CN115943963B - Sterilization composition containing methiadin and dichlobentiazox and application thereof - Google Patents

Sterilization composition containing methiadin and dichlobentiazox and application thereof Download PDF

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Publication number
CN115943963B
CN115943963B CN202211657463.6A CN202211657463A CN115943963B CN 115943963 B CN115943963 B CN 115943963B CN 202211657463 A CN202211657463 A CN 202211657463A CN 115943963 B CN115943963 B CN 115943963B
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dichlobentiazox
bactericidal composition
methiadin
agents
agent
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CN115943963A (en
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许艳秋
罗杰
梁灵
曹棣
刘军
杨志兰
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Sichuan Institute Of Pesticide Control
Sichuan Lier Crop Science Co Ltd
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Sichuan Institute Of Pesticide Control
Sichuan Lier Crop Science Co Ltd
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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Abstract

The invention belongs to the field of agricultural bactericides, and provides a bactericidal composition containing methiadin and dichlobentiazox and application thereof, wherein the mass ratio of the methiadin to Dichlobentiazox is 80:1-1:80; the bactericidal composition has excellent synergistic effect on crop diseases; can effectively reduce the dosage of pesticides, slow down the generation of drug resistance of diseases, reduce environmental pollution and ensure that the field observation is safe to crops.

Description

Sterilization composition containing methiadin and dichlobentiazox and application thereof
Technical Field
The invention belongs to the field of agricultural bactericides, and particularly relates to a bactericidal composition containing methiadin and dichlobentiazox and application thereof.
Background
The chemical agent is used for preventing and controlling crop diseases, has the advantages of obvious prevention effect, convenience, simplicity and rapidness in use, but chemical prevention and control can cause harm to life safety of people and livestock, residue after pesticide use causes environmental pollution, and disease resistance is gradually enhanced after long-term use of a single agent. The pesticide compounding is a bactericide mode for reducing the occurrence of disease drug resistance, in particular to a bactericidal composition with synergistic effect, which can delay or overcome the occurrence of drug resistance after screening harmful organisms by pesticides, reduce cost and further alleviate the environmental safety problem in pesticide use.
The mefenacet is a thiadiazole plant disease-preventing activator which is developed together by the university of south China. CN100420686C discloses a synthesis method of methiadin, and also discloses an excellent control effect on cucumber fusarium wilt, wheat scab, rice aspergillus, sclerotinia rot, apple canker, banana anthracnose, cucumber anthracnose, cotton verticillium and potato late blight; CN101091490a discloses that it has excellent control effect on cucumber downy mildew. The current research results show that the compound has good disease resistance induction effect on viral diseases, and the action mechanism is to induce plant to generate systematic and broad-spectrum disease resistance activity.
Dichlobentiazox is benzothiazole bactericide, is mainly used for preventing and treating rice diseases, has good prevention effect on rice blast and banded sclerotial blight in particular, and has excellent prevention and treatment effect on cucumber anthracnose.
The existing improper use of pesticides causes stronger and stronger disease resistance to pesticides, so that the use amount of pesticides is increased year by year, and the environmental pollution is more and more serious. Therefore, rational use of pesticides and bactericides for preparing the compositions for enlarging the spectrum of sterilization and reducing the resistance to diseases has been eagerly made. There is no report in the prior art on the compounding of methiadinamine with Dichlobentiazox.
Disclosure of Invention
The invention provides a bactericidal composition with excellent synergism, which comprises the following specific technical scheme:
A bactericidal composition containing the methientide and Dichlobentiazox comprises the active ingredients of the methientide and Dichlobentiazox, wherein the mass ratio of the methientide to Dichlobentiazox is 80:1-1:80.
The mass ratio of the methiadin to Dichlobentiazox in the bactericidal composition is 40:1-1:20.
The mass ratio of the methiadin to Dichlobentiazox in the bactericidal composition is 20:1-1:1.
The bactericidal composition comprises 1-80% of auxiliary agent and active ingredient by weight percent. Preferably the active ingredient is present in an amount of 10 to 60% by weight.
The auxiliary agent is one or more of wetting agent, emulsifying agent, dispersing agent, suspending agent, antifreezing agent, preservative, thickening agent, defoamer, solvent, cosolvent and filler.
The preparation of the bactericidal composition is missible oil, oil suspending agent, water suspending agent, wettable powder, water dispersible granule, microemulsion, aqueous emulsion and granule.
The bactericidal composition is used for preventing and controlling crop diseases.
The crop diseases comprise cucumber anthracnose, rice sheath blight, rice blast, cucumber downy mildew, cucumber fusarium wilt, wheat scab, rice false smut, sclerotinia rot, apple rot, banana anthracnose, cotton verticillium, potato late blight and tobacco mosaic virus. Preferably, the crop disease is cucumber downy mildew.
By adopting the technical scheme, the invention has the following beneficial effects:
When the compounding mass ratio of the methiadin and Dichlobentiazox is 40:1-1:20, the composition shows remarkable synergistic effect on cucumber anthracnose bacteria; when the compound mass ratio of the methiadin to Dichlobentiazox is 20:1-1:1, the composition shows remarkable synergistic effect on the Rhizoctonia solani. The composition can effectively reduce the dosage of pesticides when the composition shows synergistic effect, slow down the generation of drug resistance of diseases and reduce environmental pollution. And field observation is safe to crops
Detailed Description
The invention is further illustrated with reference to specific embodiments.
Example 1: indoor toxicity test of cucumber anthracnose pathogen by combining methiadin and Dichlobentiazox
Test materials:
cucumber anthracnose pathogen (Colletotrichum lagenarium); and (5) field collection and identification, and indoor culture and preservation.
The testing method comprises the following steps:
The hypha growth rate method was used for measurement. A series of concentration gradients of drug-containing PDA culture media were prepared on a pre-experimental basis. Cutting bacterial blocks along the edges of bacterial colonies by using a puncher with the diameter of 5mm, respectively placing the bacterial blocks on medicine-containing culture mediums with different concentration gradients, placing the bacterial blocks in an incubator for culture, measuring the diameters of the bacterial colonies by using a crisscross when the bacterial colonies of a control group grow to 4/5 of a culture dish, and calculating the growth inhibition rate of hyphae. And (3) carrying out corresponding regression analysis according to the concentration of each single agent and the composition and the corresponding control effect, and obtaining the EC 50 value of each agent.
Bacteriostasis% = (control group colony diameter-treatment group colony diameter)/(control group colony diameter-5) ×100%.
And calculating the co-toxicity coefficient (CTC) of the mixture by using a co-toxicity coefficient method calculated by grand cloud Peel, and determining the synergy of the mixture.
Single dose toxicity index= (EC 50 for standard dose/EC 50 for certain single dose) ×100;
theoretical Toxicity Index (TTI) =toxicity index of a single dose×proportion of a single dose in the mixture+toxicity index of B single dose×proportion of B single dose in the mixture;
Measured virulence index (ATI) = (EC 50 value for standard single dose/EC 50 value for cocktail) ×100;
co-toxicity coefficient (CTC) = (measured virulence index/theoretical virulence index) ×100;
The compound has a synergistic effect when CTCs are greater than 120, antagonism when CTCs are less than 80, and additive effect between 80 and 120.
Test results
As can be seen from Table 1, the EC 50 value 3.2815mg/L for the B.cucumeris and the EC 50 value 0.2408mg/L for the B.cucumeris of Dichlobentiazox; when the compounding mass ratio of the methiadin and Dichlobentiazox is 40:1-1:20, the composition shows remarkable synergistic effect on cucumber anthracnose bacteria, and the CTC value is more than 120; when the compounding mass ratio of the methiadin and Dichlobentiazox is 80:1, 1:40-1:80, the composition shows additive effect on cucumber anthracnose.
Table 1: indoor test of cucumber anthracnose pathogen by compounding methiadin and Dichlobentiazox
Example 2: indoor toxicity test of rice sheath blight pathogen by compounding methiadin and Dichlobentiazox
Test materials:
Rice sheath blight germ (Rhizoctonia solani); and (5) field collection and identification, and indoor culture and preservation.
The testing method comprises the following steps:
The hypha growth rate method was used for measurement. A series of concentration gradients of drug-containing PDA culture media were prepared on a pre-experimental basis. Cutting bacterial blocks along the edges of bacterial colonies by using a puncher with the diameter of 5mm, respectively placing the bacterial blocks on medicine-containing culture mediums with different concentration gradients, placing the bacterial blocks in an incubator for culture, measuring the diameters of the bacterial colonies by using a crisscross when the bacterial colonies of a control group grow to 4/5 of a culture dish, and calculating the growth inhibition rate of hyphae. And (3) carrying out corresponding regression analysis according to the concentration of each single agent and the composition and the corresponding control effect, and obtaining the EC 50 value of each agent.
Bacteriostasis% = (control group colony diameter-treatment group colony diameter)/(control group colony diameter-5) ×100%.
And calculating the co-toxicity coefficient (CTC) of the mixture by using a co-toxicity coefficient method calculated by grand cloud Peel, and determining the synergy of the mixture.
Single dose toxicity index= (EC 50 for standard dose/EC 50 for certain single dose) ×100;
theoretical Toxicity Index (TTI) =toxicity index of a single dose×proportion of a single dose in the mixture+toxicity index of B single dose×proportion of B single dose in the mixture;
Measured virulence index (ATI) = (EC 50 value for standard single dose/EC 50 value for cocktail) ×100;
co-toxicity coefficient (CTC) = (measured virulence index/theoretical virulence index) ×100;
The compound has a synergistic effect when CTCs are greater than 120, antagonism when CTCs are less than 80, and additive effect between 80 and 120.
Test results
As can be seen from Table 2, the EC 50 value 21.4434mg/L for sheath blight of rice, and the EC 50 value 0.8096mg/L for sheath blight of rice, as per Dichlobentiazox, of methiphamine; when the compound mass ratio of the methiadin to Dichlobentiazox is 20:1-1:1, the composition shows remarkable synergistic effect on the Rhizoctonia solani, and the CTC value is more than 120; when the compounding mass ratio of the methiadin and Dichlobentiazox is 80:1-40:1 and 1:10-1:80, the composition shows additive effect on the sheath blight germ of rice.
Table 2: indoor test of sheath blight germ of rice by compounding methiadin and Dichlobentiazox
Example 3 field control of Rice blast by the combination of Methylthialuring amine and Dichlobentiazox
Testing crop disease: rice blast of rice
Testing the medicament:
30% of suspending agent (comprising the following components by weight percent of methiadin 25%, dichlobentiazox%, calcium dodecyl benzene sulfonate 5%, sodium lignin sulfonate 3%, NNO 2%, glycerol 2%, magnesium aluminum silicate 1.5%, sodium benzoate 0.5%, silicone defoamer silicone oil 0.5%, bentonite 1% and water for 100%);
25% of suspending agent (comprising the following components by weight percent of methiadin 25%, calcium dodecyl benzene sulfonate 5%, sodium lignin sulfonate 3%, NNO 2%, glycerol 2%, magnesium aluminum silicate 1.5%, sodium benzoate 0.5%, silicone oil 0.5%, bentonite 1% and water 100%);
5% suspending agent (comprising the components of Dichlobentiazox% by weight, 5% by weight of calcium dodecyl benzene sulfonate, 3% by weight of sodium lignin sulfonate, 2% by weight of NNO 2, 2% by weight of glycerol, 1.5% by weight of magnesium aluminum silicate, 0.5% by weight of sodium benzoate, 0.5% by weight of silicone oil as silicone defoamer, 1% by weight of bentonite and 100% by weight of water);
The test method comprises the following steps: reference to pesticide field efficacy test criterion (one) bactericides for preventing and controlling rice leaf diseases. Cell setting: each cell area was 50m 2, random block arrangement, and each treatment was repeated 4 times. Spraying the medicament for three times respectively on the stem and leaf of the rice in the jointing stage, the booting stage and the breaking stage, wherein the spraying amount is 40 kg/mu; the investigation was started 30d after the third dose.
The investigation method comprises the following steps: 5 points are randomly taken for investigation in each cell, 50 plants are investigated in each point, and each plant is used for investigating flag leaves and two leaves below the flag leaves.
Leaf blast classification standard:
Level 0: no disease;
stage 1: less than 5 leaf spots and less than 1cm in length;
3 stages: 6-10 leaf spots with the length more than 1cm;
5 stages: 11-25 leaf lesions, part of the lesions are connected into a piece, and the leaf area is 10-25%;
7 stages: more than 26 leaf spots are connected into a piece, and the leaf area is 26-50%;
Stage 9: the disease spots are connected into pieces, and occupy more than 50% of the leaf area or all dead.
And (3) calculating the drug effect:
Disease index = Σ [ leaf number of each stage x relative-stage number ] ×100/[ total leaf number of investigation x highest-stage representative value ]
Control effect (%) = [ (control area disease index-treatment area disease index)/control area disease index ] ×100
Test results
As shown in Table 2, the control effect of the 30% suspending agent on rice blast is 92.0% compared with the single agent; the compound medicament has obviously improved control effect on rice blast, can effectively reduce the use amount of active ingredients, reduce environmental pollution and slow down the generation of disease drug resistance; and the added observation is safe for crops.
Table 3: field control effect test of compound medicament on rice blast
Example 4 formulation example
The agent of the present invention may also be configured as follows:
wettable powder:
comprises the following components in percentage by weight: 1-40% of methiadin, 1-40% of Dichlobentiazox%, 1-10% of dispersing agent, 1-10% of wetting agent and 100% of filler. Mixing the methidatura amine, dichlobentiazox, a dispersing agent, a wetting agent and a filler, uniformly mixing in a mixing tank, crushing by a jet mill, and uniformly mixing to obtain the methidatura amine Dichlobentiazox wettable powder product.
Water dispersible granule:
comprises the following components in percentage by weight: 1-40% of methiadin, 1-40% of Dichlobentiazox%, 1-10% of dispersing agent, 1-10% of wetting agent, 1-5% of disintegrating agent, 1-5% of binding agent and 100% of filler. The methiadin, dichlobentiazox, dispersing agent, wetting agent, disintegrating agent, binder, filler and the like are crushed together by air flow to obtain the required particle size, thus obtaining the granulating material. Quantitatively feeding the materials into a fluidized bed granulating dryer for granulating and drying to obtain the methiadin-Dichlobentiazox water dispersible granule product.
Aqueous emulsion:
The composition comprises the following components in percentage by weight: 1-40% of methiadin, 1-40% of Dichlobentiazox%, 1-20% of solvent and cosolvent, 1-10% of emulsifier, 0.5-5% of antifreezing agent, 0.1-2% of thickening agent, 0.1-2% of defoaming agent and the balance of deionized water. Mixing the above formula materials, and adding the methiadin technical, dichlobentiazox technical, solvent and emulsifier together to dissolve into uniform oil phase; deionized water, an antifreeze agent, a thickening agent and a defoaming agent are mixed together to form a uniform water phase. Under high-speed stirring, adding the water phase into the oil phase to obtain the methiadinamine Dichlobentiazox aqueous emulsion.
Microemulsion:
the composition comprises the following components in percentage by weight: 1-40% of methiadin, 1-40% of Dichlobentiazox%, 1-30% of solvent and cosolvent, 5-30% of emulsifier, 0.5-5% of antifreezing agent, 0.1-2% of defoaming agent and the balance of deionized water. Dissolving a methidathimide crude drug and Dichlobentiazox crude drugs in a reaction kettle filled with a solvent and a cosolvent; adding the emulsifier, the anti-freezing agent, the defoaming agent and the deionized water into a reaction kettle filled with the solution, and then intensively mixing and homogenizing to obtain the transparent and clear methiadinamide Dichlobentiazox microemulsion.
Finally, it should also be noted that the above list is merely a few specific embodiments of the present invention. All modifications which can be directly derived or suggested by the person skilled in the art from the teaching of the present disclosure are considered to be within the scope of the present invention.

Claims (7)

1. The bactericidal composition containing the methientide is characterized in that the active ingredients consist of the methientide and Dichlobentiazox, and the mass ratio of the methientide to Dichlobentiazox is 40:1-1:20.
2. The bactericidal composition of claim 1, wherein the mass ratio of the methiadin to Dichlobentiazox is 20:1-1:1.
3. A bactericidal composition according to any of claims 1-2, characterized in that the bactericidal composition contains adjuvants in an amount of 1-80% by weight of active ingredient.
4. A bactericidal composition according to claim 3, wherein the active ingredient is present in an amount of 10 to 60% by weight.
5. A bactericidal composition according to claim 3, wherein the adjuvant is one or more of wetting agents, emulsifying agents, dispersing agents, suspending agents, anti-freeze agents, preserving agents, thickening agents, antifoaming agents, solvents, co-solvents, fillers.
6. The bactericidal composition of claim 5, wherein the bactericidal composition is in the form of an emulsifiable concentrate, an oil suspension, an aqueous suspension, a wettable powder, a water dispersible granule, a microemulsion, an aqueous emulsion, or a granule.
7. The use of the bactericidal composition according to claim 2 for controlling crop diseases, wherein the crop diseases are colletotrichum cucumerinum and banded sclerotial blight.
CN202211657463.6A 2022-12-22 2022-12-22 Sterilization composition containing methiadin and dichlobentiazox and application thereof Active CN115943963B (en)

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Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106538553A (en) * 2016-09-30 2017-03-29 赵青娇 A kind of bactericidal composition containing probenazole and preparation

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CN107094776A (en) * 2017-05-06 2017-08-29 北京科发伟业农药技术中心 Bactericidal composition containing isotianil
CN111165498A (en) * 2018-11-12 2020-05-19 东莞市东阳光菌阳氢专利农药有限公司 Bactericidal composition containing Dichlorobentiazox and fluorophenyletheramide, and application and preparation method thereof
CN109452287A (en) * 2018-11-14 2019-03-12 东莞东阳光科研发有限公司 A kind of bactericidal composition and application thereof and preparation method

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106538553A (en) * 2016-09-30 2017-03-29 赵青娇 A kind of bactericidal composition containing probenazole and preparation

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