CN115786362B - Heat shock protein family gene HSP110-3 for controlling rice quality and application thereof - Google Patents
Heat shock protein family gene HSP110-3 for controlling rice quality and application thereof Download PDFInfo
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Abstract
The invention discloses a heat shock protein family gene HSP110-3 for controlling rice quality and application thereof. The nucleotide sequence of the gene is shown as SEQ ID NO. 1; the amino acid sequence of the protein coded by the gene is shown as SEQ ID NO. 2. Compared with wild rice ZH11, the transgenic lines knocked out by using CRISPR/Cas9 in the heat shock protein family gene HSP110-3 in the rice ZH11 background show that the grain chalkiness rate is obviously improved, and the heat shock protein family gene HSP110-3 participates in and regulates chalkiness formation, which is a gene for controlling rice quality.
Description
Technical Field
The invention belongs to the technical field of genetic engineering, and particularly relates to a heat shock protein family gene HSP110-3 for controlling rice quality and application thereof.
Background
Rice is one of the most important grain crops in the world, and more than 100 countries and regions are planted in the world. Along with the continuous improvement of the living standard of people, the requirements on the quality of rice are higher and higher, and the continuous improvement of the quality of rice is always a breeding target pursued by rice breeders. The quality of rice mainly comprises four main aspects of grinding quality, appearance quality, nutrition quality and cooking taste quality, the chalkiness of rice are important indexes for evaluating the appearance quality of rice, factors influencing the appearance quality of rice are many, the chalkiness are mainly determined by genetic genes, and the variety-to-variety differences are large. Therefore, the gene for controlling the chalkiness of the rice is developed, the formation mechanism of the chalkiness of the rice is analyzed, and the gene is applied to cultivation of high-quality varieties of cereal crops such as rice and the like, and has important theoretical significance and production application potential.
Over the last 20 years, QTL affecting rice quality has been gradually discovered and clonally studied with rapid development of DNA markers and genome sequencing technologies. The main causes affecting chalky formation are genetic and environmental factors. Genetic factors are mainly starch synthesis related genes such as: wx, AGPase, SSI, SSII, SIII, etc., these genetic mutations can directly lead to a hindered synthesis of rice starch, and thus to a direct formation of grain chalkiness; protein synthesis and transport related genes such as: mutations in OsAAP6, osGluA2, osRab5a, osVPEI, etc., which cause a blockage in protein synthesis and transport processes, indirectly affecting protein body formation and causing chalkiness; fatty acid synthesis and transport genes such as: mutations in genes such as OsLTPL36, LOX3 and OsFAD2-1 lead to the formation of chalky surfaces due to the inhibition of lipid synthesis and transport. In addition, genes affected by high temperature environmental influences such as OsbZIP58, FLO2, osMSD7, cpHSP70-2 directly or indirectly affect the expression of genes related to the synthesis of some nutrients, which in turn leads to chalky formation of the seed. It is almost impossible to greatly reduce the chalkiness of rice by using a QTL or gene for controlling the chalkiness, and polymerization of a plurality of advantageous genes is an effective way to reduce chalkiness, and development of quality improvement of rice by using trait association is a simple and effective method for reducing chalkiness of rice. The genes which are found to control chalkiness are not enough, more genes which control chalkiness are required to be cloned or more low chalkiness are required to be developed, and practical molecular markers closely linked with chalkiness or functional markers in genes are required to be used for molecular breeding for improving chalkiness. Therefore, the research on genes involved in and regulating chalkiness formation has important research significance for improving rice quality.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a heat shock protein family gene HSP110-3 for controlling rice quality and application thereof, and the gene participates in and regulates chalky formation, thereby being beneficial to improving rice quality.
In order to achieve the above purpose, the technical scheme adopted by the invention for solving the technical problems is as follows:
a heat shock protein family gene HSP110-3 for controlling rice quality has a nucleotide sequence shown in SEQ ID NO. 1.
A protein coded by heat shock protein family gene HSP110-3 has an amino acid sequence shown in SEQ ID NO. 2.
Further, the heat shock protein family gene HSP110-3 is applied to the regulation of rice quality.
Further, the heat shock protein family gene HSP110-3 is applied to cultivation of cereal crop varieties such as rice and the like.
An expression vector comprising heat shock protein family gene HSP110-3.
An engineering bacterium comprising a heat shock protein family gene HSP110-3.
A biological agent for regulating rice quality comprises heat shock protein family gene HSP110-3.
The beneficial effects of the invention are as follows:
the invention clones a heat shock protein gene HSP110-3 for controlling rice quality, and after the gene HSP110-3 in wild rice is knocked out by CRISPR/Cas9 gene editing technology, the chalk rate of transgenic plant grains is obviously increased, which indicates that the gene participates in and regulates the formation of chalk, has the function of regulating rice quality, can be used for cultivating high-quality varieties of cereal crops such as rice and the like, and provides reference for cloning and researching homologous genes in other species.
Drawings
FIG. 1 shows the HSP110-3 gene expression profile in rice during the whole growth period.
Figure 2 is a CRISPR/Cas9 system vector map.
FIG. 3 is the HSP110-3 gene CRISPR/Cas9 target site information.
FIG. 4 is a map of the reporter GUS vector.
FIG. 5 shows the results of staining different tissue parts of GUS transgenic plants of HSP110-3.
FIG. 6 shows HSP110-3 transgenic line phenotype and test data; wherein a is a plant leaf shape comparison diagram of the transgenic strain and the wild type strain; b is a graph comparing the ear parts of the transgenic strain with the wild strain; c is a grain length and grain width comparison graph of the transgenic strain and the wild strain; d is a plot of the chalky area of brown rice comparing the transgenic strain to the wild-type strain; e is a polished rice appearance comparison graph of the transgenic strain and the wild strain; f is the ear length comparison of the transgenic line with the wild type line; g is the tiller number of the transgenic strain compared with that of the wild type strain; h is the plant height of the gene strain compared with the wild strain; i is the grain length comparison of the transgenic line and the wild line; j is the grain width comparison of the transgenic line and the wild line; k is the chalky rate of the transgenic line compared to the wild type line; l is the chalky area of the grain of the transgenic line compared with the wild line; m is the chalkiness of the transgenic line compared with the polished rice of the wild line; n is the whiteness of polished rice of the transgenic strain compared with that of the wild strain; o is the transparency of polished rice of the transgenic line compared with that of the wild line; "x" indicates that the difference is significant at the 0.01 level.
Detailed Description
The following description of the embodiments of the present invention is provided to facilitate understanding of the present invention by those skilled in the art, but it should be understood that the present invention is not limited to the scope of the embodiments, and all the inventions which make use of the inventive concept are protected by the spirit and scope of the present invention as defined and defined in the appended claims to those skilled in the art.
Examples
1. qPCR primer design
HSP110-3 protein is composed of 578 amino acids, and the corresponding gene contains 1737 nucleotides; a pair of quantitative primers, LHY53F (5'-GCTGGCAATGTCCTCAGGGTAT-3'), LHY53R (5'-CAGGTCAAGCGTACTGCCATACA-3'), were designed in the exon region of the HSP110-3 gene, respectively, using software Primer 5.
2. HSP110-3 gene space-time expression profile analysis of rice whole growth period
RNA in rice roots, leaves in seedling stage, flag leaves in grouting stage, stems, internodes, young spikes 3cm, young spikes 12cm and caryopsis 5d, 10d, 15d, 20d and 25d after flowering is extracted, and expression analysis is performed by qPCR after reverse transcription, as shown in figure 1.
The results show that: HSP110-3 gene is mainly expressed in caryopsis and is highly expressed in the middle and later stages of caryopsis development.
3. Construction and genetic transformation of CRISPR/Cas9 knockout vector of HSP110-3 gene
(1) Design and selection of guide RNA target site sequences
Based on the genomic sequence of HSP100-3, 1 guide RNA target sequence of HSP110-3 was designed and synthesized.
(2) Target site CRISPR/Cas9-gRNA vector construction
Denaturing the oligonucleotide chain of the guide RNA target sequence synthesized in the step (1) at 95 ℃, then mixing the oligonucleotide chain with a pYL-U6a-gRNA intermediate vector, adding Bsa1 endonuclease, T4 ligase and buffer, carrying out enzyme digestion connection in 5min at 37 ℃ and 5min at 20 ℃ on a PCR instrument, obtaining 2 gRNA expression cassettes respectively containing the target site through two rounds of PCR, and sequentially loading the gRNA expression cassettes onto a CRISPR/Cas9 vector through a Golden gate cloning method to obtain a single target site CRISPR/Cas9-gRNA vector and a CRISPR/Cas9 system vector map as shown in figure 2; HSP110-3 gene CRISPR/Cas9 target site information as shown in FIG. 3.
(3) Genetic transformation
Seed 11 of Chinese flowering plant is sterilized by sodium hypochlorite solution and placed on an induction culture medium, induced calli are picked up for subculture after about 2 weeks, and calli with vigorous growth are picked up for agrobacteria infection after 1 week.
Transfer the CRISPR/Cas9-gRNA vector with the knocked-out target site into the agrobacterium strain EH105, pick up the monoclonal shaking bacteria, dip the callus, co-culture for 3 days at 25 ℃ under dark condition, and then transfer the cultured callus to a screening culture medium containing G418 antibiotics for screening for about 10 days. Transferring the selected calli to a differentiation medium for culture, and removing the calli from a test tube for transplanting after the calli grow into normal seedlings. And finally, extracting DNA from plant leaves, and carrying out positive plant identification by PCR.
4. Positive plant detection
Extracting DNA from plant leaves obtained in the step (3), screening by using hygromycin detection primers to obtain positive plants, amplifying by using cross-target site amplification primers AK773 (5'-AGCATTCCTCACCGGATGTT-3') and AK774 (5'-AGTCGCAGATGTCGTTTCCT-3') respectively, and sequencing the amplified sequences in the positive single plants by the engine sequencing company.
Sequencing the positive plants to obtain sequences, and comparing and analyzing the sequences to show that HSP110-3 genes obtain transgenic plants with 2 premature protein translation termination and 1 amino acid deletion.
5. GUS vector construction and transformation
A2 kb (HSP 110-3) genomic fragment upstream of the ATG start codon was amplified by PCR using the DNA of wild-type ZH11 as template, and the PCR products were ligated with the linearized DX2181 vector using a single-fragment recombinase for gel recovery. And delivering the hundred-cell gene transgenic japonica rice ZH11 material. GUS vector map as shown in FIG. 4.
6. GUS material staining
And (5) taking each tissue and organ of the positive GUS transgenic plant for GUS staining analysis.
(1) The X-Gluc Solution (50X) and GUS Buffer were thoroughly mixed in a ratio of 1:50 to prepare an X-Gluc staining Solution.
(2) Each tissue was placed in a 5ml centrifuge tube, and an appropriate amount of staining solution was added to completely submerge the tissue in the staining solution.
(3) Incubate at 37℃in the dark for 1-24h until blue color appears on the tissue.
(4) The chlorophyll in the tissues is removed repeatedly by 70% ethanol until the chlorophyll is completely removed.
(5) Samples were stored in 70% ethanol and observed and photographed using a split microscope.
As shown in fig. 5.
7. Phenotypic analysis
In the planting of T0 and T1 generation transgenic plants, the agronomic traits of different strains are observed, and compared with the wild ZH11, the transgenic plants have no obvious difference in other agronomic traits except chalkiness. After obtaining a T2 generation transgenic plant with stable inheritance, three times of field planting are repeated, 2 rows are planted in each repetition, and the target site mutation is confirmed by sequencing and detection. Subsequently, when investigating agronomic traits, each row and hybrid plant was repeatedly removed, 5-10 stable plant lines were selected, the chalky grain rate of individual plants was examined, and statistical analysis was performed, as shown in fig. 6.
The results show that the transgenic plants have significantly increased grain chalkiness compared to wild-type ZH 11. Therefore, the HSP110-3 gene is a rice grain chalkiness regulating gene, and the HSP110-3 gene can regulate rice quality.
Claims (2)
1. Heat shock protein family genesHSP110-3Application of the heat shock protein family gene in regulating and controlling the chalky grain rate of rice grainsHSP110-3The nucleotide sequence of (2) is shown as SEQ ID NO. 1.
2. Heat shock protein family genesHSP110-3Application in breeding varieties with improved chalky rice grain characteristics, and gene of heat shock protein familyHSP110-3The nucleotide sequence of (2) is shown as SEQ ID NO. 1.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1925672A1 (en) * | 2001-06-22 | 2008-05-28 | Syngeta Participations AG | Abiotic stress responsive polynucleotides and polypeptides |
| CN102782141A (en) * | 2009-12-03 | 2012-11-14 | 巴斯夫植物科学有限公司 | Expression cassettes for embryo-specific expression in plants |
| CN102812121A (en) * | 2010-01-25 | 2012-12-05 | 文特里亚生物科学公司 | Methods & compositions for improving protein production |
| CN105874070A (en) * | 2013-09-13 | 2016-08-17 | 不来梅大学 | Transgenic plants for nitrogen fixation |
| CN112831478A (en) * | 2021-02-19 | 2021-05-25 | 四川农业大学 | Protein OsCAT8 for regulating rice chalkiness and coding gene and application thereof |
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| US7365185B2 (en) * | 2000-07-19 | 2008-04-29 | Monsanto Technology Llc | Genomic plant sequences and uses thereof |
| US20110131679A2 (en) * | 2000-04-19 | 2011-06-02 | Thomas La Rosa | Rice Nucleic Acid Molecules and Other Molecules Associated with Plants and Uses Thereof for Plant Improvement |
| JP2005185101A (en) * | 2002-05-30 | 2005-07-14 | National Institute Of Agrobiological Sciences | VEGETABLE FULL-LENGTH cDNA AND UTILIZATION THEREOF |
| US7732667B2 (en) * | 2003-08-27 | 2010-06-08 | Syngenta Participations Ag | Transgenic plants and progeny and seed thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1925672A1 (en) * | 2001-06-22 | 2008-05-28 | Syngeta Participations AG | Abiotic stress responsive polynucleotides and polypeptides |
| CN102782141A (en) * | 2009-12-03 | 2012-11-14 | 巴斯夫植物科学有限公司 | Expression cassettes for embryo-specific expression in plants |
| CN102812121A (en) * | 2010-01-25 | 2012-12-05 | 文特里亚生物科学公司 | Methods & compositions for improving protein production |
| CN105874070A (en) * | 2013-09-13 | 2016-08-17 | 不来梅大学 | Transgenic plants for nitrogen fixation |
| CN112831478A (en) * | 2021-02-19 | 2021-05-25 | 四川农业大学 | Protein OsCAT8 for regulating rice chalkiness and coding gene and application thereof |
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