tgttccccac 300

agtagacctg gcagatgagg ctagaaatac cccactggtg acagtgttct agcctgcgtg 360

gctgcctgca caccctttgg gtgtgaagcc aaatcatgga caaggtgtga agagccccgt 420

gtgctcactt tgagtcctcc ggcccctgaa tgtggctaac cttaaccctg cagctagagt 480

gcacaatcca gtgcatttct agtcgtaatg agcaattgcg ggatgggacc aactactttg 540

ggtgtccgtg tttcactttt tacctattat ttgcttatgg tgacaatata tataattata 600

tattggcacc 610

<210> 375

<211> 617

<212> DNA

<213> Human rhinovirus A75 (DQ473510)

<400> 375

ttaaaactgg atccaggttg ttcccacctg gatcacccaa tgggtgtagt atactgttat 60

tacggtaact ttatacgacg gttttatctc cccccccccc ttatgtaact tagaagtttt 120

gcacaccgac caatagtgga caatcagcca gattgtttta ggtcaagcac tactgtttcc 180

cccggtcaac gttgatatgc tccgacaggg caaaaacaac agcgatcgtt atccgcaagg 240

tgcctacaca gagcttagta ccgccttaga aggattgtgg ttggtcgctc agttgcaacc 300

ccagcaatag acctggcaga tgaggctgga cattccccac tggtgacagt ggtccagcct 360

gcgtggctgc ctgcacacct ctatgaggtg tgaagccaga atttcgacaa ggtgcgaaga 420

gccgcgtgtg ctcaccttga atcctccggc ccctgaatgc ggctaacctt aaacccgcag 480

ccatggtcca caatccagtg gatgtatggt cgtaatgagt aattgtggga tgggaccgac 540

tactttgggt gtccgtgttt cctgtttttt cttttatact gcttatggtg acaatatata 600

tgatatatat tggcacc 617

<210> 376

<211> 618

<212> DNA

<213> Human rhinovirus A76 (strain ATCC VR-1186)

<400> 376

ttaaaactgg atccaggttg ttcccacctg gatctcctac aaggagttgt actctattat 60

tgcggtaatt ttgtacgcca gttttatctc ccctccccga tatgtaactt agaagctgaa 120

cacaaagacc aataggtgac atttaatcca aaatgttaaa ggtcaagcac ttctgtttcc 180

ccggccaaag ttgatatgct ccaacagggc aaaaacagct gcggtcgtta tccgcaaaac 240

aactacgcaa agcctagtac caccttctag agcatgtggc tggtcgctcc actataaacc 300

caatagtaga cctggcagat gaggctagga gctcctcact ggtaacagtg tcctagcctg 360

cgtggctgcc tgcgcaccct gacaggtgcg aagccaaatg tttgacaagg tgtgaagagc 420

cccgtgtgct tgttttgagt cctccggccc ctgaatgtgg ctaaccttaa ccctgcagct 480

agtgtgtaca atccagtgca tagctagtcg taatgagcaa ttgcgggatg ggaccaacta 540

ctttgggtgt ccgtgtttct tatttttcct tatatttgct tatggtgaca atgtatatat 600

aacgtatata ttggcatc 618

<210> 377

<211> 613

<212> DNA

<213> Human rhinovirus A77 (strain ATCC VR-1187)

<400> 377

ttaaaactgg atccaggttg ttcccacttg gatcccccaa gaggggtagt actctattat 60

tacggtaatt ttgtacgcca gttttatctc ccttaccccc tttgtaactt agaagattta 120

aacacagacc aataggcgac gatcatccag atcgtttaag gtcaagcact tctgtttccc 180

cggtcaatgc tgatatgctc taccagggca aaaacagtag agatcgttat ccgcaaggtg 240

cctacacaaa gcttagtaac attttgaaga gcatttggtt ggtcgctcag ctattacccc 300

atagtagacc tggcagatga ggctagaagt tccccactgg cgacagtgtt cttgcctgcg 360

tggctgcctg cgcactctca tgagtgcgaa gccatatgtt tgacaaggtg tgaagagccc 420

cgtgtgctca ccttgagtcc tccggcccct gaatgtggct aaccttaacc ctgcagctag 480

tgcatgaaat ccatcatgta actagtcgta atgagcaatt gcgggatggg accaactact 540

ttgggtgtcc gtgtttcact ttttcctttt atatttgctt atggtgacaa tatatatagc 600

atatattgac acc 613

<210> 378

<211> 620

<212> DNA

<213> Human Rhinovirus A78 (strain RvA78/USA/2021/177499)

<400> 378

ttaaaactga atcatggttg ttcccaccat gattacctac gggtgtagta cacttgtatc 60

ccggtacatt tgtgcgccag ttttatatcc cctaccccaa tcgtaactta gaagctgaac 120

aacacaagat caataggcgg tggttatcca aaccatcaat gatcaagcac ttctgtttcc 180

ccggtaggtg tgaatatgct ttaaccaagg cagaagctga tcctatcgtt atccgcaagg 240

tgcctacggg aagcctatta acactttgaa agagatttgg ctggtcgctc agttgcacaa 300

ccccatgcaa tagacctggc agatgaggct agacaaaccc caccagcgat ggtggtctag 360

cctgcgtggc tgcctgcaca ccctttttgg gtgtgaagcc agagattgga cagggtgcga 420

agagcccact gtgctcacct tgagtcctcc ggcccctgaa tgtggctaat cctaaccctg 480

cagctgttgc acaaaagcca ttgtgtatgc agtcgtaatg agcaattgcg ggatggaacc 540

aactactttg ggtgtccgtg tttccttgtt ttctttatta ttgcttatgg tgacaagtat 600

atatagatat atttgtcatc 620

<210> 379

<211> 615

<212> DNA

<213> Human rhinovirus A80 (strain ATCC VR-1190)

<400> 379

ttaaaactra atccaggttg ttcccacctg gatttcccac gaggagcagt actctactat 60

tccggtagtt ttgtacacca gttttatctc ccttccccgt gtaacttaga agacaagcac 120

ttcagaccaa taggatggta actaaccaag ttatcaaagg tcaagcactt ctgtttcccc 180

ggtcaaaatt gatatgctcc accagggcaa aaacagtttt gatcgttatc cgcaaagtgc 240

ctacgcaaag cttagtaaca ctttgaagaa tgtgtggttg gtcgttccgc tattccccat 300

agtagacctg gcagatgagg ctagaaaatc cccactggtg acagtgttct agcctgcgtg 360

gctgcctgcg cacctctttg aggtgcgaag ccattccttt gacaaggtgt gaagagcccc 420

gtgtgctcac tttgagtcct ccggcccctg aatgtggcta accttaaccc tgcagctagt 480

gcatgcaatc cagcatgtct ctagtcgtaa tgagtaattg cgggatggga ccaactactt 540

tgggtgtccg tgtttctctt tttactttta aattgcttat ggtgacaata tatatagtac 600

atatatattg acacc 615

<210> 380

<211> 617

<212> DNA

<213> Human rhinovirus A81 (isolate F06)

<400> 380

ttaaaactgg atctgggttg ttcccaccca gatcacctac atggtgttgt acactattat 60

tacggtaatt ttgtacgcca gttttatatc cccttcccca aatgtaactt agaagcaatg 120

caaactgatc aatagggggc aatcatccag attgctaatg atcaagcact tctgtttccc 180

cggtcaaggt tgatatgctc cacaagggca aaaacaaccg agatcgttat ccacaagatg 240

cctacgcaaa gcctagtaat gcttttgaag atacttggtt ggtcgctcag ctgtgcaccc 300

gacagtagac ctggcagatg aggctagaag tacccctctg gcgacagagt tctagcctgc 360

gtggctgcct gcacaccctt ttgggtgtga agccaagtat tggacaaggt gtgaagagcc 420

ccgtgtgctc atcttgaatc ctccggcccc tgaatgtggc taaccttaaa cctgcagcca 480

gtgcacacaa accagtgtgt agctggtcgt aatgagcaat tgcgggatgg gaccaactac 540

tttgggtgtc cgtgtttcac tttttacctt tatattgctt atggtgacaa tatatatagc 600

acatatatat tgttatc 617

<210> 381

<211> 614

<212> DNA

<213> Human rhinovirus A82 (strain ATCC VR-1192)

<400> 381

ttaaaactgg gagtgggttg ttcccaccca gaccacccac gtggtgttgt gctctgttat 60

tccggtaacc ttgcacgcca attttataac cccctttccc catgtaactt agaagttttg 120

cacataggcc aatagcaagc agcgaaccag ctgtataagg tcaagcactt ctgtttcccc 180

ggtcaatgtt gatatgcttc aacaaggcaa aaacaacagc gatcgttatc cgcaagatgc 240

ctacacaaag cttagtacct cctttgacgg tatttgtctg gtcgctcagg tgtttcccca 300

gcactagacc tggcagatga ggctagaaac tccccactgg tgacagtgtt ctagcctgcg 360

tggctgcctg cacacccttc tgggtgtgaa gccaaatact agacaaggtg tgaagagccc 420

cgtgtgttca tcttgagtcc tccggcccct gaatgcggct aaccttaaac ccgcagccat 480

tactcacaat ccagtgagcg gatggtcgta atgagcaatt gtgggatggg accgactact 540

ttgggtgtcc gtgtttcatg tttttcttta taatttgctt atggtgacaa tatatagtga 600

tatatattgg catc 614

<210> 382

<211> 612

<212> DNA

<213> Human_rhinovirus A85 (strain RvA85/USA/2021/AR424A)

<400> 382

ttaaaactga gtccaggttg ttcccacctg gatttcctac atggagttgt actctgttat 60

tccggtaacc ttgtacgcca gttttatatc cctttccctt ttgtaactta gaagctaagc 120

acacaggcca ataggtggtg attatccaaa tcactaatgg tcaagcactt ctgtttcccc 180

ggtctaaact gatacgctcc aacagggcga aaacagtgca gatcgttatc cgcaaagtgc 240

ctacgcaaag cttagtagca ctttgaagaa tctatggttg gtcgctcagc tataccccct 300

agtagacctg gcagatgagg ctagaaatac cccactggta acagtgttct agcctgcgtg 360

gctgcctgca cacccttacg ggtgtgaagc catatatttg acaaggtgtg aagagccccg 420

tgtgctcact ttgagtcctc cggcccctga atgtggctaa ccttaaccct gcagctagtg 480

catgtaatcc aacatgttgc tagtcgtaat gagtaattgc gggatgggac caactacttt 540

gggtgtccgt gtttcacttt ttacctttta tttgcttatg gtgacaatat atatataata 600

tatattggca tc 612

<210> 383

<211> 621

<212> DNA

<213> Human rhinovirus A88(DQ473504.1)

<400> 383

ttaaaactgg gagtgagttg tccccactca ctctacccac gcggtgttgt acactgttat 60

tacggtaact ttgtatgcca gtttttgttc ccctatcccc ctttaaagta acttagaagc 120

atgaacttac agaccaaaag gtgatgatca cccagatcat taaaggtcaa gcacttctgt 180

ttccccggtc aatgaggata tgctttaccc aaggcaaaaa ccttaaagat cgttatccgc 240

acattaacta cgcaaagcct agtatcgcct ttgatgtatt aagactggtc gttccctgca 300

tacccagcag tagacctggc agatgaagct ggacgttccc cactggcgac agtggtccag 360

cctgcgtggc tgcctgctca cccatttggg tgagaagcct tattattgac aaggtgtgaa 420

gagccccgtg tgcttaatgt gaatcctccg gcccctgaat gtggctaacc ttaaccccgc 480

agccgttact cacaatccag tgaaatgacg gtcgtaacgc gaaagtgtgg gatgggacca 540

actactttgg gtgtccgtgt ttcctatttt ttccttaatg tgtgttatat ggtgacaatt 600

tatagcgtat aagttgtcat c 621

<210> 384

<211> 616

<212> DNA

<213> Human rhinovirus A90 (strain ATCC VR-1291)

<400> 384

ttaaaactgg gttcaggttg ttcccacctg aatctcctac atggagttgt actctattat 60

cccggtaatt ttgtacgcca gttttatcct cccctcccca tgtaacttag aagctgtaca 120

caacgaccaa caggtaacat tcatccagag tgttaaaggt caagcacttc tgtttccccg 180

gtcaaagttg atatgctcca acagggcaaa aacaactgcg atcgttatcc gcagagcaac 240

tacgcaaagc ttagtaccac tttcaatggt gtatggctgg tcgctccact ataaacccaa 300

tagtagacct ggcagatgag gctagaaact ccccactggt aacagtgttc tagcctgcgt 360

ggctgcctgc gtgccctttt gggcacgaag ccttacattt gacaaggtgc gaagagcccc 420

gtgtgcttgc tttgagtcct ccggcccctg aatgtggcta accttaaccc tgcagctagt 480

gtgtgcaatc cagcacatgg ctagtcgtaa tgagcaattg cgggatggga ccaactactt 540

tgggtgtccg tgtttcttgt ttttctttta tatttgctta tggtgacaat atatatagaa 600

tatatatatt ggcatc 616

<210> 385

<211> 615

<212> DNA

<213> Human rhinovirus A94 (strain ATCC VR-1295)

<400> 385

ttaaaactgg atctgggttg ttcccaccca gatcacccaa gaggtgtagt gctctgtcat 60

tccggtgacc ttgcacgcca gttttatcac cccttccctc ttgtaactta gaagaattaa 120

actcagacca ataggaagcg acaagccagt cgtgacaggt caagcacttc tgtttccccg 180

gtcaaagttg atatgcttca ccaaggcaaa aacaacagcg atcgttatcc gcaagatgcc 240

tacgcaaagc ctagtagtgc ctttgaaaat atttgtctgg ttgctcaagt acaaccccag 300

tactaaacct ggcagatgag gctggagctt ccccactggt aacagtgttc cagcctgcgt 360

ggctgcctgc acacccttac gggtgtgaag ccaaatattg gacaaggtgt gaagagcccc 420

gtgtgctcat cttgagtcct ccggcccctg aatgcggcta accttaaccc cacagccatt 480

gctcacaatc cagtgagtgt atggtcgtaa cgagcaattg tgggatggga ccgactactt 540

tgggtgtccg tgtttctctt ttttcctttt taattctgct tatggtgaca atatatagtg 600

ttatatattg gcatc 615

<210> 386

<211> 608

<212> DNA

<213> Human rhinovirus A95 (strain ATCC VR-1301)

<400> 386

ttaaaactga gttcaggttg ttcccatctg aacttcccac gtggagtagt acactattat 60

tacggtaatt ttgtacgcca gttttgcctc cctccccaaa tgtaacttag aagatgtaca 120

caacggccaa caggcggtgg ttatccaaac catcaaaggt caagcacttc tgtttccccg 180

gtcaatgttg atatgctcca acagggcaaa aacaactgcg atcgttatcc gcaagatgcc 240

tacgcaaagc ttagtaacac tttggaagat ttatggctgg tcgttccact ataccccata 300

gtagacctgg cagatgaggc tagaaatacc ccactggtga cagtgttcta gcctgcgtgg 360

ctgcctgcgc accctatggg tgcgaagcca tacattggac aaggtgtgaa gagccccgtg 420

tgctcatctt gagtcctccg gcccctgaat gtggctaacc ttaaccccgc agccaatgca 480

cacaatccag tgtgtttttg gtcgtaatga gtaattgtgg gatgggacca actactttgg 540

gtgtccgtgt ttcacttttt cctttattat gcttatggtg acaatatata tacatatata 600

ttgacacc 608

<210> 387

<211> 617

<212> DNA

<213> Human rhinovirus A96 (strain ATCC VR-1296)

<400> 387

ttaaaactgg gtctgggttg tccccaccta gatcacccaa gtggtgtagt acactgttat 60

ttcggtaacc ttgtacgcca gttttataac cccctcccca aatgtaactt agaagctgta 120

cacaacgacc aatagagggt aatcatccag attacttacg gtcaagcact tctgtctccc 180

cggtttactt tgatatgctc caacagggca aaaacaaagt gaatcgttac ccgcaagatg 240

cctacgcaac gcctagtaac atcctgaatt gtttatggtt ggtcgctcaa ttacaacccc 300

agtaatagac ctggcagatg aggctagata ttccccactg gcgacagtga tctagcctgc 360

gtggctgcct gcacacccct tgtgggtgtg aagccataat catgacaggg tgcgaagagc 420

cacgtgtgct catcttgagt cctccggccc ctgaatgcgg ctaaccctaa ccctgcagcc 480

attgcctaca aaccagtggg tatgtggtcg taacgagaaa ttgcgggatg ggaccgacta 540

ctttgggtgt ccgtgtttca ctttttacct tatatttgct tatggtgaca atatatacga 600

gtttatatat tgacacc 617

<210> 388

<211> 607

<212> DNA

<213> Human rhinovirus A98 (strain RvA98/USA/2021/W58KP8)

<400> 388

aaactgggtc taggttgttc ccacctagat ctcctacatg gagttgtact ctattattcc 60

ggtaattttg tacgccagtt ttatatccct ttccccatgt aacttagaag tttgacacaa 120

tgaccaatag gcggcaatta accaaattgc taaaggtcaa gcacttctgt ttccccggtc 180

aagattgata cgctctacca gggcgaaaac agttgagatc gttatccgca aagtgcctac 240

gcgaagctta gtaacatctt gaagaattta tgactggtcg ctccgccatt acccatggta 300

gacctggcag atgaggctag aaactcccca ctggtaacag tgttctagcc tgcgtggctg 360

cctgcacacc cttacgggtg tgaagccata tatttgacaa ggtgtgaaga gcctagtgtg 420

ctcactttga gtcctccggc ccctgaatgt ggctaacctt aaacccgtag ctagtgcaca 480

caggccagtg tgtagctagt cgtaacgagc aattacggga tgggaccaac tactttgggt 540

gtccgtgttt cactttttac ctttatattt gcttatggtg acaatatata gattatatat 600

tgacacc 607

<210> 389

<211> 611

<212> DNA

<213> Human rhinovirus A100 (strain ATCC VR-1300)

<400> 389

ttaaaactga atccagattg ttcccatctg gatttcctac atggagttgt actctattat 60

tccggtaatt ttgtacgcca gttttatcac cccttccccc gtaacttaga agtttgaaac 120

aaaagaccaa taggaggtaa ctatccaagt tactataggt caagcacttc tgtttccccg 180

gtcaaagttg atatgctcca ccagggcaaa aacaattgag atcgttatcc gcaaagtgcc 240

tacgcaaagc ctagtagtat cttgaaaagc atgtggttgg ttgctccgct gtaccccaca 300

gtaaacctgg cagatgaggc tagaagttcc ccactggtga cagtgttcta gcctgcgtgg 360

ctgcctgcgc actctttgag tgcgaagcca tatgtttgac aaggtgtgaa gagccccgtg 420

tgctcacttt gagtcctccg gcccctgaat gtggctaacc ttaaccctgc agctagtgca 480

tgcaatccag catgtggcta gtcgtaatga gcaattgcgg gatgggacca actactttgg 540

gtgtccgtgt ttcacttttt tccttttata attgcttatg gtgacaatat atagtgatat 600

atattgacac c 611

<210> 390

<211> 615

<212> DNA

<213> Human rhinovirus A101 (strain SC1124)

<400> 390

ttaaaactga gtcatggttg ttcccaccat gattacccac gggtgtagta tacttgtatc 60

ccggtacatt tatacgccag ttttataatc cccaccccaa atgtaactta gaagcttgta 120

cacaatgatc aataggatga tggctatcca agtcatctaa gatcaagcac ttctgtctcc 180

ccggtgagtg tgaatatgct ttacccaagg caaaagctaa tctcatcgtt atccgcaagg 240

tgcctacggg aagcctagta atactctgaa gatattttgg ttggtcgatc agttgcactc 300

acccatgcaa tagacctggc agatggggct aggcacacct cactggtaac agtggcctag 360

cccgcgtggc tgcctgcaca ccttcgggtg tgaagccata atattgacag ggtgtgaaga 420

gcctactgtg ctcaccttga gtcctccggc ccctgaatgt ggctaatcct aaccccgtat 480

ccattgcaca caaaccagtg tgtctatggt cgtaatgggt aactacggga tggaaccaac 540

tactttgggt gtccgtgttt cttgtttttc tttaatactg cttatggtga cattatatat 600

tgatatagtg tcatc 615

<210> 391

<211> 610

<212> DNA

<213> Human rhinovirus A103 (strain MCL-18-H-1122)

<400> 391

tgggttgttc ccacccacac tgtctacgga cgtagtactc tcatatcacg gtatgtttgt 60

acgccagttt gccctccccc ccccaaatgt aacttagaag tttgtacttt ataaaggcca 120

gtaggcgagt agtaaaccat actactcatg gtcaagcact tctgtttccc cggtatgcgg 180

agaataagct ttaacaaagg ctgaagcttc aattatcgtt atccgcatga taactacgca 240

aagcttagta acattttgaa agtttattgg ttggtcgctc cactggaaaa cccccccagt 300

agacctggca gatgaggcta agtaatcccc actggtgaca gtgacttagc ctgcgtggct 360

gcctgcacac ctggaaacag gtgtgaagcc aagtaatgga caaggtgtga agagccccgt 420

gtgcttatca tgagtcctcc ggcccctgaa tgcggctaat ccaaccccac agctattgca 480

cataatccaa tgtgtattta gtcgtaacgg gcaactgtgg gacggaaccg actactttgg 540

gtgtccgtgt ttcctttatt ctcatttaag tttcctatgg tgacaaatat aagtgattat 600

atctgtcatc 610

<210> 392

<211> 626

<212> DNA

<213> Human rhinovirus B3 (NC_038312.1)

<400> 392

ttaaaacagc ggatgggtac cccaccatcc gacccactgg gtgtagtact ctggtacttc 60

gtacctttgt acgcctgttc ttcccattgt acccttcctg aacttccaac ccaagtaacg 120

ttagaagctc aacatttagt acaacaggaa gcaccacatc cagtggtgtt tagtacaagc 180

acttctgttt ccccggagcg aggtataggc tgtacccact gccaaaaacc tttaaccgtt 240

atccgccaac caactacgta aaagctagta gtattatgtt tttaactagg cgttcgatca 300

ggtggatttc ccctccacta gtttggtcga tgaggctagg aattccccac gggtgaccgt 360

gtcctagcct gcgtggcggc caacccagct tatgctggga cgccttttta tagacatggt 420

gtgaagactc gcatgtgctt ggttgtgatt cctccggccc ctgaatgcgg ctaaccttaa 480

ccctggagcc ttgtgtcaca aaccagtgat gataaggtcg taatgagcaa ttccgggacg 540

ggaccgacta ctttgggtgt ccgtgtttct tatttttctt attattgtct tatggtcaca 600

gcatatatat aacatatact gtgatc 626

<210> 393

<211> 625

<212> DNA

<213> Human rhinovirus B4 (DQ473490.1)

<400> 393

ttaaaacagc ggatgggttt cccaccatcc gacccactgg gtgtagtgct ctggtatttt 60

gtacctttgc acgcctgttt cccatttgta cccttcctta atctccttcc cccgtaacgt 120

tagaagtttt ggaattttaa agtacaatag gaagcgccac atccagtggt gttgcgtaca 180

aatacttctg tttacccgga gcgaggtata ggttgtaccc acggccaaaa gcctttaacc 240

gttatccgcc aatcaactac gtaacggcta gtatcatctt gcttttgatt tggtgttcga 300

tcaggtggta tcccccacta gtctggtcga tgaggctagg aattccccac gggcgaccgt 360

gtcctagcct gcgtggcggc cagcccagct tttgctggga cgccttttca aagacatggt 420

gtgaagacct gcatgtgctt ggttgtgagt cctccggccc ctgaatgcgg ctaaccttaa 480

ccctggagcc cagcagcata atccaatgtt gtttgggtcg taatgagcaa ttccgggacg 540

ggaccgacta ctttgggtgt ccgtgtttcc ttttattctt acattgtctt atggtcacag 600

catatatatt atatatactg tgatc 625

<210> 394

<211> 631

<212> DNA

<213> Human rhinovirus B5 (strain ATCC VR-485)

<400> 394

ttaaaacagc gggtgggtat cccaccaccc gacccactgg gtgtggtaca ctggtacttt 60

gtacctttgt atgcctgttt gcttccactc ccctttacct aatattcttc cccaagtata 120

ttttgcggta acgttagaag aagcaaagtt agtgcaatag gatgtatcac aaccagtggt 180

ataaagcaca agcaattctt gttccccgga gctggatata gactgctaac gtggtcaaaa 240

gtccttaacc gttatccgcc agctaactgc gagaagacta gtaacaccat gtttgttttt 300

agacgttcga tcaggtgaat accccattca ctagtttggt cgatgaggct gagaataccc 360

cacaggtaac tgtgtctcag cctgcgtggc ggccaacccg actcatgtcg ggacgcctac 420

taatagacat ggtgtgaaga tcctattgcg cttagttgtg agtcctccgg cccctgaatg 480

cggctaaccc taaccccgga gccttgtggt gtaaaccaac atttgcaagg tcgtaatgag 540

taattctggg atgggaccga ctactttggg tgtccgtgtt tcctttattt ttattgtgtg 600

tgtcttatgg ttacaaatac aatttgtaat c 631

<210> 395

<211> 627

<212> DNA

<213> Human rhinovirus B6 (DQ473486.1)

<400> 395

ttaatacagc ggatgggtat cccaccatcc gacccactgg gtgtagtact ctggtattat 60

gtacctttgt acgcctgttc ttcccattgt acccttccca aatttccaac ccaagtaacg 120

ttagaagttt gacattaaag tacaatagga ggcaccacat ccagtggtgt tttgtacaag 180

cacttctgtt tccccggagc gaggtatagg ctgtacccac tgccaaaaac ctttaaccgt 240

tacccgccag ccaactacgt aaaagctagt aacatcttgt ttgtaacttg gcgttcgatc 300

aggtggattc cccctccact agtttggtcg atgaggctag gaactcccca cgggtgaccg 360

tgtcctagcc tgcgtggcgg ccaacccagc tcttgctggg acgccctttt atagacatgg 420

tgtgaagact cgcatgtgct tggttgtgat tcctccggcc cctgaatgcg gctaacccta 480

accctggagc cttgtgttac aaaccagtaa tattaaggtc gtaatgagca attccgggac 540

gggaccgact actttgggtg tccgtgtttc tcatttttct tattattgtc ttatggtcac 600

agcatatttg ttaaatatac tgtgatc 627

<210> 396

<211> 618

<212> DNA

<213> Human rhinovirus B17 (EF173420)

<400> 396

tttaaacagc ggatgggttc cccaccatcc gacccactgg gtgtagtact ctggtatttt 60

gtacctttgt acgcctgttt ctcccctacc tcccaaccta aacaatcctg gtaacttaga 120

agacttaaat catcgtacaa taggtgctgt cacatccagt gacggctagt acaagcactt 180

ctgtttcccc ggagcggagt ataaatggcc accgctgtca aaagctctta accgttatcc 240

gccaattaac tacgcaacgg ctagtaacat cttgttattt ttagggcgtt cgatcaggtg 300

agtaaacccc tcactagtct ggtcgatgag gctgagaatt ccccacgggc gaccgtgtct 360

cagcctgcgt ggcggccagc ccagctaatg ctgggacgcc ttaattgtga catggtgtga 420

agacccacgt gtgcttaatt gtgagtcctc cggcccctga atgcggctaa cctaaaccct 480

ggagccttga gacacaatcc agtgttggca aggtcgtaat gagtaattcc gggacgggac 540

cgactacttt gggtgtccgt gtttcctttt attttcaaat tgttcttatg gtcacaatat 600

aagtaatata ttgtgatc 618

<210> 397

<211> 632

<212> DNA

<213> Human rhinovirus B26 (strain ATCC VR-1136)

<400> 397

ttaaaacagc gggtgggtat cccaccaccc gacccactgg gtgttgtaca ctggtatttt 60

gtacctttgt atgcctgttt gcacctcccc accccttcca attaccctta cccgaattrt 120

attatgcggt aacattagaa gaagtgaaca cagtgcaata ggacgtatca catccagtga 180

tataaagcac aagcaattct tgttccccgg agctggatat agactgctaa cgtggttgaa 240

ggtccttaac cgttatccgc caaccaacta cgagacggct agtaatatca tgtttgtctt 300

tgagcgttcg atcaggtgaa ttccccattc actagtttgg tcgatgaggc tgagaactcc 360

ccacaggtga ctgtgtctca gcctgcgtgg cggccaaccc agccacggct gggacgccca 420

ctgatagaca tggtgtgaag acccaattgt gcttggttgt gactcctccg gcccctgaat 480

gcggctaacc tcaaccccgg agccttgtag tgtaagccaa cacatacaag gtcgtaatgg 540

gcaactctgg gacgggaccg actactttgg gtgtccgtgt ttcctttatt tttatctttt 600

gtgtcttatg gttacaagta ttgattgtaa cc 632

<210> 398

<211> 622

<212> DNA

<213> Human rhinovirus B35 (strain ATCC VR-1145)

<400> 398

ttaaaacagc ggatgggttc cctaccatcc gacccactgg gtgtagtact ctggtactct 60

gtacctttgt acgcctgttt atccactacc cttttcctaa attttccacc cgtgtaacct 120

tagaagcaaa caaattgtac aatagggtgc atcacaacca gtgatgtaca gtacaagcac 180

ttctgtttcc ccggagcggg gtatagacgg tcaccactgt caaaagccct taaccgttac 240

ccgccaacca actacgtaac ggctagtaac accatgaatt tgtcttgacg ttcgatcagg 300

tggtaacccc ccactagttt ggtcgatgag gctgagaact ccccacgggt aaccgtgtct 360

cagcctgcgt ggcggccaac ccagcttctg ctgggacgcc aattcattga catggtgtga 420

agacctgcat gtgcttggtt gtgattcctc cggcccctga atgcggctaa ccctaacccc 480

ggagccacgc ggcataatcc aatgtcgtat gggtcgtaat gagtaattct gggatgggac 540

cgactacttt gggtgtccgt gtttcctgtt tttccaaatt attcttatgg tcacagcata 600

taaatagata tatactgtga tc 622

<210> 399

<211> 630

<212> DNA

<213> Human rhinovirus B37(EF173423)

<400> 399

tttaaacagc ggatgggtat cccaccatcc gacccactgg gtgtagtact ctggtatttt 60

gtacctttgt acgcctgttg ttcctaatgt acccacccta aaacttccta cccaagtaac 120

gttagaagtt tcatcaacaa gtacaatagg aagcatcaca tccagtggtg ttttgtacaa 180

gcacttctgt ttccccggag cgaggtatag gctgtaccca ctgccgaaag cctttaaccg 240

ttatccgcca accaactacg taaaagctag tatcatcatg ttttaaaata ggcgttcgat 300

caggtggatc ccccctccac tagtttggtc gatgaggcta ggaactcccc acgggtgacc 360

gtgtcctagc ctgcgtggcg gccaacccag cttctgctgg gacgcctttt tatggacatg 420

gtgtgaagac tcgcatgtgc ttggttgtga ctcctccggc ccctgaatgc ggctaacctt 480

aaccccggag ccctgtgttg caatccagta acattagggt cgtaatgagc aatttcggga 540

cgggaccgac tactttgggt gtccgtgttt ctcatttttc ttattattgt cttatggtca 600

cagcatatat ataacgtata tactgtgatc 630

<210> 400

<211> 632

<212> DNA

<213> Human rhinovirus B42 (strain ATCC VR-338)

<400> 400

ttaaaacagc gggtgggtat cccaccaccc gacccactgg gtgtggtaca ctggtacttt 60

gtacctttgt atgcctgttt gcatcccctt ccctttacct aacatccctc cccaagttat 120

attttgcggt aacgttagaa gaagtgaacy tagtgcaaca ggaagtatca ccaccagtga 180

tataacgcac aagcaattct tgttccccgg agctggatat agactgctaa cgtggttgaa 240

agtccttaac cgttatccgc cagttaactg cgaaaagact agtaacatca tgcacatttc 300

taggtgttcg atcaggtgaa aaccccattc actagtttgg tcgatgaggc tgagagtacc 360

ccacaggcga ctgtgtctca gcctgcgtgg cggccaaccc gacttatgtc gggacgccta 420

ttaatagaca tggtgtgaag atcctattgc gcttagctgt gagtcctccg gcccctgaat 480

gcggctaacc ttaaccccgg agtcttgtga tgtaagccaa catttgcaag gtcgtaatga 540

gtaattctgg gatgggaccg actactttgg gtgtccgtgt ttcctttatt cttatcattt 600

gtgtttcatg gttacaagca ttgtttgtaa tc 632

<210> 401

<211> 622

<212> DNA

<213> Human rhinovirus B48 (DQ473488)

<400> 401

ttaaaacagc ggatgggtat cccaccatcc gacccactgg gtgtagtact ctggtactct 60

gtacctttgt acgcctgttt ctcctcaacc ctcccctacc cgttatatat ctcagtaact 120

tagaagatat cattaccgca caatagggac cgttgtaacc agcaacggtc agtgcaagca 180

cttctgtttc cccggagcgg agtatagacg gccactgctg tcaaaagctc ttaaccgtta 240

cccgccagtt aactacacaa cagctagtaa catcttgttg ttcttagggc gttcgatcag 300

gtgagtttac cccctcacta gtctggtcga tgaggctgag aaatccccac gggtgaccgt 360

gtctcagcct gcgtggcggc cagcccagct tatgctggga cgccttaagt atgacatggt 420

gtgaagactc gcgtgtgctt agctgtgagt cctccggccc ctgaatgcgg ctaacctaaa 480

ccccggagcc ttgaggcaca atccagtgcc cgcgaggtcg taacgagaaa ttctgggacg 540

ggaccgacta ctttgggtgt ccgtgtttcc ttttatcttt ttgatgttct tatggttaca 600

atatataaag tatattgtaa tc 622

<210> 402

<211> 624

<212> DNA

<213> Human rhinovirus B52 (isolate F10)

<400> 402

ttaaaacagc ggatgggtat cccaccatcc gacccactgg gtgtagtacy ctggtattat 60

gtacctttgt acgcctgttt ctccctccct actaccccgc ctcaagcatc ctgtaactta 120

gaagaattga aaaaccgcac aacaggagct attacaacca gtaatagtta gtgcaagcac 180

ttctgtttcc ccggagcgga gtatagacgg ccaccgctgt caaaagctct taaccgttat 240

ccgccagtta actacgtaac ggctagtaac atcttgttgt gcttagggtg ttcgatcagg 300

tgaagaaaaa cccccttcac tagtctggtc gatgaggctg agaagtcccc acgggcgacc 360

gtgtctcagc ctgcgtggcg gccagcccag catatgctgg gacgccttaa gtatgacatg 420

gtgtgaagac ccgcatgtgc ttaactgtga gtcctccggc ccctgaatgc ggctaaccta 480

aaccctggag ccttggagca caagccagtg cttacaaggt cgtaatgagc aattccggga 540

cgggaccgac tactttgggt gtccgtgttt ccttttatct cattattgtt cttatggtta 600

caatatatag agtatattgt aatc 624

<210> 403

<211> 620

<212> DNA

<213> Human rhinovirus B69 (strain ATCC VR-1179)

<400> 403

ttaaaacagc ggatgggtat cccaccatcc gacccactgg gtgtagtact ctggtacttt 60

gtacctttgt acgcctgttt ctccctccct tcccaaccta aacaatcctt gtaacttaga 120

aggtttcgat tatcgcacaa tagggactat tgcaaccagc aatagtcagt gcaagcactt 180

ctgtttcccc ggagcggagt gtagatggcc accgctgtca aaagctctta accgttatcc 240

gccagttaac tacgcaacgg ctagtagtgc cttgttgctt ttatggcgtt cgatcaggcg 300

agatttaccc cctcgctagt ctggtcgatg aggctgagaa ttccccacgg gcgaccgtgt 360

ctcagcctgc gtggcggcca gcccagcctt tgctgggacg ccttgattgc gacatggtgt 420

gaagacccgc gtgtgcttaa ctgtgagtcc tccggcccct gaatgcggct aacctaaacc 480

ctggagcctt gaaacacaac ccagtgttcg caaggtcgta acgagcaatt ccgggacggg 540

accgactact ttgggtgtcc gtgtttcctt tattctttca attgttctta tggtcacaat 600

ataagtgtta tattgtgatc 620

<210> 404

<211> 622

<212> DNA

<213> Human rhinovirus B70 (DQ473489)

<400> 404

ttaaaacagc tgatgggttc cccaccatcc gacccaatgg gtgtagtact ctggtattct 60

gtacctttgt acgcctgttt ctccccaccc ccccgtccta aataatccca gtaacttaga 120

agatttgaat tatcgtacaa taggtgctgt cacatccagt gatggctagt acaagcactt 180

ctgtttcccc ggagcggagt atagatggcc accgctgtca aaagctctta accgttatcc 240

gccaattaac tacgcaacgg ttagtaacat cttgtcattt ttgaggcgtt cgatcaggtg 300

agtacacccc tcactagtct ggtcgatgag gctgagaatt ccccacgggc gaccgtgtct 360

cagcctgcgt ggcggccagc ccagcttatg ctgggacgcc ttaattgtga catggtgcga 420

agacccacgt gtgcttaatt gtgagtcctc cggcccccga atgcggctaa cctaaacccc 480

ggagccttga gacacaatcc agtgttagca aggtcgtaac gagcaattct gggacgggac 540

cgactacttt gggtgtccgt gtttcctgtt attctcaaat tgttcttatg gtcacaatat 600

aagtaatata tatattgtga tc 622

<210> 405

<211> 630

<212> DNA

<213> Human rhinovirus B72 (strain ATCC VR-1182)

<400> 405

ttaaaacagc ggatgggttc tcccaccatc cgacccactg ggtgtagtac tctggtatga 60

tgtacctttg tacgcctgtt tattcccaaa cccccacccg ttattcgccc aacccctgta 120

acgttagaag tttggaactt aattgtacaa tagggagcac catatccaat ggtgtaacgt 180

acaagcactt ctgtttcccc ggagcgaggt ataggtggta cccactgccg aaagccttta 240

accgtcatcc gccaatcaac tacgtaacag ctagtaacat cttgttgttg attggacgtt 300

cgatcaggtg gttcaccccc cactagtttg gtcgatgagg ctgggaatcc cccacgggtg 360

accgtgtccc agcctgcgtg gcggccaacc cagcttatgc tgggacgccc tttcaatgac 420

atggtgtgaa gactcgcatg tgcttgattg tgagtccgcc ggcccctgaa tgcggctaac 480

cctaaccctg gagccttgca ccacaatcca gtggtgtctg ggtcgtaatg agtaattctg 540

ggatgggacc gactactttg ggtgtccgtg tttctcattt ttcttttaat tgtcttatgg 600

tcacagcata tagtattata tactgtgatc 630

<210> 406

<211> 624

<212> DNA

<213> Human rhinovirus B79 (isolate ZB/CHN/18)

<400> 406

ttaaaacagc ggatgggtat cccaccatcc ggctcactga gcgtagtact ctggtataat 60

gtacctttgt acgcctgttt tcccaaaatt ccccatcctt tacatttcct accccgtaac 120

ataagaagct ttcaaacagt acaataggaa gcaccacatc cagtggtgtt atgtacaagc 180

acttctgttt ccccggagcg aggtataggc ggtcaccact gccaaaagcc tttaaccgtt 240

atccgccaac caactacgta atggctagta acaccattga tttaaattga cgttcgatca 300

ggtggtatcc ccccactagt ttggtcgatg aggctaggaa taccccacgg gtgaccgtgt 360

cctagcctgc gtggcggcca acccagcgta tgctgggacg ccagtttaat gacatggtgc 420

gaagacttgc atgtgcttgg ttgagactcc tccggcccct gaatgcggct aaccttaacc 480

ccggagcctt gcagcataat ccaatgttgt tgaggtcgta atgagcaatt ctgggatggg 540

accgactact ttgggtgtcc gtgtttcctg tttttcttta cattgtctta tggtcacagc 600

atatatattg tatatactgt gatc 624

<210> 407

<211> 626

<212> DNA

<213> Human rhinovirus B83 (strain ATCC VR-1193)

<400> 407

ttaaaacagc ggatgggtat cccaccatcc gacccactgg gtgtagtact ctggtatttt 60

gtacctttgt acgcctgttt cttccctttg tacccgcccc ttaaacttcc accccacgta 120

acgttagaag tttatacaag cagtacaata ggttgcacca taaccagtgg tgtttcgtac 180

aagcacttct gtttccccgg agcggggtat agacggtacc cactgtcaaa agcccttaac 240

cgttatccgc caatcaacta cgtaatggct agtaacacca tgaaggtaag ttgacgttcg 300

atcaggtggt aacccccact agtttggtcg atgaggctag gaattcccca cgggtaaccg 360

tgtcctagcc tgcgtggcgg ccaacccagc atttgctggg acgccaattt actgacatgg 420

tgtgaagact tgcatgtgct tgattgtgac tcctccggcc cctgaatgcg gctaacctta 480

accccggagc cttgcagcat aatccaatgt tgtggaggtc gtaatgagca attctgggat 540

gggaccgact actttgggtg tccgtgtttc ctgttttctt taagttgtct tatggtcaca 600

gcatatatac tatatatact gtgatc 626

<210> 408

<211> 621

<212> DNA

<213> Human rhinovirus B84 (strain ATCC VR-1194)

<400> 408

ttaaaacagc ggatgggtat cccaccatcc ggcccactgg gcgtagtaca ctggtattct 60

gtacctttgt acgcctgttt gccgcccctc ccccttttat taccacattt gtggtcgctg 120

caacgttaga agattttaaa gtagtgcaat aggaagcatc acaaccagtg gtgttatgca 180

caaacacttc tgtttccccg gagcgaggta tagacggtcc ccactgtcaa aagcctttaa 240

ccgttatccg ccaaccaact acgagacagc tagtaatgcc ctgttctcct gcaaggcgtt 300

cgatcagaca gatttcccct ctgttagtct ggtcgatgag gctagaagta ccccacaggt 360

aactgtgttc tagcctgcgt ggcggccagc ccagcttctg ctgggacgcc tttcaagaga 420

catggtgtga agactcaatt gtgcttggtt gtgagtcctc cggcccctga atgcggctaa 480

ccttaaaccc ggatccatgc tatgcaaacc agcatagtta tggtcgtaat gggcaactct 540

gggatgggac cgactacttt gggtgtccgt gtttccattt ttactttttg tgtcatttat 600

ggttacaatt aagttgtaac c 621

<210> 409

<211> 631

<212> DNA

<213> Human rhinovirus B86 (strain ATCC VR-1196)

<400> 409

ttaaaacagc ggatgggtac cctaccatcc gacccactgg gtgtagtaca ctggtacatt 60

gtacctttgt acgcctgttt ctccctattg taccttccct cacaatccta acccctgtaa 120

cgttagaaga tgtgcaactc gtgcaatagg agatactgca accagtggta tcaagcacaa 180

gaacttctgt ttcctcggag cggagtatag atggtcccca ccgtcaaaag ctcttaaccg 240

ttatccgcca atcaactacg tgacgactgg tagcaycatt ttgacacttt gacgttcgat 300

caggtaggct taccccacct actagtttgg ccgatgaggc tgggagtccc tcacaggtaa 360

ctgtgtccca gcctgcgtgg cggccaaccc agcaattgct gggacgccaa tttgttgaca 420

tggtgtgaag atcttaatgt gcttggttgt gagtcctccg gcccctgaat gcggctaacc 480

ttaaccccgg agccttgtgt cacaagccag tgacattaag gtcgtaatga gtaatttcgg 540

gatgggaccg actactttgg gtgtccgtgt ttcctgtttt tctttatgat tgtcttatgg 600

tcacagcata tagtaattat atactgtgat c 631

<210> 410

<211> 598

<212> DNA

<213> Human rhinovirus B91 (strain RvB91/USA/2021/95333)

<400> 410

cccaccatcc gacccactgg gtgtagtact ctggtacttt gtacctttgt acgcctgttt 60

ctccccccct cccaacccac acaatcctgg taacttagaa gttttgaact attgtacaac 120

aggagctgcc acatccagtg gtggttagta caagcacttc tgtttccccg gggcggagta 180

tagacggcca ccgctgtcaa aagctcttaa ccgttatccg ccaattaact acgcaacggc 240

tagtaatgtc atgttgctat tagggcgttc gatcaggtga gtgctcccct cactagtctg 300

gtcgatgagg ctgagaattc cccacgggtg accgtgtctc agcctgcgtg gcggccagcc 360

cagcttatgc tgggacgcct taattgtgac atggtgtgaa gacccacgtg tgcttaattg 420

tgagtcctcc ggcccctgaa tgcggctaac ctaaaccctg gagccttgag acacaatcca 480

gtgttggcaa ggtcgtaatg agcaattccg ggacgggacc gactactttg ggtgtccgtg 540

tttcctttta tttccaaatt gttcttatgg tcacaatata agtgtacata ttgtgatc 598

<210> 411

<211> 627

<212> DNA

<213> Human rhinovirus B92 (strain ATCC VR-1293)

<400> 411

ttaaaacagc ggatgggtat cccaccatcc ggcccactgg gtgtagtact ctggtacatt 60

gtacctttgt acgcctgttt tccccctctt gtacccgccc ttcaagctcc ttgcccaagt 120

aacgttagaa gtttgaacat tggtacaata ggaagcatca catccagtgg tgtactgtac 180

aaacacttct gttgccccgg agcgaggtat agatggtccc caccgtcaaa agcctttaac 240

cgttatccgc caatcaacta cgtaatggct agtagcacct tggatttaag ttggcgttcg 300

atcaggtggt aacccccact agtttggtcg atgaggctag gaattcccca cgggtgaccg 360

tgtcctagcc tgcgtggcgg ccaacccagc atccgctggg acgccaattt aatgacatgg 420

tgtgaagacc tgcatgtgct tgattgtgag tcctccggcc cctgaatgcg gctaacccta 480

accccggagc cttgcagcac aatccagtgt tgttaaggtc gtaatgagca attctgggat 540

gggaccgact actttgggtg tccgtgtttc ttatttttct tgaatttttc ttatggtcac 600

agcatatata cattatatac tgtgatc 627

<210> 412

<211> 628

<212> DNA

<213> Human rhinovirus B93 (EF173425)

<400> 412

ttaaaacagt ggatgggttt cctaccatcc gacccactgg gtgtagtgct ctggtatatt 60

gtacctttgc acgcctgttt tcccattgta cccttcctta aattcctccc catgtaacgt 120

tagaagttta agaacataaa tgtacaatag gaagcatcac atccagtggt gtatggtaca 180

agcacttctg tttatccgga gcgaggtata agcggtaccc actgctgaaa gcctttaacc 240

gttaaccgcc aatcaactac gtaacggcta gtattacctt gtttgcaatc tggcgttcga 300

tcaggtggtt ccccccacta gtttggtcga tgaggctagg agttccccac gggcgaccgt 360

gtcctagcct gcgtggcggc caacccagca tttgctggga cgcctttttg tagacatggt 420

gtgaagacct gcatgtgctt gattgtgagt cctccggccc ctgaatgcgg ctaaccttaa 480

ccccggagcc ttgtgacata atccaatgtc attaaggtcg taatgagcaa ttctgggacg 540

ggaccgacta ctttgggtgt ccgtgtttct ctttattctt tacattgtct tatggtcaca 600

gcatatataa tacatatata ctgtgatc 628

<210> 413

<211> 625

<212> DNA

<213> Human rhinovirus B97 (strain ATCC VR-1297)

<400> 413

ttaaaacagc ggatgggttt cccaccatcc gacccactgg gtgtagtgct ctggtatttt 60

gtacctttgc acgcctgttt cccctttgta cccatcctga atttcctccc tctgcaacgt 120

tagaagtttg tgaaattaaa agtacaatag gaagcatcac atccagtggt gttcagtaca 180

agcactcctg tttccccgga gcgaggtata ggttgtaccc acgaccgaaa gcctttaacc 240

gttatccgcc aatcaactac ataacggcta gtatcatcat gtttttgatc tggcgttcga 300

tcaggtggtt tcccccacta gtctggtcga tgaggctagg atttccccac gggcgaccgt 360

gtcctagcct gcgtggcggc cagcccagct tatgctggga cgccttttta aagacatggt 420

gtgaagacct gcatgtgctt gattgtgagt cctccggccc ctgaatgcgg ctaaccttaa 480

ccctggagcc cgacagcata atccaatgtt gtttgggtcg taatgagcaa ttccgggatg 540

ggaccgacta ctttgggtgt ccgtgtttct ttttattctt atattgtctt atggtcacag 600

catatatagt atatatactg tgatc 625

<210> 414

<211> 625

<212> DNA

<213> Human rhinovirus B99 (strain ATCC VR-1299)

<400> 414

ttaaaacagc gggtgggtat cccaccaccc gacccactgg gtgtagtaca ctggtatttt 60

gtacctttgt atgcctgttt gccatccctc cctctcctct tacccttacc cttattttgc 120

ggtaacttta gaagtttgta aatcacgcgc aatagggtat accacatcca gtggtatgaa 180

gcacaagcaa ttcttgttcc ccggagctgg atataaactg ctaacgtggt tgaaagtcct 240

taaccgttat ccgccagtta actgcgagaa gactagtaac tccatgtttg tttttaagcg 300

ttcgatcagg tggatacccc attcactagt ttggtcgatg aggctgggaa tcccccacag 360

gtaactgtgt ctcagcctgc gtggcggcca acccagcttt tgctgggacg cttactgaca 420

gacatggtgt gaagatccta ttgcgcttaa ctgtgagtcc tccggcccct gaatgcggct 480

aacctcaacc ccggagcctt gtggtgtaaa ccaacactcg caaggtcgta acgagtaatt 540

ctgggacggg accgactact ttgggtgtcc gtgtttcctt tatttcttat tgattgtctc 600

ctatggttac aaatttactg taatc 625

<210> 415

<211> 557

<212> DNA

<213> Human rhinovirus C2 (isolate 470389)

<400> 415

cttgtgctcc ggtacacttg cacgccagtt tgcctcccca tccccgagta acttagaagt 60

ttacaccatg accaacagga ggtggtaaac cataccactt acggtcaagc acttctgttt 120

ccccggcacg agaggaatag actcttttag ggttgaagcc acgagtgtcg ttacccgcgc 180

tagtactacg caaagcctag taataccttg aagctcttct ggttggtcgt tccactagtc 240

acgccctagt agacctggca gatgaggcag gacactcccc actggtgaca gtggtcctgc 300

ctgcgtggct gcctgcacac cctccggggt gtgaagccag aagaaggaca aggtgtgaag 360

agccccgtgt gctactagtg agtcctccgg cccctgaatg cggctaatct taccccacag 420

ctattgcaca caatccagtg tgtatgtagt cgtaatgagc aattgtggga cggaaccgac 480

tactttgggt gtccgtgttt ccttttattc ttatgttttt gcttatggtg acaatacaga 540

catatagtgt tgttacc 557

<210> 416

<211> 483

<212> DNA

<213> Human rhinovirus C6 (strain RvC6/USA/2021/LCP8K8)

<400> 416

ccaataggtg gtgaccatcc aggtcactaa cggtcgagca cttctgtttc cccggtaccc 60

ttgtatacgc ttcacccgag gcgaaaaacg aggttatcgt tatccgcaaa gtgcctacga 120

gaagcctagt agcacttttg aaacctatgg ctggtcgctc agctgcacac ccagcagtag 180

acctggcaga tgaggctaga cactcctcac cagcgatggt ggtctagcct gcgtggctgc 240

ctgcacaccc tatcgggtgt gaagccaaaa gtggacaagg tgtgaagagc ctattgtgct 300

cactttgagt cctccggccc ctgaatgtgg ctaatcctaa ccccgtagct gttgcatgca 360

acccagcgtg tatgcagtcg taatgggcaa ctatgggatg gaaccaacta ctttgggtgt 420

ccgtgtttct tgttttactt ttcattgctt atggtgacaa ttgtaattaa tacacttgtt 480

acc 483

<210> 417

<211> 579

<212> DNA

<213> Human rhinovirus C8 (strain RvC8/USA/2021/7N6PM0)

<400> 417

gtagtactct tgtactccgg tacacttgta cgccagtttt ccctccctac ccccccttgg 60

gtaactttta gaagcatttg cactcagacg aataggcaac caacactcca tgttggtcaa 120

cgtcaagcac ttctgtttcc ccggtattag ggtgttaggc tttccaagag gctgaagcct 180

gaagtatcgt tacccgcaca attactaagc agagcctagt accattttga ccctttcact 240

ggctggtcgt tcaatgagaa aacccactca tagacctggc agatggggct ggaaataccc 300

cactggtaac agtgttccag cccgcgtggc tgcctgtccc ccatcttggg ggaaagccaa 360

tgtaaggaca aggtgtgaag agccgcgttt gctaattgtg agtcctccgg cccctgaatg 420

cggctaatct taccccacag ctattgcatg gaagccacca tgtggatagt cgtaacgggc 480

aactgtggga cggaaccgac tactttgggt gtccgtgttt ccttttattc catttattat 540

ctcatggtga caacgtatac agattatatg ttgttcacc 579

<210> 418

<211> 639

<212> DNA

<213> Human rhinovirus C9 (strain RvC9/USA/2021/96D92H)

<400> 418

ttaaaacagc cgtgtggttg tacccaccac cgggcacatt gtgcgtggta cactggaatt 60

acggttcctt tgtacgcctg ttttccctcc ccgctttctt tctttcttca cttcttgatc 120

gtaacataat tagaagcatg acacacagtc caataggagg cggttaatcc ataaccgtca 180

cggacaagca cttctgtttc cccggatgtg tgaataggct tccaccgagg ctgaagctgt 240

agcattcgtt atccgcacac gtactacgcg aaaactagta acaccttgat tggtggtgtg 300

gtgtttcatt cagcgctacc ccagcgtaga tcagggcaat ggggctacgg aaaccccacc 360

ggcgacggtg tcgtagcccg cgtggtgccc tgcccagatc cttgtgatct gggacaccat 420

accattgaca gggtgtgaag attctagtgt gctacgtgtg agtcctccgg cccctgaatg 480

tggctaacct taaccccgca gctatggtgt gcaaaccagc acattcgtag tcgtaatggg 540

caactgtggg acgggaccaa ctactttggg tgtccgtgtt tccttttatt cttataattt 600

tccttatggt tacaggctta cggttataag tttgtaatc 639

<210> 419

<211> 587

<212> DNA

<213> Human rhinovirus C10 (strain QCE)

<400> 419

catggttacc cacgaggtgc agtggtcttg tattacggta catttccata ccagttttat 60

aaaccctacc ctgaaactta tagaagcttg tacacaccga ccgacaggtg gcaatcatcc 120

agattgtcga aggtcaagca cttctgtttc cccggtatcc ttgtatacgc ttcacccgag 180

gcgaaaaccg aggttatcgt tacccgcaaa gtgcctacga gaagcctagt atcactttgg 240

aaacctatgg ttggtcgctc agctgttttc ccaacagtag acctggcaga tgaggctgga 300

cgctccccac cagcgatggt ggtccagcct gcgtggctgc ctgcacaccc tgtggggtgt 360

gaagccagaa agtggacaag gtgtgaagag cctattgtgc tcactttgag tcctccggcc 420

cctgaatgtg gctaatccta accccgtagc tgttgcatgt aatccaacat gtatgcagtc 480

gtaatgggca actatgggat ggaaccaact actttgggtg tccgtgtttc ttgtttttct 540

ttatatactg cttatggtga caatagtaat tcattactct tgttacc 587

<210> 420

<211> 613

<212> DNA

<213> Human rhinovirus C11 (strain SC9849)

<400> 420

ttgatcccac cagcgggcac aatgtgcgtt gtacactgcg atctcggtcg ctttgtatgc 60

ctgtttttac cacccacccc aactttactg tcttctttat ttcacaagta acatagaagc 120

atgtacatga tgcccagtag gataacgtcc cagacggctg ttttaagggc aagcacttct 180

gtttccccgg atgtgtgaat aggcttcacc cgaggctgaa actgtagcat tcgttatccg 240

catggctact acgcgactgc tagtagcatt ctgtatggca tgtggggttt cgctcagcag 300

agacccagtc tgtagatcag ggtaatgagg ctacacaatc cccactggtg acagtggtgt 360

agcctgcgtg gtgccctgcc cgaggttcat cctcgggacc ctccatgtta gacagggtgt 420

gaagacacta gtgtgctagt catgagtcct ccggcccctg aatgcggcta atcttaaacc 480

tgcagccatc gcatgcatgc cagcgtgtag gtggtcgtaa tgagtaattg cgggatggaa 540

ccgactactt tgggtgtccg tgtttccctt tttacatttg tgtgtctctt atggttacat 600

cattggtgta atc 613

<210> 421

<211> 599

<212> DNA

<213> Human rhinovirus C12 (strain RvC12/USA/2021/044858)

<400> 421

tgtacccacc cacactgtct acggacgttg tacacttgta ttccggtaca cttgtacgcc 60

agtttgccac cctatacacc ccgtaacagt agaagtttac acacacccag accaataggg 120

ggtgttcatc cagttcaccc agggtcaagc acttctgttt ccccggcata ggggtgttag 180

gtttcacccg aggccgaagc tccgattgtc gttatccgca caactactgg acaaagctta 240

gtaacatctt acgatgtagc atggttggtt gttccgccag taccccctgg taaacctggc 300

agatggggct agaagttcct cactggtgac agtgttctag cccgcgtggc tgcctgctcc 360

ctcacgggag aagccatcct attgacaagg tgtgaagagc cccgtacgct agttgtgatt 420

cctccggccc ctgaatgcgg ctaatccaac cctgcagcca ttgcatggaa gccaccatgt 480

ggatggtcgt aatgagtaat tgcgggacgg aaccgactac tttgggtgtc cgtgtttctt 540

gtttttcttt tactgacgtt tatggtgaca atatatatat aacgtatata ttgttcatc 599

<210> 422

<211> 533

<212> DNA

<213> Human rhinovirus C15 (strain RvC15/USA/2021/SUSM75)

<400> 422

ttgccccccc cccttccttc cctgtaacgt agaagcattg taaacggacc aaaaggggac 60

ggcaaaccac gtcgttcaag gtcaagcact tctgtttccc cggcatgcga ggaatagacc 120

ttttcaaggg ttgaagccac aagtgtcgtt acccgcattg gtactacaca gagcctagta 180

ctgctctaga aacttattgg ttggtcgttc agccatttcc cctggtagac ctggcagatg 240

aggcaagaag ttccccactg gtgacagtgt tcttgcctgc gtggctgcct gcacacccta 300

acgggtgtga agccaataag tggacagggt gtgaagagcc gcgtgtgcta ccagtgagtc 360

ctccggcccc tgaatgcggc taatccaacc ccacagctat ggcatgcaag ccagcatgtg 420

cgtagtcgta atgagcaatt gtgggacgga accgactact ttgggtgtcc gtgtttcctt 480

tattctctta tgttttgcct atggtgacaa tattaagtgt taatattgct acc 533

<210> 423

<211> 572

<212> DNA

<213> Human rhinovirus C17 (strain RvC17/USA/2021/T3RVH2)

<400> 423

gtacacttgt attacggtac atttgtacgc cagtttgcct cccttcccta ccttgtaact 60

ttagaagata caactacatg acaaatagga agccaacaag ccatgttggt tattgtcaag 120

cacttctgtt accccggtac tagggtgtta aacttcccca gaggttgaag cccgatgtat 180

cgttatccgc atgattacta agcagagcct agtaacatct tactttatat atgggttggt 240

cgctcagtca gttaccccct gatagacctg gcagatgagg ctggaaacac cccactggta 300

acagtgttcc agcctgcgtg gctgcctgcc cctcttctga ggggaagccc atatgtagac 360

aaggtgagaa gagccgcgtt tgctaatcat gagtcctccg gcccctgaat gcggctaatc 420

caaccccaca gctactgcgt ggaaaccacc acgtgggtag tcgtaatgag caattgtggg 480

acggaaccga ctactttggg tgtccgtgtt tcctgtttta ccttacattg ctcatggtta 540

caacatatat taaatcgtat atgttgttca cc 572

<210> 424

<211> 590

<212> DNA

<213> Human rhinovirus C23 (strain RvC23/USA/2021/ULVLFU)

<400> 424

actcttgcca cccattgggt gtggtgctct tgtattacgg tacacttgca tgccagtttt 60

atatccccct tccccacaat gtaacttaga agctaatcac acgaccaata gggggtggta 120

atccatacca cttaaggtca agcacttctg tttccccggt atgcgtggaa taggcctctc 180

aaggggctga gaccacaatt atcgttaccc gcatgtgaac tatgcggaac ttagtattgc 240

cttgaaagta cattgactgg tcgctcagcc aattcccatg gtagacctgg cagatgaggc 300

aagaaatacc ccactggtga cagtgttctt gcctgcgtgg ctgcctgcac accctccccg 360

ggtgtgaagc caatgtatag acagggtgag aagagtcgag tgtgcttcac atgagtcctc 420

cggcccctga atgcggctaa tccaaccccg cagctgtagt atgcaaacca gcatacatgc 480

agtcgtaatg ggtaactgcg ggacggaacc gactactttg ggtgtccgtg tttcctgttt 540

ttcctttact catgcttatg gtgacaatat tgaatattga tattgtcact 590

<210> 425

<211> 560

<212> DNA

<213> Human rhinovirus C30 (strain USA/2015/CA-RGDS-1045)

<400> 425

cggtacactt gcacgccagt ttgcctccct ttccctttcc cttgccaaac ccccttggaa 60

cttagaagca atacattaga ccaataggag gtaacgatcc atgttaccta aggtcaagca 120

cttctgtctc cccggtatgc gtggaataaa cagtgcaaac ggttgaagcc acaattatcg 180

ttatccgcgc acaaactacg cagagcctag tactaccctg aaagtctgta ggttggtcga 240

tcagctaatc cccatagtag acctggcaga tgaggcaaga cactccccac tggcgacagt 300

ggtcttgcct gcgtggctgc ctgcacaccc tctggggtgt gaagcctact gacggacagg 360

gtgcgaagag ccgagtgtgc tttgtgtgag tcctccggcc cctgaatgcg gctaatccaa 420

ccccacagct gtggcataca agccagtatg tacgcagtcg taacgggtaa ctgtgggacg 480

gaaccgacta ctttgggtgt ccgtgtttcc tgtttttctt tatatatatg cttatggtga 540

caatagtgaa tattgttacc 560

<210> 426

<211> 498

<212> DNA

<213> Human rhinovirus C31 (strain RvC31/USA/2021/B8JUE1)

<400> 426

aactggacca ataggtgata accatccagg ttatcaacgg tcaagcactt ctgtctcccc 60

ggcatagggg tgttaggctt ctcacgaggc taaagccccg acagtcgtta tccgcacaac 120

tactggacaa agcctagtaa caccctacga tgtagtatgg ttggtcgttc caccagtaaa 180

ttccccctgg tagacctggc agatggggct agaaattcct cactggcgac agtgttctag 240

cccgcgtggc tgcctgctcc ctaacgggag aagccatcct actgacaagg tgtgaagagc 300

cccgtacgct agttgtgagt cctccggccc ctgaatgcgg ctaatccaac cctgcagcca 360

ttgcatggaa accaccatgt agatggtcgt aatgagcaat tgcgggacgg aaccgactac 420

tttgggtgtc cgtgtttctt ttgtttcttg tatattgctt atggtgacaa tatatatatc 480

acatatatat tgttcatc 498

<210> 427

<211> 596

<212> DNA

<213> Human rhinovirus C32 (strain USA/CA/RGDS-2016-1008)

<400> 427

atcccaccat gattgttcac tgaacgttgt actcttgtat tacggtacac ttgtacgcca 60

gttttataat cccctcccct tgtaacttag aagcaagtac acacaggcca atagcaagta 120

gcaagccatg ctacttatgg tcaagcactt ctgtttcccc ggtctgacgg gatatgcttc 180

acccgaggca gaaactcaga tgatcgttat ccgcaaagtg cctacgggaa gcctagtagc 240

accctggaaa ctgttaggtt ggtcgctcaa ttgctgaccc cgagcaatag acttggcaga 300

tgaggctaga cataccccac cagcgatggt ggtctagcct gcgtggctgc ctacccaccc 360

taatagggtg ggaagcctat caatggacag ggtgtgaaga gcctactgtg ctcactttga 420

ctcctccggc ccctgaatgt ggctaatcct aaccccgtat ctatcgcatg cgcaccagca 480

tgtaggtagt cgtaatgggc aactacggga tggaaccaac tactttgggt gtccgtgttt 540

cctgttttct ttattatttg cttatggtga caattgtatg aaatacgatt gacacc 596

<210> 428

<211> 596

<212> DNA

<213> Human rhinovirus C34 (strain RvC34/USA/2021/BYRST7)

<400> 428

cgggcatact gtgcgtagta cactgtgatt ccggtcacct tgtacgcctg tttgctaccc 60

caccccaact ttctttcatt cttcacttcc agtaacttag aagttgacac agagtccaat 120

aggatgcagc taatccaagt ctgctacgga caagcacttc tgtttccccg ggtgtgtgaa 180

tagactccaa cagggttgaa gctgtagcac tcgttatccg cacaactact acgcgaatgt 240

tagtaacact ctctaagtat agtgggattt cgctccgcag aaaccccatc tgtagattag 300

ggcaatgagg ctacacagac cccactggcg acagtggtgt agcctgcgtg gtgccctacc 360

tggggtttca taccccagga ttccacttta ctgacagggt gtgaaggcgc tagtgtgcta 420

gttgtgagtc ctccggtccc tgaatgtggc taatcctaac cccgtggcca tttcatgtaa 480

accaacatgc aggtggtcgt aacgagtaat cacgggatgg aaccaactac tttgggtaac 540

cgtgtttcct gttttactta atgtgtgtgt cttatggtta caattataga gtaacc 596

<210> 429

<211> 621

<212> DNA

<213> Human rhinovirus C35 (strain RvC35/USA/2021/70881)

<400> 429

ttaaaacagc tgtgtggttg ttcccaccac caggcacacc gtgcgttgtg cactggaatc 60

tcggttcctt tgcacgcctg ttttcccacc ctcatgtttg tctgtctttg catgtaactt 120

agaagtatca caaacagctc aacaggagtg gctcaaccag agccattacg agcaagcact 180

tctgtctccc cggacgtgtg aataggcctt accaaggctg aagctgtagc gttcgttatc 240

cgctcaccta ctgcgcaaag actagtaact aactagatac ttagtggtgt ttcgctcagc 300

agtaccccac tgtagatcag ggcaatgagg ctacgcatcc cccactggcg acagtggcgt 360

agcctgcgtg gtgccctgcc caggtttcga cctgggacac cgctatgttg acagggtgtg 420

aagaccggcg tgcgctaggt gtgattcctc cggcccctga atgcggctaa tcctaacccc 480

gcagccacca catgcaaccc agcatgtaga tggtcgtaat gagcaattgt gggatggaac 540

cgactacttt gggtgtccgt gtttcctgtt tttctttatt gttgtcctat ggttacaaat 600

ttgtgtatca gatttgtgac c 621

<210> 430

<211> 547

<212> DNA

<213> Human rhinovirus C36 (strain RvC36/USA/2021/PEXCU4)

<400> 430

gcctgttttc cctccccccc ccctccttta atgtaaccta gaagcttgaa caaacgccca 60

ataggatatg accactccat ggtcgtaaag ggcaagcact tctgtctccc cgggtgtgtg 120

agtaggctct aacagggctg aagctgtagc actcgttatc cgcacatcta ctacgcagag 180

gctagtagga ccatgtcaat cctttaggcg tttcgctcag cgtgtacccc cacgtagatc 240

agggcaatgg ggctacacat tccccatcgg cgacgatggt gtagcccgcg tggtgccccg 300

cctaggccta cacggcctag gacgccaaag gaaagacagg gtgtgaagac ccgagtgtgc 360

taggtgtgag tcctccggcc cctgaatgtg gctaatccta accccgtagc catggtgtac 420

aagccagtac aaacatggtc gtaatgggca actatgggat ggaaccaact actttgggtg 480

tccgtgtttc tctttttacc tttcactgca tttatggtta caagcttgta aatacagact 540

tgtaatc 547

<210> 431

<211> 547

<212> DNA

<213> Human rhinovirus C39 (strain RvC39/USA/2021/71206)

<400> 431

gcctgttttc cctccccccc ccctccttta atgtaaccta gaagcttgaa caaacgccca 60

ataggatatg accactccat ggtcgtaaag ggcaagcact tctgtctccc cgggtgtgtg 120

agtaggctct aacagggctg aagctgtagc actcgttatc cgcacatcta ctacgcagag 180

gctagtagga ccatgtcaat cctttaggcg tttcgctcag cgtgtacccc cacgtagatc 240

agggcaatgg ggctacacat tccccatcgg cgacgatggt gtagcccgcg tggtgccccg 300

cctaggccta cacggcctag gacgccaaag gaaagacagg gtgtgaagac ccgagtgtgc 360

taggtgtgag tcctccggcc cctgaatgtg gctaatccta accccgtagc catggtgtac 420

aagccagtac aaacatggtc gtaatgggca actatgggat ggaaccaact actttgggtg 480

tccgtgtttc tctttttacc tttcactgca tttatggtta caagcttgta aatacagact 540

tgtaatc 547

<210> 432

<211> 557

<212> DNA

<213> Human rhinovirus C40 (strain RvC40/USA/2021/70389)

<400> 432

cttgtgctcc ggtacacttg cacgccagtt tgcctcccca tccccgagta acttagaagt 60

ttacaccatg accaacagga ggtggtaaac cataccactt acggtcaagc acttctgttt 120

ccccggcacg agaggaatag actcttttag ggttgaagcc acgagtgtcg ttacccgcgc 180

tagtactacg caaagcctag taataccttg aagctcttct ggttggtcgt tccactagtc 240

acgccctagt agacctggca gatgaggcag gacactcccc actggtgaca gtggtcctgc 300

ctgcgtggct gcctgcacac cctccggggt gtgaagccag aagaaggaca aggtgtgaag 360

agccccgtgt gctactagtg agtcctccgg cccctgaatg cggctaatct taccccacag 420

ctattgcaca caatccagtg tgtatgtagt cgtaatgagc aattgtggga cggaaccgac 480

tactttgggt gtccgtgttt ccttttattc ttatgttttt gcttatggtg acaatacaga 540

catatagtgt tgttacc 557

<210> 433

<211> 609

<212> DNA

<213> Human rhinovirus C41 (strain USA/CA/2016-RGDS-1006)

<400> 433

gttgttccca cccctaccac ccatgtggtg tagtgctctt gtattacggt acacttgcac 60

gccagtttgc ctccccaccc cctttccatt tacacccaac cttatgtaac ttagaagcag 120

tacactggac caataggtgg taacaatcca tgttaccaaa ggtcaagcac ttctgtctcc 180

ccggcatgcg tggaataaac agtgcaaacg gttgagacca caaatgtcgt tacccgcgca 240

tgaactacgc agagcctagt atctccctga aagtccattg gttggtcgtt cagctaaacc 300

ccatagtaga cctggcagat gaggcaagac gcaccccact ggcgacagtg gtcttgcctg 360

cgtggctgcc tgcacaccct tcggggtgtg aagccaattg acagacaggg tgtgaagagc 420

cgagtgtgct tcatgtgaat cctccggccc ctgaatgcgg ctaatctaac cccgcagctg 480

tggcacacaa gccagtgtgt atgcagtcgt aacgggcaac tgcgggacgg aaccgactac 540

tttgggtgtc cgtgtttcct tttattcttt tatcaagtac ttatggtgac aataataaat 600

attgttacc 609

<210> 434

<211> 547

<212> DNA

<213> Human rhinovirus C42 (strain RvC42/USA/2021/278730)

<400> 434

gcctgttttc cctccccccc ccctccttta atgtaaccta gaagcttgaa caaacgccca 60

ataggatatg accactccat ggtcgtaaag ggcaagcact tctgtctccc cgggtgtgtg 120

agtaggctct aacagggctg aagctgtagc actcgttatc cgcacatcta ctacgcagag 180

gctagtagga ccatgtcaat cctttaggcg tttcgctcag cgtgtacccc cacgtagatc 240

agggcaatgg ggctacacat tccccatcgg cgacgatggt gtagcccgcg tggtgccccg 300

cctaggccta cacggcctag gacgccaaag gaaagacagg gtgtgaagac ccgagtgtgc 360

taggtgtgag tcctccggcc cctgaatgtg gctaatccta accccgtagc catggtgtac 420

aagccagtac aaacatggtc gtaatgggca actatgggat ggaaccaact actttgggtg 480

tccgtgtttc tctttttacc tttcactgca tttatggtta caagcttgta aatacagact 540

tgtaatc 547

<210> 435

<211> 610

<212> DNA

<213> Human rhinovirus C43 (strain SC174)

<400> 435

ttaaaactga accatagttg ttcccactgt ggttgcccaa ggggcgtggt ggtcttgtat 60

tacggtacat ttccatgcca gttttatcac cccttcccga aacatagaag aaatgcacac 120

accgaccaat aggaggtggt caaccagacc actaaaggtc aagcacttct gtttccccgg 180

ttggcttgga tatgcttcac ccgaggcaaa aaccgagcca atcgttatcc gcaaagtgcc 240

tacgagaagc ctagtagcac ccttgaagcc aatggttggt cgctccgttg caaccccagc 300

agcagacctg gcagatgagg ctgagcattc cccaccagcg atggtggctc agcctgcgtg 360

gctgcctgcg catccttcgg gatgcgaagc cagaaagtgg acaaggtgtg aagagcctat 420

tgtgctcact ttgagtcctc cggcccctga atgtggctaa tcctaacccc gcagctacta 480

cacataagcc aatgtgcagg tagtcgtaac gggcaactgt gggatggaac caactacttt 540

gggtgtccgt gtttccttgt tatctttttg aatgcctatg gtgacaattg tacagtatac 600

atttgttacc 610

<210> 436

<211> 608

<212> DNA

<213> Human rhinovirus C47 (isolate CA-RGDS-1001)

<400> 436

ggcatgggtt gtacccaccc atgccaccca agtggtgtag tgctcctgta tcccggtaca 60

cttgcacgcc agtttgccct cccttttccc caagtaactt agaagcttac acacaggcca 120

ctaggaggtg gtaatccata ccactgatgg tcaagcactt ctgtctcccc ggcacgcatg 180

gaatagacct tgaaaagggt tgaagccata aatgtcgtta tccgcattgg tactacacaa 240

agcctagtaa tgccttgaaa cttctacggt tggtcgttcc gctagatgca cccctctagt 300

agacctggca gatgaggcag ggcaaacccc actggcgaca gtggccctgc ctgcgtggct 360

gcctgcgcgc ccacttgggt gcgaagccga tgaacagaca aggtgtgaag agccccgtgt 420

gctaccaatg agtcctccgg cccctgaatg cggctaatcc aaccccacag ctattgcaca 480

caaaccagtg tgtatgtagt cgtaatgagt aattgtggga cggaaccgac tactttgggt 540

gtccgtgttt ccttttattc tttaaatttg cacatggtga caatattggt agtatcagta 600

ttgtcatc 608

<210> 437

<211> 438

<212> DNA

<213> Human rhinovirus C50 (strain human/Australia/SG1/2008)

<400> 437

ttccccggac gtgtgaatag gctccaccag gggctgaagc tgtagcgttc gttacccgca 60

ctgctactac gcaagggtta gtatctaact gaaaacattg tggagtttcg ctcagcagaa 120

ccccactgta gatcggggta atggggctac acaaccccca ccggcgacgg tggtgtagcc 180

cgcgtggtgc cccgcctgag gcttgtgcct caggacgcca caatgttgac agggtgagaa 240

ggcctacgtg tgctagcagt gagtcctccg gcctctgaat gcggctaatc ctaaccccat 300

agccatcaca cacaatccag tgtgcaggtg gtcgtaacgg gtaactatgg gatggaaccg 360

actactttga gtgtccgtgt ttcctatttt acctttacat gtcttatggt cacaatatag 420

tgtatacata ttgtgatc 438

<210> 438

<211> 641

<212> DNA

<213> Human rhinovirus C51 (isolate LZ508)

<400> 438

ttaaaacagc cgtgtggttg tacccaccac cggacacact gtgtgttgtg cactggaatc 60

tcggttcctt tgcatgcctg ttttaaaccc cccttccccc actctttcta tcattctttt 120

cttcctaatg taacttagac gttataccac agtccaatag gacgtggcca atccaggcca 180

tgacggacaa gcacttctgt ctccccgggc gtgtgaatag gcctcaacaa ggctgaagct 240

gtagcgctcg ttatccgcat ttgtactacg cgatggctag tatcatcctt ctcttggtct 300

tggcgtttcg ctcagcgtgg taacccccca cgtagatcag ggcaatgagg ctgcatatgc 360

cccaccggcg acggtgatgc agcctgcgtg gtgccccgcc tgggttaacc cccaggacgc 420

cacaatcaag acagggtgag aagacctgag tgtgctacag atgagtcctc cggcccctga 480

atgcggctaa tcctaaccct gcagctatcg tatgcaaacc agcatattgg tagtcgtaac 540

gagcaattgc gggacggaac cgactacttt gggtgtccgt gtttctcttt ttatttgaaa 600

tgttgcttat ggttacaagt tttggtatta aacttgtgac c 641

<210> 439

<211> 607

<212> DNA

<213> Human rhinovirus C54 (isolate D3490)

<400> 439

ttaaaacagc tgtgtggttg ttcccaccac caggcacact gtgcgttgta cactgggatt 60

ccggtcactt tgtacgcctg tttgctatcc cccccaactt atgtaattta gaagatgtac 120

acaacgccca ttaggatgcg gccaaaccag gtccgcttag ggcaagcact tctgtttccc 180

cgggtgtgtg aatagactct aacagggttg aagctgtagc actcgttatc cgcgcaacta 240

ctacgcgaat gttagtagca tcctgtgttg catttgggat ttcgctccgc agaaaacccc 300

atctgtagat tagggcaatg aggctacaca taccccactg gcgacagtgg tgtagcctgc 360

gtggtgccct acccaggcct tcttggcctg ggattccact tacaagacag ggtgtgaagg 420

cactagtgtg ctagttgtga gtcctccggc ccctgaatgc ggctaatctt aaccccgtag 480

cccccgcaag taaaccaact tgtaggtggt cgtaatgagt aattacggga tggaaccgac 540

tactttgggt gtccgtgttt ccttttattc tttatatttg catcctatgg ttacaacata 600

agtaatc 607

<210> 440

<211> 603

<212> DNA

<213> Human rhinovirus C56 (strain RvC56/USA/2021/466615)

<400> 440

cacccacacc gtctacggac gtagtactct tgtatctcgg tacatttgta cgccagtttt 60

ccctccctcc ccccccttca tgtaacttat agaagcttga acatacaggc gaataggaag 120

ccaacaaacc atgttggtaa acgccaagca cttctgtttc cccggtatta gagtgttagg 180

cttctcacga ggctgaagct tgatatatcg ttacccacac agttactaag cagagcctag 240

taacactttg atctctatac tggctggtcg ctccgtcaga aaccccctga cagacctggc 300

agatggggct ggaaattccc cactggtgac agtgttccag cccgcgtggc tgcctgtccc 360

ccttccttgg gggaaagcca atatatagac aaggtgccaa gagccgcgtt tgctaattgt 420

gagtcctccg gcccctgaat gcggctaatc caaccccaca gctatcgcat ggattccacc 480

atgtaggtag tcgtaacggg caactgtggg acggaaccga ctactttggg tgtccgtgtt 540

tccttttata ccttatttta tctcatggtg acaacatata ttgataagta tatgttgacc 600

acc 603

<210> 441

<211> 810

<212> DNA

<213> Enterovirus E (isolate HeN-A2)

<400> 441

tttaaaacag cctgggggtt gtacccaccc ccggggccca cgtggcgcca gtactctggt 60

tcgattagaa cctttgtacg cctgtttttc cccaccctta aataaattaa gatctctacc 120

aatgtgggga gtagcccgac tccgcaccga tacgtcgcac cagcagaccg gttcgcctag 180

gacccttctg cggattggta tgagttccca cccctgcaac ttagaagtct tcacaaaccg 240

accaatagcg gcgttgcacc cagcagcgca acggtcaagc acttctgttt ccccggcgcg 300

ttgcgtcgtt acccgcccaa tctactacgg gaagcccagt atggtcccaa ttgattgcgc 360

agttgcgttc ggccacaacc ccagtggtag ctctgaggaa tggggctcgc ctttccccca 420

cagcaatgtg gtggcttgcc cgcgtgtgtc ctcgggttca ctcaatgagt gttcactgca 480

atctcaagca agacctgaag agcctactgt gctagactgg ttttcctccg gagccgtgaa 540

tgctgctaat cccaacctcc gagcgtgtgc gcacaaacca gtgttgctac gtcgtaacgc 600

gtaagttgga ggcggaacag actactttcg gtactccgtg tttccttgtt ttatttaaat 660

tttttatggt gacactgact gatatttgtg agtttgctcg cttgcctttg aatattgccc 720

tatattactt gattgcattc cataaaacaa ctcaactttc agtcgaactt attgactttg 780

cagtgttttt gaatcgtgct cacaagaaat 810

<210> 442

<211> 821

<212> DNA

<213> Enterovirus F (isolate HeN-B62)

<400> 442

tttaaaacag cctgggggtt gttcccaccc ccggggccca cgtggcgcca gtactctggt 60

acgctagtac ctttgtacgc ctgtttttcc ctcccttaaa taaattaaga ttaccactac 120

tgaggggagt agtccgactc cgctccagca ctgctgcacc agtgagctgg tacgctagta 180

ccttcgcacg gagtagatgg tatccccacc ccgcaactta gaagcaaagt acacaaccga 240

ccaatagcgg ctcagcatcc agctgtgcaa cggtcaagca cttctgtttc cccggtccgc 300

aaggatcgtt acccgcccat gctactacga gaagcctact aaccactgaa tccatctaga 360

ggttgcgctc agccacaaac ccagtggtag ctctgagtga tggggctcgc atttcccccg 420

tggtaacacg gttgcttgcc cgcgtgtgca ctcgggttcg acctatggtc gttcacctcg 480

aaatggtgta ggtggccaag agcctattgt gctagcgtgg ttttcctccg gagccgtgaa 540

tgctgctaat cccaacctcc gagcgtgtgc gcacaatcca gtgttgctac gtcgtaacgc 600

gtaagttgga ggcggaacag actactttcg gtactccgtg tttcctttat tttatattta 660

tattttatgg tgacaattgc tgagatttgt gatttagcga ctttaccatt gaacattgca 720

ttgtactacc ttgtctcgtt tcacaaagtc tcagaaatac ccagtgcata cattgatcta 780

cttgtcctct tgaatttaaa acataaatta gagagtacaa a 821

<210> 443

<211> 797

<212> DNA

<213> Enterovirus G (EV-G/Pig/JPN/Kana-Uchi13/2019/G1_PL-CP)

<400> 443

gttgttccca cccacagggc ccactgggcg ccagtacact ggtatgccag taccttggta 60

tcacggtacc attgtacgcc agtacctttg tacgcctgtt ttatactccc tcccccacat 120

gtaaccttag aagtttaaat aaacaaggac caataggagt ccaacaccca gttggattgc 180

ggtcaagcac ttctgtctcc ccggacctag tagtgatagg ctgtacccac ggccgaagat 240

gaacccgtcc gttatccggc cagctacttc gagaagccta gtaacatcaa agatctgtct 300

tggcgtttcg ctcagcgcgt tccccccgcg tagatcgggc tgatgggtct ccgcataccc 360

cacgggcgac cgtggcggag gccgcgtggc ggcccgccta tggcgaaagc cataggacgc 420

catttcagtg acagggtgtg aagagcctat tgagctagtt ggtagtcctc cggcccctga 480

atgcggctaa tcctaaccac ggagcgtcca ccagcaaacc agctgggagg gcgtcgtaac 540

gggtaactct gtggcggaac cgactacttt gggtgtccgt gtttcctttt gatcctattt 600

tggctgctta tggtgacaac gataagttgt tatcataaag cttttgggtt ggccacctgg 660

aaaaagttat cagtgtttga cattgttcgg ctctcacgcc taccaataaa acaagcttta 720

tatttgttaa tttcatttta tccatccaag gatattactg aatttcactt acaactcttg 780

cttacactat caacaag 797

<210> 444

<211> 847

<212> DNA

<213> Enterovirus I Dromedary camel enterovirus (strain 19CC)

<400> 444

ttaaaacagc ctgggggttg ttcccacccc cagggcccac gtggcgcaag tactctgctt 60

cgttagaagc cttgtacgcc tgttttcccc tccttaaaca aattaagatt gccacttccg 120

aggggagtag tccgactccg ctccgatacg tcgctccagt gcgctagtac gcttagtact 180

ttctcacggg gatagtggta tccctcccta gtaatttaga agacttgaaa aaccgaccaa 240

taggcacctc gcatccagcg gggtaaaggt caagcacttc tgtttccccg ggtcgagtag 300

cgatagactg tgcccacggt cgaaggtgaa acaacccgtt atccgacttt gtacttcggg 360

aagcctagta ccaccaaaga ttatgcttgg ggtttcgctc agcacgaccc tggtgtagat 420

caggccgatg gatcaccgca ttcctcacag gcgactgtgg cggtggtcgc gtggcagcct 480

gccgatgggg caacccatcg gacgccaagc atatgacagg gtgtgaagag cctactgagc 540

tacaaagtat tcctccggcc cctgaatgcg gctaatccca accacggagc atttgctacc 600

aaaccaggta gtggaatgtc gtaacgggta actctgtggc ggaaccgact actttgggtg 660

tccgtgtttc cttttaattt atcattctgt atatggtgac aactatagtg ctatctcgat 720

ttgcattact attgttgaga ttaaaacttt attacattgt tgcattttac cctttgagtg 780

agttttcacc tgaacagatt aatttactca tcctgtttat atattacaag cagaaatact 840

tgcaaag 847

<210> 445

<211> 747

<212> DNA

<213> Bovine enterovirus GX20-1

<400> 445

tgtacgcctg ttttcccctc cttaaataaa ttaagattac cactactgag gggagtagtc 60

cgactccgct ccagcactgc tgcaccagtg aattggtatg attagtacct cttcacggag 120

tagatggtat tcctaccccg caacttagaa gtttatcaac aaaccgacca ataggtgcac 180

agtatccagc tgtgtaacgg tcaagcactt ctgtttcccc ggtccgccag gatcgttacc 240

cgcccgaccc actacgagaa gcctagtaac tagccaagtg accgcgaagt tgcgctcagc 300

cacaacccct gtggtagctc tggaagatgg ggctcgcact tcccccgtgg taacacggtt 360

gcctgcccgc gtgtgcttct gggttcggcc ttgtgccgtt cacttctacg tcatgtaact 420

agccaagagc ctattgtgct gggacggttt tcctccgggg ccgtgaatgc tgctaatcct 480

aacctccgag cgtgtgcgca caatccagtg ttgctacgtc gtaacgcgta agttggaggc 540

ggaacagact actttcggta ccccgtgttt ccttttatta ctatattatt taatggtgac 600

aattgcagaa atttgtgatt tagcgacttt gccattggac atagcattat attgtttaat 660

tgcttttcac aaaacgtctg atatacccaa ctcttatatt gatctacttg tctttctttg 720

cttgaagaat aaattagaga aagcacg 747

<210> 446

<211> 819

<212> DNA

<213> Goat enterovirus (isolate NMG-F37)

<400> 446

ttttaaaaca gcctgggggt tgttcccacc cccggggccc acgtggcgct agtgctctgg 60

ttcgtctaga acccttgcac gcctgttttc ccccccaaac aaattaagct taccggtgct 120

gaggggagta gtccgactcc gctccactac ggtgcaccag tagactggta ccgcggtacc 180

tttctacgga gttgccggta tccctcaccc gtaacttaga agtttgaaca cgaaccgacc 240

aacagcggct cggcagccag tcgggcaacg gtcaagcact tctgtctccc cggtccgcaa 300

ggatcgttac ccgccccgac tactacggga agcttagtac ctggccaagc ttggaagagg 360

ttgcgttcgg ccacaacccc agtggtagct ctgagtgatg gggctcgcga catccccctt 420

ggcgacaagg acgcttgccc gcgcgtgcac tcgggtccac cttatggtgt tcacctcgac 480

taagcgtaac tagagaagcg cctactgtgc tagttgggtt ttcctccggg gccgtgaatg 540

ctgctaatcc caacctccga gcgtgtgcgc acaatccagt gttgctacgt cgtaacgcgt 600

aagttggagg cggaacagac tactttcggt accccgtgtt tcctttattt tattcattca 660

atttatggtg acaattgttg aaatttgtga tttagcgaaa ttaccactta atttggcact 720

atattacttg gttgctttcc acaagacctc tgacataccc agttcttaca ttgatctgct 780

agtttatctg aacttgcaac acaagttgaa tcaagtaaa 819

<210> 447

<211> 1339

<212> DNA

<213> Aimelvirus 1 (strain gpai001)

<400> 447

gtagaaaccc cctggccccc tcttcaacgc agcgggtggt ctgcgtaatc taccacccag 60

tcgggaagag accccgactc taacttcaag tctcaagcaa cccaaccatc attgtaaccg 120

ggactgaact tccttcggtc ttggtcgtgg gactaacaga gtttctgttt gagtttccgg 180

cccaggactt aaggttggaa tgttccaaga taagtttaag tacctcctgg atctgcaacc 240

gcgggactaa caggtgaacc ctgtgagttt ccggcccagg atttaggatg acatgttcca 300

agtcagatgc caacgacttg gtaaggacga atttgcccgc actacgttac ggttcccgcc 360

cgggacaact ggtaccccat taggctacaa catggctgaa aagggtattg ggtccccccg 420

gattgtgtcc gttcgtagtg tgtgtaacgt ggtttaccat ctccactaac attggactaa 480

gcatttcatc tttcctcccc gattgtgtac tcacttggct aacgctgggt ggtcgcggtt 540

gggtccttga tttacttttt ctcgtctaag cattccgact gtcctccccg attatgtgct 600

cattcagtta actgctgggt ggtcatgact aacatcgagg aaccttctgt ccacgcttac 660

tttgagctcc ggtcgcttga cgcttgtagg gcgatagggt tatcttcctg acaacatctt 720

tattctacct ccataggctc tatctatgga gacggagtgt ggcacccgtc ccttctttgg 780

gagcttcggt agtgacgccc tttgtcactc tcgccagccg aggcatgcct ggtgccaggt 840

agcaaagaaa gcatatgttt aaggacttga ctgatttagc gcaagagttt gtagcgatgt 900

ccatagtgtc tgcggattcc ccacacggcg acgtgtgccg cggaggccaa aagccacggt 960

gttcacagca cccctatgga tgcccacaga ccccagtggg cactcttgtt gccggacttt 1020

caggaaatta ggcataggct cttctcaaac tcctggcatt ggactaggta agaatgcccc 1080

ggaggtaccc cagtactcct tcgggagtct gggatctgac cgggggcccc acaaacatgc 1140

tttacgtgtt tcgtgcggtc aaaaattgtc taactagtcc caaccttgaa caagggattg 1200

ttctttcctt tttattactg agactggcct atggtgacaa cagagattga ctgtgaatac 1260

agttattttc tggtgttcat catttggttt ttctccgtgc tcttttacct ttgtggtatt 1320

tgttctttag ataggcaaa 1339

<210> 448

<211> 970

<212> DNA

<213> Ampivirus A1 (strain NEWT/2013/HUN)

<400> 448

ttaaaatgaa aatgtgcgag catttagctc gcacttttca ttttaaatat gtccacagcc 60

ctcaagggac ttagtccctt gtccgcctcg gcgacttaat atttaacttg tagttactta 120

tataaagccc gtaatttata cgatacatac aaaactccag catccactct gactgccata 180

gtggaataaa ccaagaccac tctctacatc cggagagtca aatccattgg acgaccattt 240

gatactattc tacaaaatct attttgatca tattatatta agcattaagc gtcattgact 300

tggttgatta attagtttca ctgattaatc gagtcaacaa acaacttcct aaattaatat 360

aattttgctc aaacgagaat ttcagaatat tatgcgacca tttaactatg tttacttaag 420

catcattgac ttttgagtca acaaatacct taagtttccg ctcatctcct taccttcata 480

cgcaggccca taagcctata ttatttacct ctatttataa ttttaatcat taatctgcac 540

aattccaccg tttagacatt attagggttt ataacaacac taataattat tgtatgaaaa 600

ccagtagttc cagcgcgtcg tggcctattg acccccatac aaagttcata agaatagttt 660

tgctgttttc agtcattcat tctttccctt aaaaggtttg gtcacctata agtccagctc 720

attacatcga gtatggcaag tctgcccaac atttgttata tctgactctg gtgataataa 780

atgtggcggg ataaagttac aagtctagtt atatagacag ctttagctca aaaccccaat 840

ttcccccctt gtgtattatt attgtattat taattgtaat tatgtattta atgtaaattg 900

tacttttatc tatccaactt cttatttcaa ttttctatca agactttttt cttgtcgaaa 960

ctcaatatct 970

<210> 449

<211> 918

<212> DNA

<213> Equine rhinitis A virus (strain PERV-1)

<400> 449

ttgaaagggg gttgaatgtt tgactgtgca gcagggaggc catgggtacc ccacttttag 60

gagatgacca aacgcagtaa ccgcaagcaa ttgcctgtag cgtcagtaaa acgcaataca 120

caagatttga gcctgtagcg tcagtaaaac gctgcaacca caagctattg actgtagcgt 180

cagtaaaacg caaacattct tgtggcgctc gcgtagcgct caagtgcaga gcttcccggc 240

tttaagggtt actgctcgta atgagagcac atgacatttt gccaagattt cctggcaatt 300

gtcacgggag agaggagccc gttctcgggc acttttctct caaacaatgt tggcgcgcct 360

cggcgcgccc cccctttttc agccccctgt cattgactgg tcgaaggcgc tcgcaataag 420

actggtcgtt gcttggcttt tctattgttt caggctttag cgcgcccttg cgcggcgggc 480

cgtcaagccc gtgtgctgta cagcaccagg taaccggaca gcggcttgct ggattttccc 540

ggtgccattg ctctggatgg tgtcaccaag ctggcagatg cggagtgaac cttacgaagc 600

gacacacctg tggtagcgct gcccagaagg gagcggagct cccccgccgc gaggcggtcc 660

tctctggcca aaagcccagc gttaatagcg ccttctggga tgcaggaacc ccacctgcca 720

ggtgtgaagt ggactaagtg gatctccaat ttggcctgtt ctgaactaca ccatctactg 780

ctgtgaagaa tgtcctgaag gcaagctggt tacagccctg atcaggagcc ccgctcgtga 840

ctctcgatcg acgcggggtc aaaaactgtc taagcagcag cagaaacgcg ggagcgtttc 900

tttttcctca tttgtttc 918

<210> 450

<211> 1045

<212> DNA

<213> Foot-and-mouth disease virus - type A (isolate A/BR19-16_08dpi_CB-RF)

<400> 450

tgaaaggggg cgctagggtc tcacccctag cgtgccaacg acagtccccg cgttgcactc 60

cacactcacg ttgtgcgtgc gcggagctcg atggactatc gttcacccac ctacagctgg 120

actcacggca ccgtgtggcc acttggctgg attgtgcgga cgaacaccgc ttgcgcttct 180

cgcgtgaccg gttagtactc tcaccacctt ccgcccactt ggttgttagc gctgtcttgg 240

gcactcctgt tgggggccgt tcgacgctcc gcgagtttcc ccgcacggca actacggtga 300

tggggccgta ccgcgcgggc tgatcgcctg gtgtgcttcg gctgtcaccc gaagcctacc 360

tttcaccccc cccccccaag ttttacgtcg ttcccgacgt aaagggatgt aaccacaagc 420

ttactaccgc ctttcccggc gttaaaggga tgtaaccaca agacttacct tcacccggaa 480

gtaaaacggc aacttcacac agttttgccc gttttcatga gaaatgggac gtctgcgcac 540

gaaacgcgcc gtcgcttgag gaggacttgt acaaacacga tctaagcagg tttccccaac 600

tgacacaaac cgtgcaattt gaaactccgc ctgggctttc caggtctaga ggggtgacac 660

tttgtactgt gtttgactcc acgttcgatc cactggcgag tgttagtaac aacactgctg 720

cttcgtagcg gagcatgacg gccgtgggac cccccccttg gtaacaagga cccacggggc 780

caaaagccac gtccgaatgg acccgtcatg tgtgcaaacc cagcacagta gctttgttgt 840

gaaactcact ttaaagtgac attgatactg gtactcaagc actggtgaca ggctaaggat 900

gcccttcagg taccccgagg taacacgtga cactcgggat ctgagaaggg gaccggggct 960

tctataaaag cgcccggttt aaaaagcttc tatgtctgaa taggtgaccg gaggccggca 1020

cctttctttt aattacactg gactt 1045

<210> 451

<211> 1094

<212> DNA

<213> Foot-and-mouth disease virus - type Asia 1 (isolate Mazbi/QOL-UVAS-Pak/2006)

<400> 451

ttgaaagggg gcgctagggt ctcaccccta gcacgccaac gactgctcct acgctgtgct 60

acacacttac gtgtgtacat gcgggaaccg atggactatc gttcacccac ctacagctgg 120

actcacggca ccgcgtggcc atgctagctg gattgtgcgg acgaacgccg cttgcgcttc 180

ggcgtgaccg gttaatactc ttaccactct ccgcctactt ggtcgttagc gctgtcttgg 240

gcactcctgt tgggggccgt tcgacgctcc acggtttccc ccgtgtgaca cactacggtg 300

ttgtggtcac accgtgcgag taggcccacc cccctccccc tttccccccc caatcctccc 360

cgggccccct cccccccccc ccaaagattt accgtcgctc ccgacgttaa agggagataa 420

ccacacgctt gagaccgtct tgtccgacgt taacggactg gaaccacaag cttataccgc 480

ctttcccggc gttaacggga tgtaaccaca agatggacct tcacccggaa gtaaaacggc 540

acacacacac agttttgccc gttttcacga gaaacgggac gtctgcgcac gaaacgcgcc 600

gtcgcttgag gaggacttgt acaaacacga tctacgcagg tttcccaacc gacacaaccc 660

gtgaaacttg aaactccgcc tggtctttcc aggtctagag gggtgacatt ttgtactgta 720

attgactcca cgctcggtcc actagcgggt gttagtagct gtattgttgc ttcgtagcgg 780

agcataatgg ccgtgggaac tcccccttgg taacaaggac ccacggggcc gaaagccacg 840

tcctaacgga cccattatgt gtgcaacccc agcacggcaa cttactgtga aaaccacttt 900

aaggtgacac tgatactggt actcaactac tggtgacagg ctaaggatgc ccttcaggta 960

ccccgaggta acacgcgaca ctcgggatct gagaagggga ccgggacttc tttaaaagtg 1020

cccggtttaa aaagcttcta tgcctgaata ggcgaccgga ggccggcgcc tttcttttga 1080

ttactactgc tatt 1094

<210> 452

<211> 1091

<212> DNA

<213> Foot-and-mouth disease virus - type C (isolate KEN/1/2004)

<400> 452

ttgaaagggg gcgctagggt ctcatcccta gtacgccaac gacagctcct gcgttgcact 60

ccacacttac gtgtgtgtgc gcgcgggaac cgttggactt ccgtccaccc acctacagct 120

ggactcacgg caccgcgtgg ccatttcagc tggattgtgc ggacgaacac cgcttgcgca 180

tctcgcgtga ccggttagta ctcttaccac cttccgccta cttggtcgtt agcgctgtct 240

tgggcactcc tgttcggggc tgttcggcgc tccacggtct cccccgcgca gcagactacg 300

gtgttggggc cgcctcgtgc gagccgctcg cctggcgtgc ttcggctgtc actcgaagcc 360

tgcctttcac cccccccccc ccaagtatta acgtcgggaa cgacgttaaa gggaggtaac 420

cacaagcttt gtaccgtctt tcgacgttaa agggaagtaa ccacaagctt gacaccgcat 480

accccggcgt taaaggggat gaaaccacaa gatacacctt cacccggaag taaaacggca 540

cattcgctta cttttgcccg ttttgagaaa tgggacgtca gcgcacgaaa cgcgccgtcg 600

cttgaggaag acttgtacaa acacgatcta cgcaggtccc cccaactgac acaaaccgtg 660

caacttgaaa ctccgcctgg tctttccagg tctagaggag tgacactttg tactgtgctt 720

gactccacgc tcggtccact agcgagtgtt agtaacagta ctgttgtttc gtagcggagc 780

atggtggccg tgggaacccc tccttggtga caaggaccca cggggccgaa agccacgtcc 840

tgacggacct accatgtgtg caaccccagc acaacaacct tactgtgaaa accactttaa 900

agtgacactg atactggtac tcaaccactg gtgataggct aaggatgccc ttcaggtacc 960

ccgaggcaac acgcgacact cgggatctga gaaggggact gggacttctt taaaagtgcc 1020

cggtttaaaa agcttctatg cctgaatagg cgaccggagg ccggcgcctt ttcccttatt 1080

aacactactt t 1091

<210> 453

<211> 1094

<212> DNA

<213> Foot-and-mouth disease virus O (isolate o6pirbright iso58)

<400> 453

ttgaaagggg gcgctagggt ttcaccccta gcacgccaac gacagtccct gcgttgcact 60

ccacacttac gttcgcgtgc acgcgggacc cgatggactt tcgttcaccc acctacggtt 120

ggactcacgg caccgcgtgg ccattttagc tggaccgtgt ggacgaacac cgcttgcgct 180

tctcgcgtga ccggctagta ctctgaccac tctccgccta cttggtcgct gcgctgtctt 240

gggcactcct attggggtcg ttcgacgctc cacgggctcc cccgtgcggc gggctacggt 300

gatggggccg tttcgcgcgg gctgatcgcc tggtctgctt cggctgtcac ccgaagcccg 360

cctttcaccc ccccccccct aagttttacc gtcgtttccg acgtaaaggg gaagtaacca 420

caagcttgac accgcctgtc ccggcgttaa agggaagtaa ccacaagctt acaaccgcct 480

accccggtgt taatgggatg taaccacaag atacaccttc acccggaagt aaaacggcaa 540

attcacacag ttttgcccgt ttttcatgag aaacgggacg tctgcgcacg aaacgcgccg 600

tcgcttgagg aggacttgta caaacacgat ctaaacaggt ttccccaact gacatacacc 660

gtgcaatttg aaactccgcc tggtctttcc aggtctagag gggtaacact ttgtactgtg 720

cttgactcca cgctcggtcc actggcgagt gttagtaaca gcactggtgc ttcgtagcgg 780

agcatggtgg ccgtgggaac tcctccttgg taacaaggac ccacggggcc gaaagccacg 840

tcctgacgga cccaccatgt gtgcaacccc agcacggcaa cttttctgtg aaactcactc 900

taaggtgaca ctgatactgg tattcaagta ctggtgacag gctaaggatg cccttcaggt 960

accccgaggt aacacgcgac actcgggatc tgagaagggg actggggctt ctgtaaaagc 1020

gcccagttta aaaagcttct atgcctggat aggtgaccgg aggccggcgc ctttccatta 1080

taactactga cttt 1094

<210> 454

<211> 1010

<212> DNA

<213> Foot-and-mouth disease virus - type SAT 1 (isolate TAN/3/80)

<400> 454

ttgaaagggg gcactagggc gagaccctag tgtcgcgtaa cgaccgtccc cggttgaaac 60

gctactgctc agacctctgg ctgtcgcacc tctattaggc aggcaggaac cacccttttg 120

gggcctacgt actctcgccc ttttgggacc cgcggtcgtc atcaccgcgc ctccattagg 180

ctgatagtcg tacctcctct aggctgacaa ctgtcgccct tttagggcct gtgaccttca 240

agttacggct ttagcgtccc tcccgcggcc gactcgccta cgtgaacgct agctcgtcac 300

ccgacggcct acctttcacc cccccccccc ctaaagaacg ttttccgtct tttccgacgt 360

taaaggattg caaccaaacg cttcttaccg tcttttccga cgttaaagga ttgtaaccat 420

aagatacacc ttcctgcagg aagtaaaaca tgcacaaagt aatcagtttt gcccgttttc 480

atgagaaacg ggacgtcagc gcacgaaacg cgccgttgct ctttgcattc ttgtacaaac 540

acgatctaga cgcaggaatc tcagaccaac tcaccccgtg caactgcaag tttcgcccgg 600

cctttccggg tctagagaga caaacagacg cactgaagcc aactccacga ttggtttact 660

agcgggtatt agtaacactc actttgcttc gtagcggagc acatgagcgg tgggaatccc 720

cccatggtaa catggaccca ccgggccaaa agccacgcct cgtgcctcat gtgtgtgcaa 780

ccccagcacg gcaatttgtt tgcgaaacct actttaaggt aacactgaga ctggtacttg 840

acttctggag acaggctaag gatgtccttc aggtacccca aggtaacacg agacacttgg 900

gatctgagaa ggagaccagg agttctttaa aactgcctgg tttaaaaagc ttctatgcct 960

gaataggtga ccggaggccg gcaccttttc ctttaccaca actctatttt 1010

<210> 455

<211> 626

<212> DNA

<213> Duck hepatitis A virus 1 (strain R85952)

<400> 455

tttgaaagcg ggtgcatgca tggccatttt ccagcacagg ttcagctggt atggcatgcg 60

tgcatcgact gcttgggggt gtccctgacc cccttaattc aacgtctagc ccacgatacg 120

ggcaccctat ccaatttgtg tccctttacc tcccatggat tgacttgtat ggatttggat 180

ctaaattgga ttaccctttg tttgtggtgg ttagccaacc atgctctggt gtaatggttc 240

catgtgttca tctggctaag tgccgaccta ggaggtggtg ctgaaatatt gcaagccact 300

agcgttgtta cacttggcct ctggatacgt gggattaatt gcattctgta cagaggaagg 360

ctagtctatg ccttgggcta atggtcttcg ttgtgaaacg gattaccggt agtagcatct 420

tagactacca aggttgtagg tgagtgtgtg gtctagagta ggcacacatc tgtccaggca 480

catgtacatt gttacgattg ctccagacta gttcctgagg gacagatgtt gtggggggct 540

tgggaaaacc cccttaacct acactgcctg atagggtcgc ggctggtcga gtcccacaca 600

ctataatacc agtagacttt catgca 626

<210> 456

<211> 609

<212> DNA

<213> Turkey avisivirus (isolate USA-IN1)

<400> 456

ttgaaagcga atgtatggcc gcatttccac actagtaact agtagtggta tgcggctgtg 60

cattcgcttt ccccccctct ttataggggc ggtctttgta tctttactaa tttcctactt 120

ttctatcatc tctttttaag gtagggacta aggtctccta gcccttcccc ccctgggctt 180

tccaggatca agggggttct aaagctaggc catgtttcct gagcctcatt tagtacggag 240

agttagtagt cataaaacat gccagtaacc ttctagtgcc caagggtgac agagtggtaa 300

cactctcctt ggggcccgaa aggctagtgg gcatagttgt taccaaggaa ggggtcccca 360

ccccaacctg aactaacagc tagaagctca ccttagaaga agtgctggat cccaacatgt 420

cttataatga aagctcaatg gaaaggtgag gagatggaag catgggggtt cactgagtag 480

atgccctgaa ggagaaatct gatcaggggc ccgtgactat acattaggta aaccgggtat 540

aaaaaccatg aaaggtgacc cacaaatttt cctttatttt tatgttttgt tggtgtgaat 600

tcaggcaag 609

<210> 457

<211> 803

<212> DNA

<213> Bopivirus sp (strain bovine/TV-9682/2019-HUN)

<400> 457

ctttcccccc ctccctcccc ccgacgggca actgcaactt ggccccggcg agcgggtagg 60

tcgcactccg gtggatgcta gaatgatcag caggttgcct ctcttgatat cggggctcac 120

ttcggtggac tcttgatgtt tagagatgtc aaagaggatg tcgcagtgtg gcattctcta 180

agcatgcttg tttccatctt tgcatatcca cgtggttggt gtggattcta gttcagctcc 240

cccgcttggg gtcgcttggg ctgaacacca acccatggtt gcagaacgga cagcagcgat 300

gtcagtgacg tttgagggac gattttgggt tccggctttg ccgggctaac gtgccagtgg 360

atcaatacat ggtaacatgt acccctgggg ccgaaagcca agtgttcgca gcacccgtta 420

gcgacttcgg tcgacccatc ttcgcgattc acaccgaact gactagtggc tggtatcgct 480

tctccaacca tgacgttggg ctaaggatgc cctgcaggta cccaattgga tctgagcagg 540

ggccccatcc gtggcgcttt acaccatggc ttggggttaa aaaacgtcta acccaacacg 600

ccagagggat ctggtttcac ttttatttta ttaactcttt ctacactggt gacaagttaa 660

tgataaacgt tgtgcgcctg cagaactatt gacttgactt gttatcacgg tttctacaca 720

gttcaatact ccactttttg tcttaggtgt ttgtcatttc tttgattttt cagttccaat 780

cagcacccac ttaacttttc aca 803

<210> 458

<211> 576

<212> DNA

<213> Encephalomyocarditis virus (ZM12/14)

<400> 458

tccccccctt tctcttcccc ttccttaatt taactggccg aaaccacttg ggataaggcc 60

ggtgtgtgtt tgtctatatg tcacttctac cacaccactg tcttttggtg gtgtgggggc 120

ccagaacctg gccctgtctt cttgacgagc actcctaggg gtctttcccc tcttgccaaa 180

ggagtgtaag gtctgttgga tgtcgcgaag gaagcaatct ctctgtaagc ttctggaaga 240

caagcaacgt ctgtagcgac cctttgcagg cagcggattt ccccacctgg taacaggtgc 300

ctctgcggcc aaaagccacg tgtataacat acacctgcaa aggcggcaca accccagtgc 360

cacgttgtga gttggatagt cgtggaaaga gtttgatggc tttcctcaaa cgtatccagc 420

aaaagggctg aaggatgccc agaaggtacc ccattttatg ggatctgatc tggggccacg 480

gtgcacgtgc tttacacgtg tcgagccggg gttaaaaaac gtctaagccc ttaagtcacg 540

gggacgtggc tttccctgtt aaattaacac tgaaac 576

<210> 459

<211> 955

<212> DNA

<213> Human TMEV-like cardiovirus(NC_010810)

<400> 459

acctgcgaca gtgccaaccc acatctcttc ccactattat tattccggtt cccgccttag 60

gtccattgaa aagaccaaac atgtcatccc ttggatgctg gctaatcaga ggaaagtcag 120

cattttccgg cccaggctaa gagaaacata taagttagaa atgattctaa tcccttgacg 180

aattcggaac gagaagttct ccctcccttg ccgcttgttc acacccatca tttaattcgg 240

ccctttgtga caagcccctc ggtgaaagga cctctctctt tccgacgtgg ttggaattaa 300

catcttttcc gacgaaagtg ctattatgcc tccccgattg tgtgatgctt tctgccctgc 360

tgggcggagc gtcctcgggt tgagaaacct tgaatctttt cctttggagc cttggctccc 420

ccggtctaag ccgcttggaa tatgacaggg ttattttcca aactctttat ttctactttc 480

atgggttcta tccatgaaaa gggtatgtgt tgccccttcc ttctttggag aatctgcgcg 540

gcggtctttc cgtctctcaa caggcgtgga tgcaacatgc cggaaacggt gaagaaaaca 600

gttttctgtg gaaatttaga gtggacatcg aaacagctgt agcgacctca cagtagcagc 660

ggattcccct cttggcgaca agagcctctg cggccaaaag ccccgtggat aagatccact 720

gctgtgagcg gtgcaacccc agcaccctgg ttcgatggcc attctctatg gaaccagaaa 780

atggttttct caagccctcc ggtagagaag ccaagaatgt cctgaaggta ccccgcgcgc 840

gggatctgat caggagacca attggcagtg ctttacgctg ccactttggt ttaaaaactg 900

tcacagcttc tccaaaccaa gtggtcttgg ttttccaatt ttgttgactg acaat 955

<210> 460

<211> 772

<212> DNA

<213> Saffold virus 3 (NGT07-987)

<400> 460

ttcaaaactt gatgttctcc ctcccttgct gcttgatcac acccacctct aaaattcggc 60

cttttgtgac aagccccctg gtgaaaggac ctctcttgtt tcgacgtggt tggaattaac 120

atcaattccg acgaaagtgc tatcatgcct ccccgattat gtgatgtttt ctgccctgct 180

gggcggagca ccctcgggtt gagatacctt gactcttttc ttttggaacc ttggttcccc 240

cggtctaagc cgcttggaat acgacagggt tattttctat actcttactt ctacttttac 300

gggctctatc cgtaaaaagg gtatgtgttg ccccttccat cttcggagaa tccacacggc 360

ggtctttccg tctctcgaaa agtgtggatg caacatgccg gaaacggtga agaaagcagt 420

tttctgtgga aatttagaat ggacatcgaa acagctgtag cgacctcaca gtagcagcgg 480

actcccctct tggtaacaag agcctctgcg gccaaaagcc ccgtggataa gatccactgc 540

tgtgagcggt gcaaccccag caccctggtt cgatggctat tctctatgga accagagaat 600

ggttttctca agccctccgg tagagaagcc aagaatgtcc tgaaggtacc ccgcgtgcgg 660

gatctgatca ggagaccgaa tggtaatgct ttacattatc actttggttt aaaaattgtc 720

acagcttctc caaaccaagt ggtcttggtt ttccttttat ccacatggca ag 772

<210> 461

<211> 1258

<212> DNA

<213> Human cosavirus A (strain AM326/BRA-AM/2017)

<400> 461

ggtcctaccg cgccgctcag gcgcggattg tgtcttccat acacaccttt ccgtgtagcg 60

cacgtaaact gctaggcccg taataggccg actggctttg ccagtggtct ctcccaggag 120

accaaaaccc aactcactta cgagttactt atttatcttg aaactacata aagaacgtgt 180

accgctcttt gtttctttaa ggaaattaga gtagaaaaca tttgaaattg ggctcggttg 240

aactccgggc gctttccatc cgctgtgatg ggctcactcc tgtacaccgt gagtccgcgc 300

agtgctgact taacacttaa gtaatgcata ggccgagaat tacaccgctc ggctcccacc 360

tttttccatc gtgggattaa caggtttaat gcacaaattc ctgtccttgg ctatgtcaaa 420

gcaatacagt gtgtactcgg tgtacacgct caaggcggag atttaggcct cacagattgt 480

gttttgtgtt gttgaatgct ggatggtcac gttggagact gcatgtggca gtcttgaaac 540

gtgtggtttg acgtctatcc attacggcag tgggtggagt actgcaaaga tgtcaccgtg 600

ctttacacgg tttttgaacc ccacaccggc tgtttggcgc ttgcaggaca gcaggtttat 660

tttcttatat tctctatttc tagccaacag ggtactatct tgttgggcgg agtgatactc 720

ccgttccttc ttggacagat tgcctccacg ttctttgtgg atcttgaggt gatcaagtca 780

ctggtaagta gagcgaaggt tgaggagacc tgaggaattt ccatgtggct ttgccaggag 840

ttgtagcgat gctgtgtgtg tgtgcggatc tcccctcatg gtaacatgag cctcacaggc 900

caaaagccct gtccgaaagg acccacacag tggagcaatc ccagctccct cctacaaagc 960

tttgtgagaa tgaactcacg tttattcttc tttattctct acttacatca ggccccaaag 1020

atgtcctgaa ggtaccttgt gtatctgggc acgagcacca tcaactaccc ggacttgtgc 1080

ttcggcgcag acgcatgtgg tgactcagcc cctctgcttc ggcagagggg ctttttgctc 1140

gctcagcacg agatctgatc aggagcccct cccagtgtgc tttacacctg gcgaggggtt 1200

aaaaattgcc caaggcctgg caaaacaacc taggggacta ggttttcctt tattaaca 1258

<210> 462

<211> 1247

<212> DNA

<213> Cosavirus F (strain NGR_2017_NHP_CV)

<400> 462

ggctgctggc acctttccgt gtagcgcacg ttagcgtagg cccgtaatag gctgcatcac 60

cttcttggtg gtgtgctcgg aagagcaaaa cttttcttta accctttaaa aaccttttta 120

ctctagtaac tcgttaagac tttctagaac ccactactac aagatcccat ctgctcagga 180

tgttggtctt tgttccaact agtagtgaga aatttgaata agctttgctg acaaaagaac 240

tagaggtcgg tgtgatgggc tcactcctgt acatcgtgag tatgctcaac cactgactaa 300

agggctgctt gaacttaaca tcatggattc cgatccgtta cattgatcgg cccgtgccct 360

tctcaatatg ggattaacag agcttcaact tggttttcct gtcttttagc gttccaacgg 420

tattatgcca caacatggca gaaagcccgc ggtagctatc acagattgtg taaccaggtt 480

tggctgctgg atggtcatat tcagatcgca tgcatgtgtg gtcttgagat atgtcccggt 540

tgacgtttcc cttatgttac attcttgaca gccggtggag tgctgaaatt acgtcaccat 600

gctttacatg gtttttgaac tccacgccgg tcgctagacg cttgtaaggc gataggctta 660

tcttcctatt tctcttcttt ctagcccagt aggttctatc tactggggcg gagcgaggtt 720

cccgttcctt cttggacagc tggtcaccac accttgtgga ttcttagact ggcaaaacac 780

tggtgcaaga gttgaaggga ttatgtgttt aagtgaagtt ctactgcttt ttgaggaatc 840

gtagcggtgc tgtgtgtgtg tgcggattcc ctcctgcggc gacgcaggcc tcacaggcca 900

aaagccacgg tcgaaagacc ccacacagtg gagatatccc atctccttcc tctcttagta 960

gtttaatgaa tctttttcac aggatcttat aagactactg catttcagac cccaaagatg 1020

gcctgaaggt accttgtgta ttcactttgg ctgaaccacc gactccccgg actggctttg 1080

ccgacgcatg tgggtgccca gcccctgccc ttaagggcag gggcttccct cagcaagtga 1140

cacgagatct gatcaggtgc caggtcactg gtgctttaca ccaccctggt taaaaattgc 1200

ccaaggtctg acaccccaac ccaggggact gggttttctt ttatctt 1247

<210> 463

<211> 982

<212> DNA

<213> Canine picodicistrovirus (strain 209)

<400> 463

agttatggct atgcctttgt gcatatgctc gatgtctctc ccttttatga ccttttcccg 60

accaacatct catgtcggat taaactggat gatcttttaa gactccctaa cccagaattc 120

ctccggggta ctcctgtgga tctagcttat actggattcc cgcccggcgt tgttcgatct 180

gaacggcgtt agtttcacct gtggtagcct ctgcggaggg cccacggcgt ttagtggttc 240

tgcctaagga caaccaccta acctacccat gtctctatcg tccctactag ttttaggcag 300

tattgtacgc tctttataca aatctgtcaa ccctttgtat aactctaagc cgaacaatta 360

tagctaggct ttttattata taacattaat taggcattag cgttgtcgcc aatctcttgg 420

taatcctaag gatacctttc ctgttgacta agatgaagcg ccttcggtta ccgatgcccg 480

gtgtccacga agccatcgtg gtcggccgcg tcccccacct ctcccaactt ggactccatg 540

ttttcagtag gtgtaatgat tagtattatt gattctgctc gttcaatgtg tttatcttca 600

cgatctggga cccaacacat gcttcactca tgtttaaatg ttggttccct cattttgaag 660

acacccaaac catagagtgc gagaatgagg atttctactt ccattctggt aacagaaatg 720

aattcctgcg tgtgtctcgt aaatggaatc tttaagaact tcagataaat cgaacaatac 780

actaatacaa gttgttttct accaacatgt tcaatgcggc taatctgacc gtggagctgt 840

gaagcgctca aacccgagtg ttgtatacag tcgtaatgcg taagtccatg aggaaccgac 900

tactgttacc tctgttggtg tgtttctcct ttattctctt ttattattat ttgttattgc 960

aaatactaca actttgatca ac 982

<210> 464

<211> 894

<212> DNA

<213> Equine rhinitis B virus 1

<400> 464

ttttgtaact gaggttttga catctcattg tttttgagcc ccaacgtctt ggtggatgct 60

caaagaagat tactcttgat gcttggctct cagaaagaat ggcacctcaa tgttttgaga 120

cccgattctt ttgagtcgag accatttccc cttccctgaa gattgctttt cttccaacta 180

aacccctatt gatgtgttgg tcgtttgcca atcggagggc gacaggcaca ggtcgctccg 240

agttctagta gtgtgggaac ttgttactac tgatgaaacg aggtagtgac actcagtacc 300

tgcgaacgag gtcggggccc tcccttcttc cttcacccaa ctttcacttt tcgttccact 360

ttagcagggg tcttctttct atccccctgg cggcattgga actagccgtc gcgtcttaac 420

gcgcagccct gaaggcccca caccttgtgg atcttgccgt gggtatgttt ctggcatgtg 480

tttctcaagc ctgcaaccga agccgaacag ccacatgaac agtttgagcg tggtagcgct 540

gtgtgagttg gcggtggatc cccctcgtgg taacacgagc ccccgtggcc aaaagcccag 600

tgtttacagc acctctcaca tccaggacga ccccatcctg gcgctcactc ttagtagtat 660

ggcttagtac gcattaggtg gtaagccgag ctctccctcg gccttgttct gaatgcacac 720

atgtctaggg gctaaggatg tcctacaggt acccgcacgt aaccttcaga gagtgcggat 780

ctgagtagga gaccgtggtg cactgcttta cagatgcagc ccggtttaaa aagcgtctat 840

gcccctacag ggtagcggtg ggccgcgccc tttcctttta aaactacttg ttct 894

<210> 465

<211> 669

<212> DNA

<213> Simian hepatitis A virus

<400> 465

cttgatacct caccgccgtt tgcctaggct ataggcttct tccctacacc cttgtttgtc 60

tttttttttt ttttttgtgt gtttgtaaat attaattcct gcaggttcag ggttcttaat 120

ttgttctgct atacagacac tcttttcacg ctttctgtca tcttatttcc tgggctctcc 180

ccttgcccaa ggctctggcc gttgcgcccg gcggggtcaa ctccatggtt agcatggagc 240

tgtaggagtc taaattgggg acgcagatgc taggaacgtc gccctgcagt gttaacctgg 300

ctttcatgaa gctctttgat cttctacaag aggtaggcta cgggtgaaac ctcttagatt 360

aatactccta tggagagata tcttgaatag ggtaacagcg gtggatattg gtgagttcct 420

ttgggacaaa aaccattcaa caccggagga ctgactctca ttcagtagtt gcattgagtg 480

aattgtctgt cagggctgtc tttgggttta attcctggcc tctctgtgct tagggcaaac 540

catttcctgg ccttaaatgg agttctgtga gagggaactc ctcctttata tgctggacat 600

attttggggc cttagggtta tggtttgcct ctgaggtact caggggcatt taggtttttc 660

ctcatttat 669

<210> 466

<211> 708

<212> DNA

<213> Hepatovirus D2 (isolate KS111230Crimig2011)

<400> 466

ttcaaaagag ggtggggagt tttctcccca cccccttttg gatacccatg gtgcgggggt 60

ataaattacc gcaccccctt tgctgtgccc agggtatggg cacctttctg tctgttttgt 120

ttcgtttgtc tgctttatgt gtgctcgtcc cctatagggc actcatttca gctttctttc 180

attctttctt ttccccggtt ctcaccttac cggaggcact ggccgttgcg cccggcgggg 240

tcaacctagt gattagcact aggctgtagg tgtctaaagt ggtgacacaa agaggtgttt 300

cctgattcca gcactgttaa ctgatacaga tgaaacctga ttgatcttct agaaggggta 360

ggctacgggt gaaacccctt atcttaatac tactatgtag agatagatcc agtaggttaa 420

gggcagtgga taaggttgag ttcaaatgga caataaatct tcaacactgg tggacccaat 480

ctcactggct agttgccttc agtgtggatt ctgtggaggg gtgtttcttc tggataggga 540

accatcacac tggtgcctga gactcagggt gtctggcctt aaatcacaga agccgtatgg 600

tttttgtggc ctagcttctg cctctaaaca gactctgggg cccaatgaca ccctgagccc 660

ttcggggtac tcaggggcat ttaggttttc cacatttcaa aatagtca 708

<210> 467

<211> 711

<212> DNA

<213> Rodent hepatovirus (KEF121Sigmas2012)

<400> 467

ttcaaagggt ggctgggatt ttgtcccagc ctccctttgg agttccttgg ctctggggaa 60

ctaaattacc aggcccgcgt ttacctagcg cttaggttaa aacccccatc ccttatttgt 120

aaatattgat ttctgcaggt tcagagtttc ttaatcatgt tccctttagg acactcactc 180

cacttgcttt cattcttgtt tccgtggttc tcccatcacc acaagcactg accattgcgc 240

ccggtggggt taaccatatg attagcatat ggctgtaggt gtttaagggt gggacaataa 300

tggaaggtca aatagcaggc ttgtttaact tccttttgtt tcatctcatg tatctttctg 360

aaggggtagg ctacgggtga aaccccttag gttaatactc atattgagag atacctgtgg 420

taggttaatt gccctggata aacttgagtt gttagacaat aaagtttcaa caggggtgga 480

caagatctca ccaccagact ggcatgttgt gggacatctg ggaggcaagt ttcttcctgt 540

gatgttggcc tttacatatt gcctgggtgt taagaaggag cccttaaatt cacttttctg 600

tgagcctagc ttccgctgga cggactttgg gggcagcatc cttcttgatg cttctggggt 660

actcaggggc atttaggttt ttccacatta taaatatata gatgaataga g 711

<210> 468

<211> 424

<212> DNA

<213> Hepatovirus G2 (isolate FO1AF48Rhilan2010)

<400> 468

gtcttctttt atccgggctc acgtgatgcc aatggtccga cccatgcgcc cgtgggggtt 60

aacacatgga ttagccatgt gctgtaggcc ctaaaagtca cgttcacgta aggcttggat 120

gaaactcatg tctgttaact gagggtggta agttacattg atcttctaaa gagggtaggc 180

tacgggtgaa accctctagg ttagtactca tattgagaga tacctccagt aggtgaaggt 240

ttccggatat tggtgagttt aacgacaata accattcaac ggatgtgggc ccacctcact 300

ggttagatgc cttgtaatcc aacacccaag gggatatgag tgaattctta gtcccaagtg 360

accctcccga aaggttgtag gggtactcag gggcatttag gttttccaca tttaaagtga 420

attg 424

<210> 469

<211> 683

<212> DNA

<213> Loch Leven virus (isolate MW12_1o)

<400> 469

gttgacgcgc aggagccttt ggacccaagc cctggattgg gctcgttttg gcggttttcg 60

ttaaaactgt cctagtctca tacgagctag tcaatatcgt atgcgtaggc ggagcgttat 120

gccgccaatt tgtttccttt tcccttttat aaccccatct tttcccaacc ctagtgtgtg 180

agttttaata gctcttgctg cttttaaatc tttgcttgct ttgttggtcg gtagatagga 240

gtttcctctt tatatatatg tatctatatt cccggctgtc tccatgccta gagctgctac 300

cgctgcgccc ggcggggtta actccacgat tagcgtggag ctgtggccgc caaaagtgga 360

gatgcagtga ctaattgtgt ttgttagttc catgacttgg gggaggagcg tatggcgttc 420

gatcggtcca aatgaggtag gctacgggtg aaacctcatg gctcttctaa atactatgta 480

tgatggggtt gtggtacgct aacctagacg gataaaggtg agatgctcgt cgtcaaaaac 540

ctttcaacgt ctggccgggg gcgtcacact tggagcgcga acgacccagc aaaccaaact 600

ttcttgttgt ggagccttct aacacataag ctgtacccta tgcgtttagg gctactcagg 660

ggcatgttag gttttaggcc aag 683

<210> 470

<211> 601

<212> DNA

<213> Hunnivirus 05VZ (isolate 05VZ-75-RAT099)

<400> 470

agctttccta cccttcattt gtgaacccac tagtccaagc cgcttggaat acgatgagtg 60

gaaatgttca ttctaaatgg agtgaaacat gcttaatttt ctagtccatg ctggtctttc 120

cagtgtggag cggccctgtc tggccgtaat tctccagagc gtcacgccat acttgtggac 180

ctcacgtgcc gcatgacagc gctgcagctg gaactgggtg cgcggtgccc atagagtaac 240

agcgaaaagt gttagatcaa gctttgcttg ggctatgagc ctgcggaata acaactggta 300

acagttgcct caggggccga aagccacggt gttaacagca ccctcatagt ttgatccacc 360

ccagggtggt gatgtttaac agttagtagt tgccaatctg tgttcactga aatctcggca 420

taccgtgcag tgtacagggg tgaaggatgc ccagaaggta cccgtaggta accttaagag 480

actatggatc tgatctgggg ccttgtccgg agtgctttac acacggctca aggttaaaaa 540

acgtctagcc ccacagagcc cgagggattc gggttttccc ttaaaaaccc gactagagct 600

t 601

<210> 471

<211> 642

<212> DNA

<213> Melegrivirus A(NC_023858)

<400> 471

ttcccgaccg gtctggcaaa ccggacggtt atcctggtta gatgtctgat ggttgctgga 60

acgtggtggc tattgctgcc atcttctggc tgcctttatt gggcattcta gctgggttct 120

ttgccaaaat tcccatctta ttcttattcc cattttcctt acccaggctt atttacaact 180

ggtaagttga cccactggtt ttgttcttgg acttctctgg tgttggtgga cactctttcc 240

acaagtaagt attgtggagt tcatgctcgt tctgaaccgg gaatggcaca acccgtagta 300

ggatcttgcc tctgccacat ttacttgcgc ctgttgcttc tagactatgg gctgctaagg 360

atgaccgcag accccttttt gggcaatctg tgtcaagtct actgcttcgt ctggtgtacg 420

ctggaaatcc ctgttctgga tgcctatgct tggaggtggt gctgaaatat tgcaagccac 480

aagcatacct ctggactcgg tgccgctacc aggtgttgca ccccttgtgg gcaaacgtag 540

aaccttggtg gacgcgtgag atgggaatcc acgcctggcc atagactggc tgataagctc 600

gcggctgatc gagttgcaac agtaatcagt tgatttgcca ct 642

<210> 472

<211> 668

<212> DNA

<213> Canine picornavirus

<400> 472

ttatttttca aaatgccact ggcattacac catattcgta tggtgtaatg tcagtggttg 60

gatgcccagt cctgagggac tgtgcatcca gtggttaaga gccacactct ttatttatca 120

actattgatc tttgtaacta agaaatgtac cagagttcta ttatggctcc aaacccattg 180

gggtctagaa caagcacttc tgttaccccg gtcattattg gctgttagga tgtacccacg 240

tctgaaggcc aagaagatcg ttacccggct gttgtctacg ggaagactag taacttttgt 300

tgaaaggtaa tctcattgat ccgggtggca accccagtca cccagaaact agtgatgagt 360

gtccacattc ctcctatggt aacatagggt ggtcgctgcg ctggcactag ggccttaagg 420

catgagagaa ccaagacaca actgtgaaga tccgactgtg cacttcagta ttcctccggc 480

ccctgaatgc ggctaatcct aacccacttg cacacgctcg caacccagtg agtggtgtgt 540

cgtaatgcgc aagaagtgga cggaaccgac tactttgggt gtccgtgttt ctttttttat 600

ttaccaagtc tcttatggtc acaagcatta gaaacaagat gcctgtgatc actcgcgaga 660

cctccaac 668

<210> 473

<211> 646

<212> DNA

<213> Turdivirus 3

<400> 473

gaaagggggc ccggcggggc aacacccgtc ggtccctctt tccgtccaag ccgcggacgt 60

taaactgtgg cacggttgtg cctaggtgca accctgctac taatggcggt acccctgccc 120

ttagtatgca tgtttgtatg aatggaatct gtagtgatgc cctgctttgg ggtctctaaa 180

acacactaca gtgatggcta gagctcctct actccggcta ccttagtagt gagcattgga 240

agtatattgt gaacagggtt ccacgcccga taaaggctgg tattcccgct catggagtgg 300

acctaggtaa ctagggccat gagtgtgtgt gaatcagcac ccgcaaggga tagcagtata 360

tggaaggtgc tcgtgaaacg gcaagtggag ttagtagcag ctctcagtct caaggtcagc 420

ttctagacat gtgccgcagg taccccgtaa gggatctgag cggtagctaa ttagtccctg 480

gaggttggct gactttcttc ctatttctct tttcactgcg aattgtgtgg attggtattc 540

tttagagctt gggagttgtc tcatattgaa ttcatatatt ttactctata ctacactcaa 600

acacggctct ctggtcagtt aagaaaatca tttatagaat aataaa 646

<210> 474

<211> 415

<212> DNA

<213> Pasivirus A3 (strain swine/Zsana1/2013/HUN)

<400> 474

tcctcaccca tgcttttcct acccccacca cgcccgcatg tttactgctt tccttgatgc 60

tgcccgtgac tacttccatg acctccccaa cccaaacctc aaacgcctta agctatcatg 120

ggctctctat caccaatccc cttccttccc acccagaacc cccccctccc tcccctacaa 180

cgtgtatgag caagacgagt ttgacaagct cgccgaagcc atgctcaccc actctccctt 240

ccccacatcc cattacctct tccaccccct cccgtccaac gcccgcacca tcgtcgagcg 300

tgaggctgac tgggatggct gtgatctaga aaagaaatgg ctcgacctcg tcatagagga 360

cgatgccaag ttccttctgg agaacggctc tctcccgttt ggctccaccc ttgcc 415

<210> 475

<211> 807

<212> DNA

<213> Passerivirus (sp. strain waxbill/DB01/HUN/2014)

<400> 475

ttgaaagccc cacctggggg gctttccctg tgctgcgacc tgtcgctaac caagaggcat 60

gacctcaaga ttggacccac agtcgccaca ggtgtccgtt gatacgctcc ggacctaacc 120

cggataatgt aacatcaaat tatttaagat ttatgagctt atatactgtt atgaacacca 180

gtaaacatgt tagattaaga gcaaaacacc cccctcgtca attctagaag tccgcgaggg 240

atttagggta gcaaactagg aatctagatg catattcttg ttttaaaacc cacgctgcaa 300

gtgatctgtt cactctcatc caccctcagg gtggtcagtg tggtgagtca tagcgacacc 360

taacagaggc taacctagta actcttgtga agttcaacac gctgacagga ttccacgtcc 420

gtttactgct agcattcccg ctcagagggt ggcccaagta attggtgctc tgagtgtgcg 480

tgagctagta tcccgcgagg gataggtgtg ttgtaatgac acaagttggt gaaaggtaaa 540

gtgctttagg ctagctactg gcccattggc tacgagtaga catgtgcagg aggtacccgc 600

tcatcttagc ggatgtgacc tgtagctaat tagtcaatgc tcgctgtaga aatcttttcc 660

ccttttttct tttatataca gagtgattcg catttgtatt gaagattgtg tgcctagtgt 720

atcttggtat tatcagagga agcaatttaa actggatttt caattaggag gattaaaacc 780

ttgttaactt tttataagca ttataaa 807

<210> 476

<211> 746

<212> DNA

<213> Wenling sharpspine skate picornavirus (strain DHBYCGS18742)

<400> 476

tccgttatag gattatttgg acctctatat tgattgacga gaggttaaac aatgtgcgtt 60

tgcttcatga tttattatgt tgtgatcgtt ggtcttctta acacattgat gtcaatcacc 120

cttttacttt actttgtaac taagtttcat acatttcttt gaaattctca tttacaatat 180

tgtcgagctg attcacatgt ttcatttact tttactacaa atctaaatgt tccacaatca 240

tattttgttt attcataatc aaggttgtaa gtattatgca gggcaaccca cgcagtcaat 300

tggtcactca tgaagtggct gtggattaac gcctgaatgt atttgcttat taatatcttg 360

ttttacgaat agataaatta tgtggtcttt ttaaactacc cttgttgtta attgggtcga 420

atcatcgcac acataatgct attttagaca aacgttgttc gcgagtatgc acggtattgg 480

accattattg cttgtcatta gcaccattgg taacaatgat gacaatcccc ggttttcact 540

taagacagct ccgcgcttcg tgctgtgttt gagccttgag tgttgacgtc tgggatgggt 600

ggttgtgctc cactttatct agactgtgta tataagttag caagctgttg ctttcttagc 660

attatgtggt gggcccgacc acaaagggcg gtgggatgtg ccaccgtaaa taatcatatc 720

aaaacccttt attttaatat gtagac 746

<210> 477

<211> 667

<212> DNA

<213> Picornaviridae (sp. rodent/RL/PicoV/FJ2015)

<400> 477

gggtttctct gccctgtgct ttggacgccc gccatggcgt taaactggca atcccccctt 60

ttccctaccc gtaactaagt ttttcctcgg tcctttaggc tgctaagacg cttgttgcta 120

cagcatattg gaaccaccga gtcctccata agtaatcatt tccacctctt tgggtgtcta 180

tgcccttaga gacggtttgg agccccgttg actatgtcag agtgtgtcgg tagcaggctt 240

tctgctctgg acccatgccg gccacacctc ccagtagtgt tgtgaaggaa gtaagggatt 300

agtgaaaaga ttaggaatgt tgccttggaa tgtagcgatt actgtaggtg agcggacttc 360

cccgggtggt aacacccgcc tctcaggcca aaagccacgg tgttaacagc acccctacag 420

taggccacta ccccgtggtc tccaagaata cagacctctt cttagtaact atccccctag 480

ctttcctgaa gcatgctgct gtaaaggcta aggatgccct gaaggtaccc gcaggtaacg 540

ttaagacact gtggatctga tcaggggctt gagtactgaa gctttacaga agtagctcaa 600

gttaaaaaac gtctatgcct ttcccccacg ggagtggggg ttcccccaca caatttcaac 660

aggcact 667

<210> 478

<211> 425

<212> DNA

<213> Avian sapelovirus

<400> 478

ccccccccca taccttttcc ccctctttaa cggcaatggt taatggacta aggataaagg 60

attatggact tatatgatgt gcccgacatg gaattcggga aatctggatc taagggttac 120

ctcaaaggta gtgaaattcc ctcgcagggg tatagtagct agctttgtgc cttagtgaag 180

ggaccgtgtg ttctagacat ggtagttgtg taggtacaag aggacttcat ttgaatatcc 240

tcgatcccgg acctaaatta ccttgtggat ttccggagga atttaggaaa ccacctcctt 300

cgggaggcta gtaggagctt acatgactct cggattggga agtccgagct ggaccatgac 360

tgggtgatag cctgccggct ggcgcagttc acatggtata gtcagttgaa aataaataga 420

aaatc 425

<210> 479

<211> 679

<212> DNA

<213> Marmot sapelovirus 2 (strain HT6)

<400> 479

gggaaatacg gggggggatt acccccctcc cgtatttccc cctgttgccc gggggtagtg 60

aacgatggat ctacggatcc tttgttcaca gggccaaggt tcaagcccac ttttgtgaga 120

agcatgcttt actaggctat gggttgagtg gcccataggc aaccctatga agaggctaca 180

ctcactacat ccgccaacag gcccaggcgt atgtgggcct ataagaaatg tacgttcgtc 240

cgcttatggt gcctaatcca taggcatcta ggacaagtac ttctgtttcc ccggtcacga 300

ggaatatgct gtacccacgg cagaagccag acatgatcgt tacccgactc cgaactgtgc 360

gaaacctagt accttcaagt gaaacagaga tcattgatcc ggggtgaccc tgaccccaga 420

aactcatgat gggtgtcgcc gttccgacag gggagaccct gggcgtcgcc gcgctggcat 480

gagggcccat tagggcatga tcaatgcgga ccttgaggcg aagagtctag tgcgcttcgg 540

tttattcctc caggcccctg aatgcggcta atcctaaccc cggagcgtat cacaccaatc 600

caggtgctgg tgcgtcgtaa tgcgtaagtc tgggatggaa ccgactactt tgggtgtcct 660

gtgtttctaa tttctcttg 679

<210> 480

<211> 867

<212> DNA

<213> Bat picornavirus (isolate BtPV/13585-58/M.dau/DK/2014)

<400> 480

attttgtact cctcaatcac acgaaaggcg tgggatcaaa gaacgacggg ttgacgtcgc 60

tacatacttg aaaaattgca acaaactctg tcgataggca cactgtgtta tatgctactt 120

tagtaatgat cttagatgga atgctctctt ctttttgtaa tttattgcaa ctggaatgat 180

ctttcttatt tgaccccaga ctgatttagt aggcgacctt tgtgtggagc taccccaaaa 240

ccctcttata tgtattattt tatgttattg tacgtaccac tcattattag actataaacg 300

tcgtgagtaa aaatcaaaaa cagtgaaatg ttctacatat gcggattatt ggagcgacaa 360

tttgactagt tgactaaagt accttgtgag ataacttaca taattctttt taatgtgtta 420

agtattacca gttggcctaa gcccccaaca aataaattaa catgttgaac tgatggtaag 480

cctcgcttgt ggaaacagta gcttttgttt tgaccattgt tcgagaaatc ctcttaggga 540

cctccctaag accgtgcaac agacaggtaa caacggacta gagaattcac ctctaggcta 600

cctgtaagca cgttcaatgg tataaacata agtgcaaaag actaacagat ttgtttcaat 660

ttttcaagta tgtagcgacg tcaacccgtc gttctttgat cccacgcctt tcgtgtgatt 720

gaggagtaca aaataggttg ggttggtcac caaagaccca ttaccagtaa ggaatactgt 780

gttgacggca accaaaaagg ttgtggctgc ttgtactaaa gctgaccgtt tgaaagtaaa 840

cacccccccc ccctgcttat accctat 867

<210> 481

<211> 526

<212> DNA

<213> Bat picornavirus LMA6 (isolate DesRot/Peru/LMA6_F_DrPicoV)

<400> 481

acggttgcgc aagatggaat tgcgaccact actgtggctt tctctgactt ggatagttaa 60

ccctgtaccg ctgggtgaaa agggcccctc gcgttgcata tgtgagcaag gaggtactaa 120

ataacttctt gtttgcatgt gtgaacgcga gggatcaccc ttttcaccct gcggtacagg 180

gttaactatc caagtcagag aaagccacag tagtggtcgc aattccatct tgcgcaaccg 240

taaagcttta gtgtaaaact gggcgccgaa ctggatctcg acaatccata gtaggagtgc 300

agcctcttac acgaaaaccg aaggagagac gagtgcacac caagtgtagt aattccatga 360

gtacatggag ccccagacac gtggtacgcg tagcctacgt ggtgtcgttg ggggtgggca 420

tgccctctac aactggtgac cggagcgggg gacgccgagg ttttatgcac acgacctgat 480

agggtgtggg ccgacactct ctcccgtatt cggctgtaca taagcc 526

<210> 482

<211> 939

<212> DNA

<213> Sicinivirus A1 (isolate JSY)

<400> 482

ggggggcttt ccgggtgggg tggatcctgt cgcgtgcctt ccgccgcaca gtgtccaccc 60

ctcctgctac ccgctctgag tagcggtacc ccccattccc tccctttccc cccacatggc 120

tggagcggcc cacttgcctt cgggctcgtg tgccaacccc aacccggggt cgtggcggac 180

gtcatggctc cggccatgac atcctaacca ctccttaaac ccaccttatt ttattttgaa 240

atttaagttt aaattccccc taacccaaag gccttggccc atgtccttct aaggttagat 300

ggcatggaca ctctttgttc agcgtccgtc gacccactgg gctaccactt tttcactcta 360

aacacaactc cttttagtaa ccaccctggc tttgtcgtgg ttctgctcct gcagcacacg 420

agtgcccaag gatcacggct ggtaacagtt gccttggggc ccgaaaggct aggtggcaag 480

ggtgtggtca agtgaggggt tcccaccccg agccgactcc acattcctga agccaccctt 540

cgtgtggtag gccaatggct tcgatcgcga ccttgtcagt gtgttgaaac gagctagaag 600

tgtttagaag ttggattagt tggtttgtgg tgattagtag accccctgga ggtacccaat 660

cttggatctg accagggacc cgtgactata ccgttccggt aattcgggtt taaaataatg 720

aacgtcacca cccaatttac tctctctact attcattttc ctaataccac tactcacgga 780

tgattgtgga ggttcttctg gttgttattg tcttggctat tgtcatttcc tcgcctaacg 840

aactcaccca gctattggct atttgcattt acaattttgt cctgatcttt ttgtggttgt 900

ttggtattct agaactcccc aactacccca cagttcggc 939

<210> 483

<211> 370

<212> DNA

<213> Sicinivirus A5 (strain RS/BR/2015/1)

<400> 483

cccgattgct gtcctgcttg tctggtgaac tcggctggaa gcattcagtt tctggatttg 60

ctggtttgtg gtgattagta gaccccctgg aggtacccgt ctggatctga ccagggaccc 120

gtgactatac cgttccggta attcgggttt aaaactatga acgtcaccac ccattttgtt 180

ttgtttggtt gggctggatt gtttgtttag atgctcctag aatccctgtt ggtgattgta 240

gcacttgttg tagtggtcgt ctctcctagt gaattacccc agctgtttgc tatttgcatc 300

tacaactttg tcttgatttt tctctggtta tttggtacct tagagctccc caattatccc 360

accattcatc 370

<210> 484

<211> 949

<212> DNA

<213> Sicinivirus (sp. isolate Environment/NLD/2019/VE_7_picorna_3)

<400> 484

ggctcatgtc ctttgaggta agacgacata gacacccttt gtctagcgtt cttcgagcca 60

ctgagccacc acttcttcgc tccaaacccg actcctttta gtaaccgcat tcccttccct 120

atccccccat acagccggaa cggaccactc acctccgggt ttgtggccca accccgactt 180

ggggttgtgg cggtggtcac agctccggct gtgacttcct aaccactcct tacacccacc 240

cgttgcattt gctaacttaa gttttctatc cccatcccta aggccttggc tcatgtcctt 300

tgaggtaaga cgacatagac accctttgtc tagcgttctt cgagccactg agccaccact 360

tcttcgctcc aaacccgact ccttttagta accgtactga cattattgtt gttctgccta 420

cgcagcatgc gagtgcccaa ggatcacgat tggtgacaat cgccttgggg cccgaaaggc 480

taggtggtaa ggatgtggtc aagtgagggg cccccgcccc gagccgactc cgcactcccg 540

aagccaccct tcgcatggtg gatgatttcc cgattgcaat tatgccagtg cgttgaaaca 600

agctagaagt gtttggaagt cgaattagct ggtttgtggt gattagtaga ccccctggag 660

gtacccattt tggatctgac cagggacccg tgactatacc gatccggtaa tccgggttta 720

aaacaatgaa cgtcaccacc caattacttt cctaatttct taactttaat gttgttaact 780

ggtgttcaga tgcttttaga gattttcctt gttgtcttgg cgctaattat tgtgattgtg 840

acacctggcg aactatctca attccttgct atttgtgtct acaattttat tttgattttc 900

ttttggctat ttggattatt cgaactccct gattacccca ccatccaac 949

<210> 485

<211> 331

<212> DNA

<213> Porcine teschovirus 10 (strain Vir 460/88)

<400> 485

actagacttc ggtctcttgt tgtgtttaaa cacagaaatc ttgctacttg gttatgaatt 60

cattggatca acccctctga aagacctgct ctggtgcgag caaaagcgca attgtcacca 120

ggtattgcac caatggtggc gacagggtac agaagagcaa gtactcctga ctgggtaatg 180

ggactgcatt gcatatccct aggcacctat tgagatttct ctggggccca ccagcgtgga 240

gttcctgtac gggaatgcag gactggactt gtgctgcctg acagggtcgc ggctggccgt 300

ctgtactttg tatagtcagt tgaaactcac c 331

<210> 486

<211> 493

<212> DNA

<213> Tremovirus A (isolate GDs29)

<400> 486

tttgaaagag gcctccgggg aatccggagg ttctctttcg acccaaccca tactgggggg 60

tgtgtggggc cgtatttgga gtgcacggta tatatgcatt cccgcatggc aagggcgtgc 120

caccttgccc cttgacgcat ggtatgtgtc atcatttgcc ttggttaaac cccataaaac 180

gaggcgccac gtgccgaaaa tccctttgcg tttcacagaa ccatcctaac catgggtgta 240

gtatgggaat cgtgtatggg ggtgattagg atctctcgta gagggatagg tgtgtcattc 300

aaatccaggg agtactctgg ctctgacatt gggacatttg atgtaaccgg acctggttca 360

gtatccgggt tgtcctgtat tgttacggtg tatccgtctt gacacactga aagggtattc 420

ttgggtaatc ccttcctact gcctgatagg gcggcgtgcc cggccacgag agattaaggg 480

tagcaattta aac 493

<210> 487

<211> 991

<212> DNA

<213> Yili teratoscincus roborowskii picornavirus 1 (strain LPWC175499)

<400> 487

ccggggggaa tccccggtaa tccacttttc ggccagcgtc tgttgctggc actctcgccc 60

aatcttataa cactaggtat tgggtcttac ccgaaaggag aatcccacta caatagatta 120

taatgataaa gagatagatc gacggttgcg ggtcttacct taaccgggaa tcccaaaaca 180

agcgtctata ctccagagag agatcaaata agcagcgtaa tggtgatacg ttaaacaatc 240

cgtcatgaga agacgtaaaa atccttgtac tgcaagtaaa attgcaactt ttgtaattaa 300

gtaaatatgt acctcacaca tgtgagcact ctctaacatc agagaggaaa taatcttaaa 360

ttgtaatacg tgttctggtg aaccgtttag aaaccacgtc cgactagaga gactagtcca 420

cggagaaatc cagatgttca agttcactac gctttcctgc ggaaacaatg gttagctgag 480

atttgaacgg gcgtttaagt tctagacacc tcacagtgga gcgatccaca acagagctac 540

aatcttcagg gctaactctg gtgcgccgag gcatagcggc aactgtgtat taggagcatt 600

tcagggcttg aagagcaggc acgtgcttaa aatgtagcaa aactcaagat gttgagcaat 660

gcgttctgtt tgaaaaccat gagtacatgg taacctacca cattgaccta tagtgcataa 720

cgagtgaaga tgtagtttga aaacatgcag cggatcatgg aattgatcgc ggtgtttcag 780

atgaaatttt cattgcagtg aacattatag agggctcgtt tttcgataac cctttgtcag 840

ataatgttgg aggtgggtac acccacttac agaaggtacc gttttcgtgt gggttgcgaa 900

aatggcgtta ttagttaaca cctaataatg ttgtgcaggg tgacagcctg tcggcgggtg 960

cacatcttgt tttaatccgt tgtcttgcca t 991

<210> 488

<211> 712

<212> DNA

<213> Canine kobuvirus (US-PC0082)

<400> 488

tcacgcactt ttccggtggt ccggacccag accaccgtca ctccattcag cttcttcgga 60

acctgttcgg aggaattaaa tgggcaccca ctcacccttc ccctcttctc gtaactaagt 120

gtgtgcccaa tctcttgact cctactggct ccaccgaccg gtagtgtcca aaatgctagg 180

tggacttcct cccttccctt ctgggcatct tgcccagctt cctcccccca gcctgacgtg 240

tcacaggctg tgcaaagacc ccgcgaaagc tgccaaaagt ggcaattgtg ggtcccccct 300

ttgtcaaggc gtcgagtctt tctcccttaa ggctagtccc atcagtgaat tctgatgggc 360

aactagtgac gccactgcat gcctccgacc tcggccgcgg agtgctgccc cccaagtcgt 420

gcccctgacc acaagttgtg ctgtctggca aacattgtct gtgagaatgt tccgctgtgg 480

ctgccaagcc tggtaacagg ctgccccagt gtgcgtagtt ctcatccaga cttcggtctg 540

gcaacttgtt gttaagacac ggcgtaaggg gcgtgtgcca acgcccagga acgagtgtcc 600

actctaatac cccgaggaat gctacgcagg tacccctggt ccaccaggga tctgagcgta 660

ggctaactgt ctaagggtat tttcatttcc cactctctct ttctcgttca ta 712

<210> 489

<211> 646

<212> DNA

<213> Feline kobuvirus (strain FK-13)

<400> 489

ggaattaaac gggcacccac tcacccccat ccccccctgt aacttaaagt gtgtgctcgt 60

gaccttgact cctgcgggct tcaccgaccc gtccagtgaa caaactgcta ggtgactcct 120

tccccccccc tggcctggat ggcccacatc caggtcttct tcccccagcc cgacatgtca 180

tgggctgttt ggaatccccg cgaaagctgc taacgtggca attgtgggtc ccccttccaa 240

caagacaccg agtctttctc ccttaaggct agccccgtgt ggtgaattcc accgggcaac 300

tagtggtgtc aatgcatgcc tccgacctcg gccgcggagt gctgcccccc aagttgcgaa 360

actgaccaag actgtgctgc ctggcaagca tagtctgagg agatgttccg ctgtggttgc 420

caaacctggt aacaggtgcc ccagtgtgcg tagtctcctt ccggttcgtc cggctgtcct 480

ttggtaaaga cacaatgtaa ggggcgtgtg ccaacgccct ggaaagagtg aacttctact 540

gccctaggaa tgccacgcag gtacccctgg tttcccacca gggatctgag cgtgggctaa 600

ttgtctaacg ggtagtttca tttccctcct ctcttttgtt gacatc 646

<210> 490

<211> 647

<212> DNA

<213> Feline kobuvirus (strain WHJ-1)

<400> 490

ggaattaaac gggcacccac tcatccctac ctccccctgt aacttgaagt gtgtgctcgt 60

gaccttgact cctgcgggct tcaccgaccc gtccagtgaa caaactgcta ggtgatttct 120

tccccttccc ccacctggat gttctacatc caggtcttcc tcccccagcc cgacatgtca 180

tgggctgttt ggaacccccg cgaaagctgc taacgtggca attgtgggtc ccccttccaa 240

caagacaccg agtctttctc ccttaaggct agccccgtgt ggtgaattcc accgggcaac 300

tagtggtgtc aatgcatgcc tccgacctcg gccgcggagt gctgcccccc aagttgtgaa 360

actgaccaag gattgtgctg cctggcaagc atagtctgag gagatgttcc gctgtggctg 420

ccaaacctgg taacaggtgc cccagtgcgc gtagtctcct tccggttcgt ccggctgtcc 480

tctggtaaag acacaatgta aggggtgtgt gccaacgccc tggaaagagt gaacttctac 540

tgccctagga atgccacgca ggtacccctg gtcccccacc agggatctga gcgtgggcta 600

attgtctaac gggtagtttc atttctctcc tctcctttgt tgacatc 647

<210> 491

<211> 561

<212> DNA

<213> Kobuvirus (dog/AN211D/USA/2009)

<400> 491

ccgaccagta gtgtccgaaa tgctagagtg gacttcctcc cttcccctct gggtatcttg 60

cccagtttcc tccccccaac ctgacgtgcc acaggctgtg aaaacacccc gcgaaagctg 120

ccaaaagtgg gaattgtggg tccccccctt tgccaaggag tcgagtcttt ctcccttaag 180

gctagcccca tcagtgaatt ctgttgggca actagtgacg ccactgcatg cctccgacct 240

cggccgcgga gtgctgcccc ccaagtcgtg cccctgacca caagttgtgc tgtctggcaa 300

acattgtctg tgagaatgtt ccgctgtggc tgccaagcct ggtaacaggc tgccccagtg 360

tgcgtagttc tcatccagac ttcggtctgg caacttgctg ttaagacacg gcgtaagggg 420

cgtgtgccaa cgccctggaa cgagtgtcca ctctaatacc ccgaggaatg ctacgtaggt 480

acccctggtt cgccagggat ctgagcgtag gctaattgtc taagggtatt ttcatttccc 540

actctctctt tcttgttcat a 561

<210> 492

<211> 579

<212> DNA

<213> Murine kobuvirus 1 (isolate MKV1/NYC/2014/M014/0146)

<400> 492

gccaggtcgc atcgcctggt tggtgaccaa actgctagat gctcctccct ttccctcggg 60

gttggcgcac cgccatccct gcatcctccc cccagcctga catgctacag gctgtgcaag 120

aaccccgcga aagctgccac cgtggcaatt gtgggtcccc ctcttgtcaa ggcacagagt 180

ctttctccct taaggctaga cccctctgtg aattcagagg cggcaactag ttgtgccact 240

acatgcctca gacctcggtc gcggagtgct gttccccaaa ccctgacact gccgcgtgag 300

tatgctgctt ggcaagcatt gcctggatgg tcgttccgct ggggctgcca ggtctggtaa 360

cagaccgctc cagtgtgcgt agaccatctc cagcttcggc tggtgctcac aaggtaagac 420

tcggtgtaag gttcatgtgc caacaccctg gaatgagtga aaactctact gccctaggaa 480

tgctacgcag gtacccctgc tcccttggga gccagggatc tgagcgtagg ctaattgtct 540

gtcgggtagt ttcatttccc accctctaga ctgttcacc 579

<210> 493

<211> 180

<212> DNA

<213> Kobuvirus sewage Kathmandu (isolate KoV-SewKTM)

<400> 493

tccggcggag cgtcctcaga caaaactggc acagttgtct catcaggcaa ccgcgttggc 60

tcccgcttca ccaaatggtg ggagccagct gctgcgcgcg ccctcgagcg cgcgaccgat 120

gccaccatcg atggcatcga aggtgctggt aaggtcgcca ccaaggcgat ctccaaaaag 180

<210> 494

<211> 770

<212> DNA

<213> Bovine kobuvirus (strain IL35164)

<400> 494

cacccacttt tccggcggcg cacgttcgcg tcgtcgtaag tctggactcc caaggccaac 60

tcggttcaac ttcggtttcc ggacaaatac aagaacctca gtcctcttgg tactttctcg 120

cctgagtcaa caaagcgaga aacctgcccc tctaacgcca gacgagcggc atgaaacttg 180

aacttctggc atgttccact ttccctctcc ctcaccccac cccaccgcgc tctcaaggtc 240

gcgctcttcc gggactagcc cggttttaaa agttcctggc accctttgcc cctctaggcc 300

cttaaggtag gaactgacct tgtgctgtga tctcggtgcg gaagtgctac tgcgtagtga 360

ttgtaagatc cctttgtggt tctgccctgg caaggctaca gagtactgtg atccgctgcg 420

gacgccatcc tggtaacagg acccccagtg tgcgcaacag tatgtccacg gtcttccgtg 480

ttcaccacat tcggaacact gctctcgtga aacagtgtgt gtccaatccc tgtgaccagt 540

attaaccaca ccacctagga atgctaggaa ggtaccccgg ttcgccggga tctgatccta 600

ggctaattgt ctacagtggt gctcctttta ttttcccctc acttcattga ctacaactgc 660

ttgatttccg tgtttgctgc tcttcttagc tctcactgcc attctcaagt gttcacactg 720

tccaagctcc tttggttgtt tgcctccact tgccactgtc aactcttgtc 770

<210> 495

<211> 471

<212> DNA

<213> Kobuvirus cattle/Kagoshima-1-22-KoV/2014/JPN(Kagoshima-1-22-KoV/2014/JPN)

<400> 495

catccccaat atgttcggtg aggaatgcta ccacgtaact attagtgaac ccattgactg 60

ttctgccctg gcaaggctat agagtacagt gttccgctgt ggatgccgtc ctggtaacag 120

gatccccagt gtgtgtagct gtatgttcaa cttcggttgt tcacagtcct tggtctagct 180

tttagtgaaa tggtgtgcgc catatcccta catttgggtg ccacctctca acccctagga 240

atgctaggaa ggtaccccac cctccggtgg gatctgatcc taggctaatt gtctatgggg 300

ttgtttcctt ttcttttcac cactcattac tggctcacag attgtttgtt gtgttccctg 360

ctctctctag ctcccaacac ggcacacagt ctcagccaca ccgcctttgc aaacgactac 420

cagatttgtt tctttgactc ctctgattct cacatcttca ctgaaccaga c 471

<210> 496

<211> 665

<212> DNA

<213> Caprine kobuvirus (isolate MN1/2018)

<400> 496

gcccgtcccc ctcaccctct tttccggtgg ccacgcccgg gccaccgata cttcccttca 60

ctccctcggg actgttgggg aggaacacaa cagggctccc ctgtatttcc tcttcccatt 120

ccccctttcc taaccccaac cgccgtatct ggtggcggta agacacacgg gtctttccct 180

ctaaagcaca attgtgtgtg tgtcccaggt cctcctgcgt tcggtgcggg agtgctccca 240

cccaactgtt gtaagcctgt ccaacgtgtc gtcctggcaa gactatgacg tcgcatgttc 300

cgctgtggat gccgaccggg taaccggttc cccagtgtgt gtagtgcgat cttccaggtt 360

ctcctggttg gcgttgtcca gaaactgctt cgggtaagtg gggtgtgccc aatccctaca 420

agggttgatt ctttcaccac cttaggaatg ctccggaggt accccagcaa cagctgggat 480

ctgaccggag gctaattgtc tacgggtggt gtttccattt tctttttcac acaacttcat 540

tgctgacaac tcactgacta atcacttgct ctcttgtgcc tttctgctct ggttcaagtt 600

ccttgattgt ttgtttgatt gcttttcact gctttcttcc cacaatcctt gctcagttca 660

aagtc 665

<210> 497

<211> 473

<212> DNA

<213> Ferret kobuvirus (isolate MpKoV38)

<400> 497

ccatggtgta catcggtttc cgggcaaata caagaacccc agttttcctg gcaattcctt 60

gactgggtta aagaaataag gaaccagccc ctctaacgcc agacgagcgg ctttaaactt 120

gaacttctgg cacacttttc tccactaccc ttttccccaa actcggactg actcactgga 180

ttttgaccac tccccctttg tctgaccact cttttggatc tccctccaac atgccggtgg 240

tgcctggcgg aaaaagcaca agttgtgtga gcaacacccc cacaggtccc acggaacggt 300

ctgtgcccgc agatctagta cgtgcggatc cctggttgct tccactgctc gcctgtgagt 360

agagtaggtg ctgggtgtga tgcgtcttcg gacgcctcgg gcatagcatt gctcccccga 420

gaatcaagcc atcgagattt ctctggcctc tgaagtcacg cctggacgcg tag 473

<210> 498

<211> 641

<212> DNA

<213> Grey squirrel kobuvirus (isolate UK 2010)

<400> 498

ggatttaggc cgtggtgccc gtgggcacga cggccacggc tccagccgca ctcctctcca 60

cgccttgtcc cacgggacat aaagtggagc cctaagccct aaacccccat gttacccaat 120

tttcttatcc acttcccttt ttccctttga ctcacatttt tcttgaccgc gcttgtgagg 180

cggttcccgt acagttgagt tgacgggtcc cgcgcgggac ctgaaaatta ctccatactg 240

attaggaaag gttctagtac gcttgtaccc ttaaggccag cgggctacct cctttaggtc 300

ttatagcccc aactggttag aaccactcca gctcacctct atcggtgcgg gagtgctacc 360

acccctgcct gcgtcaagcc cgtttttcta gtgatctgtc cacaaacact tggattcccg 420

tcgcgactga cagaacctgg cgacaggtgt ctcgatgtgt gtgaccaagt ggcatgagaa 480

tcggaaacta acgctggtaa gatggtaagt gcccaatccc tgtggggtga tgtttttctc 540

tgcataggaa tgctgcgaag gtaccccggc cttgagtcgg gatctgatcg caggctaatt 600

gtcctctgtg cagttcattt ccttttcttt tcattggcat c 641

<210> 499

<211> 737

<212> DNA

<213> Marmot kobuvirus (strain HT9)

<400> 499

ggggcttcgg ccctcacacc ctcttttccg atggtctggt cccggaccac cgtcactcca 60

ttcagcttct tcggaacctg ttcggaggaa ttaaacgggc accccacttc ccccccccac 120

cctcgcaact tcaagtgtgt gtgcccataa ccttgacccc tgccggtatg ccgcccggtt 180

cagtggacaa actgctaggc aaattccccc ctccccctgt ggctggtcct tcaaccagcc 240

actctcttcc ccccagccgg acgtgctaca ggctgtgcaa agcccccgcg aaagctgctc 300

acgtggcaat tgtgggtccc ccctttgtca aggcttcgag tctttctccc ttaaggctag 360

tcccgtactg tgaaatcagt gtgggcaact agtgaagcca ctgcacgctc ccgaccacgg 420

acgcggagtg ctgtccccca agtcatgaca ctaacaccaa gctgtgctgc ctggcaagca 480

tagcctggga aggtgttccg ctgtggctgc caaacctggt aacaggtgcc ccagtgtgtg 540

taaccttcct ccagtcttcg gactggcagc tttgtgtgaa gacgtgacgt aaggttcaag 600

tgccaactcc ctggaaggga tgacccctct actgccctag gaatgctacg caggtacccc 660

tggttcgcca gggatctgag cgtaggctaa ttgtctacgg gtagtttcat ttcccacctt 720

tttatttctt tagcatc 737

<210> 500

<211> 792

<212> DNA

<213> Ovine kobuvirus (isolate SKoV-China/SWUN/AB18/2019)

<400> 500

gggtgggggg ggggggggat accctccccc ctcacatccc ttttcgacgg cgcgcctcgg 60

caaggccgcc gataccgact tgtgctacct cggtaccaca tttcgggaat aataatatgc 120

ccggtgtatg tttgcttgtt tctcgcaccg taccgacttc gggaatcaga aactagcacc 180

cccttccatc ccaccctttt cctctacccc tataatttac aacgctgtgc acagcagcgg 240

ccgccaagga ctagcctggc ggttataaaa ggaatctggg tctttccctc tctaagccat 300

aaggtagatt ccctgtgccc ctgaatgctc ggtgaggaat gctgcgtcgt aacgctttgt 360

gaagtgactc ttggttctgg cttggcaagc ctacagagta ccgtgttccg ctgcggatgc 420

catcctggta acaggacccc cagtgtgtgt agcggtatgt tcgggcttcg gtccgttcac 480

caagtttcat ggaccactgc gcgaaagcgc gtgcgccata tccctgtact tcaggtgtct 540

ttttctggga ccccaggaat gctgcgaagg taccccactt cggtgggatc tgatcgcagg 600

ctaattgtct acgggttcag tttccttttt ctttcctcct ccatcattga ctacctgatt 660

gattctagtt cctgtgcttt gcctgctccc tctgctctca acacggcttt ttcaaccagt 720

tctcgcactt cgaccaacac catttacacc ctcactttct ttgactcatc tgactcttgg 780

ctcactgaaa gc 792

<210> 501

<211> 668

<212> DNA

<213> Human parechovirus type 1 (PicoBank/HPeV1/a virus p123)

<400> 501

taccccgact tgctgagctt ctctaggaga gtccctttcc cagccttgag gcggctggtc 60

aataaaagcc tcaattgtaa caaacatcta agattattac attaacccca tgcctggtct 120

ccactagttg aaggcaactt gcaataaaat gagtggaaac aagacgctta aagtatggtg 180

taaattaaca tttctaactc acattttgtg tggggtggca gatggcgtgc cataattcta 240

ttagtgagat accatgcttg tggaccttat gctcacacag ccatcctcta gtaagtttgt 300

aagacgtctg gtgacgtgtg ggaacttatt ggaaacaaca ttttgctgta aagcatccta 360

ttgccagcgg aataacacct ggtaacaggt gcctctgggg ccaaaagcca aggtttaaca 420

aaccctttag gattggttct aaacctgaga tgttgtggaa gatacttagt acctaccgat 480

ctggtagtag tgcaaacact agttgtaagg cccacgaagg atgcccagaa ggtacccata 540

ggtaacaagt gacactatgg atctgatctg gggccaggta cctctatctt ggtgacctgg 600

ttaaaaaacg tctagtgggc caaacccggg ggggaacccc ggtttccttt tattttgtca 660

gtgccact 668

<210> 502

<211> 642

<212> DNA

<213> Human parechovirus 3 (strain CAU14/2015/KR)

<400> 502

ttctctagga gattcctttt cccagccaga ggtggctggt taaataatac ctattgtaac 60

aaaacatgta agacacaaca accgcacacc tggtctccac tggccgaagg caactagcaa 120

taaggcaggt gggttcagac gcttaaagtg tgttgtgcat attcttttct aacctgtgtt 180

ctacacaggg tggcagatgg cgtgccataa ccctaatagt gagatatcca cgcttgtgga 240

tcttatgctc acacagccat cctctagtaa gtttgtaaga catctgatga cgtgtgggaa 300

cttgttggag ataacatttt gctgcaaagc atcccattgc cagcggatcc acatctggta 360

acagatgcct ctggggccaa aagccaaggt ttaacagacc ctttgggatt ggttcaaacc 420

tgaattgtta tggaagacat ttagtacctg ctgatttggt ggtagtgcaa acactagttg 480

taaggcccac gaaggatgcc cagaaggtac ccgtaggtaa caagtgacac tatggatctg 540

atctggggcc aactacctct atctaggtga gttggttaaa aaacgtctag tgggccaaac 600

ccagggggga tccctggttt cctttttatt tgaataatca ct 642

<210> 503

<211> 708

<212> DNA

<213> Human parechovirus 4 (isolate K251176-02)

<400> 503

tttgaaaggg gtctcctaga gagcttggcc gtcgggcctt ataccccgac ttgctgagct 60

tctctaggag agtccctttc ccagccctga ggcggctggt taataatagc ctcaagcgta 120

actaatatct aagaagattg tgcaaaccct atgcctggtc tccactattc gaaggcaact 180

tgcaataaga agagtggaat caagacgctt aaagcataga gacagtattc ttttctaacc 240

cacattactg tggggtggca gatggcgtgc cataattcta atagtgagat accacgcttg 300

tggaccttat gctcacacag ccatcctcta gtaagtttgt gagacgtctg gtgacgtgtg 360

ggaacttatt ggaaacaaca ttttgctgca aagcatccta ctgccagcgg aacaacacct 420

ggtaacaggt gcctctgggg ccaaaagcca aggtttaata gaccctttag gattggttct 480

aaacctgaga tgttgtggaa aatatttagt acctgctgac ctggtagtaa tacaaacact 540

agttgtaagg cccacgaagg atgcccagaa ggtacccgta ggtaacaagt gacactatgg 600

atctgatctg gggccaacca cctctatctt ggtgggttgg ttaaaaaacg tctagtgggc 660

caaacccagg ggggatccct ggtttccttt tattttatca tatttaat 708

<210> 504

<211> 696

<212> DNA

<213> Human parechovirus 5 (strain CT86-6760)

<400> 504

tcctagagag cttggccgtc gggccttata cccgacttgc tgagtttctc taggagagtc 60

cctttcccag ccctgaggcg gctggtcaat aaaagcctca aatgtaacta acatctaaga 120

catttatgat tcaaccccat gcctggtccc cactagtcga aagcatctag ccataagatg 180

agtggactaa ggacgcttaa agcatggagc caacaaccat ttctaaccca tgttttgcat 240

ggggtggcag atggcgtgcc ataactctac tagtgggata ccacgcttgt ggaccttatg 300

cccacacagc catcctctag taagtttgta agacgtctga tgacgtgtgg gaaaacacta 360

gaaatattaa cttgctgtaa agcatcctac tgccagcgga atcacatctg gtaacagatg 420

cctctggggc caaaagccaa ggtttagcag accctttagg attggttcaa tacctgagtt 480

aatatggaaa gtgcttagta cctgctgact tggtagtagt gcaaacacta gttgtatggc 540

ccgcgaagga tgcccagaag gtacccgtag gtaacaagtg acactatgga tctgatctgg 600

ggctggatac ctctatcttg gtgatccagt taaaaaacgt ctagcgggcc aagcccaggg 660

gggatccctg gtttccctat ttttattatt atcact 696

<210> 505

<211> 618

<212> DNA

<213> Human parechovirus 5 (4112/SapporoC/July/2018)

<400> 505

ggcggctggt caataaaagc ctcacatata actaacatct aagacaataa tgtacaacct 60

tgtgcctggt ccccaccggt cgaaggcatc tagcagtaag ataggtggaa caaggacgct 120

taaagcacaa agacaaccta tttttctaac ccgcaaatta ttgtggggtg gtagatggcg 180

taccataatt ctactagtga gataccatgc ttgtggatct tatgctcaca cagccatcct 240

ctagtaagtt tgcaaggcgt ctggtgacgt gtgggaactt attggaaata acatcttgct 300

gcaaggcatc cattagccag cggaataaca cctggtaaca ggtgcctctg gggccaaaag 360

ccaaggttta gcagacccta atggattggt tcaaacctgg gatgttgtgg aagatactta 420

gtacctaccg atctggtagc aatgcaaaca ctagttgtaa ggcccacgaa ggatgcccag 480

aaggtacccg taggtaacaa gagacactat ggatctgatt tggggccaac tacctctatc 540

ctggtgagtt ggttaaaaaa cgtctagtgg gccaaaccca ggggggatcc ctggtttctt 600

ttacttatca ctgtcatt 618

<210> 506

<211> 709

<212> DNA

<213> Human parechovirus 6 (strain: NII561-2000)

<400> 506

tttgaaaggg gtctcctaga aagcttggcc gtcgggcctt ataccccgac ttgccgagtt 60

tctctaggag agtccctttc ccagccctga ggcggctggt caataaaagc ctcaaacgta 120

actaacatct aagattgatt taataacacc atgcctgatc cccaccagtc gaagacaact 180

agtaataaga cgggtggaat aaggatgctt aaagcatggc gtaaatattc ttttctaacc 240

catgatttca tggggtggca gatggcgtgc cataactcta ttagtgagat accacgcttg 300

tggaacttat gctcacacag ccatcctcta gtaagtttgt gagatgtcta atgacgtgtg 360

ggaacttatt ggaagcaaca atttgccgta cggcatccta ctgccagcgg attcacacct 420

ggtaacaggt gcccctgggg ccaaaagcca aggtttaaca gaccctttag gattggttaa 480

aacctgaatt gttgtggaag atactcagta cctgctgatt tggtagtaat gcaaacacta 540

gttgtaaggc ccacgaagga tgcccagaag gtacccgtag gtaacaagtg acactatgga 600

tctgatctgg ggccaactac ttctatcatg gtgagttggt taaaaaacgt ctagtgggcc 660

agacccaggg gggatccctg gtttcctttt ttatttcact acagccaat 709

<210> 507

<211> 616

<212> DNA

<213> Human parechovirus 6 (isolate AFW)

<400> 507

cggctggtca ataaaagcct caaacgtaac taacatctaa gattgattca ataaccccat 60

gcctgatccc caccagtcga agacaactag taataagacg ggtggaacaa ggatgcttaa 120

agcatggcgt aaatattctt ttctaaccca tgattctatg gggtggcaga tggcgtgcca 180

taactctatt agtgagatac catgcttgtg gaacttatgc tcacacagcc atcctctagt 240

aagtttgtga gatgtctaat gacgtgtggg aacttattgg aagcaacaat ttgccgtacg 300

gcatcctact gccagcggat ccacacctgg taacaggtgc ccctggggcc aaaagccaag 360

gtttaataga ccctttagga ttggttaaaa cctgaattgt tgtggaagat ccttagtacc 420

tgctgatttg gtagtaatgc aaacactagt tgtaaggccc acgaaggatg cccagaaggt 480

acccgtaggt aacaagtgac actatggatc tgatctgggg ccaactactt ctatcatggt 540

gagttggtta aaaaacgtct agtgggccaa acccaggggg gatccctggt ttccttttta 600

ttttactaca gccaat 616

<210> 508

<211> 511

<212> DNA

<213> Human parechovirus 7

<400> 508

ggttcagacg cttaaagtgt gttgtacata ttcttttcta acctatgttt cacatagggt 60

ggcagatggc gtgccataac tctaatagtg agataccacg cttgtggacc ttatgctcac 120

acagccatcc tctggtaagt ttgtaagacg tctgatgacg tgtgggaact tgttggaaat 180

aacagtttgc tgcaaagcat cccattgcca gcggattaac atctggtaac agatgcctct 240

ggggccaaaa gccaaggttt aacagaccct ttgggattgg ttcaaacctg aattgttatg 300

gaagacactt agtacctgct aacttggtag taatgcaaac actagttgta aggcccacga 360

aggatgccca gaaggtaccc gtaggtaaca agagacacta tggatctgat ctggggccag 420

ctacctctat cttggtgagt tggttaaaaa acgtctagtg ggccaaaccc aggggggatc 480

cctggtttcc ttttatttta aaatattcac t 511

<210> 509

<211> 637

<212> DNA

<213> Human parechovirus 14 (clone V3C)

<400> 509

tccctttccc agccttgagg cggctggtca ataaatgcct caaatgtaac taacatctaa 60

gattaactta gacaacccta tgcctggtct ccgctagtcg aaggcgcctt gcaataagac 120

gagtggaaca aagacgctta aagcatagtg aaaatataca tttctaacct gtggcctcca 180

cagggtggca gatggcgtgc cataactcta ttagtgagat accacacttg tggaccttat 240

gctcacacag ccatcctcta gtaagtttgt aagacgtctg atgacgtgtg ggaagttatt 300

ggaagtaaca atttgccgta aggcatccta gagccagtgg attaacacct ggtaacaggt 360

gcccctgggg ccaaaagcca aggtttaaca gaccctttag gattggttca aacctgggtt 420

gttatggaag atatttagta cctgctgatt tggtagtaat acaaacacta gttgtaaggc 480

ccacgaagga tgcccagaag gtacccgtag gtaacaagtg acactatgga tctgatttgg 540

ggttgactac ctctgtcctg gtgagtcaat taaaaaacgt ctagtgggcc aaacccaggg 600

gggatccctg gtttcctttt attttattac aaccatt 637

<210> 510

<211> 667

<212> DNA

<213> Human parechovirus 17 (isolate 157Chzj058)

<400> 510

cttgtttcca ctcattttat tgcaagttgc cttcaactag tggagaccag gcatgaggtt 60

aatgctataa tcttagatgt ttgttacagt tgaggttttt attaacctca tgcctggtct 120

ccactagttg aaggcaactt gcaataaaat gagtggaaac aagacgctta aagcatggtg 180

taaattagct tttctaaccc acactttgtg tggggtggca gatggcgtgc cataactcta 240

ttagtgagat accacgcttg tggaacttat gctcacgcag ccatcctcta gtaagtctgt 300

gagatgtctg gtgacgtgtg ggaacttatt ggaaacaaca ttttgctgta aagcatccta 360

ttgccagcgg aacaacacct ggtaacaggt gcctctgggg ccaaaagcca aggtttaaca 420

gaccctttag gattggttca taacctgaaa tgttgtggaa gatacttagt acctaccgat 480

ctggtagtat tgcagacact agttgtaagg cccacgaagg atgcccagaa ggtacccgta 540

ggtaacaaga gacactatcg atctgatctg gggccaggta cctctatctt ggtggcctgt 600

taaaaaaacg tttggggggc cagcccgggg ggggtccccg ggtttctttt atcttacaac 660

tgctata 667

<210> 511

<211> 592

<212> DNA

<213> Human parechovirus 18 (isolate 11Chzj207)

<400> 511

cgtaacaaac atctaagatt atcatattaa ccccatgcct ggtctccatt agttgaaggc 60

aacttgcaat aaaataagtg gaaataagac gcttaaagca tggtgtaagt tttcttttct 120

aactcacatc ttgtgtgggg tggcagatgg cgtgccataa ctctattagt gagataccac 180

acttgtggac cttatgctca cacagccatc ctctagtaag cttgtaagac gtctggtgac 240

gtgtgggaac ttattggaaa catcattttg ctgcaaagca tcctattgcc agcggaacaa 300

cacctggtaa caggtgcccc tggggccaaa agccaaggtt taacagaccc tttaggattg 360

gttctaaacc tgaaatgttg tggaagatat ttagtaccta ccaatctggt agtattgcaa 420

gcactagttg tacggcccac gaaggatgcc cagaaggtac ccgtaggtaa caagtgacac 480

tatggatctg atctggggtc aactacctct atctaggtga gttggttaaa aaacgtctag 540

tgggccaaac ccagggggga tccctggttt ccttttatct taaatagtga ct 592

<210> 512

<211> 528

<212> DNA

<213> Human parechovirus 19 (isolate 67Chzj11)

<400> 512

ttgtgtcaaa tttgtagtgt ttacagtagt tgtagcttgc acgacatcct tgttctccgg 60

ttgggctgtg gttgcaaaac tatttggtcc tagtaaatta gaagcatcat ctgcaacttt 120

ggttattatt tccccgccaa cttcattgcc agaaataata ttattagctt cagtttctac 180

attagacaag gagagagtat ccatagcacc agtagcaaca ttaactaaat ccttgataga 240

ctccataatg gctgtttaaa attaaaagaa aaccagggat cccccttggg attggtttaa 300

acctgaacta ctatggaaga catttagtac ctgccaattt ggtagtagtg caaacactag 360

ttgtaaggcc cacgaaggat gcccagaagg tacccgtagg taacaagaga cactatggat 420

ctgatctggg gccaactacc tctatcttgg tgagttggtt agaaaacgtc tagtgggcca 480

aacccggggg ggatccccgg tttcctttat ttaataatta gattcatt 528

<210> 513

<211> 759

<212> DNA

<213> Ljungan virus strain 145SL (isolate 145SLG)

<400> 513

ttgaaagggg ggccctgcag ccgatatagg ctgcagggtt cccctggtgg ggtgggtacg 60

tctctcgctc attgagtggg ggcgtggctc accaaccaca cctggttggt ccccaggttc 120

atgcaataac cactttttgt aatctttaca tctaagctta attcacccac tagaactgga 180

cgaaaccgct tggaataagt ttggttctct cttgagtgtg ttttgtgtta gcataatttc 240

tgtctctaga gtgctttaca ctctagtagg ggctgtaccc gggcggtccc actcttcaca 300

ggaatctgca caggtggctt tcacctctgg acagtgcatt ccatacccgc tccacaatag 360

aagatgatgt atatctttgt ttgtgaaatg ctcatgaaac gtgtgtgtag gcgtagcggc 420

tacttgaatg ccagcggaac ccccctagtg gtaacactag cctctgggcc caaaaggcat 480

gtctctgacc attcaagtac acaaccccag tgatacacac atttagtaat ggctcagtaa 540

tggacattga ttgatcatca gacaattgtt aggaggccta ggtatgacgg gctgaaggat 600

gccctggagg tacccgcagg taaccttaag agactgtgga tctgaccagg ggcccaccat 660

ggaaacatgg gtagaagtct tcggaccttg ggttaaaaaa cgtctaggcc cgccccccac 720

agggatgtgg ggtttccctt ataaccccaa tatcacatt 759

<210> 514

<211> 634

<212> DNA

<213> Ljungan virus M1146

<400> 514

agaatagcca atctgtaact tcaacaccta agcgcatctt gcccgccgtt atctggtcga 60

aggcacttgc aataagactg gttaaccctc catgcgtgat tcacaatcct atctgtctct 120

cgagtgcttt acgctcgggt aggggctcac ccaggaggtc ccactcttta cagaggtctg 180

cgcaggtggc tttcacctct cgacagcgcc cctcaatcct gccttaggtg tgaaggttgc 240

acgtattcca atgtgaacag tgcttgaatt ttgttggtag gtgtagcgac cacacatgag 300

ccagcggatt tcccctggtg gtaacaccag cctctgggcc caaaaggcat gtctttgacc 360

tcatgtgtgc acaatcccag tgatgccaat gattagtaaa gcacttagta tatatatggg 420

atataccaat ctcaagcacc taagggtgta ggtatggtag gctgaaggat gccctggagg 480

tacccgcagg taacgattgg ttcactgtgg atctgaccag gggcccaccg gaacttcgtt 540

ctgggtagaa gctctcgagc cttgggttaa aaaacgtcta ggcctatccc ccggggggac 600

ccggggtttc ccttataacc ccatattttg catc 634

<210> 515

<211> 584

<212> DNA

<213> Ljungan virus 64-7855

<400> 515

ggtcgaagcc gcttggaata agactggtgc tgttcacagt gtgttcacga tctatgcaac 60

ttctgtcttc catctgtgtc tatgcaggtg gataagggct gtacccgggc ggtcctactc 120

tccacagggg cctgcgtagg tggctttcac ctctggacag cgcaccccac cccgcctact 180

gagtgaaggg catacggctt ttctattgcg aattgtattg aagtttagga ttaggtgtag 240

cgaccacatg tgtgccagcg gatctcccct ggtggtaaca ccagcctctg ggcccaaaag 300

gcatgtctct gaccacacat gtgcacaacc ccagtgataa tcctttttag taaatgctta 360

gtaacagtgg attagcagtg ctcctcaagc tcagtagggc ttaggtatgg caggttgaag 420

gatgccctgg aggtacccgc aggtaacgat aagttcactg tggatctgac caggggccca 480

ccgggaattc cccgggtaga agccttcggg ccttgggtta aaaaacgtct agacctgtcc 540

cccagaggga tctggggttt cccttttaac cccaattatt caat 584

<210> 516

<211> 740

<212> DNA

<213> Rattus tanezumi parechovirus (strain Wencheng-Rt386-3)

<400> 516

cccgtggctt atgccacggg acccgtgccc ccgtccaggg ggttttactt atgctcaggc 60

gtttgcatta tcgcctggat ctggctctgg gcaccctttc agcccctggg catgggttta 120

aactgcctac ctgtaacact taacatctaa gaaactgatt tgtaaccaac cctggccaga 180

tgttgccagc ccaagccact tggaataagg tgggcaatgt cacattggtg cttaaaggtt 240

gcgtccaaat ttttcttttc caaccactca tgtggacggt gtgcaaactc caccgtaaca 300

cttctaggtg acttccacac ttgtggggct aaagcaccag agtctttgct tcaaccatat 360

tgtggtgaca gcaaagcatc ctgaaattag ttagtaatga ttctagatcc acttctgtgg 420

tgctgcatgc ctgcggagca atggggggtg accctcacct caggggccga aagccacggt 480

gtttaaagca cccatgcagt atgcttgtct gtttaggatt gtgaacactg aaagtaagga 540

tgatgagtgg tcactgtggt cactggttgt cgtacggggg ctgaaggatg cccagaaggt 600

acccgtaggt aaccttaaga gactatggat ctgatctggg gccagggagt gatgtactct 660

atacacctgc cttggttaaa aaacgtctag gcccccccaa cctgggggac caggttttcc 720

tggtcatttt gcaaaggaat 740

<210> 517

<211> 696

<212> DNA

<213> Parechovirus (sp. strain Parchzj-6)

<400> 517

gtggagacca ggcatggggt taatgtcata atcttagatg tttgttacgt ttgaggcttt 60

tattgaccag ccgcctcagg gctggtcaat aaaagcctca aacgtaacaa acatctaaga 120

ttatgacatt aaccccatgc ctggtctcca ctagttgaag gcagcttgca ataaaatgag 180

tggggttaag acgcttaaag catggtgtga attaactttt ctaacccaca ctttgtgtgg 240

ggtggcagat ggcgtgccat aattctatta gtgagatacc acgcttgtgg accttatgct 300

cacacagcca tcctctagta agtttgtaag atgtctggtg acgtgtggga acttattgga 360

aacaacattt tgctgaaaag catcctatcg ccagcggaac aacacctggt aacaggtgcc 420

cctggggcca aaagccaagg tttaacagac cctttaggat tggttcaaaa cctggaatgt 480

tgtcgaagat acttagtacc tgccaatctg gtaatagtgc aaacactagt tgtaaggccc 540

acgaaggatg cccagaaggt acccgtaggt aacaagtgac actatggatc tgatctgggg 600

ccaggtgcct ctatcttggt gacctggtta aaaaacgtct agtgggccaa acccgggggg 660

gatccccggt ttccctttta ttttatcaat gctact 696

<210> 518

<211> 495

<212> DNA

<213> Baskerville virus

<400> 518

cctaagctta cattcacact tttaggtcac ttccgatcga aaccgaatca ctaaaagcta 60

caagaatgcg gacgtctaca tgctataaac cagactacaa actactaaaa gattaataaa 120

atataaaatc tagaacatat cactaattaa aattaatgcg cgttatgcgt gtgcgctccc 180

acgtgatgaa atagatcatc ataaaggcac gttgttcgtg tgtgctccca cgtaacacgt 240

tatatcgtgt gcgtctccac ggatagttta gagttgatat cagcaacaaa gtagatgatt 300

agaaatcaaa tctcaaaaag agaaaccctt tcttcgattt cacctactcg gagtagcaca 360

ttggtattcc aacgtatata tagatcacga gttgtatatc tacatacagt cacaaatgcc 420

aggacaaaca gtacaattaa taaaactgaa actccggcaa actgtgcaac tactcctgca 480

caaggaccaa acata 495

<210> 519

<211> 836

<212> DNA

<213> Bemisia tabaci picorna-like virus 1 (isolate CAU-Q1)

<400> 519

tcttaaaagt agcttagtaa gtggcgcacg gggataatac cccgtgcgtc acttactaag 60

ctacttttaa gatctctttt gagaaccata tttattggct aataggttac catttttagt 120

caatattatg gcttaccaag agagatgtgt ttttcatagc ttaaatttat aaacattatg 180

tttttatttt aaatatttag tatattagat cgattatgta tttgacattt caatgtagta 240

tattttggac ttttaatgat ggacggaaaa gcaccgtgtt taaaaccttc gattggagaa 300

atcctaaaga catgcaatgt tcctataccc acaaaaagat ttattataaa atgggtttta 360

tattgctagt gcctcgaagc taatttacac aaggcaagta cctgatgcaa atgatttatt 420

agtgcatata tatatgaata gttaatctta gataggacgg atgattgaca ctcatcctta 480

ccttcctttg atttgacaga tacataatat atcttttgaa ttgaggttaa atatgtaatt 540

agggatgtaa tggtagaagt gatccttttc ctcaattata gtggtgaact actaaataat 600

gttaattttt aaaactaatg gcgagattga gatgtttgat taattaacat ttgatgtggt 660

actgccggcg gattagcatt gccgtacgga gggagctcac ttaagaaacc gaagcgttaa 720

caacgtgagg taacttctac aaattttatt ttattttaat atatgtttgt cattttgtgt 780

tttgcgttct ttgcgtttta ttgtgtttat tctgcgtttt tatagtttga tttaat 836

<210> 520

<211> 849

<212> DNA

<213> British Admiral virus (isolate MW13_1o)

<400> 520

acggagttta cgtttctttt acccatgcgt tcgcgtatgg ggttttcttg caaccactat 60

ggttcgccat agttggatgc gatccccgtc ctcactaacc gacgttaaac tgttggtatt 120

gtaacctcca atccccccta acctaagtct atccagtttc ttggtatctg caagaaccaa 180

gcgcacacca caagtggcga caagacttgt ggaggatcat gcactaaagc atgcgttcta 240

tgcacgtttt atctagagac gcgtatatga atgttatctg ttatctagac cccttattcc 300

ccttgtgcca tggtgccact ggcctatgag tcgggtcctc tcctgacccg tcagcaccca 360

ttctaaagac tgttagcaca gttattagtc tgtgaactct cgcgaaagcg ttagtgtttt 420

ctagcgatgc attgattgcg cgtggaaccc ccacctggtg acaggtgccc cgcggcccaa 480

aaggctagtg tgtcacaaca caccgtcaat gtgccagaat ccccatctgg cagcactaat 540

tgttagtacc gattagtaac cactaatcat ctgtcctggc cgaacactct ttggaatgag 600

cataggagtt agtagacccc ctagcgctac cctcgggcgt gactagggcc agttgactat 660

actgttccgg taataactgg ttaaaacaat gaaagctcac tatgcgaact gccctaccca 720

agggggccgt cctcacggcc ttccccgaac gtcggcagtc tttccttctc tttttatcaa 780

aatgttttat ggtgacaata ttgtgtattt agtcttgttg ctttctttgg ttttatctaa 840

taattttat 849

<210> 521

<211> 975

<212> DNA

<213> Carfax virus

<400> 521

cacggggtta acacctcgtg cgatcttttc aaccacatat atatatattc tgggcggaat 60

attagaaaat cttgggatta tatatatgta attgatatat tgggcggaat atttgaaaat 120

cttgatatat ctcttacaaa attttgaaac caaccatttt agaataggtt tattagcgtg 180

acaaactatg cgacctgtag agaaaaatgt caaaatcaat acaaaatttt aaaatgttaa 240

aaatgtttat atatttagta aactcaagtt gaattgtgat agagaaggca agtaactacc 300

agatgactct tctcgcaaca ggattatccc agactgttgt tccagcaaat tgatggatgt 360

aaactaaatt tataatgata aatagttaag taggagaccc ttatggtact caagcttgta 420

ttaaaatttt taactataga tatgtattat tgaaatgtgt tctgtctctt atatgtacac 480

ccacgtagtc gtggtaccgc tataacgcca atcggactaa ataacaagaa tgtgaggttc 540

ctattgagat ttcaaaattt atcatattgt cacgaccaat ctctttgggt aaggacttgc 600

atggtatgcg aaaggtcgga ttgccagaat ctgcttgctg ctttctggat gtgtacacat 660

tttaatttta agatttatga tagttaattt gataatacaa ggacagtgcg ttttgtgacg 720

tgcttgtttg catctctgtc ttagatgaca ggtttctaca ctaggcgatg tgtagaagtg 780

tagacctcta atggtttggt gcatcgtgcc ccggttcttt ttcaaggctt ctccggttca 840

aagatagcgg aggacccccc tttgtaatgc agacaccatt gtggataatt tatctacaat 900

tcgaagcgat aatttttcca ttcatttttg tatctttaaa acttcttacg gagattttca 960

atattaacta gaaaa 975

<210> 522

<211> 663

<212> DNA

<213> Chicken picornavirus 4 (isolate 5C)

<400> 522

gggggctctc ccccacggcc tcttttcccg accggtctgg caaaccggac ggttatcctg 60

gttaggtgtc cgatggatgc tggaacgggg tggctatagc tgcctccttc tggcttcctt 120

tattgggcat tctagctggg ccctttgcca aaattccatc ttaatcttaa acccattttc 180

tatacccagg cttgcttgta actggtaagt tgacccactg gcttgtctta agactattct 240

ggtgttggtg gacactcctt ccacaagtaa gtattgtgga gttcatgctc gttctgaacc 300

gggaatggca caccccgtag taggatcttg cctctgccac atatacttgc gcctgttgct 360

tctagactat gggctactaa ggatgactac ggaccccttt ttgggtaatc tgtgtcaagt 420

ctactgtatc atctggtgta cgctggaaat ccttgttctg gaaactgcgt ctggaggtgg 480

tgctgaaata ttgcaagcca cagatgcatt tctggtcttg gtgccgctac taggtgttac 540

accccctgtg ggcaaatgta gaaccttggt ggacgcgtga gatgggaatc cacgcctggc 600

catagactgg ctgataagct cgcggctgat cgagttgcaa cagtaatcag ttgatttgcc 660

act 663

<210> 523

<211> 679

<212> DNA

<213> Chicken picornavirus 5 (isolate 27C)

<400> 523

aaaaggggtc cgtgggggtt ctcccccacg gctccttttc ccgaccggtc tggcgaaccg 60

gacggttatc ctggttagat gtctgatggt tgctggaacg tggtggctat agctgccacc 120

ttctggcttc ctctattggg cattctagct gggttctttg ccaaaattcc atcttactct 180

ttacccattt tctctaccca ggcttgcttg taactggtaa gttgacccac tggctttagt 240

tcatgattat tctggtgttg gtggacactc tttccacaag taggtagtgt ggagttcatg 300

ctcgttctga accgggaatg gcacaacccg tagtaggatc ttgcctctgc cacacttact 360

tgcgcctgtt gcttctagac tatgggctgc taaggatgac tacggacctc tttttgagca 420

atccgtgtca agtcaactgt ttcatctgat gtacgctgga aatccttgtt tctggatgat 480

tgtatcctgg aggtggtgct gaaatatctc aagccacagg tacgcttctg gacttggtgc 540

cgctgtcagg tgttacaccc cttgtgggca aatgttgaac cttagtggac gcgtgagatg 600

ggaatccatg cctggccata gactggctga taagctcgcg gctgatcgag ttgcaacagt 660

aatcagttga tttgccact 679

<210> 524

<211> 447

<212> DNA

<213> Chicken proventriculitis virus (isolate CPV/Korea/03)

<400> 524

tggctttgtt cttagactat ttttggtgtt ggtggacact ctttcsacaa gtaggttata 60

tggagttcat gctcgttctg aaccgggaat ggcacaaccc gtagtaggat cttgcctctg 120

ccatattaat ctgcgcctgt tgcttctaga ctatgggctg ctaaggatga tgttggaact 180

cctttttgga tattccatat caagtcaact gtttcacctg gtgtacgctg gaaatccatg 240

ctttaagtcc tcatccggag gtggtgctga aatattgcaa gccacggatg actcttgaag 300

cctggtgccg ctactaggtg ttacaccctt tatgggcaaa tgttgaacct tagtggacgc 360

gtgagatggg aatccacgcc tggccataga ctggctgata agctcgcggc tgatcgagtt 420

gcaacagtaa tcagttgatt tgccact 447

<210> 525

<211> 619

<212> DNA

<213> Zebrafish picornavirus-1 (strain NCSZCF/ZfPV/2015/North Carolina/USA)

<400> 525

tgaacgcatg cgcatatcct ctttcccccg gagcctcacc ggggaccgcc tcatggtgac 60

ctgatcggtc actactgtgg tggtgaacaa atgtctagga cctttcactc caaatccctt 120

tccccttttt ctacttctta gtacgcctcg gctcgctcat ccgttgtgtg ctcaaactcc 180

cctcctccta caccctttcc ccccccctcc cgagtgactc gcacttagtc gtaggtttgg 240

tgtctttcac catttctgtg cctatctatg tgtcctttgg atccgccctc gaatgccctc 300

tagggtcttc tgcgaaagct tgcaaagccg cgccccccct ccactgttct tggggaccgt 360

gcgcagccgg aacttttgac atttgactga acagtacagc aatgtagcac gtctcttgtc 420

tttgccggtt agccctcgtg gtcaaacttt gtaagttagt acacttaagc actctcctgt 480

ggtttctctt gggaccttcc aattgtagga atccttggta tgccttcgaa tagggacgga 540

cgctctaaat gccccgttcg cgaagtcgac gcctgaatga ccaaggtttt tccacctttc 600

ctcaaaaccc ttcttttta 619

<210> 526

<211> 836

<212> DNA

<213> Duck picornavirus (duck/FC22/China/2017)

<400> 526

tcttaaaagt agcttagtaa gtggcgcacg gggataatac cccgtgcgtc acttactaag 60

ctacttttaa gatctctttt gagaaccata tttattggct aataggttac catttttagt 120

caatattatg gcttaccaag agagatgtgt ttttcatagc ttaaatttat aaacattatg 180

tttttatttt aaatatttag tatattagat cgattatgta tttgacattt caatgtagta 240

tattttggac ttttaatgat ggacggaaaa gcaccgtgtt taaaaccttc gattggagaa 300

atcctaaaga catgcaatgt tcctataccc acaaaaagat ttattataaa atgggtttta 360

tattgctagt gcctcgaagc taatttacac aaggcaagta cctgatgcaa atgatttatt 420

agtgcatata tatatgaata gttaatctta gataggacgg atgattgaca ctcatcctta 480

ccttcctttg atttgacaga tacataatat atcttttgaa ttgaggttaa atatgtaatt 540

agggatgtaa tggtagaagt gatccttttc ctcaattata gtggtgaact actaaataat 600

gttaattttt aaaactaatg gcgagattga gatgtttgat taattaacat ttgatgtggt 660

actgccggcg gattagcatt gccgtacgga gggagctcac ttaagaaacc gaagcgttaa 720

caacgtgagg taacttctac aaattttatt ttattttaat atatgtttgt cattttgtgt 780

tttgcgttct ttgcgtttta ttgtgtttat tctgcgtttt tatagtttga tttaat 836

<210> 527

<211> 625

<212> DNA

<213> Eotetranychus kankitus picorna-like virus (strain EKPLV.abc9)

<400> 527

cctattgaga agtgaacttt cattttctat acaaagtgct atatttcgag cgacagtgta 60

aaactaaaca atagtgtaca taacttatta ctcaaaatat agttaagaca aaagtcctct 120

ggaccccagt ccagaggatc tttgtcttaa ctatattttg agtaataagt tatgtacact 180

attgtttagt tttacactgt cgctcgaaat atagcacttt gtatagaaaa tgaaagttca 240

cttctcaata ggagtatgta cttagtagat tatataacag ctgattatta ttggctgaaa 300

taatccgtaa taatcgtaag tcgattatat aagactacac gctcattttg tggttatttc 360

aaatttaaca acttttgaaa ccactttcat tttaagatgt gtgttctatt tatctagacg 420

catattcata ttcacttgtt tagtaatttc accctactaa actcgtattc acgatacgta 480

ttttccagaa ttagtttact aatgagatta tacgcattaa cattttcgtt agacttttga 540

tacatacaaa tctaaccctt gtttggtagg caagtacaac ctactggttt cccgagacct 600

atgaggggcc caataaatat gaaag 625

<210> 528

<211> 867

<212> DNA

<213> Falcon picornavirus

<400> 528

tttgcgcgcg ggacctctgc tacggccggg ctcatattat cctccggggt tttatgggtc 60

gggcctagcc cctttctgta acggtctgtg tgagacctaa aaccacacat aatatcgtgc 120

ctgatggtta acgcttgtta gccgtctgta aactaagaac ctaccaacac actatgcacc 180

tcttggcgaa gccgcttgga ataagttgag aggaattatg catgctagtt gtgtttgttt 240

acaactaatt gttctaatcc aagtgaagct cttcgcttgg ggcggcacga cacttgccgt 300

aattcttcta ccgtccctcc acaccttgtg gatgaagggc cggatgtgtg gcctctggct 360

aacccctctc tctggggtga tgctactgga tgttttactc ctagaccaaa tcacatgaac 420

tcctcttgat ccacttcggt ggggctatga gcctgcggat taatagctgg cgacagctac 480

cccaggggcc aaaagccacg gtgttagcag caccctcata gtctgatgcc caagggctga 540

tgttgggagc tagtagtgtg tgtctggcct atgtttagga cttctggcca agcgcagagg 600

agtggggctg aaggatgccc agaaggtacc cgtaggtaac cttaagagac tatggatctg 660

atctggggcc ccctcacgtg gctttaccac gtgttggggg ttaaaaaacg tctaggcccc 720

accagcccac gggagtgggc tttcccttaa aaagcccaac aatatttatg gtgacaattc 780

actgtttctt ctttgcaatt tttgtattct tggactcctt attttttgtt tgcttgattt 840

tagtggacat tcagattcaa atacaaa 867

<210> 529

<211> 303

<212> DNA

<213> Feline picornavirus (strain 661F)

<400> 529

tggccaccgg ggaaaaacgg ctactgtgca ccacctctgc accccacgcc gaccacgcct 60

aaccatggcg ttagtaggag tggaggacca ctgcagaggg tcctggtgtg cgacagtcag 120

tggtagagta gacagtcctg actgggcaat gggaccgcgt tgcgtatccc taggtggcat 180

tgagatttct ctgccaccca ccagcatgga ttcctgtggg gggggcccca tgggctaggt 240

ctatactgcc tgatagggtc gcggctggtc gactgctgac tgtataacca gttgtaactt 300

act 303

<210> 530

<211> 542

<212> DNA

<213> French Guiana picornavirus (isolate French_Guiana_Picornavirus)

<400> 530

tgttcagcca cgatggcaag tggtaaaatc ttgcccgcat tctttaagtg cgttacttta 60

tcttctcctt tatatcctat cttttccaag gtgtagctca catctttagg cgggtcttat 120

ttataccgtc accgagcggg tggaagtgaa gactatagtt agtaaactca ctttagtaag 180

tccttttggt acgtaactgt caggggcttg gactgggggc cgtaggtggg gagacccaca 240

cccccggtgg ccaaaagcct agtggatcgc aatccaccgg ccctggccac tcttttgagt 300

ggatccttaa ggaaagaacg ggtagcgaaa cctatagggg atccgaaacc gtcaggggga 360

gccgagtaga tgccctggga taactggagc ggccgatgtg acgcccggtg tcttttcatc 420

gggcgcacgc gcccgcccca ggtaaccagg ggccaggcta cttacaagct ggttaaaaac 480

catgaaagga atacccccga aattgctaac taacttatct ctgtggtgtt gtggtgggca 540

cc 542

<210> 531

<211> 587

<212> DNA

<213> Leveillula taurica associated picorna-like virus 1 (isolate PM-A_DN31116)

<400> 531

caatagggta acctattgta acttggactt attactttat catatagttt attgtttaaa 60

tcggaactag tgtattatgt ttaaataata tgtgttagcc tttaaacttt attctattat 120

ttgataaggt tcaataagtt tgtatgacta gatccagtaa ctattgctca attttaaaac 180

aatcttaaaa atctgtatga aacgcagatc aatttaatat tttagaacta cccgtattta 240

tataggctct tctaaaatat gaattgttat cgaaagatac cttattattg gttgattaat 300

ttcttaatgt gcatggtggc acctttaata agtagatttc cgtcattctc tatttagagt 360

acttttggac gttaactaat gctccattca gtctagaatg tatgataatt aaactctaga 420

ttgtataaac tatcattatc gcaagtacca catcaaataa aacttatgaa aaaccgcaat 480

tactcgatga taactttgct cctgatgtta gtgatattac taatactaac tttctcgagg 540

aaagggtagt cgaaactagg gaagcatatc catcaacatc tcttact 587

<210> 532

<211> 621

<212> DNA

<213> Moran virus

<400> 532

gggggctctt caagcccccc caaccgaatt ttcttcaaca actactatgt aagttgcatt 60

atactctcca gatcaattcg aatttgtata atgtacttta gtttttgttg tcgactacag 120

tgattataag taagtagcaa tatgtgtcca gatcaattcg aatcacaata ttgttcatta 180

taattgtatg taggtctcag ttgagagaaa aattttgttt tgaaaaccca gttttttcac 240

accggaggaa tgaatctcaa atcttgggtt ttcgaggttg tcgtgcggac acttctcgag 300

tagttgaatg atgctgattt gactgctttg gaatgttgtc ttttgcgatg atgtcggatt 360

cacacgggct tcacatgccg agagatggct tcgaacacgc tcgtgataat gtcagtggtt 420

gcttcgagat ttacaaatgt atgcgtaccc ggaaacagaa cttggaaatt gatgtcacgg 480

tgattgagtt ctgcacgatg gcgatagtac ctctttgtac tgatcgttga actggtcaag 540

gcccctgcag gaagtacctg tttcgggcta gttgactggc attgagcacc agagtcgaag 600

tttgcccttc aaattgcaac a 621

<210> 533

<211> 944

<212> DNA

<213> Mus musculus picornavirus (strain Wencheng-Mm283)

<400> 533

accccggtaa aggtcagggt gtacttgccc acgttggtcg gcttgtcaac actggttccg 60

gctttatcct gatacgtcac cgtgtactcg ctgccatcga tcacaaaccc ctcatcgtcc 120

ttgacagtga ccgcaggctc ctgcttctct ccgttgaacg tcaccgaggg cggcgtcacc 180

tggatggtgg gcgcggtcac ggtgttgctg atgatggacg catctaccga agcaggtgtg 240

ctgtcattgt ggttgctgtc gccctgggcc ttgtaccaga cagtgtaatt gtccacagct 300

ttcccggtgg gaatggttgg agtatacggg ccgtcctcag ccagagcgta gaccatcgtg 360

ccgtcggaag cggtgcccgc ctcaatcagc gcctgctcgg tgccgttgta gggcagattt 420

tccaaaccct tcggagccgt tgccaccgat gcagcaccct tcgcaatcac aaaggtcccc 480

gtaccgttta cgatgtagtt gccgccgttg gcgttggtga tgatgaccgt cgccgtaccg 540

gcgttgatgt tgtcgcggta ggacagggtg tactcgtcgc tggggatggt gttgccgccg 600

tcctccacct tactgcgaga ctcctagtag ggagtggacg tattttgtgt gtgacccgga 660

gtttcccccg ctccagaaac ctgtgatgag ctatcctcac gcccacctgg caacaggcga 720

ggacgctgcg ttggcacagg acctcattga ggctcacaac ttgctgaagc aaacgaagag 780

acgattgcgc taaggtggtt tcctccggcc tcctgaatgc ggctaatccc atcccttggt 840

catccgccca ctagccagtg ggtagggtgt cgtaacgcgc aagccgaggg cggaaccgac 900

tactttggag tgtccgtgtt tctattttat tttatacggc agtc 944

<210> 534

<211> 541

<212> DNA

<213> Ovine picornavirus

<400> 534

aaaatggggt gcatatagca tggttcccat gctgtatgct accccatttt cttccctccc 60

cctccaacta ccttttcccc ctctattgta acttagaagc taatacacat agcccaatag 120

gggcaccaca cccagtggtg tatagggcaa gcacttctgt ttccccggct ttgccgttaa 180

cccccacagt actacgcgac tgttagtagg taacgtaact agtgaaggtt gatccggagg 240

taaaccccaa cctccagaaa cgtgagatga gggttcagta accccctatg gtaacataga 300

ctgatccctg cgctggctca cgggcttcgt gcactgaatc taggtaaagt tactgaagcc 360

acgagtgtgc tactgtggtt tcctccagcg ccttgaatgc ggctaaccct aaccccggag 420

cacatgcata taatccaata tgtagtgtgt cgtaatgagt aattctggga cgggaccgac 480

tactttaggt tgctgtgttt cctttatttt tatatatcaa tttatggtga caagttacaa 540

g 541

<210> 535

<211> 640

<212> DNA

<213> Pigeon mesivirus 2 (strain pigeon/GALII5-PiMeV/2011/HUN)

<400> 535

ttttgaaaag ggtcccaggg ggatctttta ttccctctgg gcccttttca cgtagtaggg 60

agtggcgccc ctactgcgtt actctgccag gtagtctggg taggtaaacg ggagatcggc 120

ttcgggctta tcttcttaat agcctacgta actgggctga ctggtggggg agctactaga 180

tccgggaaag gactagttac cccttgtaac tctccttcgt gtcaccccat ccacttaccc 240

tttttcccta ccccttagaa tcttaagtag atgtgcaggc ctggcccccc ggaaatgggc 300

aaatcggacg tttgcgtgta gggtcgttct gaaaggtggg gcccactcca ccgtagtagg 360

atcttcctgt ttcacgttta aacctctccg ggcaggtgtc gctagacact aggctgtata 420

ggatggcaag cacttaggtt tcgcaccttc ctagtgcgcc aagaccccgt cttttagttt 480

tagtacatgg ttctttgtaa gatgtctaac cagaggaggt ggtgctgaaa tattgcaagc 540

cactctggcg aacgtccgct gcattgcctg gaacgaactt tctgacgtct agcactggta 600

gcgcggtact agattagtag ggtacctata tggacagggg 640

<210> 536

<211> 460

<212> DNA

<213> Red-necked stint Picornavirus B-like

<400> 536

ctccactctc ctttctccct tccccctccc tttacctttt ccccctctgt aactaagttt 60

tgtccctgca gagcagtgat acgacataac cgagttggaa cgcgtattgg gtattggtga 120

atatctatct catgtggtta agctaaggct tgaaatacct ttggcattgg gttatagtag 180

ggcattagtg ttcccacatg agagaaccat ctatccactt gacacagtgg gtgtatagta 240

caaaggaccc tatttggtat cctgacgcgt gggcgagtaa gtcttgctgg atttccgcga 300

gcatcaacca caccctgata gtctcaatgg ggtcactccc gagacggggc aagtcaccta 360

tacactacct atggttggga agccatagct ggactgtgac agcttgatag agtgtcggcg 420

ggcacagtgg attggatgta aagtccgttg aaatgccatt 460

<210> 537

<211> 412

<212> DNA

<213> Sphenigellan virus

<400> 537

ttctctttta tttaccggtt gacgtcatac ctatgctgct accactgcgc ccggtggggt 60

taactctagg attagcctag agctgtggcc gctaaaagtg acgtaatggt gactaatttg 120

aatgtaacaa tgtagtggtt aagacactca gaggttcaca ttgatcttgt tgggttggta 180

ggctacgggt gaaaccaacc acacctctat attacattaa tatatgtgta tgtgaacgct 240

aaagccctgg ataatgttga gttctataga caataaacat tcaacaggga gtccactggt 300

gtatcatcaa catgccttgt gtttctgatg agtgttgttt ccctcattgc caagttcaat 360

aagccattcc ctatgcgttt agggctactc aggggcattt aggttttgga ca 412

<210> 538

<211> 541

<212> DNA

<213> Sphenimaju virus

<400> 538

tgtttccccc accagtgggt tcaaattccg cccaggcggt atgcctggac ggaatttgga 60

cccactggtg ggggaaacac cagcacgttc taccgtgtgc tacggtggtc atgacaacgt 120

gtggcttgcg ttgtaacgtt tgccgtgttt gacggctaag tatgagtgag aaagaatttt 180

cttctaaatt tcttttactt ttattttccc ggctgtcgtc atgcctatgc tgctaccgtt 240

gcgcccggcg gggttaactc catgattagc atggagctgt ggccgctaaa agtgacgatt 300

tggtactttc tatgggcagg acatgtcagt ataccagaca tgttgcactc gctatgatct 360

ttctgtttgg ggtaggctac gggtgaaacc cctttaggtt accactatgt gggagaacta 420

ctggtacgct aaggccacgg atattggtga gttgttagac aaaaaccatt caacgtggag 480

tccaccggcg gtacaggcag aatgcgtagt ttagtgtttc atttctgaga actgtattct 540

a 541

<210> 539

<211> 843

<212> DNA

<213> Washington bat picornavirus

<400> 539

cctatcgaca gagtttgttg ccgttaaaaa gaggtcccag gggtgtttcc cctggggccc 60

ctttttaacg gcaacaaact ctgtcgatag gctaacgccg agcgttcact tagtgagggc 120

gatttacatc acataacacc gccttgtttg acatgtttca ctcaaggaca agatatggta 180

aatctgtgca ctacttgaaa attctcgacg ttagcggagc taaaacaaca ccacaacaca 240

acacaacaat taacaacaca ttagaaattc gaatttagaa gttagacccc aatttttgat 300

ttaacatctg ttattttgac tttgggcaat ccactgatct ttgaatttca cacgacagcc 360

gtggagagac ggcacacgct acttcagcat agatccaagg atagctggta gcggcgacga 420

agtccacaat cactctatat tttcaggctt ctgaaagtgc tgataaattg attaagttcg 480

gtttatcgga attgccgagg tccctcgaag aaacccgcgg aaatgagaat atagatgttg 540

gtatggcagc cagaagacat gaactatgac aagtacgtac gagcgtgtgt tcggacctgg 600

ttccccgcct taggatcagc gccctgtgag gggacccttt ggaaagatga tcactacata 660

gactactcga acgagaaact atgcaaataa cagggaacgt ttggccaccc ggatgaatcc 720

agtagtacgg aaaagcttag gaattcgatt ttggatcttt tacctgtgtt ttaataagaa 780

atgcaggagt tgataaatag ggctcgactg ggagcgagac tgcagcggct aaagcagcag 840

taa 843

<210> 540

<211> 590

<212> DNA

<213> Waterwitch virus (isolate MW03_1o)

<400> 540

gggctattgc ccggtgactc tttggagccg ccaactggta gttggagcgt agggtcagct 60

ataaggcccc gatttcactt tgggtcgtct ttaccaagtg agcgtttgcc gtgctctacg 120

gctagtgtga ttgtgtagtg tgagtgttat ttctcctgaa ctatctcttg ttttccaggc 180

tgtctccatg ccgatgctgc taccgttgcg cccggcgggg ttaactcaac gactagcgtt 240

gagctgtggc cgctaaaagt ggagatgcag ggactcgtat tggtgtgtgt tggttcagtt 300

ttaacctctt gctgattggc acgcatgatc tggggcggaa ggtaggctac gggtgaaacc 360

ttcctgtatc ggagctccta tggagctgcc gccactaggt acgctaaggc cctagttagg 420

tgatgcttta acgagataag cctcaatggg gagtccacca gcggtactag gcccttgcgt 480

gcgtcccggt tggtaagggg gctggtccag ccctctacca atctatcctg taccctaccc 540

ctcggtttag ggctactcag gggcatgtta ggttttggac aatgatctag 590

<210> 541

<211> 539

<212> DNA

<213> Aphid lethal paralysis virus

<400> 541

ttaaataaga aactatatat tcttacaata tacatttctt gcgcttttaa ggagaaatcc 60

tcaaacctta gacccgtaag acactaagga agaaaaagga aagatttctc tcttgcccca 120

agcccgtcca gctcacgacg tgatataaac ctcagcatta agcccgtcta gctcccgacg 180

tgatataaac gtagcattaa gcccgtctag ctcccgacgt gatataaacg tagcattaag 240

cccgtccagc tcacgacgtg atataaacct cagcaacact ttccaaccaa aacaccaatt 300

agatatttaa gttattttta caattgatta taaatattta ctgcgtacgt ccttgatcgg 360

agctcgtaca gtattcgtac tgatttgcac aaatcttcgc agtgttattg gcttcttttt 420

gcctaaagtt ttgctattga tatttacact ctttttcttt tgcggtttag taaataagaa 480

gagttattta tcttgtttgt ttttgattta cgcatttgac acttataaaa ctatacaaa 539

<210> 542

<211> 708

<212> DNA

<213> Cricket paralysis virus

<400> 542

tttaataagt gttgtgcaga ttaatctgca cagcactagc cacaaagttc agtggtccca 60

attatgggat ataaaactca atggtcgacg gagtgacctg gaaatgctcc ccgtgagaaa 120

ccttgtttaa acgcacttgg accttctccg gcctcagcgc caggcatagc tggatcgtca 180

atcccaagtt tggacttaga cttgctctat gttacaattg ggaggagcgt gacttaccat 240

tggtcgttga tgacgttaat tccaaacaaa aacaacaaca acaacttagt ttaaataatt 300

ccggtcttta agcatcaggg tagaccgatg aggaataaag aaccctataa gactgattcg 360

ataccaagag ctggtgtctg agggattacg actcgcgtgg tcggacagca catgttagtc 420

atgtattatg gtgtttccca acacgtatct ttgctctagt attgaacttt agtgatgagt 480

gtacttggca tgtacacctg attagttagc ccttgtggat acgccactgc cgctcaggta 540

cagtaggaca taccccaaat attgggttcc actcgtagag aagaaaatta gtggcgaaac 600

gtacggttta tgtcaaagga gaaggtgatt ttaattgcac ctgaagcaac cctaggtctt 660

ccctttgatg tgagctaata aggagtaacc gattcttaca atgtgatc 708

<210> 543

<211> 798

<212> DNA

<213> Drosophila C virus (strain EB)

<400> 543

tttatatcgt gtgtacatat aaatatgtac acacggcttt taggtagaat attgttttca 60

atgttgattt taaaggtaac tttggttatt atgctttacg gttttcattg ttgatggtat 120

ttgtggcctg cggtccctaa ttgttgaatt atttattctg atacgttgtt ttcattgttg 180

atggtaagga ttcttatttt gaagtggttt ttcagaagat aactctaaat atgaattatg 240

ccttattgtt ttcaatgttg atggccttcg tttaaatact ctttgttaat gacggtaatc 300

aaagattaca tctcaaactt agattaatat ttttaagtag ggtatactga gttagtcctc 360

tctctttact gattttgata tctggtaatt gacttcgaag aaagatgcgt cttttggatt 420

tgtaatgact gggccttaag ttcataggtg ttattacatg gaggaacaca ttactttggt 480

tgatgatgat gttttgatga tgactttcaa tgtatgtgct tatgttaagc ctgacataag 540

aacttactag tttgcataat gcaaagggtt agtatatgat ttttagtatg tggattttga 600

cactgccttt gattaggatg tgtgaatgat tttgaaacat attaagatgt ttatacgagc 660

gtgttgttta ctattttcag gatatgtgga agcggttgtg tatgatctat acgcacattt 720

agttcccaga gggcgttgtc gtctccccct aagcaaggga gaaacacgtg gcacatgatc 780

ttgcgcttaa cgataaaa 798

<210> 544

<211> 419

<212> DNA

<213> Homalodisca coagulata virus-1

<400> 544

caaaaattgt acgcagcaaa cactgcgtac aatgagtagc atgactaaga tggagagttc 60

ctaacccggc tctcaaatta gtgatgcaat acttaagtag tagaaacgaa agagttagca 120

gatgatcgaa caaacatcta agtcaaacca aaaacccaac ccaacaaaca caaccgttag 180

catatcccaa aagttttata gtaccaacta gaaatagaga gatgataaaa ctttattgct 240

aacacattga cgttacggac aacccgacac ggactgaaca ggagcatgtt agacatgtat 300

tatggctccg gaagatagaa aatatgctta catccccatg gtaggtaaga tttctataac 360

tactggccac tgaaaagtga atgcagtagg cggcaacccc gcatccaaac ttcaaccaa 419

<210> 545

<211> 883

<212> DNA

<213> Antheraea pernyi iflavirus (isolate LnApIV-02)

<400> 545

tccgagattt gttttttggc gattttgtgt gtaacaaact ttgacagctt actatttaat 60

ttaattaact ttactttata acatttagtt tcaaaatata aacttaattt aaagattttt 120

atgtattata ttttatagta ttattaaaat ataaaaactc ggtactgacc ggggttgtat 180

ctaacaatta ataaaagcta tataatgtgt tttcactatc ggcctatctt attagtcttt 240

tagtagcata gataaatagt gacactggca taatcacccc cagtggcaac acgcacctct 300

tatatttctt ttgttaaaat gatttacaat gtataaataa taactttaag gcaaggtaca 360

gctacgtcct cgatgtttat aagtatcata tgaagtgcta atatagattt gcaaaagccg 420

tttgttttaa tttaacgtca cctctaagga gtgcgtgtcg actttaaatc ttcggtgtaa 480

tttagtccga tctataaacc ccaagtaata tgtgaaagta accgctcagg cgtatgtgtg 540

tatgacgrta ttctggtctc tggaatatca taattcgtgc ttggctttgt ccaaatcctc 600

gagagcgtac gaaccaacgg tcatcgggaa tatacttgtg aacgaataaa gtagtcatat 660

aagtatgcac gaactttggt ctcctaattt ctggaagttt tgtaggaaga taaagcttac 720

cactccttta tattctttct ttttatggtg acaattttgc aagtaatttt attyacagtg 780

tttctcattg ctattaatat tcaatttcca ggtatcactt ttcatttgca ttcgattttc 840

ttttactttt atttcaacaa tttgaatatc caattaatca aat 883

<210> 546

<211> 551

<212> DNA

<213> Isla virus (strain Cx 1773-5)

<400> 546

taccccagat tagtaacaaa atcataaaac tcggtacaga ccggggcaac tagttctggt 60

tatttaataa tttgtttgac cttatcttat gttcaatgcc gtgttcgctt gcgtctttaa 120

accccaaaag atgttcccca taagttttca aattattatt taattagtgg ttgtgtataa 180

atacttattt aaggcgaggc actttatgat ttaatgttta taagtaagcg gatttctgtt 240

agcacgagtt taatactatg gtgaccaccg actagtaatc gagtacttta gatataaggc 300

caaaagccct ctatatcaca tatgtagtgt gttagtaaag ataacagaga gaagccgaaa 360

gtaaccgctt aatgattaag agtgtgacag aagaagcttt cttcttaaat catttcgcat 420

tgattgtgta attcgataga tgtgtatctc tacctatcgg atgtatcttt ctaaaagata 480

gatgatctta gaaccatctt ttgactgttt tatgtgtaat aaaattgtca ttccttttca 540

acttttcaac t 551

<210> 547

<211> 886

<212> DNA

<213> Chaetoceros socialis f. radians RNA virus

<400> 547

agataaatac gggtgtagtg cagcttttgc tgcactacac tcgtattgat ggatttcgcg 60

ctctaccgcg ccgttttgct tattgaatgt tttattactt ctggatctca ggcaagactg 120

cctgttattc gttgtttagt tcgttttcta agcataatgt acgaaattta tcaagtgtga 180

tttgagaata aaactcaacg ccctatatct acaaacgatt atgataaatt gaatctcccc 240

ctatctgtta tttagattgt ttgattttct ttcaactttc gacaactata cagttatatt 300

atgggaaatc tagttgtatt ataaatttga atttaactag ctcaatataa ttaagattta 360

tatattgata ttatttattg actgattcac cagagtcttt catgtgaact ctatgttcta 420

catcatgttt tattcacatc cccttccgag gtgatgttaa acaccatgtc attatggaaa 480

aatgaccaga gatagcctat aagaataaac tctgtttaaa ctcttttaga tgcatggctt 540

gccctgtcta atcgtatttc agcctaggtg actggctata taattaaagc gttaccacat 600

ccaaataaga tgggatctcc ggatcacatc gcctgttaat atcataagat attgtaaact 660

ttatctctta ggagattgta ggtggtgagg gcttaatgac cccaataccc acgactacca 720

ctttatgtga aagcacttta tcacgaaatt agcgtgatgg attatctgag acgatgtcca 780

cataatgcgc tttgagtaga ttatggtatg cctgaattga tcacttccaa gattttcttt 840

tcttggtatc gatcttttca gacaggcaat tggactttaa tatatt 886

<210> 548

<211> 384

<212> DNA

<213> Apple latent spherical virus

<400> 548

tccgttttag agcttgtgag ttgttattac gttttgttgt ttgaatctct gttttgttac 60

tctgatttcc ttcacgtttg caccttacac tctctaccga taaacattat catcttttat 120

tttgaatttt atttgaaaaa tttttatatt ttcaatgtca atcactactc ctgtagtgat 180

tcgctttggg agttttgact cccctgctgc ggcacagatt gccgatacta aggaggagga 240

aactcctctc tctcgccttg cagctttaaa tcgcaaggct caaattgctg ctcgttcaag 300

agtagcagct gctgcggcac agtatgccga agagcaggag acactagttc tcactgctca 360

atccatgagg gatgctctcc cttt 384

<210> 549

<211> 50

<212> DNA

<213> Artificial

<220>

<223> sequence of 5'spacer

<400> 549

aaaaaacaaa aacaaaaaaa acaaaaaaac aaaaaaaaaa ccaaaacaca 50

<210> 550

<211> 50

<212> DNA

<213> Artificial Sequence

<220>

<223> sequence of 5'spacer

<400> 550

aaaaacaaaa aacaaaaaaa aaaccaaaaa aacaaaaaaa acaaaacaca 50

<210> 551

<211> 25

<212> DNA

<213> Artificial Sequence

<220>

<223> sequence of 3'spacer

<400> 551

aaaaaaacaa aaaaacaaaa caaac 25

<210> 552

<211> 22

<212> DNA

<213> Artificial Sequence

<220>

<223> sequence of 3'spacer

<400> 552

aaaaacaaaa aacaaaacaa ac 22

<210> 553

<211> 19

<212> DNA

<213> Artificial Sequence

<220>

<223> sequence of 3'spacer

<400> 553

aaaaaacaaa aaacaaaac 19

<210> 554

<211> 16

<212> DNA

<213> Artificial Sequence

<220>

<223> sequence of the first exon

<400> 554

agacgctacg gactta 16

<210> 555

<211> 51

<212> DNA

<213> Artificial Sequence

<220>

<223> sequence of the second exon

<400> 555

aaaatccgtt gaccttaaac ggtcgtgtgg gttcaagtcc ctccaccccc a 51

<210> 556

<211> 114

<212> DNA

<213> Artificial Sequence

<220>

<223> sequence of 5'intron

<400> 556

aataattgag ccttaaagaa gaaattcttt aagtggatgc tctcaaactc agggaaacct 60

aaatctagtt atagacaagg caatcctgag ccaagccgaa gtagtaatta gtaa 114

<210> 557

<211> 131

<212> DNA

<213> Artificial Sequence

<220>

<223> sequence of 3'intron

<400> 557

aacaatagat gacttacaac taatcggaag gtgcagagac tcgacgggag ctaccctaac 60

gtcaagacga gggtaaagag agagtccaat tctcaaagcc aataggcagt agcgaaagct 120

gcaagagaat g 131

<210> 558

<211> 21

<212> DNA

<213> Artificial Sequence

<220>

<223> sequence of 5'homology arm

<400> 558

accgtcagtt gctcactgtg c 21

<210> 559

<211> 21

<212> DNA

<213> Artificial Sequence

<220>

<223> sequence of 5'homology arm

<400> 559

accgtgctat gtccacgtgt c 21

<210> 560

<211> 21

<212> DNA

<213> Artificial Sequence

<220>

<223> sequence of 3'homology arm

<400> 560

gcacagtgag caactgacgg a 21

<210> 561

<211> 21

<212> DNA

<213> Artificial Sequence

<220>

<223> sequence of 3'homology arm

<400> 561

gacacgtgga catagcacgg a 21

<210> 562

<211> 741

<212> DNA

<213> Coxsackievirus B3 (CVB3)

<400> 562

ttaaaacagc ctgtgggttg atcccaccca caggcccatt gggcgctagc actctggtat 60

cacggtacct ttgtgcgcct gttttatacc ccctccccca actgtaactt agaagtaaca 120

cacaccgatc aacagtcagc gtggcacacc agccacgttt tgatcaagca cttctgttac 180

cccggactga gtatcaatag actgctcacg cggttgaagg agaaagcgtt cgttatccgg 240

ccaactactt cgaaaaacct agtaacaccg tggaagttgc agagtgtttc gctcagcact 300

accccagtgt agatcaggtc gatgagtcac cgcattcccc acgggcgacc gtggcggtgg 360

ctgcgttggc ggcctgccca tggggaaacc catgggacgc tctaatacag acatggtgcg 420

aagagtctat tgagctagtt ggtagtcctc cggcccctga atgcggctaa tcctaactgc 480

ggagcacaca ccctcaagcc agagggcagt gtgtcgtaac gggcaactct gcagcggaac 540

cgactacttt gggtgtccgt gtttcatttt attcctatac tggctgctta tggtgacaat 600

tgagagatcg ttaccatata gctattggat tggccatccg gtgactaata gagctattat 660

atatcccttt gttgggttta taccacttag cttgaaagag gttaaaacat tacaattcat 720

tgttaagttg aatacagcaa a 741

<210> 563

<211> 742

<212> DNA

<213> human echovirus 29 strain jv-10e29

<400> 563

ttaaaacagc ctgtgggttg atcccaccca cagggcccac tgggcgctag cactctggta 60

tcacggtacc tttgtgcgcc tgttttatac ttcctccccc aactgcaact tagaagtaac 120

acaaaccgat caacagtcag cgtggcacac cagccacgtt ttgatcaaac acttctgtta 180

ccccggactg agtatcaata gactgctcac gcggttgaag gagaaaacgt tcgttatccg 240

gccaactact tcgagaaacc tagtaacgcc atggaagttg tggagtgttt cgctcagcac 300

taccccagtg tagatcaggt tgatgagtca ccgcattccc cacgggtgac cgtggcggtg 360

gctgcgttgg cggcctgccc atggggaaac ccatgggacg ctcttataca gacatggtgc 420

gaagagtcta ttgagctagt tggtagtcct ccggcccctg aatgcggcta atcccaactg 480

cggagcatac actctcaagc cagagggtag tgtgtcgtaa tgggcaactc tgcagcggaa 540

ccgactactt tgggtgtccg tgtttcattt tattcctata ctggctgctt atggtgacaa 600

ttgagagatt gttaccatat agctattgga ttggccatcc ggtgactaac agagctatta 660

tatatctttt tgttgggttt ataccactta gcttgaaaga ggttaaaact ctacattaca 720

ttttaatact gaacaccgca aa 742

<210> 564

<211> 742

<212> DNA

<213> human poliovirus 1 strain mahoney_cdc 5 utr

<400> 564

ttaaaacagc tctggggttg tacccacccc agaggcccac gtggcggcta gtactccggt 60

attgcggtac ccttgtacgc ctgttttata ctcccttccc gtaacttaga cgcacaaaac 120

caagttcaat agaagggggt acaaaccagt accaccacga acaagcactt ctgtttcccc 180

ggtgatgtcg tatagactgc ttgcgtggtt gaaagcgacg gatccgttat ccgcttatgt 240

acttcgagaa gcccagtacc acctcggaat cttcgatgcg ttgcgctcag cactcaaccc 300

cagagtgtag cttaggctga tgagtctgga catccctcac cggtgacggt ggtccaggct 360

gcgttggcgg cctacctatg gctaacgcca tgggacgcta gttgtgaaca aggtgtgaag 420

agcctattga gctacataag aatcctccgg cccctgaatg cggctaatcc caacctcgga 480

gcaggtggtc acaaaccagt gattggcctg tcgtaacgcg caagtccgtg gcggaaccga 540

ctactttggg tgtccgtgtt tccttttatt ttattgtggc tgcttatggt gacaatcaca 600

gattgttatc ataaagcgaa ttggattggc catccggtga aagtgagatt cattatctat 660

ctgtttgctg gattcgctcc attgagtgtg tttactctaa gtacaatttc aacagttatt 720

tcaatcagac aattgtatca ta 742

Claims

1. A method of screening IRES based on levenstein distance, comprising the steps of:

(3) Traversing the sample sequences, and calculating the Levenstan distance between each sample sequence and the sequence to be predicted;

(5) Determining whether the sequence to be predicted includes an IRES based on the mean.

2. The method for screening for IRES based on the Levensitan distance of claim 1, wherein in the step (5), if the mean value is not less than the set prediction threshold, it is determined that the sequence to be predicted contains an IRES, otherwise it is determined that the sequence to be predicted is not listed as containing an IRES.

3. The method of levens distance-based screening of IRES according to claim 2, wherein the prediction threshold is not less than 0.5; preferably, the prediction threshold is 0.75.

4. The method for levensan distance-based screening of IRES according to any of claims 1-3, wherein the method further comprises the steps of: and carrying out experimental verification on the sequence to be predicted which is judged to contain the IRES so as to verify the IRES activity of the sequence to be predicted.

5. The method of levensan distance-based screening for IRES according to claim 4, wherein the step of experimentally validating comprises:

constructing a circular nucleic acid molecule in which a sequence to be predicted is operably linked to a nucleotide sequence encoding a fluorescent protein using a sequence to be predicted determined to comprise an IRES;

6. A polynucleotide, wherein the polynucleotide is selected from at least one of the group consisting of:

(ii) (ii) a mutant sequence of any one of the nucleotide sequences set forth in (i) which has a mutated nucleotide at one or more positions corresponding to any one of the nucleotide sequences set forth in (i) and which has the translational activity of the starting circular nucleic acid molecule;

(iii) (iii) a nucleotide sequence which is reverse complementary to the hybridizing sequence of the nucleotide sequence set forth in (i) or (ii) and which has the translation activity to initiate the circular nucleic acid molecule under high stringency hybridization conditions or very high stringency hybridization conditions;

(iv) (iii) a nucleotide sequence having at least 70%, optionally at least 80%, preferably at least 90%, more preferably at least 95%, most preferably at least 98% sequence identity with the nucleotide sequence set forth in either (i) or (ii) and which has the translational activity of the initiating circular nucleic acid molecule.

7. The polynucleotide of claim 6, wherein the polynucleotide is a polynucleotide comprising an IRES screened according to the method of any one of claims 1-5.

8. The polynucleotide according to claim 6 or 7, wherein (a) is as follows ₁ )-(a ₂ ) Use in at least one of:

9. A circular nucleic acid molecule, wherein the circular nucleic acid molecule comprises the polynucleotide of claim 6 or 7;

10. A circularized precursor nucleic acid molecule, wherein said circularized precursor nucleic acid molecule is circularized to form a circular nucleic acid molecule of claim 9;

optionally, the circularized precursor nucleic acid molecule further comprises one or more elements of:

11. A recombinant nucleic acid molecule, wherein said recombinant nucleic acid molecule is selected from the group consisting of (f) ₁ )-(f ₂ ) Any one of:

(f ₁ ) Comprising the polynucleotide of claim 6 or 7,

(f ₂ ) Transcribing to form the circularized precursor nucleic acid molecule of claim 10.

12. A recombinant expression vector comprising the recombinant nucleic acid molecule of claim 11.

13. A recombinant host cell comprising the polynucleotide of claim 6 or 7, the circular nucleic acid molecule of claim 9, the circularized precursor nucleic acid molecule of claim 10, the recombinant nucleic acid molecule of claim 11, or the recombinant expression vector of claim 12.

14. A method of making a circular nucleic acid molecule with an increased level of protein expression, wherein the method comprises the step of operably linking the polynucleotide of claim 6 or 7 to a coding region of the circular nucleic acid molecule.

15. The circular nucleic acid molecule of claim 9, the circularized precursor nucleic acid molecule of claim 10, the recombinant nucleic acid molecule of claim 11, or the recombinant expression vector of claim 12 in (g) ₁ )-(g ₃ ) Use in at least one of:

(g ₃ ) As or in the preparation of nucleic acid vaccines;