CN115786080A - Laboratory bacterial automatic culture device - Google Patents

Abstract

The invention discloses an automatic cultivation device for laboratory strains, which comprises a tank body and a driving device. The tank body is sealed and rotatably provided with a hollow ventilation shaft. The driving device is arranged outside the tank and is located outside the tank together with the ventilation shaft. One end of the ventilation shaft is driven and connected to drive the rotation of the ventilation shaft. The end of the ventilation shaft outside the tank is also used to inject sterile air into the inner cavity of the ventilation shaft. The end of the ventilation shaft inside the tank is provided with a stirrer , the inside of the stirrer has a ventilating passage communicated with the inner chamber of the ventilating shaft, the stirrer is provided with an air outlet part communicating with the ventilating passage, the air outlet faces the bottom of the tank body and has a gap with it, so that the present invention will ventilate and Stirring as a whole, by ventilating while stirring and dispersing the sterile air injected into the tank to every position of the activation solution in a bottom-up manner, so that the bacteria in the tank and the activation solution in the tank are fully mixed The effect makes the activation and amplification effect of the bacteria better.

Description

A laboratory strain automatic cultivation device

technical field

The invention relates to the field of strain cultivation equipment, in particular to a laboratory strain automatic cultivation device.

Background technique

Laboratory strains include active dry yeast, and active dry yeast often needs to be activated and cultured in a Karst jar. In the existing method of activating yeast strains through the Karst tank, when the yeast activation liquid in the Karl Fischer tank needs to be stirred, the motor is generally used to drive the stirring blades to stir in the Karl Fischer tank to achieve the best stirring effect. Purpose: When the yeast strain in the tank needs to be activated and enlarged, it is necessary to feed clean air into the activation solution in the tank through the oxygen supply hole on the tank, as disclosed in a Chinese patent (notification number: CN206328395U). Yeast strain cultivation cartridges. It can be seen that the structure of the existing culture device for activating yeast strains, its stirring and aeration are separated independently, so the dissolved oxygen effect of the yeast strains will be caused to be poor and the mixing of the yeast strains and the activation solution will not be good. The effect of fully activating and amplifying the strain is poor.

Contents of the invention

Aiming at the deficiencies in the prior art, the object of the present invention is to provide a laboratory strain automatic culture device, which takes aeration and stirring as a whole, and allows the non-fertilized bacteria that will be injected into the tank to be aerated while stirring and from bottom to top. Bacterial air is dispersed to every position of the activation solution, so that the bacteria in the tank and the culture solution in the tank can be fully mixed, so that the activation and amplification effect of the bacteria is better.

The purpose of the present invention adopts following technical scheme to realize:

An automatic cultivation device for laboratory strains, comprising a tank body and a driving device, the tank body is sealed and rotatably provided with a hollow ventilation shaft, and the driving device is arranged outside the tank body and positioned with the ventilation shaft One end of the outside of the tank is driven and connected to drive the rotation of the ventilation shaft. The end of the ventilation shaft outside the tank is also used to inject sterile air into the inner cavity of the ventilation shaft. The ventilation shaft is located at One end of the tank body is provided with an agitator, and the inside of the agitator has an air passage communicated with the inner cavity of the aeration shaft, and the agitator is provided with an air outlet part communicated with the air passage. The air outlet part faces the bottom of the tank and has a gap therewith.

Further, the bottom of the tank body is concave to form a first flat portion and a concave cavity connected to each other, the bottom of the agitator is provided with a raised portion extending into the concave cavity, and the gas outlet is provided On the raised part, the distance between the raised part and the bottom of the concave cavity is d1, then the relationship d1 should satisfy is 0.3≤d1≤0.5cm, the side wall on the raised part and the concave cavity There is a gap between the side walls in the cavity.

Further, a second flat part is formed on the bottom of the agitator, and the second flat part is located outside the raised part, and a flow guide is formed between the first flat part and the second flat part. aisle.

Further, the distance between the agitator and the side wall in the tank is d2, and the relationship d2 should satisfy is 0.5≤d2≤1cm.

Further, the air outlet part includes a plurality of air outlet holes, and the interval between two adjacent air outlet holes is uniform.

Further, the ventilation channel includes a first U-shaped channel and a second U-shaped channel arranged relatively symmetrically, and one end of the first U-shaped channel and the second U-shaped channel communicates with the inner cavity of the ventilation shaft respectively, The other ends of the first U-shaped channel and the second U-shaped channel communicate with opposite ends of the air outlet respectively.

Further, the tank body includes a tank body and a tank cover, the tank cover is detachably connected to the tank body by means of a rotating buckle, and a polyurethane insulation layer is provided on the outer peripheral side wall of the tank body.

Further, one end of the ventilation shaft located outside the tank is also used for sealing and rotatable communication with the fermenter, and the top of the tank is sealed and connected with a pressure tube for injecting sterile compressed air into the tank.

Further, the top of the tank body is provided with a feed liquid injection channel, and the feed liquid injection channel is sealed and rotatably provided with a cap with holes, and the holes of the cap are used to communicate the inside of the tank with the outside world, The hole of the cap is sealed by a silica gel plug, and a first control valve and a second control valve are arranged in the feed liquid injection channel, and switching between the first control valve and the second control valve is used to balance the pressure inside the tank.

Further, the ventilation shaft is provided with a sanitary valve, the opening and closing of the sanitary valve is used to control the on-off of the steam input into the ventilation shaft, and the tank body is also sealed and connected to the steam in the tank. exhaust pipe.

Compared with the prior art, the beneficial effects of the present invention are:

When the present invention is in use, when the ventilation shaft is driven by the driving device to drive the stirrer to stir the strain activation liquid in the tank, the sterile air enters the inner cavity of the ventilation shaft, the ventilation channel and the air outlet in sequence, because the air outlet faces The bottom of the tank has a gap with it, so the sterile air is dispersed from bottom to top in the tank to every position of the activation solution while the agitator is stirring the strain activation solution. That is to say, the present invention regards aeration and stirring as a whole, and allows the sterile air injected into the tank to disperse to every position of the activation solution by aerating while stirring and from bottom to top, so that the bacteria in the tank are in harmony with the The culture solution in the tank achieves the effect of sufficient mixing, so that the activation and amplification effect of the bacteria is better.

Description of drawings

Fig. 1 is the structural representation of laboratory strain automatic cultivation device of the present invention;

Fig. 2 is a top view of an automatic cultivation device for laboratory strains related to an embodiment of the present invention;

Fig. 3 is a schematic diagram of the structure of the embodiment of the present invention involving the connection between the ventilation shaft and the single agitator;

Fig. 4 is a schematic structural diagram of an embodiment of the present invention related to the connection between the ventilation shaft and the double agitator;

Fig. 5 is a schematic diagram of the connection structure between the feed liquid injection channel and the cap according to the embodiment of the present invention.

In the figure: 10, tank body; 101, first flat part; 102, concave cavity; 11, tank body; 110, polyurethane insulation layer; 12, tank cover; 121, feed liquid injection channel; 1211, cap; 1212, Silicone plug; 1213, first control valve; 1214, second control valve; 122, interface; 123, hook; 20, driving device; 30, ventilation shaft; 31, sanitary valve; 40, agitator; 401, first U-shaped channel; 402, second U-shaped channel; 403, second flat part; 41, air outlet; 411, air outlet; 42, raised part; 431, diversion channel; 50, thermometer; 60, sampling valve.

Detailed ways

In the following, the present invention will be firstly described in conjunction with the accompanying drawings and specific implementation methods. It should be noted that, under the premise of not conflicting, the various embodiments or technical features described below can be combined arbitrarily to form new embodiments. .

In describing the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "horizontal", "vertical", "top", The orientation or positional relationship indicated by "outside" is based on the orientation or positional relationship shown in the drawings, which is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the referred device or element must have a specific orientation, are constructed and operate in a particular orientation and therefore are not to be construed as limitations of the invention. In addition, the terms "first" and "second" are used for descriptive purposes only, and should not be understood as indicating or implying relative importance.

In the description of the present invention, it should be noted that, unless otherwise clearly stipulated and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be fixed connection or detachable connection, Or integrally connected; it may be mechanically connected or electrically connected; it may be directly connected or indirectly connected through an intermediary, or the internal communication of two components. Those of ordinary skill in the art can understand the specific meanings of the above terms in the present invention according to specific situations.

Implementation method:

Please refer to Fig. 1-Fig. 5, the present invention has shown a kind of laboratory strain automatic cultivating device, comprises tank body 10 and driving device 20, is provided with hollow ventilation shaft 30 sealingly and rotatably on tank body 10, and driving device 20 is located outside the tank body 10 and connected to the one end of the ventilation shaft 30 outside the tank body 10 for driving the rotation of the ventilation shaft 30. The inner chamber of 30 is injected with sterile air, and the end of the ventilation shaft 30 located in the tank body 10 is provided with an agitator 40, and the inside of the agitator 40 has a ventilation passage communicated with the inner cavity of the aeration shaft 30, and the agitator 40 is provided with a The air outlet portion 41 communicated with the air passage, the air outlet portion 41 faces the bottom of the tank body 10 and has a gap therewith. When the present invention is in use, the drive device 20 drives the ventilation shaft 30 to drive the stirrer 40 to stir the strain activation solution in the tank body 10, and at the same time, the sterile air enters the inner cavity of the ventilation shaft 30, the ventilation channel and the air outlet 41 in turn. Inside the body 10, since the air outlet part 41 faces the bottom of the tank body 10 and there is a gap with it, the sterile air is dispersed from bottom to top in the tank body 10 to activate the strain while the agitator 40 is stirring the strain activation solution. every position of the liquid. That is to say, the present invention regards aeration and stirring as a whole, and allows the sterile air injected into the tank body 10 to disperse to every position of the activation solution by aeration while stirring and from bottom to top, so that the inside of the tank body 10 The bacterial strains and the culture solution in the tank body 10 achieve a sufficient effect of mixing, so that the activation and amplification effect of the bacterial strains is better.

In this embodiment, the air outlet part 41 includes a plurality of air outlet holes 411, and the interval between two adjacent air outlet holes 411 is uniform, and the uniformly distributed air outlet holes 411 can evenly distribute sterile air in the feed liquid to achieve uniform dissolution. purpose of oxygen.

It is worth noting that the driving device 20 of this embodiment selects a variable frequency motor, and drives the aeration shaft 30 through the variable frequency motor to drive the agitator 40 to rotate at a variable speed, so as to solve the problem of uneven mixing during the activation or expansion of the bacteria in the traditional Karst tank, and at the same time Solve the problem of loss due to powdery strains being dissolved until the feed liquid is insufficient and sinks to the bottom.

In this embodiment, the bottom of the tank body 10 is recessed to form a first flat portion 101 and a concave cavity 102 that are connected to each other. First flat portions 101 are formed on both sides of the bottom wall inside the body 10 . The bottom of the agitator 40 is provided with a raised portion 42 extending into the concave cavity 102, the air outlet 41 is arranged at the bottom of the raised portion 42, and the distance between the raised portion 42 and the bottom of the concave cavity 102 is d1, then d1 The relationship to be satisfied is 0.3≤d1≤0.5cm. Certainly, there is a gap between the side wall on the raised portion 42 and the side wall in the concave cavity 102 , so that the liquid in the tank body 10 can enter between the raised portion 42 and the bottom of the concave cavity 102 through the gap.

It should be noted that d1 of the present embodiment is 0.3cm or 0.4cm or 0.5cm. After the bacteria are activated in the tank body 10 (sterile air stops injecting into the tank body 10), the pressure difference in the tank body 10 When the material in the tank body 10 is hydraulically fed to the fermenter (not shown), the material liquid in the tank body 10 is collected in the concave cavity 102, and then the material in the tank body 10 is hydraulically transferred to the fermenter (not shown in the figure) through the gas outlet 41, correspondingly Compared with the liquid draining structure of the traditional Karl Fischer tank, the present invention controls the distance d1 between the raised portion 42 and the bottom of the concave cavity 102 within the above-mentioned range, which can reduce the residue of feed liquid at the bottom of the tank body 10 . Specifically, one end of the ventilation shaft 30 located outside the tank body 10 is also used to seal and rotate in communication with the fermenter, and the top of the tank body 10 is sealed and connected with a pressure tube for injecting sterile compressed air into the tank body 10 (not shown in the figure). shown), by injecting sterile compressed air into the tank body 10, a pressure difference is formed between the tank body 10 of this embodiment and the inside of the fermenter; in addition, by injecting sterile compressed air into the tank body 10, the tank body 10 can also be The material in the body 10 is hydraulically injected into the concave cavity 102, and the pressure difference between the inside of the tank body 10 of this embodiment and the inside of the fermenter can be used to make the material liquid in the tank body 10 of this embodiment be pressed to In the fermenter, prevent the bacteria in the external environment from contaminating the strain activation liquid (the mixture of the strain and the culture liquid) in the tank body 10 .

However, the drain structure in the traditional Karst tank includes a drain tube (not shown), the drain tube is in contact with the bottom flat surface of the Karl Fischer tank, and the bottom end of the drain tube is provided with a 30-45° cut , which uses negative pressure to press the feed liquid in the Karl Fischer tank into the fermentation tank through the cutout of the discharge pipe. Generally speaking, the liquid discharge structure in the traditional Karl Fischer tank pressurizes the material in the Karl Fischer tank to the fermenter and the height of the liquid remaining in the Karl Fischer tank is 0.8-1 cm. Of course, the tank body 10 of this embodiment It is the same as the length and width of the Karl Fischer tank, and the drainage structure of the present invention hydraulically presses the material in the tank body 10 into the fermenter, and the height remaining in the concave cavity 102 is 0.3 cm or 0.4 cm or 0.5 cm, and The length of the concave cavity is less than the length in the Karl Fischer tank, then on the basis that the width in the Karl Fischer tank is the same as the width in the tank body 10 of the present embodiment, since the height of the feed liquid remaining in the tank body 10 is 0.3cm Or 0.4cm or 0.5cm, and the length of the concave cavity 102 is less than the length of the bottom of the Karst tank, so the material in the tank body 10 is hydraulically fed to the fermenter through the drainage structure of the present invention, which can greatly reduce the residue of the material liquid.

In this embodiment, a second flat portion 403 is formed on the bottom of the agitator 40, the second flat portion 403 is located outside the raised portion 42, and a flow guide channel is formed between the first flat portion 101 and the second flat portion 403 431 , through the diversion channel 431 , the strain activation solution in the tank body 10 can be diverted into the concave cavity 102 . It can be seen that the present invention prevents the material liquid at the edge of the side wall in the tank body 10 from entering the bottom of the air outlet part 41 by combining the diversion channel 431 with the concave cavity 102 at the inner bottom of the tank body 10 . Specifically, it can be understood that if the entire bottom wall of the tank body 10 is a flat structure, the feed liquid at the edge of the side wall in the tank body 10 may not be able to reach the position between the raised portion 42 and the inner bottom wall of the tank body 10 .

In this embodiment, the distance between the agitator 40 and the side wall of the tank body 10 is d2, and the relationship d2 should satisfy is 0.5≦d2≦1cm. In this embodiment, d2 is 0.8 cm, so that the agitator 40 can control the vortex in the process of agitating the material liquid, which will affect the mixing effect of the strain and the culture solution. Of course, in other embodiments, d2 may also be 0.5 cm or 1 cm, which is not limited here.

In this embodiment, the ventilation channel of the agitator 40 includes a first U-shaped channel 401 and a second U-shaped channel 402 which are relatively symmetrically arranged, and one end of the first U-shaped channel 401 and the second U-shaped channel 402 are connected to the ventilation shaft respectively. The inner cavity of 30 communicates with each other, and the other ends of the first U-shaped channel 401 and the second U-shaped channel 402 communicate with opposite ends of the air outlet 41 respectively. It can be seen that, during the pressing process, the bacteria activation solution in the tank body 10 branches into the first U-shaped channel 401 and the second U-shaped channel 402 from the concave cavity 102 through the air outlet 41, and then collects in the ventilated channel. The inner chamber of the shaft 30 is pressed into the fermenter, and through this ventilation channel structure, the material liquid entering the ventilation channel can be stirred by the rotation of the agitator 40 when pressing the material, thus playing a role of pressing while stirring. The effect of increasing the aerobic solubility of the feed liquid in the tank body 10.

It should be noted that the number of agitators 40 is not limited to the two as defined in the accompanying drawings, and the specific situation is reasonably selected by the inventor according to the amount of feed liquid prepared in the tank body 10, so as to make the culture of bacteria more uniform and thorough . Certainly, the structure of each agitator 40 is the same, that is, the structure of the air passage in each agitator 40 is the same, so it can be seen that by arranging a plurality of agitators 40, the stirring effect can not only be increased, but also the tank can be increased when pressing the material. The amount of dissolved oxygen in the feed liquid in the body 10.

In this embodiment, the can body 10 includes a can body 11 and a can lid 12, and the can lid 12 is detachably connected to the can body 11 through a rotating buckle. Specifically, the can body 11 and the lid plate are connected by four hooks 123 Fixing or dismounting by rotation facilitates disassembly and cleaning of the tank body 10 . The outer peripheral side wall of the tank body 11 is provided with a polyurethane insulation layer 110, because the material insulation effect of polyurethane is better than that of other insulation materials, so the feed liquid in the tank body 10 can be treated by the polyurethane insulation layer 110 in this embodiment. Play a very good role in heat preservation. Of course, a thermometer 50 is also sealed on the tank body 11 , and the temperature inside the tank body 10 can be known in real time through the thermometer 50 . In addition, a sampling valve 60 is sealed on the tank body 11 , and the feed liquid in the tank body 10 is sampled through the sampling valve 60 .

In this embodiment, the top of the tank body 10 is provided with a feed liquid injection channel 121, that is, the feed liquid injection channel 121 is arranged on the top surface of the cover plate, and the feed liquid injection channel 121 is sealed and rotatably provided with a cap with a hole. 1211, the hole of the cap 1211 is used to communicate the inside of the tank body 10 with the outside world, and the hole of the cap 1211 is sealed by a silicone plug 1212, that is, the position with a hole in the cap 1211 can be inserted into a food-grade silicone plug 1212 for use without Bacteria syringe injects bacteria solution. In addition, a first control valve 1213 and a second control valve 1214 are provided in the feed liquid injection channel 121 , and switching between the first control valve 1213 and the second control valve 1214 is used to balance the pressure in the tank body 10 . Wherein, the first control valve 1213 and the second control valve 1214 are the first manual butterfly valve and the second manual butterfly valve respectively, so it can be understood that when the bacterial liquid is injected into the tank body 10, the food grade silicone plug 1212 is removed, Open the first manual butterfly valve and close the second manual butterfly valve so that the inside of the tank body 10 remains sealed with the outside world. When the bacteria liquid enters the feed liquid injection channel 121 (between the first manual butterfly valve and the second manual butterfly valve), the lid Put on the silica gel plug 1212, and then open the second manual butterfly valve, so that the bacteria solution enters the tank body 10 and mixes with the culture solution in the tank body 10. Of course, when injecting the powdery bacteria into the tank body 10, the rotatable cap 1211 can be opened, the first manual butterfly valve is opened, and the second manual butterfly valve is closed, so that the powdery bacteria enter the feed liquid injection channel 121, and then Close the first manual butterfly valve, open the second manual butterfly valve so that the powdery bacteria can enter the tank body 10, and when the ventilation shaft 30 injects sterile air into the tank body 10, it can pass through the first manual butterfly valve/second manual butterfly valve. Switching between them balances the pressure in the inner chamber of the tank body 10 and eliminates the situation that the air pressure in the tank body 10 blows out the bacteria. Of course, in other embodiments, the first control valve 1213 and the second control valve 1214 may also be electric ball valves, and the switching between them is controlled electrically, which is not limited here.

In this embodiment, the ventilation shaft 30 is provided with a sanitary valve 31, and the opening and closing of the sanitary valve 31 is used to control the on-off of the steam input into the ventilation shaft 30. exhaust pipe. It can be seen that, when the sanitary valve at the upper end of the rotatable ventilation shaft 30 is opened, steam is passed in, the valve on the exhaust pipe is opened, and steam is sterilized in the tank body 10 by continuously passing in steam and discharging. In addition, the exhaust pipe and the raw material pipe share an interface 122 at the sealing connection of the tank body 10, that is, the pressure pipe is sealed and connected to the interface 122, and the exhaust pipe is in sealing communication with the pressure pipe. There are valves on both of them. When pressing the material, the valve on the pressing pipe is opened and the valve on the exhaust pipe is closed; valve opens.

It is worth noting that there are two ways to prepare the materials in the tank body 10: first, the liquid stored in the tank is prepared and then sealed, and heated at the bottom to meet the sterilization temperature, time and temperature requirements for activation; second, Pour feed liquid into the steam sterilized tank body 10 in a sterile environment and then seal the tank.

It should be noted that, the structures of the rotary seals involved in this embodiment all adopt existing rotary seal structures, and no detailed description is given here.

The above-mentioned embodiment is only a preferred embodiment of the present invention, and cannot be used to limit the protection scope of the present invention. Any insubstantial changes and substitutions made by those skilled in the art on the basis of the present invention belong to the scope of the present invention. Scope of protection claimed.

Claims (10)

1. The utility model provides an automatic culture apparatus of laboratory bacterial, includes jar body (10) and drive arrangement (20), characterized in that, jar body (10) go up sealed rotationally be provided with hollow ventilation shaft (30), drive arrangement (20) are located jar body (10) outside and with ventilation shaft (30) are located the one end drive connection outside jar body (10) for the drive ventilation shaft's (30) rotation, ventilation shaft (30) are located the one end outside jar body (10) still is used for to the inner chamber injection sterile air of this ventilation shaft (30), ventilation shaft (30) are located the one end in jar body (10) is provided with agitator (40), the inside of agitator (40) have with the ventilation channel of the inner chamber intercommunication of ventilation shaft (30), be provided with on agitator (40) with ventilation channel intercommunication's portion (41), the portion of giving vent to anger (41) towards the bottom in jar body (10) and have the clearance with it.

2. The automatic laboratory strain culture device according to claim 1, wherein the bottom of the tank (10) is recessed to form a first flat portion (101) and a concave cavity (102) which are connected with each other, a protruding portion (42) extending into the concave cavity (102) is arranged at the bottom of the stirrer (40), the air outlet portion (41) is arranged on the protruding portion (42), the distance between the protruding portion (42) and the bottom of the concave cavity (102) is d1, the d1 satisfies the relationship of 0.3-0.5 cm, and a gap is formed between the side wall of the protruding portion (42) and the side wall of the concave cavity (102).

3. The automatic laboratory strain culture apparatus according to claim 2, wherein a second flat portion (403) is formed at the bottom of the stirrer (40), the second flat portion (403) is located outside the protrusion (42), and a flow guide channel (431) is formed between the first flat portion (101) and the second flat portion (403).

4. The automatic laboratory strain culture apparatus according to claim 3, wherein the distance between the stirrer (40) and the side wall inside the tank (10) is d2, and d2 is 0.5. Ltoreq. D2. Ltoreq.1 cm.

5. The automatic culture apparatus for laboratory strains according to claim 2, wherein the air outlet portion (41) comprises a plurality of air outlet holes (411), and each two adjacent air outlet holes (411) are spaced uniformly.

6. The automatic culture device for laboratory strains according to any one of claims 1 to 5, wherein said ventilation channel comprises a first U-shaped channel (401) and a second U-shaped channel (402) which are symmetrically arranged, one end of said first U-shaped channel (401) and one end of said second U-shaped channel (402) are respectively communicated with the inner cavity of said ventilation shaft (30), and the other end of said first U-shaped channel (401) and the other end of said second U-shaped channel (402) are respectively communicated with the opposite ends of said air outlet portion (41).

7. The automatic laboratory strain culture device according to claim 6, wherein said tank body (10) comprises a tank body (11) and a tank cover (12), said tank cover (12) is detachably connected to said tank body (11) by means of a rotary buckle, and a polyurethane insulating layer (110) is disposed on the peripheral side wall of said tank body (11).

8. The automatic laboratory strain culture device according to claim 6, wherein the end of the ventilation shaft (30) located outside the tank (10) is further used for being in sealed and rotatable communication with the fermentation tank, and the top of the tank (10) is hermetically connected with a material pressing pipe used for injecting sterile compressed air into the tank (10).

9. The automatic culture device for laboratory strains according to claim 1, wherein the top end of the tank (10) is provided with a liquid injection channel (121), the liquid injection channel (121) is rotatably sealed with a cap (1211) having a hole, the hole of the cap (1211) is used for communicating the inside of the tank (10) with the outside, the hole of the cap (1211) is sealed by a silicone plug (1212), the liquid injection channel (121) is internally provided with a first control valve (1213) and a second control valve (1214), and the switching between the first control valve (1213) and the second control valve (1214) is used for balancing the pressure inside the tank (10).

10. The automatic laboratory strain culture device according to claim 1, wherein a sanitary valve (31) is disposed on said ventilation shaft (30), opening and closing of said sanitary valve (31) is used for controlling on and off of steam input into said ventilation shaft (30), and said tank (10) is further hermetically connected with an exhaust pipe for exhausting steam in said tank (10).

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